Pol polyprotein - Equine infectious anemia virus (isolate CL22) (EIAV)

Sequence

>sp|P32542|POL_EIAVC Pol polyprotein OS=Equine infectious anemia virus (isolate CL22) GN=pol PE=1 SV=1 TAWTFLKAMQKCSKKREARGSREAPETNFPDTTEESAQQICCTRDSSDSKSVPRSERNKK GIQCQGEGSSRGSQPGQFVGVTYNLEKRPTTIVLINDTPLNVLLDTGADTSVLTTAHYNR LKYRGRKYQGTGIIGVGGNVETFSTPVTIKKKGRHIKTRMLVADIPVTILGRDILQDLGA KLVLAQLSKEIKFRKIELKEGTMGPKIPQWPLTKEKLEGAKEIVQRLLSEGKISEASDNN PYNSPIFVIKKRSGKWRLLQDLRELNKTVQVGTEISRGLPHPGGLIKCKHMTVLDIGDAY FTIPLDPEFRPYTAFTIPSINHQEPDKRYVWNCLPQGFVLSPYIYQKTLQEILQPFRERY PEVQLYQYMDDLFVGSNGSKKQHKELIIELRAILLEKGFETPDDKLQEVPPYSWLGYQLC PENWKVQKMQLDMVKNPTLNDVQKLMGNITWMSSGVPGLTVKHIAATTKGCLELNQKVIW TEEAQKELEENNEKIKNAQGLQYYNPEEEMLCEVEITKNYEATYVIKQSQGILWAGKKIM KANKGWSTVKNLMLLLQHVATESITRVGKCPTFKVPFTKEQVMWEMQKGWYYSWLPEIVY THQVVHDDWRMKLVEEPTSGITIYTDGGKQNGEGIAAYVTSNGRTKQKRLGPVTHQVAER MAIQMALEDTRDKQVNIVTDSYYCWKNITEGLGLEGPQSPWWPIIQNIREKEIVYFAWVP GHKGICGNQLADEAAKIKEEIMLAYQGTQIKEKRDEDAGFDLCVPYDIMIPVSDTKIIPT DVKIQVPPNSFGWVTGKSSMAKQGLLINGGIIDEGYTGEIQVICTNIGKSNIKLIEGQKF AQLIILQHHSNSRQPWDENKISQRGDKGFGSTGVFWVENIQEAQDEHENWHTSPKILARN YKIPLTVAKQITQECPHCTKQGSGPAGCVMRSPNHWQADCTHLDNKIILTFVESNSGYIH ATLLSKENALCTSLAILEWARLFSPKSLHTDNGTNFVAEPVVNLLKFLKIAHTTGIPYHP ESQGIVERANRTLKEKIQSHRDNTQTLEAALQLALITCNKGRESMGGQTPWEVFITNQAQ VIHEKLLLQQAQSSKKFCFYKIPGEHDWKGPTRVLWKGDGAVVVNDEGKGIIAVPLTRTK LLIKPN

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Reviewed, UniProtKB/Swiss-Prot P32542 (POL_EIAVC)

Last modified June 16, 2009. Version 88. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Pol polyprotein
Cleaved into the following 3 chains:
    1- Recommended name:
            Protease
        Alternative name(s):
            Retropepsin
            EC=3.4.23.-
    2- Recommended name:
            Reverse transcriptase/ribonuclease H
                Short name=RT
              EC=2.7.7.49
              EC=3.1.26.4
    3- Recommended name:
            Integrase
                Short name=IN

Gene names

Name:

pol

Organism

Equine infectious anemia virus (isolate CL22) (EIAV)

Taxonomic identifier

31675 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Lentivirus › Equine lentivirus group

Virus host

Equus asinus (Donkey) [TaxID: 9793]
Equus caballus (Horse) [TaxID: 9796]

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Protein attributes

Sequence length	1146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Post-translational modification	Specific enzymatic cleavages in vivo yield mature proteins.
Sequence similarities	Belongs to the retroviral Pol polyprotein family. Contains 1 integrase catalytic domain. Contains 1 integrase-type DNA-binding domain. Contains 1 integrase-type zinc finger. Contains 1 peptidase A2 domain. Contains 1 reverse transcriptase domain. Contains 1 RNase H domain.

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Ontologies

Keywords
Biological process	DNA integration DNA recombination
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Aspartyl protease Endonuclease Hydrolase Nuclease Nucleotidyltransferase Protease RNA-directed DNA polymerase Transferase
PTM	Cleavage on pair of basic residues
Technical term	3D-structure Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA integration Inferred from electronic annotation. Source: InterPro DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW RNA-dependent DNA replication Inferred from electronic annotation. Source: InterPro dUTP metabolic process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro transposition Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW integrase activity Inferred from electronic annotation. Source: InterPro ribonuclease H activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 195

195

Protease

PRO_0000038832

Chain

196 – 914

719

Reverse transcriptase/ribonuclease H

PRO_0000038833

Chain

915 – 1146

232

Integrase

PRO_0000038834

Regions

Domain

100 – 174

Peptidase A2

Domain

230 – 419

190

Reverse transcriptase

Domain

617 – 740

124

RNase H

Domain

921 – 1078

158

Integrase catalytic

Zinc finger

878 – 919

Integrase-type

DNA binding

1096 – 1144

Integrase-type

Sites

Active site

105

By similarity

Secondary structure

1146

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P32542-1 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: C14058EAF93F3F5D
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FASTA

1,146

129,509

        10         20         30         40         50         60 
TAWTFLKAMQ KCSKKREARG SREAPETNFP DTTEESAQQI CCTRDSSDSK SVPRSERNKK 

        70         80         90        100        110        120 
GIQCQGEGSS RGSQPGQFVG VTYNLEKRPT TIVLINDTPL NVLLDTGADT SVLTTAHYNR 

       130        140        150        160        170        180 
LKYRGRKYQG TGIIGVGGNV ETFSTPVTIK KKGRHIKTRM LVADIPVTIL GRDILQDLGA 

       190        200        210        220        230        240 
KLVLAQLSKE IKFRKIELKE GTMGPKIPQW PLTKEKLEGA KEIVQRLLSE GKISEASDNN 

       250        260        270        280        290        300 
PYNSPIFVIK KRSGKWRLLQ DLRELNKTVQ VGTEISRGLP HPGGLIKCKH MTVLDIGDAY 

       310        320        330        340        350        360 
FTIPLDPEFR PYTAFTIPSI NHQEPDKRYV WNCLPQGFVL SPYIYQKTLQ EILQPFRERY 

       370        380        390        400        410        420 
PEVQLYQYMD DLFVGSNGSK KQHKELIIEL RAILLEKGFE TPDDKLQEVP PYSWLGYQLC 

       430        440        450        460        470        480 
PENWKVQKMQ LDMVKNPTLN DVQKLMGNIT WMSSGVPGLT VKHIAATTKG CLELNQKVIW 

       490        500        510        520        530        540 
TEEAQKELEE NNEKIKNAQG LQYYNPEEEM LCEVEITKNY EATYVIKQSQ GILWAGKKIM 

       550        560        570        580        590        600 
KANKGWSTVK NLMLLLQHVA TESITRVGKC PTFKVPFTKE QVMWEMQKGW YYSWLPEIVY 

       610        620        630        640        650        660 
THQVVHDDWR MKLVEEPTSG ITIYTDGGKQ NGEGIAAYVT SNGRTKQKRL GPVTHQVAER 

       670        680        690        700        710        720 
MAIQMALEDT RDKQVNIVTD SYYCWKNITE GLGLEGPQSP WWPIIQNIRE KEIVYFAWVP 

       730        740        750        760        770        780 
GHKGICGNQL ADEAAKIKEE IMLAYQGTQI KEKRDEDAGF DLCVPYDIMI PVSDTKIIPT 

       790        800        810        820        830        840 
DVKIQVPPNS FGWVTGKSSM AKQGLLINGG IIDEGYTGEI QVICTNIGKS NIKLIEGQKF 

       850        860        870        880        890        900 
AQLIILQHHS NSRQPWDENK ISQRGDKGFG STGVFWVENI QEAQDEHENW HTSPKILARN 

       910        920        930        940        950        960 
YKIPLTVAKQ ITQECPHCTK QGSGPAGCVM RSPNHWQADC THLDNKIILT FVESNSGYIH 

       970        980        990       1000       1010       1020 
ATLLSKENAL CTSLAILEWA RLFSPKSLHT DNGTNFVAEP VVNLLKFLKI AHTTGIPYHP 

      1030       1040       1050       1060       1070       1080 
ESQGIVERAN RTLKEKIQSH RDNTQTLEAA LQLALITCNK GRESMGGQTP WEVFITNQAQ 

      1090       1100       1110       1120       1130       1140 
VIHEKLLLQQ AQSSKKFCFY KIPGEHDWKG PTRVLWKGDG AVVVNDEGKG IIAVPLTRTK 


LLIKPN

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References

[1]	"The surface envelope protein gene region of equine infectious anemia virus is not an important determinant of tropism in vitro." Perry S.T., Flaherty M.T., Kelley M.J., Clabough D.L., Tronick S.R., Coggins L., Whetter L., Lengel C.R., Fuller F. J. Virol. 66:4085-4097(1992) [PubMed: 1318398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Molecular model of equine infectious anemia virus proteinase and kinetic measurements for peptide substrates with single amino acid substitutions." Weber I.T., Tozser J., Wu J., Friedman D., Oroszlan S. Biochemistry 32:3354-3362(1993) [PubMed: 8384880] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 81-184.
[3]	"Structure of equine infectious anemia virus proteinase complexed with an inhibitor." Gustchina A., Kervinen J., Powell D.J., Zdanov A., Kay J., Wlodawer A. Protein Sci. 5:1453-1465(1996) [PubMed: 8844837] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 81-184.

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Cross-references

Sequence databases

M87581 Genomic RNA. Translation: AAA43004.1.

PIR

GNLJ22. B41991.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EQI	model	-	A/B	81-184	[»]
1FMB	X-ray	1.80	A	81-184	[»]
2FMB	X-ray	1.80	A	81-184	[»]

SMR

P32542. Positions 81-184, 742-861.

ModBase

Search...

Protein family/group databases

MEROPS

A02.004.

Family and domain databases

InterPro

IPR008180. DeoxyUTPase.
IPR000477. DNA_pol_RVTase.
IPR001037. Integrase_C_retrovir.
IPR001584. Integrase_cat-core.
IPR017856. Integrase_Zn-bd_dom-like_N.
IPR003308. Integrase_Zn-bd_dom_N.
IPR018061. Pept_A2A_retrovirus_sg.
IPR001995. Peptidase_A2_cat.
IPR001969. Peptidase_aspartic_AS.
IPR009007. Peptidase_aspartic_catalytic.
IPR002156. RNase_H.
IPR010659. RVT_connect.
IPR010661. RVT_thumb.
[Graphical view]

Gene3D

G3DSA:2.30.30.10. Integrase_C. 1 hit.
G3DSA:1.10.10.200. Intgrase_N_Zn_bd. 1 hit.
G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.

Pfam

PF00692. dUTPase. 1 hit.
PF00552. Integrase. 1 hit.
PF02022. Integrase_Zn. 1 hit.
PF00075. RnaseH. 1 hit.
PF00665. rve. 1 hit.
PF00077. RVP. 1 hit.
PF00078. RVT_1. 1 hit.
PF06815. RVT_connect. 1 hit.
PF06817. RVT_thumb. 1 hit.
[Graphical view]

ProDom

PD004900. dCTP_deaminase. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS50175. ASP_PROT_RETROV. 1 hit.
PS00141. ASP_PROTEASE. 1 hit.
PS50994. INTEGRASE. 1 hit.
PS51027. INTEGRASE_DBD. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
PS50876. ZF_INTEGRASE. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

POL_EIAVC

Accession

Primary (citable) accession number: P32542

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families