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P32518

- DUT_SOLLC

UniProt

P32518 - DUT_SOLLC

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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene
N/A
Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. It may have as well a metabolic role in merismatic cells.

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.

Cofactori

Magnesium.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei116 – 1161Substrate; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei159 – 1591SubstrateBy similarity

GO - Molecular functioni

  1. dUTP diphosphatase activity Source: UniProtKB-EC
  2. magnesium ion binding Source: EnsemblPlants/Gramene

GO - Biological processi

  1. DNA-dependent DNA replication Source: EnsemblPlants/Gramene
  2. DNA repair Source: EnsemblPlants/Gramene
  3. dUMP biosynthetic process Source: UniProtKB-UniPathway
  4. dUTP metabolic process Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00610; UER00666.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
Short name:
dUTPase
Alternative name(s):
P18
dUTP pyrophosphatase
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
ProteomesiUP000004994: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: EnsemblPlants/Gramene
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 169169Deoxyuridine 5'-triphosphate nucleotidohydrolasePRO_0000182938Add
BLAST

Expressioni

Tissue specificityi

Vegetative and floral merismatic cells and provascular and vascular merismatic derivatives.

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP32518.
SMRiP32518. Positions 30-155.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni91 – 933Substrate bindingBy similarity
Regioni105 – 1084Substrate bindingBy similarity
Regioni164 – 1652Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the dUTPase family.Curated

Phylogenomic databases

KOiK01520.

Family and domain databases

Gene3Di2.70.40.10. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.

Sequencei

Sequence statusi: Complete.

P32518-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAENQINSPE ITEPSPKVQK LDHPENGNVP FFRVKKLSEN AVLPSRASSL
60 70 80 90 100
AAGYDLSSAA ETKVPARGKA LVPTDLSIAV PQGTYARIAP RSGLAWKYSI
110 120 130 140 150
DVGAGVIDAD YRGPVGVVLF NHSEVDFEVK VGDRIAQLIV QKIVTPEVEQ
160
VDDLDSTVRG SGGFGSTGV
Length:169
Mass (Da):17,929
Last modified:October 1, 1993 - v1
Checksum:i60FB80A052BBC224
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S40549 mRNA. Translation: AAB22611.1.
PIRiJQ1599.
RefSeqiNP_001233870.1. NM_001246941.1.
UniGeneiLes.3597.

Genome annotation databases

GeneIDi544274.
KEGGisly:544274.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S40549 mRNA. Translation: AAB22611.1 .
PIRi JQ1599.
RefSeqi NP_001233870.1. NM_001246941.1.
UniGenei Les.3597.

3D structure databases

ProteinModelPortali P32518.
SMRi P32518. Positions 30-155.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 544274.
KEGGi sly:544274.

Phylogenomic databases

KOi K01520.

Enzyme and pathway databases

UniPathwayi UPA00610 ; UER00666 .

Family and domain databases

Gene3Di 2.70.40.10. 1 hit.
InterProi IPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view ]
Pfami PF00692. dUTPase. 1 hit.
[Graphical view ]
SUPFAMi SSF51283. SSF51283. 1 hit.
TIGRFAMsi TIGR00576. dut. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "A meristem-related gene from tomato encodes a dUTPase: analysis of expression in vegetative and floral meristems."
    Pri-Hadash A., Hareven D., Lifschitz E.
    Plant Cell 4:149-159(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Tiny Tim LA154.
    Tissue: Meristem.

Entry informationi

Entry nameiDUT_SOLLC
AccessioniPrimary (citable) accession number: P32518
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 29, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3