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P32505

- NAB2_YEAST

UniProt

P32505 - NAB2_YEAST

Protein

Nuclear polyadenylated RNA-binding protein NAB2

Gene

NAB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    This essential protein binds to polyadenylated RNA and single-stranded DNA. It may be involved not only in RNA processing but also in transcription regulation. Believed to associate directly with nascent RNA polymerase II transcripts and remain associated during subsequent nuclear RNA processing reactions.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri262 – 27817C3H1-type 1Add
    BLAST
    Zinc fingeri283 – 30018C3H1-type 2Add
    BLAST
    Zinc fingeri340 – 35516C3H1-type 3Add
    BLAST
    Zinc fingeri371 – 38616C3H1-type 4Add
    BLAST
    Zinc fingeri415 – 43016C3H1-type 5Add
    BLAST
    Zinc fingeri437 – 45216C3H1-type 6Add
    BLAST
    Zinc fingeri458 – 47316C3H1-type 7Add
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. poly(A) binding Source: SGD
    3. protein binding Source: IntAct

    GO - Biological processi

    1. mRNA polyadenylation Source: SGD
    2. poly(A)+ mRNA export from nucleus Source: SGD
    3. regulation of mRNA stability Source: SGD

    Keywords - Ligandi

    Metal-binding, RNA-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30619-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear polyadenylated RNA-binding protein NAB2
    Gene namesi
    Name:NAB2
    Ordered Locus Names:YGL122C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL122c.
    SGDiS000003090. NAB2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 525525Nuclear polyadenylated RNA-binding protein NAB2PRO_0000096687Add
    BLAST

    Proteomic databases

    MaxQBiP32505.
    PaxDbiP32505.
    PeptideAtlasiP32505.

    Expressioni

    Gene expression databases

    GenevestigatoriP32505.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GFD1Q048397EBI-11770,EBI-27549
    HMT1P380742EBI-11770,EBI-8394
    HRP1Q993833EBI-11770,EBI-11783
    MLP1Q024554EBI-11770,EBI-11009

    Protein-protein interaction databases

    BioGridi33129. 2589 interactions.
    DIPiDIP-1331N.
    IntActiP32505. 17 interactions.
    MINTiMINT-385635.
    STRINGi4932.YGL122C.

    Structurei

    Secondary structure

    1
    525
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 1812
    Helixi28 – 4013
    Helixi45 – 5511
    Helixi61 – 7919
    Helixi84 – 9310
    Turni269 – 2735
    Turni293 – 2953
    Beta strandi297 – 2993
    Beta strandi301 – 3033
    Helixi305 – 32622
    Beta strandi328 – 3303
    Helixi343 – 3453
    Beta strandi353 – 3553
    Helixi374 – 3763
    Beta strandi384 – 3874
    Helixi390 – 3934
    Beta strandi412 – 4143
    Helixi418 – 4203
    Beta strandi427 – 4293
    Helixi440 – 4423
    Beta strandi455 – 4573
    Beta strandi470 – 4723

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2JPSNMR-A1-105[»]
    2LHNNMR-A409-483[»]
    2V75X-ray1.80A1-104[»]
    3LCNX-ray2.00A/B1-105[»]
    3ZJ1NMR-A253-333[»]
    3ZJ2NMR-A333-401[»]
    4JLQX-ray3.04B205-242[»]
    ProteinModelPortaliP32505.
    SMRiP32505. Positions 6-94, 252-401, 404-483.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP32505.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati121 – 12441
    Repeati125 – 12842
    Repeati129 – 13243
    Repeati133 – 13644
    Repeati137 – 14045
    Repeati141 – 14446
    Repeati145 – 14847
    Repeati149 – 15248
    Repeati153 – 15649
    Repeati157 – 160410; approximate

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni121 – 1563610 X 4 AA tandem repeats of Q-Q-Q-PAdd
    BLAST
    Regioni209 – 22820RNA-binding RGG-boxBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi97 – 17276Gln-richAdd
    BLAST
    Compositional biasi106 – 11611Poly-GlnAdd
    BLAST

    Sequence similaritiesi

    Belongs to the NAB2 family.Curated
    Contains 7 C3H1-type zinc fingers.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri262 – 27817C3H1-type 1Add
    BLAST
    Zinc fingeri283 – 30018C3H1-type 2Add
    BLAST
    Zinc fingeri340 – 35516C3H1-type 3Add
    BLAST
    Zinc fingeri371 – 38616C3H1-type 4Add
    BLAST
    Zinc fingeri415 – 43016C3H1-type 5Add
    BLAST
    Zinc fingeri437 – 45216C3H1-type 6Add
    BLAST
    Zinc fingeri458 – 47316C3H1-type 7Add
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG330419.
    HOGENOMiHOG000246536.
    OMAiGANCTRI.
    OrthoDBiEOG7F7WKM.

    Family and domain databases

    InterProiIPR021083. Poly(A)_RNA-bd_protein_2_Nab2.
    [Graphical view]
    PfamiPF11517. Nab2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32505-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQEQYTENL KVIVAEKLAG IPNFNEDIKY VAEYIVLLIV NGGTVESVVD    50
    ELASLFDSVS RDTLANVVQT AFFALEALQQ GESAENIVSK IRMMNAQSLG 100
    QSDIAQQQQQ QQQQQQPDIA QQQPQQQPQQ QPQQQPQQQP QQQPQQQPQQ 150
    QPQQQPQLQP LQPQLGTQNA MQTDAPATPS PISAFSGVVN AAAPPQFAPV 200
    DNSQRFTQRG GGAVGKNRRG GRGGNRGGRN NNSTRFNPLA KALGMAGESN 250
    MNFTPTKKEG RCRLFPHCPL GRSCPHAHPT KVCNEYPNCP KPPGTCEFLH 300
    PNEDEELMKE MERTREEFQK RKADLLAAKR KPVQTGIVLC KFGALCSNPS 350
    CPFGHPTPAN EDAKVIDLMW CDKNLTCDNP ECRKAHSSLS KIKEVKPISQ 400
    KKAAPPPVEK SLEQCKFGTH CTNKRCKYRH ARSHIMCREG ANCTRIDCLF 450
    GHPINEDCRF GVNCKNIYCL FRHPPGRVLP EKKGAAPNSN VPTNERPFAL 500
    PENAIIENAP PQTSFTHQEQ DTEMN 525
    Length:525
    Mass (Da):58,321
    Last modified:October 1, 1993 - v1
    Checksum:i40335C3D4658DD91
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti130 – 15728Missing in YJA512.
    Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L10288 Genomic DNA. Translation: AAA34819.1.
    L08079 Genomic DNA. Translation: AAA34820.1.
    Z72644 Genomic DNA. Translation: CAA96830.1.
    AY723810 Genomic DNA. Translation: AAU09727.1.
    BK006941 Genomic DNA. Translation: DAA07987.1.
    PIRiB48058.
    RefSeqiNP_011393.3. NM_001180987.3.

    Genome annotation databases

    EnsemblFungiiYGL122C; YGL122C; YGL122C.
    GeneIDi852755.
    KEGGisce:YGL122C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L10288 Genomic DNA. Translation: AAA34819.1 .
    L08079 Genomic DNA. Translation: AAA34820.1 .
    Z72644 Genomic DNA. Translation: CAA96830.1 .
    AY723810 Genomic DNA. Translation: AAU09727.1 .
    BK006941 Genomic DNA. Translation: DAA07987.1 .
    PIRi B48058.
    RefSeqi NP_011393.3. NM_001180987.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2JPS NMR - A 1-105 [» ]
    2LHN NMR - A 409-483 [» ]
    2V75 X-ray 1.80 A 1-104 [» ]
    3LCN X-ray 2.00 A/B 1-105 [» ]
    3ZJ1 NMR - A 253-333 [» ]
    3ZJ2 NMR - A 333-401 [» ]
    4JLQ X-ray 3.04 B 205-242 [» ]
    ProteinModelPortali P32505.
    SMRi P32505. Positions 6-94, 252-401, 404-483.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33129. 2589 interactions.
    DIPi DIP-1331N.
    IntActi P32505. 17 interactions.
    MINTi MINT-385635.
    STRINGi 4932.YGL122C.

    Proteomic databases

    MaxQBi P32505.
    PaxDbi P32505.
    PeptideAtlasi P32505.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL122C ; YGL122C ; YGL122C .
    GeneIDi 852755.
    KEGGi sce:YGL122C.

    Organism-specific databases

    CYGDi YGL122c.
    SGDi S000003090. NAB2.

    Phylogenomic databases

    eggNOGi NOG330419.
    HOGENOMi HOG000246536.
    OMAi GANCTRI.
    OrthoDBi EOG7F7WKM.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30619-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P32505.
    NextBioi 972195.

    Gene expression databases

    Genevestigatori P32505.

    Family and domain databases

    InterProi IPR021083. Poly(A)_RNA-bd_protein_2_Nab2.
    [Graphical view ]
    Pfami PF11517. Nab2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "NAB2: a yeast nuclear polyadenylated RNA-binding protein essential for cell viability."
      Anderson J.T., Wilson S.M., Datar K.V., Swanson M.S.
      Mol. Cell. Biol. 13:2730-2741(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 208279 / BJ926.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNAB2_YEAST
    AccessioniPrimary (citable) accession number: P32505
    Secondary accession number(s): D6VU26
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 9670 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3