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P32562

- CDC5_YEAST

UniProt

P32562 - CDC5_YEAST

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Protein
Cell cycle serine/threonine-protein kinase CDC5/MSD2
Gene
CDC5, MSD2, PKX2, YMR001C, YM8270.03C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Protein kinase required for the cell cycle where it is involved in mitotic exit. A component of the fear (CDC14 early anaphase release) network which promotes CDC14 release from the nucleolus during early anaphase. Phosphorylates SCC1/MCD1 and NET1.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei110 – 1101ATP By similarity
Active sitei204 – 2041Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi88 – 969ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. enzyme activator activity Source: SGD
  3. protein binding Source: IntAct
  4. protein kinase activity Source: SGD
  5. protein serine/threonine kinase activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. mitotic nuclear division Source: UniProtKB-KW
  2. positive regulation of catalytic activity Source: GOC
  3. positive regulation of spindle pole body separation Source: SGD
  4. protein localization Source: SGD
  5. protein phosphorylation Source: SGD
  6. resolution of meiotic recombination intermediates Source: SGD
  7. spindle assembly involved in meiosis Source: SGD
  8. synaptonemal complex disassembly Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32712-MONOMER.
BRENDAi2.7.11.21. 984.
ReactomeiREACT_188487. Cyclin A/B1 associated events during G2/M transition.
REACT_188776. Regulation of PLK1 Activity at G2/M Transition.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell cycle serine/threonine-protein kinase CDC5/MSD2 (EC:2.7.11.21)
Gene namesi
Name:CDC5
Synonyms:MSD2, PKX2
Ordered Locus Names:YMR001C
ORF Names:YM8270.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR001c.
SGDiS000004603. CDC5.

Subcellular locationi

GO - Cellular componenti

  1. cellular bud neck Source: SGD
  2. nucleus Source: SGD
  3. spindle pole Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 705705Cell cycle serine/threonine-protein kinase CDC5/MSD2
PRO_0000085762Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei23 – 231Phosphothreonine1 Publication
Modified residuei419 – 4191Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32562.
PaxDbiP32562.
PeptideAtlasiP32562.

Expressioni

Gene expression databases

GenevestigatoriP32562.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC14Q006842EBI-4440,EBI-4192
CDC7P0624311EBI-4440,EBI-4451
LRS4Q040876EBI-4440,EBI-32189
SPO13P236248EBI-4440,EBI-17719
SWE1P329444EBI-4440,EBI-18607

Protein-protein interaction databases

BioGridi35171. 172 interactions.
DIPiDIP-2321N.
IntActiP32562. 54 interactions.
MINTiMINT-482878.
STRINGi4932.YMR001C.

Structurei

3D structure databases

ProteinModelPortaliP32562.
SMRiP32562. Positions 69-445, 514-674.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini82 – 337256Protein kinase
Add
BLAST
Domaini520 – 58768POLO box 1
Add
BLAST
Domaini619 – 69274POLO box 2
Add
BLAST

Sequence similaritiesi

Contains 2 POLO box domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00530000062954.
HOGENOMiHOG000248546.
KOiK06660.
OMAiLSEIQIH.
OrthoDBiEOG793BK1.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000959. POLO_box_duplicated_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00659. POLO_box. 2 hits.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50078. POLO_BOX. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32562-1 [UniParc]FASTAAdd to Basket

« Hide

MSLGPLKAIN DKQLNTRSKL VHTPIKGNTA DLVGKENHFK QTKRLDPNND    50
HHHQPAQKKK REKLSALCKT PPSLIKTRGK DYHRGHFLGE GGFARCFQIK 100
DDSGEIFAAK TVAKASIKSE KTRKKLLSEI QIHKSMSHPN IVQFIDCFED 150
DSNVYILLEI CPNGSLMELL KRRKVLTEPE VRFFTTQICG AIKYMHSRRV 200
IHRDLKLGNI FFDSNYNLKI GDFGLAAVLA NESERKYTIC GTPNYIAPEV 250
LMGKHSGHSF EVDIWSLGVM LYALLIGKPP FQARDVNTIY ERIKCRDFSF 300
PRDKPISDEG KILIRDILSL DPIERPSLTE IMDYVWFRGT FPPSIPSTVM 350
SEAPNFEDIP EEQSLVNFKD CMEKSLLLES MSSDKIQRQK RDYISSIKSS 400
IDKLEEYHQN RPFLPHSLSP GGTKQKYKEV VDIEAQRRLN DLAREARIRR 450
AQQAVLRKEL IATSTNVIKS EISLRILASE CHLTLNGIVE AEAQYKMGGL 500
PKSRLPKIKH PMIVTKWVDY SNKHGFSYQL STEDIGVLFN NGTTVLRLAD 550
AEEFWYISYD DREGWVASHY LLSEKPRELS RHLEVVDFFA KYMKANLSRV 600
STFGREEYHK DDVFLRRYTR YKPFVMFELS DGTFQFNFKD HHKMAISDGG 650
KLVTYISPSH ESTTYPLVEV LKYGEIPGYP ESNFREKLTL IKEGLKQKST 700
IVTVD 705
Length:705
Mass (Da):81,031
Last modified:October 1, 1993 - v1
Checksum:iB5A25F1BBBEAA3DC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M84220 Unassigned DNA. Translation: AAA02576.1.
Z48613 Genomic DNA. Translation: CAA88516.1.
BK006946 Genomic DNA. Translation: DAA09899.1.
PIRiA48144.
RefSeqiNP_013714.1. NM_001182497.1.

Genome annotation databases

EnsemblFungiiYMR001C; YMR001C; YMR001C.
GeneIDi855013.
KEGGisce:YMR001C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M84220 Unassigned DNA. Translation: AAA02576.1 .
Z48613 Genomic DNA. Translation: CAA88516.1 .
BK006946 Genomic DNA. Translation: DAA09899.1 .
PIRi A48144.
RefSeqi NP_013714.1. NM_001182497.1.

3D structure databases

ProteinModelPortali P32562.
SMRi P32562. Positions 69-445, 514-674.
ModBasei Search...

Protein-protein interaction databases

BioGridi 35171. 172 interactions.
DIPi DIP-2321N.
IntActi P32562. 54 interactions.
MINTi MINT-482878.
STRINGi 4932.YMR001C.

Proteomic databases

MaxQBi P32562.
PaxDbi P32562.
PeptideAtlasi P32562.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR001C ; YMR001C ; YMR001C .
GeneIDi 855013.
KEGGi sce:YMR001C.

Organism-specific databases

CYGDi YMR001c.
SGDi S000004603. CDC5.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00530000062954.
HOGENOMi HOG000248546.
KOi K06660.
OMAi LSEIQIH.
OrthoDBi EOG793BK1.

Enzyme and pathway databases

BioCyci YEAST:G3O-32712-MONOMER.
BRENDAi 2.7.11.21. 984.
Reactomei REACT_188487. Cyclin A/B1 associated events during G2/M transition.
REACT_188776. Regulation of PLK1 Activity at G2/M Transition.

Miscellaneous databases

NextBioi 978186.
PROi P32562.

Gene expression databases

Genevestigatori P32562.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000959. POLO_box_duplicated_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
PF00659. POLO_box. 2 hits.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS50078. POLO_BOX. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A multicopy suppressor gene of the Saccharomyces cerevisiae G1 cell cycle mutant gene dbf4 encodes a protein kinase and is identified as CDC5."
    Kitada K., Sugino A., Johnston L.H., Johnson A.L.
    Mol. Cell. Biol. 13:4445-4457(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: ATCC 204626 / S288c / A364A.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Phosphorylation of the cohesin subunit Scc1 by Polo/Cdc5 kinase regulates sister chromatid separation in yeast."
    Alexandru G., Uhlmann F., Mechtler K., Poupart M.-A., Nasmyth K.
    Cell 105:459-472(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN PHOSPHORYLATION OF SCC1.
  5. "Budding yeast Cdc5 phosphorylates Net1 and assists Cdc14 release from the nucleolus."
    Yoshida S., Toh-e A.
    Biochem. Biophys. Res. Commun. 294:687-691(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN PHOSPHORYLATION OF NET1.
  6. "Separase, polo kinase, the kinetochore protein Slk19, and Spo12 function in a network that controls Cdc14 localization during early anaphase."
    Stegmeier F., Visintin R., Amon A.
    Cell 108:207-220(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A COMPONENT OF THE FEAR NETWORK.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-419, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-23, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCDC5_YEAST
AccessioniPrimary (citable) accession number: P32562
Secondary accession number(s): D6VZH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: September 3, 2014
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1480 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

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