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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene
N/A
Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. It may have as well a metabolic role in merismatic cells.

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.

Cofactori

Mg2+By similarity

Pathwayi: dUMP biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes dUMP from dCTP (dUTP route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Deoxyuridine 5'-triphosphate nucleotidohydrolase
This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei116Substrate; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei159SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00610; UER00666.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
Short name:
dUTPase
Alternative name(s):
P18
dUTP pyrophosphatase
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
Proteomesi
  • UP000004994 Componenti: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001829381 – 169Deoxyuridine 5'-triphosphate nucleotidohydrolaseAdd BLAST169

Proteomic databases

PaxDbiP32518.

Expressioni

Tissue specificityi

Vegetative and floral merismatic cells and provascular and vascular merismatic derivatives.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi4081.Solyc01g100030.2.1.

Structurei

3D structure databases

ProteinModelPortaliP32518.
SMRiP32518.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni91 – 93Substrate bindingBy similarity3
Regioni105 – 108Substrate bindingBy similarity4
Regioni164 – 165Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the dUTPase family.Curated

Phylogenomic databases

eggNOGiKOG3370. Eukaryota.
COG0756. LUCA.
KOiK01520.

Family and domain databases

CDDicd07557. trimeric_dUTPase. 1 hit.
InterProiView protein in InterPro
IPR029054. dUTPase-like.
IPR008181. dUTPase_1.
IPR033704. dUTPase_trimeric.
PfamiView protein in Pfam
PF00692. dUTPase. 1 hit.
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.

Sequencei

Sequence statusi: Complete.

P32518-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAENQINSPE ITEPSPKVQK LDHPENGNVP FFRVKKLSEN AVLPSRASSL
60 70 80 90 100
AAGYDLSSAA ETKVPARGKA LVPTDLSIAV PQGTYARIAP RSGLAWKYSI
110 120 130 140 150
DVGAGVIDAD YRGPVGVVLF NHSEVDFEVK VGDRIAQLIV QKIVTPEVEQ
160
VDDLDSTVRG SGGFGSTGV
Length:169
Mass (Da):17,929
Last modified:October 1, 1993 - v1
Checksum:i60FB80A052BBC224
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S40549 mRNA. Translation: AAB22611.1.
PIRiJQ1599.
RefSeqiNP_001233870.1. NM_001246941.2.
UniGeneiLes.3597.

Genome annotation databases

GeneIDi544274.
KEGGisly:544274.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiDUT_SOLLC
AccessioniPrimary (citable) accession number: P32518
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 5, 2017
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families