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Protein

Zuotin

Gene

ZUO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ribosome-associated complex (RAC), a heterodimeric chaperone complex involved in regulation of accurate translation termination and in folding or maintaining nascent polypeptides in a folding-competent state. RAC stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones SSB1/SSB2 that bind to the nascent polypeptide chain. ZUO1 can act as a J-protein for SSB1/SSB2 only when associated with SSZ1.2 Publications

Miscellaneous

Present with 86400 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • ribosome binding Source: SGD
  • unfolded protein binding Source: SGD

GO - Biological processi

  • 'de novo' cotranslational protein folding Source: SGD
  • cellular response to cycloheximide Source: CAFA
  • negative regulation of cell death Source: CAFA
  • positive regulation of transcription from RNA polymerase II promoter in response to stress Source: CAFA
  • protein folding Source: SGD
  • regulation of translational fidelity Source: SGD
  • ribosomal subunit export from nucleus Source: SGD
  • rRNA processing Source: SGD
  • translational frameshifting Source: SGD

Keywordsi

Molecular functionChaperone, DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30947-MONOMER
ReactomeiR-SCE-3371453 Regulation of HSF1-mediated heat shock response

Protein family/group databases

MoonProtiP32527

Names & Taxonomyi

Protein namesi
Recommended name:
Zuotin
Alternative name(s):
DnaJ-related protein ZUO1
Short name:
J protein ZUO1
Heat shock protein 40 homolog ZUO1
Ribosome-associated complex subunit ZUO1
Gene namesi
Name:ZUO1
Ordered Locus Names:YGR285C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR285C
SGDiS000003517 ZUO1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi128H → Q: Loss of function, but still forms a heterodimer with SSZ1 and associates with ribosomes. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000711221 – 433ZuotinAdd BLAST433

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei50PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32527
PaxDbiP32527
PRIDEiP32527

PTM databases

iPTMnetiP32527

Interactioni

Subunit structurei

RAC is a heterodimer of the Hsp70/DnaK-type chaperone SSZ1 and the Hsp40/DnaJ-type chaperone ZUO1. RAC associates with ribosomes via ZUO1.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • unfolded protein binding Source: SGD

Protein-protein interaction databases

BioGridi33535, 119 interactors
DIPiDIP-4720N
IntActiP32527, 139 interactors
MINTiP32527
STRINGi4932.YGR285C

Structurei

Secondary structure

1433
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi184 – 196Combined sources13
Beta strandi199 – 203Combined sources5
Helixi214 – 225Combined sources12
Helixi234 – 236Combined sources3
Helixi249 – 282Combined sources34
Helixi284 – 300Combined sources17
Helixi348 – 364Combined sources17
Helixi371 – 373Combined sources3
Helixi374 – 387Combined sources14
Helixi390 – 402Combined sources13
Helixi404 – 407Combined sources4
Helixi408 – 420Combined sources13
Helixi426 – 429Combined sources4
Turni430 – 432Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LWXNMR-A348-433[»]
5DJEX-ray1.85A/B166-303[»]
ProteinModelPortaliP32527
SMRiP32527
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini98 – 170JPROSITE-ProRule annotationAdd BLAST73

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi306 – 357Ala/Lys-richAdd BLAST52

Phylogenomic databases

GeneTreeiENSGT00530000063419
HOGENOMiHOG000171054
InParanoidiP32527
KOiK09522
OMAiRTFSEDD
OrthoDBiEOG092C36RH

Family and domain databases

CDDicd06257 DnaJ, 1 hit
Gene3Di1.10.287.110, 1 hit
InterProiView protein in InterPro
IPR001623 DnaJ_domain
IPR018253 DnaJ_domain_CS
IPR036869 J_dom_sf
IPR032003 RAC_head
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF16717 RAC_head, 1 hit
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
PROSITEiView protein in PROSITE
PS00636 DNAJ_1, 1 hit
PS50076 DNAJ_2, 1 hit

Sequencei

Sequence statusi: Complete.

P32527-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSLPTLTSD ITVEVNSSAT KTPFVRRPVE PVGKFFLQHA QRTLRNHTWS
60 70 80 90 100
EFERIEAEKN VKTVDESNVD PDELLFDTEL ADEDLLTHDA RDWKTADLYA
110 120 130 140 150
AMGLSKLRFR ATESQIIKAH RKQVVKYHPD KQSAAGGSLD QDGFFKIIQK
160 170 180 190 200
AFETLTDSNK RAQYDSCDFV ADVPPPKKGT DYDFYEAWGP VFEAEARFSK
210 220 230 240 250
KTPIPSLGNK DSSKKEVEQF YAFWHRFDSW RTFEFLDEDV PDDSSNRDHK
260 270 280 290 300
RYIERKNKAA RDKKKTADNA RLVKLVERAV SEDPRIKMFK EEEKKEKERR
310 320 330 340 350
KWEREAGARA EAEAKAKAEA EAKAKAESEA KANASAKADK KKAKEAAKAA
360 370 380 390 400
KKKNKRAIRN SAKEADYFGD ADKATTIDEQ VGLIVDSLND EELVSTADKI
410 420 430
KANAAGAKEV LKESAKTIVD SGKLPSSLLS YFV
Length:433
Mass (Da):49,020
Last modified:October 1, 1993 - v1
Checksum:i0AA76BC11D3C7DAB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63612 Genomic DNA Translation: CAA45156.1
Z73070 Genomic DNA Translation: CAA97317.1
AY692823 Genomic DNA Translation: AAT92842.1
BK006941 Genomic DNA Translation: DAA08373.1
PIRiS25194
RefSeqiNP_011801.1, NM_001181414.1

Genome annotation databases

EnsemblFungiiYGR285C; YGR285C; YGR285C
GeneIDi853202
KEGGisce:YGR285C

Similar proteinsi

Entry informationi

Entry nameiZUO1_YEAST
AccessioniPrimary (citable) accession number: P32527
Secondary accession number(s): D6VV62
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: April 25, 2018
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health