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P32518

- DUT_SOLLC

UniProt

P32518 - DUT_SOLLC

Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene
N/A
Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. It may have as well a metabolic role in merismatic cells.

    Catalytic activityi

    dUTP + H2O = dUMP + diphosphate.

    Cofactori

    Magnesium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei116 – 1161Substrate; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei159 – 1591SubstrateBy similarity

    GO - Molecular functioni

    1. dUTP diphosphatase activity Source: UniProtKB-EC
    2. magnesium ion binding Source: EnsemblPlants/Gramene

    GO - Biological processi

    1. DNA repair Source: EnsemblPlants/Gramene
    2. dUMP biosynthetic process Source: UniProtKB-UniPathway
    3. dUTP metabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Nucleotide metabolism

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00610; UER00666.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
    Short name:
    dUTPase
    Alternative name(s):
    P18
    dUTP pyrophosphatase
    OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
    Taxonomic identifieri4081 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
    ProteomesiUP000004994: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: EnsemblPlants/Gramene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 169169Deoxyuridine 5'-triphosphate nucleotidohydrolasePRO_0000182938Add
    BLAST

    Expressioni

    Tissue specificityi

    Vegetative and floral merismatic cells and provascular and vascular merismatic derivatives.

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliP32518.
    SMRiP32518. Positions 30-155.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni91 – 933Substrate bindingBy similarity
    Regioni105 – 1084Substrate bindingBy similarity
    Regioni164 – 1652Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the dUTPase family.Curated

    Phylogenomic databases

    KOiK01520.

    Family and domain databases

    Gene3Di2.70.40.10. 1 hit.
    InterProiIPR029054. dUTPase-like.
    IPR008180. dUTPase/dCTP_deaminase.
    IPR008181. dUTPase_1.
    [Graphical view]
    PfamiPF00692. dUTPase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51283. SSF51283. 1 hit.
    TIGRFAMsiTIGR00576. dut. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P32518-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAENQINSPE ITEPSPKVQK LDHPENGNVP FFRVKKLSEN AVLPSRASSL    50
    AAGYDLSSAA ETKVPARGKA LVPTDLSIAV PQGTYARIAP RSGLAWKYSI 100
    DVGAGVIDAD YRGPVGVVLF NHSEVDFEVK VGDRIAQLIV QKIVTPEVEQ 150
    VDDLDSTVRG SGGFGSTGV 169
    Length:169
    Mass (Da):17,929
    Last modified:October 1, 1993 - v1
    Checksum:i60FB80A052BBC224
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S40549 mRNA. Translation: AAB22611.1.
    PIRiJQ1599.
    RefSeqiNP_001233870.1. NM_001246941.1.
    UniGeneiLes.3597.

    Genome annotation databases

    GeneIDi544274.
    KEGGisly:544274.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S40549 mRNA. Translation: AAB22611.1 .
    PIRi JQ1599.
    RefSeqi NP_001233870.1. NM_001246941.1.
    UniGenei Les.3597.

    3D structure databases

    ProteinModelPortali P32518.
    SMRi P32518. Positions 30-155.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 544274.
    KEGGi sly:544274.

    Phylogenomic databases

    KOi K01520.

    Enzyme and pathway databases

    UniPathwayi UPA00610 ; UER00666 .

    Family and domain databases

    Gene3Di 2.70.40.10. 1 hit.
    InterProi IPR029054. dUTPase-like.
    IPR008180. dUTPase/dCTP_deaminase.
    IPR008181. dUTPase_1.
    [Graphical view ]
    Pfami PF00692. dUTPase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51283. SSF51283. 1 hit.
    TIGRFAMsi TIGR00576. dut. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A meristem-related gene from tomato encodes a dUTPase: analysis of expression in vegetative and floral meristems."
      Pri-Hadash A., Hareven D., Lifschitz E.
      Plant Cell 4:149-159(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Tiny Tim LA154.
      Tissue: Meristem.

    Entry informationi

    Entry nameiDUT_SOLLC
    AccessioniPrimary (citable) accession number: P32518
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3