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Protein

Actin cytoskeleton-regulatory complex protein PAN1

Gene

PAN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the PAN1 actin cytoskeleton-regulatory complex required for the internalization of endosomes during actin-coupled endocytosis. The complex links the site of endocytosis to the cell membrane-associated actin cytoskeleton. Mediates uptake of external molecules and vacuolar degradation of plasma membrane proteins. Plays a role in the proper organization of the cell membrane-associated actin cytoskeleton and promotes its destabilization. Required for the bipolar budding of diploid cells and the correct distribution of chitin at the cell surface.13 Publications

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • protein binding, bridging Source: SGD

GO - Biological processi

  • actin cortical patch assembly Source: SGD
  • axial cellular bud site selection Source: SGD
  • bipolar cellular bud site selection Source: SGD
  • endocytosis Source: SGD
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31427-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Actin cytoskeleton-regulatory complex protein PAN1
Alternative name(s):
Mitochondrial distribution of proteins protein 3
Gene namesi
Name:PAN1
Synonyms:DIM2, MDP3, MIP3
Ordered Locus Names:YIR006C
ORF Names:YIB6C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIR006C.
SGDiS000001445. PAN1.

Subcellular locationi

GO - Cellular componenti

  • actin cortical patch Source: SGD
  • cytoplasm Source: SGD
  • endosome membrane Source: UniProtKB-SubCell
  • nucleus Source: SGD
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000582211 – 1480Actin cytoskeleton-regulatory complex protein PAN1Add BLAST1480

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei241PhosphothreonineCombined sources1
Modified residuei570PhosphothreonineCombined sources1
Modified residuei747PhosphoserineCombined sources1
Modified residuei757PhosphoserineCombined sources1
Modified residuei993PhosphothreonineCombined sources1
Modified residuei995PhosphothreonineCombined sources1
Modified residuei1003PhosphoserineCombined sources1
Modified residuei1180PhosphoserineCombined sources1
Modified residuei1250PhosphoserineCombined sources1
Modified residuei1253PhosphoserineCombined sources1
Modified residuei1281PhosphoserineCombined sources1
Modified residuei1321PhosphothreonineCombined sources1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32521.
PRIDEiP32521.
TopDownProteomicsiP32521.

PTM databases

iPTMnetiP32521.

Interactioni

Subunit structurei

Forms homooligomers. Component of the PAN1 actin cytoskeleton-regulatory complex composed of at least END3, PAN1, and SLA1. Interacts directly with END3, and with ENT1, SCD5, SLA2, YAP1801 and YAP1802.10 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
END3P390139EBI-12875,EBI-6460
MYO5Q044392EBI-12875,EBI-11687
SLA1P327905EBI-12875,EBI-17313
YAP1801P388564EBI-12875,EBI-24811

GO - Molecular functioni

  • protein binding, bridging Source: SGD

Protein-protein interaction databases

BioGridi34997. 61 interactors.
DIPiDIP-1340N.
IntActiP32521. 22 interactors.
MINTiMINT-398969.

Structurei

3D structure databases

ProteinModelPortaliP32521.
SMRiP32521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati142 – 1531-1Add BLAST12
Repeati164 – 1751-2Add BLAST12
Repeati188 – 1991-3Add BLAST12
Repeati215 – 2261-4Add BLAST12
Repeati235 – 2461-5Add BLAST12
Domaini270 – 359EH 1PROSITE-ProRule annotationAdd BLAST90
Repeati328 – 3502-1Add BLAST23
Repeati392 – 4031-6Add BLAST12
Repeati409 – 4201-7Add BLAST12
Repeati422 – 4331-8Add BLAST12
Repeati446 – 4571-9Add BLAST12
Repeati467 – 4781-10Add BLAST12
Repeati498 – 5091-11Add BLAST12
Repeati510 – 5181-129
Repeati538 – 5481-13Add BLAST11
Repeati549 – 5561-148
Repeati564 – 5751-15Add BLAST12
Domaini600 – 689EH 2PROSITE-ProRule annotationAdd BLAST90
Repeati658 – 6802-2Add BLAST23
Repeati1084 – 10893-16
Repeati1090 – 10953-26
Repeati1096 – 11013-36
Repeati1102 – 11073-46
Repeati1108 – 11133-56
Repeati1114 – 11193-66
Repeati1120 – 11253-76
Repeati1315 – 13204-16
Repeati1321 – 13264-26
Repeati1327 – 13324-36
Repeati1340 – 13454-46
Repeati1346 – 13504-55
Repeati1355 – 13604-66
Repeati1361 – 13664-76
Repeati1372 – 13774-86

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni142 – 57515 X 12 AA tandem repeats of [SPNAG]-[IL]-[QKNGT]-[PSA]-[QT]-[GQAPISTLYK]-T-G-[YFGML]-[YVMGAQL]-[QVLNPAG]-[ASQPN]Add BLAST434
Regioni328 – 6802 X 23 AA repeats of F-A-L-[AG]-M-H-L-[IV]-[NY]-[DG]-[VK]-L-[QN]-G-[DK]-[TP]-I-P-[YN]-[EV]-L-[DP]-SAdd BLAST353
Regioni1084 – 11257 X 6 AA tandem repeats of Q-[PS]-T-Q-P-VAdd BLAST42
Regioni1315 – 13778 X 6 AA repeats of [ATVSP]-P-[LVI]-P-[SPQILA]-[VAS]Add BLAST63

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1131 – 1190Sequence analysisAdd BLAST60

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi13 – 22Poly-Gln10
Compositional biasi29 – 34Poly-Gln6
Compositional biasi98 – 106Poly-Gln9
Compositional biasi1400 – 1406Poly-Pro7
Compositional biasi1452 – 1455Poly-Glu4
Compositional biasi1474 – 1480Poly-Pro7

Sequence similaritiesi

Belongs to the PAN1 family.Curated
Contains 2 EH domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

GeneTreeiENSGT00730000114400.
InParanoidiP32521.
KOiK20047.
OMAiDDWSVVD.
OrthoDBiEOG092C25IB.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR013182. DUF1720.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000261. EH_dom.
IPR026812. Pan1.
[Graphical view]
PANTHERiPTHR11216:SF79. PTHR11216:SF79. 6 hits.
PfamiPF08226. DUF1720. 2 hits.
PF12763. EF-hand_4. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
SM00027. EH. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 2 hits.
PROSITEiPS50222. EF_HAND_2. 3 hits.
PS50031. EH. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32521-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNPYQQQGM GYQQQQQQQQ QQPNGFYPQQ QQGQSSNQPQ GQPQPQQQMA
60 70 80 90 100
FNQPQATGIG GMPQSFGNSF SSMPQQPQTG YNNNGNNGSV YGNGNFGQQP
110 120 130 140 150
QQQQQQAKPQ HTGYVPNSSM PMMNTTGTMP PPNPAQQPQL QSIQPQGTGY
160 170 180 190 200
YQAANTANVH SVQPLQSQGT GYYVSTPNLI SSNQTQQPLQ AQGTGYYQSQ
210 220 230 240 250
PQQVPPPQQA QSLQPLKPQQ TGFYLQPQNQ APLEPLKPTA TGFVNSFANN
260 270 280 290 300
GLNNDIKIPA IRLSFITAQD QAKFETLFRS IVTNGSNTVS GANCRKILMR
310 320 330 340 350
SGLPPSQLAR IWTLCDTSKA GELLFPEFAL AMHLINDVLQ GDTIPYELDS
360 370 380 390 400
KTKNEVSSFI DAINLSIANQ DSSANDAPKT PFDEFITAGV QNLQPQPTGY
410 420 430 440 450
MPQTSFGIPL QSQITGGGVA SALNPQSTGF MAPTTFNMSM NTGTPGLNPQ
460 470 480 490 500
ITGGAPASMQ PNITGNALQP QTTGMMPQTT GMMPQTTGMM PQTSFGVNLG
510 520 530 540 550
PQLTGGALQS QYTGGYGSVM PQQSGPASMP NLSFNQQGLQ SQLTGLQPQP
560 570 580 590 600
TGFLPPSNFS ATMPLTAQKT GFGNNEIYTK SNFNNNLIDN SSQDKISTEE
610 620 630 640 650
KSLFYKIFET FDTQNKGLLD SPTAVEIFRK SGLNRADLEQ IWNLCDINNT
660 670 680 690 700
GQLNKQEFAL GMHLVYGKLN GKPIPNVLPS SLIPSSTKLL DNLKNQLKTE
710 720 730 740 750
PTTTKEKPSF GKIDALSYKN NDDDVLPNYR NRRKVYSAKN EEQSSFSSPS
760 770 780 790 800
AKSVNHSSST LQTDDISVDK TVEKKTAKPK YAGFSREINL KNIASLENEI
810 820 830 840 850
KNISNPENCY DSSIPSDLTS RFDAIIAKLP NLFNEISTID NEITNAKIQL
860 870 880 890 900
YRKKNPSSII GSGPNGEITE NDRKKAKSRA LLRARMSALT GKSTESEDSL
910 920 930 940 950
SMEDEQQSAE IKRIQQENGK NQEIIKDIRS SISDISASLK STMTGSNMIS
960 970 980 990 1000
NQEFERWEFG IGLEDGVREF LDDLKSNSNK SVTESSPFVP SSTPTPVDDR
1010 1020 1030 1040 1050
SSSPSYSQFK TAEERAAYLK EQAKKRMKEK LAKFDKNRRN VTQSSRSISS
1060 1070 1080 1090 1100
ENSREQPQQI AGSSNLVEPR ATPFQEEKYV EVAQPTQPVQ STQPVQPTQP
1110 1120 1130 1140 1150
VQPTQPVQPT QPVQPTQPVQ PTQPVQNVYN AKQESDDEDE DDEEKRLQEE
1160 1170 1180 1190 1200
LKRLKLKKKA DKEKRLAALR KQIEDAQNES DEEETNGKDN FGGHVNVPQA
1210 1220 1230 1240 1250
APVAPSAAFS QNSTNAPRSV HAAVTPAAGK NSTGLPSTTM GHNPYFKDAS
1260 1270 1280 1290 1300
ASSTSTFDAR AAEMQRRIQR GLDEDEDDGW SDEDESNNRV AVDNKVEEAK
1310 1320 1330 1340 1350
IGHPDHARAP PVTAAPLPSV TPVPPAVPVP QANTSNEKSS PIPIAPIPPS
1360 1370 1380 1390 1400
VTQEPPVPLA PPLPAVDGFQ EPPIPSAPAI ATAVQKSGSS TPALAGGVLP
1410 1420 1430 1440 1450
PPPPLPTQQA STSEPIIAHV DNYNGAEKGT GAYGSDSDDD VLSIPESVGT
1460 1470 1480
DEEEEGAQPV STAGIPSIPP AGIPPPPPLP
Length:1,480
Mass (Da):160,267
Last modified:February 1, 1995 - v2
Checksum:iF3518495FF759553
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti235P → T in AAA34841 (PubMed:1339314).Curated1
Sequence conflicti266 – 273ITAQDQAK → YYCPRSGKN in AAA34841 (PubMed:1339314).Curated8
Sequence conflicti474 – 487Missing AA sequence (PubMed:1339314).CuratedAdd BLAST14
Sequence conflicti653 – 657Missing in AAA34841 (PubMed:1339314).Curated5
Sequence conflicti1291A → R in AAA34841 (PubMed:1339314).Curated1
Sequence conflicti1396 – 1480GGVLP…PPPLP → EAFCLHPHLYQLNKLPLQNL LSLTLITTMVLKKARAHMDP ILMMTFYRFLNQLVQMKRKK GHNQFLLQVSHQFHLQVFLH PHPFHEDLICFL in AAA34841 (PubMed:1339314).CuratedAdd BLAST85

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38062 Genomic DNA. Translation: CAA86208.1.
X79743 Genomic DNA. Translation: CAB38097.1.
M90688 Genomic DNA. Translation: AAA34841.1.
BK006942 Genomic DNA. Translation: DAA08552.1.
PIRiS48440.
RefSeqiNP_012271.3. NM_001179528.3.

Genome annotation databases

EnsemblFungiiYIR006C; YIR006C; YIR006C.
GeneIDi854822.
KEGGisce:YIR006C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38062 Genomic DNA. Translation: CAA86208.1.
X79743 Genomic DNA. Translation: CAB38097.1.
M90688 Genomic DNA. Translation: AAA34841.1.
BK006942 Genomic DNA. Translation: DAA08552.1.
PIRiS48440.
RefSeqiNP_012271.3. NM_001179528.3.

3D structure databases

ProteinModelPortaliP32521.
SMRiP32521.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34997. 61 interactors.
DIPiDIP-1340N.
IntActiP32521. 22 interactors.
MINTiMINT-398969.

PTM databases

iPTMnetiP32521.

Proteomic databases

MaxQBiP32521.
PRIDEiP32521.
TopDownProteomicsiP32521.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIR006C; YIR006C; YIR006C.
GeneIDi854822.
KEGGisce:YIR006C.

Organism-specific databases

EuPathDBiFungiDB:YIR006C.
SGDiS000001445. PAN1.

Phylogenomic databases

GeneTreeiENSGT00730000114400.
InParanoidiP32521.
KOiK20047.
OMAiDDWSVVD.
OrthoDBiEOG092C25IB.

Enzyme and pathway databases

BioCyciYEAST:G3O-31427-MONOMER.

Miscellaneous databases

PROiP32521.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR013182. DUF1720.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000261. EH_dom.
IPR026812. Pan1.
[Graphical view]
PANTHERiPTHR11216:SF79. PTHR11216:SF79. 6 hits.
PfamiPF08226. DUF1720. 2 hits.
PF12763. EF-hand_4. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
SM00027. EH. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 2 hits.
PROSITEiPS50222. EF_HAND_2. 3 hits.
PS50031. EH. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPAN1_YEAST
AccessioniPrimary (citable) accession number: P32521
Secondary accession number(s): D6VVT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 159 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a subunit of PAB-dependent poly(A)-specific ribonuclease.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.