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P32490 (MKK1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
MAP kinase kinase MKK1/SSP32

EC=2.7.12.2
Gene names
Name:MKK1
Synonyms:SSP32
Ordered Locus Names:YOR231W
ORF Names:O5095
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length508 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in a signal transduction pathway that play a role in yeast cell morphogenesis and cell growth. This pathway seems to start by SMP3; then involve the kinase PKC1 that may act on the BCK1 kinase that then phosphorylates MKK1 and MKK2 which themselves phosphorylate the MPK1 kinase.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Miscellaneous

Present with 1040 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.

Contains 1 protein kinase domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

GIN4Q122632EBI-10968,EBI-7595

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 508508MAP kinase kinase MKK1/SSP32
PRO_0000086332

Regions

Domain221 – 488268Protein kinase
Nucleotide binding227 – 2359ATP By similarity

Sites

Active site3491Proton acceptor By similarity
Binding site2501ATP By similarity

Amino acid modifications

Modified residue1921Phosphoserine Ref.7

Sequences

Sequence LengthMass (Da)Tools
P32490 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 4FE62CDD185CDAA2

FASTA50856,720
        10         20         30         40         50         60 
MASLFRPPES AKCNPNSPRL KLPLLRNNQV DENNIYLTSN GSSTTAYSSH TPEPLTSSTS 

        70         80         90        100        110        120 
TLFSQTRLHP SDSSMTLNTM KKRPAPPSLP SLSINSQSKC KTLPELVPIA DVSDGKHDLG 

       130        140        150        160        170        180 
LKQRVIAENE LSGNSDLTPS SMASPFSHTN TSSPYLRNDL SNSVGSDFSN LISAYEQSSS 

       190        200        210        220        230        240 
PIKSSSQPKS SSESYIDLNS VRDVDQLDEN GWKYANLKDR IETLGILGEG AGGSVSKCKL 

       250        260        270        280        290        300 
KNGSKIFALK VINTLNTDPE YQKQIFRELQ FNRSFQSEYI VRYYGMFTDD ENSSIYIAME 

       310        320        330        340        350        360 
YMGGRSLDAI YKNLLERGGR ISEKVLGKIA EAVLRGLSYL HEKKVIHRDI KPQNILLNEN 

       370        380        390        400        410        420 
GQVKLCDFGV SGEAVNSLAT TFTGTSFYMA PERIQGQPYS VTSDVWSLGL TILEVANGKF 

       430        440        450        460        470        480 
PCSSEKMAAN IAPFELLMWI LTFTPELKDE PESNIIWSPS FKSFIDYCLK KDSRERPSPR 

       490        500 
QMINHPWIKG QMKKNVNMEK FVRKCWKD 

« Hide

References

« Hide 'large scale' references
[1]"MKK1 and MKK2, which encode Saccharomyces cerevisiae mitogen-activated protein kinase-kinase homologs, function in the pathway mediated by protein kinase C."
Irie K., Takase M., Lee K.S., Levin D.E., Araki H., Matsumoto K., Oshima Y.
Mol. Cell. Biol. 13:3076-3083(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence and analysis of a 26.9 kb fragment from chromosome XV of the yeast Saccharomyces cerevisiae."
Boyer J., Michaux G., Fairhead C., Gaillon L., Dujon B.
Yeast 12:1575-1586(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE."
Zhang Z., Dietrich F.S.
Nucleic Acids Res. 33:2838-2851(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-74.
Strain: ATCC 208353 / W303-1A.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-192, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D13001 Genomic DNA. Translation: BAA02364.1.
Z75139 Genomic DNA. Translation: CAA99451.1.
AY899252 mRNA. Translation: AAX83937.1.
BK006948 Genomic DNA. Translation: DAA11001.1.
PIRA48069.
RefSeqNP_014874.1. NM_001183650.1.

3D structure databases

ProteinModelPortalP32490.
SMRP32490. Positions 155-501.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34624. 101 interactions.
DIPDIP-2224N.
IntActP32490. 13 interactions.
MINTMINT-548860.
STRING4932.YOR231W.

Proteomic databases

MaxQBP32490.
PaxDbP32490.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR231W; YOR231W; YOR231W.
GeneID854406.
KEGGsce:YOR231W.

Organism-specific databases

CYGDYOR231w.
SGDS000005757. MKK1.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00690000102311.
HOGENOMHOG000234206.
KOK08294.
OMAPSPRQMI.
OrthoDBEOG7380F1.

Enzyme and pathway databases

BioCycYEAST:G3O-33729-MONOMER.
BRENDA2.7.12.2. 984.

Gene expression databases

GenevestigatorP32490.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio976589.

Entry information

Entry nameMKK1_YEAST
AccessionPrimary (citable) accession number: P32490
Secondary accession number(s): D6W2T5, Q2VQW6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 11, 2014
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families