P32570 (MED22_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mediator of RNA polymerase II transcription subunit 22

Alternative name(s):
Mediator complex subunit 22
Suppressor of RNA polymerase B 6

Gene names

Name:	SRB6
Synonyms:	MED22
Ordered Locus Names:	YBR253W
ORF Names:	YBR1721

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	121 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. Ref.14 Ref.15 Ref.18
Subunit structure	Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. SRB6/MED22 interacts directly with SRB4/MED17. Ref.6 Ref.7 Ref.16 Ref.18
Subcellular location	Nucleus Ref.8.
Miscellaneous	Present with 2181 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the Mediator complex subunit 22 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Activator
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.5. Source: SGD
Cellular_component	core mediator complex Inferred from direct assay PubMed 9891034. Source: SGD mediator complex Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA polymerase II transcription cofactor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MED11	Q99278	3	EBI-18039,EBI-27213

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 121

121

Mediator of RNA polymerase II transcription subunit 22

PRO_0000096379

Experimental info

Mutagenesis

86

1

N → K in SRB6-1; suppresses the phenotypic defects of a RNA polymerase II CTD truncation. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

121

Helix Strand Turn

Details...

Helix

4 – 25

22

Helix

42 – 48

7

Helix

50 – 86

37

Helix

101 – 116

16

Sequences

	Sequence		Length	Mass (Da)
	P32570 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 7703E4FAE101A31F	FASTA	121	13,863
	10 20 30 40 50 60 MSNQALYEKL EQTRTILSVK LAELINMTTI ADRNDDDEGS FAQENSELAV ATTSVMMVNN 70 80 90 100 110 120 QTMQLIKNVQ DLLILTRSIK EKWLLNQIPV TEHSKVTRFD EKQIEELLDN CIETFVAEKT T

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast." Thompson C.M., Koleske A.J., Chao D.M., Young R.A. Cell 73:1361-1375(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF ASN-86. Strain: Z28.
[2]	"The complete sequence of a 19,482 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae." Doignon F., Biteau N., Crouzet M., Aigle M. Yeast 9:189-199(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II." Kim Y.-J., Bjoerklund S., Li Y., Sayre M.H., Kornberg R.D. Cell 77:599-608(1994) [PubMed] [Europe PMC] [Abstract] Cited for: COMPONENT OF MEDIATOR COMPLEX.
[6]	"An activator target in the RNA polymerase II holoenzyme." Koh S.S., Ansari A.Z., Ptashne M., Young R.A. Mol. Cell 1:895-904(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SRB4.
[7]	"Interplay of positive and negative regulators in transcription initiation by RNA polymerase II holoenzyme." Lee T.I., Wyrick J.J., Koh S.S., Jennings E.G., Gadbois E.L., Young R.A. Mol. Cell. Biol. 18:4455-4462(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SRB4.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Association of the Mediator complex with enhancers of active genes." Kuras L., Borggrefe T., Kornberg R.D. Proc. Natl. Acad. Sci. U.S.A. 100:13887-13891(2003) [PubMed] [Europe PMC] [Abstract] Cited for: ASSOCIATION WITH PROMOTER REGIONS.
[11]	"A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II." Bourbon H.-M., Aguilera A., Ansari A.Z., Asturias F.J., Berk A.J., Bjoerklund S., Blackwell T.K., Borggrefe T., Carey M., Carlson M., Conaway J.W., Conaway R.C., Emmons S.W., Fondell J.D., Freedman L.P., Fukasawa T., Gustafsson C.M., Han M. , He X., Herman P.K., Hinnebusch A.G., Holmberg S., Holstege F.C.P., Jaehning J.A., Kim Y.-J., Kuras L., Leutz A., Lis J.T., Meisterernest M., Naeaer A.M., Nasmyth K., Parvin J.D., Ptashne M., Reinberg D., Ronne H., Sadowski I., Sakurai H., Sipiczki M., Sternberg P.W., Stillman D.J., Strich R., Struhl K., Svejstrup J.Q., Tuck S., Winston F., Roeder R.G., Kornberg R.D. Mol. Cell 14:553-557(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE.
[12]	"A high resolution protein interaction map of the yeast Mediator complex." Guglielmi B., van Berkum N.L., Klapholz B., Bijma T., Boube M., Boschiero C., Bourbon H.-M., Holstege F.C.P., Werner M. Nucleic Acids Res. 32:5379-5391(2004) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY OF THE MEDIATOR COMPLEX.
[13]	"Preponderance of free mediator in the yeast Saccharomyces cerevisiae." Takagi Y., Chadick J.Z., Davis J.A., Asturias F.J. J. Biol. Chem. 280:31200-31207(2005) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX.
[14]	"Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts." Nair D., Kim Y., Myers L.C. J. Biol. Chem. 280:33739-33748(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
[15]	"Mediator as a general transcription factor." Takagi Y., Kornberg R.D. J. Biol. Chem. 281:80-89(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
[16]	"Med19(Rox3) regulates intermodule interactions in the Saccharomyces cerevisiae mediator complex." Baidoobonso S.M., Guidi B.W., Myers L.C. J. Biol. Chem. 282:5551-5559(2007) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
[17]	"Structure of the yeast RNA polymerase II holoenzyme: mediator conformation and polymerase interaction." Davis J.A., Takagi Y., Kornberg R.D., Asturias F.J. Mol. Cell 10:409-415(2002) [PubMed] [Europe PMC] [Abstract] Cited for: ELECTRON MICROSCOPY OF MEDIATOR COMPLEX IN COMPLEX WITH RNA POLYMERASE II.
[18]	"Head module control of mediator interactions." Takagi Y., Calero G., Komori H., Brown J.A., Ehrensberger A.H., Hudmon A., Asturias F.J., Kornberg R.D. Mol. Cell 23:355-364(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SRB4, FUNCTION OF THE MEDIATOR COMPLEX HEAD MODULE, ELECTRON MICROSCOPY OF THE MEDIATOR COMPLEX HEAD MODULE, INTERACTION OF THE MEDIATOR COMPLEX HEAD MODULE WITH RNA POLYMERASE II AND TFIIF.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L12027 Genomic DNA. Translation: AAB08013.1.
X70529 Genomic DNA. Translation: CAA49917.1.
Z36122 Genomic DNA. Translation: CAA85216.1.
BK006936 Genomic DNA. Translation: DAA07369.1.

PIR

S32956.

RefSeq

NP_009812.1. NM_001178601.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3J1O	electron microscopy	16.00	K	1-121	[»]
3R84	X-ray	2.05	B/D/F/H/J/L/N/P/R/T/V/X	1-89	[»]
3RJ1	X-ray	4.30	D/K/R	1-121	[»]
4GWP	X-ray	4.20	D	1-121	[»]
4GWQ	X-ray	4.50	D	1-121	[»]
4H62	X-ray	3.00	V	96-121	[»]

ProteinModelPortal

P32570.

SMR

P32570. Positions 1-121.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1433N.

IntAct

P32570. 52 interactions.

MINT

MINT-392894.

STRING

4932.YBR253W.

Proteomic databases

PaxDb

P32570.

PeptideAtlas

P32570.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YBR253W; YBR253W; YBR253W.

GeneID

852555.

KEGG

sce:YBR253W.

Organism-specific databases

CYGD

YBR253w.

SGD

S000000457. SRB6.

Phylogenomic databases

eggNOG

NOG85214.

HOGENOM

HOG000000733.

KO

K15140.

OMA

MMVNNQT.

OrthoDB

EOG4KH64T.

Gene expression databases

Genevestigator

P32570.

GermOnline

YBR253W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR009332. Mediator_Med22.
IPR016530. Mediator_Med22_Saccharomyce.
[Graphical view]

Pfam

PF06179. Med22. 1 hit.
[Graphical view]

PIRSF

PIRSF007936. SRB6. 1 hit.

ProtoNet

Search...

Other

NextBio

971652.

Entry information

Entry name

MED22_YEAST

Accession

Primary (citable) accession number: P32570
Secondary accession number(s): D6VQP9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program