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Protein

Nectin-2

Gene

Nectin2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive. Probable cell adhesion protein.By similarity

GO - Molecular functioni

  • cell adhesion molecule binding Source: BHF-UCL
  • protein homodimerization activity Source: HGNC
  • receptor activity Source: GO_Central
  • receptor binding Source: GO_Central

GO - Biological processi

  • acrosome assembly Source: MGI
  • adhesion of symbiont to host Source: MGI
  • cell part morphogenesis Source: MGI
  • cell recognition Source: GO_Central
  • cilium organization Source: MGI
  • coreceptor-mediated virion attachment to host cell Source: MGI
  • cytoskeleton organization Source: MGI
  • establishment of mitochondrion localization Source: MGI
  • fertilization Source: MGI
  • fusion of virus membrane with host plasma membrane Source: MGI
  • heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: GO_Central
  • homophilic cell adhesion via plasma membrane adhesion molecules Source: HGNC
  • positive regulation of immunoglobulin mediated immune response Source: MGI
  • positive regulation of mast cell activation Source: MGI
  • positive regulation of natural killer cell mediated cytotoxicity Source: MGI
  • positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target Source: MGI
  • regulation of viral entry into host cell Source: MGI
  • spermatid development Source: BHF-UCL
  • spermatid nucleus differentiation Source: MGI
  • sperm mitochondrion organization Source: MGI
  • susceptibility to natural killer cell mediated cytotoxicity Source: MGI
  • susceptibility to T cell mediated cytotoxicity Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-MMU-418990. Adherens junctions interactions.
R-MMU-420597. Nectin/Necl trans heterodimerization.

Names & Taxonomyi

Protein namesi
Recommended name:
Nectin-2
Alternative name(s):
Herpes virus entry mediator B
Short name:
Herpesvirus entry mediator B
Short name:
HveB
Murine herpes virus entry protein B
Short name:
mHveB
Nectin cell adhesion molecule 2By similarity
Poliovirus receptor homolog
Poliovirus receptor-related protein 2
CD_antigen: CD112
Gene namesi
Name:Nectin2By similarity
Synonyms:Mph, Pvr, Pvrl2, Pvs
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:97822. Nectin2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini32 – 351ExtracellularSequence analysisAdd BLAST320
Transmembranei352 – 372HelicalSequence analysisAdd BLAST21
Topological domaini373 – 530CytoplasmicSequence analysisAdd BLAST158

GO - Cellular componenti

  • apical junction complex Source: MGI
  • cell-cell contact zone Source: MGI
  • cell-cell junction Source: MGI
  • cell surface Source: MGI
  • extracellular exosome Source: MGI
  • focal adhesion Source: MGI
  • integral component of membrane Source: MGI
  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: HGNC
  • zonula adherens Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_000001513732 – 530Nectin-2Add BLAST499

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi54 ↔ 131PROSITE-ProRule annotation1 Publication
Glycosylationi128N-linked (GlcNAc...)2 Publications1
Glycosylationi138N-linked (GlcNAc...)2 Publications1
Disulfide bondi174 ↔ 229PROSITE-ProRule annotation1 Publication
Disulfide bondi274 ↔ 320PROSITE-ProRule annotation
Glycosylationi315N-linked (GlcNAc...)1 Publication1
Modified residuei401PhosphothreonineCombined sources1
Modified residuei424PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP32507.
PaxDbiP32507.
PeptideAtlasiP32507.
PRIDEiP32507.

PTM databases

iPTMnetiP32507.
PhosphoSitePlusiP32507.
SwissPalmiP32507.

Expressioni

Tissue specificityi

Brain, spinal cord, spleen, kidney, heart and liver.

Gene expression databases

BgeeiENSMUSG00000062300.
CleanExiMM_PVRL2.
GenevisibleiP32507. MM.

Interactioni

Subunit structurei

Can form trans-heterodimers with NECTIN3 (PubMed:10744716, PubMed:22902367). Interacts with CD226 or with PVRIG; these interactions are competitive and have a differential functional outcome on T-cell activation, either positive or negative, respectively. Binds with low affinity to TIGIT (By similarity).By similarity2 Publications

GO - Molecular functioni

  • cell adhesion molecule binding Source: BHF-UCL
  • protein homodimerization activity Source: HGNC
  • receptor binding Source: GO_Central

Protein-protein interaction databases

DIPiDIP-59964N.
IntActiP32507. 3 interactors.
STRINGi10090.ENSMUSP00000074898.

Structurei

Secondary structure

1530
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi35 – 37Combined sources3
Beta strandi40 – 45Combined sources6
Beta strandi50 – 52Combined sources3
Beta strandi54 – 56Combined sources3
Beta strandi63 – 70Combined sources8
Beta strandi76 – 81Combined sources6
Turni82 – 84Combined sources3
Beta strandi85 – 87Combined sources3
Turni95 – 97Combined sources3
Beta strandi98 – 100Combined sources3
Beta strandi104 – 106Combined sources3
Helixi108 – 111Combined sources4
Beta strandi116 – 118Combined sources3
Helixi123 – 125Combined sources3
Beta strandi127 – 136Combined sources10
Beta strandi139 – 151Combined sources13
Beta strandi154 – 159Combined sources6
Beta strandi165 – 167Combined sources3
Beta strandi169 – 181Combined sources13
Beta strandi184 – 188Combined sources5
Beta strandi194 – 201Combined sources8
Beta strandi208 – 216Combined sources9
Helixi220 – 222Combined sources3
Beta strandi226 – 232Combined sources7
Beta strandi236 – 238Combined sources3
Beta strandi240 – 245Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4FMKX-ray2.56A32-250[»]
4FN0X-ray3.35A/B/C32-250[»]
4FS0X-ray3.25A32-250[»]
ProteinModelPortaliP32507.
SMRiP32507.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 147Ig-like V-typeAdd BLAST116
Domaini153 – 247Ig-like C2-type 1Add BLAST95
Domaini252 – 337Ig-like C2-type 2Add BLAST86

Sequence similaritiesi

Belongs to the nectin family.Curated

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIB8. Eukaryota.
ENOG4111FZM. LUCA.
GeneTreeiENSGT00770000120512.
HOGENOMiHOG000237277.
HOVERGENiHBG019169.
InParanoidiP32507.
KOiK06531.
OMAiREVTWLR.
OrthoDBiEOG091G0CPW.
PhylomeDBiP32507.
TreeFamiTF331051.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR033318. Nectin-2.
[Graphical view]
PANTHERiPTHR23277:SF63. PTHR23277:SF63. 1 hit.
PfamiPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 3 hits.
SM00406. IGv. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 3 hits.
PROSITEiPS50835. IG_LIKE. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Beta (identifier: P32507-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARAAVLPPS RLSPTLPLLP LLLLLLQETG AQDVRVRVLP EVRGRLGGTV
60 70 80 90 100
ELPCHLLPPT TERVSQVTWQ RLDGTVVAAF HPSFGVDFPN SQFSKDRLSF
110 120 130 140 150
VRARPETNAD LRDATLAFRG LRVEDEGNYT CEFATFPNGT RRGVTWLRVI
160 170 180 190 200
AQPENHAEAQ EVTIGPQSVA VARCVSTGGR PPARITWISS LGGEAKDTQE
210 220 230 240 250
PGIQAGTVTI ISRYSLVPVG RADGVKVTCR VEHESFEEPI LLPVTLSVRY
260 270 280 290 300
PPEVSISGYD DNWYLGRSEA ILTCDVRSNP EPTDYDWSTT SGVFPASAVA
310 320 330 340 350
QGSQLLVHSV DRMVNTTFIC TATNAVGTGR AEQVILVRES PSTAGAGATG
360 370 380 390 400
GIIGGIIAAI IATAVAGTGI LICRQQRKEQ RLQAADEEEE LEGPPSYKPP
410 420 430 440 450
TPKAKLEEPE MPSQLFTLGA SEHSPVKTPY FDAGVSCADQ EMPRYHELPT
460 470 480 490 500
LEERSGPLLL GATGLGPSLL VPPGPNVVEG VSLSLEDEEE DDEEEDFLDK
510 520 530
INPIYDALSY PSPSDSYQSK DFFVSRAMYV
Length:530
Mass (Da):57,318
Last modified:January 11, 2001 - v2
Checksum:i0ED71BFA2B231BBE
GO
Isoform Alpha (identifier: P32507-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     339-467: ESPSTAGAGA...LLLGATGLGP → DTPQASRDVG...SLISRRAVYV
     468-530: Missing.

Show »
Length:467
Mass (Da):50,779
Checksum:i7DB2430F6FFDD333
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002630339 – 467ESPST…TGLGP → DTPQASRDVGPLVWGAVGGT LLVLLLAGGFLALILLRGRR RRKSPGGGGNDGDRGSYDPK TQVFGNGGPVFWRSASPEPM RPDGREEDEEEEEEMKAEEG LMLPPHESPKDDMESHLDGS LISRRAVYV in isoform Alpha. 1 PublicationAdd BLAST129
Alternative sequenceiVSP_002631468 – 530Missing in isoform Alpha. 1 PublicationAdd BLAST63

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80206 mRNA. Translation: AAA39734.1.
D26107 mRNA. Translation: BAA05103.1.
BC059941 mRNA. Translation: AAH59941.1.
CCDSiCCDS20913.1. [P32507-1]
CCDS52063.1. [P32507-2]
PIRiA38211. HLMSP3.
A53437.
RefSeqiNP_001153196.1. NM_001159724.1. [P32507-2]
NP_033016.3. NM_008990.3. [P32507-1]
UniGeneiMm.4341.

Genome annotation databases

EnsembliENSMUST00000075447; ENSMUSP00000074898; ENSMUSG00000062300. [P32507-1]
ENSMUST00000108450; ENSMUSP00000104089; ENSMUSG00000062300. [P32507-2]
GeneIDi19294.
KEGGimmu:19294.
UCSCiuc009fnd.2. mouse. [P32507-1]
uc009fne.3. mouse. [P32507-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80206 mRNA. Translation: AAA39734.1.
D26107 mRNA. Translation: BAA05103.1.
BC059941 mRNA. Translation: AAH59941.1.
CCDSiCCDS20913.1. [P32507-1]
CCDS52063.1. [P32507-2]
PIRiA38211. HLMSP3.
A53437.
RefSeqiNP_001153196.1. NM_001159724.1. [P32507-2]
NP_033016.3. NM_008990.3. [P32507-1]
UniGeneiMm.4341.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4FMKX-ray2.56A32-250[»]
4FN0X-ray3.35A/B/C32-250[»]
4FS0X-ray3.25A32-250[»]
ProteinModelPortaliP32507.
SMRiP32507.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59964N.
IntActiP32507. 3 interactors.
STRINGi10090.ENSMUSP00000074898.

PTM databases

iPTMnetiP32507.
PhosphoSitePlusiP32507.
SwissPalmiP32507.

Proteomic databases

MaxQBiP32507.
PaxDbiP32507.
PeptideAtlasiP32507.
PRIDEiP32507.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000075447; ENSMUSP00000074898; ENSMUSG00000062300. [P32507-1]
ENSMUST00000108450; ENSMUSP00000104089; ENSMUSG00000062300. [P32507-2]
GeneIDi19294.
KEGGimmu:19294.
UCSCiuc009fnd.2. mouse. [P32507-1]
uc009fne.3. mouse. [P32507-2]

Organism-specific databases

CTDi5819.
MGIiMGI:97822. Nectin2.

Phylogenomic databases

eggNOGiENOG410IIB8. Eukaryota.
ENOG4111FZM. LUCA.
GeneTreeiENSGT00770000120512.
HOGENOMiHOG000237277.
HOVERGENiHBG019169.
InParanoidiP32507.
KOiK06531.
OMAiREVTWLR.
OrthoDBiEOG091G0CPW.
PhylomeDBiP32507.
TreeFamiTF331051.

Enzyme and pathway databases

ReactomeiR-MMU-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-MMU-418990. Adherens junctions interactions.
R-MMU-420597. Nectin/Necl trans heterodimerization.

Miscellaneous databases

ChiTaRSiPvrl2. mouse.
PROiP32507.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000062300.
CleanExiMM_PVRL2.
GenevisibleiP32507. MM.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR033318. Nectin-2.
[Graphical view]
PANTHERiPTHR23277:SF63. PTHR23277:SF63. 1 hit.
PfamiPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 3 hits.
SM00406. IGv. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 3 hits.
PROSITEiPS50835. IG_LIKE. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNECT2_MOUSE
AccessioniPrimary (citable) accession number: P32507
Secondary accession number(s): Q62096
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 11, 2001
Last modified: November 30, 2016
This is version 155 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.