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P32562 (CDC5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 144. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cell cycle serine/threonine-protein kinase CDC5/MSD2

EC=2.7.11.21
Gene names
Name:CDC5
Synonyms:MSD2, PKX2
Ordered Locus Names:YMR001C
ORF Names:YM8270.03C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length705 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protein kinase required for the cell cycle where it is involved in mitotic exit. A component of the fear (CDC14 early anaphase release) network which promotes CDC14 release from the nucleolus during early anaphase. Phosphorylates SCC1/MCD1 and NET1. Ref.4 Ref.5 Ref.6

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Miscellaneous

Present with 1480 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.

Contains 2 POLO box domains.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Mitosis
   DomainRepeat
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmitotic nuclear division

Inferred from electronic annotation. Source: UniProtKB-KW

positive regulation of catalytic activity

Inferred from direct assay PubMed 16763112. Source: GOC

positive regulation of spindle pole body separation

Inferred from mutant phenotype PubMed 18500339. Source: SGD

protein localization

Inferred from mutant phenotype PubMed 16763112. Source: SGD

protein phosphorylation

Inferred from direct assay PubMed 17122856Ref.1. Source: SGD

resolution of meiotic recombination intermediates

Inferred from mutant phenotype PubMed 12717442. Source: SGD

spindle assembly involved in meiosis

Inferred from mutant phenotype PubMed 20237423. Source: SGD

synaptonemal complex disassembly

Inferred from genetic interaction PubMed 18832066. Source: SGD

   Cellular_componentcellular bud neck

Inferred from direct assay PubMed 10594031. Source: SGD

nucleus

Inferred from direct assay PubMed 9819423. Source: SGD

spindle pole

Inferred from direct assay PubMed 10594031. Source: SGD

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

enzyme activator activity

Inferred from direct assay PubMed 16763112. Source: SGD

protein binding

Inferred from physical interaction PubMed 11438652PubMed 18719252PubMed 18927509PubMed 19013276. Source: IntAct

protein kinase activity

Inferred from direct assay PubMed 12637549PubMed 17122856Ref.1. Source: SGD

protein serine/threonine kinase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 705705Cell cycle serine/threonine-protein kinase CDC5/MSD2
PRO_0000085762

Regions

Domain82 – 337256Protein kinase
Domain520 – 58768POLO box 1
Domain619 – 69274POLO box 2
Nucleotide binding88 – 969ATP By similarity

Sites

Active site2041Proton acceptor By similarity
Binding site1101ATP By similarity

Amino acid modifications

Modified residue231Phosphothreonine Ref.10
Modified residue4191Phosphoserine Ref.8

Sequences

Sequence LengthMass (Da)Tools
P32562 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: B5A25F1BBBEAA3DC

FASTA70581,031
        10         20         30         40         50         60 
MSLGPLKAIN DKQLNTRSKL VHTPIKGNTA DLVGKENHFK QTKRLDPNND HHHQPAQKKK 

        70         80         90        100        110        120 
REKLSALCKT PPSLIKTRGK DYHRGHFLGE GGFARCFQIK DDSGEIFAAK TVAKASIKSE 

       130        140        150        160        170        180 
KTRKKLLSEI QIHKSMSHPN IVQFIDCFED DSNVYILLEI CPNGSLMELL KRRKVLTEPE 

       190        200        210        220        230        240 
VRFFTTQICG AIKYMHSRRV IHRDLKLGNI FFDSNYNLKI GDFGLAAVLA NESERKYTIC 

       250        260        270        280        290        300 
GTPNYIAPEV LMGKHSGHSF EVDIWSLGVM LYALLIGKPP FQARDVNTIY ERIKCRDFSF 

       310        320        330        340        350        360 
PRDKPISDEG KILIRDILSL DPIERPSLTE IMDYVWFRGT FPPSIPSTVM SEAPNFEDIP 

       370        380        390        400        410        420 
EEQSLVNFKD CMEKSLLLES MSSDKIQRQK RDYISSIKSS IDKLEEYHQN RPFLPHSLSP 

       430        440        450        460        470        480 
GGTKQKYKEV VDIEAQRRLN DLAREARIRR AQQAVLRKEL IATSTNVIKS EISLRILASE 

       490        500        510        520        530        540 
CHLTLNGIVE AEAQYKMGGL PKSRLPKIKH PMIVTKWVDY SNKHGFSYQL STEDIGVLFN 

       550        560        570        580        590        600 
NGTTVLRLAD AEEFWYISYD DREGWVASHY LLSEKPRELS RHLEVVDFFA KYMKANLSRV 

       610        620        630        640        650        660 
STFGREEYHK DDVFLRRYTR YKPFVMFELS DGTFQFNFKD HHKMAISDGG KLVTYISPSH 

       670        680        690        700 
ESTTYPLVEV LKYGEIPGYP ESNFREKLTL IKEGLKQKST IVTVD 

« Hide

References

« Hide 'large scale' references
[1]"A multicopy suppressor gene of the Saccharomyces cerevisiae G1 cell cycle mutant gene dbf4 encodes a protein kinase and is identified as CDC5."
Kitada K., Sugino A., Johnston L.H., Johnson A.L.
Mol. Cell. Biol. 13:4445-4457(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: ATCC 204626 / S288c / A364A.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Phosphorylation of the cohesin subunit Scc1 by Polo/Cdc5 kinase regulates sister chromatid separation in yeast."
Alexandru G., Uhlmann F., Mechtler K., Poupart M.-A., Nasmyth K.
Cell 105:459-472(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN PHOSPHORYLATION OF SCC1.
[5]"Budding yeast Cdc5 phosphorylates Net1 and assists Cdc14 release from the nucleolus."
Yoshida S., Toh-e A.
Biochem. Biophys. Res. Commun. 294:687-691(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN PHOSPHORYLATION OF NET1.
[6]"Separase, polo kinase, the kinetochore protein Slk19, and Spo12 function in a network that controls Cdc14 localization during early anaphase."
Stegmeier F., Visintin R., Amon A.
Cell 108:207-220(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A COMPONENT OF THE FEAR NETWORK.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-419, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-23, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M84220 Unassigned DNA. Translation: AAA02576.1.
Z48613 Genomic DNA. Translation: CAA88516.1.
BK006946 Genomic DNA. Translation: DAA09899.1.
PIRA48144.
RefSeqNP_013714.1. NM_001182497.1.

3D structure databases

ProteinModelPortalP32562.
SMRP32562. Positions 69-445, 514-674.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35171. 172 interactions.
DIPDIP-2321N.
IntActP32562. 54 interactions.
MINTMINT-482878.
STRING4932.YMR001C.

Proteomic databases

MaxQBP32562.
PaxDbP32562.
PeptideAtlasP32562.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR001C; YMR001C; YMR001C.
GeneID855013.
KEGGsce:YMR001C.

Organism-specific databases

CYGDYMR001c.
SGDS000004603. CDC5.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00530000062954.
HOGENOMHOG000248546.
KOK06660.
OMALSEIQIH.
OrthoDBEOG793BK1.

Enzyme and pathway databases

BioCycYEAST:G3O-32712-MONOMER.
BRENDA2.7.11.21. 984.

Gene expression databases

GenevestigatorP32562.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000959. POLO_box_duplicated_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
PF00659. POLO_box. 2 hits.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50078. POLO_BOX. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978186.
PROP32562.

Entry information

Entry nameCDC5_YEAST
AccessionPrimary (citable) accession number: P32562
Secondary accession number(s): D6VZH5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 11, 2014
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families