P32504 (CBF3A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Centromere DNA-binding protein complex CBF3 subunit A

Alternative name(s):
Centromere-binding factor 2
Chromosome transmission fidelity protein 14
Kinetochore protein CTF14

Gene names

Name:	CBF2
Synonyms:	CBF3A, CEP2, CTF14, NDC10
Ordered Locus Names:	YGR140W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	956 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromers and in several promoters.
Subunit structure	Component of the CBF3 copmplex, which is formed of CBF3A/CBF2, CBF3B/CEP3, CBF3C/CTF13 and CBF3D. CBF2 strongly interacts with BIR1. Interacts with the central kinetochore protein CTF19. Ref.6 Ref.9
Subcellular location	Nucleus. Chromosome › centromere. Cytoplasm › cytoskeleton › spindle pole body. Note: Spindle midzone during anaphase B. Ref.7
Miscellaneous	Present with 1350 molecules/cell in log phase SD medium. Ref.8

Ontologies

Keywords
Cellular component	Centromere Chromosome Cytoplasm Cytoskeleton Nucleus
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	chromosome segregation Inferred from mutant phenotype Ref.2. Source: SGD mitotic spindle elongation Inferred from mutant phenotype. Source: SGD septin ring assembly Inferred from mutant phenotype. Source: SGD
Cellular component	CBF3 complex Inferred from direct assay. Source: SGD condensed nuclear chromosome kinetochore Traceable author statement. Source: SGD spindle midzone Inferred from direct assay. Source: SGD spindle pole body Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding, bending Inferred from direct assay. Source: SGD centromeric DNA binding Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction Ref.9. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CTF13	P35203	3	EBI-4069,EBI-4085
DAM1	P53267	2	EBI-4069,EBI-23268

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 956	956	Centromere DNA-binding protein complex CBF3 subunit A		PRO_0000089369
Amino acid modifications
	Modified residue	566	1	Phosphoserine Ref.10
	Modified residue	837	1	Phosphotyrosine Ref.10
	Modified residue	890	1	Phosphotyrosine Ref.10
Experimental info
	Sequence conflict	418	1	T → A in AAU09733. Ref.5
	Sequence conflict	774	1	K → Q in CAA79753. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32504 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: C93FB7E033931E3D	FASTA	956	111,917
	10 20 30 40 50 60 MRSSILFLLK LMKIMDVQQQ QEAMSSEDRF QELVDSLKPR TAHQYKTYYT KYIQWCQLNQ 70 80 90 100 110 120 IIPTPEDNSV NSVPYKDLPI SAELIHWFLL DTLITDDKPG EKREETEDLD EEEENSFKIA 130 140 150 160 170 180 TLKKIIGSLN FLSKLCKVHE NPNANIDTKY LESVTKLHTH WIDSQKAITT NETNNTNTQV 190 200 210 220 230 240 LCPPLLKVSL NLWNPETNHL SEKFFKTCSE KLRFLVDFQL RSYLNLSFEE RSKIRFGSLK 250 260 270 280 290 300 LGKRDRDAII YHKVTHSAEK KDTPGHHQLL ALLPQDCPFI CPQTTLAAYL YLRFYGIPSV 310 320 330 340 350 360 SKGDGFPNLN ADENGSLLQD IPILRGKSLT TYPREETFSN YYTTVFRYCH LPYKRREYFN 370 380 390 400 410 420 KCNLVYPTWD EDTFRTFFNE ENHGNWLEQP EAFAFPDKIP FDFKKIMNFK SPYTSYSTNA 430 440 450 460 470 480 KKDPFPPPKD LLVQIFPEID EYKRHDYEGL SQNSRDFLDL MEVLRERFLS NLPWIYKFFP 490 500 510 520 530 540 NHDIFQDPIF GNSDFQSYFN DKTIHSKGSP ILSFDILPGF NKIYKNKTNF YSLLIERPSQ 550 560 570 580 590 600 LTFASSHNPD THPTQKQESE GPLQMSQLDT TQLNELLKQQ SFEYVQFQTL SNFQILLSVF 610 620 630 640 650 660 NKIFEKLEMK KSSRGYILHQ LNLFKITLDE RIKKSKIDDA DKFIRDNQPI KKEENIVNED 670 680 690 700 710 720 GPNTSRRTKR PKQIRLLSIA DSSDESSTED SNVFKKDGES IEDGAYGENE DENDSEMQEQ 730 740 750 760 770 780 LKSMINELIN SKISTFLRDQ MDQFELKINA LLDKILEEKV TRIIEQKLGS HTGKFSTLKR 790 800 810 820 830 840 PQLYMTEEHN VGFDMEVPKK LRTSGKYAET VKDNDDHQAM STTASPSPEQ DQEAKSYTDE 850 860 870 880 890 900 QEFMLDKSID SIEGIILEWF TPNAKYANQC VHSMNKSGNK SWRANCEALY KERKSIVEFY 910 920 930 940 950 IYLVNHESLD RYKAVDICEK LRDQNEGSFS RLAKFLRKWR HDHQNSFDGL LVYLSN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a gene (CBF2) specifying a protein component of the budding yeast kinetochore." Jiang W., Lechner J., Carbon J. J. Cell Biol. 121:513-519(1993) [PubMed: 8486733] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"NDC10: a gene involved in chromosome segregation in Saccharomyces cerevisiae." Goh P.-Y., Kilmartin J.V. J. Cell Biol. 121:503-512(1993) [PubMed: 8486732] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"A putative protein complex consisting of Ctf19, Mcm21, and Okp1 represents a missing link in the budding yeast kinetochore." Ortiz J., Stemmann O., Rank S., Lechner J. Genes Dev. 13:1140-1155(1999) [PubMed: 10323865] [Abstract] Cited for: INTERACTION WITH CTF19.
[7]	"Slk19p is a centromere protein that functions to stabilize mitotic spindles." Zeng X., Kahana J.A., Silver P.A., Morphew M.K., McIntosh J.R., Fitch I.T., Carbon J., Saunders W.S. J. Cell Biol. 146:415-425(1999) [PubMed: 10427094] [Abstract] Cited for: SUBCELLULAR LOCATION.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Sgt1p and Skp1p modulate the assembly and turnover of CBF3 complexes required for proper kinetochore function." Rodrigo-Brenni M.C., Thomas S., Bouck D.C., Kaplan K.B. Mol. Biol. Cell 15:3366-3378(2004) [PubMed: 15090617] [Abstract] Cited for: ASSEMBLY OF THE CBF3 COMPLEX, INTERACTION WITH CBF3D AND SGT1.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-566; TYR-837 AND TYR-890, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z21627 Genomic DNA. Translation: CAA79753.1. X69300 Genomic DNA. Translation: CAA49158.1. Z72925 Genomic DNA. Translation: CAA97153.1. AY723816 Genomic DNA. Translation: AAU09733.1. BK006941 Genomic DNA. Translation: DAA08231.1.
PIR	S64449.
RefSeq	NP_011656.1. NM_001181269.1.
3D structure databases
ProteinModelPortal	P32504.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5955N.
IntAct	P32504. 40 interactions.
MINT	MINT-416776.
STRING	P32504.
Proteomic databases
PeptideAtlas	P32504.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR140W; YGR140W; YGR140W.
GeneID	853041.
KEGG	sce:YGR140W.
NMPDR	fig|4932.3.peg.2775.
Organism-specific databases
CYGD	YGR140w.
SGD	S000003372. CBF2.
Phylogenomic databases
eggNOG	fuNOG04656.
HOGENOM	HBG204395.
OMA	CVHSMNK.
OrthoDB	EOG483HD9.
Gene expression databases
ArrayExpress	P32504.
Genevestigator	P32504.
GermOnline	YGR140W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR022210. TF_OSM. [Graphical view]
KO	K11574.
Pfam	PF12550. GCR1_C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972944.

Entry information

Entry name

CBF3A_YEAST

Accession

Primary (citable) accession number: P32504
Secondary accession number(s): D6VUS0, E9P954

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	December 14, 2011
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program