P32491 (MKK2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
MAP kinase kinase MKK2/SSP33
EC=2.7.12.2

Gene names

Name:	MKK2
Synonyms:	SSP33
Ordered Locus Names:	YPL140C
ORF Names:	LPI6C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Serine/threonine protein kinase involved in a signal transduction pathway that play a role in yeast cell morphogenesis and cell growth. This pathway seems to starts by SMP3; then involves the kinase PKC1 that may act on the BCK1 kinase that then phosphorylates MKK1 and MKK2 which themselves phosphorylate the MPK1 kinase.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Miscellaneous	Present with 1950 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	peroxisome degradation Inferred from genetic interaction. Source: SGD signal transduction Inferred from mutant phenotype Ref.1. Source: SGD
Cellular component	intracellular Traceable author statement. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SLT2	Q00772	5	EBI-10973,EBI-17372

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	MAP kinase kinase MKK2/SSP33		PRO_0000086333
Regions
	Domain	214 – 481	268	Protein kinase
	Nucleotide binding	220 – 228	9	ATP By similarity
Sites
	Active site	342	1	Proton acceptor By similarity
	Binding site	243	1	ATP By similarity
Amino acid modifications
	Modified residue	370	1	Phosphoserine By similarity
	Modified residue	374	1	Phosphothreonine By similarity
Experimental info
	Sequence conflict	252	1	P → S in BAA02933. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32491 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 8EACED6C742E148B	FASTA	506	56,758
	10 20 30 40 50 60 MASMFRPPES NRSHQKTPKL TLPVNLVQNA KSTNDGQHLN RSPYSSVNES PYSNNSTSAT 70 80 90 100 110 120 STTSSMASNS TLLYNRSSTT TIKNRPVPPP LPPLVLTQKK DGIEYRVAGD SQLSERFSNL 130 140 150 160 170 180 HVDITYKELL SSAPISTKLS NIDTTFIKKD LDTPEGEDSY PSTLLSAYDF SSSGSNSAPL 190 200 210 220 230 240 SANNIISCSN LIQGKDVDQL EEEAWRFGHL KDEITTLGIL GEGAGGSVAK CRLKNGKKVF 250 260 270 280 290 300 ALKTINTMNT DPEYQKQIFR ELQFNKSFKS DYIVQYYGMF TDEQSSSIYI AMEYMGGKSL 310 320 330 340 350 360 EATYKNLLKR GGRISERVIG KIAESVLRGL SYLHERKVIH RDIKPQNILL NEKGEIKLCD 370 380 390 400 410 420 FGVSGEAVNS LAMTFTGTSF YMAPERIQGQ PYSVTCDVWS LGLTLLEVAG GRFPFESDKI 430 440 450 460 470 480 TQNVAPIELL TMILTFSPQL KDEPELDISW SKTFRSFIDY CLKKDARERP SPRQMLKHPW 490 500 IVGQMKKKVN MERFVKKCWE KEKDGI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MKK1 and MKK2, which encode Saccharomyces cerevisiae mitogen-activated protein kinase-kinase homologs, function in the pathway mediated by protein kinase C." Irie K., Takase M., Lee K.S., Levin D.E., Araki H., Matsumoto K., Oshima Y. Mol. Cell. Biol. 13:3076-3083(1993) [PubMed: 8386320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	Mallory M.J., Strich R. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-88. Strain: ATCC 204626 / S288c / A364A.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D13785 Genomic DNA. Translation: BAA02933.1. U43703 Genomic DNA. Translation: AAB68220.1. U10280 Genomic DNA. Translation: AAB40938.1. BK006949 Genomic DNA. Translation: DAA11294.1.
PIR	S69045.
RefSeq	NP_015185.1. NM_001183954.1.
3D structure databases
ProteinModelPortal	P32491.
SMR	P32491. Positions 211-497.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1447N.
IntAct	P32491. 29 interactions.
MINT	MINT-395755.
STRING	P32491.
Proteomic databases
PeptideAtlas	P32491.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL140C; YPL140C; YPL140C.
GeneID	855963.
KEGG	sce:YPL140C.
NMPDR	fig|4932.3.peg.6315.
Organism-specific databases
CYGD	YPL140c.
SGD	S000006061. MKK2.
Phylogenomic databases
eggNOG	fuNOG04753.
GeneTree	EFGT00050000000597.
HOGENOM	HBG755340.
OMA	MFRPPES.
OrthoDB	EOG4D29ZP.
Enzyme and pathway databases
BRENDA	2.7.12.2. 984.
Gene expression databases
ArrayExpress	P32491.
Genevestigator	P32491.
GermOnline	YPL140C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K08294.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980765.

Entry information

Entry name

MKK2_YEAST

Accession

Primary (citable) accession number: P32491
Secondary accession number(s): D6W3M8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program