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Protein

Centromere DNA-binding protein complex CBF3 subunit A

Gene

CBF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromers and in several promoters.

Miscellaneous

Present with 1350 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • centromeric DNA binding Source: SGD
  • DNA binding, bending Source: SGD

GO - Biological processi

  • chromosome segregation Source: SGD
  • mitotic spindle elongation Source: SGD
  • septin ring assembly Source: SGD

Keywordsi

Molecular functionDNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30844-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere DNA-binding protein complex CBF3 subunit A
Alternative name(s):
Centromere-binding factor 2
Chromosome transmission fidelity protein 14
Kinetochore protein CTF14
Gene namesi
Name:CBF2
Synonyms:CBF3A, CEP2, CTF14, NDC10
Ordered Locus Names:YGR140W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR140W.
SGDiS000003372. CBF2.

Subcellular locationi

GO - Cellular componenti

  • CBF3 complex Source: SGD
  • condensed nuclear chromosome, centromeric region Source: SGD
  • condensed nuclear chromosome kinetochore Source: SGD
  • cytoplasm Source: UniProtKB-KW
  • spindle Source: SGD
  • spindle midzone Source: SGD
  • spindle pole body Source: UniProtKB-SubCell

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000893691 – 956Centromere DNA-binding protein complex CBF3 subunit AAdd BLAST956

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei566PhosphoserineCombined sources1
Modified residuei706PhosphotyrosineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32504.
PRIDEiP32504.

PTM databases

iPTMnetiP32504.

Interactioni

Subunit structurei

Component of the CBF3 copmplex, which is formed of CBF3A/CBF2, CBF3B/CEP3, CBF3C/CTF13 and CBF3D. CBF2 strongly interacts with BIR1. Interacts with the central kinetochore protein CTF19.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33386. 328 interactors.
DIPiDIP-5955N.
IntActiP32504. 39 interactors.
MINTiMINT-416776.
STRINGi4932.YGR140W.

Structurei

Secondary structure

1956
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi45 – 58Combined sources14
Turni75 – 77Combined sources3
Helixi82 – 91Combined sources10
Turni92 – 94Combined sources3
Helixi108 – 138Combined sources31
Helixi148 – 167Combined sources20
Helixi184 – 193Combined sources10
Turni198 – 200Combined sources3
Turni202 – 204Combined sources3
Helixi208 – 224Combined sources17
Helixi228 – 231Combined sources4
Helixi236 – 238Combined sources3
Beta strandi239 – 241Combined sources3
Beta strandi249 – 255Combined sources7
Beta strandi266 – 272Combined sources7
Helixi278 – 280Combined sources3
Helixi282 – 295Combined sources14
Turni298 – 300Combined sources3
Helixi309 – 311Combined sources3
Turni314 – 317Combined sources4
Helixi318 – 320Combined sources3
Beta strandi321 – 324Combined sources4
Helixi335 – 348Combined sources14
Helixi356 – 359Combined sources4
Beta strandi368 – 370Combined sources3
Helixi371 – 377Combined sources7
Helixi380 – 383Combined sources4
Helixi390 – 392Combined sources3
Helixi396 – 398Combined sources3
Helixi401 – 407Combined sources7
Helixi429 – 432Combined sources4
Helixi438 – 444Combined sources7
Helixi447 – 449Combined sources3
Helixi452 – 478Combined sources27
Helixi483 – 486Combined sources4
Helixi488 – 491Combined sources4
Helixi493 – 503Combined sources11
Helixi504 – 506Combined sources3
Turni518 – 520Combined sources3
Helixi521 – 524Combined sources4
Helixi527 – 531Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ACOX-ray1.89A1-956[»]
ProteinModelPortaliP32504.
SMRiP32504.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000111344.
InParanoidiP32504.
KOiK11574.
OMAiCVHSMNK.
OrthoDBiEOG092C37BS.

Family and domain databases

InterProiView protein in InterPro
IPR031872. NDC10_II.
IPR022210. TF_GCR1-like.
PfamiView protein in Pfam
PF12550. GCR1_C. 1 hit.
PF16787. NDC10_II. 1 hit.

Sequencei

Sequence statusi: Complete.

P32504-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSSILFLLK LMKIMDVQQQ QEAMSSEDRF QELVDSLKPR TAHQYKTYYT
60 70 80 90 100
KYIQWCQLNQ IIPTPEDNSV NSVPYKDLPI SAELIHWFLL DTLITDDKPG
110 120 130 140 150
EKREETEDLD EEEENSFKIA TLKKIIGSLN FLSKLCKVHE NPNANIDTKY
160 170 180 190 200
LESVTKLHTH WIDSQKAITT NETNNTNTQV LCPPLLKVSL NLWNPETNHL
210 220 230 240 250
SEKFFKTCSE KLRFLVDFQL RSYLNLSFEE RSKIRFGSLK LGKRDRDAII
260 270 280 290 300
YHKVTHSAEK KDTPGHHQLL ALLPQDCPFI CPQTTLAAYL YLRFYGIPSV
310 320 330 340 350
SKGDGFPNLN ADENGSLLQD IPILRGKSLT TYPREETFSN YYTTVFRYCH
360 370 380 390 400
LPYKRREYFN KCNLVYPTWD EDTFRTFFNE ENHGNWLEQP EAFAFPDKIP
410 420 430 440 450
FDFKKIMNFK SPYTSYSTNA KKDPFPPPKD LLVQIFPEID EYKRHDYEGL
460 470 480 490 500
SQNSRDFLDL MEVLRERFLS NLPWIYKFFP NHDIFQDPIF GNSDFQSYFN
510 520 530 540 550
DKTIHSKGSP ILSFDILPGF NKIYKNKTNF YSLLIERPSQ LTFASSHNPD
560 570 580 590 600
THPTQKQESE GPLQMSQLDT TQLNELLKQQ SFEYVQFQTL SNFQILLSVF
610 620 630 640 650
NKIFEKLEMK KSSRGYILHQ LNLFKITLDE RIKKSKIDDA DKFIRDNQPI
660 670 680 690 700
KKEENIVNED GPNTSRRTKR PKQIRLLSIA DSSDESSTED SNVFKKDGES
710 720 730 740 750
IEDGAYGENE DENDSEMQEQ LKSMINELIN SKISTFLRDQ MDQFELKINA
760 770 780 790 800
LLDKILEEKV TRIIEQKLGS HTGKFSTLKR PQLYMTEEHN VGFDMEVPKK
810 820 830 840 850
LRTSGKYAET VKDNDDHQAM STTASPSPEQ DQEAKSYTDE QEFMLDKSID
860 870 880 890 900
SIEGIILEWF TPNAKYANQC VHSMNKSGNK SWRANCEALY KERKSIVEFY
910 920 930 940 950
IYLVNHESLD RYKAVDICEK LRDQNEGSFS RLAKFLRKWR HDHQNSFDGL

LVYLSN
Length:956
Mass (Da):111,917
Last modified:October 1, 1993 - v1
Checksum:iC93FB7E033931E3D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti418T → A in AAU09733 (PubMed:17322287).Curated1
Sequence conflicti774K → Q in CAA79753 (PubMed:8486733).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21627 Genomic DNA. Translation: CAA79753.1.
X69300 Genomic DNA. Translation: CAA49158.1.
Z72925 Genomic DNA. Translation: CAA97153.1.
AY723816 Genomic DNA. Translation: AAU09733.1.
BK006941 Genomic DNA. Translation: DAA08231.1.
PIRiS64449.
RefSeqiNP_011656.3. NM_001181269.3.

Genome annotation databases

EnsemblFungiiYGR140W; YGR140W; YGR140W.
GeneIDi853041.
KEGGisce:YGR140W.

Similar proteinsi

Entry informationi

Entry nameiCBF3A_YEAST
AccessioniPrimary (citable) accession number: P32504
Secondary accession number(s): D6VUS0, E9P954
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 5, 2017
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names