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Protein

Pre-mRNA-processing factor 19

Gene

PRP19

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable ubiquitin-protein ligase involved in pre-mRNA splicing. Acts as a central component of the NTC complex (or PRP19-associated complex) that associates to the spliceosome to mediate conformational rearrangement or to stabilize the structure of the spliceosome after U4 snRNA dissociation, which leads to spliceosome maturation. It is also probably involved in DNA repair.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • DNA repair Source: UniProtKB-KW
  • generation of catalytic spliceosome for first transesterification step Source: SGD
  • protein K63-linked ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA repair, mRNA processing, mRNA splicing, Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-32139-MONOMER.
BRENDAi2.3.2.B10. 984.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-processing factor 19Curated (EC:6.3.2.-1 Publication)
Gene namesi
Name:PRP19
Synonyms:PSO4
Ordered Locus Names:YLL036C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLL036C.
SGDiS000003959. PRP19.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • nucleus Source: SGD
  • Prp19 complex Source: SGD
  • U2-type catalytic step 1 spliceosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000585871 – 503Pre-mRNA-processing factor 19Add BLAST503

Proteomic databases

MaxQBiP32523.
PRIDEiP32523.

PTM databases

iPTMnetiP32523.

Interactioni

Subunit structurei

Homotetramer. Component of the NTC complex (or PRP19-associated complex), composed of at least CEF1, CLF1, ISY1, NTC20, SNT309, SYF1, SYF2, and PRP19. The NTC complex associates with the spliceosome after the release of the U1 and U4 snRNAs and forms the CWC spliceosome subcomplex (or CEF1-associated complex) reminiscent of a late-stage spliceosome composed also of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, LEA1, MSL1, PRP8, PRP9, PRP11, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNU114, SPP2, RSE1 and YJU2. Interacts with CLF1, ISY1, NTC20, PRP19, PRP46, SYF1 and SYF2.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC40P409687EBI-493,EBI-13834
CEF1Q036548EBI-493,EBI-476
CLF1Q123099EBI-493,EBI-484
CWC2Q120469EBI-493,EBI-553
ISY1P213746EBI-493,EBI-9382
NTC20P383025EBI-493,EBI-20921
SNT309Q060917EBI-493,EBI-818
SYF1Q040486EBI-493,EBI-540
SYF2P532774EBI-493,EBI-23308
URN1Q065257EBI-493,EBI-35138
USB1Q122083EBI-493,EBI-31589

Protein-protein interaction databases

BioGridi31217. 163 interactors.
DIPiDIP-1112N.
IntActiP32523. 58 interactors.
MINTiMINT-632220.

Structurei

Secondary structure

1503
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni4 – 6Combined sources3
Beta strandi11 – 16Combined sources6
Turni17 – 20Combined sources4
Beta strandi21 – 24Combined sources4
Helixi25 – 35Combined sources11
Turni39 – 41Combined sources3
Helixi47 – 49Combined sources3
Helixi149 – 163Combined sources15
Beta strandi179 – 187Combined sources9
Beta strandi192 – 194Combined sources3
Beta strandi197 – 200Combined sources4
Beta strandi204 – 216Combined sources13
Beta strandi219 – 227Combined sources9
Beta strandi232 – 237Combined sources6
Helixi241 – 243Combined sources3
Beta strandi247 – 252Combined sources6
Turni253 – 255Combined sources3
Beta strandi256 – 261Combined sources6
Turni262 – 264Combined sources3
Beta strandi265 – 270Combined sources6
Turni271 – 274Combined sources4
Beta strandi275 – 281Combined sources7
Beta strandi288 – 292Combined sources5
Beta strandi295 – 298Combined sources4
Beta strandi301 – 306Combined sources6
Beta strandi311 – 319Combined sources9
Beta strandi321 – 325Combined sources5
Beta strandi335 – 338Combined sources4
Beta strandi342 – 348Combined sources7
Beta strandi354 – 358Combined sources5
Beta strandi377 – 382Combined sources6
Beta strandi386 – 401Combined sources16
Beta strandi409 – 411Combined sources3
Beta strandi424 – 428Combined sources5
Beta strandi432 – 439Combined sources8
Turni440 – 443Combined sources4
Beta strandi444 – 451Combined sources8
Turni452 – 455Combined sources4
Beta strandi456 – 459Combined sources4
Beta strandi476 – 482Combined sources7
Beta strandi485 – 491Combined sources7
Beta strandi493 – 502Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1N87NMR-A1-56[»]
2BAYX-ray1.50A/B/C/D/E/F1-58[»]
3LRVX-ray2.60A165-503[»]
4ZB4X-ray2.30A/B144-503[»]
5GM6electron microscopy3.50o/p/q/r1-503[»]
5GMKelectron microscopy3.40o/p/q/r1-503[»]
5LJ5electron microscopy3.80t/u/v/w1-503[»]
ProteinModelPortaliP32523.
SMRiP32523.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32523.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 71U-boxAdd BLAST71
Repeati328 – 367WD 1Add BLAST40
Repeati372 – 410WD 2Add BLAST39
Repeati418 – 458WD 3Add BLAST41

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni57 – 144Required for self-associationAdd BLAST88
Regioni76 – 134Interaction with CEF11 PublicationAdd BLAST59

Sequence similaritiesi

Belongs to the WD repeat PRP19 family.Curated
Contains 1 U-box domain.Curated
Contains 3 WD repeats.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

HOGENOMiHOG000115709.
InParanoidiP32523.
KOiK10599.
OrthoDBiEOG092C45P3.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR013915. Pre-mRNA_splic_Prp19.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR003613. Ubox_domain.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF08606. Prp19. 1 hit.
[Graphical view]
SMARTiSM00504. Ubox. 1 hit.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 1 hit.
PROSITEiPS51698. U_BOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32523-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLCAISGKVP RRPVLSPKSR TIFEKSLLEQ YVKDTGNDPI TNEPLSIEEI
60 70 80 90 100
VEIVPSAQQA SLTESTNSAT LKANYSIPNL LTSLQNEWDA IMLENFKLRS
110 120 130 140 150
TLDSLTKKLS TVMYERDAAK LVAAQLLMEK NEDSKDLPKS SQQAVAITRE
160 170 180 190 200
EFLQGLLQSS RDFVARGKLK APKWPILKNL ELLQAQNYSR NIKTFPYKEL
210 220 230 240 250
NKSMYYDKWV CMCRCEDGAL HFTQLKDSKT ITTITTPNPR TGGEHPAIIS
260 270 280 290 300
RGPCNRLLLL YPGNQITILD SKTNKVLREI EVDSANEIIY MYGHNEVNTE
310 320 330 340 350
YFIWADNRGT IGFQSYEDDS QYIVHSAKSD VEYSSGVLHK DSLLLALYSP
360 370 380 390 400
DGILDVYNLS SPDQASSRFP VDEEAKIKEV KFADNGYWMV VECDQTVVCF
410 420 430 440 450
DLRKDVGTLA YPTYTIPEFK TGTVTYDIDD SGKNMIAYSN ESNSLTIYKF
460 470 480 490 500
DKKTKNWTKD EESALCLQSD TADFTDMDVV CGDGGIAAIL KTNDSFNIVA

LTP
Length:503
Mass (Da):56,570
Last modified:October 1, 1996 - v2
Checksum:iA44D8C7DD6F64185
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti239Missing in AAA34912 (PubMed:8441419).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L09721 Genomic DNA. Translation: AAA34912.1.
X99770 Genomic DNA. Translation: CAA68103.1.
Z73142 Genomic DNA. Translation: CAA97487.1.
BK006945 Genomic DNA. Translation: DAA09285.1.
PIRiS64787.
RefSeqiNP_013064.1. NM_001181856.1.

Genome annotation databases

EnsemblFungiiYLL036C; YLL036C; YLL036C.
GeneIDi850623.
KEGGisce:YLL036C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L09721 Genomic DNA. Translation: AAA34912.1.
X99770 Genomic DNA. Translation: CAA68103.1.
Z73142 Genomic DNA. Translation: CAA97487.1.
BK006945 Genomic DNA. Translation: DAA09285.1.
PIRiS64787.
RefSeqiNP_013064.1. NM_001181856.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1N87NMR-A1-56[»]
2BAYX-ray1.50A/B/C/D/E/F1-58[»]
3LRVX-ray2.60A165-503[»]
4ZB4X-ray2.30A/B144-503[»]
5GM6electron microscopy3.50o/p/q/r1-503[»]
5GMKelectron microscopy3.40o/p/q/r1-503[»]
5LJ5electron microscopy3.80t/u/v/w1-503[»]
ProteinModelPortaliP32523.
SMRiP32523.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31217. 163 interactors.
DIPiDIP-1112N.
IntActiP32523. 58 interactors.
MINTiMINT-632220.

PTM databases

iPTMnetiP32523.

Proteomic databases

MaxQBiP32523.
PRIDEiP32523.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLL036C; YLL036C; YLL036C.
GeneIDi850623.
KEGGisce:YLL036C.

Organism-specific databases

EuPathDBiFungiDB:YLL036C.
SGDiS000003959. PRP19.

Phylogenomic databases

HOGENOMiHOG000115709.
InParanoidiP32523.
KOiK10599.
OrthoDBiEOG092C45P3.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciYEAST:G3O-32139-MONOMER.
BRENDAi2.3.2.B10. 984.

Miscellaneous databases

EvolutionaryTraceiP32523.
PROiP32523.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR013915. Pre-mRNA_splic_Prp19.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR003613. Ubox_domain.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF08606. Prp19. 1 hit.
[Graphical view]
SMARTiSM00504. Ubox. 1 hit.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 1 hit.
PROSITEiPS51698. U_BOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRP19_YEAST
AccessioniPrimary (citable) accession number: P32523
Secondary accession number(s): D6VXW9, Q07870
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 155 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 11700 molecules/cell in log phase SD medium.1 Publication
The tetramer is an elongated particle consisting of four globular WD40 domains held together by a central stalk.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.