Centromere DNA-binding protein complex CBF3 subunit A - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P32504 (CBF3A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 109. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Centromere DNA-binding protein complex CBF3 subunit A

Alternative name(s):
Centromere-binding factor 2
Chromosome transmission fidelity protein 14
Kinetochore protein CTF14

Gene names

Name:	CBF2
Synonyms:	CBF3A, CEP2, CTF14, NDC10
Ordered Locus Names:	YGR140W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	956 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromers and in several promoters.
Subunit structure	Component of the CBF3 copmplex, which is formed of CBF3A/CBF2, CBF3B/CEP3, CBF3C/CTF13 and CBF3D. CBF2 strongly interacts with BIR1. Interacts with the central kinetochore protein CTF19. Ref.6 Ref.9
Subcellular location	Nucleus. Chromosome › centromere. Cytoplasm › cytoskeleton › spindle pole body. Note: Spindle midzone during anaphase B. Ref.7
Miscellaneous	Present with 1350 molecules/cell in log phase SD medium.

Ontologies

Keywords
Cellular component	Centromere Chromosome Cytoplasm Cytoskeleton Nucleus
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	chromosome segregation Inferred from mutant phenotype Ref.2. Source: SGD mitotic spindle elongation Inferred from mutant phenotype PubMed 16381814. Source: SGD septin ring assembly Inferred from mutant phenotype PubMed 16330709. Source: SGD
Cellular_component	CBF3 complex Inferred from direct assay PubMed 1997204 PubMed 8670864 PubMed 8706132. Source: SGD condensed nuclear chromosome kinetochore Traceable author statement PubMed 10097110. Source: SGD cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW spindle midzone Inferred from direct assay PubMed 12566427. Source: SGD spindle pole body Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding, bending Inferred from direct assay PubMed 10097110. Source: SGD centromeric DNA binding Inferred from direct assay PubMed 9407032. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CTF13	P35203	3	EBI-4069,EBI-4085
DAM1	P53267	2	EBI-4069,EBI-23268

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 956

956

Centromere DNA-binding protein complex CBF3 subunit A

PRO_0000089369

Amino acid modifications

Modified residue

566

Phosphoserine Ref.10

Modified residue

837

Phosphotyrosine Ref.10

Modified residue

890

Phosphotyrosine Ref.10

Experimental info

Sequence conflict

418

T → A in AAU09733. Ref.5

Sequence conflict

774

K → Q in CAA79753. Ref.1

Secondary structure

956

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P32504 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: C93FB7E033931E3D
Show »

FASTA

956

111,917

        10         20         30         40         50         60 
MRSSILFLLK LMKIMDVQQQ QEAMSSEDRF QELVDSLKPR TAHQYKTYYT KYIQWCQLNQ 

        70         80         90        100        110        120 
IIPTPEDNSV NSVPYKDLPI SAELIHWFLL DTLITDDKPG EKREETEDLD EEEENSFKIA 

       130        140        150        160        170        180 
TLKKIIGSLN FLSKLCKVHE NPNANIDTKY LESVTKLHTH WIDSQKAITT NETNNTNTQV 

       190        200        210        220        230        240 
LCPPLLKVSL NLWNPETNHL SEKFFKTCSE KLRFLVDFQL RSYLNLSFEE RSKIRFGSLK 

       250        260        270        280        290        300 
LGKRDRDAII YHKVTHSAEK KDTPGHHQLL ALLPQDCPFI CPQTTLAAYL YLRFYGIPSV 

       310        320        330        340        350        360 
SKGDGFPNLN ADENGSLLQD IPILRGKSLT TYPREETFSN YYTTVFRYCH LPYKRREYFN 

       370        380        390        400        410        420 
KCNLVYPTWD EDTFRTFFNE ENHGNWLEQP EAFAFPDKIP FDFKKIMNFK SPYTSYSTNA 

       430        440        450        460        470        480 
KKDPFPPPKD LLVQIFPEID EYKRHDYEGL SQNSRDFLDL MEVLRERFLS NLPWIYKFFP 

       490        500        510        520        530        540 
NHDIFQDPIF GNSDFQSYFN DKTIHSKGSP ILSFDILPGF NKIYKNKTNF YSLLIERPSQ 

       550        560        570        580        590        600 
LTFASSHNPD THPTQKQESE GPLQMSQLDT TQLNELLKQQ SFEYVQFQTL SNFQILLSVF 

       610        620        630        640        650        660 
NKIFEKLEMK KSSRGYILHQ LNLFKITLDE RIKKSKIDDA DKFIRDNQPI KKEENIVNED 

       670        680        690        700        710        720 
GPNTSRRTKR PKQIRLLSIA DSSDESSTED SNVFKKDGES IEDGAYGENE DENDSEMQEQ 

       730        740        750        760        770        780 
LKSMINELIN SKISTFLRDQ MDQFELKINA LLDKILEEKV TRIIEQKLGS HTGKFSTLKR 

       790        800        810        820        830        840 
PQLYMTEEHN VGFDMEVPKK LRTSGKYAET VKDNDDHQAM STTASPSPEQ DQEAKSYTDE 

       850        860        870        880        890        900 
QEFMLDKSID SIEGIILEWF TPNAKYANQC VHSMNKSGNK SWRANCEALY KERKSIVEFY 

       910        920        930        940        950 
IYLVNHESLD RYKAVDICEK LRDQNEGSFS RLAKFLRKWR HDHQNSFDGL LVYLSN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a gene (CBF2) specifying a protein component of the budding yeast kinetochore." Jiang W., Lechner J., Carbon J. J. Cell Biol. 121:513-519(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"NDC10: a gene involved in chromosome segregation in Saccharomyces cerevisiae." Goh P.-Y., Kilmartin J.V. J. Cell Biol. 121:503-512(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"A putative protein complex consisting of Ctf19, Mcm21, and Okp1 represents a missing link in the budding yeast kinetochore." Ortiz J., Stemmann O., Rank S., Lechner J. Genes Dev. 13:1140-1155(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CTF19.
[7]	"Slk19p is a centromere protein that functions to stabilize mitotic spindles." Zeng X., Kahana J.A., Silver P.A., Morphew M.K., McIntosh J.R., Fitch I.T., Carbon J., Saunders W.S. J. Cell Biol. 146:415-425(1999) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Sgt1p and Skp1p modulate the assembly and turnover of CBF3 complexes required for proper kinetochore function." Rodrigo-Brenni M.C., Thomas S., Bouck D.C., Kaplan K.B. Mol. Biol. Cell 15:3366-3378(2004) [PubMed] [Europe PMC] [Abstract] Cited for: ASSEMBLY OF THE CBF3 COMPLEX, INTERACTION WITH CBF3D AND SGT1.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-566; TYR-837 AND TYR-890, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z21627 Genomic DNA. Translation: CAA79753.1.
X69300 Genomic DNA. Translation: CAA49158.1.
Z72925 Genomic DNA. Translation: CAA97153.1.
AY723816 Genomic DNA. Translation: AAU09733.1.
BK006941 Genomic DNA. Translation: DAA08231.1.

PIR

S64449.

RefSeq

NP_011656.3. NM_001181269.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4ACO	X-ray	1.89	A	1-956	[»]

ProteinModelPortal

P32504.

SMR

P32504. Positions 44-537.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5955N.

IntAct

P32504. 38 interactions.

MINT

MINT-416776.

STRING

4932.YGR140W.

Proteomic databases

PaxDb

P32504.

PeptideAtlas

P32504.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGR140W; YGR140W; YGR140W.

GeneID

853041.

KEGG

sce:YGR140W.
sce:YGR146C.

Organism-specific databases

CYGD

YGR140w.

SGD

S000003372. CBF2.

Phylogenomic databases

eggNOG

NOG47144.

HOGENOM

HOG000111344.

K11574.

OMA

CVHSMNK.

OrthoDB

EOG483HD9.

Gene expression databases

Genevestigator

P32504.

GermOnline

YGR140W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR022210. TF_OSM.
[Graphical view]

Pfam

PF12550. GCR1_C. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

972944.

Entry information

Entry name

CBF3A_YEAST

Accession

Primary (citable) accession number: P32504
Secondary accession number(s): D6VUS0, E9P954

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	April 3, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents