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Protein

Pol polyprotein

Gene

pol

Organism
Equine infectious anemia virus (isolate CL22) (EIAV)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.

Catalytic activityi

Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei105PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri878 – 919Integrase-typePROSITE-ProRule annotationAdd BLAST42
DNA bindingi1096 – 1144Integrase-typePROSITE-ProRule annotationAdd BLAST49

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAspartyl protease, DNA-binding, Endonuclease, Hydrolase, Multifunctional enzyme, Nuclease, Nucleotidyltransferase, Protease, RNA-directed DNA polymerase, Transferase
Biological processDNA integration, DNA recombination, Viral genome integration, Virus entry into host cell
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.23.B3 2115

Protein family/group databases

MEROPSiA02.004

Names & Taxonomyi

Protein namesi
Recommended name:
Pol polyprotein
Cleaved into the following 3 chains:
Alternative name(s):
Retropepsin (EC:3.4.23.-)
Alternative name(s):
Exoribonuclease H (EC:3.1.13.2)
Integrase (EC:2.7.7.-By similarity, EC:3.1.-.-By similarity)
Short name:
IN
Gene namesi
Name:pol
OrganismiEquine infectious anemia virus (isolate CL22) (EIAV)
Taxonomic identifieri31675 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeLentivirusBovine lentivirus group
Virus hostiEquus asinus (Donkey) (Equus africanus asinus) [TaxID: 9793]
Equus caballus (Horse) [TaxID: 9796]

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000388321 – 195ProteaseAdd BLAST195
ChainiPRO_0000038833196 – 914Reverse transcriptase/ribonuclease HAdd BLAST719
ChainiPRO_0000038834915 – 1146IntegraseAdd BLAST232

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins.

Keywords - PTMi

Cleavage on pair of basic residues

Proteomic databases

PRIDEiP32542

Structurei

Secondary structure

11146
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi85 – 87Combined sources3
Beta strandi90 – 95Combined sources6
Beta strandi98 – 104Combined sources7
Beta strandi112 – 114Combined sources3
Helixi115 – 120Combined sources6
Beta strandi133 – 135Combined sources3
Beta strandi138 – 140Combined sources3
Beta strandi143 – 151Combined sources9
Beta strandi154 – 164Combined sources11
Helixi172 – 177Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EQImodel-A/B81-184[»]
1FMBX-ray1.80A81-184[»]
2FMBX-ray1.80A81-184[»]
ProteinModelPortaliP32542
SMRiP32542
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32542

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini100 – 174Peptidase A2PROSITE-ProRule annotationAdd BLAST75
Domaini230 – 419Reverse transcriptasePROSITE-ProRule annotationAdd BLAST190
Domaini617 – 740RNase HPROSITE-ProRule annotationAdd BLAST124
Domaini921 – 1078Integrase catalyticPROSITE-ProRule annotationAdd BLAST158

Sequence similaritiesi

Belongs to the retroviral Pol polyprotein family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri878 – 919Integrase-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Zinc-finger

Family and domain databases

CDDicd07557 trimeric_dUTPase, 1 hit
Gene3Di1.10.10.200, 1 hit
2.30.30.10, 1 hit
2.40.70.10, 1 hit
2.70.40.10, 1 hit
3.30.420.10, 2 hits
InterProiView protein in InterPro
IPR001969 Aspartic_peptidase_AS
IPR029054 dUTPase-like
IPR036157 dUTPase-like_sf
IPR033704 dUTPase_trimeric
IPR017856 Integrase-like_N
IPR036862 Integrase_C_dom_sf_retrovir
IPR001037 Integrase_C_retrovir
IPR001584 Integrase_cat-core
IPR003308 Integrase_Zn-bd_dom_N
IPR001995 Peptidase_A2_cat
IPR021109 Peptidase_aspartic_dom_sf
IPR018061 Retropepsins
IPR012337 RNaseH-like_sf
IPR002156 RNaseH_domain
IPR036397 RNaseH_sf
IPR000477 RT_dom
IPR010659 RVT_connect
IPR010661 RVT_thumb
PfamiView protein in Pfam
PF00692 dUTPase, 1 hit
PF00552 IN_DBD_C, 1 hit
PF02022 Integrase_Zn, 1 hit
PF00075 RNase_H, 1 hit
PF00665 rve, 1 hit
PF00077 RVP, 1 hit
PF00078 RVT_1, 1 hit
PF06815 RVT_connect, 1 hit
PF06817 RVT_thumb, 1 hit
SUPFAMiSSF46919 SSF46919, 1 hit
SSF50122 SSF50122, 1 hit
SSF50630 SSF50630, 1 hit
SSF51283 SSF51283, 1 hit
SSF53098 SSF53098, 2 hits
PROSITEiView protein in PROSITE
PS50175 ASP_PROT_RETROV, 1 hit
PS00141 ASP_PROTEASE, 1 hit
PS50994 INTEGRASE, 1 hit
PS51027 INTEGRASE_DBD, 1 hit
PS50879 RNASE_H, 1 hit
PS50878 RT_POL, 1 hit
PS50876 ZF_INTEGRASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32542-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
TAWTFLKAMQ KCSKKREARG SREAPETNFP DTTEESAQQI CCTRDSSDSK
60 70 80 90 100
SVPRSERNKK GIQCQGEGSS RGSQPGQFVG VTYNLEKRPT TIVLINDTPL
110 120 130 140 150
NVLLDTGADT SVLTTAHYNR LKYRGRKYQG TGIIGVGGNV ETFSTPVTIK
160 170 180 190 200
KKGRHIKTRM LVADIPVTIL GRDILQDLGA KLVLAQLSKE IKFRKIELKE
210 220 230 240 250
GTMGPKIPQW PLTKEKLEGA KEIVQRLLSE GKISEASDNN PYNSPIFVIK
260 270 280 290 300
KRSGKWRLLQ DLRELNKTVQ VGTEISRGLP HPGGLIKCKH MTVLDIGDAY
310 320 330 340 350
FTIPLDPEFR PYTAFTIPSI NHQEPDKRYV WNCLPQGFVL SPYIYQKTLQ
360 370 380 390 400
EILQPFRERY PEVQLYQYMD DLFVGSNGSK KQHKELIIEL RAILLEKGFE
410 420 430 440 450
TPDDKLQEVP PYSWLGYQLC PENWKVQKMQ LDMVKNPTLN DVQKLMGNIT
460 470 480 490 500
WMSSGVPGLT VKHIAATTKG CLELNQKVIW TEEAQKELEE NNEKIKNAQG
510 520 530 540 550
LQYYNPEEEM LCEVEITKNY EATYVIKQSQ GILWAGKKIM KANKGWSTVK
560 570 580 590 600
NLMLLLQHVA TESITRVGKC PTFKVPFTKE QVMWEMQKGW YYSWLPEIVY
610 620 630 640 650
THQVVHDDWR MKLVEEPTSG ITIYTDGGKQ NGEGIAAYVT SNGRTKQKRL
660 670 680 690 700
GPVTHQVAER MAIQMALEDT RDKQVNIVTD SYYCWKNITE GLGLEGPQSP
710 720 730 740 750
WWPIIQNIRE KEIVYFAWVP GHKGICGNQL ADEAAKIKEE IMLAYQGTQI
760 770 780 790 800
KEKRDEDAGF DLCVPYDIMI PVSDTKIIPT DVKIQVPPNS FGWVTGKSSM
810 820 830 840 850
AKQGLLINGG IIDEGYTGEI QVICTNIGKS NIKLIEGQKF AQLIILQHHS
860 870 880 890 900
NSRQPWDENK ISQRGDKGFG STGVFWVENI QEAQDEHENW HTSPKILARN
910 920 930 940 950
YKIPLTVAKQ ITQECPHCTK QGSGPAGCVM RSPNHWQADC THLDNKIILT
960 970 980 990 1000
FVESNSGYIH ATLLSKENAL CTSLAILEWA RLFSPKSLHT DNGTNFVAEP
1010 1020 1030 1040 1050
VVNLLKFLKI AHTTGIPYHP ESQGIVERAN RTLKEKIQSH RDNTQTLEAA
1060 1070 1080 1090 1100
LQLALITCNK GRESMGGQTP WEVFITNQAQ VIHEKLLLQQ AQSSKKFCFY
1110 1120 1130 1140
KIPGEHDWKG PTRVLWKGDG AVVVNDEGKG IIAVPLTRTK LLIKPN
Length:1,146
Mass (Da):129,509
Last modified:October 1, 1993 - v1
Checksum:iC14058EAF93F3F5D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87581 Genomic RNA Translation: AAA43004.1
PIRiB41991 GNLJ22

Similar proteinsi

Entry informationi

Entry nameiPOL_EIAVC
AccessioniPrimary (citable) accession number: P32542
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: March 28, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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