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P32505

- NAB2_YEAST

UniProt

P32505 - NAB2_YEAST

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Protein

Nuclear polyadenylated RNA-binding protein NAB2

Gene

NAB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

This essential protein binds to polyadenylated RNA and single-stranded DNA. It may be involved not only in RNA processing but also in transcription regulation. Believed to associate directly with nascent RNA polymerase II transcripts and remain associated during subsequent nuclear RNA processing reactions.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri262 – 27817C3H1-type 1Add
BLAST
Zinc fingeri283 – 30018C3H1-type 2Add
BLAST
Zinc fingeri340 – 35516C3H1-type 3Add
BLAST
Zinc fingeri371 – 38616C3H1-type 4Add
BLAST
Zinc fingeri415 – 43016C3H1-type 5Add
BLAST
Zinc fingeri437 – 45216C3H1-type 6Add
BLAST
Zinc fingeri458 – 47316C3H1-type 7Add
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. poly(A) binding Source: SGD

GO - Biological processi

  1. mRNA polyadenylation Source: SGD
  2. poly(A)+ mRNA export from nucleus Source: SGD
  3. regulation of mRNA stability Source: SGD
Complete GO annotation...

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30619-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear polyadenylated RNA-binding protein NAB2
Gene namesi
Name:NAB2
Ordered Locus Names:YGL122C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL122c.
SGDiS000003090. NAB2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 525525Nuclear polyadenylated RNA-binding protein NAB2PRO_0000096687Add
BLAST

Proteomic databases

MaxQBiP32505.
PaxDbiP32505.
PeptideAtlasiP32505.

Expressioni

Gene expression databases

GenevestigatoriP32505.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
GFD1Q048397EBI-11770,EBI-27549
HMT1P380742EBI-11770,EBI-8394
HRP1Q993833EBI-11770,EBI-11783
MLP1Q024554EBI-11770,EBI-11009

Protein-protein interaction databases

BioGridi33129. 2589 interactions.
DIPiDIP-1331N.
IntActiP32505. 17 interactions.
MINTiMINT-385635.
STRINGi4932.YGL122C.

Structurei

Secondary structure

1
525
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 1812Combined sources
Helixi28 – 4013Combined sources
Helixi45 – 5511Combined sources
Helixi61 – 7919Combined sources
Helixi84 – 9310Combined sources
Turni269 – 2735Combined sources
Turni293 – 2953Combined sources
Beta strandi297 – 2993Combined sources
Beta strandi301 – 3033Combined sources
Helixi305 – 32622Combined sources
Beta strandi328 – 3303Combined sources
Helixi343 – 3453Combined sources
Beta strandi353 – 3553Combined sources
Helixi374 – 3763Combined sources
Beta strandi384 – 3874Combined sources
Helixi390 – 3934Combined sources
Beta strandi412 – 4143Combined sources
Helixi418 – 4203Combined sources
Beta strandi427 – 4293Combined sources
Helixi440 – 4423Combined sources
Beta strandi455 – 4573Combined sources
Beta strandi470 – 4723Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JPSNMR-A1-105[»]
2LHNNMR-A409-483[»]
2V75X-ray1.80A1-104[»]
3LCNX-ray2.00A/B1-105[»]
3ZJ1NMR-A253-333[»]
3ZJ2NMR-A333-401[»]
4JLQX-ray3.04B205-242[»]
ProteinModelPortaliP32505.
SMRiP32505. Positions 6-94, 252-401, 404-483.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32505.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati121 – 12441
Repeati125 – 12842
Repeati129 – 13243
Repeati133 – 13644
Repeati137 – 14045
Repeati141 – 14446
Repeati145 – 14847
Repeati149 – 15248
Repeati153 – 15649
Repeati157 – 160410; approximate

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni121 – 1563610 X 4 AA tandem repeats of Q-Q-Q-PAdd
BLAST
Regioni209 – 22820RNA-binding RGG-boxBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi97 – 17276Gln-richAdd
BLAST
Compositional biasi106 – 11611Poly-GlnAdd
BLAST

Sequence similaritiesi

Belongs to the NAB2 family.Curated
Contains 7 C3H1-type zinc fingers.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri262 – 27817C3H1-type 1Add
BLAST
Zinc fingeri283 – 30018C3H1-type 2Add
BLAST
Zinc fingeri340 – 35516C3H1-type 3Add
BLAST
Zinc fingeri371 – 38616C3H1-type 4Add
BLAST
Zinc fingeri415 – 43016C3H1-type 5Add
BLAST
Zinc fingeri437 – 45216C3H1-type 6Add
BLAST
Zinc fingeri458 – 47316C3H1-type 7Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG330419.
HOGENOMiHOG000246536.
InParanoidiP32505.
OMAiGANCTRI.
OrthoDBiEOG7F7WKM.

Family and domain databases

InterProiIPR021083. Poly(A)_RNA-bd_protein_2_Nab2.
[Graphical view]
PfamiPF11517. Nab2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32505-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQEQYTENL KVIVAEKLAG IPNFNEDIKY VAEYIVLLIV NGGTVESVVD
60 70 80 90 100
ELASLFDSVS RDTLANVVQT AFFALEALQQ GESAENIVSK IRMMNAQSLG
110 120 130 140 150
QSDIAQQQQQ QQQQQQPDIA QQQPQQQPQQ QPQQQPQQQP QQQPQQQPQQ
160 170 180 190 200
QPQQQPQLQP LQPQLGTQNA MQTDAPATPS PISAFSGVVN AAAPPQFAPV
210 220 230 240 250
DNSQRFTQRG GGAVGKNRRG GRGGNRGGRN NNSTRFNPLA KALGMAGESN
260 270 280 290 300
MNFTPTKKEG RCRLFPHCPL GRSCPHAHPT KVCNEYPNCP KPPGTCEFLH
310 320 330 340 350
PNEDEELMKE MERTREEFQK RKADLLAAKR KPVQTGIVLC KFGALCSNPS
360 370 380 390 400
CPFGHPTPAN EDAKVIDLMW CDKNLTCDNP ECRKAHSSLS KIKEVKPISQ
410 420 430 440 450
KKAAPPPVEK SLEQCKFGTH CTNKRCKYRH ARSHIMCREG ANCTRIDCLF
460 470 480 490 500
GHPINEDCRF GVNCKNIYCL FRHPPGRVLP EKKGAAPNSN VPTNERPFAL
510 520
PENAIIENAP PQTSFTHQEQ DTEMN
Length:525
Mass (Da):58,321
Last modified:October 1, 1993 - v1
Checksum:i40335C3D4658DD91
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti130 – 15728Missing in YJA512.
Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10288 Genomic DNA. Translation: AAA34819.1.
L08079 Genomic DNA. Translation: AAA34820.1.
Z72644 Genomic DNA. Translation: CAA96830.1.
AY723810 Genomic DNA. Translation: AAU09727.1.
BK006941 Genomic DNA. Translation: DAA07987.1.
PIRiB48058.
RefSeqiNP_011393.3. NM_001180987.3.

Genome annotation databases

EnsemblFungiiYGL122C; YGL122C; YGL122C.
GeneIDi852755.
KEGGisce:YGL122C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10288 Genomic DNA. Translation: AAA34819.1 .
L08079 Genomic DNA. Translation: AAA34820.1 .
Z72644 Genomic DNA. Translation: CAA96830.1 .
AY723810 Genomic DNA. Translation: AAU09727.1 .
BK006941 Genomic DNA. Translation: DAA07987.1 .
PIRi B48058.
RefSeqi NP_011393.3. NM_001180987.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2JPS NMR - A 1-105 [» ]
2LHN NMR - A 409-483 [» ]
2V75 X-ray 1.80 A 1-104 [» ]
3LCN X-ray 2.00 A/B 1-105 [» ]
3ZJ1 NMR - A 253-333 [» ]
3ZJ2 NMR - A 333-401 [» ]
4JLQ X-ray 3.04 B 205-242 [» ]
ProteinModelPortali P32505.
SMRi P32505. Positions 6-94, 252-401, 404-483.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33129. 2589 interactions.
DIPi DIP-1331N.
IntActi P32505. 17 interactions.
MINTi MINT-385635.
STRINGi 4932.YGL122C.

Proteomic databases

MaxQBi P32505.
PaxDbi P32505.
PeptideAtlasi P32505.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL122C ; YGL122C ; YGL122C .
GeneIDi 852755.
KEGGi sce:YGL122C.

Organism-specific databases

CYGDi YGL122c.
SGDi S000003090. NAB2.

Phylogenomic databases

eggNOGi NOG330419.
HOGENOMi HOG000246536.
InParanoidi P32505.
OMAi GANCTRI.
OrthoDBi EOG7F7WKM.

Enzyme and pathway databases

BioCyci YEAST:G3O-30619-MONOMER.

Miscellaneous databases

EvolutionaryTracei P32505.
NextBioi 972195.

Gene expression databases

Genevestigatori P32505.

Family and domain databases

InterProi IPR021083. Poly(A)_RNA-bd_protein_2_Nab2.
[Graphical view ]
Pfami PF11517. Nab2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "NAB2: a yeast nuclear polyadenylated RNA-binding protein essential for cell viability."
    Anderson J.T., Wilson S.M., Datar K.V., Swanson M.S.
    Mol. Cell. Biol. 13:2730-2741(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 208279 / BJ926.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNAB2_YEAST
AccessioniPrimary (citable) accession number: P32505
Secondary accession number(s): D6VU26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 26, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 9670 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3