Reviewed, UniProtKB/Swiss-Prot P32522 (PT309_YEAST)

Names and origin

Protein names

Recommended name:
    Pentatricopeptide repeat-containing protein PET309, mitochondrial

Gene names

Name:	PET309
Ordered Locus Names:	YLR067C
ORF Names:	L2189

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	965 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for initiation of translation of the COX1 coding region. Also involved in the stability of the intron containing transcript of COX1.
Subcellular location	Mitochondrion inner membrane; Single-pass membrane protein Potential Ref.3.
Miscellaneous	Present with 1160 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Contains 13 PPR (pentatricopeptide) repeats.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Repeat Transit peptide Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA metabolic process Ref.1 Inferred from mutant phenotype. Source: SGD aerobic respiration Ref.1 Inferred from mutant phenotype. Source: SGD positive regulation of mitochondrial translational initiation Ref.1 Inferred from mutant phenotype. Source: SGD
Cellular component	extrinsic to mitochondrial inner membrane Inferred from direct assay. Source: SGD integral to membrane Ref.3 Inferred from direct assay. Source: SGD
Molecular function	translation regulator activity Ref.1 Inferred from genetic interaction. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 965		Pentatricopeptide repeat-containing protein PET309, mitochondrial		PRO_0000022179
Regions
	Transmembrane	237 – 256	20	Potential
	Repeat	310 – 344	35	PPR 1
	Repeat	345 – 379	35	PPR 2
	Repeat	380 – 414	35	PPR 3
	Repeat	415 – 450	36	PPR 4
	Repeat	451 – 486	36	PPR 5
	Repeat	487 – 523	37	PPR 6
	Repeat	524 – 558	35	PPR 7
	Repeat	559 – 595	37	PPR 8
	Repeat	596 – 630	35	PPR 9
	Repeat	680 – 714	35	PPR 10
	Repeat	723 – 763	41	PPR 11
	Repeat	774 – 808	35	PPR 12
	Repeat	809 – 844	36	PPR 13

Sequences

	Sequence		Length	Mass (Da)
	P32522-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 3E4B016D933AD4C5	FASTA	965	112,646
	10 20 30 40 50 60 MKRCAPAVLR NYNYKKGIWS TGVPDHIRKL LRDKSTSPLC SQDERNLVSY FMARGSVPLK 70 80 90 100 110 120 SVGSGLTKKA TTSITSNSAT TTFERQYLIK YLYRHQAYGN VIKIAQKFLY TTIGSQRLLK 130 140 150 160 170 180 QDASLPELKK FLLSLLILQR GIQLDQAISD IIQRFLLTQK TMVIDLINSI FSRMVIMNMH 190 200 210 220 230 240 EEAVYKWVKW MKLVNGHCEF TNYMENKIVL RNFLSFMRQS NVRPDYLSYL KAIQLTQGPA 250 260 270 280 290 300 IASQFATTLL FLLTYIRKFS SAEAVWNYKC EHNLPIVSSD LTCILKTYCH MQKFNLVSST 310 320 330 340 350 360 YWKYPDAQHD QNQFDYLLVA HSRLHNWDAL QQQFNALFGI GKLPSIQHYG ILMYTMARIG 370 380 390 400 410 420 ELDSVNKLYT QLLRRGMIPT YAVLQSLLYA HYKVGDFAAC FSHFELFKKY DITPSTATHT 430 440 450 460 470 480 IMLKVYRGLN DLDGAFRILK RLSEDPSVEI TEGHFALLIQ MCCKTTNHLI AQELFNLMTE 490 500 510 520 530 540 HYNIQHTGKS ISALMDVYIE SNRPTEAIAL FEKHSKNLSW RDGLISVYNK AIKAYIGLRN 550 560 570 580 590 600 ANKCEELFDK ITTSKLAVNS EFYKMMIKFL VTLNEDCETA LSIIDQLIKH SVIKVDATHF 610 620 630 640 650 660 EIIMEAYDKE GYRDGIINLY KTMSQNKVPA NSKILYYILK AVAKKSLQNN EEIKETINMV 670 680 690 700 710 720 EDIMENAANG TLDVTYNKLH PSVMAWPMRM IVKHDSPQRA LELYNRYNEL FFKKHDWISN 730 740 750 760 770 780 NNKFVMMRSL LVLLAQIEQW KDFETLFAKY MDRIENIENL PSSTTPNIKL RSIFSGLFPY 790 800 810 820 830 840 KVSQLIAMNK IDELPLLWKK LREKGFILDN ISWNSAVEAL FKDPRTLSYG MKIVDDTLIH 850 860 870 880 890 900 GYNLIHKFRL LTKLSEDKTQ SSDKSWPTLK MKEKEPNKFQ PRLYLKSDTY NSIMRQLDTY 910 920 930 940 950 960 LDGIDDLKTL EDQVRDFISN YKYFMKDYLL MPRSKINKWE QIEMRHLSYF KELRKSKRVL PVSKF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The product of the nuclear gene PET309 is required for translation of mature mRNA and stability or production of intron-containing RNAs derived from the mitochondrial COX1 locus of Saccharomyces cerevisiae." Manthey G.M., McEwen J.E. EMBO J. 14:4031-4043(1995) [PubMed: 7664742] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"The Saccharomyces cerevisiae Pet309 protein is embedded in the mitochondrial inner membrane." Manthey G.M., Przybyla-Zawislak B.D., McEwen J.E. Eur. J. Biochem. 255:156-161(1998) [PubMed: 9692914] [Abstract] Cited for: SUBCELLULAR LOCATION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L06072 Genomic DNA. Translation: AAA34856.1. X94607 Genomic DNA. Translation: CAA64313.1. Z73239 Genomic DNA. Translation: CAA97623.1. Z73240 Genomic DNA. Translation: CAA97625.1.
PIR	S58393.
RefSeq	NP_013168.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-932N.
STRING	P32522.
Genome annotation databases
Ensembl	YLR067C; YLR067C; YLR067C; Saccharomyces cerevisiae. [Genome view]
GeneID	850756.
KEGG	sce:YLR067C.
NMPDR	fig|4932.3.peg.4159.
Organism-specific databases
CYGD	YLR067c.
SGD	S000004057. PET309.
Phylogenomic databases
eggNOG	fuNOG08193.
OMA	EELFNIM.
OrthoDB	EOG9J3Z0N.
PhylomeDB	P32522.
Gene expression databases
ArrayExpress	P32522.
Genevestigator	P32522.
GermOnline	YLR067C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002885. Pentatricopeptide_repeat. [Graphical view]
TIGRFAMs	TIGR00756. PPR. 4 hits.
PROSITE	PS51375. PPR. 9 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	966897.

Entry information

Entry name

PT309_YEAST

Accession

Primary (citable) accession number: P32522

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	February 9, 2010
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)