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Protein

Myosin-4

Gene

MYO4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Recruited to specific mRNAs including the ASH1 mRNA, coding for a repressor of the HO endonuclease, via its interaction with SHE3.6 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi165 – 172ATPSequence analysis8

GO - Molecular functioni

  • actin filament binding Source: SGD
  • ATP binding Source: UniProtKB-KW
  • microfilament motor activity Source: SGD

GO - Biological processi

  • endoplasmic reticulum inheritance Source: SGD
  • intracellular mRNA localization Source: SGD
  • mating type switching Source: SGD
  • mRNA transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Myosin

Keywords - Biological processi

mRNA transport, Transport

Keywords - Ligandi

Actin-binding, ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28840-MONOMER.
ReactomeiR-SCE-194840. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-4
Alternative name(s):
SWI5-dependent HO expression protein 1
Gene namesi
Name:MYO4
Synonyms:SHE1
Ordered Locus Names:YAL029C
ORF Names:FUN22
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:YAL029C.
SGDiS000000027. MYO4.

Subcellular locationi

GO - Cellular componenti

  • cellular bud Source: SGD
  • cellular bud tip Source: SGD
  • filamentous actin Source: SGD
  • myosin complex Source: UniProtKB-KW
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001234911 – 1471Myosin-4Add BLAST1471

Proteomic databases

MaxQBiP32492.
PRIDEiP32492.

PTM databases

iPTMnetiP32492.

Interactioni

Subunit structurei

Interacts with SHE2 and SHE3.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SHE2P360684EBI-11681,EBI-26866
SHE3P382727EBI-11681,EBI-21600

GO - Molecular functioni

  • actin filament binding Source: SGD

Protein-protein interaction databases

BioGridi31737. 55 interactors.
DIPiDIP-5761N.
IntActiP32492. 31 interactors.
MINTiMINT-617203.

Structurei

Secondary structure

11471
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1104 – 1109Combined sources6
Helixi1111 – 1121Combined sources11
Helixi1128 – 1130Combined sources3
Helixi1138 – 1152Combined sources15
Helixi1156 – 1176Combined sources21
Turni1179 – 1181Combined sources3
Helixi1182 – 1193Combined sources12
Helixi1196 – 1204Combined sources9
Helixi1205 – 1207Combined sources3
Helixi1217 – 1253Combined sources37
Helixi1255 – 1259Combined sources5
Helixi1262 – 1264Combined sources3
Beta strandi1265 – 1267Combined sources3
Helixi1270 – 1289Combined sources20
Helixi1294 – 1319Combined sources26
Helixi1325 – 1342Combined sources18
Turni1343 – 1345Combined sources3
Helixi1350 – 1353Combined sources4
Helixi1354 – 1364Combined sources11
Helixi1370 – 1377Combined sources8
Helixi1385 – 1393Combined sources9
Beta strandi1398 – 1401Combined sources4
Helixi1407 – 1423Combined sources17
Helixi1440 – 1442Combined sources3
Helixi1460 – 1467Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MMIX-ray2.30A/B1091-1471[»]
4LL6X-ray2.30A1098-1471[»]
4LL8X-ray3.58A918-1471[»]
ProteinModelPortaliP32492.
SMRiP32492.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32492.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini71 – 777Myosin motorAdd BLAST707
Domaini781 – 801IQ 1PROSITE-ProRule annotationAdd BLAST21
Domaini804 – 824IQ 2PROSITE-ProRule annotationAdd BLAST21
Domaini829 – 849IQ 3PROSITE-ProRule annotationAdd BLAST21
Domaini876 – 898IQ 4PROSITE-ProRule annotationAdd BLAST23
Domaini899 – 928IQ 5PROSITE-ProRule annotationAdd BLAST30
Domaini1164 – 1419DilutePROSITE-ProRule annotationAdd BLAST256

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili938 – 1063Add BLAST126

Sequence similaritiesi

Contains 1 dilute domain.PROSITE-ProRule annotation
Contains 5 IQ domains.PROSITE-ProRule annotation
Contains 1 myosin motor domain.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

GeneTreeiENSGT00860000133764.
HOGENOMiHOG000171839.
InParanoidiP32492.
KOiK10357.
OMAiVESIVMS.
OrthoDBiEOG092C04K7.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR002710. Dilute_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR008989. Myosin_S1_N.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01843. DIL. 1 hit.
PF00612. IQ. 1 hit.
PF00063. Myosin_head. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM01132. DIL. 1 hit.
SM00015. IQ. 4 hits.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF50084. SSF50084. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51126. DILUTE. 1 hit.
PS50096. IQ. 2 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32492-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFEVGTKCW YPHKEQGWIG GEVTKNDFFE GTFHLELKLE DGETVSIETN
60 70 80 90 100
SFENDDDHPT LPVLRNPPIL ESTDDLTTLS YLNEPAVLHA IKKRYMNGQI
110 120 130 140 150
YTYSGIVLIA ANPFDKVDHL YSREMIQNYS SKRKDELEPH LFAIAEEAYR
160 170 180 190 200
FMVHEKANQT VVVSGESGAG KTVSAKYIMR YFASVQESNN REGEVEMSQI
210 220 230 240 250
ESQILATNPI MEAFGNAKTT RNDNSSRFGK YLQILFDENT TIRGSKIRTY
260 270 280 290 300
LLEKSRLVYQ PETERNYHIF YQILEGLPEP VKQELHLSSP KDYHYTNQGG
310 320 330 340 350
QPNIAGIDEA REYKITTDAL SLVGINHETQ LGIFKILAGL LHIGNIEMKM
360 370 380 390 400
TRNDASLSSE EQNLQIACEL LGIDPFNFAK WIVKKQIVTR SEKIVTNLNY
410 420 430 440 450
NQALIARDSV AKFIYSTLFD WLVDNINKTL YDPELDQQDH VFSFIGILDI
460 470 480 490 500
YGFEHFEKNS FEQFCINYAN EKLQQEFNQH VFKLEQEEYV KEEIEWSFIE
510 520 530 540 550
FSDNQPCIDL IENKLGILSL LDEESRLPSG SDESWASKLY SAFNKPPSNE
560 570 580 590 600
VFSKPRFGQT KFIVSHYAVD VEYEVEGFIE KNRDSVSLGH LDVFKATTNP
610 620 630 640 650
IFKQILDNRE LRSDDAPEEQ NTEKKIMIPA RLSQKKPTLG SMFKKSLGEL
660 670 680 690 700
MAIINSTNVH YIRCIKPNSE KKPWEFDNLM VLSQLRACGV LETIRISCAG
710 720 730 740 750
FPSRWTFDEF VQRYFLLTDY SLWSGILYNP DLPKEAIVNF CQSILDATIS
760 770 780 790 800
DSAKYQIGNT KIFFKAGMLA FLEKLRTNKM NEICIIIQKK IRARYYRLQY
810 820 830 840 850
LQTMESIKKC QSQIRSLLVR TRVDHELKTR AAILLQTNIR ALWKREYYRA
860 870 880 890 900
AIGQIIKLQC TCKRKLILDS VNRKFMLMAA VIIQSYIRSY GHKTDYRTLK
910 920 930 940 950
RSSILVQSAM RMQLARRRYI VLQKEVEERN IRASYGIGLL EEAIEFKNSF
960 970 980 990 1000
ILNLEMLNDS YTRLTQLLQG DLSNIPSKQR QEYETIVNGY NDKISKLKTL
1010 1020 1030 1040 1050
QVEIMNTLNK KNALKERKKK QSSLIQSHMQ SLAAIKGNKP SRLSDEVKSM
1060 1070 1080 1090 1100
KQELAFIENV IAQDFTTTYS ANKNDKVKGL GIAGQQVKPK LVNVIRRESG
1110 1120 1130 1140 1150
NPDLLELLMD LNCYTLEVTE GYLKKVNVTE VNGDNVLGPI HVITTVVSSL
1160 1170 1180 1190 1200
VRNGLLIQSS KFISKVLLTV ESIVMSLPKD ETMLGGIFWL SNLSRLPAFA
1210 1220 1230 1240 1250
ANQKTLYEAN GGDEKDKLTL IYLNDLENET LKVFDKIYST WLVKFMKHAS
1260 1270 1280 1290 1300
AHIEIFDMVL NEKLFKNSGD EKFAKLFTFL NEFDAVLCKF QVVDSMHTKI
1310 1320 1330 1340 1350
FNDTLKYLNV MLFNDLITKC PALNWKYGYE VDRNIERLVS WFEPRIEDVR
1360 1370 1380 1390 1400
PNLIQIIQAV KILQLKISNL NEFKLLFDFW YALNPAQIQA ILLKYKPANK
1410 1420 1430 1440 1450
GEAGVPNEIL NYLANVIKRE NLSLPGKMEI MLSAQFDSAK NHLRYDTSAI
1460 1470
TQNSNTEGLA TVSKIIKLDR K
Length:1,471
Mass (Da):169,344
Last modified:October 1, 1993 - v1
Checksum:iE79C0FE72B041E95
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90057 Unassigned DNA. Translation: AAC37409.1.
U12980 Genomic DNA. Translation: AAC05003.1.
BK006935 Genomic DNA. Translation: DAA06959.1.
PIRiS30790.
RefSeqiNP_009373.1. NM_001178174.1.

Genome annotation databases

EnsemblFungiiYAL029C; YAL029C; YAL029C.
GeneIDi851204.
KEGGisce:YAL029C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90057 Unassigned DNA. Translation: AAC37409.1.
U12980 Genomic DNA. Translation: AAC05003.1.
BK006935 Genomic DNA. Translation: DAA06959.1.
PIRiS30790.
RefSeqiNP_009373.1. NM_001178174.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MMIX-ray2.30A/B1091-1471[»]
4LL6X-ray2.30A1098-1471[»]
4LL8X-ray3.58A918-1471[»]
ProteinModelPortaliP32492.
SMRiP32492.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31737. 55 interactors.
DIPiDIP-5761N.
IntActiP32492. 31 interactors.
MINTiMINT-617203.

PTM databases

iPTMnetiP32492.

Proteomic databases

MaxQBiP32492.
PRIDEiP32492.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYAL029C; YAL029C; YAL029C.
GeneIDi851204.
KEGGisce:YAL029C.

Organism-specific databases

EuPathDBiFungiDB:YAL029C.
SGDiS000000027. MYO4.

Phylogenomic databases

GeneTreeiENSGT00860000133764.
HOGENOMiHOG000171839.
InParanoidiP32492.
KOiK10357.
OMAiVESIVMS.
OrthoDBiEOG092C04K7.

Enzyme and pathway databases

BioCyciYEAST:G3O-28840-MONOMER.
ReactomeiR-SCE-194840. Rho GTPase cycle.

Miscellaneous databases

EvolutionaryTraceiP32492.
PROiP32492.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR002710. Dilute_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR008989. Myosin_S1_N.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01843. DIL. 1 hit.
PF00612. IQ. 1 hit.
PF00063. Myosin_head. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM01132. DIL. 1 hit.
SM00015. IQ. 4 hits.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF50084. SSF50084. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51126. DILUTE. 1 hit.
PS50096. IQ. 2 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYO4_YEAST
AccessioniPrimary (citable) accession number: P32492
Secondary accession number(s): D6VPI9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 30, 2016
This is version 155 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2210 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.