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Protein

Nectin-2

Gene

Nectin2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive. Probable cell adhesion protein.By similarity

GO - Molecular functioni

  • cell adhesion molecule binding Source: BHF-UCL
  • identical protein binding Source: IntAct
  • protein homodimerization activity Source: HGNC
  • signaling receptor binding Source: GO_Central

GO - Biological processi

  • acrosome assembly Source: MGI
  • adhesion of symbiont to host Source: MGI
  • cell part morphogenesis Source: MGI
  • cell recognition Source: GO_Central
  • cilium organization Source: MGI
  • coreceptor-mediated virion attachment to host cell Source: MGI
  • cytoskeleton organization Source: MGI
  • establishment of mitochondrion localization Source: MGI
  • fertilization Source: MGI
  • fusion of virus membrane with host plasma membrane Source: MGI
  • heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: GO_Central
  • homophilic cell adhesion via plasma membrane adhesion molecules Source: HGNC
  • positive regulation of immunoglobulin mediated immune response Source: MGI
  • positive regulation of mast cell activation Source: MGI
  • positive regulation of natural killer cell mediated cytotoxicity Source: MGI
  • positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target Source: MGI
  • positive regulation of T cell receptor signaling pathway Source: MGI
  • regulation of viral entry into host cell Source: MGI
  • spermatid development Source: BHF-UCL
  • spermatid nucleus differentiation Source: MGI
  • sperm mitochondrion organization Source: MGI
  • susceptibility to natural killer cell mediated cytotoxicity Source: MGI
  • susceptibility to T cell mediated cytotoxicity Source: MGI

Keywordsi

Molecular functionReceptor
Biological processCell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
R-MMU-418990 Adherens junctions interactions
R-MMU-420597 Nectin/Necl trans heterodimerization

Names & Taxonomyi

Protein namesi
Recommended name:
Nectin-2
Alternative name(s):
Herpes virus entry mediator B
Short name:
Herpesvirus entry mediator B
Short name:
HveB
Murine herpes virus entry protein B
Short name:
mHveB
Nectin cell adhesion molecule 2By similarity
Poliovirus receptor homolog
Poliovirus receptor-related protein 2
CD_antigen: CD112
Gene namesi
Name:Nectin2By similarity
Synonyms:Mph, Pvr, Pvrl2, Pvs
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:97822 Nectin2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini32 – 351ExtracellularSequence analysisAdd BLAST320
Transmembranei352 – 372HelicalSequence analysisAdd BLAST21
Topological domaini373 – 530CytoplasmicSequence analysisAdd BLAST158

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_000001513732 – 530Nectin-2Add BLAST499

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi54 ↔ 131PROSITE-ProRule annotation1 Publication
Glycosylationi128N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi138N-linked (GlcNAc...) asparagine2 Publications1
Disulfide bondi174 ↔ 229PROSITE-ProRule annotation1 Publication
Disulfide bondi274 ↔ 320PROSITE-ProRule annotation
Glycosylationi315N-linked (GlcNAc...) asparagine1 Publication1
Modified residuei401PhosphothreonineCombined sources1
Modified residuei424PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP32507
PaxDbiP32507
PeptideAtlasiP32507
PRIDEiP32507

PTM databases

iPTMnetiP32507
PhosphoSitePlusiP32507
SwissPalmiP32507

Expressioni

Tissue specificityi

Brain, spinal cord, spleen, kidney, heart and liver.

Gene expression databases

BgeeiENSMUSG00000062300
CleanExiMM_PVRL2
GenevisibleiP32507 MM

Interactioni

Subunit structurei

Can form trans-heterodimers with NECTIN3 (PubMed:10744716, PubMed:22902367). Interacts with CD226 or with PVRIG; these interactions are competitive and have a differential functional outcome on T-cell activation, either positive or negative, respectively. Binds with low affinity to TIGIT (By similarity).By similarity2 Publications

GO - Molecular functioni

  • cell adhesion molecule binding Source: BHF-UCL
  • identical protein binding Source: IntAct
  • protein homodimerization activity Source: HGNC
  • signaling receptor binding Source: GO_Central

Protein-protein interaction databases

BioGridi202518, 1 interactor
DIPiDIP-59964N
IntActiP32507, 3 interactors
STRINGi10090.ENSMUSP00000074898

Structurei

Secondary structure

1530
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi35 – 37Combined sources3
Beta strandi40 – 45Combined sources6
Beta strandi50 – 52Combined sources3
Beta strandi54 – 56Combined sources3
Beta strandi63 – 70Combined sources8
Beta strandi76 – 81Combined sources6
Turni82 – 84Combined sources3
Beta strandi85 – 87Combined sources3
Turni95 – 97Combined sources3
Beta strandi98 – 100Combined sources3
Beta strandi104 – 106Combined sources3
Helixi108 – 111Combined sources4
Beta strandi116 – 118Combined sources3
Helixi123 – 125Combined sources3
Beta strandi127 – 136Combined sources10
Beta strandi139 – 151Combined sources13
Beta strandi154 – 159Combined sources6
Beta strandi165 – 167Combined sources3
Beta strandi169 – 181Combined sources13
Beta strandi184 – 188Combined sources5
Beta strandi194 – 201Combined sources8
Beta strandi208 – 216Combined sources9
Helixi220 – 222Combined sources3
Beta strandi226 – 232Combined sources7
Beta strandi236 – 238Combined sources3
Beta strandi240 – 245Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4FMKX-ray2.56A32-250[»]
4FN0X-ray3.35A/B/C32-250[»]
4FS0X-ray3.25A32-250[»]
ProteinModelPortaliP32507
SMRiP32507
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 147Ig-like V-typeAdd BLAST116
Domaini153 – 247Ig-like C2-type 1Add BLAST95
Domaini252 – 337Ig-like C2-type 2Add BLAST86

Sequence similaritiesi

Belongs to the nectin family.Curated

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIB8 Eukaryota
ENOG4111FZM LUCA
GeneTreeiENSGT00900000140811
HOGENOMiHOG000237277
HOVERGENiHBG019169
InParanoidiP32507
KOiK06531
OMAiTVNLRCA
OrthoDBiEOG091G0CPW
PhylomeDBiP32507
TreeFamiTF331051

Family and domain databases

Gene3Di2.60.40.10, 3 hits
InterProiView protein in InterPro
IPR013162 CD80_C2-set
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003599 Ig_sub
IPR013106 Ig_V-set
IPR033318 Nectin-2
PANTHERiPTHR23277:SF63 PTHR23277:SF63, 1 hit
PfamiView protein in Pfam
PF08205 C2-set_2, 1 hit
PF07686 V-set, 1 hit
SMARTiView protein in SMART
SM00409 IG, 3 hits
SM00406 IGv, 1 hit
SUPFAMiSSF48726 SSF48726, 3 hits
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 3 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Beta (identifier: P32507-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARAAVLPPS RLSPTLPLLP LLLLLLQETG AQDVRVRVLP EVRGRLGGTV
60 70 80 90 100
ELPCHLLPPT TERVSQVTWQ RLDGTVVAAF HPSFGVDFPN SQFSKDRLSF
110 120 130 140 150
VRARPETNAD LRDATLAFRG LRVEDEGNYT CEFATFPNGT RRGVTWLRVI
160 170 180 190 200
AQPENHAEAQ EVTIGPQSVA VARCVSTGGR PPARITWISS LGGEAKDTQE
210 220 230 240 250
PGIQAGTVTI ISRYSLVPVG RADGVKVTCR VEHESFEEPI LLPVTLSVRY
260 270 280 290 300
PPEVSISGYD DNWYLGRSEA ILTCDVRSNP EPTDYDWSTT SGVFPASAVA
310 320 330 340 350
QGSQLLVHSV DRMVNTTFIC TATNAVGTGR AEQVILVRES PSTAGAGATG
360 370 380 390 400
GIIGGIIAAI IATAVAGTGI LICRQQRKEQ RLQAADEEEE LEGPPSYKPP
410 420 430 440 450
TPKAKLEEPE MPSQLFTLGA SEHSPVKTPY FDAGVSCADQ EMPRYHELPT
460 470 480 490 500
LEERSGPLLL GATGLGPSLL VPPGPNVVEG VSLSLEDEEE DDEEEDFLDK
510 520 530
INPIYDALSY PSPSDSYQSK DFFVSRAMYV
Length:530
Mass (Da):57,318
Last modified:January 11, 2001 - v2
Checksum:i0ED71BFA2B231BBE
GO
Isoform Alpha (identifier: P32507-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     339-467: ESPSTAGAGA...LLLGATGLGP → DTPQASRDVG...SLISRRAVYV
     468-530: Missing.

Show »
Length:467
Mass (Da):50,779
Checksum:i7DB2430F6FFDD333
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002630339 – 467ESPST…TGLGP → DTPQASRDVGPLVWGAVGGT LLVLLLAGGFLALILLRGRR RRKSPGGGGNDGDRGSYDPK TQVFGNGGPVFWRSASPEPM RPDGREEDEEEEEEMKAEEG LMLPPHESPKDDMESHLDGS LISRRAVYV in isoform Alpha. 1 PublicationAdd BLAST129
Alternative sequenceiVSP_002631468 – 530Missing in isoform Alpha. 1 PublicationAdd BLAST63

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80206 mRNA Translation: AAA39734.1
D26107 mRNA Translation: BAA05103.1
BC059941 mRNA Translation: AAH59941.1
CCDSiCCDS20913.1 [P32507-1]
CCDS52063.1 [P32507-2]
PIRiA38211 HLMSP3
A53437
RefSeqiNP_001153196.1, NM_001159724.1 [P32507-2]
NP_033016.3, NM_008990.3 [P32507-1]
UniGeneiMm.4341

Genome annotation databases

EnsembliENSMUST00000075447; ENSMUSP00000074898; ENSMUSG00000062300 [P32507-1]
ENSMUST00000108450; ENSMUSP00000104089; ENSMUSG00000062300 [P32507-2]
GeneIDi19294
KEGGimmu:19294
UCSCiuc009fnd.2 mouse [P32507-1]
uc009fne.3 mouse [P32507-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNECT2_MOUSE
AccessioniPrimary (citable) accession number: P32507
Secondary accession number(s): Q62096
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 11, 2001
Last modified: May 23, 2018
This is version 165 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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