P32524 (PRP21_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre-mRNA-splicing factor PRP21

Gene names

Name:	PRP21
Synonyms:	SPP91
Ordered Locus Names:	YJL203W
ORF Names:	J0322

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	mRNA splicing factors, PRP9, PRP11, and PRP21, are necessary for binding of the U2 snRNP to the pre-mRNA in an early step of spliceosome assembly.
Subcellular location	Nucleus.
Miscellaneous	Present with 2490 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Contains 2 SURP motif repeats.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Domain	Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nuclear mRNA splicing, via spliceosome Inferred from direct assay. Source: SGD
Cellular component	U2 snRNP Traceable author statement. Source: SGD U2-type prespliceosome Inferred from direct assay. Source: SGD
Molecular function	RNA binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 280	280	Pre-mRNA-splicing factor PRP21		PRO_0000174322
Regions
	Repeat	11 – 49	39	SURP motif 1
	Repeat	95 – 135	41	SURP motif 2
Amino acid modifications
	Modified residue	61	1	Phosphoserine Ref.8
	Modified residue	147	1	Phosphoserine Ref.8
	Modified residue	250	1	Phosphoserine Ref.8
Experimental info
	Mutagenesis	168	1	T → A in SPP91-1; corrects the PRP9-1 growth defect through partial restoration of splicing and by a complete reversion of the pre-mRNA escape phenotype.
	Sequence conflict	205	1	K → N in AAS56405. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P32524 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 2E9DBA6F06759B3F	FASTA	280	33,052
	10 20 30 40 50 60 MEPEDTQLKE DIKTTVNYIK QHGVEFENKL LEDERFSFIK KDDPLHEYYT KLMNEPTDTV 70 80 90 100 110 120 SGEDNDRKSE REIARPPDFL FSQYDTGISR RDMEVIKLTA RYYAKDKSIV EQMISKDGEA 130 140 150 160 170 180 RLNFMNSSHP LHKTFTDFVA QYKRVYSFTG QEIKKSKRTI LDNCFERTQY WEFEKDKDRE 190 200 210 220 230 240 HDKLVELCKI QFAAIPWDKF TQVAKFSIPE DTEIFEGSLD LEQMRLRRVQ TGIKLFDSIK 250 260 270 280 PTNEEEKIVS DQGKQKGGDS KGKKRKIRAV GETRLKKSKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel gene, spp91-1, suppresses the splicing defect and the pre-mRNA nuclear export in the prp9-1 mutant." Chapon C., Legrain P. EMBO J. 11:3279-3288(1992) [PubMed: 1505518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTANT SPP91-1. Strain: CY183.
[2]	"The Saccharomyces cerevisiae PRP21 gene product is an integral component of the prespliceosome." Arenas J.E., Abelson J.N. Proc. Natl. Acad. Sci. U.S.A. 90:6771-6775(1993) [PubMed: 8341697] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The sequence of a 36 kb segment on the left arm of yeast chromosome X identifies 24 open reading frames including NUC1, PRP21 (SPP91), CDC6, CRY2, the gene for S24, a homologue to the aconitase gene ACO1 and two homologues to chromosome III genes." Purnelle B., Coster F., Goffeau A. Yeast 10:1235-1249(1994) [PubMed: 7754713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-61; SER-147 AND SER-250, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X67564 Genomic DNA. Translation: CAA47860.1. L07744 Genomic DNA. Translation: AAB09601.1. X77688 Genomic DNA. Translation: CAA54754.1. Z49478 Genomic DNA. Translation: CAA89497.1. AY558079 Genomic DNA. Translation: AAS56405.1. BK006943 Genomic DNA. Translation: DAA08607.1.
PIR	S23553.
RefSeq	NP_012332.1. NM_001181636.1.
3D structure databases
ProteinModelPortal	P32524.
SMR	P32524. Positions 9-57, 75-144.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-33054N. DIP-689N.
IntAct	P32524. 24 interactions.
MINT	MINT-383990.
STRING	P32524.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL203W; YJL203W; YJL203W.
GeneID	853227.
KEGG	sce:YJL203W.
NMPDR	fig|4932.3.peg.3295.
Organism-specific databases
CYGD	YJL203w.
SGD	S000003739. PRP21.
Phylogenomic databases
eggNOG	fuNOG10589.
GeneTree	EFGT00050000006626.
OMA	RRKENDP.
OrthoDB	EOG40S3R5.
Gene expression databases
ArrayExpress	P32524.
Genevestigator	P32524.
GermOnline	YJL203W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR022030. PRP21-like. IPR000061. Surp. [Graphical view]
Pfam	PF12230. PRP21_like_P. 1 hit. PF01805. Surp. 2 hits. [Graphical view]
SMART	SM00648. SWAP. 2 hits. [Graphical view]
PROSITE	PS50128. SURP. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	973437.

Entry information

Entry name

PRP21_YEAST

Accession

Primary (citable) accession number: P32524
Secondary accession number(s): D6VVZ1, Q6Q5G7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	December 14, 2011
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program