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Protein

MAP kinase kinase MKK1/SSP32

Gene

MKK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in a signal transduction pathway that play a role in yeast cell morphogenesis and cell growth. This pathway seems to start by SMP3; then involve the kinase PKC1 that may act on the BCK1 kinase that then phosphorylates MKK1 and MKK2 which themselves phosphorylate the MPK1 kinase.

Miscellaneous

Present with 1040 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei250ATPPROSITE-ProRule annotation1
Active sitei349Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi227 – 235ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • pexophagy Source: SGD
  • protein phosphorylation Source: SGD
  • regulation of fungal-type cell wall organization Source: SGD
  • signal transduction Source: SGD

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33729-MONOMER.
BRENDAi2.7.12.2. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
MAP kinase kinase MKK1/SSP32 (EC:2.7.12.2)
Gene namesi
Name:MKK1
Synonyms:SSP32
Ordered Locus Names:YOR231W
ORF Names:O5095
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR231W.
SGDiS000005757. MKK1.

Subcellular locationi

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • cellular bud tip Source: SGD
  • cytoplasm Source: SGD
  • mating projection tip Source: SGD

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000863321 – 508MAP kinase kinase MKK1/SSP32Add BLAST508

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei192PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32490.
PRIDEiP32490.

PTM databases

iPTMnetiP32490.

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34624. 151 interactors.
DIPiDIP-2224N.
IntActiP32490. 15 interactors.
MINTiMINT-548860.

Structurei

3D structure databases

ProteinModelPortaliP32490.
SMRiP32490.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini221 – 488Protein kinasePROSITE-ProRule annotationAdd BLAST268

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00720000108839.
HOGENOMiHOG000234206.
InParanoidiP32490.
KOiK08294.
OMAiANIAPFE.
OrthoDBiEOG092C3RSG.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

P32490-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLFRPPES AKCNPNSPRL KLPLLRNNQV DENNIYLTSN GSSTTAYSSH
60 70 80 90 100
TPEPLTSSTS TLFSQTRLHP SDSSMTLNTM KKRPAPPSLP SLSINSQSKC
110 120 130 140 150
KTLPELVPIA DVSDGKHDLG LKQRVIAENE LSGNSDLTPS SMASPFSHTN
160 170 180 190 200
TSSPYLRNDL SNSVGSDFSN LISAYEQSSS PIKSSSQPKS SSESYIDLNS
210 220 230 240 250
VRDVDQLDEN GWKYANLKDR IETLGILGEG AGGSVSKCKL KNGSKIFALK
260 270 280 290 300
VINTLNTDPE YQKQIFRELQ FNRSFQSEYI VRYYGMFTDD ENSSIYIAME
310 320 330 340 350
YMGGRSLDAI YKNLLERGGR ISEKVLGKIA EAVLRGLSYL HEKKVIHRDI
360 370 380 390 400
KPQNILLNEN GQVKLCDFGV SGEAVNSLAT TFTGTSFYMA PERIQGQPYS
410 420 430 440 450
VTSDVWSLGL TILEVANGKF PCSSEKMAAN IAPFELLMWI LTFTPELKDE
460 470 480 490 500
PESNIIWSPS FKSFIDYCLK KDSRERPSPR QMINHPWIKG QMKKNVNMEK

FVRKCWKD
Length:508
Mass (Da):56,720
Last modified:October 1, 1993 - v1
Checksum:i4FE62CDD185CDAA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13001 Genomic DNA. Translation: BAA02364.1.
Z75139 Genomic DNA. Translation: CAA99451.1.
AY899252 mRNA. Translation: AAX83937.1.
BK006948 Genomic DNA. Translation: DAA11001.1.
PIRiA48069.
RefSeqiNP_014874.1. NM_001183650.1.

Genome annotation databases

EnsemblFungiiYOR231W; YOR231W; YOR231W.
GeneIDi854406.
KEGGisce:YOR231W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13001 Genomic DNA. Translation: BAA02364.1.
Z75139 Genomic DNA. Translation: CAA99451.1.
AY899252 mRNA. Translation: AAX83937.1.
BK006948 Genomic DNA. Translation: DAA11001.1.
PIRiA48069.
RefSeqiNP_014874.1. NM_001183650.1.

3D structure databases

ProteinModelPortaliP32490.
SMRiP32490.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34624. 151 interactors.
DIPiDIP-2224N.
IntActiP32490. 15 interactors.
MINTiMINT-548860.

PTM databases

iPTMnetiP32490.

Proteomic databases

MaxQBiP32490.
PRIDEiP32490.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR231W; YOR231W; YOR231W.
GeneIDi854406.
KEGGisce:YOR231W.

Organism-specific databases

EuPathDBiFungiDB:YOR231W.
SGDiS000005757. MKK1.

Phylogenomic databases

GeneTreeiENSGT00720000108839.
HOGENOMiHOG000234206.
InParanoidiP32490.
KOiK08294.
OMAiANIAPFE.
OrthoDBiEOG092C3RSG.

Enzyme and pathway databases

BioCyciYEAST:G3O-33729-MONOMER.
BRENDAi2.7.12.2. 984.

Miscellaneous databases

PROiPR:P32490.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMKK1_YEAST
AccessioniPrimary (citable) accession number: P32490
Secondary accession number(s): D6W2T5, Q2VQW6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 7, 2017
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.