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Protein

Translation initiation factor eIF-2B subunit beta

Gene

GCD7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a regulatory component of the translation initiation factor 2B (eIF2-B or GCD complex), which catalyzes the exchange of eukaryotic initiation factor 2 (eIF-2)-bound GDP for GTP and is regulated by phosphorylated eIF-2. It activates the synthesis of GCN4 in yeast under amino acid starvation conditions by suppressing the inhibitory effects of multiple AUG codons present in the leader of GCN4 mRNA. It may promote either repression or activation of GCN4 expression depending on amino acid availability. GCD6 and GCD7 repress GCN4 expression at the translational level by ensuring that ribosomes which have translated UORF1 will reinitiate at UORF2, -3, or -4 and thus fail to reach the GCN4 start site.2 Publications

Miscellaneous

Present with 6650 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • enzyme regulator activity Source: SGD
  • translation initiation factor activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis, Translation regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-32386-MONOMER.
ReactomeiR-SCE-72731. Recycling of eIF2:GDP.

Names & Taxonomyi

Protein namesi
Recommended name:
Translation initiation factor eIF-2B subunit beta
Alternative name(s):
GCD complex subunit GCD7
Guanine nucleotide exchange factor subunit GCD7
eIF-2B GDP-GTP exchange factor subunit beta
Gene namesi
Name:GCD7
Synonyms:TIF222
Ordered Locus Names:YLR291C
ORF Names:L8003.17
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR291C.
SGDiS000004282. GCD7.

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001560661 – 381Translation initiation factor eIF-2B subunit betaAdd BLAST381

Proteomic databases

MaxQBiP32502.
PRIDEiP32502.

Interactioni

Subunit structurei

Translation initiation factor 2B (eIF2-B) is composed of five different subunits; alpha (GCN3), beta (GCD7), gamma (GCD1), delta (GCD2) and epsilon (GCD6). A regulatory subcomplex comprising GCN3, GCD7 and GCD2 interacts preferentially with phosphorylated eIF-2 and has no exchange activity in vitro.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ACY3Q96HD93EBI-6260,EBI-3916242From Homo sapiens.
AIMP2Q131553EBI-6260,EBI-745226From Homo sapiens.
ATRIPQ8WXE1-33EBI-6260,EBI-11524582From a different organism.
BIRC2Q134903EBI-6260,EBI-514538From Homo sapiens.
CAMK2BQ13554-33EBI-6260,EBI-11523526From a different organism.
CASP6P552123EBI-6260,EBI-718729From Homo sapiens.
CBSP355203EBI-6260,EBI-740135From Homo sapiens.
CCDC155Q8N6L03EBI-6260,EBI-749265From Homo sapiens.
CCDC36Q8IYA83EBI-6260,EBI-8638439From Homo sapiens.
CCNDBP1O952733EBI-6260,EBI-748961From Homo sapiens.
CDC23Q9UJX23EBI-6260,EBI-396137From Homo sapiens.
CEP55Q53EZ43EBI-6260,EBI-747776From Homo sapiens.
CEP70Q8NHQ13EBI-6260,EBI-739624From Homo sapiens.
CEP70Q8NHQ1-33EBI-6260,EBI-11526150From a different organism.
CEP76Q8TAP63EBI-6260,EBI-742887From Homo sapiens.
COILP384323EBI-6260,EBI-945751From Homo sapiens.
CUTCQ9NTM93EBI-6260,EBI-714918From Homo sapiens.
DCTPP1Q9H7733EBI-6260,EBI-723569From Homo sapiens.
DNAJA3Q96EY1-33EBI-6260,EBI-11526226From a different organism.
DPH2Q9BQC33EBI-6260,EBI-10237931From Homo sapiens.
DVL2O146413EBI-6260,EBI-740850From Homo sapiens.
FAM118AQ9NWS63EBI-6260,EBI-8638992From Homo sapiens.
FBF1A0A0R4J2E43EBI-6260,EBI-11525602From Homo sapiens.
FLAD1Q8NFF5-23EBI-6260,EBI-11526128From a different organism.
GCD1P0903221EBI-6260,EBI-6275
GCD11P3248119EBI-6260,EBI-8924
GCD2P1275420EBI-6260,EBI-6265
GCD6P3250119EBI-6260,EBI-6270
GCN3P1474112EBI-6260,EBI-6253
GKAP1Q5VSY03EBI-6260,EBI-743722From Homo sapiens.
GNMTQ147493EBI-6260,EBI-744239From Homo sapiens.
GSTA4O152173EBI-6260,EBI-752440From Homo sapiens.
GSTZ1A0A0C4DFM03EBI-6260,EBI-11525587From Homo sapiens.
HGSO149643EBI-6260,EBI-740220From Homo sapiens.
HNRNPCP07910-23EBI-6260,EBI-5280084From a different organism.
HOMER3Q9NSC53EBI-6260,EBI-748420From Homo sapiens.
HOOK1Q9UJC33EBI-6260,EBI-746704From Homo sapiens.
HPRT1P004923EBI-6260,EBI-748210From Homo sapiens.
HSBP1O755063EBI-6260,EBI-748664From Homo sapiens.
HSD17B14Q9BPX13EBI-6260,EBI-742664From Homo sapiens.
KLHL12Q53G593EBI-6260,EBI-740929From Homo sapiens.
KRT15P190123EBI-6260,EBI-739566From Homo sapiens.
KRT19P087273EBI-6260,EBI-742756From Homo sapiens.
KXD1Q9BQD33EBI-6260,EBI-739657From Homo sapiens.
MAD1L1Q9Y6D93EBI-6260,EBI-742610From Homo sapiens.
MAGEA11G5E9623EBI-6260,EBI-11525615From Homo sapiens.
MAGEA12P433653EBI-6260,EBI-749530From Homo sapiens.
MAGEA2BP433563EBI-6260,EBI-5650739From Homo sapiens.
MAPRE3Q9UPY83EBI-6260,EBI-726739From Homo sapiens.
MARCH10Q8NA823EBI-6260,EBI-2341554From Homo sapiens.
MED4Q9NPJ63EBI-6260,EBI-394607From Homo sapiens.
MID2Q9UJV3-23EBI-6260,EBI-10172526From a different organism.
MIFP141743EBI-6260,EBI-372712From Homo sapiens.
MTUS2Q5JR59-33EBI-6260,EBI-11522433From a different organism.
MUK1Q028663EBI-6260,EBI-32646
MX1P205913EBI-6260,EBI-929476From Homo sapiens.
NAGKQ9UJ70-23EBI-6260,EBI-11526455From a different organism.
NECAB2Q7Z6G3-23EBI-6260,EBI-10172876From a different organism.
NME1P155313EBI-6260,EBI-741141From Homo sapiens.
NUDT21O438093EBI-6260,EBI-355720From Homo sapiens.
PAK5Q9P2863EBI-6260,EBI-741896From Homo sapiens.
PCBD1P614573EBI-6260,EBI-740475From Homo sapiens.
PDE9AO76083-23EBI-6260,EBI-11524542From a different organism.
PLSCR3Q9NRY63EBI-6260,EBI-750734From Homo sapiens.
PPCDCQ96CD23EBI-6260,EBI-724333From Homo sapiens.
PRTFDC1Q9NRG13EBI-6260,EBI-739759From Homo sapiens.
PSME3P612893EBI-6260,EBI-355546From Homo sapiens.
PTSQ033933EBI-6260,EBI-712344From Homo sapiens.
PUF60Q9UHX1-53EBI-6260,EBI-11526420From a different organism.
PUF60Q9UHX1-63EBI-6260,EBI-11085298From a different organism.
RAB3IPQ96QF0-23EBI-6260,EBI-747865From a different organism.
RABAC1Q9UI143EBI-6260,EBI-712367From Homo sapiens.
RBBP8I6L8A63EBI-6260,EBI-11525639From Homo sapiens.
REEP6Q96HR93EBI-6260,EBI-750345From Homo sapiens.
RPEQ96AT93EBI-6260,EBI-372480From Homo sapiens.
RPIAP492473EBI-6260,EBI-744831From Homo sapiens.
RTN3O95197-33EBI-6260,EBI-11525735From a different organism.
RTN4Q9NQC3-33EBI-6260,EBI-11526335From a different organism.
S100BP042713EBI-6260,EBI-458391From Homo sapiens.
SIAH1Q8IUQ4-23EBI-6260,EBI-11522811From a different organism.
SORDQ007963EBI-6260,EBI-750068From Homo sapiens.
SPERTQ8NA61-23EBI-6260,EBI-11524851From a different organism.
SPRY2O435973EBI-6260,EBI-742487From Homo sapiens.
SSSCA1O602323EBI-6260,EBI-741415From Homo sapiens.
SSX2IPQ9Y2D83EBI-6260,EBI-2212028From Homo sapiens.
STUB1Q9UNE73EBI-6260,EBI-357085From Homo sapiens.
SUI2P204596EBI-6260,EBI-8915
TCL1AP562793EBI-6260,EBI-749995From Homo sapiens.
TDO2P487753EBI-6260,EBI-743494From Homo sapiens.
TEKT1Q969V43EBI-6260,EBI-10180409From Homo sapiens.
THAP1Q9NVV93EBI-6260,EBI-741515From Homo sapiens.
TIFAQ96CG33EBI-6260,EBI-740711From Homo sapiens.
TOLLIPQ9H0E23EBI-6260,EBI-74615From Homo sapiens.
TRIM27P143733EBI-6260,EBI-719493From Homo sapiens.
TRIM32Q130493EBI-6260,EBI-742790From Homo sapiens.
TRIM69Q86WT6-23EBI-6260,EBI-11525489From a different organism.
TSC22D4Q9Y3Q83EBI-6260,EBI-739485From Homo sapiens.
UBE2IP632793EBI-6260,EBI-80168From Homo sapiens.
UBXN11Q5T124-63EBI-6260,EBI-11524408From a different organism.
WDYHV1Q96HA84EBI-6260,EBI-741158From Homo sapiens.
XIAPP981703EBI-6260,EBI-517127From Homo sapiens.
ZBED1O960063EBI-6260,EBI-740037From Homo sapiens.
ZBTB7BO15156-23EBI-6260,EBI-11522250From a different organism.
ZBTB8AQ96BR93EBI-6260,EBI-742740From Homo sapiens.
ZNF212Q9UDV63EBI-6260,EBI-1640204From Homo sapiens.

Protein-protein interaction databases

BioGridi31557. 302 interactors.
DIPiDIP-1185N.
IntActiP32502. 240 interactors.
MINTiMINT-401217.
STRINGi4932.YLR291C.

Structurei

3D structure databases

ProteinModelPortaliP32502.
SMRiP32502.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074908.
HOGENOMiHOG000208487.
InParanoidiP32502.
KOiK03754.
OMAiSNAGELM.
OrthoDBiEOG092C3UWH.

Family and domain databases

Gene3Di1.20.120.420. 1 hit.
InterProiView protein in InterPro
IPR000649. IF-2B-related.
IPR027363. M1Pi_N.
PfamiView protein in Pfam
PF01008. IF-2B. 1 hit.

Sequencei

Sequence statusi: Complete.

P32502-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSQAFTSVH PNAATSDVNV TIDTFVAKLK RRQVQGSYAI ALETLQLLMR
60 70 80 90 100
FISAARWNHV NDLIEQIRDL GNSLEKAHPT AFSCGNVIRR ILAVLRDEVE
110 120 130 140 150
EDTMSTTVTS TSVAEPLISS MFNLLQKPEQ PHQNRKNSSG SSSMKTKTDY
160 170 180 190 200
RQVAIQGIKD LIDEIKNIDE GIQQIAIDLI HDHEILLTPT PDSKTVLKFL
210 220 230 240 250
ITARERSNRT FTVLVTEGFP NNTKNAHEFA KKLAQHNIET LVVPDSAVFA
260 270 280 290 300
LMSRVGKVII GTKAVFVNGG TISSNSGVSS VCECAREFRT PVFAVAGLYK
310 320 330 340 350
LSPLYPFDVE KFVEFGGSQR ILPRMDPRKR LDTVNQITDY VPPENIDIYI
360 370 380
TNVGGFNPSF IYRIAWDNYK QIDVHLDKNK A
Length:381
Mass (Da):42,570
Last modified:October 1, 1993 - v1
Checksum:iA5409FAF1594854C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07116 Genomic DNA. Translation: AAA34634.1.
U17243 Genomic DNA. Translation: AAB67337.1.
BK006945 Genomic DNA. Translation: DAA09603.1.
PIRiB48156.
RefSeqiNP_013394.1. NM_001182179.1.

Genome annotation databases

EnsemblFungiiYLR291C; YLR291C; YLR291C.
GeneIDi850998.
KEGGisce:YLR291C.

Similar proteinsi

Entry informationi

Entry nameiEI2BB_YEAST
AccessioniPrimary (citable) accession number: P32502
Secondary accession number(s): D6VYT7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: September 27, 2017
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names