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P32522

- PT309_YEAST

UniProt

P32522 - PT309_YEAST

Protein

Pentatricopeptide repeat-containing protein PET309, mitochondrial

Gene

PET309

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Required for initiation of translation of the COX1 coding region. Also involved in the stability of the intron containing transcript of COX1.

    GO - Molecular functioni

    1. translation regulator activity Source: SGD
    2. tRNA binding Source: UniProtKB

    GO - Biological processi

    1. aerobic respiration Source: SGD
    2. mitochondrial respiratory chain complex IV biogenesis Source: SGD
    3. positive regulation of mitochondrial translational initiation Source: SGD
    4. RNA metabolic process Source: SGD
    5. tRNA 3'-end processing Source: UniProtKB

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32220-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pentatricopeptide repeat-containing protein PET309, mitochondrial
    Gene namesi
    Name:PET309
    Ordered Locus Names:YLR067C
    ORF Names:L2189
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR067c.
    SGDiS000004057. PET309.

    Subcellular locationi

    GO - Cellular componenti

    1. extrinsic component of mitochondrial inner membrane Source: SGD
    2. integral component of membrane Source: SGD
    3. mitochondrial inner membrane Source: SGD
    4. mitochondrial matrix Source: UniProtKB

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 965Pentatricopeptide repeat-containing protein PET309, mitochondrialPRO_0000022179
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Proteomic databases

    MaxQBiP32522.
    PaxDbiP32522.
    PRIDEiP32522.

    Expressioni

    Gene expression databases

    GenevestigatoriP32522.

    Interactioni

    Protein-protein interaction databases

    BioGridi31341. 52 interactions.
    DIPiDIP-932N.
    IntActiP32522. 4 interactions.
    MINTiMINT-425941.
    STRINGi4932.YLR067C.

    Structurei

    3D structure databases

    ProteinModelPortaliP32522.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei237 – 25620HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati310 – 34435PPR 1Add
    BLAST
    Repeati345 – 37935PPR 2Add
    BLAST
    Repeati380 – 41435PPR 3Add
    BLAST
    Repeati415 – 45036PPR 4Add
    BLAST
    Repeati451 – 48636PPR 5Add
    BLAST
    Repeati487 – 52337PPR 6Add
    BLAST
    Repeati524 – 55835PPR 7Add
    BLAST
    Repeati559 – 59537PPR 8Add
    BLAST
    Repeati596 – 63035PPR 9Add
    BLAST
    Repeati680 – 71435PPR 10Add
    BLAST
    Repeati723 – 76341PPR 11Add
    BLAST
    Repeati774 – 80835PPR 12Add
    BLAST
    Repeati809 – 84436PPR 13Add
    BLAST

    Sequence similaritiesi

    Contains 13 PPR (pentatricopeptide) repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transit peptide, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG317111.
    HOGENOMiHOG000115753.
    KOiK17669.
    OMAiATHTIML.
    OrthoDBiEOG7HB5JJ.

    Family and domain databases

    InterProiIPR002885. Pentatricopeptide_repeat.
    [Graphical view]
    PROSITEiPS51375. PPR. 9 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32522-1 [UniParc]FASTAAdd to Basket

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    MKRCAPAVLR NYNYKKGIWS TGVPDHIRKL LRDKSTSPLC SQDERNLVSY    50
    FMARGSVPLK SVGSGLTKKA TTSITSNSAT TTFERQYLIK YLYRHQAYGN 100
    VIKIAQKFLY TTIGSQRLLK QDASLPELKK FLLSLLILQR GIQLDQAISD 150
    IIQRFLLTQK TMVIDLINSI FSRMVIMNMH EEAVYKWVKW MKLVNGHCEF 200
    TNYMENKIVL RNFLSFMRQS NVRPDYLSYL KAIQLTQGPA IASQFATTLL 250
    FLLTYIRKFS SAEAVWNYKC EHNLPIVSSD LTCILKTYCH MQKFNLVSST 300
    YWKYPDAQHD QNQFDYLLVA HSRLHNWDAL QQQFNALFGI GKLPSIQHYG 350
    ILMYTMARIG ELDSVNKLYT QLLRRGMIPT YAVLQSLLYA HYKVGDFAAC 400
    FSHFELFKKY DITPSTATHT IMLKVYRGLN DLDGAFRILK RLSEDPSVEI 450
    TEGHFALLIQ MCCKTTNHLI AQELFNLMTE HYNIQHTGKS ISALMDVYIE 500
    SNRPTEAIAL FEKHSKNLSW RDGLISVYNK AIKAYIGLRN ANKCEELFDK 550
    ITTSKLAVNS EFYKMMIKFL VTLNEDCETA LSIIDQLIKH SVIKVDATHF 600
    EIIMEAYDKE GYRDGIINLY KTMSQNKVPA NSKILYYILK AVAKKSLQNN 650
    EEIKETINMV EDIMENAANG TLDVTYNKLH PSVMAWPMRM IVKHDSPQRA 700
    LELYNRYNEL FFKKHDWISN NNKFVMMRSL LVLLAQIEQW KDFETLFAKY 750
    MDRIENIENL PSSTTPNIKL RSIFSGLFPY KVSQLIAMNK IDELPLLWKK 800
    LREKGFILDN ISWNSAVEAL FKDPRTLSYG MKIVDDTLIH GYNLIHKFRL 850
    LTKLSEDKTQ SSDKSWPTLK MKEKEPNKFQ PRLYLKSDTY NSIMRQLDTY 900
    LDGIDDLKTL EDQVRDFISN YKYFMKDYLL MPRSKINKWE QIEMRHLSYF 950
    KELRKSKRVL PVSKF 965
    Length:965
    Mass (Da):112,646
    Last modified:October 1, 1993 - v1
    Checksum:i3E4B016D933AD4C5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L06072 Genomic DNA. Translation: AAA34856.1.
    X94607 Genomic DNA. Translation: CAA64313.1.
    Z73239 Genomic DNA. Translation: CAA97623.1.
    Z73240 Genomic DNA. Translation: CAA97625.1.
    BK006945 Genomic DNA. Translation: DAA09384.1.
    PIRiS58393.
    RefSeqiNP_013168.1. NM_001181954.1.

    Genome annotation databases

    EnsemblFungiiYLR067C; YLR067C; YLR067C.
    GeneIDi850756.
    KEGGisce:YLR067C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L06072 Genomic DNA. Translation: AAA34856.1 .
    X94607 Genomic DNA. Translation: CAA64313.1 .
    Z73239 Genomic DNA. Translation: CAA97623.1 .
    Z73240 Genomic DNA. Translation: CAA97625.1 .
    BK006945 Genomic DNA. Translation: DAA09384.1 .
    PIRi S58393.
    RefSeqi NP_013168.1. NM_001181954.1.

    3D structure databases

    ProteinModelPortali P32522.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31341. 52 interactions.
    DIPi DIP-932N.
    IntActi P32522. 4 interactions.
    MINTi MINT-425941.
    STRINGi 4932.YLR067C.

    Proteomic databases

    MaxQBi P32522.
    PaxDbi P32522.
    PRIDEi P32522.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR067C ; YLR067C ; YLR067C .
    GeneIDi 850756.
    KEGGi sce:YLR067C.

    Organism-specific databases

    CYGDi YLR067c.
    SGDi S000004057. PET309.

    Phylogenomic databases

    eggNOGi NOG317111.
    HOGENOMi HOG000115753.
    KOi K17669.
    OMAi ATHTIML.
    OrthoDBi EOG7HB5JJ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32220-MONOMER.

    Miscellaneous databases

    NextBioi 966897.

    Gene expression databases

    Genevestigatori P32522.

    Family and domain databases

    InterProi IPR002885. Pentatricopeptide_repeat.
    [Graphical view ]
    PROSITEi PS51375. PPR. 9 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The product of the nuclear gene PET309 is required for translation of mature mRNA and stability or production of intron-containing RNAs derived from the mitochondrial COX1 locus of Saccharomyces cerevisiae."
      Manthey G.M., McEwen J.E.
      EMBO J. 14:4031-4043(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The Saccharomyces cerevisiae Pet309 protein is embedded in the mitochondrial inner membrane."
      Manthey G.M., Przybyla-Zawislak B.D., McEwen J.E.
      Eur. J. Biochem. 255:156-161(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPT309_YEAST
    AccessioniPrimary (citable) accession number: P32522
    Secondary accession number(s): D6VY68
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1160 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3