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P32571

- UBP4_YEAST

UniProt

P32571 - UBP4_YEAST

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Protein

Ubiquitin carboxyl-terminal hydrolase 4

Gene
DOA4, DOS1, MUT4, NPI2, SSV7, UBP4, YDR069C, D4270, YD8554.02C, YD9609.23C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Ubiquitin thioesterase that acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole. Removes also ubiquitin from soluble proteins targeted to proteasomes. Is essential to maintain a normal level of free ubiquitin. Involved in the ammonium-induced down-regulation of the GAP1 permease and the UME3 destruction in response to oxidative stress. Has a role in the RAD9 checkpoint response to TOP1 poisons. Required for promoting coordination of DNA replication and avoids DNA overreplication.19 Publications

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulationi

RFU1 is an inhibitor of deubiquitination activity.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei571 – 5711Nucleophile
Active sitei880 – 8801Proton acceptor By similarity

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. ubiquitin-specific protease activity Source: SGD

GO - Biological processi

  1. endocytosis Source: SGD
  2. free ubiquitin chain depolymerization Source: SGD
  3. intralumenal vesicle formation Source: SGD
  4. regulation of DNA replication Source: SGD
  5. ubiquitin-dependent protein catabolic process Source: SGD
  6. ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD
  7. ubiquitin homeostasis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-29676-MONOMER.

Protein family/group databases

MEROPSiC19.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 4 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 4
Ubiquitin thioesterase 4
Ubiquitin-specific-processing protease 4
Vacuole biogenesis protein SSV7
Gene namesi
Name:DOA4
Synonyms:DOS1, MUT4, NPI2, SSV7, UBP4
Ordered Locus Names:YDR069C
ORF Names:D4270, YD8554.02C, YD9609.23C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDR069c.
SGDiS000002476. DOA4.

Subcellular locationi

Cytoplasm. Late endosome membrane; Peripheral membrane protein
Note: Recruited to the late endosome by BRO1.7 Publications

GO - Cellular componenti

  1. endosome Source: SGD
  2. late endosome membrane Source: UniProtKB-SubCell
  3. proteasome complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi571 – 5711C → S or A: Impairs deubiquitination activity and protein sorting into the MVB pathway. 2 Publications
Mutagenesisi826 – 8261Y → A: Impairs deubiquitination activity and binding to BRO1; when associated with A-827 and A-829. 1 Publication
Mutagenesisi827 – 8271P → A: Impairs deubiquitination activity and binding to BRO1; when associated with A-826 and A-829. 1 Publication
Mutagenesisi829 – 8291L → A: Impairs deubiquitination activity and binding to BRO1; when associated with A-826 and A-827. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 926926Ubiquitin carboxyl-terminal hydrolase 4PRO_0000080589Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei443 – 4431Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32571.
PaxDbiP32571.

Expressioni

Gene expression databases

GenevestigatoriP32571.

Interactioni

Subunit structurei

Interacts with BRO1, RFU1 and VPS32. Associates with the 26S proteasome.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BRO1P4858215EBI-19840,EBI-3768
RFU1Q080032EBI-19840,EBI-2353109

Protein-protein interaction databases

BioGridi32124. 120 interactions.
DIPiDIP-5298N.
IntActiP32571. 27 interactions.
MINTiMINT-550253.
STRINGi4932.YDR069C.

Structurei

3D structure databases

ProteinModelPortaliP32571.
SMRiP32571. Positions 563-922.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini205 – 328124RhodaneseAdd
BLAST
Domaini562 – 923362USPAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi386 – 3927Poly-Gln

Domaini

Residues 1-208 are essential for the localization to the late endosome and constitute a late endosome localization (LEL) domain.1 Publication

Sequence similaritiesi

Belongs to the peptidase C19 family.
Contains 1 rhodanese domain.
Contains 1 USP domain.

Phylogenomic databases

eggNOGiCOG5533.
GeneTreeiENSGT00750000117363.
HOGENOMiHOG000248489.
KOiK11839.
OMAiEDLNQCG.
OrthoDBiEOG7R2BSX.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32571-1 [UniParc]FASTAAdd to Basket

« Hide

MEQNIISTIR DECIRHRSKY LTIAQLTAIA EAKINEFIIT GKAKDQDLSS    50
LLDKCIDILS IYKKNSKDIK NIISCKNKGA MISSNSVMII QLNYVYYKVI 100
HIIVTTNIPH LSEFAKIKLH KSTSDEGNGN NNNNEFQLMN IYNTLLETLL 150
KDENIAKIKS FIKSSIKQTK LNHEQEECNL MRTGSYITSN QLNSLISSSA 200
NSASSQMEIL LIDIRSRLEF NKSHIDTKNI ICLEPISFKM SYSDHDLEKK 250
SLITSPNSEI KMFQSRNLFK FIILYTDANE YNVKQQSVLL DILVNHSFEK 300
PISDDFTKIF ILESGFPGWL KSNYGRQVSS SFPSNNNIKD DSVYINGNTS 350
GLSLQHLPKM SPSIRHSMDD SMKEMLVAPT PLNHLQQQQQ QQSDNDHVLK 400
RSSSFKKLFS NYTSPNPKNS NSNLYSISSL SISSSPSPLP LHSPDPVKGN 450
SLPINYPETP HLWKNSETDF MTNQREQLNH NSFAHIAPIN TKAITSPSRT 500
ATPKLQRFPQ TISMNLNMNS NGHSSATSTI QPSCLSLSNN DSLDHTDVTP 550
TSSHNYDLDF AVGLENLGNS CYMNCIIQCI LGTHELTQIF LDDSYAKHIN 600
INSKLGSKGI LAKYFARLVH MMYKEQVDGS KKISISPIKF KLACGSVNSL 650
FKTASQQDCQ EFCQFLLDGL HEDLNQCGSN PPLKELSQEA EARREKLSLR 700
IASSIEWERF LTTDFSVIVD LFQGQYASRL KCKVCSHTST TYQPFTVLSI 750
PIPKKNSRNN ITIEDCFREF TKCENLEVDE QWLCPHCEKR QPSTKQLTIT 800
RLPRNLIVHL KRFDNLLNKN NDFVIYPFLL DLTPFWANDF DGVFPPGVND 850
DELPIRGQIP PFKYELYGVA CHFGTLYGGH YTAYVKKGLK KGWLYFDDTK 900
YKPVKNKADA INSNAYVLFY HRVYGV 926
Length:926
Mass (Da):105,192
Last modified:February 1, 1994 - v2
Checksum:iB863FE6062510F3C
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti327 – 3271Q → K in AAA35105. 1 Publication
Sequence conflicti345 – 3451I → F in AAA35105. 1 Publication
Sequence conflicti375 – 3784MLVA → TASW in AAA35105. 1 Publication
Sequence conflicti383 – 3831N → I in AAA35105. 1 Publication
Sequence conflicti407 – 4071K → T in AAA35105. 1 Publication
Sequence conflicti543 – 57028LDHTD…NLGNS → FRSYEMLHQLLLIIMTLISR LVWENLEIP in AAA35105. 1 PublicationAdd
BLAST
Sequence conflicti580 – 5801I → T in AAA35105. 1 Publication
Sequence conflicti836 – 8361W → G in AAA35105. 1 Publication
Sequence conflicti897 – 8971D → N in AAA35105. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U02518 Genomic DNA. Translation: AAC48915.1.
L08070 Genomic DNA. Translation: AAA35105.1.
Z49209 Genomic DNA. Translation: CAA89098.1.
Z46796 Genomic DNA. Translation: CAA86791.1.
Z74365 Genomic DNA. Translation: CAA98887.1.
X84162 Genomic DNA. Translation: CAA58985.1.
BK006938 Genomic DNA. Translation: DAA11915.1.
PIRiS39344.
RefSeqiNP_010354.3. NM_001180377.3.

Genome annotation databases

EnsemblFungiiYDR069C; YDR069C; YDR069C.
GeneIDi851641.
KEGGisce:YDR069C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U02518 Genomic DNA. Translation: AAC48915.1 .
L08070 Genomic DNA. Translation: AAA35105.1 .
Z49209 Genomic DNA. Translation: CAA89098.1 .
Z46796 Genomic DNA. Translation: CAA86791.1 .
Z74365 Genomic DNA. Translation: CAA98887.1 .
X84162 Genomic DNA. Translation: CAA58985.1 .
BK006938 Genomic DNA. Translation: DAA11915.1 .
PIRi S39344.
RefSeqi NP_010354.3. NM_001180377.3.

3D structure databases

ProteinModelPortali P32571.
SMRi P32571. Positions 563-922.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32124. 120 interactions.
DIPi DIP-5298N.
IntActi P32571. 27 interactions.
MINTi MINT-550253.
STRINGi 4932.YDR069C.

Protein family/group databases

MEROPSi C19.005.

Proteomic databases

MaxQBi P32571.
PaxDbi P32571.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDR069C ; YDR069C ; YDR069C .
GeneIDi 851641.
KEGGi sce:YDR069C.

Organism-specific databases

CYGDi YDR069c.
SGDi S000002476. DOA4.

Phylogenomic databases

eggNOGi COG5533.
GeneTreei ENSGT00750000117363.
HOGENOMi HOG000248489.
KOi K11839.
OMAi EDLNQCG.
OrthoDBi EOG7R2BSX.

Enzyme and pathway databases

BioCyci YEAST:G3O-29676-MONOMER.

Miscellaneous databases

NextBioi 969209.

Gene expression databases

Genevestigatori P32571.

Family and domain databases

Gene3Di 3.40.250.10. 1 hit.
InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00581. Rhodanese. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view ]
SMARTi SM00450. RHOD. 1 hit.
[Graphical view ]
SUPFAMi SSF52821. SSF52821. 1 hit.
PROSITEi PS50206. RHODANESE_3. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast DOA4 gene encodes a deubiquitinating enzyme related to a product of the human tre-2 oncogene."
    Papa F.R., Hochstrasser M.
    Nature 366:313-319(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. Latterich M.
    Submitted (JAN-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Nucleotide sequence analysis of a 32,500 bp region of the right arm of Saccharomyces cerevisiae chromosome IV."
    Brandt P., Ramlow S., Otto B., Bloecker H.
    Yeast 12:85-90(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 36-926.
    Strain: ATCC 204508 / S288c.
  6. "Coordinating DNA replication to produce one copy of the genome requires genes that act in ubiquitin metabolism."
    Singer J.D., Manning B.M., Formosa T.
    Mol. Cell. Biol. 16:1356-1366(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Ubiquitin lys63 is involved in ubiquitination of a yeast plasma membrane protein."
    Galan J.-M., Haguenauer-Tsapis R.
    EMBO J. 16:5847-5854(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Catabolite inactivation of the yeast maltose transporter requires ubiquitin-ligase npi1/rsp5 and ubiquitin-hydrolase npi2/doa4."
    Lucero P., Lagunas R.
    FEMS Microbiol. Lett. 147:273-277(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Role for the ubiquitin-proteasome system in the vacuolar degradation of Ste6p, the a-factor transporter in Saccharomyces cerevisiae."
    Loayza D., Michaelis S.
    Mol. Cell. Biol. 18:779-789(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "NH4+-induced down-regulation of the Saccharomyces cerevisiae Gap1p permease involves its ubiquitination with lysine-63-linked chains."
    Springael J.-Y., Galan J.-M., Haguenauer-Tsapis R., Andre B.
    J. Cell Sci. 112:1375-1383(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Interaction of the Doa4 deubiquitinating enzyme with the yeast 26S proteasome."
    Papa F.R., Amerik A.Y., Hochstrasser M.
    Mol. Biol. Cell 10:741-756(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: ASSOCIATION WITH THE 26S PROTEASOME.
  12. "The Doa4 deubiquitinating enzyme is required for ubiquitin homeostasis in yeast."
    Swaminathan S., Amerik A.Y., Hochstrasser M.
    Mol. Biol. Cell 10:2583-2594(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "Oxidative stress-induced destruction of the yeast C-type cyclin Ume3p requires phosphatidylinositol-specific phospholipase C and the 26S proteasome."
    Cooper K.F., Mallory M.J., Strich R.
    Mol. Cell. Biol. 19:3338-3348(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. "The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways."
    Amerik A.Y., Nowak J., Swaminathan S., Hochstrasser M.
    Mol. Biol. Cell 11:3365-3380(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  15. "Glucose-induced monoubiquitination of the Saccharomyces cerevisiae galactose transporter is sufficient to signal its internalization."
    Horak J., Wolf D.H.
    J. Bacteriol. 183:3083-3088(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  16. "Uptake of the ATP-binding cassette (ABC) transporter Ste6 into the yeast vacuole is blocked in the doa4 Mutant."
    Losko S., Kopp F., Kranz A., Koelling R.
    Mol. Biol. Cell 12:1047-1059(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  17. "Deubiquitination step in the endocytic pathway of yeast plasma membrane proteins: crucial role of Doa4p ubiquitin isopeptidase."
    Dupre S., Haguenauer-Tsapis R.
    Mol. Cell. Biol. 21:4482-4494(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  18. "Permease recycling and ubiquitination status reveal a particular role for Bro1 in the multivesicular body pathway."
    Nikko E., Marini A.-M., Andre B.
    J. Biol. Chem. 278:50732-50743(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  19. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  20. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  21. "The deubiquitinating enzyme Doa4p protects cells from DNA topoisomerase I poisons."
    Fiorani P., Reid R.J.D., Schepis A., Jacquiau H.R., Guo H., Thimmaiah P., Benedetti P., Bjornsti M.-A.
    J. Biol. Chem. 279:21271-21281(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  22. "Bro1 coordinates deubiquitination in the multivesicular body pathway by recruiting Doa4 to endosomes."
    Luhtala N., Odorizzi G.
    J. Cell Biol. 166:717-729(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH BRO1.
  23. "Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae."
    Bowers K., Lottridge J., Helliwell S.B., Goldthwaite L.M., Luzio J.P., Stevens T.H.
    Traffic 5:194-210(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH VPS32.
  24. "Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes."
    Nickerson D.P., West M., Odorizzi G.
    J. Cell Biol. 175:715-720(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  25. "A conserved late endosome-targeting signal required for Doa4 deubiquitylating enzyme function."
    Amerik A.Y., Sindhi N., Hochstrasser M.
    J. Cell Biol. 175:825-835(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DOMAIN.
  26. "Amino acids regulate retrieval of the yeast general amino acid permease from the vacuolar targeting pathway."
    Rubio-Texeira M., Kaiser C.A.
    Mol. Biol. Cell 17:3031-3050(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  27. "Dual mechanisms specify Doa4-mediated deubiquitination at multivesicular bodies."
    Richter C., West M., Odorizzi G.
    EMBO J. 26:2454-2464(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH BRO1, MUTAGENESIS OF CYS-571; TYR-826; PRO-827 AND LEU-829.
  28. "Split-ubiquitin two-hybrid assay to analyze protein-protein interactions at the endosome: application to Saccharomyces cerevisiae Bro1 interacting with ESCRT complexes, the Doa4 ubiquitin hydrolase, and the Rsp5 ubiquitin ligase."
    Nikko E., Andre B.
    Eukaryot. Cell 6:1266-1277(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BRO1, SUBCELLULAR LOCATION.
  29. "Evidence for a direct role of the Doa4 deubiquitinating enzyme in protein sorting into the MVB pathway."
    Nikko E., Andre B.
    Traffic 8:566-581(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF CYS-571.
  30. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-443, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  31. "An inhibitor of a deubiquitinating enzyme regulates ubiquitin homeostasis."
    Kimura Y., Yashiroda H., Kudo T., Koitabashi S., Murata S., Kakizuka A., Tanaka K.
    Cell 137:549-559(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION, INTERACTION WITH RFU1.

Entry informationi

Entry nameiUBP4_YEAST
AccessioniPrimary (citable) accession number: P32571
Secondary accession number(s): D6VS55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1994
Last modified: June 11, 2014
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 428 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi