Reviewed,
UniProtKB/Swiss-Prot P32519 (ELF1_HUMAN)
Last modified
November 25, 2008.
Version 79.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: ETS-related transcription factor Elf-1 Alternative name(s): E74-like factor 1 | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 619 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer. |
| Subunit structure | Binds to the underphosphorylated form of RB. May interact with other transcription factors in order to regulate specific genes. Interacts with RUNX1. |
| Subcellular location | |
| Tissue specificity | In fetal tissue, it is highly expressed in heart, lung liver and kidney, and weakly expressed in brain. In adult, it is highly expressed in pancreas, spleen, thymus and peripheral blood leukocytes, expressed at moderate levels in heart, placenta, lung, liver, skeletal muscle, kidney, prostate, ovary, small intestine and colon, and weakly expressed in brain and testis. |
| Post-translational modification | Phosphorylated upon DNA damage, probably by ATM or ATR. |
| Sequence similarities | Belongs to the ETS family. Contains 1 ETS DNA-binding domain. |
Ontologies
Keywords | |
|---|---|
| Biological process | Transcription Transcription regulation |
| Cellular component | Nucleus |
| Ligand | DNA-binding |
| Molecular function | Activator |
| PTM | Phosphoprotein |
Gene Ontology (GO) | |
| Biological process | positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: UniProtKB |
| Cellular component | cytoplasm Inferred from direct assay. Source: HPA nucleusInferred by curator. Source: UniProtKB |
| Molecular function | protein binding Ref.9 Inferred from physical interaction. Source: UniProtKB sequence-specific DNA bindingInferred from electronic annotation. Source: InterPro transcription activator activity Ref.1Non-traceable author statement. Source: UniProtKB transcription factor activityInferred from direct assay. Source: UniProtKB |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 619 | 619 | ETS-related transcription factor Elf-1 | PRO_0000204085 | |||||
Regions | |||||||||
| DNA binding | 208 – 290 | 83 | ETS | ||||||
| Compositional bias | 75 – 80 | 6 | Poly-Asp | ||||||
Amino acid modifications | |||||||||
| Modified residue | 163 | 1 | Phosphoserine | ||||||
| Modified residue | 168 | 1 | Phosphoserine | ||||||
| Modified residue | 432 | 1 | Phosphoserine | ||||||
| Modified residue | 474 | 1 | Phosphoserine | ||||||
| Modified residue | 566 | 1 | Phosphothreonine | ||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "A novel Ets-related transcription factor, Elf-1, binds to human immunodeficiency virus type 2 regulatory elements that are required for inducible trans activation in T cells." Leiden J.M., Wang C.Y., Petryniak B., Markovitz D.M., Nabel G.J., Thompson C.B. J. Virol. 66:5890-5897(1992) [PubMed: 1527846] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. Ross M.T.Nature 428:522-528(2004) [PubMed: 15057823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [3] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph. |
| [5] | "Cis-acting sequences required for inducible interleukin-2 enhancer function bind a novel Ets-related protein, Elf-1." Thompson C.B., Wang C.Y., Ho I.C., Bohjanen P.R., Petryniak B., June C.H., Miesfeldt S., Zhang L., Nabel G.J., Karpinski B. Mol. Cell. Biol. 12:1043-1053(1992) [PubMed: 1545787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 204-289. |
| [6] | "Regulation of the Ets-related transcription factor Elf-1 by binding to the retinoblastoma protein." Wang C.Y., Petryniak B., Thompson C.B., Kaelin W.G. Jr., Leiden J.M. Science 260:1330-1335(1993) [PubMed: 8493578] [Abstract] Cited for: INTERACTION WITH RB. |
| [7] | "Characterization of NERF, a novel transcription factor related to the Ets factor ELF-1." Oettgen P., Akbarali Y., Boltax J., Best J., Kunsch C., Libermann T.A. Mol. Cell. Biol. 16:5091-5106(1996) [PubMed: 8756667] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY. |
| [8] | "Cloning of a novel human ELF-1-related ETS transcription factor, ELFR, its characterization and chromosomal assignment relative to ELF-1." Aryee D.N.T., Petermann R., Kos K., Henn T., Haas O.A., Kovar H. Gene 210:71-78(1998) [PubMed: 9524226] [Abstract] Cited for: TISSUE SPECIFICITY. |
| [9] | "Functional and physical interactions between AML1 proteins and an ETS protein, MEF: implications for the pathogenesis of t(8;21)-positive leukemias." Mao S., Frank R.C., Zhang J., Miyazaki Y., Nimer S.D. Mol. Cell. Biol. 19:3635-3644(1999) [PubMed: 10207087] [Abstract] Cited for: INTERACTION WITH RUNX1. |
| [10] | "Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168, MASS SPECTROMETRY. Tissue: Epithelium. |
| [11] | "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-432, MASS SPECTROMETRY. Tissue: Epithelium. |
| [12] | "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-474 AND THR-566, MASS SPECTROMETRY. |
| [13] | "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-432, MASS SPECTROMETRY. |
| [14] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-163; SER-168 AND SER-432, MASS SPECTROMETRY. |
Cross-references
Sequence databases | |
|---|---|
| M82882 mRNA. No translation available. AL157877 Genomic DNA. Translation: CAI13220.1. CH471075 Genomic DNA. Translation: EAX08640.1. BC030507 mRNA. Translation: AAH30507.1. | |
| PIR | A43361. |
| RefSeq | NP_758961.1. |
| UniGene | Hs.135646 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1BC8 based on UniProtKB P28324. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:185N. |
| IntAct | P32519. |
PTM databases | |
| PhosphoSite | P32519. |
Genome annotation databases | |
| Ensembl | ENSG00000120690. Homo sapiens. [Contig view] |
| GeneID | 1997. |
Organism-specific databases | |
| HGNC | HGNC:3316. ELF1. |
| HPA | CAB005172. HPA001755. |
| MIM | 189973. gene. |
| PharmGKB | PA27744. |
| GenAtlas | Search... |
| GeneCards | Search... |
Phylogenomic databases | |
| HOGENOM | P32519. |
| HOVERGEN | P32519. |
Gene expression databases | |
| ArrayExpress | P32519. |
| CleanEx | HS_ELF1. |
| GermOnline | ENSG00000120690. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR000418. Ets. IPR011991. Wing_hlx_DNA_bd. [Graphical view] |
| Gene3D | G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit. |
| Pfam | PF00178. Ets. 1 hit. [Graphical view] |
| PRINTS | PR00454. ETSDOMAIN. |
| SMART | SM00413. ETS. 1 hit. [Graphical view] |
| PROSITE | PS00345. ETS_DOMAIN_1. 1 hit. PS00346. ETS_DOMAIN_2. 1 hit. PS50061. ETS_DOMAIN_3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| LinkHub | P32519. |
| NextBio | 8077. |
| SOURCE | Search... |
Entry information
| Entry name | ELF1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P32519 Secondary accession number(s): Q8N6F6, Q9UDE1 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 13 Human chromosome 13: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


