Reviewed, UniProtKB/Swiss-Prot P32490 (MKK1_YEAST)

Names and origin

Protein names

Recommended name:
    MAP kinase kinase MKK1/SSP32
    EC=2.7.12.2

Gene names

Name:	MKK1
Synonyms:	SSP32
Ordered Locus Names:	YOR231W
ORF Names:	O5095

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in a signal transduction pathway that play a role in yeast cell morphogenesis and cell growth. This pathway seems to starts by SMP3; then involve the kinase PKC1 that may act on the BCK1 kinase that then phosphorylates MKK1 and MKK2 which themselves phosphorylate the MPK1 kinase.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Miscellaneous	Present with 1040 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro signal transduction Ref.1 Inferred from mutant phenotype. Source: SGD
Cellular component	cellular bud tip Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 508	508	MAP kinase kinase MKK1/SSP32		PRO_0000086332
Regions
	Domain	221 – 488	268	Protein kinase
	Nucleotide binding	227 – 235	9	ATP By similarity
Sites
	Active site	349	1	Proton acceptor By similarity
	Binding site	250	1	ATP By similarity
Amino acid modifications
	Modified residue	71	1	Phosphoserine Ref.6
	Modified residue	152	1	Phosphoserine Ref.6
	Modified residue	163	1	Phosphoserine Ref.6
	Modified residue	191	1	Phosphoserine Ref.6
	Modified residue	192	1	Phosphoserine Ref.6
	Modified residue	194	1	Phosphoserine Ref.6
	Modified residue	195	1	Phosphotyrosine Ref.6
	Modified residue	377	1	Phosphoserine By similarity
	Modified residue	381	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32490-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 4FE62CDD185CDAA2	FASTA	508	56,720
	10 20 30 40 50 60 MASLFRPPES AKCNPNSPRL KLPLLRNNQV DENNIYLTSN GSSTTAYSSH TPEPLTSSTS 70 80 90 100 110 120 TLFSQTRLHP SDSSMTLNTM KKRPAPPSLP SLSINSQSKC KTLPELVPIA DVSDGKHDLG 130 140 150 160 170 180 LKQRVIAENE LSGNSDLTPS SMASPFSHTN TSSPYLRNDL SNSVGSDFSN LISAYEQSSS 190 200 210 220 230 240 PIKSSSQPKS SSESYIDLNS VRDVDQLDEN GWKYANLKDR IETLGILGEG AGGSVSKCKL 250 260 270 280 290 300 KNGSKIFALK VINTLNTDPE YQKQIFRELQ FNRSFQSEYI VRYYGMFTDD ENSSIYIAME 310 320 330 340 350 360 YMGGRSLDAI YKNLLERGGR ISEKVLGKIA EAVLRGLSYL HEKKVIHRDI KPQNILLNEN 370 380 390 400 410 420 GQVKLCDFGV SGEAVNSLAT TFTGTSFYMA PERIQGQPYS VTSDVWSLGL TILEVANGKF 430 440 450 460 470 480 PCSSEKMAAN IAPFELLMWI LTFTPELKDE PESNIIWSPS FKSFIDYCLK KDSRERPSPR 490 500 QMINHPWIKG QMKKNVNMEK FVRKCWKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MKK1 and MKK2, which encode Saccharomyces cerevisiae mitogen-activated protein kinase-kinase homologs, function in the pathway mediated by protein kinase C." Irie K., Takase M., Lee K.S., Levin D.E., Araki H., Matsumoto K., Oshima Y. Mol. Cell. Biol. 13:3076-3083(1993) [PubMed: 8386320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence and analysis of a 26.9 kb fragment from chromosome XV of the yeast Saccharomyces cerevisiae." Boyer J., Michaux G., Fairhead C., Gaillon L., Dujon B. Yeast 12:1575-1586(1996) [PubMed: 8972580] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE." Zhang Z., Dietrich F.S. Nucleic Acids Res. 33:2838-2851(2005) [PubMed: 15905473] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-74. Strain: ATCC 208353 / W303-1A.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-71; SER-152; SER-163; SER-191; SER-192; SER-194 AND TYR-195, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D13001 Genomic DNA. Translation: BAA02364.1. Z75139 Genomic DNA. Translation: CAA99451.1. AY899252 mRNA. Translation: AAX83937.1.
PIR	A48069.
RefSeq	NP_014874.1.
3D structure databases
HSSP	HSSP built from PDB template 1F3M based on UniProtKB Q13153.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2224N.
IntAct	P32490. 8 interactions.
STRING	P32490.
Genome annotation databases
Ensembl	YOR231W; YOR231W; YOR231W; Saccharomyces cerevisiae. [Genome view]
GeneID	854406.
GenomeReviews	Gene locus YOR231W in contig Y13140_GR.
KEGG	sce:YOR231W.
NMPDR	fig|4932.3.peg.5986.
Organism-specific databases
CYGD	YOR231w.
SGD	S000005757. MKK1.
Phylogenomic databases
HOGENOM	P32490.
OMA	SEYIVRY.
Enzyme and pathway databases
BRENDA	2.7.12.2. 250.
Gene expression databases
ArrayExpress	P32490.
Genevestigator	P32490.
GermOnline	YOR231W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	976589.

Entry information

Entry name

MKK1_YEAST

Accession

Primary (citable) accession number: P32490
Secondary accession number(s): Q2VQW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	November 3, 2009
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)