Pre-mRNA-splicing factor 19 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P32523 (PRP19_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 108. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Pre-mRNA-splicing factor 19

Gene names

Name:	PRP19
Synonyms:	PSO4
Ordered Locus Names:	YLL036C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in pre-mRNA splicing. Acts a central component of the NTC complex (or PRP19-associated complex) that associates to the spliceosome to mediate conformational rearrangement or to stabilize the structure of the spliceosome after U4 snRNA dissociation, which leads to spliceosome maturation. Involved in DNA repair.
Subunit structure	Forms homotetramers. Belongs to the NTC complex (or PRP19-associated complex), composed of at least CEF1, CLF1, ISY1, NTC20, SNT309, SYF1, SYF2, and PRP19. Ref.8
Subcellular location	Nucleus.
Miscellaneous	Present with 11700 molecules/cell in log phase SD medium. Ref.7 The tetramer is an elongated particle consisting of four globular WD40 domains held together by a central stalk.
Sequence similarities	Belongs to the WD repeat PRP19 family. Contains 1 U-box domain. Contains 3 WD repeats.

Ontologies

Keywords
Biological process	DNA damage DNA repair mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW generation of catalytic spliceosome for first transesterification step Inferred from mutant phenotype. Source: SGD
Cellular component	Prp19 complex Inferred from direct assay Ref.6. Source: SGD U2-type catalytic step 1 spliceosome Inferred from direct assay. Source: SGD mitochondrion Inferred from direct assay. Source: SGD ubiquitin ligase complex Inferred from electronic annotation. Source: InterPro
Molecular function	protein binding Inferred from physical interaction. Source: IntAct ubiquitin-protein ligase activity Inferred from direct assay Ref.11. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
CDC40	P40968	7	EBI-493,EBI-13834
CEF1	Q03654	9	EBI-493,EBI-476
CLF1	Q12309	10	EBI-493,EBI-484
CWC2	Q12046	9	EBI-493,EBI-553
ISY1	P21374	6	EBI-493,EBI-9382
NTC20	P38302	5	EBI-493,EBI-20921
SNT309	Q06091	8	EBI-493,EBI-818
SYF1	Q04048	6	EBI-493,EBI-540
SYF2	P53277	4	EBI-493,EBI-23308
URN1	Q06525	7	EBI-493,EBI-35138
YLR132C	Q12208	3	EBI-493,EBI-31589

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 503

503

Pre-mRNA-splicing factor 19

PRO_0000058587

Regions

Domain

1 – 64

U-box

Repeat

328 – 367

WD 1

Repeat

372 – 410

WD 2

Repeat

418 – 458

WD 3

Region

57 – 144

Required for self-association

Region

76 – 134

Interaction with CEF1

Amino acid modifications

Modified residue

140

Phosphoserine Ref.10

Modified residue

141

Phosphoserine; by ATM or ATR Ref.9 Ref.10

Experimental info

Sequence conflict

239

Missing in AAA34912. Ref.1

Secondary structure

503

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P32523 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: A44D8C7DD6F64185
Show »

FASTA

503

56,570

        10         20         30         40         50         60 
MLCAISGKVP RRPVLSPKSR TIFEKSLLEQ YVKDTGNDPI TNEPLSIEEI VEIVPSAQQA 

        70         80         90        100        110        120 
SLTESTNSAT LKANYSIPNL LTSLQNEWDA IMLENFKLRS TLDSLTKKLS TVMYERDAAK 

       130        140        150        160        170        180 
LVAAQLLMEK NEDSKDLPKS SQQAVAITRE EFLQGLLQSS RDFVARGKLK APKWPILKNL 

       190        200        210        220        230        240 
ELLQAQNYSR NIKTFPYKEL NKSMYYDKWV CMCRCEDGAL HFTQLKDSKT ITTITTPNPR 

       250        260        270        280        290        300 
TGGEHPAIIS RGPCNRLLLL YPGNQITILD SKTNKVLREI EVDSANEIIY MYGHNEVNTE 

       310        320        330        340        350        360 
YFIWADNRGT IGFQSYEDDS QYIVHSAKSD VEYSSGVLHK DSLLLALYSP DGILDVYNLS 

       370        380        390        400        410        420 
SPDQASSRFP VDEEAKIKEV KFADNGYWMV VECDQTVVCF DLRKDVGTLA YPTYTIPEFK 

       430        440        450        460        470        480 
TGTVTYDIDD SGKNMIAYSN ESNSLTIYKF DKKTKNWTKD EESALCLQSD TADFTDMDVV 

       490        500 
CGDGGIAAIL KTNDSFNIVA LTP

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PRP19: a novel spliceosomal component." Cheng S.C., Tarn W.Y., Tsao T.Y., Abelson J. Mol. Cell. Biol. 13:1876-1882(1993) [PubMed: 8441419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Allelism of PSO4 and PRP19 links pre-mRNA processing with recombination and error-prone DNA repair in Saccharomyces cerevisiae." Grey M., Duesterhoeft A., Henriques J.A.P., Brendel M. Nucleic Acids Res. 24:4009-4014(1996) [PubMed: 8918805] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"The yeast PRP19 protein is not tightly associated with small nuclear RNAs, but appears to associate with the spliceosome after binding of U2 to the pre-mRNA and prior to formation of the functional spliceosome." Tarn W.Y., Lee K.R., Cheng S.C. Mol. Cell. Biol. 13:1883-1891(1993) [PubMed: 7680101] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Functional association of essential splicing factor(s) with PRP19 in a protein complex." Tarn W.Y., Hsu C.H., Huang K.T., Chen H.R., Kao H.Y., Lee K.R., Cheng S.C. EMBO J. 13:2421-2431(1994) [PubMed: 8194532] [Abstract] Cited for: SELF-ASSOCIATION, IDENTIFICATION IN THE PRP19-ASSOCIATED COMPLEX.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Structural and functional analysis of essential pre-mRNA splicing factor Prp19p." Ohi M.D., Vander Kooi C.W., Rosenberg J.A., Ren L., Hirsch J.P., Chazin W.J., Walz T., Gould K.L. Mol. Cell. Biol. 25:451-460(2005) [PubMed: 15601865] [Abstract] Cited for: TETRAMERIZATION, ELECTRON MICROSCOPY OF THE TETRAMER, INTERACTION WITH CEF1.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-141, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-140 AND SER-141, MASS SPECTROMETRY.
[11]	"Structural insights into the U-box, a domain associated with multi-ubiquitination." Ohi M.D., Vander Kooi C.W., Rosenberg J.A., Chazin W.J., Gould K.L. Nat. Struct. Biol. 10:250-255(2003) [PubMed: 12627222] [Abstract] Cited for: STRUCTURE BY NMR OF 1-56.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L09721 Genomic DNA. Translation: AAA34912.1.
X99770 Genomic DNA. Translation: CAA68103.1.
Z73142 Genomic DNA. Translation: CAA97487.1.
BK006945 Genomic DNA. Translation: DAA09285.1.

PIR

S64787.

RefSeq

NP_013064.1. NM_001181856.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1N87	NMR	-	A	1-56	[»]
2BAY	X-ray	1.50	A/B/C/D/E/F	1-58	[»]
3LRV	X-ray	2.60	A	165-503	[»]

ProteinModelPortal

P32523.

SMR

P32523. Positions 1-57, 171-503.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1112N.

IntAct

P32523. 58 interactions.

MINT

MINT-632220.

STRING

P32523.

Proteomic databases

PeptideAtlas

P32523.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YLL036C; YLL036C; YLL036C.

GeneID

850623.

KEGG

sce:YLL036C.

NMPDR

fig|4932.3.peg.4055.

Organism-specific databases

CYGD

YLL036c.

SGD

S000003959. PRP19.

Phylogenomic databases

eggNOG

fuNOG06368.

OrthoDB

EOG4M0JB3.

Gene expression databases

ArrayExpress

P32523.

Genevestigator

P32523.

GermOnline

YLL036C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR013915. Pre-mRNA_splic_Prp19.
IPR003613. Ubox_domain.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR011046. WD40_repeat-like_dom.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]

Gene3D

G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.

K10599.

Pfam

PF08606. Prp19. 1 hit.
[Graphical view]

SMART

SM00504. Ubox. 1 hit.
[Graphical view]

SUPFAM

SSF50978. WD40_like. 1 hit.

PROSITE

PS00678. WD_REPEATS_1. False negative.
PS50082. WD_REPEATS_2. False negative.
PS50294. WD_REPEATS_REGION. False negative.
[Graphical view]

ProtoNet

Search...

Other

NextBio

966526.

Entry information

Entry name

PRP19_YEAST

Accession

Primary (citable) accession number: P32523
Secondary accession number(s): D6VXW9, Q07870

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents