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P32505 (NAB2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear polyadenylated RNA-binding protein NAB2
Gene names
Name:NAB2
Ordered Locus Names:YGL122C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length525 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This essential protein binds to polyadenylated RNA and single-stranded DNA. It may be involved not only in RNA processing but also in transcription regulation. Believed to associate directly with nascent RNA polymerase II transcripts and remain associated during subsequent nuclear RNA processing reactions.

Subcellular location

Nucleus.

Miscellaneous

Present with 9670 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the NAB2 family.

Contains 7 C3H1-type zinc fingers.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 525525Nuclear polyadenylated RNA-binding protein NAB2
PRO_0000096687

Regions

Repeat121 – 12441
Repeat125 – 12842
Repeat129 – 13243
Repeat133 – 13644
Repeat137 – 14045
Repeat141 – 14446
Repeat145 – 14847
Repeat149 – 15248
Repeat153 – 15649
Repeat157 – 160410; approximate
Zinc finger262 – 27817C3H1-type 1
Zinc finger283 – 30018C3H1-type 2
Zinc finger340 – 35516C3H1-type 3
Zinc finger371 – 38616C3H1-type 4
Zinc finger415 – 43016C3H1-type 5
Zinc finger437 – 45216C3H1-type 6
Zinc finger458 – 47316C3H1-type 7
Region121 – 1563610 X 4 AA tandem repeats of Q-Q-Q-P
Region209 – 22820RNA-binding RGG-box By similarity
Compositional bias97 – 17276Gln-rich
Compositional bias106 – 11611Poly-Gln

Natural variations

Natural variant130 – 15728Missing in YJA512.

Secondary structure

............................................. 525
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P32505 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 40335C3D4658DD91

FASTA52558,321
        10         20         30         40         50         60 
MSQEQYTENL KVIVAEKLAG IPNFNEDIKY VAEYIVLLIV NGGTVESVVD ELASLFDSVS 

        70         80         90        100        110        120 
RDTLANVVQT AFFALEALQQ GESAENIVSK IRMMNAQSLG QSDIAQQQQQ QQQQQQPDIA 

       130        140        150        160        170        180 
QQQPQQQPQQ QPQQQPQQQP QQQPQQQPQQ QPQQQPQLQP LQPQLGTQNA MQTDAPATPS 

       190        200        210        220        230        240 
PISAFSGVVN AAAPPQFAPV DNSQRFTQRG GGAVGKNRRG GRGGNRGGRN NNSTRFNPLA 

       250        260        270        280        290        300 
KALGMAGESN MNFTPTKKEG RCRLFPHCPL GRSCPHAHPT KVCNEYPNCP KPPGTCEFLH 

       310        320        330        340        350        360 
PNEDEELMKE MERTREEFQK RKADLLAAKR KPVQTGIVLC KFGALCSNPS CPFGHPTPAN 

       370        380        390        400        410        420 
EDAKVIDLMW CDKNLTCDNP ECRKAHSSLS KIKEVKPISQ KKAAPPPVEK SLEQCKFGTH 

       430        440        450        460        470        480 
CTNKRCKYRH ARSHIMCREG ANCTRIDCLF GHPINEDCRF GVNCKNIYCL FRHPPGRVLP 

       490        500        510        520 
EKKGAAPNSN VPTNERPFAL PENAIIENAP PQTSFTHQEQ DTEMN 

« Hide

References

« Hide 'large scale' references
[1]"NAB2: a yeast nuclear polyadenylated RNA-binding protein essential for cell viability."
Anderson J.T., Wilson S.M., Datar K.V., Swanson M.S.
Mol. Cell. Biol. 13:2730-2741(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 208279 / BJ926.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L10288 Genomic DNA. Translation: AAA34819.1.
L08079 Genomic DNA. Translation: AAA34820.1.
Z72644 Genomic DNA. Translation: CAA96830.1.
AY723810 Genomic DNA. Translation: AAU09727.1.
BK006941 Genomic DNA. Translation: DAA07987.1.
PIRB48058.
RefSeqNP_011393.3. NM_001180987.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2JPSNMR-A1-105[»]
2LHNNMR-A409-483[»]
2V75X-ray1.80A1-104[»]
3LCNX-ray2.00A/B1-105[»]
3ZJ1NMR-A253-333[»]
3ZJ2NMR-A333-401[»]
4JLQX-ray3.04B205-242[»]
ProteinModelPortalP32505.
SMRP32505. Positions 6-94, 252-483.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33129. 2589 interactions.
DIPDIP-1331N.
IntActP32505. 17 interactions.
MINTMINT-385635.
STRING4932.YGL122C.

Proteomic databases

PaxDbP32505.
PeptideAtlasP32505.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL122C; YGL122C; YGL122C.
GeneID852755.
KEGGsce:YGL122C.

Organism-specific databases

CYGDYGL122c.
SGDS000003090. NAB2.

Phylogenomic databases

eggNOGNOG330419.
HOGENOMHOG000246536.
OMAGANCTRI.
OrthoDBEOG7F7WKM.

Enzyme and pathway databases

BioCycYEAST:G3O-30619-MONOMER.

Gene expression databases

GenevestigatorP32505.

Family and domain databases

InterProIPR021083. Poly(A)_RNA-bd_protein_2_Nab2.
[Graphical view]
PfamPF11517. Nab2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP32505.
NextBio972195.

Entry information

Entry nameNAB2_YEAST
AccessionPrimary (citable) accession number: P32505
Secondary accession number(s): D6VU26
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: April 16, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references