P32559 (MSS1_YEAST) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 109.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: tRNA modification GTPase MSS1, mitochondrial | ||||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome] | ||||||||
| Taxonomic identifier | 559292 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › ![]() |
Protein attributes
| Sequence length | 526 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | GTPase involved in the 5-carboxymethylaminomethyl modification (mnm5s2U34) of the wobble uridine base in mitochondrial tRNAs. Involved in the expression of cytochrome c oxidase subunit 1 (COX1). Works in association with the small subunit of mitoribosomes. Ref.8 |
| Subunit structure | Forms a heterodimer with MTO1. |
| Subcellular location | |
| Miscellaneous | Present with 768 molecules/cell in log phase SD medium. |
| Sequence similarities | Belongs to the Era/MnmE GTP-binding protein family. MnmE subfamily. Contains 1 G (guanine nucleotide-binding) domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | tRNA processing |
| Cellular component | Mitochondrion |
| Domain | Transit peptide |
| Ligand | GTP-binding Nucleotide-binding |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | mitochondrial tRNA wobble uridine modification Inferred from mutant phenotype Ref.8. Source: SGD small GTPase mediated signal transductionInferred from electronic annotation. Source: InterPro |
| Cellular_component | mitochondrial inner membrane Inferred from direct assay PubMed 11604502. Source: SGD |
| Molecular_function | GTP binding Inferred from sequence or structural similarity Ref.1. Source: SGD GTPase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 19 | 19 | Mitochondrion Potential | ||||||
| Chain | 20 – 526 | 507 | tRNA modification GTPase MSS1, mitochondrial | PRO_0000035781 | |||||
Regions | |||||||||
| Domain | 286 – 395 | 110 | G | ||||||
| Nucleotide binding | 281 – 288 | 8 | GTP Potential | ||||||
| Nucleotide binding | 328 – 332 | 5 | GTP Potential | ||||||
| Nucleotide binding | 394 – 397 | 4 | GTP Potential | ||||||
Experimental info | |||||||||
| Sequence conflict | 53 | 1 | A → R in CAA49238. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "MSS1, a nuclear-encoded mitochondrial GTPase involved in the expression of COX1 subunit of cytochrome c oxidase." Decoster E., Vassal A., Faye G. J. Mol. Biol. 232:79-88(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "MSS1, a mitochondrial GTP-binding protein involved in mitochondrial tRNA modification." Li X., Wang X., Guan M.-X. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G.Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [4] | Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c. |
| [5] | "MTO1 codes for a mitochondrial protein required for respiration in paromomycin-resistant mutants of Saccharomyces cerevisiae." Colby G., Wu M., Tzagoloff A. J. Biol. Chem. 273:27945-27952(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MTO1. |
| [6] | "Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [7] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [8] | "Mitochondria-specific RNA-modifying enzymes responsible for the biosynthesis of the wobble base in mitochondrial tRNAs. Implications for the molecular pathogenesis of human mitochondrial diseases." Umeda N., Suzuki T., Yukawa M., Ohya Y., Shindo H., Watanabe K., Suzuki T. J. Biol. Chem. 280:1613-1624(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X69481 Genomic DNA. Translation: CAA49238.1. DQ834923 Genomic DNA. Translation: ABG77646.1. Z49211 Genomic DNA. Translation: CAA89126.1. BK006946 Genomic DNA. Translation: DAA09921.1. |
| PIR | S54025. |
| RefSeq | NP_013736.1. NM_001182519.1. |
3D structure databases | |
| ProteinModelPortal | P32559. |
| SMR | P32559. Positions 38-526. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-5882N. |
| IntAct | P32559. 2 interactions. |
| MINT | MINT-2788166. |
| STRING | 4932.YMR023C. |
Proteomic databases | |
| PaxDb | P32559. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblFungi | YMR023C; YMR023C; YMR023C. |
| GeneID | 855037. |
| KEGG | sce:YMR023C. |
Organism-specific databases | |
| CYGD | YMR023c. |
| SGD | S000004625. MSS1. |
Phylogenomic databases | |
| eggNOG | COG0486. |
| GeneTree | ENSGT00390000016851. |
| HOGENOM | HOG000200714. |
| KO | K03650. |
| OMA | IRISGTH. |
| OrthoDB | EOG4B2X62. |
Gene expression databases | |
| Genevestigator | P32559. |
| GermOnline | YMR023C. Saccharomyces cerevisiae. |
Family and domain databases | |
| Gene3D | 3.30.1360.120. 1 hit. |
| InterPro | IPR018948. GTP-bd_TrmE_N. IPR006073. GTP_binding_domain. IPR004520. GTPase_MnmE. IPR025867. GTPase_MnmE_C_dom. IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR027266. TrmE/GcvT_dom1. [Graphical view] |
| Pfam | PF12631. GTPase_Cys_C. 1 hit. PF01926. MMR_HSR1. 1 hit. PF10396. TrmE_N. 1 hit. [Graphical view] |
| PRINTS | PR00449. RASTRNSFRMNG. |
| TIGRFAMs | TIGR00450. mnmE_trmE_thdF. 1 hit. TIGR00231. small_GTP. 1 hit. |
| ProtoNet | Search... |
Other | |
| NextBio | 978251. |
Entry information
| Entry name | MSS1_YEAST | ||||||||
| Accession | Primary (citable) accession number: P32559 Secondary accession number(s): D6VZJ7, Q0PHA7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Recent format changes Overview of recent format changes |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome XIII Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with
