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Protein

Cell cycle serine/threonine-protein kinase CDC5/MSD2

Gene

CDC5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein kinase required for the cell cycle where it is involved in mitotic exit. A component of the fear (CDC14 early anaphase release) network which promotes CDC14 release from the nucleolus during early anaphase. Phosphorylates SCC1/MCD1 and NET1.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei110ATPPROSITE-ProRule annotation1
Active sitei204Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi88 – 96ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • centromeric DNA binding Source: SGD
  • enzyme activator activity Source: SGD
  • phosphoprotein binding Source: SGD
  • protein kinase activity Source: SGD
  • protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • exit from mitosis Source: SGD
  • positive regulation of spindle pole body separation Source: SGD
  • protein localization Source: SGD
  • protein phosphorylation Source: SGD
  • regulation of nucleus organization Source: SGD
  • regulation of protein localization to mitotic spindle pole body Source: SGD
  • resolution of meiotic recombination intermediates Source: SGD
  • spindle assembly involved in meiosis Source: SGD
  • synaptonemal complex disassembly Source: SGD

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Cell division, Mitosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32712-MONOMER.
BRENDAi2.7.11.21. 984.
ReactomeiR-SCE-156711. Polo-like kinase mediated events.
R-SCE-162658. Golgi Cisternae Pericentriolar Stack Reorganization.
R-SCE-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-SCE-2980767. Activation of NIMA Kinases NEK9, NEK6, NEK7.
R-SCE-6804115. TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain.
R-SCE-68884. Mitotic Telophase/Cytokinesis.
R-SCE-69273. Cyclin A/B1 associated events during G2/M transition.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell cycle serine/threonine-protein kinase CDC5/MSD2 (EC:2.7.11.21)
Gene namesi
Name:CDC5
Synonyms:MSD2, PKX2
Ordered Locus Names:YMR001C
ORF Names:YM8270.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR001C.
SGDiS000004603. CDC5.

Subcellular locationi

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • nuclear mitotic cohesin complex Source: SGD
  • nucleus Source: SGD
  • spindle pole Source: SGD

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000857621 – 705Cell cycle serine/threonine-protein kinase CDC5/MSD2Add BLAST705

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei23PhosphothreonineCombined sources1
Modified residuei419PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32562.
PRIDEiP32562.

PTM databases

iPTMnetiP32562.

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

  • phosphoprotein binding Source: SGD

Protein-protein interaction databases

BioGridi35171. 179 interactors.
DIPiDIP-2321N.
IntActiP32562. 54 interactors.
MINTiMINT-482878.

Structurei

3D structure databases

ProteinModelPortaliP32562.
SMRiP32562.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini82 – 337Protein kinasePROSITE-ProRule annotationAdd BLAST256
Domaini520 – 587POLO box 1PROSITE-ProRule annotationAdd BLAST68
Domaini619 – 692POLO box 2PROSITE-ProRule annotationAdd BLAST74

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00530000062954.
HOGENOMiHOG000248546.
InParanoidiP32562.
KOiK06660.
OMAiRRYTRYK.
OrthoDBiEOG092C0QJU.

Family and domain databases

CDDicd13118. POLO_box_1. 1 hit.
cd13117. POLO_box_2. 1 hit.
Gene3Di3.30.1120.30. 2 hits.
InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR033701. POLO_box_1.
IPR033695. POLO_box_2.
IPR000959. POLO_box_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
PF00659. POLO_box. 2 hits.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS50078. POLO_BOX. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

P32562-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLGPLKAIN DKQLNTRSKL VHTPIKGNTA DLVGKENHFK QTKRLDPNND
60 70 80 90 100
HHHQPAQKKK REKLSALCKT PPSLIKTRGK DYHRGHFLGE GGFARCFQIK
110 120 130 140 150
DDSGEIFAAK TVAKASIKSE KTRKKLLSEI QIHKSMSHPN IVQFIDCFED
160 170 180 190 200
DSNVYILLEI CPNGSLMELL KRRKVLTEPE VRFFTTQICG AIKYMHSRRV
210 220 230 240 250
IHRDLKLGNI FFDSNYNLKI GDFGLAAVLA NESERKYTIC GTPNYIAPEV
260 270 280 290 300
LMGKHSGHSF EVDIWSLGVM LYALLIGKPP FQARDVNTIY ERIKCRDFSF
310 320 330 340 350
PRDKPISDEG KILIRDILSL DPIERPSLTE IMDYVWFRGT FPPSIPSTVM
360 370 380 390 400
SEAPNFEDIP EEQSLVNFKD CMEKSLLLES MSSDKIQRQK RDYISSIKSS
410 420 430 440 450
IDKLEEYHQN RPFLPHSLSP GGTKQKYKEV VDIEAQRRLN DLAREARIRR
460 470 480 490 500
AQQAVLRKEL IATSTNVIKS EISLRILASE CHLTLNGIVE AEAQYKMGGL
510 520 530 540 550
PKSRLPKIKH PMIVTKWVDY SNKHGFSYQL STEDIGVLFN NGTTVLRLAD
560 570 580 590 600
AEEFWYISYD DREGWVASHY LLSEKPRELS RHLEVVDFFA KYMKANLSRV
610 620 630 640 650
STFGREEYHK DDVFLRRYTR YKPFVMFELS DGTFQFNFKD HHKMAISDGG
660 670 680 690 700
KLVTYISPSH ESTTYPLVEV LKYGEIPGYP ESNFREKLTL IKEGLKQKST

IVTVD
Length:705
Mass (Da):81,031
Last modified:October 1, 1993 - v1
Checksum:iB5A25F1BBBEAA3DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84220 Unassigned DNA. Translation: AAA02576.1.
Z48613 Genomic DNA. Translation: CAA88516.1.
BK006946 Genomic DNA. Translation: DAA09899.1.
PIRiA48144.
RefSeqiNP_013714.1. NM_001182497.1.

Genome annotation databases

EnsemblFungiiYMR001C; YMR001C; YMR001C.
GeneIDi855013.
KEGGisce:YMR001C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84220 Unassigned DNA. Translation: AAA02576.1.
Z48613 Genomic DNA. Translation: CAA88516.1.
BK006946 Genomic DNA. Translation: DAA09899.1.
PIRiA48144.
RefSeqiNP_013714.1. NM_001182497.1.

3D structure databases

ProteinModelPortaliP32562.
SMRiP32562.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35171. 179 interactors.
DIPiDIP-2321N.
IntActiP32562. 54 interactors.
MINTiMINT-482878.

PTM databases

iPTMnetiP32562.

Proteomic databases

MaxQBiP32562.
PRIDEiP32562.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR001C; YMR001C; YMR001C.
GeneIDi855013.
KEGGisce:YMR001C.

Organism-specific databases

EuPathDBiFungiDB:YMR001C.
SGDiS000004603. CDC5.

Phylogenomic databases

GeneTreeiENSGT00530000062954.
HOGENOMiHOG000248546.
InParanoidiP32562.
KOiK06660.
OMAiRRYTRYK.
OrthoDBiEOG092C0QJU.

Enzyme and pathway databases

BioCyciYEAST:G3O-32712-MONOMER.
BRENDAi2.7.11.21. 984.
ReactomeiR-SCE-156711. Polo-like kinase mediated events.
R-SCE-162658. Golgi Cisternae Pericentriolar Stack Reorganization.
R-SCE-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-SCE-2980767. Activation of NIMA Kinases NEK9, NEK6, NEK7.
R-SCE-6804115. TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain.
R-SCE-68884. Mitotic Telophase/Cytokinesis.
R-SCE-69273. Cyclin A/B1 associated events during G2/M transition.

Miscellaneous databases

PROiP32562.

Family and domain databases

CDDicd13118. POLO_box_1. 1 hit.
cd13117. POLO_box_2. 1 hit.
Gene3Di3.30.1120.30. 2 hits.
InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR033701. POLO_box_1.
IPR033695. POLO_box_2.
IPR000959. POLO_box_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
PF00659. POLO_box. 2 hits.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS50078. POLO_BOX. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCDC5_YEAST
AccessioniPrimary (citable) accession number: P32562
Secondary accession number(s): D6VZH5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: March 15, 2017
This is version 172 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1480 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.