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Protein

tRNA modification GTPase MSS1, mitochondrial

Gene

MSS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GTPase involved in the 5-carboxymethylaminomethyl modification (mnm5s2U34) of the wobble uridine base in mitochondrial tRNAs. Involved in the expression of cytochrome c oxidase subunit 1 (COX1). Works in association with the small subunit of mitoribosomes.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi281 – 288GTPSequence analysis8
Nucleotide bindingi328 – 332GTPSequence analysis5
Nucleotide bindingi394 – 397GTPSequence analysis4

GO - Molecular functioni

  • GTPase activity Source: GO_Central
  • GTP binding Source: SGD
  • transferase activity Source: Reactome

GO - Biological processi

  • mitochondrial tRNA wobble uridine modification Source: SGD
  • tRNA methylation Source: GO_Central
  • tRNA modification Source: Reactome
Complete GO annotation...

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:G3O-32728-MONOMER.
YEAST:G3O-32728-MONOMER.
ReactomeiR-SCE-6787450. tRNA modification in the mitochondrion.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA modification GTPase MSS1, mitochondrial
Gene namesi
Name:MSS1
Synonyms:PET53
Ordered Locus Names:YMR023C
ORF Names:YM9711.13C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR023C.
SGDiS000004625. MSS1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial inner membrane Source: SGD
  • mitochondrial matrix Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 19MitochondrionSequence analysisAdd BLAST19
ChainiPRO_000003578120 – 526tRNA modification GTPase MSS1, mitochondrialAdd BLAST507

Proteomic databases

MaxQBiP32559.
PRIDEiP32559.

Interactioni

Subunit structurei

Forms a heterodimer with MTO1.

Protein-protein interaction databases

BioGridi35194. 156 interactors.
DIPiDIP-5882N.
IntActiP32559. 2 interactors.
MINTiMINT-2788166.

Structurei

3D structure databases

ProteinModelPortaliP32559.
SMRiP32559.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini274 – 444TrmE-type GAdd BLAST171

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000016851.
HOGENOMiHOG000200714.
InParanoidiP32559.
KOiK03650.
OMAiVIFEKFC.
OrthoDBiEOG092C3WT7.

Family and domain databases

CDDicd04164. trmE. 1 hit.
Gene3Di1.20.120.430. 3 hits.
3.30.1360.120. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00379. GTPase_MnmE. 1 hit.
InterProiIPR031168. G_TrmE.
IPR018948. GTP-bd_TrmE_N.
IPR006073. GTP_binding_domain.
IPR004520. GTPase_MnmE.
IPR027368. MnmE_dom2.
IPR025867. MnmE_helical.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF01926. MMR_HSR1. 1 hit.
PF12631. MnmE_helical. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00450. mnmE_trmE_thdF. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51709. G_TRME. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32559-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSASFLQSR LISRSFLVRR SLKRYSGLAK PYTFQQPTIY ALSTPANQTS
60 70 80 90 100
AIAIIRISGT HAKYIYNRLV DSSTVPPIRK AILRNIYSPS SCSVKPHDQK
110 120 130 140 150
ESKILLDTSL LLYFQAPYSF TGEDVLELHV HGGKAVVNSI LKAIGSLHDR
160 170 180 190 200
SSGKDIRFAL PGDFSRRAFQ NGKFDLTQLE GIKDLIDSET ESQRRSALSS
210 220 230 240 250
FNGDNKILFE NWRETIIENM AQLTAIIDFA DDNSQEIQNT DEIFHNVEKN
260 270 280 290 300
IICLRDQIVT FMQKVEKSTI LQNGIKLVLL GAPNVGKSSL VNSLTNDDIS
310 320 330 340 350
IVSDIPGTTR DSIDAMINVN GYKVIICDTA GIREKSSDKI EMLGIDRAKK
360 370 380 390 400
KSVQSDLCLF IVDPTDLSKL LPEDILAHLS SKTFGNKRII IVVNKSDLVS
410 420 430 440 450
DDEMTKVLNK LQTRLGSKYP ILSVSCKTKE GIESLISTLT SNFESLSQSS
460 470 480 490 500
ADASPVIVSK RVSEILKNDV LYGLEEFFKS KDFHNDIVLA TENLRYASDG
510 520
IAKITGQAIG IEEILDSVFS KFCIGK
Length:526
Mass (Da):58,352
Last modified:October 1, 1996 - v2
Checksum:i8740A374CA1E637D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53A → R in CAA49238 (PubMed:8392589).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69481 Genomic DNA. Translation: CAA49238.1.
DQ834923 Genomic DNA. Translation: ABG77646.1.
Z49211 Genomic DNA. Translation: CAA89126.1.
BK006946 Genomic DNA. Translation: DAA09921.1.
PIRiS54025.
RefSeqiNP_013736.1. NM_001182519.1.

Genome annotation databases

EnsemblFungiiYMR023C; YMR023C; YMR023C.
GeneIDi855037.
KEGGisce:YMR023C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69481 Genomic DNA. Translation: CAA49238.1.
DQ834923 Genomic DNA. Translation: ABG77646.1.
Z49211 Genomic DNA. Translation: CAA89126.1.
BK006946 Genomic DNA. Translation: DAA09921.1.
PIRiS54025.
RefSeqiNP_013736.1. NM_001182519.1.

3D structure databases

ProteinModelPortaliP32559.
SMRiP32559.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35194. 156 interactors.
DIPiDIP-5882N.
IntActiP32559. 2 interactors.
MINTiMINT-2788166.

Proteomic databases

MaxQBiP32559.
PRIDEiP32559.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR023C; YMR023C; YMR023C.
GeneIDi855037.
KEGGisce:YMR023C.

Organism-specific databases

EuPathDBiFungiDB:YMR023C.
SGDiS000004625. MSS1.

Phylogenomic databases

GeneTreeiENSGT00390000016851.
HOGENOMiHOG000200714.
InParanoidiP32559.
KOiK03650.
OMAiVIFEKFC.
OrthoDBiEOG092C3WT7.

Enzyme and pathway databases

BioCyciMetaCyc:G3O-32728-MONOMER.
YEAST:G3O-32728-MONOMER.
ReactomeiR-SCE-6787450. tRNA modification in the mitochondrion.

Miscellaneous databases

PROiP32559.

Family and domain databases

CDDicd04164. trmE. 1 hit.
Gene3Di1.20.120.430. 3 hits.
3.30.1360.120. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00379. GTPase_MnmE. 1 hit.
InterProiIPR031168. G_TrmE.
IPR018948. GTP-bd_TrmE_N.
IPR006073. GTP_binding_domain.
IPR004520. GTPase_MnmE.
IPR027368. MnmE_dom2.
IPR025867. MnmE_helical.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF01926. MMR_HSR1. 1 hit.
PF12631. MnmE_helical. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00450. mnmE_trmE_thdF. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51709. G_TRME. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMSS1_YEAST
AccessioniPrimary (citable) accession number: P32559
Secondary accession number(s): D6VZJ7, Q0PHA7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 768 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.