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P32492

- MYO4_YEAST

UniProt

P32492 - MYO4_YEAST

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Protein

Myosin-4

Gene

MYO4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Recruited to specific mRNAs including the ASH1 mRNA, coding for a repressor of the HO endonuclease, via its interaction with SHE3.6 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi165 – 1728ATPSequence Analysis

GO - Molecular functioni

  1. actin filament binding Source: SGD
  2. ATP binding Source: UniProtKB-KW
  3. microfilament motor activity Source: SGD

GO - Biological processi

  1. endoplasmic reticulum inheritance Source: SGD
  2. intracellular mRNA localization Source: SGD
  3. mating type switching Source: SGD
  4. mRNA transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Myosin

Keywords - Biological processi

mRNA transport, Transport

Keywords - Ligandi

Actin-binding, ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28840-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-4
Alternative name(s):
SWI5-dependent HO expression protein 1
Gene namesi
Name:MYO4
Synonyms:SHE1
Ordered Locus Names:YAL029C
ORF Names:FUN22
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

CYGDiYAL029c.
SGDiS000000027. MYO4.

Subcellular locationi

Bud 1 Publication
Note: Accumulates preferentially in growing buds.

GO - Cellular componenti

  1. cellular bud Source: SGD
  2. cellular bud tip Source: SGD
  3. filamentous actin Source: SGD
  4. myosin V complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14711471Myosin-4PRO_0000123491Add
BLAST

Proteomic databases

MaxQBiP32492.
PaxDbiP32492.
PeptideAtlasiP32492.

Expressioni

Gene expression databases

GenevestigatoriP32492.

Interactioni

Subunit structurei

Interacts with SHE2 and SHE3.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SHE2P360684EBI-11681,EBI-26866
SHE3P382727EBI-11681,EBI-21600

Protein-protein interaction databases

BioGridi31737. 55 interactions.
DIPiDIP-5761N.
IntActiP32492. 31 interactions.
MINTiMINT-617203.
STRINGi4932.YAL029C.

Structurei

Secondary structure

1
1471
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1104 – 11096
Helixi1111 – 112111
Helixi1128 – 11303
Helixi1138 – 115215
Helixi1156 – 117621
Turni1179 – 11813
Helixi1182 – 119312
Helixi1196 – 12049
Helixi1205 – 12073
Helixi1217 – 125337
Helixi1255 – 12595
Helixi1262 – 12643
Beta strandi1265 – 12673
Helixi1270 – 128920
Helixi1294 – 131926
Helixi1325 – 134218
Turni1343 – 13453
Helixi1350 – 13534
Helixi1354 – 136411
Helixi1370 – 13778
Helixi1385 – 13939
Beta strandi1398 – 14014
Helixi1407 – 142317
Helixi1440 – 14423
Helixi1458 – 14647

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MMIX-ray2.30A/B1091-1471[»]
4LL6X-ray2.30A1098-1471[»]
4LL8X-ray3.58A918-1471[»]
ProteinModelPortaliP32492.
SMRiP32492. Positions 4-840, 1016-1468.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32492.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini71 – 777707Myosin motorAdd
BLAST
Domaini781 – 80121IQ 1PROSITE-ProRule annotationAdd
BLAST
Domaini804 – 82421IQ 2PROSITE-ProRule annotationAdd
BLAST
Domaini829 – 84921IQ 3PROSITE-ProRule annotationAdd
BLAST
Domaini876 – 89823IQ 4PROSITE-ProRule annotationAdd
BLAST
Domaini899 – 92830IQ 5PROSITE-ProRule annotationAdd
BLAST
Domaini1164 – 1419256DilutePROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili938 – 1063126Add
BLAST

Sequence similaritiesi

Contains 1 dilute domain.PROSITE-ProRule annotation
Contains 5 IQ domains.PROSITE-ProRule annotation
Contains 1 myosin motor domain.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiCOG5022.
GeneTreeiENSGT00760000119299.
HOGENOMiHOG000171839.
InParanoidiP32492.
KOiK10357.
OMAiAEENTIV.
OrthoDBiEOG77T1CS.

Family and domain databases

InterProiIPR018444. Dil_domain.
IPR002710. Dilute.
IPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR008989. Myosin_S1_N.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01843. DIL. 1 hit.
PF00612. IQ. 1 hit.
PF00063. Myosin_head. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00015. IQ. 4 hits.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF50084. SSF50084. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51126. DILUTE. 1 hit.
PS50096. IQ. 2 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32492-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSFEVGTKCW YPHKEQGWIG GEVTKNDFFE GTFHLELKLE DGETVSIETN
60 70 80 90 100
SFENDDDHPT LPVLRNPPIL ESTDDLTTLS YLNEPAVLHA IKKRYMNGQI
110 120 130 140 150
YTYSGIVLIA ANPFDKVDHL YSREMIQNYS SKRKDELEPH LFAIAEEAYR
160 170 180 190 200
FMVHEKANQT VVVSGESGAG KTVSAKYIMR YFASVQESNN REGEVEMSQI
210 220 230 240 250
ESQILATNPI MEAFGNAKTT RNDNSSRFGK YLQILFDENT TIRGSKIRTY
260 270 280 290 300
LLEKSRLVYQ PETERNYHIF YQILEGLPEP VKQELHLSSP KDYHYTNQGG
310 320 330 340 350
QPNIAGIDEA REYKITTDAL SLVGINHETQ LGIFKILAGL LHIGNIEMKM
360 370 380 390 400
TRNDASLSSE EQNLQIACEL LGIDPFNFAK WIVKKQIVTR SEKIVTNLNY
410 420 430 440 450
NQALIARDSV AKFIYSTLFD WLVDNINKTL YDPELDQQDH VFSFIGILDI
460 470 480 490 500
YGFEHFEKNS FEQFCINYAN EKLQQEFNQH VFKLEQEEYV KEEIEWSFIE
510 520 530 540 550
FSDNQPCIDL IENKLGILSL LDEESRLPSG SDESWASKLY SAFNKPPSNE
560 570 580 590 600
VFSKPRFGQT KFIVSHYAVD VEYEVEGFIE KNRDSVSLGH LDVFKATTNP
610 620 630 640 650
IFKQILDNRE LRSDDAPEEQ NTEKKIMIPA RLSQKKPTLG SMFKKSLGEL
660 670 680 690 700
MAIINSTNVH YIRCIKPNSE KKPWEFDNLM VLSQLRACGV LETIRISCAG
710 720 730 740 750
FPSRWTFDEF VQRYFLLTDY SLWSGILYNP DLPKEAIVNF CQSILDATIS
760 770 780 790 800
DSAKYQIGNT KIFFKAGMLA FLEKLRTNKM NEICIIIQKK IRARYYRLQY
810 820 830 840 850
LQTMESIKKC QSQIRSLLVR TRVDHELKTR AAILLQTNIR ALWKREYYRA
860 870 880 890 900
AIGQIIKLQC TCKRKLILDS VNRKFMLMAA VIIQSYIRSY GHKTDYRTLK
910 920 930 940 950
RSSILVQSAM RMQLARRRYI VLQKEVEERN IRASYGIGLL EEAIEFKNSF
960 970 980 990 1000
ILNLEMLNDS YTRLTQLLQG DLSNIPSKQR QEYETIVNGY NDKISKLKTL
1010 1020 1030 1040 1050
QVEIMNTLNK KNALKERKKK QSSLIQSHMQ SLAAIKGNKP SRLSDEVKSM
1060 1070 1080 1090 1100
KQELAFIENV IAQDFTTTYS ANKNDKVKGL GIAGQQVKPK LVNVIRRESG
1110 1120 1130 1140 1150
NPDLLELLMD LNCYTLEVTE GYLKKVNVTE VNGDNVLGPI HVITTVVSSL
1160 1170 1180 1190 1200
VRNGLLIQSS KFISKVLLTV ESIVMSLPKD ETMLGGIFWL SNLSRLPAFA
1210 1220 1230 1240 1250
ANQKTLYEAN GGDEKDKLTL IYLNDLENET LKVFDKIYST WLVKFMKHAS
1260 1270 1280 1290 1300
AHIEIFDMVL NEKLFKNSGD EKFAKLFTFL NEFDAVLCKF QVVDSMHTKI
1310 1320 1330 1340 1350
FNDTLKYLNV MLFNDLITKC PALNWKYGYE VDRNIERLVS WFEPRIEDVR
1360 1370 1380 1390 1400
PNLIQIIQAV KILQLKISNL NEFKLLFDFW YALNPAQIQA ILLKYKPANK
1410 1420 1430 1440 1450
GEAGVPNEIL NYLANVIKRE NLSLPGKMEI MLSAQFDSAK NHLRYDTSAI
1460 1470
TQNSNTEGLA TVSKIIKLDR K
Length:1,471
Mass (Da):169,344
Last modified:October 1, 1993 - v1
Checksum:iE79C0FE72B041E95
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M90057 Unassigned DNA. Translation: AAC37409.1.
U12980 Genomic DNA. Translation: AAC05003.1.
BK006935 Genomic DNA. Translation: DAA06959.1.
PIRiS30790.
RefSeqiNP_009373.1. NM_001178174.1.

Genome annotation databases

EnsemblFungiiYAL029C; YAL029C; YAL029C.
GeneIDi851204.
KEGGisce:YAL029C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M90057 Unassigned DNA. Translation: AAC37409.1 .
U12980 Genomic DNA. Translation: AAC05003.1 .
BK006935 Genomic DNA. Translation: DAA06959.1 .
PIRi S30790.
RefSeqi NP_009373.1. NM_001178174.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3MMI X-ray 2.30 A/B 1091-1471 [» ]
4LL6 X-ray 2.30 A 1098-1471 [» ]
4LL8 X-ray 3.58 A 918-1471 [» ]
ProteinModelPortali P32492.
SMRi P32492. Positions 4-840, 1016-1468.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31737. 55 interactions.
DIPi DIP-5761N.
IntActi P32492. 31 interactions.
MINTi MINT-617203.
STRINGi 4932.YAL029C.

Proteomic databases

MaxQBi P32492.
PaxDbi P32492.
PeptideAtlasi P32492.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YAL029C ; YAL029C ; YAL029C .
GeneIDi 851204.
KEGGi sce:YAL029C.

Organism-specific databases

CYGDi YAL029c.
SGDi S000000027. MYO4.

Phylogenomic databases

eggNOGi COG5022.
GeneTreei ENSGT00760000119299.
HOGENOMi HOG000171839.
InParanoidi P32492.
KOi K10357.
OMAi AEENTIV.
OrthoDBi EOG77T1CS.

Enzyme and pathway databases

BioCyci YEAST:G3O-28840-MONOMER.

Miscellaneous databases

EvolutionaryTracei P32492.
NextBioi 968067.

Gene expression databases

Genevestigatori P32492.

Family and domain databases

InterProi IPR018444. Dil_domain.
IPR002710. Dilute.
IPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR008989. Myosin_S1_N.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF01843. DIL. 1 hit.
PF00612. IQ. 1 hit.
PF00063. Myosin_head. 1 hit.
[Graphical view ]
PRINTSi PR00193. MYOSINHEAVY.
SMARTi SM00015. IQ. 4 hits.
SM00242. MYSc. 1 hit.
[Graphical view ]
SUPFAMi SSF50084. SSF50084. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEi PS51126. DILUTE. 1 hit.
PS50096. IQ. 2 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of MYO4, a second class V myosin gene in yeast."
    Haarer B.K., Petzold A., Lillie S.H., Brown S.S.
    J. Cell Sci. 107:1055-1064(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Association of the class V myosin Myo4p with a localised messenger RNA in budding yeast depends on She proteins."
    Munchow S., Sauter C., Jansen R.P.
    J. Cell Sci. 112:1511-1518(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "She2p, a novel RNA-binding protein tethers ASH1 mRNA to the Myo4p myosin motor via She3p."
    Bohl F., Kruse C., Frank A., Ferring D., Jansen R.P.
    EMBO J. 19:5514-5524(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SHE2 AND SHE3.
  6. "She2p is a novel RNA-binding protein that recruits the Myo4p-She3p complex to ASH1 mRNA."
    Long R.M., Gu W., Lorimer E., Singer R.H., Chartrand P.
    EMBO J. 19:6592-6601(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SHE3.
  7. "The myosin motor, Myo4p, binds Ash1 mRNA via the adapter protein, She3p."
    Takizawa P.A., Vale R.D.
    Proc. Natl. Acad. Sci. U.S.A. 97:5273-5278(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SHE2 AND SHE3.
  8. "Ribonucleoprotein-dependent localization of the yeast class V myosin Myo4p."
    Kruse C., Jaedicke A., Beaudouin J., Bohl F., Ferring D., Guttler T., Ellenberg J., Jansen R.P.
    J. Cell Biol. 159:971-982(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "ASH1 mRNA anchoring requires reorganization of the Myo4p-She3p-She2p transport complex."
    Gonsalvez G.B., Little J.L., Long R.M.
    J. Biol. Chem. 279:46286-46294(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "The structure of the Myo4p globular tail and its function in ASH1 mRNA localization."
    Heuck A., Fetka I., Brewer D.N., Huls D., Munson M., Jansen R.P., Niessing D.
    J. Cell Biol. 189:497-510(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 1091-1471, FUNCTION, INTERACTION WITH SHE3.

Entry informationi

Entry nameiMYO4_YEAST
AccessioniPrimary (citable) accession number: P32492
Secondary accession number(s): D6VPI9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 29, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2210 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3