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P32518

- DUT_SOLLC

UniProt

P32518 - DUT_SOLLC

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Protein
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Gene
N/A
Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. It may have as well a metabolic role in merismatic cells.

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.

Cofactori

Magnesium By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei116 – 1161Substrate; via amide nitrogen and carbonyl oxygen By similarity
Binding sitei159 – 1591Substrate By similarity

GO - Molecular functioni

  1. dUTP diphosphatase activity Source: UniProtKB-EC
  2. magnesium ion binding Source: EnsemblPlants/Gramene
Complete GO annotation...

GO - Biological processi

  1. DNA repair Source: EnsemblPlants/Gramene
  2. dUMP biosynthetic process Source: UniProtKB-UniPathway
  3. dUTP metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00610; UER00666.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
Short name:
dUTPase
Alternative name(s):
P18
dUTP pyrophosphatase
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
ProteomesiUP000004994: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: EnsemblPlants/Gramene
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 169169Deoxyuridine 5'-triphosphate nucleotidohydrolase
PRO_0000182938Add
BLAST

Expressioni

Tissue specificityi

Vegetative and floral merismatic cells and provascular and vascular merismatic derivatives.

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP32518.
SMRiP32518. Positions 30-155.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni91 – 933Substrate binding By similarity
Regioni105 – 1084Substrate binding By similarity
Regioni164 – 1652Substrate binding By similarity

Sequence similaritiesi

Belongs to the dUTPase family.

Phylogenomic databases

KOiK01520.

Family and domain databases

Gene3Di2.70.40.10. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.

Sequencei

Sequence statusi: Complete.

P32518-1 [UniParc]FASTAAdd to Basket

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MAENQINSPE ITEPSPKVQK LDHPENGNVP FFRVKKLSEN AVLPSRASSL    50
AAGYDLSSAA ETKVPARGKA LVPTDLSIAV PQGTYARIAP RSGLAWKYSI 100
DVGAGVIDAD YRGPVGVVLF NHSEVDFEVK VGDRIAQLIV QKIVTPEVEQ 150
VDDLDSTVRG SGGFGSTGV 169
Length:169
Mass (Da):17,929
Last modified:October 1, 1993 - v1
Checksum:i60FB80A052BBC224
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S40549 mRNA. Translation: AAB22611.1.
PIRiJQ1599.
RefSeqiNP_001233870.1. NM_001246941.1.
UniGeneiLes.3597.

Genome annotation databases

GeneIDi544274.
KEGGisly:544274.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S40549 mRNA. Translation: AAB22611.1 .
PIRi JQ1599.
RefSeqi NP_001233870.1. NM_001246941.1.
UniGenei Les.3597.

3D structure databases

ProteinModelPortali P32518.
SMRi P32518. Positions 30-155.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 544274.
KEGGi sly:544274.

Phylogenomic databases

KOi K01520.

Enzyme and pathway databases

UniPathwayi UPA00610 ; UER00666 .

Family and domain databases

Gene3Di 2.70.40.10. 1 hit.
InterProi IPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view ]
Pfami PF00692. dUTPase. 1 hit.
[Graphical view ]
SUPFAMi SSF51283. SSF51283. 1 hit.
TIGRFAMsi TIGR00576. dut. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "A meristem-related gene from tomato encodes a dUTPase: analysis of expression in vegetative and floral meristems."
    Pri-Hadash A., Hareven D., Lifschitz E.
    Plant Cell 4:149-159(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Tiny Tim LA154.
    Tissue: Meristem.

Entry informationi

Entry nameiDUT_SOLLC
AccessioniPrimary (citable) accession number: P32518
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 11, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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