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Protein

Nectin-2

Gene

Nectin2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable cell adhesion protein.

GO - Molecular functioni

  • cell adhesion molecule binding Source: BHF-UCL
  • identical protein binding Source: MGI
  • protein homodimerization activity Source: HGNC
  • receptor activity Source: GO_Central
  • receptor binding Source: GO_Central

GO - Biological processi

  • acrosome assembly Source: MGI
  • adhesion of symbiont to host Source: MGI
  • cell part morphogenesis Source: MGI
  • cell recognition Source: GO_Central
  • cilium organization Source: MGI
  • coreceptor-mediated virion attachment to host cell Source: MGI
  • cytoskeleton organization Source: MGI
  • establishment of mitochondrion localization Source: MGI
  • fertilization Source: MGI
  • fusion of virus membrane with host plasma membrane Source: MGI
  • heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: GO_Central
  • homophilic cell adhesion via plasma membrane adhesion molecules Source: HGNC
  • positive regulation of immunoglobulin mediated immune response Source: MGI
  • positive regulation of mast cell activation Source: MGI
  • positive regulation of natural killer cell mediated cytotoxicity Source: MGI
  • positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target Source: MGI
  • regulation of viral entry into host cell Source: MGI
  • spermatid development Source: BHF-UCL
  • spermatid nucleus differentiation Source: MGI
  • sperm mitochondrion organization Source: MGI
  • susceptibility to natural killer cell mediated cytotoxicity Source: MGI
  • susceptibility to T cell mediated cytotoxicity Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-MMU-418990. Adherens junctions interactions.
R-MMU-420597. Nectin/Necl trans heterodimerization.

Names & Taxonomyi

Protein namesi
Recommended name:
Nectin-2
Alternative name(s):
Herpes virus entry mediator B
Short name:
Herpesvirus entry mediator B
Short name:
HveB
Murine herpes virus entry protein B
Short name:
mHveB
Nectin cell adhesion molecule 2By similarity
Poliovirus receptor homolog
Poliovirus receptor-related protein 2
CD_antigen: CD112
Gene namesi
Name:Nectin2By similarity
Synonyms:Mph, Pvr, Pvrl2, Pvs
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:97822. Nectin2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini32 – 351320ExtracellularSequence analysisAdd
BLAST
Transmembranei352 – 37221HelicalSequence analysisAdd
BLAST
Topological domaini373 – 530158CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell-cell junction Source: MGI
  • cell surface Source: MGI
  • extracellular exosome Source: MGI
  • focal adhesion Source: MGI
  • integral component of membrane Source: MGI
  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: HGNC
  • zonula adherens Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence analysisAdd
BLAST
Chaini32 – 530499Nectin-2PRO_0000015137Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi54 ↔ 131PROSITE-ProRule annotation1 Publication
Glycosylationi128 – 1281N-linked (GlcNAc...)2 Publications
Glycosylationi138 – 1381N-linked (GlcNAc...)2 Publications
Disulfide bondi174 ↔ 229PROSITE-ProRule annotation1 Publication
Disulfide bondi274 ↔ 320PROSITE-ProRule annotation
Glycosylationi315 – 3151N-linked (GlcNAc...)1 Publication
Modified residuei401 – 4011PhosphothreonineCombined sources
Modified residuei424 – 4241PhosphoserineCombined sources

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP32507.
PaxDbiP32507.
PeptideAtlasiP32507.
PRIDEiP32507.

PTM databases

iPTMnetiP32507.
PhosphoSiteiP32507.
SwissPalmiP32507.

Expressioni

Tissue specificityi

Brain, spinal cord, spleen, kidney, heart and liver.

Gene expression databases

BgeeiP32507.
CleanExiMM_PVRL2.
GenevisibleiP32507. MM.

Interactioni

Subunit structurei

Can form trans-heterodimers with NECTIN3. Interacts with CD226. Binds with low affinity to TIGIT (By similarity). Receptor for alphaherpesvirus (such as murine HSV) entry into cells.By similarity2 Publications

GO - Molecular functioni

  • cell adhesion molecule binding Source: BHF-UCL
  • identical protein binding Source: MGI
  • protein homodimerization activity Source: HGNC
  • receptor binding Source: GO_Central

Protein-protein interaction databases

DIPiDIP-59964N.
IntActiP32507. 3 interactions.
STRINGi10090.ENSMUSP00000074898.

Structurei

Secondary structure

1
530
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi35 – 373Combined sources
Beta strandi40 – 456Combined sources
Beta strandi50 – 523Combined sources
Beta strandi54 – 563Combined sources
Beta strandi63 – 708Combined sources
Beta strandi76 – 816Combined sources
Turni82 – 843Combined sources
Beta strandi85 – 873Combined sources
Turni95 – 973Combined sources
Beta strandi98 – 1003Combined sources
Beta strandi104 – 1063Combined sources
Helixi108 – 1114Combined sources
Beta strandi116 – 1183Combined sources
Helixi123 – 1253Combined sources
Beta strandi127 – 13610Combined sources
Beta strandi139 – 15113Combined sources
Beta strandi154 – 1596Combined sources
Beta strandi165 – 1673Combined sources
Beta strandi169 – 18113Combined sources
Beta strandi184 – 1885Combined sources
Beta strandi194 – 2018Combined sources
Beta strandi208 – 2169Combined sources
Helixi220 – 2223Combined sources
Beta strandi226 – 2327Combined sources
Beta strandi236 – 2383Combined sources
Beta strandi240 – 2456Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4FMKX-ray2.56A32-250[»]
4FN0X-ray3.35A/B/C32-250[»]
4FS0X-ray3.25A32-250[»]
ProteinModelPortaliP32507.
SMRiP32507. Positions 33-341.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 147116Ig-like V-typeAdd
BLAST
Domaini153 – 24795Ig-like C2-type 1Add
BLAST
Domaini252 – 33786Ig-like C2-type 2Add
BLAST

Sequence similaritiesi

Belongs to the nectin family.Curated

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIB8. Eukaryota.
ENOG4111FZM. LUCA.
GeneTreeiENSGT00770000120512.
HOGENOMiHOG000237277.
HOVERGENiHBG019169.
InParanoidiP32507.
KOiK06531.
OMAiEDDMESQ.
OrthoDBiEOG7D59N6.
PhylomeDBiP32507.
TreeFamiTF331051.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR033318. Nectin-2.
[Graphical view]
PANTHERiPTHR23277:SF63. PTHR23277:SF63. 1 hit.
PfamiPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 3 hits.
SM00406. IGv. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 3 hits.
PROSITEiPS50835. IG_LIKE. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Beta (identifier: P32507-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARAAVLPPS RLSPTLPLLP LLLLLLQETG AQDVRVRVLP EVRGRLGGTV
60 70 80 90 100
ELPCHLLPPT TERVSQVTWQ RLDGTVVAAF HPSFGVDFPN SQFSKDRLSF
110 120 130 140 150
VRARPETNAD LRDATLAFRG LRVEDEGNYT CEFATFPNGT RRGVTWLRVI
160 170 180 190 200
AQPENHAEAQ EVTIGPQSVA VARCVSTGGR PPARITWISS LGGEAKDTQE
210 220 230 240 250
PGIQAGTVTI ISRYSLVPVG RADGVKVTCR VEHESFEEPI LLPVTLSVRY
260 270 280 290 300
PPEVSISGYD DNWYLGRSEA ILTCDVRSNP EPTDYDWSTT SGVFPASAVA
310 320 330 340 350
QGSQLLVHSV DRMVNTTFIC TATNAVGTGR AEQVILVRES PSTAGAGATG
360 370 380 390 400
GIIGGIIAAI IATAVAGTGI LICRQQRKEQ RLQAADEEEE LEGPPSYKPP
410 420 430 440 450
TPKAKLEEPE MPSQLFTLGA SEHSPVKTPY FDAGVSCADQ EMPRYHELPT
460 470 480 490 500
LEERSGPLLL GATGLGPSLL VPPGPNVVEG VSLSLEDEEE DDEEEDFLDK
510 520 530
INPIYDALSY PSPSDSYQSK DFFVSRAMYV
Length:530
Mass (Da):57,318
Last modified:January 11, 2001 - v2
Checksum:i0ED71BFA2B231BBE
GO
Isoform Alpha (identifier: P32507-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     339-467: ESPSTAGAGA...LLLGATGLGP → DTPQASRDVG...SLISRRAVYV
     468-530: Missing.

Show »
Length:467
Mass (Da):50,779
Checksum:i7DB2430F6FFDD333
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei339 – 467129ESPST…TGLGP → DTPQASRDVGPLVWGAVGGT LLVLLLAGGFLALILLRGRR RRKSPGGGGNDGDRGSYDPK TQVFGNGGPVFWRSASPEPM RPDGREEDEEEEEEMKAEEG LMLPPHESPKDDMESHLDGS LISRRAVYV in isoform Alpha. 1 PublicationVSP_002630Add
BLAST
Alternative sequencei468 – 53063Missing in isoform Alpha. 1 PublicationVSP_002631Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80206 mRNA. Translation: AAA39734.1.
D26107 mRNA. Translation: BAA05103.1.
BC059941 mRNA. Translation: AAH59941.1.
CCDSiCCDS20913.1. [P32507-1]
CCDS52063.1. [P32507-2]
PIRiA38211. HLMSP3.
A53437.
RefSeqiNP_001153196.1. NM_001159724.1. [P32507-2]
NP_033016.3. NM_008990.3. [P32507-1]
UniGeneiMm.4341.

Genome annotation databases

EnsembliENSMUST00000075447; ENSMUSP00000074898; ENSMUSG00000062300. [P32507-1]
ENSMUST00000108450; ENSMUSP00000104089; ENSMUSG00000062300. [P32507-2]
GeneIDi19294.
KEGGimmu:19294.
UCSCiuc009fnd.2. mouse. [P32507-1]
uc009fne.3. mouse. [P32507-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80206 mRNA. Translation: AAA39734.1.
D26107 mRNA. Translation: BAA05103.1.
BC059941 mRNA. Translation: AAH59941.1.
CCDSiCCDS20913.1. [P32507-1]
CCDS52063.1. [P32507-2]
PIRiA38211. HLMSP3.
A53437.
RefSeqiNP_001153196.1. NM_001159724.1. [P32507-2]
NP_033016.3. NM_008990.3. [P32507-1]
UniGeneiMm.4341.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4FMKX-ray2.56A32-250[»]
4FN0X-ray3.35A/B/C32-250[»]
4FS0X-ray3.25A32-250[»]
ProteinModelPortaliP32507.
SMRiP32507. Positions 33-341.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59964N.
IntActiP32507. 3 interactions.
STRINGi10090.ENSMUSP00000074898.

PTM databases

iPTMnetiP32507.
PhosphoSiteiP32507.
SwissPalmiP32507.

Proteomic databases

MaxQBiP32507.
PaxDbiP32507.
PeptideAtlasiP32507.
PRIDEiP32507.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000075447; ENSMUSP00000074898; ENSMUSG00000062300. [P32507-1]
ENSMUST00000108450; ENSMUSP00000104089; ENSMUSG00000062300. [P32507-2]
GeneIDi19294.
KEGGimmu:19294.
UCSCiuc009fnd.2. mouse. [P32507-1]
uc009fne.3. mouse. [P32507-2]

Organism-specific databases

CTDi5819.
MGIiMGI:97822. Nectin2.

Phylogenomic databases

eggNOGiENOG410IIB8. Eukaryota.
ENOG4111FZM. LUCA.
GeneTreeiENSGT00770000120512.
HOGENOMiHOG000237277.
HOVERGENiHBG019169.
InParanoidiP32507.
KOiK06531.
OMAiEDDMESQ.
OrthoDBiEOG7D59N6.
PhylomeDBiP32507.
TreeFamiTF331051.

Enzyme and pathway databases

ReactomeiR-MMU-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-MMU-418990. Adherens junctions interactions.
R-MMU-420597. Nectin/Necl trans heterodimerization.

Miscellaneous databases

ChiTaRSiPvrl2. mouse.
PROiP32507.
SOURCEiSearch...

Gene expression databases

BgeeiP32507.
CleanExiMM_PVRL2.
GenevisibleiP32507. MM.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR033318. Nectin-2.
[Graphical view]
PANTHERiPTHR23277:SF63. PTHR23277:SF63. 1 hit.
PfamiPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 3 hits.
SM00406. IGv. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 3 hits.
PROSITEiPS50835. IG_LIKE. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression of a murine homolog of the human poliovirus receptor gene."
    Morrison M.E., Racaniello V.R.
    J. Virol. 66:2807-2813(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA).
  2. "Amino acid residues on human poliovirus receptor involved in interaction with poliovirus."
    Aoki J., Koike S., Ise I., Sato-Yoshida Y., Nomoto A.
    J. Biol. Chem. 269:8431-8438(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA).
    Strain: C57BL/6J.
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BETA).
    Strain: FVB/N.
    Tissue: Colon.
  4. "The murine homolog (Mph) of human herpesvirus entry protein B (HveB) mediates entry of pseudorabies virus but not herpes simplex virus types 1 and 2."
    Shukla D., Rowe C.L., Dong Y., Racaniello V.R., Spear P.G.
    J. Virol. 73:4493-4497(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "Nectin-3: a new member of immunoglobulin-like cell adhesion molecules that shows homophilic and heterophilic cell-cell adhesion activities."
    Satoh-Horikawa K., Nakanishi H., Takahashi K., Miyahara M., Nishimura M., Tachibana K., Mizoguchi A., Takai Y.
    J. Biol. Chem. 275:10291-10299(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NECTIN3.
  6. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-128; ASN-138 AND ASN-315.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-401 AND SER-424, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Heart, Kidney, Liver, Lung and Testis.
  8. Cited for: X-RAY CRYSTALLOGRAPHY (2.56 ANGSTROMS) OF 32-250, SUBUNIT, DISULFIDE BONDS, GLYCOSYLATION AT ASN-128 AND ASN-138, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiNECT2_MOUSE
AccessioniPrimary (citable) accession number: P32507
Secondary accession number(s): Q62096
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 11, 2001
Last modified: July 6, 2016
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.