P32559 (MSS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA modification GTPase MSS1, mitochondrial

Gene names

Name:	MSS1
Synonyms:	PET53
Ordered Locus Names:	YMR023C
ORF Names:	YM9711.13C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	GTPase involved in the 5-carboxymethylaminomethyl modification (mnm5s2U34) of the wobble uridine base in mitochondrial tRNAs. Involved in the expression of cytochrome c oxidase subunit 1 (COX1). Works in association with the small subunit of mitoribosomes. Ref.8
Subunit structure	Forms a heterodimer with MTO1.
Subcellular location	Mitochondrion Ref.6.
Miscellaneous	Present with 768 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the Era/MnmE GTP-binding protein family. MnmE subfamily. Contains 1 G (guanine nucleotide-binding) domain.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	GTP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial tRNA wobble uridine modification Inferred from mutant phenotype Ref.8. Source: SGD small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	mitochondrial inner membrane Inferred from direct assay PubMed 11604502. Source: SGD
Molecular_function	GTP binding Inferred from sequence or structural similarity Ref.1. Source: SGD GTPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 19	19	Mitochondrion Potential
	Chain	20 – 526	507	tRNA modification GTPase MSS1, mitochondrial		PRO_0000035781
Regions
	Domain	286 – 395	110	G
	Nucleotide binding	281 – 288	8	GTP Potential
	Nucleotide binding	328 – 332	5	GTP Potential
	Nucleotide binding	394 – 397	4	GTP Potential
Experimental info
	Sequence conflict	53	1	A → R in CAA49238. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32559 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 8740A374CA1E637D	FASTA	526	58,352
	10 20 30 40 50 60 MNSASFLQSR LISRSFLVRR SLKRYSGLAK PYTFQQPTIY ALSTPANQTS AIAIIRISGT 70 80 90 100 110 120 HAKYIYNRLV DSSTVPPIRK AILRNIYSPS SCSVKPHDQK ESKILLDTSL LLYFQAPYSF 130 140 150 160 170 180 TGEDVLELHV HGGKAVVNSI LKAIGSLHDR SSGKDIRFAL PGDFSRRAFQ NGKFDLTQLE 190 200 210 220 230 240 GIKDLIDSET ESQRRSALSS FNGDNKILFE NWRETIIENM AQLTAIIDFA DDNSQEIQNT 250 260 270 280 290 300 DEIFHNVEKN IICLRDQIVT FMQKVEKSTI LQNGIKLVLL GAPNVGKSSL VNSLTNDDIS 310 320 330 340 350 360 IVSDIPGTTR DSIDAMINVN GYKVIICDTA GIREKSSDKI EMLGIDRAKK KSVQSDLCLF 370 380 390 400 410 420 IVDPTDLSKL LPEDILAHLS SKTFGNKRII IVVNKSDLVS DDEMTKVLNK LQTRLGSKYP 430 440 450 460 470 480 ILSVSCKTKE GIESLISTLT SNFESLSQSS ADASPVIVSK RVSEILKNDV LYGLEEFFKS 490 500 510 520 KDFHNDIVLA TENLRYASDG IAKITGQAIG IEEILDSVFS KFCIGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MSS1, a nuclear-encoded mitochondrial GTPase involved in the expression of COX1 subunit of cytochrome c oxidase." Decoster E., Vassal A., Faye G. J. Mol. Biol. 232:79-88(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"MSS1, a mitochondrial GTP-binding protein involved in mitochondrial tRNA modification." Li X., Wang X., Guan M.-X. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"MTO1 codes for a mitochondrial protein required for respiration in paromomycin-resistant mutants of Saccharomyces cerevisiae." Colby G., Wu M., Tzagoloff A. J. Biol. Chem. 273:27945-27952(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MTO1.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Mitochondria-specific RNA-modifying enzymes responsible for the biosynthesis of the wobble base in mitochondrial tRNAs. Implications for the molecular pathogenesis of human mitochondrial diseases." Umeda N., Suzuki T., Yukawa M., Ohya Y., Shindo H., Watanabe K., Suzuki T. J. Biol. Chem. 280:1613-1624(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X69481 Genomic DNA. Translation: CAA49238.1. DQ834923 Genomic DNA. Translation: ABG77646.1. Z49211 Genomic DNA. Translation: CAA89126.1. BK006946 Genomic DNA. Translation: DAA09921.1.
PIR	S54025.
RefSeq	NP_013736.1. NM_001182519.1.
3D structure databases
ProteinModelPortal	P32559.
SMR	P32559. Positions 38-526.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5882N.
IntAct	P32559. 2 interactions.
MINT	MINT-2788166.
STRING	4932.YMR023C.
Proteomic databases
PaxDb	P32559.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR023C; YMR023C; YMR023C.
GeneID	855037.
KEGG	sce:YMR023C.
Organism-specific databases
CYGD	YMR023c.
SGD	S000004625. MSS1.
Phylogenomic databases
eggNOG	COG0486.
GeneTree	ENSGT00390000016851.
HOGENOM	HOG000200714.
KO	K03650.
OMA	IRISGTH.
OrthoDB	EOG4B2X62.
Gene expression databases
Genevestigator	P32559.
GermOnline	YMR023C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.1360.120. 1 hit.
InterPro	IPR018948. GTP-bd_TrmE_N. IPR006073. GTP_binding_domain. IPR004520. GTPase_MnmE. IPR025867. GTPase_MnmE_C_dom. IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR027266. TrmE/GcvT_dom1. [Graphical view]
Pfam	PF12631. GTPase_Cys_C. 1 hit. PF01926. MMR_HSR1. 1 hit. PF10396. TrmE_N. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
TIGRFAMs	TIGR00450. mnmE_trmE_thdF. 1 hit. TIGR00231. small_GTP. 1 hit.
ProtoNet	Search...
Other
NextBio	978251.

Entry information

Entry name

MSS1_YEAST

Accession

Primary (citable) accession number: P32559
Secondary accession number(s): D6VZJ7, Q0PHA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program