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Protein

MAP kinase kinase MKK2/SSP33

Gene

MKK2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine protein kinase involved in a signal transduction pathway that plays a role in yeast cell morphogenesis and cell growth. This pathway seems to start by SMP3; then involves the kinase PKC1 that may act on the BCK1 kinase that then phosphorylates MKK1 and MKK2 which themselves phosphorylate the MPK1 kinase.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei243 – 2431ATPPROSITE-ProRule annotation
Active sitei342 – 3421Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi220 – 2289ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • activation of MAPK activity Source: GOC
  • MAPK cascade Source: GOC
  • pexophagy Source: SGD
  • protein phosphorylation Source: SGD
  • regulation of fungal-type cell wall organization Source: SGD
  • signal transduction Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34038-MONOMER.
BRENDAi2.7.12.2. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
MAP kinase kinase MKK2/SSP33 (EC:2.7.12.2)
Gene namesi
Name:MKK2
Synonyms:SSP33
Ordered Locus Names:YPL140C
ORF Names:LPI6C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL140C.
SGDiS000006061. MKK2.

Subcellular locationi

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • cellular bud tip Source: SGD
  • cytoplasm Source: SGD
  • mating projection tip Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 506506MAP kinase kinase MKK2/SSP33PRO_0000086333Add
BLAST

Proteomic databases

MaxQBiP32491.
PeptideAtlasiP32491.

PTM databases

iPTMnetiP32491.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SLT2Q007724EBI-10973,EBI-17372

Protein-protein interaction databases

BioGridi36042. 74 interactions.
DIPiDIP-1447N.
IntActiP32491. 20 interactions.
MINTiMINT-395755.

Structurei

3D structure databases

ProteinModelPortaliP32491.
SMRiP32491. Positions 163-481.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini214 – 481268Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00720000108839.
HOGENOMiHOG000234206.
InParanoidiP32491.
KOiK08294.
OMAiMFRPPES.
OrthoDBiEOG7380F1.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32491-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASMFRPPES NRSHQKTPKL TLPVNLVQNA KSTNDGQHLN RSPYSSVNES
60 70 80 90 100
PYSNNSTSAT STTSSMASNS TLLYNRSSTT TIKNRPVPPP LPPLVLTQKK
110 120 130 140 150
DGIEYRVAGD SQLSERFSNL HVDITYKELL SSAPISTKLS NIDTTFIKKD
160 170 180 190 200
LDTPEGEDSY PSTLLSAYDF SSSGSNSAPL SANNIISCSN LIQGKDVDQL
210 220 230 240 250
EEEAWRFGHL KDEITTLGIL GEGAGGSVAK CRLKNGKKVF ALKTINTMNT
260 270 280 290 300
DPEYQKQIFR ELQFNKSFKS DYIVQYYGMF TDEQSSSIYI AMEYMGGKSL
310 320 330 340 350
EATYKNLLKR GGRISERVIG KIAESVLRGL SYLHERKVIH RDIKPQNILL
360 370 380 390 400
NEKGEIKLCD FGVSGEAVNS LAMTFTGTSF YMAPERIQGQ PYSVTCDVWS
410 420 430 440 450
LGLTLLEVAG GRFPFESDKI TQNVAPIELL TMILTFSPQL KDEPELDISW
460 470 480 490 500
SKTFRSFIDY CLKKDARERP SPRQMLKHPW IVGQMKKKVN MERFVKKCWE

KEKDGI
Length:506
Mass (Da):56,758
Last modified:October 1, 1996 - v2
Checksum:i8EACED6C742E148B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti252 – 2521P → S in BAA02933 (PubMed:8386320).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13785 Genomic DNA. Translation: BAA02933.1.
U43703 Genomic DNA. Translation: AAB68220.1.
U10280 Genomic DNA. Translation: AAB40938.1.
BK006949 Genomic DNA. Translation: DAA11294.1.
PIRiS69045.
RefSeqiNP_015185.1. NM_001183954.1.

Genome annotation databases

EnsemblFungiiYPL140C; YPL140C; YPL140C.
GeneIDi855963.
KEGGisce:YPL140C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13785 Genomic DNA. Translation: BAA02933.1.
U43703 Genomic DNA. Translation: AAB68220.1.
U10280 Genomic DNA. Translation: AAB40938.1.
BK006949 Genomic DNA. Translation: DAA11294.1.
PIRiS69045.
RefSeqiNP_015185.1. NM_001183954.1.

3D structure databases

ProteinModelPortaliP32491.
SMRiP32491. Positions 163-481.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36042. 74 interactions.
DIPiDIP-1447N.
IntActiP32491. 20 interactions.
MINTiMINT-395755.

PTM databases

iPTMnetiP32491.

Proteomic databases

MaxQBiP32491.
PeptideAtlasiP32491.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL140C; YPL140C; YPL140C.
GeneIDi855963.
KEGGisce:YPL140C.

Organism-specific databases

EuPathDBiFungiDB:YPL140C.
SGDiS000006061. MKK2.

Phylogenomic databases

GeneTreeiENSGT00720000108839.
HOGENOMiHOG000234206.
InParanoidiP32491.
KOiK08294.
OMAiMFRPPES.
OrthoDBiEOG7380F1.

Enzyme and pathway databases

BioCyciYEAST:G3O-34038-MONOMER.
BRENDAi2.7.12.2. 984.

Miscellaneous databases

NextBioi980765.
PROiP32491.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "MKK1 and MKK2, which encode Saccharomyces cerevisiae mitogen-activated protein kinase-kinase homologs, function in the pathway mediated by protein kinase C."
    Irie K., Takase M., Lee K.S., Levin D.E., Araki H., Matsumoto K., Oshima Y.
    Mol. Cell. Biol. 13:3076-3083(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Mallory M.J., Strich R.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-88.
    Strain: ATCC 204626 / S288c / A364A.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMKK2_YEAST
AccessioniPrimary (citable) accession number: P32491
Secondary accession number(s): D6W3M8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1950 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.