Reviewed, UniProtKB/Swiss-Prot P32519 (ELF1_HUMAN)

Names and origin

Protein names

Recommended name:
    ETS-related transcription factor Elf-1
Alternative name(s):
    E74-like factor 1

Gene names

Name:

ELF1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer. Ref.7
Subunit structure	Binds to the underphosphorylated form of RB. May interact with other transcription factors in order to regulate specific genes. Interacts with RUNX1. Ref.6 Ref.9
Subcellular location	Nucleus.
Tissue specificity	In fetal tissue, it is highly expressed in heart, lung liver and kidney, and weakly expressed in brain. In adult, it is highly expressed in pancreas, spleen, thymus and peripheral blood leukocytes, expressed at moderate levels in heart, placenta, lung, liver, skeletal muscle, kidney, prostate, ovary, small intestine and colon, and weakly expressed in brain and testis. Ref.7 Ref.8
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.10 Ref.11 Ref.12 Ref.13 Ref.14
Sequence similarities	Belongs to the ETS family. Contains 1 ETS DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: HPA nucleus Inferred by curator. Source: UniProtKB
Molecular function	protein binding Ref.9 Inferred from physical interaction. Source: UniProtKB sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription activator activity Ref.1 Non-traceable author statement. Source: UniProtKB transcription factor activity Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SP1	P08047	1	EBI-765526,EBI-298336

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 619	619	ETS-related transcription factor Elf-1		PRO_0000204085
Regions
	DNA binding	208 – 290	83	ETS
	Compositional bias	75 – 80	6	Poly-Asp
Amino acid modifications
	Modified residue	163	1	Phosphoserine Ref.14
	Modified residue	168	1	Phosphoserine Ref.10 Ref.14
	Modified residue	432	1	Phosphoserine Ref.11 Ref.13 Ref.14
	Modified residue	474	1	Phosphoserine Ref.12
	Modified residue	566	1	Phosphothreonine Ref.12
Natural variations
	Natural variant	58	1	N → S: dbSNP rs7799.		VAR_048942
	Natural variant	343	1	T → S: dbSNP rs1056820.		VAR_048943
	Natural variant	403	1	T → I: dbSNP rs7323148.		VAR_048944

Sequences

	Sequence		Length	Mass (Da)
	P32519-1 [UniParc]. Last modified July 22, 2008. Version 2. Checksum: FA0A51B3965616F3	FASTA	619	67,498
	10 20 30 40 50 60 MAAVVQQNDL VFEFASNVME DERQLGDPAI FPAVIVEHVP GADILNSYAG LACVEEPNDM 70 80 90 100 110 120 ITESSLDVAE EEIIDDDDDD ITLTVEASCH DGDETIETIE AAEALLNMDS PGPMLDEKRI 130 140 150 160 170 180 NNNIFSSPED DMVVAPVTHV SVTLDGIPEV METQQVQEKY ADSPGASSPE QPKRKKGRKT 190 200 210 220 230 240 KPPRPDSPAT TPNISVKKKN KDGKGNTIYL WEFLLALLQD KATCPKYIKW TQREKGIFKL 250 260 270 280 290 300 VDSKAVSRLW GKHKNKPDMN YETMGRALRY YYQRGILAKV EGQRLVYQFK EMPKDLIYIN 310 320 330 340 350 360 DEDPSSSIES SDPSLSSSAT SNRNQTSRSR VSSSPGVKGG ATTVLKPGNS KAAKPKDPVE 370 380 390 400 410 420 VAQPSEVLRT VQPTQSPYPT QLFRTVHVVQ PVQAVPEGEA ARTSTMQDET LNSSVQSIRT 430 440 450 460 470 480 IQAPTQVPVV VSPRNQQLHT VTLQTVPLTT VIASTDPSAG TGSQKFILQA IPSSQPMTVL 490 500 510 520 530 540 KENVMLQSQK AGSPPSIVLG PAQVQQVLTS NVQTICNGTV SVASSPSFSA TAPVVTFSPR 550 560 570 580 590 600 SSQLVAHPPG TVITSVIKTQ ETKTLTQEVE KKESEDHLKE NTEKTEQQPQ PYVMVVSSSN 610 GFTSQVAMKQ NELLEPNSF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel Ets-related transcription factor, Elf-1, binds to human immunodeficiency virus type 2 regulatory elements that are required for inducible trans activation in T cells." Leiden J.M., Wang C.Y., Petryniak B., Markovitz D.M., Nabel G.J., Thompson C.B. J. Virol. 66:5890-5897(1992) [PubMed: 1527846] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed: 15057823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph.
[5]	"Cis-acting sequences required for inducible interleukin-2 enhancer function bind a novel Ets-related protein, Elf-1." Thompson C.B., Wang C.Y., Ho I.C., Bohjanen P.R., Petryniak B., June C.H., Miesfeldt S., Zhang L., Nabel G.J., Karpinski B. Mol. Cell. Biol. 12:1043-1053(1992) [PubMed: 1545787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 204-289.
[6]	"Regulation of the Ets-related transcription factor Elf-1 by binding to the retinoblastoma protein." Wang C.Y., Petryniak B., Thompson C.B., Kaelin W.G. Jr., Leiden J.M. Science 260:1330-1335(1993) [PubMed: 8493578] [Abstract] Cited for: INTERACTION WITH RB.
[7]	"Characterization of NERF, a novel transcription factor related to the Ets factor ELF-1." Oettgen P., Akbarali Y., Boltax J., Best J., Kunsch C., Libermann T.A. Mol. Cell. Biol. 16:5091-5106(1996) [PubMed: 8756667] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[8]	"Cloning of a novel human ELF-1-related ETS transcription factor, ELFR, its characterization and chromosomal assignment relative to ELF-1." Aryee D.N.T., Petermann R., Kos K., Henn T., Haas O.A., Kovar H. Gene 210:71-78(1998) [PubMed: 9524226] [Abstract] Cited for: TISSUE SPECIFICITY.
[9]	"Functional and physical interactions between AML1 proteins and an ETS protein, MEF: implications for the pathogenesis of t(8;21)-positive leukemias." Mao S., Frank R.C., Zhang J., Miyazaki Y., Nimer S.D. Mol. Cell. Biol. 19:3635-3644(1999) [PubMed: 10207087] [Abstract] Cited for: INTERACTION WITH RUNX1.
[10]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168, MASS SPECTROMETRY. Tissue: Epithelium.
[11]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-432, MASS SPECTROMETRY. Tissue: Epithelium.
[12]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-474 AND THR-566, MASS SPECTROMETRY.
[13]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-432, MASS SPECTROMETRY.
[14]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-163; SER-168 AND SER-432, MASS SPECTROMETRY.
[15]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M82882 mRNA. No translation available. AL157877 Genomic DNA. Translation: CAI13220.1. CH471075 Genomic DNA. Translation: EAX08640.1. BC030507 mRNA. Translation: AAH30507.1.
IPI	IPI00167002.
PIR	A43361.
RefSeq	NP_758961.1.
UniGene	Hs.135646
3D structure databases
HSSP	HSSP built from PDB template 1BC8 based on UniProtKB P28324.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:185N.
IntAct	P32519. 2 interactions.
PTM databases
PhosphoSite	P32519.
Proteomic databases
PRIDE	P32519.
Genome annotation databases
Ensembl	ENSG00000120690. Homo sapiens. [Contig view]
GeneID	1997.
KEGG	hsa:1997.
Organism-specific databases
GeneCards	GC13M040404.
HGNC	HGNC:3316. ELF1.
HPA	CAB005172. HPA001755.
MIM	189973. gene.
PharmGKB	PA27744.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P32519.
HOVERGEN	P32519.
OMA	P32519. ATCPKYI.
Enzyme and pathway databases
Pathway_Interaction_DB	angiopoietinreceptor_pathway. Angiopoietin receptor Tie2-mediated signaling. il2_stat5pathway. IL2 signaling events mediated by STAT5.
Gene expression databases
ArrayExpress	P32519.
Bgee	P32519.
CleanEx	HS_ELF1.
GermOnline	ENSG00000120690. Homo sapiens.
Family and domain databases
InterPro	IPR000418. Ets. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF00178. Ets. 1 hit. [Graphical view]
PRINTS	PR00454. ETSDOMAIN.
SMART	SM00413. ETS. 1 hit. [Graphical view]
PROSITE	PS00345. ETS_DOMAIN_1. 1 hit. PS00346. ETS_DOMAIN_2. 1 hit. PS50061. ETS_DOMAIN_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	8077.
SOURCE	Search...

Entry information

Entry name

ELF1_HUMAN

Accession

Primary (citable) accession number: P32519
Secondary accession number(s): Q8N6F6, Q9UDE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	July 22, 2008
Last modified:	June 16, 2009
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)