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P32518 (DUT_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase

Short name=dUTPase
EC=3.6.1.23
Alternative name(s):
P18
dUTP pyrophosphatase
OrganismSolanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]
Taxonomic identifier4081 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon

Protein attributes

Sequence length169 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. It may have as well a metabolic role in merismatic cells.

Catalytic activity

dUTP + H2O = dUMP + diphosphate.

Cofactor

Magnesium By similarity.

Pathway

Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.

Subunit structure

Homodimer.

Tissue specificity

Vegetative and floral merismatic cells and provascular and vascular merismatic derivatives.

Sequence similarities

Belongs to the dUTPase family.

Ontologies

Keywords
   Biological processNucleotide metabolism
   LigandMagnesium
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA repair

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

dUMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

dUTP metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytosol

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functiondUTP diphosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

magnesium ion binding

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 169169Deoxyuridine 5'-triphosphate nucleotidohydrolase
PRO_0000182938

Regions

Region91 – 933Substrate binding By similarity
Region105 – 1084Substrate binding By similarity
Region164 – 1652Substrate binding By similarity

Sites

Binding site1161Substrate; via amide nitrogen and carbonyl oxygen By similarity
Binding site1591Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P32518 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 60FB80A052BBC224

FASTA16917,929
        10         20         30         40         50         60 
MAENQINSPE ITEPSPKVQK LDHPENGNVP FFRVKKLSEN AVLPSRASSL AAGYDLSSAA 

        70         80         90        100        110        120 
ETKVPARGKA LVPTDLSIAV PQGTYARIAP RSGLAWKYSI DVGAGVIDAD YRGPVGVVLF 

       130        140        150        160 
NHSEVDFEVK VGDRIAQLIV QKIVTPEVEQ VDDLDSTVRG SGGFGSTGV 

« Hide

References

[1]"A meristem-related gene from tomato encodes a dUTPase: analysis of expression in vegetative and floral meristems."
Pri-Hadash A., Hareven D., Lifschitz E.
Plant Cell 4:149-159(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Tiny Tim LA154.
Tissue: Meristem.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S40549 mRNA. Translation: AAB22611.1.
PIRJQ1599.
RefSeqNP_001233870.1. NM_001246941.1.
UniGeneLes.3597.

3D structure databases

ProteinModelPortalP32518.
SMRP32518. Positions 30-155.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID544274.
KEGGsly:544274.

Phylogenomic databases

KOK01520.

Enzyme and pathway databases

UniPathwayUPA00610; UER00666.

Family and domain databases

InterProIPR008180. dUTP_pyroPase.
IPR008181. dUTP_pyroPase_sf.
[Graphical view]
PfamPF00692. dUTPase. 1 hit.
[Graphical view]
TIGRFAMsTIGR00576. dut. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDUT_SOLLC
AccessionPrimary (citable) accession number: P32518
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: March 19, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways