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P32562

- CDC5_YEAST

UniProt

P32562 - CDC5_YEAST

Protein

Cell cycle serine/threonine-protein kinase CDC5/MSD2

Gene

CDC5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 146 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Protein kinase required for the cell cycle where it is involved in mitotic exit. A component of the fear (CDC14 early anaphase release) network which promotes CDC14 release from the nucleolus during early anaphase. Phosphorylates SCC1/MCD1 and NET1.3 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei110 – 1101ATPPROSITE-ProRule annotation
    Active sitei204 – 2041Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi88 – 969ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. enzyme activator activity Source: SGD
    3. protein binding Source: IntAct
    4. protein kinase activity Source: SGD
    5. protein serine/threonine kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. mitotic nuclear division Source: UniProtKB-KW
    2. positive regulation of catalytic activity Source: GOC
    3. positive regulation of spindle pole body separation Source: SGD
    4. protein localization Source: SGD
    5. protein phosphorylation Source: SGD
    6. resolution of meiotic recombination intermediates Source: SGD
    7. spindle assembly involved in meiosis Source: SGD
    8. synaptonemal complex disassembly Source: SGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32712-MONOMER.
    BRENDAi2.7.11.21. 984.
    ReactomeiREACT_188487. Cyclin A/B1 associated events during G2/M transition.
    REACT_188776. Regulation of PLK1 Activity at G2/M Transition.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cell cycle serine/threonine-protein kinase CDC5/MSD2 (EC:2.7.11.21)
    Gene namesi
    Name:CDC5
    Synonyms:MSD2, PKX2
    Ordered Locus Names:YMR001C
    ORF Names:YM8270.03C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR001c.
    SGDiS000004603. CDC5.

    Subcellular locationi

    GO - Cellular componenti

    1. cellular bud neck Source: SGD
    2. nucleus Source: SGD
    3. spindle pole Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 705705Cell cycle serine/threonine-protein kinase CDC5/MSD2PRO_0000085762Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei23 – 231Phosphothreonine1 Publication
    Modified residuei419 – 4191Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP32562.
    PaxDbiP32562.
    PeptideAtlasiP32562.

    Expressioni

    Gene expression databases

    GenevestigatoriP32562.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CDC14Q006842EBI-4440,EBI-4192
    CDC7P0624311EBI-4440,EBI-4451
    LRS4Q040876EBI-4440,EBI-32189
    SPO13P236248EBI-4440,EBI-17719
    SWE1P329444EBI-4440,EBI-18607

    Protein-protein interaction databases

    BioGridi35171. 172 interactions.
    DIPiDIP-2321N.
    IntActiP32562. 54 interactions.
    MINTiMINT-482878.
    STRINGi4932.YMR001C.

    Structurei

    3D structure databases

    ProteinModelPortaliP32562.
    SMRiP32562. Positions 69-445, 514-674.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini82 – 337256Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini520 – 58768POLO box 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini619 – 69274POLO box 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.PROSITE-ProRule annotation
    Contains 2 POLO box domains.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00530000062954.
    HOGENOMiHOG000248546.
    KOiK06660.
    OMAiLSEIQIH.
    OrthoDBiEOG793BK1.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000959. POLO_box_duplicated_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    PF00659. POLO_box. 2 hits.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50078. POLO_BOX. 2 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32562-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLGPLKAIN DKQLNTRSKL VHTPIKGNTA DLVGKENHFK QTKRLDPNND    50
    HHHQPAQKKK REKLSALCKT PPSLIKTRGK DYHRGHFLGE GGFARCFQIK 100
    DDSGEIFAAK TVAKASIKSE KTRKKLLSEI QIHKSMSHPN IVQFIDCFED 150
    DSNVYILLEI CPNGSLMELL KRRKVLTEPE VRFFTTQICG AIKYMHSRRV 200
    IHRDLKLGNI FFDSNYNLKI GDFGLAAVLA NESERKYTIC GTPNYIAPEV 250
    LMGKHSGHSF EVDIWSLGVM LYALLIGKPP FQARDVNTIY ERIKCRDFSF 300
    PRDKPISDEG KILIRDILSL DPIERPSLTE IMDYVWFRGT FPPSIPSTVM 350
    SEAPNFEDIP EEQSLVNFKD CMEKSLLLES MSSDKIQRQK RDYISSIKSS 400
    IDKLEEYHQN RPFLPHSLSP GGTKQKYKEV VDIEAQRRLN DLAREARIRR 450
    AQQAVLRKEL IATSTNVIKS EISLRILASE CHLTLNGIVE AEAQYKMGGL 500
    PKSRLPKIKH PMIVTKWVDY SNKHGFSYQL STEDIGVLFN NGTTVLRLAD 550
    AEEFWYISYD DREGWVASHY LLSEKPRELS RHLEVVDFFA KYMKANLSRV 600
    STFGREEYHK DDVFLRRYTR YKPFVMFELS DGTFQFNFKD HHKMAISDGG 650
    KLVTYISPSH ESTTYPLVEV LKYGEIPGYP ESNFREKLTL IKEGLKQKST 700
    IVTVD 705
    Length:705
    Mass (Da):81,031
    Last modified:October 1, 1993 - v1
    Checksum:iB5A25F1BBBEAA3DC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84220 Unassigned DNA. Translation: AAA02576.1.
    Z48613 Genomic DNA. Translation: CAA88516.1.
    BK006946 Genomic DNA. Translation: DAA09899.1.
    PIRiA48144.
    RefSeqiNP_013714.1. NM_001182497.1.

    Genome annotation databases

    EnsemblFungiiYMR001C; YMR001C; YMR001C.
    GeneIDi855013.
    KEGGisce:YMR001C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84220 Unassigned DNA. Translation: AAA02576.1 .
    Z48613 Genomic DNA. Translation: CAA88516.1 .
    BK006946 Genomic DNA. Translation: DAA09899.1 .
    PIRi A48144.
    RefSeqi NP_013714.1. NM_001182497.1.

    3D structure databases

    ProteinModelPortali P32562.
    SMRi P32562. Positions 69-445, 514-674.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35171. 172 interactions.
    DIPi DIP-2321N.
    IntActi P32562. 54 interactions.
    MINTi MINT-482878.
    STRINGi 4932.YMR001C.

    Proteomic databases

    MaxQBi P32562.
    PaxDbi P32562.
    PeptideAtlasi P32562.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR001C ; YMR001C ; YMR001C .
    GeneIDi 855013.
    KEGGi sce:YMR001C.

    Organism-specific databases

    CYGDi YMR001c.
    SGDi S000004603. CDC5.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00530000062954.
    HOGENOMi HOG000248546.
    KOi K06660.
    OMAi LSEIQIH.
    OrthoDBi EOG793BK1.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32712-MONOMER.
    BRENDAi 2.7.11.21. 984.
    Reactomei REACT_188487. Cyclin A/B1 associated events during G2/M transition.
    REACT_188776. Regulation of PLK1 Activity at G2/M Transition.

    Miscellaneous databases

    NextBioi 978186.
    PROi P32562.

    Gene expression databases

    Genevestigatori P32562.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000959. POLO_box_duplicated_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    PF00659. POLO_box. 2 hits.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50078. POLO_BOX. 2 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A multicopy suppressor gene of the Saccharomyces cerevisiae G1 cell cycle mutant gene dbf4 encodes a protein kinase and is identified as CDC5."
      Kitada K., Sugino A., Johnston L.H., Johnson A.L.
      Mol. Cell. Biol. 13:4445-4457(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: ATCC 204626 / S288c / A364A.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Phosphorylation of the cohesin subunit Scc1 by Polo/Cdc5 kinase regulates sister chromatid separation in yeast."
      Alexandru G., Uhlmann F., Mechtler K., Poupart M.-A., Nasmyth K.
      Cell 105:459-472(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN PHOSPHORYLATION OF SCC1.
    5. "Budding yeast Cdc5 phosphorylates Net1 and assists Cdc14 release from the nucleolus."
      Yoshida S., Toh-e A.
      Biochem. Biophys. Res. Commun. 294:687-691(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN PHOSPHORYLATION OF NET1.
    6. "Separase, polo kinase, the kinetochore protein Slk19, and Spo12 function in a network that controls Cdc14 localization during early anaphase."
      Stegmeier F., Visintin R., Amon A.
      Cell 108:207-220(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A COMPONENT OF THE FEAR NETWORK.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-419, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-23, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCDC5_YEAST
    AccessioniPrimary (citable) accession number: P32562
    Secondary accession number(s): D6VZH5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 146 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1480 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3