P32502 (EI2BB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Translation initiation factor eIF-2B subunit beta

Alternative name(s):
GCD complex subunit GCD7
Guanine nucleotide exchange factor subunit GCD7
eIF-2B GDP-GTP exchange factor subunit beta

Gene names

Name:	GCD7
Synonyms:	TIF222
Ordered Locus Names:	YLR291C
ORF Names:	L8003.17

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a regulatory component of the translation initiation factor 2B (eIF2-B or GCD complex), which catalyzes the exchange of eukaryotic initiation factor 2 (eIF-2)-bound GDP for GTP and is regulated by phosphorylated eIF-2. It activates the synthesis of GCN4 in yeast under amino acid starvation conditions by suppressing the inhibitory effects of multiple AUG codons present in the leader of GCN4 mRNA. It may promote either repression or activation of GCN4 expression depending on amino acid availability. GCD6 and GCD7 repress GCN4 expression at the translational level by ensuring that ribosomes which have translated UORF1 will reinitiate at UORF2, -3, or -4 and thus fail to reach the GCN4 start site. Ref.4 Ref.5
Subunit structure	Translation initiation factor 2B (eIF2-B) is composed of five different subunits; alpha (GCN3), beta (GCD7), gamma (GCD1), delta (GCD2) and epsilon (GCD6). A regulatory subcomplex comprising GCN3, GCD7 and GCD2 interacts preferentially with phosphorylated eIF-2 and has no exchange activity in vitro.
Miscellaneous	Present with 6650 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the eIF-2B alpha/beta/delta subunits family.

Ontologies

Keywords
Biological process	Protein biosynthesis Translation regulation
Molecular function	Initiation factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of translational initiation Inferred from direct assay Ref.4. Source: SGD
Cellular component	eukaryotic translation initiation factor 2B complex Inferred from direct assay Ref.4. Source: SGD guanyl-nucleotide exchange factor complex Inferred from direct assay Ref.4. Source: SGD mitochondrion Inferred from direct assay. Source: SGD
Molecular function	enzyme regulator activity Inferred from mutant phenotype. Source: SGD protein binding Inferred from physical interaction. Source: IntAct translation initiation factor activity Inferred from genetic interaction. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GCD2	P12754	7	EBI-6260,EBI-6265
GCN3	P14741	9	EBI-6260,EBI-6253
MUK1	Q02866	3	EBI-6260,EBI-32646

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	Translation initiation factor eIF-2B subunit beta		PRO_0000156066

Sequences

	Sequence		Length	Mass (Da)
	P32502 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: A5409FAF1594854C	FASTA	381	42,570
	10 20 30 40 50 60 MSSQAFTSVH PNAATSDVNV TIDTFVAKLK RRQVQGSYAI ALETLQLLMR FISAARWNHV 70 80 90 100 110 120 NDLIEQIRDL GNSLEKAHPT AFSCGNVIRR ILAVLRDEVE EDTMSTTVTS TSVAEPLISS 130 140 150 160 170 180 MFNLLQKPEQ PHQNRKNSSG SSSMKTKTDY RQVAIQGIKD LIDEIKNIDE GIQQIAIDLI 190 200 210 220 230 240 HDHEILLTPT PDSKTVLKFL ITARERSNRT FTVLVTEGFP NNTKNAHEFA KKLAQHNIET 250 260 270 280 290 300 LVVPDSAVFA LMSRVGKVII GTKAVFVNGG TISSNSGVSS VCECAREFRT PVFAVAGLYK 310 320 330 340 350 360 LSPLYPFDVE KFVEFGGSQR ILPRMDPRKR LDTVNQITDY VPPENIDIYI TNVGGFNPSF 370 380 IYRIAWDNYK QIDVHLDKNK A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence that GCD6 and GCD7, translational regulators of GCN4, are subunits of the guanine nucleotide exchange factor for eIF-2 in Saccharomyces cerevisiae." Bushman J.L., Asuru A.I., Matts R.L., Hinnebusch A.G. Mol. Cell. Biol. 13:1920-1932(1993) [PubMed: 8441423] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"A protein complex of translational regulators of GCN4 mRNA is the guanine nucleotide-exchange factor for translation initiation factor 2 in yeast." Cigan A.M., Bushman J.L., Boal T.R., Hinnebusch A.G. Proc. Natl. Acad. Sci. U.S.A. 90:5350-5354(1993) [PubMed: 8506384] [Abstract] Cited for: IDENTIFICATION IN THE EIF2-B COMPLEX, FUNCTION OF THE EIF2-B COMPLEX.
[5]	"eIF2 independently binds two distinct eIF2B subcomplexes that catalyze and regulate guanine-nucleotide exchange." Pavitt G.D., Ramaiah K.V., Kimball S.R., Hinnebusch A.G. Genes Dev. 12:514-526(1998) [PubMed: 9472020] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN A EIF2-B SUBCOMPLEX.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L07116 Genomic DNA. Translation: AAA34634.1. U17243 Genomic DNA. Translation: AAB67337.1. BK006945 Genomic DNA. Translation: DAA09603.1.
PIR	B48156.
RefSeq	NP_013394.1. NM_001182179.1.
3D structure databases
ProteinModelPortal	P32502.
SMR	P32502. Positions 16-372.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1185N.
IntAct	P32502. 138 interactions.
MINT	MINT-401217.
STRING	P32502.
Proteomic databases
PeptideAtlas	P32502.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR291C; YLR291C; YLR291C.
GeneID	850998.
KEGG	sce:YLR291C.
NMPDR	fig|4932.3.peg.4411.
Organism-specific databases
CYGD	YLR291c.
SGD	S000004282. GCD7.
Phylogenomic databases
eggNOG	fuNOG04202.
GeneTree	EFGT00070000008755.
HOGENOM	HBG318268.
OMA	RRSSEDM.
OrthoDB	EOG4D566H.
Gene expression databases
ArrayExpress	P32502.
Genevestigator	P32502.
GermOnline	YLR291C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000649. IF-2B-related. [Graphical view]
KO	K03754.
PANTHER	PTHR10233. IF-2B_related. 1 hit.
Pfam	PF01008. IF-2B. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	967537.

Entry information

Entry name

EI2BB_YEAST

Accession

Primary (citable) accession number: P32502
Secondary accession number(s): D6VYT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	December 14, 2011
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program