SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P32505

- NAB2_YEAST

UniProt

P32505 - NAB2_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Nuclear polyadenylated RNA-binding protein NAB2
Gene
NAB2, YGL122C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

This essential protein binds to polyadenylated RNA and single-stranded DNA. It may be involved not only in RNA processing but also in transcription regulation. Believed to associate directly with nascent RNA polymerase II transcripts and remain associated during subsequent nuclear RNA processing reactions.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri262 – 27817C3H1-type 1
Add
BLAST
Zinc fingeri283 – 30018C3H1-type 2
Add
BLAST
Zinc fingeri340 – 35516C3H1-type 3
Add
BLAST
Zinc fingeri371 – 38616C3H1-type 4
Add
BLAST
Zinc fingeri415 – 43016C3H1-type 5
Add
BLAST
Zinc fingeri437 – 45216C3H1-type 6
Add
BLAST
Zinc fingeri458 – 47316C3H1-type 7
Add
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. poly(A) binding Source: SGD
  3. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. mRNA polyadenylation Source: SGD
  2. poly(A)+ mRNA export from nucleus Source: SGD
  3. regulation of mRNA stability Source: SGD
Complete GO annotation...

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30619-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear polyadenylated RNA-binding protein NAB2
Gene namesi
Name:NAB2
Ordered Locus Names:YGL122C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL122c.
SGDiS000003090. NAB2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 525525Nuclear polyadenylated RNA-binding protein NAB2
PRO_0000096687Add
BLAST

Proteomic databases

MaxQBiP32505.
PaxDbiP32505.
PeptideAtlasiP32505.

Expressioni

Gene expression databases

GenevestigatoriP32505.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
GFD1Q048397EBI-11770,EBI-27549
HMT1P380742EBI-11770,EBI-8394
HRP1Q993833EBI-11770,EBI-11783
MLP1Q024554EBI-11770,EBI-11009

Protein-protein interaction databases

BioGridi33129. 2589 interactions.
DIPiDIP-1331N.
IntActiP32505. 17 interactions.
MINTiMINT-385635.
STRINGi4932.YGL122C.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 1812
Helixi28 – 4013
Helixi45 – 5511
Helixi61 – 7919
Helixi84 – 9310
Turni269 – 2735
Turni293 – 2953
Beta strandi297 – 2993
Beta strandi301 – 3033
Helixi305 – 32622
Beta strandi328 – 3303
Helixi343 – 3453
Beta strandi353 – 3553
Helixi374 – 3763
Beta strandi384 – 3874
Helixi390 – 3934
Beta strandi412 – 4143
Helixi418 – 4203
Beta strandi427 – 4293
Helixi440 – 4423
Beta strandi455 – 4573
Beta strandi470 – 4723

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JPSNMR-A1-105[»]
2LHNNMR-A409-483[»]
2V75X-ray1.80A1-104[»]
3LCNX-ray2.00A/B1-105[»]
3ZJ1NMR-A253-333[»]
3ZJ2NMR-A333-401[»]
4JLQX-ray3.04B205-242[»]
ProteinModelPortaliP32505.
SMRiP32505. Positions 6-94, 252-401, 404-483.

Miscellaneous databases

EvolutionaryTraceiP32505.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati121 – 12441
Repeati125 – 12842
Repeati129 – 13243
Repeati133 – 13644
Repeati137 – 14045
Repeati141 – 14446
Repeati145 – 14847
Repeati149 – 15248
Repeati153 – 15649
Repeati157 – 160410; approximate

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni121 – 1563610 X 4 AA tandem repeats of Q-Q-Q-P
Add
BLAST
Regioni209 – 22820RNA-binding RGG-box By similarity
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi97 – 17276Gln-rich
Add
BLAST
Compositional biasi106 – 11611Poly-Gln
Add
BLAST

Sequence similaritiesi

Belongs to the NAB2 family.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG330419.
HOGENOMiHOG000246536.
OMAiGANCTRI.
OrthoDBiEOG7F7WKM.

Family and domain databases

InterProiIPR021083. Poly(A)_RNA-bd_protein_2_Nab2.
[Graphical view]
PfamiPF11517. Nab2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32505-1 [UniParc]FASTAAdd to Basket

« Hide

MSQEQYTENL KVIVAEKLAG IPNFNEDIKY VAEYIVLLIV NGGTVESVVD    50
ELASLFDSVS RDTLANVVQT AFFALEALQQ GESAENIVSK IRMMNAQSLG 100
QSDIAQQQQQ QQQQQQPDIA QQQPQQQPQQ QPQQQPQQQP QQQPQQQPQQ 150
QPQQQPQLQP LQPQLGTQNA MQTDAPATPS PISAFSGVVN AAAPPQFAPV 200
DNSQRFTQRG GGAVGKNRRG GRGGNRGGRN NNSTRFNPLA KALGMAGESN 250
MNFTPTKKEG RCRLFPHCPL GRSCPHAHPT KVCNEYPNCP KPPGTCEFLH 300
PNEDEELMKE MERTREEFQK RKADLLAAKR KPVQTGIVLC KFGALCSNPS 350
CPFGHPTPAN EDAKVIDLMW CDKNLTCDNP ECRKAHSSLS KIKEVKPISQ 400
KKAAPPPVEK SLEQCKFGTH CTNKRCKYRH ARSHIMCREG ANCTRIDCLF 450
GHPINEDCRF GVNCKNIYCL FRHPPGRVLP EKKGAAPNSN VPTNERPFAL 500
PENAIIENAP PQTSFTHQEQ DTEMN 525
Length:525
Mass (Da):58,321
Last modified:October 1, 1993 - v1
Checksum:i40335C3D4658DD91
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti130 – 15728Missing in YJA512.
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L10288 Genomic DNA. Translation: AAA34819.1.
L08079 Genomic DNA. Translation: AAA34820.1.
Z72644 Genomic DNA. Translation: CAA96830.1.
AY723810 Genomic DNA. Translation: AAU09727.1.
BK006941 Genomic DNA. Translation: DAA07987.1.
PIRiB48058.
RefSeqiNP_011393.3. NM_001180987.3.

Genome annotation databases

EnsemblFungiiYGL122C; YGL122C; YGL122C.
GeneIDi852755.
KEGGisce:YGL122C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L10288 Genomic DNA. Translation: AAA34819.1 .
L08079 Genomic DNA. Translation: AAA34820.1 .
Z72644 Genomic DNA. Translation: CAA96830.1 .
AY723810 Genomic DNA. Translation: AAU09727.1 .
BK006941 Genomic DNA. Translation: DAA07987.1 .
PIRi B48058.
RefSeqi NP_011393.3. NM_001180987.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2JPS NMR - A 1-105 [» ]
2LHN NMR - A 409-483 [» ]
2V75 X-ray 1.80 A 1-104 [» ]
3LCN X-ray 2.00 A/B 1-105 [» ]
3ZJ1 NMR - A 253-333 [» ]
3ZJ2 NMR - A 333-401 [» ]
4JLQ X-ray 3.04 B 205-242 [» ]
ProteinModelPortali P32505.
SMRi P32505. Positions 6-94, 252-401, 404-483.
ModBasei Search...

Protein-protein interaction databases

BioGridi 33129. 2589 interactions.
DIPi DIP-1331N.
IntActi P32505. 17 interactions.
MINTi MINT-385635.
STRINGi 4932.YGL122C.

Proteomic databases

MaxQBi P32505.
PaxDbi P32505.
PeptideAtlasi P32505.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL122C ; YGL122C ; YGL122C .
GeneIDi 852755.
KEGGi sce:YGL122C.

Organism-specific databases

CYGDi YGL122c.
SGDi S000003090. NAB2.

Phylogenomic databases

eggNOGi NOG330419.
HOGENOMi HOG000246536.
OMAi GANCTRI.
OrthoDBi EOG7F7WKM.

Enzyme and pathway databases

BioCyci YEAST:G3O-30619-MONOMER.

Miscellaneous databases

EvolutionaryTracei P32505.
NextBioi 972195.

Gene expression databases

Genevestigatori P32505.

Family and domain databases

InterProi IPR021083. Poly(A)_RNA-bd_protein_2_Nab2.
[Graphical view ]
Pfami PF11517. Nab2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "NAB2: a yeast nuclear polyadenylated RNA-binding protein essential for cell viability."
    Anderson J.T., Wilson S.M., Datar K.V., Swanson M.S.
    Mol. Cell. Biol. 13:2730-2741(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 208279 / BJ926.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNAB2_YEAST
AccessioniPrimary (citable) accession number: P32505
Secondary accession number(s): D6VU26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 11, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 9670 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi