Reviewed, UniProtKB/Swiss-Prot P32559 (MSS1_YEAST)

Names and origin

Protein names

Recommended name:
    tRNA modification GTPase MSS1, mitochondrial

Gene names

Name:	MSS1
Synonyms:	PET53
Ordered Locus Names:	YMR023C
ORF Names:	YM9711.13C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	GTPase involved in the 5-carboxymethylaminomethyl modification (mnm5s2U34) of the wobble uridine base in mitochondrial tRNAs. Involved in the expression of cytochrome c oxidase subunit 1 (COX1). Works in association with the small subunit of mitoribosomes. Ref.7
Subunit structure	Forms an heterodimer with MTO1.
Subcellular location	Mitochondrion. Ref.5
Miscellaneous	Present with 768 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the era/mnmE GTP-binding protein family. MnmE subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	GTP-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro tRNA modification Ref.7 Inferred from mutant phenotype. Source: SGD translation Ref.1 Ref.7 Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrial inner membrane Inferred from direct assay. Source: SGD
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 19	19	Mitochondrion Potential
	Chain	20 – 526	507	tRNA modification GTPase MSS1, mitochondrial		PRO_0000035781
Regions
	Nucleotide binding	281 – 288	8	GTP Potential
	Nucleotide binding	328 – 332	5	GTP Potential
	Nucleotide binding	394 – 397	4	GTP Potential
Experimental info
	Sequence conflict	53	1	A → R in CAA49238. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32559-1 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 8740A374CA1E637D	FASTA	526	58,352
	10 20 30 40 50 60 MNSASFLQSR LISRSFLVRR SLKRYSGLAK PYTFQQPTIY ALSTPANQTS AIAIIRISGT 70 80 90 100 110 120 HAKYIYNRLV DSSTVPPIRK AILRNIYSPS SCSVKPHDQK ESKILLDTSL LLYFQAPYSF 130 140 150 160 170 180 TGEDVLELHV HGGKAVVNSI LKAIGSLHDR SSGKDIRFAL PGDFSRRAFQ NGKFDLTQLE 190 200 210 220 230 240 GIKDLIDSET ESQRRSALSS FNGDNKILFE NWRETIIENM AQLTAIIDFA DDNSQEIQNT 250 260 270 280 290 300 DEIFHNVEKN IICLRDQIVT FMQKVEKSTI LQNGIKLVLL GAPNVGKSSL VNSLTNDDIS 310 320 330 340 350 360 IVSDIPGTTR DSIDAMINVN GYKVIICDTA GIREKSSDKI EMLGIDRAKK KSVQSDLCLF 370 380 390 400 410 420 IVDPTDLSKL LPEDILAHLS SKTFGNKRII IVVNKSDLVS DDEMTKVLNK LQTRLGSKYP 430 440 450 460 470 480 ILSVSCKTKE GIESLISTLT SNFESLSQSS ADASPVIVSK RVSEILKNDV LYGLEEFFKS 490 500 510 520 KDFHNDIVLA TENLRYASDG IAKITGQAIG IEEILDSVFS KFCIGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MSS1, a nuclear-encoded mitochondrial GTPase involved in the expression of COX1 subunit of cytochrome c oxidase." Decoster E., Vassal A., Faye G. J. Mol. Biol. 232:79-88(1993) [PubMed: 8392589] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"MSS1, a mitochondrial GTP-binding protein involved in mitochondrial tRNA modification." Li X., Wang X., Guan M.-X. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	"MTO1 codes for a mitochondrial protein required for respiration in paromomycin-resistant mutants of Saccharomyces cerevisiae." Colby G., Wu M., Tzagoloff A. J. Biol. Chem. 273:27945-27952(1998) [PubMed: 9774408] [Abstract] Cited for: INTERACTION WITH MTO1.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Mitochondria-specific RNA-modifying enzymes responsible for the biosynthesis of the wobble base in mitochondrial tRNAs. Implications for the molecular pathogenesis of human mitochondrial diseases." Umeda N., Suzuki T., Yukawa M., Ohya Y., Shindo H., Watanabe K., Suzuki T. J. Biol. Chem. 280:1613-1624(2005) [PubMed: 15509579] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X69481 Genomic DNA. Translation: CAA49238.1. DQ834923 Genomic DNA. Translation: ABG77646.1. Z49211 Genomic DNA. Translation: CAA89126.1.
PIR	S54025.
RefSeq	NP_013736.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5882N.
Genome annotation databases
Ensembl	YMR023C. Saccharomyces cerevisiae. [Contig view]
GeneID	855037.
GenomeReviews	Gene locus YMR023C in contig Z71257_GR.
KEGG	sce:YMR023C.
NMPDR	fig|4932.3.peg.4782.
Organism-specific databases
CYGD	YMR023c.
SGD	S000004625. MSS1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P32559.
OMA	P32559. LIDNAMV.
Gene expression databases
GermOnline	YMR023C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018948. GTP_bd_TrmE_N. IPR002917. MMR_HSR1_GTP_bd. IPR001806. Ras_GTPase. IPR005225. Small_GTP_bd. IPR004520. ThdF. [Graphical view]
Gene3D	G3DSA:3.30.1360.120. GTP_bd_TrmE_N. 1 hit.
Pfam	PF01926. MMR_HSR1. 1 hit. PF10396. TrmE_N. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
TIGRFAMs	TIGR00450. mnmE_trmE_thdF. 1 hit. TIGR00231. small_GTP. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	978251.

Entry information

Entry name

MSS1_YEAST

Accession

Primary (citable) accession number: P32559
Secondary accession number(s): Q0PHA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)