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P32519 (ELF1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ETS-related transcription factor Elf-1
Alternative name(s):
E74-like factor 1
Gene names
Name:ELF1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length619 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer. Ref.8

Subunit structure

Binds to the underphosphorylated form of RB. May interact with other transcription factors in order to regulate specific genes. Interacts with RUNX1. Ref.7 Ref.10

Subcellular location

Nucleus.

Tissue specificity

In fetal tissues, it is highly expressed in heart, lung liver and kidney, and weakly expressed in brain. In adult, it is highly expressed in pancreas, spleen, thymus and peripheral blood leukocytes, expressed at moderate levels in heart, placenta, lung, liver, skeletal muscle, kidney, prostate, ovary, small intestine and colon, and weakly expressed in brain and testis. Ref.8 Ref.9

Sequence similarities

Belongs to the ETS family.

Contains 1 ETS DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   LigandDNA-binding
   Molecular functionActivator
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell differentiation

Inferred from Biological aspect of Ancestor. Source: RefGenome

negative regulation of T cell receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 14970218PubMed 19674970. Source: UniProtKB

positive regulation of transcription, DNA-templated

Inferred from direct assay PubMed 19674970. Source: UniProtKB

regulation of cytokine production

Inferred from electronic annotation. Source: Ensembl

transcription from RNA polymerase II promoter

Inferred from Biological aspect of Ancestor. Source: GOC

   Cellular_componentnucleus

Inferred from direct assay PubMed 19674970. Source: UniProtKB

   Molecular_functionprotein binding

Inferred from physical interaction Ref.10PubMed 19674970. Source: UniProtKB

sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding RNA polymerase II transcription factor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 14970218. Source: UniProtKB

Complete GO annotation...

Binary interactions

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P32519-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P32519-2)

The sequence of this isoform differs from the canonical sequence as follows:
     122-145: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 619619ETS-related transcription factor Elf-1
PRO_0000204085

Regions

DNA binding208 – 29083ETS
Compositional bias75 – 806Poly-Asp

Amino acid modifications

Modified residue1631Phosphoserine Ref.16
Modified residue1671Phosphoserine Ref.16
Modified residue1681Phosphoserine Ref.13
Modified residue4321Phosphoserine Ref.11 Ref.13 Ref.16

Natural variations

Alternative sequence122 – 14524Missing in isoform 2.
VSP_045682
Natural variant581N → S. Ref.2
Corresponds to variant rs7799 [ dbSNP | Ensembl ].
VAR_048942
Natural variant3431T → S. Ref.2
Corresponds to variant rs1056820 [ dbSNP | Ensembl ].
VAR_048943
Natural variant4031T → I.
Corresponds to variant rs7323148 [ dbSNP | Ensembl ].
VAR_048944

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 22, 2008. Version 2.
Checksum: FA0A51B3965616F3

FASTA61967,498
        10         20         30         40         50         60 
MAAVVQQNDL VFEFASNVME DERQLGDPAI FPAVIVEHVP GADILNSYAG LACVEEPNDM 

        70         80         90        100        110        120 
ITESSLDVAE EEIIDDDDDD ITLTVEASCH DGDETIETIE AAEALLNMDS PGPMLDEKRI 

       130        140        150        160        170        180 
NNNIFSSPED DMVVAPVTHV SVTLDGIPEV METQQVQEKY ADSPGASSPE QPKRKKGRKT 

       190        200        210        220        230        240 
KPPRPDSPAT TPNISVKKKN KDGKGNTIYL WEFLLALLQD KATCPKYIKW TQREKGIFKL 

       250        260        270        280        290        300 
VDSKAVSRLW GKHKNKPDMN YETMGRALRY YYQRGILAKV EGQRLVYQFK EMPKDLIYIN 

       310        320        330        340        350        360 
DEDPSSSIES SDPSLSSSAT SNRNQTSRSR VSSSPGVKGG ATTVLKPGNS KAAKPKDPVE 

       370        380        390        400        410        420 
VAQPSEVLRT VQPTQSPYPT QLFRTVHVVQ PVQAVPEGEA ARTSTMQDET LNSSVQSIRT 

       430        440        450        460        470        480 
IQAPTQVPVV VSPRNQQLHT VTLQTVPLTT VIASTDPSAG TGSQKFILQA IPSSQPMTVL 

       490        500        510        520        530        540 
KENVMLQSQK AGSPPSIVLG PAQVQQVLTS NVQTICNGTV SVASSPSFSA TAPVVTFSPR 

       550        560        570        580        590        600 
SSQLVAHPPG TVITSVIKTQ ETKTLTQEVE KKESEDHLKE NTEKTEQQPQ PYVMVVSSSN 

       610 
GFTSQVAMKQ NELLEPNSF 

« Hide

Isoform 2 [UniParc].

Checksum: CAF5EFA51EFAE710
Show »

FASTA59564,929

References

« Hide 'large scale' references
[1]"A novel Ets-related transcription factor, Elf-1, binds to human immunodeficiency virus type 2 regulatory elements that are required for inducible trans activation in T cells."
Leiden J.M., Wang C.Y., Petryniak B., Markovitz D.M., Nabel G.J., Thompson C.B.
J. Virol. 66:5890-5897(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS SER-58 AND SER-343.
Tissue: Trachea.
[3]"The DNA sequence and analysis of human chromosome 13."
Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Lymph.
[6]"Cis-acting sequences required for inducible interleukin-2 enhancer function bind a novel Ets-related protein, Elf-1."
Thompson C.B., Wang C.Y., Ho I.C., Bohjanen P.R., Petryniak B., June C.H., Miesfeldt S., Zhang L., Nabel G.J., Karpinski B.
Mol. Cell. Biol. 12:1043-1053(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 204-289 (ISOFORM 1).
[7]"Regulation of the Ets-related transcription factor Elf-1 by binding to the retinoblastoma protein."
Wang C.Y., Petryniak B., Thompson C.B., Kaelin W.G. Jr., Leiden J.M.
Science 260:1330-1335(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RB.
[8]"Characterization of NERF, a novel transcription factor related to the Ets factor ELF-1."
Oettgen P., Akbarali Y., Boltax J., Best J., Kunsch C., Libermann T.A.
Mol. Cell. Biol. 16:5091-5106(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[9]"Cloning of a novel human ELF-1-related ETS transcription factor, ELFR, its characterization and chromosomal assignment relative to ELF-1."
Aryee D.N.T., Petermann R., Kos K., Henn T., Haas O.A., Kovar H.
Gene 210:71-78(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[10]"Functional and physical interactions between AML1 proteins and an ETS protein, MEF: implications for the pathogenesis of t(8;21)-positive leukemias."
Mao S., Frank R.C., Zhang J., Miyazaki Y., Nimer S.D.
Mol. Cell. Biol. 19:3635-3644(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RUNX1.
[11]"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-432, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[12]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic kidney.
[13]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168 AND SER-432, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[14]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]"Large-scale proteomics analysis of the human kinome."
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H.
Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-163; SER-167 AND SER-432, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[17]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M82882 mRNA. No translation available.
AK304289 mRNA. Translation: BAG65147.1.
AL157877 Genomic DNA. Translation: CAI13220.1.
CH471075 Genomic DNA. Translation: EAX08640.1.
BC030507 mRNA. Translation: AAH30507.1.
CCDSCCDS45043.1. [P32519-2]
CCDS9374.1. [P32519-1]
PIRA43361.
RefSeqNP_001138825.1. NM_001145353.1. [P32519-2]
NP_758961.1. NM_172373.3. [P32519-1]
XP_005266333.1. XM_005266276.1. [P32519-1]
XP_005266334.1. XM_005266277.1. [P32519-1]
UniGeneHs.135646.

3D structure databases

ProteinModelPortalP32519.
SMRP32519. Positions 208-289.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid108312. 9 interactions.
DIPDIP-185N.
IntActP32519. 12 interactions.
MINTMINT-144425.
STRING9606.ENSP00000239882.

PTM databases

PhosphoSiteP32519.

Polymorphism databases

DMDM212288097.

Proteomic databases

MaxQBP32519.
PaxDbP32519.
PRIDEP32519.

Protocols and materials databases

DNASU1997.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000239882; ENSP00000239882; ENSG00000120690. [P32519-1]
ENST00000442101; ENSP00000405580; ENSG00000120690. [P32519-2]
GeneID1997.
KEGGhsa:1997.
UCSCuc001uxs.3. human. [P32519-1]

Organism-specific databases

CTD1997.
GeneCardsGC13M041506.
HGNCHGNC:3316. ELF1.
HPACAB005172.
HPA001755.
HPA048866.
MIM189973. gene.
neXtProtNX_P32519.
PharmGKBPA27744.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG291231.
HOGENOMHOG000049253.
HOVERGENHBG007183.
InParanoidP32519.
KOK09428.
OMATVEASCH.
OrthoDBEOG73RBD1.
PhylomeDBP32519.
TreeFamTF318679.

Gene expression databases

ArrayExpressP32519.
BgeeP32519.
CleanExHS_ELF1.
GenevestigatorP32519.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR000418. Ets_dom.
IPR022084. TF_Elf_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF12310. Elf-1_N. 1 hit.
PF00178. Ets. 1 hit.
[Graphical view]
PRINTSPR00454. ETSDOMAIN.
SMARTSM00413. ETS. 1 hit.
[Graphical view]
PROSITEPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSELF1. human.
GenomeRNAi1997.
NextBio8077.
PROP32519.
SOURCESearch...

Entry information

Entry nameELF1_HUMAN
AccessionPrimary (citable) accession number: P32519
Secondary accession number(s): B4E2I5 expand/collapse secondary AC list , E9PDQ9, Q8N6F6, Q9UDE1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: July 22, 2008
Last modified: July 9, 2014
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 13

Human chromosome 13: entries, gene names and cross-references to MIM