Translation initiation factor eIF-2B subunit beta - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P32502 (EI2BB_YEAST)

Last modified June 16, 2009. Version 69. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Translation initiation factor eIF-2B subunit beta
Alternative name(s):
    eIF-2B GDP-GTP exchange factor subunit beta
    Guanine nucleotide exchange factor subunit GCD7
    GCD complex subunit GCD7

Gene names

Name:	GCD7
Synonyms:	TIF222
Ordered Locus Names:	YLR291C
ORF Names:	L8003.17

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Acts as a regulatory component of the translation initiation factor 2B (eIF2-B or GCD complex), which catalyzes the exchange of eukaryotic initiation factor 2 (eIF-2)-bound GDP for GTP and is regulated by phosphorylated eIF-2. It activates the synthesis of GCN4 in yeast under amino acid starvation conditions by suppressing the inhibitory effects of multiple AUG codons present in the leader of GCN4 mRNA. It may promote either repression or activation of GCN4 expression depending on amino acid availability. GCD6 and GCD7 repress GCN4 expression at the translational level by ensuring that ribosomes which have translated UORF1 will reinitiate at UORF2, -3, or -4 and thus fail to reach the GCN4 start site. Ref.3 Ref.4
Subunit structure	Translation initiation factor 2B (eIF2-B) is composed of five different subunits; alpha (GCN3), beta (GCD7), gamma (GCD1), delta (GCD2) and epsilon (GCD6). A regulatory subcomplex comprising GCN3, GCD7 and GCD2 interacts preferentially with phosphorylated eIF-2 and has no exchange activity in vitro.
Miscellaneous	Present with 6650 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the EIF-2B alpha/beta/delta subunits family.

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Ontologies

Keywords
Biological process	Protein biosynthesis Translation regulation
Molecular function	Initiation factor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of translational initiation Ref.3 Inferred from direct assay. Source: SGD translation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	eukaryotic translation initiation factor 2B complex Ref.1 Ref.3 Inferred from direct assay. Source: SGD guanyl-nucleotide exchange factor complex Ref.3 Inferred from direct assay. Source: SGD mitochondrion Inferred from direct assay. Source: SGD
Molecular function	enzyme regulator activity Inferred from mutant phenotype. Source: SGD protein binding Inferred from physical interaction. Source: IntAct translation initiation factor activity Inferred from genetic interaction. Source: SGD
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
ARO8	P53090	1	EBI-6260,EBI-2042933
CAR2	P07991	1	EBI-6260,EBI-12430
CDD1	Q06549	1	EBI-6260,EBI-4455
CYS4	P32582	1	EBI-6260,EBI-4167
GCD2	P12754	2	EBI-6260,EBI-6265
GCN3	P14741	2	EBI-6260,EBI-6253
GLN1	P32288	1	EBI-6260,EBI-7665
HUA2	Q12134	1	EBI-6260,EBI-37262
IPP1	P00817	1	EBI-6260,EBI-9338
MBB1	P39534	1	EBI-6260,EBI-26211
MBF1	O14467	1	EBI-6260,EBI-2043025
MUK1	Q02866	1	EBI-6260,EBI-32646
NIP29	P33419	1	EBI-6260,EBI-12041
OYE2	Q03558	1	EBI-6260,EBI-12729
RNR1	P21524	1	EBI-6260,EBI-15234
TGL5	Q12043	1	EBI-6260,EBI-34845
THG1	P53215	1	EBI-6260,EBI-23112
VAS1	P07806-1	1	EBI-6260,EBI-1009942
VMA8	P32610	1	EBI-6260,EBI-20264

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 381

381

Translation initiation factor eIF-2B subunit beta

PRO_0000156066

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Sequences

Sequence

Length

Mass (Da)

Tools

P32502-1 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: A5409FAF1594854C
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FASTA

381

42,570

        10         20         30         40         50         60 
MSSQAFTSVH PNAATSDVNV TIDTFVAKLK RRQVQGSYAI ALETLQLLMR FISAARWNHV 

        70         80         90        100        110        120 
NDLIEQIRDL GNSLEKAHPT AFSCGNVIRR ILAVLRDEVE EDTMSTTVTS TSVAEPLISS 

       130        140        150        160        170        180 
MFNLLQKPEQ PHQNRKNSSG SSSMKTKTDY RQVAIQGIKD LIDEIKNIDE GIQQIAIDLI 

       190        200        210        220        230        240 
HDHEILLTPT PDSKTVLKFL ITARERSNRT FTVLVTEGFP NNTKNAHEFA KKLAQHNIET 

       250        260        270        280        290        300 
LVVPDSAVFA LMSRVGKVII GTKAVFVNGG TISSNSGVSS VCECAREFRT PVFAVAGLYK 

       310        320        330        340        350        360 
LSPLYPFDVE KFVEFGGSQR ILPRMDPRKR LDTVNQITDY VPPENIDIYI TNVGGFNPSF 

       370        380 
IYRIAWDNYK QIDVHLDKNK A

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence that GCD6 and GCD7, translational regulators of GCN4, are subunits of the guanine nucleotide exchange factor for eIF-2 in Saccharomyces cerevisiae." Bushman J.L., Asuru A.I., Matts R.L., Hinnebusch A.G. Mol. Cell. Biol. 13:1920-1932(1993) [PubMed: 8441423] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"A protein complex of translational regulators of GCN4 mRNA is the guanine nucleotide-exchange factor for translation initiation factor 2 in yeast." Cigan A.M., Bushman J.L., Boal T.R., Hinnebusch A.G. Proc. Natl. Acad. Sci. U.S.A. 90:5350-5354(1993) [PubMed: 8506384] [Abstract] Cited for: IDENTIFICATION IN THE EIF2-B COMPLEX, FUNCTION OF THE EIF2-B COMPLEX.
[4]	"eIF2 independently binds two distinct eIF2B subcomplexes that catalyze and regulate guanine-nucleotide exchange." Pavitt G.D., Ramaiah K.V., Kimball S.R., Hinnebusch A.G. Genes Dev. 12:514-526(1998) [PubMed: 9472020] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN A EIF2-B SUBCOMPLEX.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	L07116 Genomic DNA. Translation: AAA34634.1. U17243 Genomic DNA. Translation: AAB67337.1.
PIR	B48156.
RefSeq	NP_013394.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1185N.
IntAct	P32502. 70 interactions.
Proteomic databases
PeptideAtlas	P32502.
Genome annotation databases
Ensembl	YLR291C. Saccharomyces cerevisiae. [Contig view]
GeneID	850998.
GenomeReviews	Gene locus YLR291C in contig Y13138_GR.
KEGG	sce:YLR291C.
NMPDR	fig\|4932.3.peg.4411.
Organism-specific databases
CYGD	YLR291c.
SGD	S000004282. GCD7.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P32502.
OMA	P32502. IITDHRW.
Gene expression databases
GermOnline	YLR291C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000649. IF-2B_related. [Graphical view]
PANTHER	PTHR10233. IF-2B_related. 1 hit.
Pfam	PF01008. IF-2B. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	967537.

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Entry information

Entry name

EI2BB_YEAST

Accession

Primary (citable) accession number: P32502

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents