P32518 (DUT_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Deoxyuridine 5'-triphosphate nucleotidohydrolase Short name=dUTPase EC=3.6.1.23 Alternative name(s): P18 dUTP pyrophosphatase
Organism	Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]
Taxonomic identifier	4081 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	169 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. It may have as well a metabolic role in merismatic cells.
Catalytic activity	dUTP + H2O = dUMP + diphosphate.
Cofactor	Magnesium By similarity.
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
Subunit structure	Homodimer.
Tissue specificity	Vegetative and floral merismatic cells and provascular and vascular merismatic derivatives.
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dUMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	dUTP diphosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 169	169	Deoxyuridine 5'-triphosphate nucleotidohydrolase		PRO_0000182938
Regions
	Region	91 – 93	3	Substrate binding By similarity
	Region	105 – 108	4	Substrate binding By similarity
	Region	164 – 165	2	Substrate binding By similarity
Sites
	Binding site	116	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	159	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32518 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 60FB80A052BBC224	FASTA	169	17,929
	10 20 30 40 50 60 MAENQINSPE ITEPSPKVQK LDHPENGNVP FFRVKKLSEN AVLPSRASSL AAGYDLSSAA 70 80 90 100 110 120 ETKVPARGKA LVPTDLSIAV PQGTYARIAP RSGLAWKYSI DVGAGVIDAD YRGPVGVVLF 130 140 150 160 NHSEVDFEVK VGDRIAQLIV QKIVTPEVEQ VDDLDSTVRG SGGFGSTGV

References

[1]

"A meristem-related gene from tomato encodes a dUTPase: analysis of expression in vegetative and floral meristems." Pri-Hadash A., Hareven D., Lifschitz E. Plant Cell 4:149-159(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Tiny Tim LA154. Tissue: Meristem.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S40549 mRNA. Translation: AAB22611.1.
PIR	JQ1599.
RefSeq	NP_001233870.1. NM_001246941.1.
UniGene	Les.3597.
3D structure databases
ProteinModelPortal	P32518.
SMR	P32518. Positions 30-155.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	544274.
Enzyme and pathway databases
UniPathway	UPA00610; UER00666.
Family and domain databases
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_SOLLC

Accession

Primary (citable) accession number: P32518

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	April 3, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program