ID   CBF3A_YEAST             Reviewed;         956 AA.
AC   P32504; D6VUS0; E9P954;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   14-DEC-2011, entry version 97.
DE   RecName: Full=Centromere DNA-binding protein complex CBF3 subunit A;
DE   AltName: Full=Centromere-binding factor 2;
DE   AltName: Full=Chromosome transmission fidelity protein 14;
DE   AltName: Full=Kinetochore protein CTF14;
GN   Name=CBF2; Synonyms=CBF3A, CEP2, CTF14, NDC10;
GN   OrderedLocusNames=YGR140W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RX   MEDLINE=93252988; PubMed=8486733; DOI=10.1083/jcb.121.3.513;
RA   Jiang W., Lechner J., Carbon J.;
RT   "Isolation and characterization of a gene (CBF2) specifying a protein
RT   component of the budding yeast kinetochore.";
RL   J. Cell Biol. 121:513-519(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RX   MEDLINE=93252987; PubMed=8486732; DOI=10.1083/jcb.121.3.503;
RA   Goh P.-Y., Kilmartin J.V.;
RT   "NDC10: a gene involved in chromosome segregation in Saccharomyces
RT   cerevisiae.";
RL   J. Cell Biol. 121:503-512(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 96604 / S288c / FY1679;
RX   MEDLINE=97313265; PubMed=9169869;
RA   Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M.,
RA   Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J.,
RA   Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E.,
RA   Clemente M.L., Coblenz A., Coglievina M., Coissac E., Defoor E.,
RA   Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B.,
RA   Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L.,
RA   Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M.,
RA   Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M.,
RA   Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B.,
RA   Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W.,
RA   Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A.,
RA   Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA   Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S.,
RA   Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L.,
RA   Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S.,
RA   Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J.,
RA   Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M.,
RA   Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B.,
RA   Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J.,
RA   Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M.,
RA   van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M.,
RA   Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H.,
RA   Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M.,
RA   Zollner A., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL   Nature 387:81-84(1997).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RG   Saccharomyces Genome Database;
RL   Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A.,
RA   Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F.,
RA   Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G.,
RA   Kolodner R.D., LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-
RT   encoding clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [6]
RP   INTERACTION WITH CTF19.
RX   PubMed=10323865;
RA   Ortiz J., Stemmann O., Rank S., Lechner J.;
RT   "A putative protein complex consisting of Ctf19, Mcm21, and Okp1
RT   represents a missing link in the budding yeast kinetochore.";
RL   Genes Dev. 13:1140-1155(1999).
RN   [7]
RP   SUBCELLULAR LOCATION.
RX   PubMed=10427094; DOI=10.1083/jcb.146.2.415;
RA   Zeng X., Kahana J.A., Silver P.A., Morphew M.K., McIntosh J.R.,
RA   Fitch I.T., Carbon J., Saunders W.S.;
RT   "Slk19p is a centromere protein that functions to stabilize mitotic
RT   spindles.";
RL   J. Cell Biol. 146:415-425(1999).
RN   [8]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA   Dephoure N., O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [9]
RP   ASSEMBLY OF THE CBF3 COMPLEX, AND INTERACTION WITH CBF3D AND SGT1.
RX   PubMed=15090617; DOI=10.1091/mbc.E03-12-0887;
RA   Rodrigo-Brenni M.C., Thomas S., Bouck D.C., Kaplan K.B.;
RT   "Sgt1p and Skp1p modulate the assembly and turnover of CBF3 complexes
RT   required for proper kinetochore function.";
RL   Mol. Biol. Cell 15:3366-3378(2004).
RN   [10]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-566; TYR-837 AND
RP   TYR-890, AND MASS SPECTROMETRY.
RX   PubMed=18407956; DOI=10.1074/mcp.M700468-MCP200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth
RT   phosphoproteome analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
CC   -!- FUNCTION: Acts as a component of the centromere DNA-binding
CC       protein complex CBF3, which is essential for chromosome
CC       segregation and movement of centromeres along microtubules. CBF3
CC       is required for the recruitment of other kinetochore complexes to
CC       CEN DNA. It plays a role in the attachment of chromosomes to the
CC       spindle and binds selectively to a highly conserved DNA sequence
CC       called CDEIII, found in centromers and in several promoters.
CC   -!- SUBUNIT: Component of the CBF3 copmplex, which is formed of
CC       CBF3A/CBF2, CBF3B/CEP3, CBF3C/CTF13 and CBF3D. CBF2 strongly
CC       interacts with BIR1. Interacts with the central kinetochore
CC       protein CTF19.
CC   -!- INTERACTION:
CC       P35203:CTF13; NbExp=3; IntAct=EBI-4069, EBI-4085;
CC       P53267:DAM1; NbExp=2; IntAct=EBI-4069, EBI-23268;
CC   -!- SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere. Cytoplasm,
CC       cytoskeleton, spindle pole body. Note=Spindle midzone during
CC       anaphase B.
CC   -!- MISCELLANEOUS: Present with 1350 molecules/cell in log phase SD
CC       medium.
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DR   EMBL; Z21627; CAA79753.1; -; Genomic_DNA.
DR   EMBL; X69300; CAA49158.1; -; Genomic_DNA.
DR   EMBL; Z72925; CAA97153.1; -; Genomic_DNA.
DR   EMBL; AY723816; AAU09733.1; -; Genomic_DNA.
DR   EMBL; BK006941; DAA08231.1; -; Genomic_DNA.
DR   PIR; S64449; S64449.
DR   RefSeq; NP_011656.1; NM_001181269.1.
DR   ProteinModelPortal; P32504; -.
DR   DIP; DIP-5955N; -.
DR   IntAct; P32504; 40.
DR   MINT; MINT-416776; -.
DR   STRING; P32504; -.
DR   PeptideAtlas; P32504; -.
DR   EnsemblFungi; YGR140W; YGR140W; YGR140W.
DR   GeneID; 853041; -.
DR   KEGG; sce:YGR140W; -.
DR   NMPDR; fig|4932.3.peg.2775; -.
DR   CYGD; YGR140w; -.
DR   SGD; S000003372; CBF2.
DR   eggNOG; fuNOG04656; -.
DR   HOGENOM; HBG204395; -.
DR   OMA; CVHSMNK; -.
DR   OrthoDB; EOG483HD9; -.
DR   NextBio; 972944; -.
DR   ArrayExpress; P32504; -.
DR   Genevestigator; P32504; -.
DR   GermOnline; YGR140W; Saccharomyces cerevisiae.
DR   GO; GO:0031518; C:CBF3 complex; IDA:SGD.
DR   GO; GO:0000778; C:condensed nuclear chromosome kinetochore; TAS:SGD.
DR   GO; GO:0051233; C:spindle midzone; IDA:SGD.
DR   GO; GO:0005816; C:spindle pole body; IEA:UniProtKB-SubCell.
DR   GO; GO:0019237; F:centromeric DNA binding; IDA:SGD.
DR   GO; GO:0008301; F:DNA binding, bending; IDA:SGD.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0007059; P:chromosome segregation; IMP:SGD.
DR   GO; GO:0000022; P:mitotic spindle elongation; IMP:SGD.
DR   GO; GO:0000921; P:septin ring assembly; IMP:SGD.
DR   InterPro; IPR022210; TF_OSM.
DR   KO; K11574; -.
DR   Pfam; PF12550; GCR1_C; 1.
PE   1: Evidence at protein level;
KW   Centromere; Chromosome; Complete proteome; Cytoplasm; Cytoskeleton;
KW   Direct protein sequencing; DNA-binding; Nucleus; Phosphoprotein;
KW   Reference proteome.
FT   CHAIN         1    956       Centromere DNA-binding protein complex
FT                                CBF3 subunit A.
FT                                /FTId=PRO_0000089369.
FT   MOD_RES     566    566       Phosphoserine.
FT   MOD_RES     837    837       Phosphotyrosine.
FT   MOD_RES     890    890       Phosphotyrosine.
FT   CONFLICT    418    418       T -> A (in Ref. 5; AAU09733).
FT   CONFLICT    774    774       K -> Q (in Ref. 1; CAA79753).
SQ   SEQUENCE   956 AA;  111917 MW;  C93FB7E033931E3D CRC64;
     MRSSILFLLK LMKIMDVQQQ QEAMSSEDRF QELVDSLKPR TAHQYKTYYT KYIQWCQLNQ
     IIPTPEDNSV NSVPYKDLPI SAELIHWFLL DTLITDDKPG EKREETEDLD EEEENSFKIA
     TLKKIIGSLN FLSKLCKVHE NPNANIDTKY LESVTKLHTH WIDSQKAITT NETNNTNTQV
     LCPPLLKVSL NLWNPETNHL SEKFFKTCSE KLRFLVDFQL RSYLNLSFEE RSKIRFGSLK
     LGKRDRDAII YHKVTHSAEK KDTPGHHQLL ALLPQDCPFI CPQTTLAAYL YLRFYGIPSV
     SKGDGFPNLN ADENGSLLQD IPILRGKSLT TYPREETFSN YYTTVFRYCH LPYKRREYFN
     KCNLVYPTWD EDTFRTFFNE ENHGNWLEQP EAFAFPDKIP FDFKKIMNFK SPYTSYSTNA
     KKDPFPPPKD LLVQIFPEID EYKRHDYEGL SQNSRDFLDL MEVLRERFLS NLPWIYKFFP
     NHDIFQDPIF GNSDFQSYFN DKTIHSKGSP ILSFDILPGF NKIYKNKTNF YSLLIERPSQ
     LTFASSHNPD THPTQKQESE GPLQMSQLDT TQLNELLKQQ SFEYVQFQTL SNFQILLSVF
     NKIFEKLEMK KSSRGYILHQ LNLFKITLDE RIKKSKIDDA DKFIRDNQPI KKEENIVNED
     GPNTSRRTKR PKQIRLLSIA DSSDESSTED SNVFKKDGES IEDGAYGENE DENDSEMQEQ
     LKSMINELIN SKISTFLRDQ MDQFELKINA LLDKILEEKV TRIIEQKLGS HTGKFSTLKR
     PQLYMTEEHN VGFDMEVPKK LRTSGKYAET VKDNDDHQAM STTASPSPEQ DQEAKSYTDE
     QEFMLDKSID SIEGIILEWF TPNAKYANQC VHSMNKSGNK SWRANCEALY KERKSIVEFY
     IYLVNHESLD RYKAVDICEK LRDQNEGSFS RLAKFLRKWR HDHQNSFDGL LVYLSN
//