Reviewed, UniProtKB/Swiss-Prot P32495 (NHP2_YEAST)

Names and origin

Protein names

Recommended name:
    H/ACA ribonucleoprotein complex subunit 2
Alternative name(s):
    H/ACA snoRNP protein NHP2
    High mobility group-like nuclear protein 2

Gene names

Name:	NHP2
Ordered Locus Names:	YDL208W
ORF Names:	D1045

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ("psi") residues may serve to stabilize the conformation of rRNAs. Essential for growth. Directly binds H/ACA snoRNAs. Ref.4 Ref.5 Ref.6
Subunit structure	Component of the small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs (H/ACA snoRNPs). The protein component of the H/ACA snoRNP contains CBF5, GAR1, NHP2 and NOP10. The complex contains a stable core composed of CBF5 and NOP10, to which GAR1 and NHP2 subsequently bind. Interacts with SHQ1. Ref.7 Ref.8
Subcellular location	Nucleus › nucleolus Ref.4 Ref.9.
Sequence similarities	Belongs to the ribosomal protein L7Ae family.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Ligand	RNA-binding
Molecular function	Ribonucleoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Ref.4 Inferred from mutant phenotype. Source: SGD rRNA pseudouridine synthesis Ref.4 Inferred from mutant phenotype. Source: SGD snRNA pseudouridine synthesis Inferred from direct assay. Source: SGD
Cellular component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell small nucleolar ribonucleoprotein complex Ref.4 Ref.5 Inferred from direct assay. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction. Source: IntAct snoRNA binding Ref.6 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DBP2	P24783	1	EBI-12014,EBI-5602
DBP3	P20447	1	EBI-12014,EBI-5608
DBP6	P53734	1	EBI-12014,EBI-5625
EBP2	P36049	1	EBI-12014,EBI-6289
GAR1	P28007	1	EBI-12014,EBI-7321
NAF1	P53919	1	EBI-12014,EBI-28887
PRP43	P53131	1	EBI-12014,EBI-505
PXR1	P53335	1	EBI-12014,EBI-23652
RPA34	P47006	1	EBI-12014,EBI-26109
SSF1	P38789	1	EBI-12014,EBI-18160

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 156	156	H/ACA ribonucleoprotein complex subunit 2		PRO_0000136776
Experimental info
	Mutagenesis	56	1	V → K: No effect. Ref.6
	Mutagenesis	59	1	G → E: Significant growth impairment at 30 and 37 degrees Celsius. Impaired association with H/ACA snoRNAs. Ref.6
	Mutagenesis	68	1	R → A: No effect. Ref.6
	Mutagenesis	76 – 77	2	Missing: Lethal. Impaired association with H/ACA snoRNAs. Accumulation of NHP2 within the nucleolus.
	Mutagenesis	80	1	D → A: No effect. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P32495-1 [UniParc]. Last modified February 1, 2005. Version 2. Checksum: 008188132B494E5D	FASTA	156	17,122
	10 20 30 40 50 60 MGKDNKEHKE SKESKTVDNY EARMPAVLPF AKPLASKKLN KKVLKTVKKA SKAKNVKRGV 70 80 90 100 110 120 KEVVKALRKG EKGLVVIAGD ISPADVISHI PVLCEDHSVP YIFIPSKQDL GAAGATKRPT 130 140 150 SVVFIVPGSN KKKDGKNKEE EYKESFNEVV KEVQAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and genetic analysis of NHP2: a moderately abundant high mobility group-like nuclear protein with an essential function in Saccharomyces cerevisiae." Kolodrubetz D., Burgum A. Yeast 7:79-90(1991) [PubMed: 2063628] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of a 39 kb segment of yeast chromosome IV: 12 new open reading frames, nine known genes and one gene for Gly-tRNA." Bahr A., Moeller-Rieker S., Hankeln T., Kraemer C., Protin U., Schmidt E.R. Yeast 13:163-169(1997) [PubMed: 9046097] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Nhp2p and Nop10p are essential for the function of H/ACA snoRNPs." Henras A., Henry Y., Bousquet-Antonelli C., Noaillac-Depeyre J., Gelugne J.-P., Caizergues-Ferrer M. EMBO J. 17:7078-7090(1998) [PubMed: 9843512] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-9, FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN H/ACA SNORNP COMPLEXES.
[5]	"Cbf5p, a potential pseudouridine synthase, and Nhp2p, a putative RNA-binding protein, are present together with Gar1p in all H BOX/ACA-motif snoRNPs and constitute a common bipartite structure." Watkins N.J., Gottschalk A., Neubauer G., Kastner B., Fabrizio P., Mann M., Luehrmann R. RNA 4:1549-1568(1998) [PubMed: 9848653] [Abstract] Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN H/ACA SNORNP COMPLEXES.
[6]	"Accumulation of H/ACA snoRNPs depends on the integrity of the conserved central domain of the RNA-binding protein Nhp2p." Henras A., Dez C., Noaillac-Depeyre J., Henry Y., Caizergues-Ferrer M. Nucleic Acids Res. 29:2733-2746(2001) [PubMed: 11433018] [Abstract] Cited for: FUNCTION, RNA-BINDING, MUTAGENESIS OF VAL-56; GLY-59; ARG-68; 76-VAL-ILE-77 AND ASP-80.
[7]	"The Shq1p.Naf1p complex is required for box H/ACA small nucleolar ribonucleoprotein particle biogenesis." Yang P.K., Rotondo G., Porras T., Legrain P., Chanfreau G. J. Biol. Chem. 277:45235-45242(2002) [PubMed: 12228251] [Abstract] Cited for: INTERACTION WITH SHQ1.
[8]	"Naf1 p is a box H/ACA snoRNP assembly factor." Fatica A., Dlakic M., Tollervey D. RNA 8:1502-1514(2002) [PubMed: 12515383] [Abstract] Cited for: INTERACTION WITH NAF1.
[9]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"Cbf5p, the putative pseudouridine synthase of H/ACA-type snoRNPs, can form a complex with Gar1p and Nop10p in absence of Nhp2p and box H/ACA snoRNAs." Henras A.K., Capeyrou R., Henry Y., Caizergues-Ferrer M. RNA 10:1704-1712(2004) [PubMed: 15388873] [Abstract] Cited for: CHARACTERIZATION OF THE H/ACA SNORNP COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X57714 Genomic DNA. Translation: CAA40885.1. Different initiation. X99000 Genomic DNA. Translation: CAA67483.1. Different initiation. Z74256 Genomic DNA. Translation: CAA98786.1. Different initiation.
PIR	S67767.
RefSeq	NP_010073.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5134N.
IntAct	P32495. 52 interactions.
STRING	P32495.
Proteomic databases
PeptideAtlas	P32495.
PRIDE	P32495.
Genome annotation databases
Ensembl	YDL208W; YDL208W; YDL208W; Saccharomyces cerevisiae. [Genome view]
GeneID	851319.
KEGG	sce:YDL208W.
Organism-specific databases
CYGD	YDL208w.
SGD	S000002367. NHP2.
Phylogenomic databases
HOGENOM	HBG739027.
OrthoDB	EOG973R7R.
Gene expression databases
ArrayExpress	P32495.
Genevestigator	P32495.
GermOnline	YDL208W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002415. HMG_nuclear. IPR018492. Ribosomal_L7A/RS6_fam. IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45. IPR004037. Ribosomal_L7Ae_CS. [Graphical view]
Pfam	PF01248. Ribosomal_L7Ae. 1 hit. [Graphical view]
PRINTS	PR00881. L7ARS6FAMILY. PR00883. NUCLEARHMG.
PROSITE	PS01082. RIBOSOMAL_L7AE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	968357.

Entry information

Entry name

NHP2_YEAST

Accession

Primary (citable) accession number: P32495

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	February 1, 2005
Last modified:	December 15, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)