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Protein

Mediator of RNA polymerase II transcription subunit 17

Gene

SRB4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.5 Publications

GO - Molecular functioni

  • activating transcription factor binding Source: SGD
  • RNA polymerase II core promoter sequence-specific DNA binding Source: SGD
  • RNA polymerase II transcription cofactor activity Source: InterPro
  • transcription factor activity, core RNA polymerase II recruiting Source: SGD

GO - Biological processi

  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • transcription initiation from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-30206-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 17
Alternative name(s):
Mediator complex subunit 17
Suppressor of RNA polymerase B 4
Gene namesi
Name:SRB4
Synonyms:MED17
Ordered Locus Names:YER022W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER022W.
SGDiS000000824. SRB4.

Subcellular locationi

GO - Cellular componenti

  • core mediator complex Source: SGD
  • mediator complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi353G → C in SRB4-1; suppresses the phenotypic defects of an RNA polymerase II CTD truncation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000963661 – 687Mediator of RNA polymerase II transcription subunit 17Add BLAST687

Proteomic databases

MaxQBiP32569.
PRIDEiP32569.

PTM databases

iPTMnetiP32569.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. The head module may also interact with the TFIIF complex. SRB4/MED17 interacts directly with MED6, MED11, ROX3/MED19, SRB2/MED20 and SRB6/MED22. Interacts directly with the activator GAL4.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MED6P387823EBI-18025,EBI-10667
MED8P383045EBI-18025,EBI-20932
SOH1P386334EBI-18025,EBI-17658
SRB6P325705EBI-18025,EBI-18039

GO - Molecular functioni

  • activating transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi36756. 100 interactors.
DIPiDIP-152N.
IntActiP32569. 27 interactors.
MINTiMINT-473310.

Structurei

Secondary structure

1687
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi388 – 396Combined sources9
Beta strandi403 – 408Combined sources6
Helixi425 – 449Combined sources25
Helixi450 – 455Combined sources6
Beta strandi457 – 461Combined sources5
Beta strandi464 – 468Combined sources5
Beta strandi470 – 479Combined sources10
Helixi498 – 524Combined sources27
Turni525 – 527Combined sources3
Helixi535 – 538Combined sources4
Helixi542 – 565Combined sources24
Turni566 – 569Combined sources4
Beta strandi574 – 579Combined sources6
Helixi597 – 604Combined sources8
Helixi607 – 610Combined sources4
Beta strandi614 – 620Combined sources7
Beta strandi630 – 636Combined sources7
Beta strandi640 – 642Combined sources3
Beta strandi644 – 650Combined sources7
Turni654 – 656Combined sources3
Beta strandi660 – 665Combined sources6
Helixi667 – 680Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J1Oelectron microscopy16.00I197-616[»]
I669-687[»]
3RJ1X-ray4.30B/I/P109-616[»]
B/I/P669-687[»]
4GWPX-ray4.20B1-687[»]
4GWQX-ray4.50B1-687[»]
4H62X-ray3.00Q377-687[»]
4V1Oelectron microscopy9.70W2-687[»]
5SVAelectron microscopy15.30P1-687[»]
ProteinModelPortaliP32569.
SMRiP32569.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mediator complex subunit 17 family.Curated

Phylogenomic databases

HOGENOMiHOG000113527.
InParanoidiP32569.
KOiK15134.
OMAiAMNESSL.
OrthoDBiEOG092C3XD1.

Family and domain databases

InterProiIPR019313. Mediator_Med17.
[Graphical view]
PfamiPF10156. Med17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32569-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTEDPDSNH LSSETGIKLA LDPNLITLAL SSNPNSSLHS PTSDEPVPES
60 70 80 90 100
AGKADTSIRL EGDELENKTK KDNDKNLKFL KNKDSLVSNP HEIYGSMPLE
110 120 130 140 150
QLIPIILRQR GPGFKFVDLN EKELQNEIKQ LGSDSSDGHN SEKKDTDGAD
160 170 180 190 200
ENVQIGEDFM EVDYEDKDNP VDSRNETDHK TNENGETDDN IETVMTQEQF
210 220 230 240 250
VKRRRDMLEH INLAMNESSL ALEFVSLLLS SVKESTGMSS MSPFLRKVVK
260 270 280 290 300
PSSLNSDKIP YVAPTKKEYI ELDILNKGWK LQSLNESKDL LRASFNKLSS
310 320 330 340 350
ILQNEHDYWN KIMQSISNKD VIFKIRDRTS GQKLLAIKYG YEDSGSTYKH
360 370 380 390 400
DRGIANIRNN IESQNLDLIP HSSSVFKGTD FVHSVKKFLR VRIFTKIESE
410 420 430 440 450
DDYILSGESV MDRDSESEEA ETKDIRKQIQ LLKKIIFEKE LMYQIKKECA
460 470 480 490 500
LLISYGVSIE NENKVIIELP NEKFEIELLS LDDDSIVNHE QDLPKINDKR
510 520 530 540 550
ANLMLVMLRL LLVVIFKKTL RSRISSPHGL INLNVDDDIL IIRPILGKVR
560 570 580 590 600
FANYKLLLKK IIKDYVLDIV PGSSITETEV EREQPQENKN IDDENITKLN
610 620 630 640 650
KEIRAFDKLL NIPRRELKIN LPLTEHKSPN LSLMLESPNY CNALIHIKFS
660 670 680
AGTEANAVSF DTTFSDFKEV EDFLHFIVAE YIQQKKV
Length:687
Mass (Da):78,476
Last modified:October 1, 1993 - v1
Checksum:iD25B2993A1BADFD6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti430 – 431QL → PI in CAA40613 (PubMed:1508147).Curated2
Sequence conflicti647I → T in AAT93185 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12026 Unassigned DNA. Translation: AAA02632.1.
U18778 Genomic DNA. Translation: AAB64555.1.
AY693166 Genomic DNA. Translation: AAT93185.1.
X57338 Genomic DNA. Translation: CAA40613.1.
BK006939 Genomic DNA. Translation: DAA07675.1.
PIRiA40711.
RefSeqiNP_010939.1. NM_001178913.1.

Genome annotation databases

EnsemblFungiiYER022W; YER022W; YER022W.
GeneIDi856743.
KEGGisce:YER022W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12026 Unassigned DNA. Translation: AAA02632.1.
U18778 Genomic DNA. Translation: AAB64555.1.
AY693166 Genomic DNA. Translation: AAT93185.1.
X57338 Genomic DNA. Translation: CAA40613.1.
BK006939 Genomic DNA. Translation: DAA07675.1.
PIRiA40711.
RefSeqiNP_010939.1. NM_001178913.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J1Oelectron microscopy16.00I197-616[»]
I669-687[»]
3RJ1X-ray4.30B/I/P109-616[»]
B/I/P669-687[»]
4GWPX-ray4.20B1-687[»]
4GWQX-ray4.50B1-687[»]
4H62X-ray3.00Q377-687[»]
4V1Oelectron microscopy9.70W2-687[»]
5SVAelectron microscopy15.30P1-687[»]
ProteinModelPortaliP32569.
SMRiP32569.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36756. 100 interactors.
DIPiDIP-152N.
IntActiP32569. 27 interactors.
MINTiMINT-473310.

PTM databases

iPTMnetiP32569.

Proteomic databases

MaxQBiP32569.
PRIDEiP32569.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER022W; YER022W; YER022W.
GeneIDi856743.
KEGGisce:YER022W.

Organism-specific databases

EuPathDBiFungiDB:YER022W.
SGDiS000000824. SRB4.

Phylogenomic databases

HOGENOMiHOG000113527.
InParanoidiP32569.
KOiK15134.
OMAiAMNESSL.
OrthoDBiEOG092C3XD1.

Enzyme and pathway databases

BioCyciYEAST:G3O-30206-MONOMER.

Miscellaneous databases

PROiP32569.

Family and domain databases

InterProiIPR019313. Mediator_Med17.
[Graphical view]
PfamiPF10156. Med17. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMED17_YEAST
AccessioniPrimary (citable) accession number: P32569
Secondary accession number(s): D3DLS1, Q06790, Q6B1B4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 30, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1720 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.