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P32522

- PT309_YEAST

UniProt

P32522 - PT309_YEAST

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Protein

Pentatricopeptide repeat-containing protein PET309, mitochondrial

Gene

PET309

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Required for initiation of translation of the COX1 coding region. Also involved in the stability of the intron containing transcript of COX1.

GO - Molecular functioni

  1. translation regulator activity Source: SGD
  2. tRNA binding Source: UniProtKB

GO - Biological processi

  1. aerobic respiration Source: SGD
  2. mitochondrial respiratory chain complex IV biogenesis Source: SGD
  3. positive regulation of mitochondrial translational initiation Source: SGD
  4. RNA metabolic process Source: SGD
  5. tRNA 3'-end processing Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-32220-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pentatricopeptide repeat-containing protein PET309, mitochondrial
Gene namesi
Name:PET309
Ordered Locus Names:YLR067C
ORF Names:L2189
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR067c.
SGDiS000004057. PET309.

Subcellular locationi

GO - Cellular componenti

  1. extrinsic component of mitochondrial inner membrane Source: SGD
  2. integral component of membrane Source: SGD
  3. mitochondrial inner membrane Source: SGD
  4. mitochondrial matrix Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 965Pentatricopeptide repeat-containing protein PET309, mitochondrialPRO_0000022179
Transit peptidei1 – ?MitochondrionSequence Analysis

Proteomic databases

MaxQBiP32522.
PaxDbiP32522.
PRIDEiP32522.

Expressioni

Gene expression databases

GenevestigatoriP32522.

Interactioni

Protein-protein interaction databases

BioGridi31341. 52 interactions.
DIPiDIP-932N.
IntActiP32522. 4 interactions.
MINTiMINT-425941.
STRINGi4932.YLR067C.

Structurei

3D structure databases

ProteinModelPortaliP32522.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei237 – 25620HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati310 – 34435PPR 1Add
BLAST
Repeati345 – 37935PPR 2Add
BLAST
Repeati380 – 41435PPR 3Add
BLAST
Repeati415 – 45036PPR 4Add
BLAST
Repeati451 – 48636PPR 5Add
BLAST
Repeati487 – 52337PPR 6Add
BLAST
Repeati524 – 55835PPR 7Add
BLAST
Repeati559 – 59537PPR 8Add
BLAST
Repeati596 – 63035PPR 9Add
BLAST
Repeati680 – 71435PPR 10Add
BLAST
Repeati723 – 76341PPR 11Add
BLAST
Repeati774 – 80835PPR 12Add
BLAST
Repeati809 – 84436PPR 13Add
BLAST

Sequence similaritiesi

Contains 13 PPR (pentatricopeptide) repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG317111.
HOGENOMiHOG000115753.
InParanoidiP32522.
KOiK17669.
OMAiATHTIML.
OrthoDBiEOG7HB5JJ.

Family and domain databases

InterProiIPR002885. Pentatricopeptide_repeat.
[Graphical view]
PROSITEiPS51375. PPR. 9 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32522-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKRCAPAVLR NYNYKKGIWS TGVPDHIRKL LRDKSTSPLC SQDERNLVSY
60 70 80 90 100
FMARGSVPLK SVGSGLTKKA TTSITSNSAT TTFERQYLIK YLYRHQAYGN
110 120 130 140 150
VIKIAQKFLY TTIGSQRLLK QDASLPELKK FLLSLLILQR GIQLDQAISD
160 170 180 190 200
IIQRFLLTQK TMVIDLINSI FSRMVIMNMH EEAVYKWVKW MKLVNGHCEF
210 220 230 240 250
TNYMENKIVL RNFLSFMRQS NVRPDYLSYL KAIQLTQGPA IASQFATTLL
260 270 280 290 300
FLLTYIRKFS SAEAVWNYKC EHNLPIVSSD LTCILKTYCH MQKFNLVSST
310 320 330 340 350
YWKYPDAQHD QNQFDYLLVA HSRLHNWDAL QQQFNALFGI GKLPSIQHYG
360 370 380 390 400
ILMYTMARIG ELDSVNKLYT QLLRRGMIPT YAVLQSLLYA HYKVGDFAAC
410 420 430 440 450
FSHFELFKKY DITPSTATHT IMLKVYRGLN DLDGAFRILK RLSEDPSVEI
460 470 480 490 500
TEGHFALLIQ MCCKTTNHLI AQELFNLMTE HYNIQHTGKS ISALMDVYIE
510 520 530 540 550
SNRPTEAIAL FEKHSKNLSW RDGLISVYNK AIKAYIGLRN ANKCEELFDK
560 570 580 590 600
ITTSKLAVNS EFYKMMIKFL VTLNEDCETA LSIIDQLIKH SVIKVDATHF
610 620 630 640 650
EIIMEAYDKE GYRDGIINLY KTMSQNKVPA NSKILYYILK AVAKKSLQNN
660 670 680 690 700
EEIKETINMV EDIMENAANG TLDVTYNKLH PSVMAWPMRM IVKHDSPQRA
710 720 730 740 750
LELYNRYNEL FFKKHDWISN NNKFVMMRSL LVLLAQIEQW KDFETLFAKY
760 770 780 790 800
MDRIENIENL PSSTTPNIKL RSIFSGLFPY KVSQLIAMNK IDELPLLWKK
810 820 830 840 850
LREKGFILDN ISWNSAVEAL FKDPRTLSYG MKIVDDTLIH GYNLIHKFRL
860 870 880 890 900
LTKLSEDKTQ SSDKSWPTLK MKEKEPNKFQ PRLYLKSDTY NSIMRQLDTY
910 920 930 940 950
LDGIDDLKTL EDQVRDFISN YKYFMKDYLL MPRSKINKWE QIEMRHLSYF
960
KELRKSKRVL PVSKF
Length:965
Mass (Da):112,646
Last modified:October 1, 1993 - v1
Checksum:i3E4B016D933AD4C5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L06072 Genomic DNA. Translation: AAA34856.1.
X94607 Genomic DNA. Translation: CAA64313.1.
Z73239 Genomic DNA. Translation: CAA97623.1.
Z73240 Genomic DNA. Translation: CAA97625.1.
BK006945 Genomic DNA. Translation: DAA09384.1.
PIRiS58393.
RefSeqiNP_013168.1. NM_001181954.1.

Genome annotation databases

EnsemblFungiiYLR067C; YLR067C; YLR067C.
GeneIDi850756.
KEGGisce:YLR067C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L06072 Genomic DNA. Translation: AAA34856.1 .
X94607 Genomic DNA. Translation: CAA64313.1 .
Z73239 Genomic DNA. Translation: CAA97623.1 .
Z73240 Genomic DNA. Translation: CAA97625.1 .
BK006945 Genomic DNA. Translation: DAA09384.1 .
PIRi S58393.
RefSeqi NP_013168.1. NM_001181954.1.

3D structure databases

ProteinModelPortali P32522.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31341. 52 interactions.
DIPi DIP-932N.
IntActi P32522. 4 interactions.
MINTi MINT-425941.
STRINGi 4932.YLR067C.

Proteomic databases

MaxQBi P32522.
PaxDbi P32522.
PRIDEi P32522.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR067C ; YLR067C ; YLR067C .
GeneIDi 850756.
KEGGi sce:YLR067C.

Organism-specific databases

CYGDi YLR067c.
SGDi S000004057. PET309.

Phylogenomic databases

eggNOGi NOG317111.
HOGENOMi HOG000115753.
InParanoidi P32522.
KOi K17669.
OMAi ATHTIML.
OrthoDBi EOG7HB5JJ.

Enzyme and pathway databases

BioCyci YEAST:G3O-32220-MONOMER.

Miscellaneous databases

NextBioi 966897.

Gene expression databases

Genevestigatori P32522.

Family and domain databases

InterProi IPR002885. Pentatricopeptide_repeat.
[Graphical view ]
PROSITEi PS51375. PPR. 9 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The product of the nuclear gene PET309 is required for translation of mature mRNA and stability or production of intron-containing RNAs derived from the mitochondrial COX1 locus of Saccharomyces cerevisiae."
    Manthey G.M., McEwen J.E.
    EMBO J. 14:4031-4043(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The Saccharomyces cerevisiae Pet309 protein is embedded in the mitochondrial inner membrane."
    Manthey G.M., Przybyla-Zawislak B.D., McEwen J.E.
    Eur. J. Biochem. 255:156-161(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPT309_YEAST
AccessioniPrimary (citable) accession number: P32522
Secondary accession number(s): D6VY68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 29, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1160 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3