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Protein

26S proteasome regulatory subunit RPN12

Gene

RPN12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Necessary for activation of the CDC28 kinase.

Miscellaneous

Present with 9890 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

Enzyme and pathway databases

BioCyciYEAST:G3O-30498-MONOMER
ReactomeiR-SCE-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-SCE-174113 SCF-beta-TrCP mediated degradation of Emi1
R-SCE-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-SCE-382556 ABC-family proteins mediated transport
R-SCE-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-SCE-5668541 TNFR2 non-canonical NF-kB pathway
R-SCE-5687128 MAPK6/MAPK4 signaling
R-SCE-5689880 Ub-specific processing proteases
R-SCE-68949 Orc1 removal from chromatin
R-SCE-69017 CDK-mediated phosphorylation and removal of Cdc6
R-SCE-69229 Ubiquitin-dependent degradation of Cyclin D1
R-SCE-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-SCE-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome regulatory subunit RPN12
Alternative name(s):
Nuclear integrity protein 1
Gene namesi
Name:RPN12
Synonyms:NIN1
Ordered Locus Names:YFR052W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFR052W
SGDiS000001948 RPN12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001738512 – 27426S proteasome regulatory subunit RPN12Add BLAST273

Proteomic databases

MaxQBiP32496
PaxDbiP32496
PRIDEiP32496

PTM databases

iPTMnetiP32496

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31210, 464 interactors
DIPiDIP-1575N
IntActiP32496, 53 interactors
MINTiP32496
STRINGi4932.YFR052W

Structurei

Secondary structure

1274
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 17Combined sources10
Helixi20 – 40Combined sources21
Helixi52 – 71Combined sources20
Helixi75 – 90Combined sources16
Helixi101 – 115Combined sources15
Helixi119 – 133Combined sources15
Turni138 – 142Combined sources5
Helixi143 – 154Combined sources12
Helixi157 – 166Combined sources10
Helixi174 – 196Combined sources23
Beta strandi198 – 201Combined sources4
Helixi202 – 209Combined sources8
Helixi215 – 223Combined sources9
Beta strandi232 – 234Combined sources3
Helixi259 – 271Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J47electron microscopy-T256-272[»]
3JCKelectron microscopy3.50H1-274[»]
3JCOelectron microscopy4.80T1-274[»]
3JCPelectron microscopy4.60T1-274[»]
4CR2electron microscopy7.70T1-274[»]
4CR3electron microscopy9.30T1-274[»]
4CR4electron microscopy8.80T1-274[»]
5A5Belectron microscopy9.50T1-274[»]
5MPBelectron microscopy7.80T1-274[»]
5MPCelectron microscopy7.70T1-274[»]
5MPDelectron microscopy4.10T1-274[»]
5MPEelectron microscopy4.50T1-274[»]
5WVIelectron microscopy6.30T1-274[»]
5WVKelectron microscopy4.20T1-274[»]
ProteinModelPortaliP32496
SMRiP32496
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini76 – 251PCIPROSITE-ProRule annotationAdd BLAST176

Sequence similaritiesi

Belongs to the proteasome subunit S14 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000014682
HOGENOMiHOG000196008
InParanoidiP32496
KOiK03031
OMAiLEIGAFW
OrthoDBiEOG092C40NW

Family and domain databases

InterProiView protein in InterPro
IPR006746 26S_Psome_Rpn12
IPR033464 CSN8_PSD8_EIF3K
PANTHERiPTHR12387 PTHR12387, 1 hit
PfamiView protein in Pfam
PF10075 CSN8_PSD8_EIF3K, 1 hit
PROSITEiView protein in PROSITE
PS50250 PCI, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32496-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSLAELTKS LSIAFENGDY AACEKLLPPI KIELIKNNLL IPDLSIQNDI
60 70 80 90 100
YLNDLMITKR ILEVGALASI QTFNFDSFEN YFNQLKPYYF SNNHKLSESD
110 120 130 140 150
KKSKLISLYL LNLLSQNNTT KFHSELQYLD KHIKNLEDDS LLSYPIKLDR
160 170 180 190 200
WLMEGSYQKA WDLLQSGSQN ISEFDSFTDI LKSAIRDEIA KNTELSYDFL
210 220 230 240 250
PLSNIKALLF FNNEKETEKF ALERNWPIVN SKVYFNNQSK EKADYEDEMM
260 270
HEEDQKTNII EKAMDYAISI ENIV
Length:274
Mass (Da):31,919
Last modified:January 23, 2007 - v3
Checksum:iD901AAD4D07ED3D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10515 Genomic DNA Translation: BAA01390.1
D50617 Genomic DNA Translation: BAA09291.1
BK006940 Genomic DNA Translation: DAA12495.1
PIRiS27434
RefSeqiNP_116710.1, NM_001180017.1

Genome annotation databases

EnsemblFungiiBAA09291; BAA09291; BAA09291
YFR052W; YFR052W; YFR052W
GeneIDi850613
KEGGisce:YFR052W

Similar proteinsi

Entry informationi

Entry nameiRPN12_YEAST
AccessioniPrimary (citable) accession number: P32496
Secondary accession number(s): D6VTT5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 152 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health