P32496 (RPN12_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
26S proteasome regulatory subunit RPN12

Alternative name(s):
Nuclear integrity protein 1

Gene names

Name:	RPN12
Synonyms:	NIN1
Ordered Locus Names:	YFR052W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Necessary for activation of the CDC28 kinase.
Miscellaneous	Present with 9890 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the proteasome subunit S14 family.

Ontologies

Keywords
Cellular component	Proteasome
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from mutant phenotype. Source: SGD
Cellular component	proteasome regulatory particle, lid subcomplex Inferred from direct assay. Source: SGD proteasome storage granule Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RPN8	Q08723	5	EBI-15953,EBI-36176
RPT3	P33298	4	EBI-15953,EBI-13905

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.6
	Chain	2 – 274	273	26S proteasome regulatory subunit RPN12		PRO_0000173851

Sequences

	Sequence		Length	Mass (Da)
	P32496 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: D901AAD4D07ED3D1	FASTA	274	31,919
	10 20 30 40 50 60 MPSLAELTKS LSIAFENGDY AACEKLLPPI KIELIKNNLL IPDLSIQNDI YLNDLMITKR 70 80 90 100 110 120 ILEVGALASI QTFNFDSFEN YFNQLKPYYF SNNHKLSESD KKSKLISLYL LNLLSQNNTT 130 140 150 160 170 180 KFHSELQYLD KHIKNLEDDS LLSYPIKLDR WLMEGSYQKA WDLLQSGSQN ISEFDSFTDI 190 200 210 220 230 240 LKSAIRDEIA KNTELSYDFL PLSNIKALLF FNNEKETEKF ALERNWPIVN SKVYFNNQSK 250 260 270 EKADYEDEMM HEEDQKTNII EKAMDYAISI ENIV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new essential gene of Saccharomyces cerevisiae, a defect in it may result in instability of nucleus." Nisogi H., Kominami K., Tanaka K., Toh-e A. Exp. Cell Res. 200:48-57(1992) [PubMed: 1563492] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Analysis of a 36.2 kb DNA sequence including the right telomere of chromosome VI from Saccharomyces cerevisiae." Eki T., Naitou M., Hagiwara H., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Hanaoka F., Murakami Y. Yeast 12:149-167(1996) [PubMed: 8686379] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	"Nin1p, a regulatory subunit of the 26S proteasome, is necessary for activation of Cdc28p kinase of Saccharomyces cerevisiae." Kominami K., DeMartino G.N., Moomaw C., Slaughter C.A., Shimbara N., Fujimuro M., Yokosawa H., Hisamatsu H., Tanahashi N., Shimizu Y., Tanaka K., Toh-e A. EMBO J. 14:3105-3115(1995) [PubMed: 7621825] [Abstract] Cited for: CHARACTERIZATION.
[6]	"N-terminal modifications of the 19S regulatory particle subunits of the yeast proteasome." Kimura Y., Saeki Y., Yokosawa H., Polevoda B., Sherman F., Hirano H. Arch. Biochem. Biophys. 409:341-348(2003) [PubMed: 12504901] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-8.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10515 Genomic DNA. Translation: BAA01390.1. D50617 Genomic DNA. Translation: BAA09291.1. BK006940 Genomic DNA. Translation: DAA12495.1.
PIR	S27434.
RefSeq	NP_116710.1. NM_001180017.1.
3D structure databases
ProteinModelPortal	P32496.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1575N.
IntAct	P32496. 40 interactions.
MINT	MINT-384687.
STRING	P32496.
Proteomic databases
PeptideAtlas	P32496.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFR052W; YFR052W; YFR052W.
GeneID	850613.
KEGG	sce:YFR052W.
NMPDR	fig|4932.3.peg.2346.
Organism-specific databases
SGD	S000001948. RPN12.
Phylogenomic databases
eggNOG	fuNOG08783.
GeneTree	EFGT00050000002355.
HOGENOM	HBG677040.
OMA	QFPINLE.
OrthoDB	EOG483HF4.
Gene expression databases
ArrayExpress	P32496.
Genevestigator	P32496.
GermOnline	YFR052W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006746. 26S_Psome_Rpn12. IPR005062. SAC3/GANP/Nin1/mts3/eIF-3-p25. [Graphical view]
KO	K03031.
PANTHER	PTHR12387. Nin1_C. 1 hit.
Pfam	PF03399. SAC3_GANP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966505.

Entry information

Entry name

RPN12_YEAST

Accession

Primary (citable) accession number: P32496
Secondary accession number(s): D6VTT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 92 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program