Reviewed, UniProtKB/Swiss-Prot P32562 (CDC5_YEAST)

Names and origin

Protein names

Recommended name:
    Cell cycle serine/threonine-protein kinase CDC5/MSD2
    EC=2.7.11.21

Gene names

Name:	CDC5
Synonyms:	MSD2, PKX2
Ordered Locus Names:	YMR001C
ORF Names:	YM8270.03C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	705 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Protein kinase required for the cell cycle where it is involved in mitotic exit. A component of the fear (CDC14 early anaphase release) network which promotes CDC14 release from the nucleolus during early anaphase. Phosphorylates SCC1/MCD1 and NET1. Ref.3 Ref.4 Ref.5
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Miscellaneous	Present with 1480 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily. Contains 2 POLO box domains. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA-dependent DNA replication Inferred from direct assay. Source: SGD cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of spindle pole body separation Inferred from mutant phenotype. Source: SGD protein amino acid phosphorylation Ref.1 Inferred from direct assay. Source: SGD resolution of meiotic joint molecules as recombinants Inferred from mutant phenotype. Source: SGD synaptonemal complex disassembly Inferred from genetic interaction. Source: SGD
Cellular component	cellular bud neck Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD spindle pole Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW identical protein binding Inferred from physical interaction. Source: IntAct polo kinase kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-4440,EBI-4440
CDC14	Q00684	2	EBI-4440,EBI-4192
CDC7	P06243	1	EBI-4440,EBI-4451
ESP1	Q03018	1	EBI-4440,EBI-6657
LRS4	Q04087	1	EBI-4440,EBI-32189
MPS3	P47069	1	EBI-4440,EBI-25811
SLK19	Q08581	1	EBI-4440,EBI-38556
SPO13	P23624	1	EBI-4440,EBI-17719
SSB2	P40150	1	EBI-4440,EBI-8632
SWE1	P32944	3	EBI-4440,EBI-18607

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 705	705	Cell cycle serine/threonine-protein kinase CDC5/MSD2		PRO_0000085762
Regions
	Domain	82 – 337	256	Protein kinase
	Domain	520 – 587	68	POLO box 1
	Domain	619 – 692	74	POLO box 2
	Nucleotide binding	88 – 96	9	ATP By similarity
Sites
	Active site	204	1	Proton acceptor By similarity
	Binding site	110	1	ATP By similarity
Amino acid modifications
	Modified residue	23	1	Phosphothreonine Ref.8
	Modified residue	70	1	Phosphothreonine Ref.8
	Modified residue	73	1	Phosphoserine Ref.8
	Modified residue	238	1	Phosphothreonine Ref.8
	Modified residue	242	1	Phosphothreonine Ref.8
	Modified residue	419	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P32562-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: B5A25F1BBBEAA3DC	FASTA	705	81,031
	10 20 30 40 50 60 MSLGPLKAIN DKQLNTRSKL VHTPIKGNTA DLVGKENHFK QTKRLDPNND HHHQPAQKKK 70 80 90 100 110 120 REKLSALCKT PPSLIKTRGK DYHRGHFLGE GGFARCFQIK DDSGEIFAAK TVAKASIKSE 130 140 150 160 170 180 KTRKKLLSEI QIHKSMSHPN IVQFIDCFED DSNVYILLEI CPNGSLMELL KRRKVLTEPE 190 200 210 220 230 240 VRFFTTQICG AIKYMHSRRV IHRDLKLGNI FFDSNYNLKI GDFGLAAVLA NESERKYTIC 250 260 270 280 290 300 GTPNYIAPEV LMGKHSGHSF EVDIWSLGVM LYALLIGKPP FQARDVNTIY ERIKCRDFSF 310 320 330 340 350 360 PRDKPISDEG KILIRDILSL DPIERPSLTE IMDYVWFRGT FPPSIPSTVM SEAPNFEDIP 370 380 390 400 410 420 EEQSLVNFKD CMEKSLLLES MSSDKIQRQK RDYISSIKSS IDKLEEYHQN RPFLPHSLSP 430 440 450 460 470 480 GGTKQKYKEV VDIEAQRRLN DLAREARIRR AQQAVLRKEL IATSTNVIKS EISLRILASE 490 500 510 520 530 540 CHLTLNGIVE AEAQYKMGGL PKSRLPKIKH PMIVTKWVDY SNKHGFSYQL STEDIGVLFN 550 560 570 580 590 600 NGTTVLRLAD AEEFWYISYD DREGWVASHY LLSEKPRELS RHLEVVDFFA KYMKANLSRV 610 620 630 640 650 660 STFGREEYHK DDVFLRRYTR YKPFVMFELS DGTFQFNFKD HHKMAISDGG KLVTYISPSH 670 680 690 700 ESTTYPLVEV LKYGEIPGYP ESNFREKLTL IKEGLKQKST IVTVD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A multicopy suppressor gene of the Saccharomyces cerevisiae G1 cell cycle mutant gene dbf4 encodes a protein kinase and is identified as CDC5." Kitada K., Sugino A., Johnston L.H., Johnson A.L. Mol. Cell. Biol. 13:4445-4457(1993) [PubMed: 8321244] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 204626 / S288c / A364A.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Phosphorylation of the cohesin subunit Scc1 by Polo/Cdc5 kinase regulates sister chromatid separation in yeast." Alexandru G., Uhlmann F., Mechtler K., Poupart M.-A., Nasmyth K. Cell 105:459-472(2001) [PubMed: 11371343] [Abstract] Cited for: FUNCTION IN PHOSPHORYLATION OF SCC1.
[4]	"Budding yeast Cdc5 phosphorylates Net1 and assists Cdc14 release from the nucleolus." Yoshida S., Toh-e A. Biochem. Biophys. Res. Commun. 294:687-691(2002) [PubMed: 12056824] [Abstract] Cited for: FUNCTION IN PHOSPHORYLATION OF NET1.
[5]	"Separase, polo kinase, the kinetochore protein Slk19, and Spo12 function in a network that controls Cdc14 localization during early anaphase." Stegmeier F., Visintin R., Amon A. Cell 108:207-220(2002) [PubMed: 11832211] [Abstract] Cited for: FUNCTION AS A COMPONENT OF THE FEAR NETWORK.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-419, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-23; THR-70; SER-73; THR-238 AND THR-242, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M84220 Unassigned DNA. Translation: AAA02576.1. Z48613 Genomic DNA. Translation: CAA88516.1.
PIR	A48144.
RefSeq	NP_013714.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2321N.
IntAct	P32562. 58 interactions.
STRING	P32562.
Proteomic databases
PeptideAtlas	P32562.
PRIDE	P32562.
Genome annotation databases
Ensembl	YMR001C; YMR001C; YMR001C; Saccharomyces cerevisiae. [Genome view]
GeneID	855013.
KEGG	sce:YMR001C.
NMPDR	fig|4932.3.peg.4757.
Organism-specific databases
CYGD	YMR001c.
SGD	S000004603. CDC5.
Phylogenomic databases
HOGENOM	P32562.
OMA	LEICPNG
OrthoDB	EOG90ZSDV
Enzyme and pathway databases
BRENDA	2.7.11.21. 250.
Gene expression databases
ArrayExpress	P32562.
Genevestigator	P32562.
GermOnline	YMR001C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_prot_kinase-like. IPR011009. Kinase-like_dom. IPR017450. POLO_box. IPR000959. POLO_box_duplicated. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. IPR015728. Ser/Thr_prot_kinase_Polo-like. [Graphical view]
PANTHER	PTHR22982. Ca/CaM-dep_prot_kinase-like. 1 hit. PTHR22982:SF10. Polo-like_kinase. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. PF00659. POLO_box. 2 hits. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS50078. POLO_BOX. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	978186.

Entry information

Entry name

CDC5_YEAST

Accession

Primary (citable) accession number: P32562

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	November 24, 2009
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)