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P32492

- MYO4_YEAST

UniProt

P32492 - MYO4_YEAST

Protein

Myosin-4

Gene

MYO4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Recruited to specific mRNAs including the ASH1 mRNA, coding for a repressor of the HO endonuclease, via its interaction with SHE3.6 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi165 – 1728ATPSequence Analysis

    GO - Molecular functioni

    1. actin filament binding Source: SGD
    2. ATP binding Source: UniProtKB-KW
    3. microfilament motor activity Source: SGD
    4. protein binding Source: IntAct

    GO - Biological processi

    1. endoplasmic reticulum inheritance Source: SGD
    2. intracellular mRNA localization Source: SGD
    3. mating type switching Source: SGD
    4. mRNA transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    Motor protein, Myosin

    Keywords - Biological processi

    mRNA transport, Transport

    Keywords - Ligandi

    Actin-binding, ATP-binding, Calmodulin-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28840-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myosin-4
    Alternative name(s):
    SWI5-dependent HO expression protein 1
    Gene namesi
    Name:MYO4
    Synonyms:SHE1
    Ordered Locus Names:YAL029C
    ORF Names:FUN22
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome I

    Organism-specific databases

    CYGDiYAL029c.
    SGDiS000000027. MYO4.

    Subcellular locationi

    Bud 1 Publication
    Note: Accumulates preferentially in growing buds.

    GO - Cellular componenti

    1. cellular bud Source: SGD
    2. cellular bud tip Source: SGD
    3. filamentous actin Source: SGD
    4. myosin V complex Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 14711471Myosin-4PRO_0000123491Add
    BLAST

    Proteomic databases

    MaxQBiP32492.
    PaxDbiP32492.
    PeptideAtlasiP32492.

    Expressioni

    Gene expression databases

    GenevestigatoriP32492.

    Interactioni

    Subunit structurei

    Interacts with SHE2 and SHE3.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SHE2P360684EBI-11681,EBI-26866
    SHE3P382727EBI-11681,EBI-21600

    Protein-protein interaction databases

    BioGridi31737. 55 interactions.
    DIPiDIP-5761N.
    IntActiP32492. 31 interactions.
    MINTiMINT-617203.
    STRINGi4932.YAL029C.

    Structurei

    Secondary structure

    1
    1471
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi1104 – 11096
    Helixi1111 – 112111
    Helixi1128 – 11303
    Helixi1138 – 115215
    Helixi1156 – 117621
    Turni1179 – 11813
    Helixi1182 – 119312
    Helixi1196 – 12049
    Helixi1205 – 12073
    Helixi1217 – 125337
    Helixi1255 – 12595
    Helixi1262 – 12643
    Beta strandi1265 – 12673
    Helixi1270 – 128920
    Helixi1294 – 131926
    Helixi1325 – 134218
    Turni1343 – 13453
    Helixi1350 – 13534
    Helixi1354 – 136411
    Helixi1370 – 13778
    Helixi1385 – 13939
    Beta strandi1398 – 14014
    Helixi1407 – 142317
    Helixi1440 – 14423
    Helixi1458 – 14647

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3MMIX-ray2.30A/B1091-1471[»]
    4LL6X-ray2.30A1098-1471[»]
    4LL8X-ray3.58A918-1471[»]
    ProteinModelPortaliP32492.
    SMRiP32492. Positions 4-840, 1016-1468.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP32492.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini71 – 777707Myosin motorAdd
    BLAST
    Domaini781 – 80121IQ 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini804 – 82421IQ 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini829 – 84921IQ 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini876 – 89823IQ 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini899 – 92830IQ 5PROSITE-ProRule annotationAdd
    BLAST
    Domaini1164 – 1419256DilutePROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili938 – 1063126Add
    BLAST

    Sequence similaritiesi

    Contains 1 dilute domain.PROSITE-ProRule annotation
    Contains 5 IQ domains.PROSITE-ProRule annotation
    Contains 1 myosin motor domain.Curated

    Keywords - Domaini

    Coiled coil, Repeat

    Phylogenomic databases

    eggNOGiCOG5022.
    GeneTreeiENSGT00750000117369.
    HOGENOMiHOG000171839.
    KOiK10357.
    OMAiAEENTIV.
    OrthoDBiEOG77T1CS.

    Family and domain databases

    InterProiIPR018444. Dil_domain.
    IPR002710. Dilute.
    IPR000048. IQ_motif_EF-hand-BS.
    IPR001609. Myosin_head_motor_dom.
    IPR008989. Myosin_S1_N.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF01843. DIL. 1 hit.
    PF00612. IQ. 1 hit.
    PF00063. Myosin_head. 1 hit.
    [Graphical view]
    PRINTSiPR00193. MYOSINHEAVY.
    SMARTiSM00015. IQ. 4 hits.
    SM00242. MYSc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50084. SSF50084. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS51126. DILUTE. 1 hit.
    PS50096. IQ. 2 hits.
    PS51456. MYOSIN_MOTOR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32492-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSFEVGTKCW YPHKEQGWIG GEVTKNDFFE GTFHLELKLE DGETVSIETN     50
    SFENDDDHPT LPVLRNPPIL ESTDDLTTLS YLNEPAVLHA IKKRYMNGQI 100
    YTYSGIVLIA ANPFDKVDHL YSREMIQNYS SKRKDELEPH LFAIAEEAYR 150
    FMVHEKANQT VVVSGESGAG KTVSAKYIMR YFASVQESNN REGEVEMSQI 200
    ESQILATNPI MEAFGNAKTT RNDNSSRFGK YLQILFDENT TIRGSKIRTY 250
    LLEKSRLVYQ PETERNYHIF YQILEGLPEP VKQELHLSSP KDYHYTNQGG 300
    QPNIAGIDEA REYKITTDAL SLVGINHETQ LGIFKILAGL LHIGNIEMKM 350
    TRNDASLSSE EQNLQIACEL LGIDPFNFAK WIVKKQIVTR SEKIVTNLNY 400
    NQALIARDSV AKFIYSTLFD WLVDNINKTL YDPELDQQDH VFSFIGILDI 450
    YGFEHFEKNS FEQFCINYAN EKLQQEFNQH VFKLEQEEYV KEEIEWSFIE 500
    FSDNQPCIDL IENKLGILSL LDEESRLPSG SDESWASKLY SAFNKPPSNE 550
    VFSKPRFGQT KFIVSHYAVD VEYEVEGFIE KNRDSVSLGH LDVFKATTNP 600
    IFKQILDNRE LRSDDAPEEQ NTEKKIMIPA RLSQKKPTLG SMFKKSLGEL 650
    MAIINSTNVH YIRCIKPNSE KKPWEFDNLM VLSQLRACGV LETIRISCAG 700
    FPSRWTFDEF VQRYFLLTDY SLWSGILYNP DLPKEAIVNF CQSILDATIS 750
    DSAKYQIGNT KIFFKAGMLA FLEKLRTNKM NEICIIIQKK IRARYYRLQY 800
    LQTMESIKKC QSQIRSLLVR TRVDHELKTR AAILLQTNIR ALWKREYYRA 850
    AIGQIIKLQC TCKRKLILDS VNRKFMLMAA VIIQSYIRSY GHKTDYRTLK 900
    RSSILVQSAM RMQLARRRYI VLQKEVEERN IRASYGIGLL EEAIEFKNSF 950
    ILNLEMLNDS YTRLTQLLQG DLSNIPSKQR QEYETIVNGY NDKISKLKTL 1000
    QVEIMNTLNK KNALKERKKK QSSLIQSHMQ SLAAIKGNKP SRLSDEVKSM 1050
    KQELAFIENV IAQDFTTTYS ANKNDKVKGL GIAGQQVKPK LVNVIRRESG 1100
    NPDLLELLMD LNCYTLEVTE GYLKKVNVTE VNGDNVLGPI HVITTVVSSL 1150
    VRNGLLIQSS KFISKVLLTV ESIVMSLPKD ETMLGGIFWL SNLSRLPAFA 1200
    ANQKTLYEAN GGDEKDKLTL IYLNDLENET LKVFDKIYST WLVKFMKHAS 1250
    AHIEIFDMVL NEKLFKNSGD EKFAKLFTFL NEFDAVLCKF QVVDSMHTKI 1300
    FNDTLKYLNV MLFNDLITKC PALNWKYGYE VDRNIERLVS WFEPRIEDVR 1350
    PNLIQIIQAV KILQLKISNL NEFKLLFDFW YALNPAQIQA ILLKYKPANK 1400
    GEAGVPNEIL NYLANVIKRE NLSLPGKMEI MLSAQFDSAK NHLRYDTSAI 1450
    TQNSNTEGLA TVSKIIKLDR K 1471
    Length:1,471
    Mass (Da):169,344
    Last modified:October 1, 1993 - v1
    Checksum:iE79C0FE72B041E95
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M90057 Unassigned DNA. Translation: AAC37409.1.
    U12980 Genomic DNA. Translation: AAC05003.1.
    BK006935 Genomic DNA. Translation: DAA06959.1.
    PIRiS30790.
    RefSeqiNP_009373.1. NM_001178174.1.

    Genome annotation databases

    EnsemblFungiiYAL029C; YAL029C; YAL029C.
    GeneIDi851204.
    KEGGisce:YAL029C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M90057 Unassigned DNA. Translation: AAC37409.1 .
    U12980 Genomic DNA. Translation: AAC05003.1 .
    BK006935 Genomic DNA. Translation: DAA06959.1 .
    PIRi S30790.
    RefSeqi NP_009373.1. NM_001178174.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3MMI X-ray 2.30 A/B 1091-1471 [» ]
    4LL6 X-ray 2.30 A 1098-1471 [» ]
    4LL8 X-ray 3.58 A 918-1471 [» ]
    ProteinModelPortali P32492.
    SMRi P32492. Positions 4-840, 1016-1468.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31737. 55 interactions.
    DIPi DIP-5761N.
    IntActi P32492. 31 interactions.
    MINTi MINT-617203.
    STRINGi 4932.YAL029C.

    Proteomic databases

    MaxQBi P32492.
    PaxDbi P32492.
    PeptideAtlasi P32492.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YAL029C ; YAL029C ; YAL029C .
    GeneIDi 851204.
    KEGGi sce:YAL029C.

    Organism-specific databases

    CYGDi YAL029c.
    SGDi S000000027. MYO4.

    Phylogenomic databases

    eggNOGi COG5022.
    GeneTreei ENSGT00750000117369.
    HOGENOMi HOG000171839.
    KOi K10357.
    OMAi AEENTIV.
    OrthoDBi EOG77T1CS.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28840-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P32492.
    NextBioi 968067.

    Gene expression databases

    Genevestigatori P32492.

    Family and domain databases

    InterProi IPR018444. Dil_domain.
    IPR002710. Dilute.
    IPR000048. IQ_motif_EF-hand-BS.
    IPR001609. Myosin_head_motor_dom.
    IPR008989. Myosin_S1_N.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF01843. DIL. 1 hit.
    PF00612. IQ. 1 hit.
    PF00063. Myosin_head. 1 hit.
    [Graphical view ]
    PRINTSi PR00193. MYOSINHEAVY.
    SMARTi SM00015. IQ. 4 hits.
    SM00242. MYSc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50084. SSF50084. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS51126. DILUTE. 1 hit.
    PS50096. IQ. 2 hits.
    PS51456. MYOSIN_MOTOR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of MYO4, a second class V myosin gene in yeast."
      Haarer B.K., Petzold A., Lillie S.H., Brown S.S.
      J. Cell Sci. 107:1055-1064(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Association of the class V myosin Myo4p with a localised messenger RNA in budding yeast depends on She proteins."
      Munchow S., Sauter C., Jansen R.P.
      J. Cell Sci. 112:1511-1518(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "She2p, a novel RNA-binding protein tethers ASH1 mRNA to the Myo4p myosin motor via She3p."
      Bohl F., Kruse C., Frank A., Ferring D., Jansen R.P.
      EMBO J. 19:5514-5524(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SHE2 AND SHE3.
    6. "She2p is a novel RNA-binding protein that recruits the Myo4p-She3p complex to ASH1 mRNA."
      Long R.M., Gu W., Lorimer E., Singer R.H., Chartrand P.
      EMBO J. 19:6592-6601(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SHE3.
    7. "The myosin motor, Myo4p, binds Ash1 mRNA via the adapter protein, She3p."
      Takizawa P.A., Vale R.D.
      Proc. Natl. Acad. Sci. U.S.A. 97:5273-5278(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SHE2 AND SHE3.
    8. "Ribonucleoprotein-dependent localization of the yeast class V myosin Myo4p."
      Kruse C., Jaedicke A., Beaudouin J., Bohl F., Ferring D., Guttler T., Ellenberg J., Jansen R.P.
      J. Cell Biol. 159:971-982(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "ASH1 mRNA anchoring requires reorganization of the Myo4p-She3p-She2p transport complex."
      Gonsalvez G.B., Little J.L., Long R.M.
      J. Biol. Chem. 279:46286-46294(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "The structure of the Myo4p globular tail and its function in ASH1 mRNA localization."
      Heuck A., Fetka I., Brewer D.N., Huls D., Munson M., Jansen R.P., Niessing D.
      J. Cell Biol. 189:497-510(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 1091-1471, FUNCTION, INTERACTION WITH SHE3.

    Entry informationi

    Entry nameiMYO4_YEAST
    AccessioniPrimary (citable) accession number: P32492
    Secondary accession number(s): D6VPI9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 134 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2210 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome I
      Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

    External Data

    Dasty 3