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Protein

Translation initiation factor eIF-2B subunit beta

Gene

GCD7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a regulatory component of the translation initiation factor 2B (eIF2-B or GCD complex), which catalyzes the exchange of eukaryotic initiation factor 2 (eIF-2)-bound GDP for GTP and is regulated by phosphorylated eIF-2. It activates the synthesis of GCN4 in yeast under amino acid starvation conditions by suppressing the inhibitory effects of multiple AUG codons present in the leader of GCN4 mRNA. It may promote either repression or activation of GCN4 expression depending on amino acid availability. GCD6 and GCD7 repress GCN4 expression at the translational level by ensuring that ribosomes which have translated UORF1 will reinitiate at UORF2, -3, or -4 and thus fail to reach the GCN4 start site.2 Publications

Miscellaneous

Present with 6650 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • enzyme regulator activity Source: SGD
  • translation initiation factor activity Source: SGD

GO - Biological processi

  • regulation of translational initiation Source: SGD

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis, Translation regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-32386-MONOMER
ReactomeiR-SCE-72731 Recycling of eIF2:GDP

Names & Taxonomyi

Protein namesi
Recommended name:
Translation initiation factor eIF-2B subunit beta
Alternative name(s):
GCD complex subunit GCD7
Guanine nucleotide exchange factor subunit GCD7
eIF-2B GDP-GTP exchange factor subunit beta
Gene namesi
Name:GCD7
Synonyms:TIF222
Ordered Locus Names:YLR291C
ORF Names:L8003.17
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR291C
SGDiS000004282 GCD7

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001560661 – 381Translation initiation factor eIF-2B subunit betaAdd BLAST381

Proteomic databases

MaxQBiP32502
PaxDbiP32502
PRIDEiP32502

Interactioni

Subunit structurei

Translation initiation factor 2B (eIF2-B) is composed of five different subunits; alpha (GCN3), beta (GCD7), gamma (GCD1), delta (GCD2) and epsilon (GCD6). A regulatory subcomplex comprising GCN3, GCD7 and GCD2 interacts preferentially with phosphorylated eIF-2 and has no exchange activity in vitro.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31557, 303 interactors
DIPiDIP-1185N
IntActiP32502, 156 interactors
MINTiP32502
STRINGi4932.YLR291C

Structurei

3D structure databases

ProteinModelPortaliP32502
SMRiP32502
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074908
HOGENOMiHOG000208487
InParanoidiP32502
KOiK03754
OMAiSNAGELM
OrthoDBiEOG092C3UWH

Family and domain databases

Gene3Di1.20.120.420, 1 hit
InterProiView protein in InterPro
IPR000649 IF-2B-related
IPR027363 M1Pi_N
IPR037171 NagB/RpiA_transferase-like
PfamiView protein in Pfam
PF01008 IF-2B, 1 hit
SUPFAMiSSF100950 SSF100950, 2 hits

Sequencei

Sequence statusi: Complete.

P32502-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSQAFTSVH PNAATSDVNV TIDTFVAKLK RRQVQGSYAI ALETLQLLMR
60 70 80 90 100
FISAARWNHV NDLIEQIRDL GNSLEKAHPT AFSCGNVIRR ILAVLRDEVE
110 120 130 140 150
EDTMSTTVTS TSVAEPLISS MFNLLQKPEQ PHQNRKNSSG SSSMKTKTDY
160 170 180 190 200
RQVAIQGIKD LIDEIKNIDE GIQQIAIDLI HDHEILLTPT PDSKTVLKFL
210 220 230 240 250
ITARERSNRT FTVLVTEGFP NNTKNAHEFA KKLAQHNIET LVVPDSAVFA
260 270 280 290 300
LMSRVGKVII GTKAVFVNGG TISSNSGVSS VCECAREFRT PVFAVAGLYK
310 320 330 340 350
LSPLYPFDVE KFVEFGGSQR ILPRMDPRKR LDTVNQITDY VPPENIDIYI
360 370 380
TNVGGFNPSF IYRIAWDNYK QIDVHLDKNK A
Length:381
Mass (Da):42,570
Last modified:October 1, 1993 - v1
Checksum:iA5409FAF1594854C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07116 Genomic DNA Translation: AAA34634.1
U17243 Genomic DNA Translation: AAB67337.1
BK006945 Genomic DNA Translation: DAA09603.1
PIRiB48156
RefSeqiNP_013394.1, NM_001182179.1

Genome annotation databases

EnsemblFungiiYLR291C; YLR291C; YLR291C
GeneIDi850998
KEGGisce:YLR291C

Similar proteinsi

Entry informationi

Entry nameiEI2BB_YEAST
AccessioniPrimary (citable) accession number: P32502
Secondary accession number(s): D6VYT7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

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