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Protein

Mediator of RNA polymerase II transcription subunit 22

Gene

SRB6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-29178-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 22
Alternative name(s):
Mediator complex subunit 22
Suppressor of RNA polymerase B 6
Gene namesi
Name:SRB6
Synonyms:MED22
Ordered Locus Names:YBR253W
ORF Names:YBR1721
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR253W.
SGDiS000000457. SRB6.

Subcellular locationi

GO - Cellular componenti

  • core mediator complex Source: SGD
  • mediator complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi86 – 861N → K in SRB6-1; suppresses the phenotypic defects of an RNA polymerase II CTD truncation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 121120Mediator of RNA polymerase II transcription subunit 22PRO_0000096379Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP32570.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. SRB6/MED22 interacts directly with SRB4/MED17.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MED11Q992786EBI-18039,EBI-27213
SRB4P325695EBI-18039,EBI-18025

Protein-protein interaction databases

BioGridi32948. 57 interactions.
DIPiDIP-1433N.
IntActiP32570. 53 interactions.
MINTiMINT-392894.

Structurei

Secondary structure

1
121
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 2522Combined sources
Helixi42 – 487Combined sources
Helixi50 – 8637Combined sources
Helixi101 – 11616Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J1Oelectron microscopy16.00K1-121[»]
3R84X-ray2.05B/D/F/H/J/L/N/P/R/T/V/X2-89[»]
3RJ1X-ray4.30D/K/R1-121[»]
4GWPX-ray4.20D1-121[»]
4GWQX-ray4.50D1-121[»]
4H62X-ray3.00V96-121[»]
4V1Oelectron microscopy9.70Z2-121[»]
ProteinModelPortaliP32570.
SMRiP32570. Positions 1-121.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mediator complex subunit 22 family.Curated

Phylogenomic databases

HOGENOMiHOG000000733.
InParanoidiP32570.
KOiK15140.
OMAiMMVNNQT.
OrthoDBiEOG7MH1BT.

Family and domain databases

InterProiIPR009332. Med22.
IPR016530. Med22_Saccharomyce.
[Graphical view]
PfamiPF06179. Med22. 1 hit.
[Graphical view]
PIRSFiPIRSF007936. SRB6. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32570-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNQALYEKL EQTRTILSVK LAELINMTTI ADRNDDDEGS FAQENSELAV
60 70 80 90 100
ATTSVMMVNN QTMQLIKNVQ DLLILTRSIK EKWLLNQIPV TEHSKVTRFD
110 120
EKQIEELLDN CIETFVAEKT T
Length:121
Mass (Da):13,863
Last modified:October 1, 1993 - v1
Checksum:i7703E4FAE101A31F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12027 Genomic DNA. Translation: AAB08013.1.
X70529 Genomic DNA. Translation: CAA49917.1.
Z36122 Genomic DNA. Translation: CAA85216.1.
BK006936 Genomic DNA. Translation: DAA07369.1.
PIRiS32956.
RefSeqiNP_009812.1. NM_001178601.1.

Genome annotation databases

EnsemblFungiiYBR253W; YBR253W; YBR253W.
GeneIDi852555.
KEGGisce:YBR253W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12027 Genomic DNA. Translation: AAB08013.1.
X70529 Genomic DNA. Translation: CAA49917.1.
Z36122 Genomic DNA. Translation: CAA85216.1.
BK006936 Genomic DNA. Translation: DAA07369.1.
PIRiS32956.
RefSeqiNP_009812.1. NM_001178601.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J1Oelectron microscopy16.00K1-121[»]
3R84X-ray2.05B/D/F/H/J/L/N/P/R/T/V/X2-89[»]
3RJ1X-ray4.30D/K/R1-121[»]
4GWPX-ray4.20D1-121[»]
4GWQX-ray4.50D1-121[»]
4H62X-ray3.00V96-121[»]
4V1Oelectron microscopy9.70Z2-121[»]
ProteinModelPortaliP32570.
SMRiP32570. Positions 1-121.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32948. 57 interactions.
DIPiDIP-1433N.
IntActiP32570. 53 interactions.
MINTiMINT-392894.

Proteomic databases

MaxQBiP32570.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR253W; YBR253W; YBR253W.
GeneIDi852555.
KEGGisce:YBR253W.

Organism-specific databases

EuPathDBiFungiDB:YBR253W.
SGDiS000000457. SRB6.

Phylogenomic databases

HOGENOMiHOG000000733.
InParanoidiP32570.
KOiK15140.
OMAiMMVNNQT.
OrthoDBiEOG7MH1BT.

Enzyme and pathway databases

BioCyciYEAST:G3O-29178-MONOMER.

Miscellaneous databases

PROiP32570.

Family and domain databases

InterProiIPR009332. Med22.
IPR016530. Med22_Saccharomyce.
[Graphical view]
PfamiPF06179. Med22. 1 hit.
[Graphical view]
PIRSFiPIRSF007936. SRB6. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast."
    Thompson C.M., Koleske A.J., Chao D.M., Young R.A.
    Cell 73:1361-1375(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF ASN-86.
    Strain: Z28.
  2. "The complete sequence of a 19,482 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae."
    Doignon F., Biteau N., Crouzet M., Aigle M.
    Yeast 9:189-199(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II."
    Kim Y.-J., Bjoerklund S., Li Y., Sayre M.H., Kornberg R.D.
    Cell 77:599-608(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPONENT OF MEDIATOR COMPLEX.
  6. "An activator target in the RNA polymerase II holoenzyme."
    Koh S.S., Ansari A.Z., Ptashne M., Young R.A.
    Mol. Cell 1:895-904(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SRB4.
  7. "Interplay of positive and negative regulators in transcription initiation by RNA polymerase II holoenzyme."
    Lee T.I., Wyrick J.J., Koh S.S., Jennings E.G., Gadbois E.L., Young R.A.
    Mol. Cell. Biol. 18:4455-4462(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SRB4.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Association of the Mediator complex with enhancers of active genes."
    Kuras L., Borggrefe T., Kornberg R.D.
    Proc. Natl. Acad. Sci. U.S.A. 100:13887-13891(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: ASSOCIATION WITH PROMOTER REGIONS.
  11. Cited for: NOMENCLATURE.
  12. Cited for: TOPOLOGY OF THE MEDIATOR COMPLEX.
  13. "Preponderance of free mediator in the yeast Saccharomyces cerevisiae."
    Takagi Y., Chadick J.Z., Davis J.A., Asturias F.J.
    J. Biol. Chem. 280:31200-31207(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX.
  14. "Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts."
    Nair D., Kim Y., Myers L.C.
    J. Biol. Chem. 280:33739-33748(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
  15. "Mediator as a general transcription factor."
    Takagi Y., Kornberg R.D.
    J. Biol. Chem. 281:80-89(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
  16. "Med19(Rox3) regulates intermodule interactions in the Saccharomyces cerevisiae mediator complex."
    Baidoobonso S.M., Guidi B.W., Myers L.C.
    J. Biol. Chem. 282:5551-5559(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
  17. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  18. "Structure of the yeast RNA polymerase II holoenzyme: mediator conformation and polymerase interaction."
    Davis J.A., Takagi Y., Kornberg R.D., Asturias F.J.
    Mol. Cell 10:409-415(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: ELECTRON MICROSCOPY OF MEDIATOR COMPLEX IN COMPLEX WITH RNA POLYMERASE II.
  19. Cited for: INTERACTION WITH SRB4, FUNCTION OF THE MEDIATOR COMPLEX HEAD MODULE, ELECTRON MICROSCOPY OF THE MEDIATOR COMPLEX HEAD MODULE, INTERACTION OF THE MEDIATOR COMPLEX HEAD MODULE WITH RNA POLYMERASE II AND TFIIF.

Entry informationi

Entry nameiMED22_YEAST
AccessioniPrimary (citable) accession number: P32570
Secondary accession number(s): D6VQP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 6, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2181 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.