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P32522 (PT309_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pentatricopeptide repeat-containing protein PET309, mitochondrial
Gene names
Name:PET309
Ordered Locus Names:YLR067C
ORF Names:L2189
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length965 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for initiation of translation of the COX1 coding region. Also involved in the stability of the intron containing transcript of COX1.

Subcellular location

Mitochondrion inner membrane; Single-pass membrane protein Potential Ref.4.

Miscellaneous

Present with 1160 molecules/cell in log phase SD medium.

Sequence similarities

Contains 13 PPR (pentatricopeptide) repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 965Pentatricopeptide repeat-containing protein PET309, mitochondrialPRO_0000022179

Regions

Transmembrane237 – 25620Helical; Potential
Repeat310 – 34435PPR 1
Repeat345 – 37935PPR 2
Repeat380 – 41435PPR 3
Repeat415 – 45036PPR 4
Repeat451 – 48636PPR 5
Repeat487 – 52337PPR 6
Repeat524 – 55835PPR 7
Repeat559 – 59537PPR 8
Repeat596 – 63035PPR 9
Repeat680 – 71435PPR 10
Repeat723 – 76341PPR 11
Repeat774 – 80835PPR 12
Repeat809 – 84436PPR 13

Sequences

Sequence LengthMass (Da)Tools
P32522 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 3E4B016D933AD4C5

FASTA965112,646
        10         20         30         40         50         60 
MKRCAPAVLR NYNYKKGIWS TGVPDHIRKL LRDKSTSPLC SQDERNLVSY FMARGSVPLK 

        70         80         90        100        110        120 
SVGSGLTKKA TTSITSNSAT TTFERQYLIK YLYRHQAYGN VIKIAQKFLY TTIGSQRLLK 

       130        140        150        160        170        180 
QDASLPELKK FLLSLLILQR GIQLDQAISD IIQRFLLTQK TMVIDLINSI FSRMVIMNMH 

       190        200        210        220        230        240 
EEAVYKWVKW MKLVNGHCEF TNYMENKIVL RNFLSFMRQS NVRPDYLSYL KAIQLTQGPA 

       250        260        270        280        290        300 
IASQFATTLL FLLTYIRKFS SAEAVWNYKC EHNLPIVSSD LTCILKTYCH MQKFNLVSST 

       310        320        330        340        350        360 
YWKYPDAQHD QNQFDYLLVA HSRLHNWDAL QQQFNALFGI GKLPSIQHYG ILMYTMARIG 

       370        380        390        400        410        420 
ELDSVNKLYT QLLRRGMIPT YAVLQSLLYA HYKVGDFAAC FSHFELFKKY DITPSTATHT 

       430        440        450        460        470        480 
IMLKVYRGLN DLDGAFRILK RLSEDPSVEI TEGHFALLIQ MCCKTTNHLI AQELFNLMTE 

       490        500        510        520        530        540 
HYNIQHTGKS ISALMDVYIE SNRPTEAIAL FEKHSKNLSW RDGLISVYNK AIKAYIGLRN 

       550        560        570        580        590        600 
ANKCEELFDK ITTSKLAVNS EFYKMMIKFL VTLNEDCETA LSIIDQLIKH SVIKVDATHF 

       610        620        630        640        650        660 
EIIMEAYDKE GYRDGIINLY KTMSQNKVPA NSKILYYILK AVAKKSLQNN EEIKETINMV 

       670        680        690        700        710        720 
EDIMENAANG TLDVTYNKLH PSVMAWPMRM IVKHDSPQRA LELYNRYNEL FFKKHDWISN 

       730        740        750        760        770        780 
NNKFVMMRSL LVLLAQIEQW KDFETLFAKY MDRIENIENL PSSTTPNIKL RSIFSGLFPY 

       790        800        810        820        830        840 
KVSQLIAMNK IDELPLLWKK LREKGFILDN ISWNSAVEAL FKDPRTLSYG MKIVDDTLIH 

       850        860        870        880        890        900 
GYNLIHKFRL LTKLSEDKTQ SSDKSWPTLK MKEKEPNKFQ PRLYLKSDTY NSIMRQLDTY 

       910        920        930        940        950        960 
LDGIDDLKTL EDQVRDFISN YKYFMKDYLL MPRSKINKWE QIEMRHLSYF KELRKSKRVL 


PVSKF 

« Hide

References

« Hide 'large scale' references
[1]"The product of the nuclear gene PET309 is required for translation of mature mRNA and stability or production of intron-containing RNAs derived from the mitochondrial COX1 locus of Saccharomyces cerevisiae."
Manthey G.M., McEwen J.E.
EMBO J. 14:4031-4043(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The Saccharomyces cerevisiae Pet309 protein is embedded in the mitochondrial inner membrane."
Manthey G.M., Przybyla-Zawislak B.D., McEwen J.E.
Eur. J. Biochem. 255:156-161(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L06072 Genomic DNA. Translation: AAA34856.1.
X94607 Genomic DNA. Translation: CAA64313.1.
Z73239 Genomic DNA. Translation: CAA97623.1.
Z73240 Genomic DNA. Translation: CAA97625.1.
BK006945 Genomic DNA. Translation: DAA09384.1.
PIRS58393.
RefSeqNP_013168.1. NM_001181954.1.

3D structure databases

ProteinModelPortalP32522.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31341. 52 interactions.
DIPDIP-932N.
IntActP32522. 4 interactions.
MINTMINT-425941.
STRING4932.YLR067C.

Proteomic databases

PaxDbP32522.
PRIDEP32522.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR067C; YLR067C; YLR067C.
GeneID850756.
KEGGsce:YLR067C.

Organism-specific databases

CYGDYLR067c.
SGDS000004057. PET309.

Phylogenomic databases

eggNOGNOG317111.
HOGENOMHOG000115753.
KOK17669.
OMAEELFNIM.
OrthoDBEOG7HB5JJ.

Enzyme and pathway databases

BioCycYEAST:G3O-32220-MONOMER.

Gene expression databases

GenevestigatorP32522.

Family and domain databases

InterProIPR002885. Pentatricopeptide_repeat.
[Graphical view]
PROSITEPS51375. PPR. 9 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio966897.

Entry information

Entry namePT309_YEAST
AccessionPrimary (citable) accession number: P32522
Secondary accession number(s): D6VY68
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: April 16, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families