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Protein

Histone deacetylase RPD3

Gene

RPD3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic component of the RPD3 histone deacetylase (HDAC) complexes RPD3C(L) and RPD3C(S) responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation plays an important role in transcriptional regulation, cell cycle progression, DNA damage response, osmotic stress response and developmental events. Is involved in rDNA and telomere silencing and in double strand breaks repair. Required for both full transcription repression and activation of many genes including cell type-specific genes (STE6, TY2 and HO), cell differentiation-specific genes (SPO13), genes that respond to external signals (PHO5) and TRK2. The RPD3 complexes regulate also chromosomal replication timing.19 Publications

Miscellaneous

Present with 3850 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Hydrolysis of an N6-acetyl-lysine residue of a histone to yield a deacetylated histone.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei151Curated1

GO - Molecular functioni

  • histone deacetylase activity Source: SGD
  • NAD-dependent histone deacetylase activity (H3-K14 specific) Source: UniProtKB-EC
  • transcription coactivator activity Source: SGD
  • transcription corepressor activity Source: SGD

GO - Biological processi

  • histone H3 deacetylation Source: SGD
  • histone H4 deacetylation Source: SGD
  • negative regulation of chromatin silencing at rDNA Source: SGD
  • negative regulation of chromatin silencing at silent mating-type cassette Source: SGD
  • negative regulation of chromatin silencing at telomere Source: SGD
  • negative regulation of reciprocal meiotic recombination Source: SGD
  • negative regulation of transcription by RNA polymerase I Source: SGD
  • negative regulation of transcription by RNA polymerase II Source: SGD
  • negative regulation of transcription involved in meiotic cell cycle Source: SGD
  • positive regulation of macroautophagy Source: SGD
  • positive regulation of transcription by RNA polymerase II Source: SGD
  • protein localization to nucleolar rDNA repeats Source: SGD
  • regulation of DNA-dependent DNA replication initiation Source: SGD
  • regulation of transcription involved in G1/S transition of mitotic cell cycle Source: SGD
  • regulation of transcription involved in G2/M transition of mitotic cell cycle Source: SGD
  • replicative cell aging Source: SGD
  • transcription elongation from RNA polymerase II promoter Source: SGD
  • transfer RNA gene-mediated silencing Source: SGD

Keywordsi

Molecular functionChromatin regulator, Hydrolase, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-33314-MONOMER
ReactomeiR-SCE-3214815 HDACs deacetylate histones
R-SCE-4551638 SUMOylation of chromatin organization proteins
R-SCE-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function

Names & Taxonomyi

Protein namesi
Recommended name:
Histone deacetylase RPD3 (EC:3.5.1.98)
Alternative name(s):
Transcriptional regulatory protein RPD3
Gene namesi
Name:RPD3
Synonyms:MOF6, REC3, SDI2, SDS6
Ordered Locus Names:YNL330C
ORF Names:N0305
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL330C
SGDiS000005274 RPD3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi150H → A: Impairs histone deacetylase activity and transcription repression. 1 Publication1
Mutagenesisi151H → A: Impairs histone deacetylase activity and transcription repression. 1 Publication1
Mutagenesisi188H → A: Impairs histone deacetylase activity and transcription repression. 1 Publication1
Mutagenesisi322W → A: Reduces strongly HDAC activity. 1 Publication1
Mutagenesisi325E → A: Reduces strongly HDAC activity. 1 Publication1
Mutagenesisi327G → A: Reduces strongly HDAC activity. 1 Publication1
Mutagenesisi328L → A: Reduces strongly HDAC activity. 1 Publication1
Mutagenesisi329L → A: Reduces strongly HDAC activity. 1 Publication1
Mutagenesisi332V → A: Reduces strongly HDAC activity. 1 Publication1
Mutagenesisi334L → A: Reduces strongly HDAC activity. 1 Publication1
Mutagenesisi335D → A: Reduces strongly HDAC activity. 1 Publication1
Mutagenesisi338L → A: Reduces strongly HDAC activity. 1 Publication1
Mutagenesisi339P → A: Reduces strongly HDAC activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001147241 – 433Histone deacetylase RPD3Add BLAST433

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei394PhosphothreonineCombined sources1
Modified residuei408PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32561
PaxDbiP32561
PRIDEiP32561

PTM databases

iPTMnetiP32561

Interactioni

Subunit structurei

Component of the RPD3C(L) complex composed of at least ASH1, CTI6, DEP1, PHO23, RPD3, RXT2, RXT3, SAP30, SDS3, SIN3, UME1 and UME6. Component of the RPD3C(S) complex composed of at least EAF3, RCO1, RPD3, SIN3, and UME1. Interacts with cyclophilins CPR1, CPR6 and CPR7, with the kinase HOG1, and with ESS1, CYC8 and HAC1.10 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35511, 1031 interactors
DIPiDIP-681N
IntActiP32561, 78 interactors
MINTiP32561
STRINGi4932.YNL330C

Structurei

3D structure databases

ProteinModelPortaliP32561
SMRiP32561
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 331Histone deacetylaseAdd BLAST313

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi320 – 340ESA1-RPD3 motifAdd BLAST21

Domaini

The ESA1-RPD3 (ER) motif is common to ESA1 and RPD3 and is required for ESA1 histone acetyl-transferase (HAT) activity and RPD3 histone deacetylase (HDAC) activity.1 Publication

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00910000144047
HOGENOMiHOG000225180
InParanoidiP32561
KOiK06067
OMAiKRVCYFF
OrthoDBiEOG092C1END

Family and domain databases

Gene3Di3.40.800.20, 1 hit
InterProiView protein in InterPro
IPR000286 His_deacetylse
IPR003084 His_deacetylse_1
IPR023801 His_deacetylse_dom
IPR037138 His_deacetylse_dom_sf
IPR023696 Ureohydrolase_dom_sf
PANTHERiPTHR10625 PTHR10625, 1 hit
PfamiView protein in Pfam
PF00850 Hist_deacetyl, 1 hit
PIRSFiPIRSF037913 His_deacetylse_1, 1 hit
PRINTSiPR01270 HDASUPER
PR01271 HISDACETLASE
SUPFAMiSSF52768 SSF52768, 1 hit

Sequencei

Sequence statusi: Complete.

P32561-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVYEATPFDP ITVKPSDKRR VAYFYDADVG NYAYGAGHPM KPHRIRMAHS
60 70 80 90 100
LIMNYGLYKK MEIYRAKPAT KQEMCQFHTD EYIDFLSRVT PDNLEMFKRE
110 120 130 140 150
SVKFNVGDDC PVFDGLYEYC SISGGGSMEG AARLNRGKCD VAVNYAGGLH
160 170 180 190 200
HAKKSEASGF CYLNDIVLGI IELLRYHPRV LYIDIDVHHG DGVEEAFYTT
210 220 230 240 250
DRVMTCSFHK YGEFFPGTGE LRDIGVGAGK NYAVNVPLRD GIDDATYRSV
260 270 280 290 300
FEPVIKKIME WYQPSAVVLQ CGGDSLSGDR LGCFNLSMEG HANCVNYVKS
310 320 330 340 350
FGIPMMVVGG GGYTMRNVAR TWCFETGLLN NVVLDKDLPY NEYYEYYGPD
360 370 380 390 400
YKLSVRPSNM FNVNTPEYLD KVMTNIFANL ENTKYAPSVQ LNHTPRDAED
410 420 430
LGDVEEDSAE AKDTKGGSQY ARDLHVEHDN EFY
Length:433
Mass (Da):48,904
Last modified:October 1, 1993 - v1
Checksum:i34FFD72A7E7425DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S66438 Genomic DNA Translation: AAB20328.1
X83226 Genomic DNA Translation: CAA58228.1
Z46259 Genomic DNA Translation: CAA86368.1
Z71605 Genomic DNA Translation: CAA96262.1
Z71606 Genomic DNA Translation: CAA96263.1
AY692813 Genomic DNA Translation: AAT92832.1
BK006947 Genomic DNA Translation: DAA10233.1
PIRiS22284
RefSeqiNP_014069.1, NM_001183168.1

Genome annotation databases

EnsemblFungiiYNL330C; YNL330C; YNL330C
GeneIDi855386
KEGGisce:YNL330C

Similar proteinsi

Entry informationi

Entry nameiRPD3_YEAST
AccessioniPrimary (citable) accession number: P32561
Secondary accession number(s): D6W0L7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 182 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

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