Cell cycle serine/threonine-protein kinase CDC5/MSD2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P32562 (CDC5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 133. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cell cycle serine/threonine-protein kinase CDC5/MSD2
EC=2.7.11.21

Gene names

Name:	CDC5
Synonyms:	MSD2, PKX2
Ordered Locus Names:	YMR001C
ORF Names:	YM8270.03C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	705 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protein kinase required for the cell cycle where it is involved in mitotic exit. A component of the fear (CDC14 early anaphase release) network which promotes CDC14 release from the nucleolus during early anaphase. Phosphorylates SCC1/MCD1 and NET1. Ref.4 Ref.5 Ref.6
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Miscellaneous	Present with 1480 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily. Contains 2 POLO box domains. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of spindle pole body separation Inferred from mutant phenotype PubMed 18500339. Source: SGD resolution of meiotic recombination intermediates Inferred from mutant phenotype PubMed 12717442. Source: SGD spindle assembly involved in meiosis Inferred from mutant phenotype PubMed 20237423. Source: SGD synaptonemal complex disassembly Inferred from genetic interaction PubMed 18832066. Source: SGD
Cellular_component	cellular bud neck Inferred from direct assay PubMed 10594031. Source: SGD nucleus Inferred from direct assay PubMed 9819423. Source: SGD spindle pole Inferred from direct assay PubMed 10594031. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase activity Inferred from direct assay PubMed 12637549 PubMed 16319894. Source: SGD protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
CDC14	Q00684	2	EBI-4440,EBI-4192
CDC7	P06243	11	EBI-4440,EBI-4451
LRS4	Q04087	6	EBI-4440,EBI-32189
SPO13	P23624	8	EBI-4440,EBI-17719
SWE1	P32944	4	EBI-4440,EBI-18607

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 705

705

Cell cycle serine/threonine-protein kinase CDC5/MSD2

PRO_0000085762

Regions

Domain

82 – 337

256

Protein kinase

Domain

520 – 587

POLO box 1

Domain

619 – 692

POLO box 2

Nucleotide binding

88 – 96

ATP By similarity

Sites

Active site

204

Proton acceptor By similarity

Binding site

110

ATP By similarity

Amino acid modifications

Modified residue

Phosphothreonine Ref.9

Modified residue

Phosphothreonine Ref.9

Modified residue

Phosphoserine Ref.9

Modified residue

238

Phosphothreonine Ref.9

Modified residue

242

Phosphothreonine Ref.9

Modified residue

419

Phosphoserine Ref.8

Sequences

Sequence

Length

Mass (Da)

Tools

P32562 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: B5A25F1BBBEAA3DC
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FASTA

705

81,031

        10         20         30         40         50         60 
MSLGPLKAIN DKQLNTRSKL VHTPIKGNTA DLVGKENHFK QTKRLDPNND HHHQPAQKKK 

        70         80         90        100        110        120 
REKLSALCKT PPSLIKTRGK DYHRGHFLGE GGFARCFQIK DDSGEIFAAK TVAKASIKSE 

       130        140        150        160        170        180 
KTRKKLLSEI QIHKSMSHPN IVQFIDCFED DSNVYILLEI CPNGSLMELL KRRKVLTEPE 

       190        200        210        220        230        240 
VRFFTTQICG AIKYMHSRRV IHRDLKLGNI FFDSNYNLKI GDFGLAAVLA NESERKYTIC 

       250        260        270        280        290        300 
GTPNYIAPEV LMGKHSGHSF EVDIWSLGVM LYALLIGKPP FQARDVNTIY ERIKCRDFSF 

       310        320        330        340        350        360 
PRDKPISDEG KILIRDILSL DPIERPSLTE IMDYVWFRGT FPPSIPSTVM SEAPNFEDIP 

       370        380        390        400        410        420 
EEQSLVNFKD CMEKSLLLES MSSDKIQRQK RDYISSIKSS IDKLEEYHQN RPFLPHSLSP 

       430        440        450        460        470        480 
GGTKQKYKEV VDIEAQRRLN DLAREARIRR AQQAVLRKEL IATSTNVIKS EISLRILASE 

       490        500        510        520        530        540 
CHLTLNGIVE AEAQYKMGGL PKSRLPKIKH PMIVTKWVDY SNKHGFSYQL STEDIGVLFN 

       550        560        570        580        590        600 
NGTTVLRLAD AEEFWYISYD DREGWVASHY LLSEKPRELS RHLEVVDFFA KYMKANLSRV 

       610        620        630        640        650        660 
STFGREEYHK DDVFLRRYTR YKPFVMFELS DGTFQFNFKD HHKMAISDGG KLVTYISPSH 

       670        680        690        700 
ESTTYPLVEV LKYGEIPGYP ESNFREKLTL IKEGLKQKST IVTVD

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A multicopy suppressor gene of the Saccharomyces cerevisiae G1 cell cycle mutant gene dbf4 encodes a protein kinase and is identified as CDC5." Kitada K., Sugino A., Johnston L.H., Johnson A.L. Mol. Cell. Biol. 13:4445-4457(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 204626 / S288c / A364A.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Phosphorylation of the cohesin subunit Scc1 by Polo/Cdc5 kinase regulates sister chromatid separation in yeast." Alexandru G., Uhlmann F., Mechtler K., Poupart M.-A., Nasmyth K. Cell 105:459-472(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN PHOSPHORYLATION OF SCC1.
[5]	"Budding yeast Cdc5 phosphorylates Net1 and assists Cdc14 release from the nucleolus." Yoshida S., Toh-e A. Biochem. Biophys. Res. Commun. 294:687-691(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN PHOSPHORYLATION OF NET1.
[6]	"Separase, polo kinase, the kinetochore protein Slk19, and Spo12 function in a network that controls Cdc14 localization during early anaphase." Stegmeier F., Visintin R., Amon A. Cell 108:207-220(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A COMPONENT OF THE FEAR NETWORK.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-419, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-23; THR-70; SER-73; THR-238 AND THR-242, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M84220 Unassigned DNA. Translation: AAA02576.1. Z48613 Genomic DNA. Translation: CAA88516.1. BK006946 Genomic DNA. Translation: DAA09899.1.
PIR	A48144.
RefSeq	NP_013714.1. NM_001182497.1.
3D structure databases
ProteinModelPortal	P32562.
SMR	P32562. Positions 55-398, 514-674.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2321N.
IntAct	P32562. 57 interactions.
MINT	MINT-482878.
STRING	4932.YMR001C.
Proteomic databases
PaxDb	P32562.
PeptideAtlas	P32562.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR001C; YMR001C; YMR001C.
GeneID	855013.
KEGG	sce:YMR001C.
Organism-specific databases
CYGD	YMR001c.
SGD	S000004603. CDC5.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00530000062954.
HOGENOM	HOG000248546.
KO	K06660.
OMA	EIQIHKS.
OrthoDB	EOG4578FS.
Enzyme and pathway databases
BRENDA	2.7.11.21. 984.
Gene expression databases
Genevestigator	P32562.
GermOnline	YMR001C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000959. POLO_box_duplicated_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. PF00659. POLO_box. 2 hits. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS50078. POLO_BOX. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978186.

Entry information

Entry name

CDC5_YEAST

Accession

Primary (citable) accession number: P32562
Secondary accession number(s): D6VZH5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 1, 2013
This is version 133 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents