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Protein

Translation initiation factor eIF-2B subunit epsilon

Gene

GCD6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a catalytic component of the translation initiation factor 2B (eIF2-B or GCD complex), which catalyzes the exchange of eukaryotic initiation factor 2 (eIF-2)-bound GDP for GTP and is regulated by phosphorylated eIF-2. It activates the synthesis of GCN4 in yeast under amino acid starvation conditions by suppressing the inhibitory effects of multiple AUG codons present in the leader of GCN4 mRNA. It may promote either repression or activation of GCN4 expression depending on amino acid availability. GCD6 and GCD7 repress GCN4 expression at the translational level by ensuring that ribosomes which have translated UORF1 will reinitiate at UORF2, -3, or -4 and thus fail to reach the GCN4 start site.2 Publications

GO - Molecular functioni

  • guanyl-nucleotide exchange factor activity Source: SGD
  • nucleotidyltransferase activity Source: InterPro
  • translation initiation factor activity Source: SGD

GO - Biological processi

  • regulation of translational initiation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis, Translation regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-29793-MONOMER.
BRENDAi3.6.5.3. 984.
ReactomeiR-SCE-72731. Recycling of eIF2:GDP.

Names & Taxonomyi

Protein namesi
Recommended name:
Translation initiation factor eIF-2B subunit epsilon
Alternative name(s):
GCD complex subunit GCD6
Guanine nucleotide exchange factor subunit GCD6
eIF-2B GDP-GTP exchange factor subunit epsilon
Gene namesi
Name:GCD6
Synonyms:TIF225
Ordered Locus Names:YDR211W
ORF Names:YD8142.12, YD8142B.03
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR211W.
SGDiS000002619. GCD6.

Subcellular locationi

GO - Cellular componenti

  • eukaryotic translation initiation factor 2B complex Source: SGD
  • guanyl-nucleotide exchange factor complex Source: SGD
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi552 – 5521T → I: Reduced exchange activity. 1 Publication
Mutagenesisi569 – 5691E → A: Lethal. 1 Publication
Mutagenesisi576 – 5761S → N: Reduced exchange activity. 1 Publication
Mutagenesisi655 – 67723LFSAL…IYKWW → AFSAAVSAADNDAAEAAVAA KWA: Abolishes binding to SUI3. 1 PublicationAdd
BLAST
Mutagenesisi696 – 70611WVEWLQNADEE → AAEAAQNAAAA: Abolishes binding to SUI3; probably impairs the conversion of eIF-2-GDP to eIF-2-GTP. 1 PublicationAdd
BLAST

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 712712Translation initiation factor eIF-2B subunit epsilonPRO_0000156078Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei478 – 4781PhosphoserineCombined sources
Modified residuei481 – 4811PhosphoserineCombined sources
Modified residuei507 – 5071PhosphoserineCombined sources
Modified residuei525 – 5251PhosphoserineCombined sources
Modified residuei538 – 5381PhosphoserineCombined sources
Modified residuei707 – 7071PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32501.

PTM databases

iPTMnetiP32501.

Interactioni

Subunit structurei

Translation initiation factor 2B (eIF2-B) is composed of five different subunits; alpha (GCN3), beta (GCD7), gamma (GCD1), delta (GCD2) and epsilon (GCD6). A catalytic subcomplex comprising GCD1 and GCD6 interacts with both, phosphorylated and non-phosphorylated eIF-2 and has exchange activity in vitro. GCD6 interacts with SUI3.3 Publications

Protein-protein interaction databases

BioGridi32265. 52 interactions.
DIPiDIP-2328N.
IntActiP32501. 31 interactions.
MINTiMINT-527627.

Structurei

Secondary structure

1
712
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi545 – 55814Combined sources
Helixi563 – 57614Combined sources
Helixi581 – 60121Combined sources
Helixi607 – 61812Combined sources
Helixi619 – 6246Combined sources
Helixi629 – 64618Combined sources
Helixi651 – 66414Combined sources
Helixi670 – 6789Combined sources
Helixi684 – 6863Combined sources
Helixi687 – 70216Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PAQX-ray2.30A524-712[»]
ProteinModelPortaliP32501.
SMRiP32501. Positions 27-148, 326-435, 544-704.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32501.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini539 – 710172W2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 W2 domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00510000047568.
HOGENOMiHOG000216610.
InParanoidiP32501.
KOiK03240.
OMAiCLHYEEM.
OrthoDBiEOG092C0S7J.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
3.90.550.10. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR001451. Hexapep.
IPR016021. MIF4-like.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
IPR003307. W2_domain.
[Graphical view]
PfamiPF00132. Hexapep. 2 hits.
PF00483. NTP_transferase. 1 hit.
PF02020. W2. 1 hit.
[Graphical view]
SMARTiSM00515. eIF5C. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 2 hits.
PROSITEiPS51363. W2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32501-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKKGQKKS GLGNHGKNSD MDVEDRLQAV VLTDSYETRF MPLTAVKPRC
60 70 80 90 100
LLPLANVPLI EYTLEFLAKA GVHEVFLICS SHANQINDYI ENSKWNLPWS
110 120 130 140 150
PFKITTIMSP EARCTGDVMR DLDNRGIITG DFILVSGDVL TNIDFSKMLE
160 170 180 190 200
FHKKMHLQDK DHISTMCLSK ASTYPKTRTI EPAAFVLDKS TSRCIYYQDL
210 220 230 240 250
PLPSSREKTS IQIDPELLDN VDEFVIRNDL IDCRIDICTS HVPLIFQENF
260 270 280 290 300
DYQSLRTDFV KGVISSDILG KHIYAYLTDE YAVRVESWQT YDTISQDFLG
310 320 330 340 350
RWCYPLVLDS NIQDDQTYSY ESRHIYKEKD VVLAQSCKIG KCTAIGSGTK
360 370 380 390 400
IGEGTKIENS VIGRNCQIGE NIRIKNSFIW DDCIIGNNSI IDHSLIASNA
410 420 430 440 450
TLGSNVRLND GCIIGFNVKI DDNMDLDRNT KISASPLKNA GSRMYDNESN
460 470 480 490 500
EQFDQDLDDQ TLAVSIVGDK GVGYIYESEV SDDEDSSTEA CKEINTLSNQ
510 520 530 540 550
LDELYLSDDS ISSATKKTKK RRTMSVNSIY TDREEIDSEF EDEDFEKEGI
560 570 580 590 600
ATVERAMENN HDLDTALLEL NTLRMSMNVT YHEVRIATIT ALLRRVYHFI
610 620 630 640 650
ATQTLGPKDA VVKVFNQWGL LFKRQAFDEE EYIDLMNIIM EKIVEQSFDK
660 670 680 690 700
PDLILFSALV SLYDNDIIEE DVIYKWWDNV STDPRYDEVK KLTVKWVEWL
710
QNADEESSSE EE
Length:712
Mass (Da):81,161
Last modified:October 1, 1993 - v1
Checksum:iEFE87F6AE2941619
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07115 Genomic DNA. Translation: AAA65498.1.
Z68194 Genomic DNA. Translation: CAA92354.1.
Z68195 Genomic DNA. Translation: CAA92362.1.
BK006938 Genomic DNA. Translation: DAA12055.1.
PIRiA48156.
RefSeqiNP_010497.3. NM_001180519.3.

Genome annotation databases

EnsemblFungiiYDR211W; YDR211W; YDR211W.
GeneIDi851797.
KEGGisce:YDR211W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07115 Genomic DNA. Translation: AAA65498.1.
Z68194 Genomic DNA. Translation: CAA92354.1.
Z68195 Genomic DNA. Translation: CAA92362.1.
BK006938 Genomic DNA. Translation: DAA12055.1.
PIRiA48156.
RefSeqiNP_010497.3. NM_001180519.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PAQX-ray2.30A524-712[»]
ProteinModelPortaliP32501.
SMRiP32501. Positions 27-148, 326-435, 544-704.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32265. 52 interactions.
DIPiDIP-2328N.
IntActiP32501. 31 interactions.
MINTiMINT-527627.

PTM databases

iPTMnetiP32501.

Proteomic databases

MaxQBiP32501.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR211W; YDR211W; YDR211W.
GeneIDi851797.
KEGGisce:YDR211W.

Organism-specific databases

EuPathDBiFungiDB:YDR211W.
SGDiS000002619. GCD6.

Phylogenomic databases

GeneTreeiENSGT00510000047568.
HOGENOMiHOG000216610.
InParanoidiP32501.
KOiK03240.
OMAiCLHYEEM.
OrthoDBiEOG092C0S7J.

Enzyme and pathway databases

BioCyciYEAST:G3O-29793-MONOMER.
BRENDAi3.6.5.3. 984.
ReactomeiR-SCE-72731. Recycling of eIF2:GDP.

Miscellaneous databases

EvolutionaryTraceiP32501.
PROiP32501.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
3.90.550.10. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR001451. Hexapep.
IPR016021. MIF4-like.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
IPR003307. W2_domain.
[Graphical view]
PfamiPF00132. Hexapep. 2 hits.
PF00483. NTP_transferase. 1 hit.
PF02020. W2. 1 hit.
[Graphical view]
SMARTiSM00515. eIF5C. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 2 hits.
PROSITEiPS51363. W2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEI2BE_YEAST
AccessioniPrimary (citable) accession number: P32501
Secondary accession number(s): D6VSJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: September 7, 2016
This is version 158 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 33800 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.