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Protein

MAP kinase kinase MKK1/SSP32

Gene

MKK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in a signal transduction pathway that play a role in yeast cell morphogenesis and cell growth. This pathway seems to start by SMP3; then involve the kinase PKC1 that may act on the BCK1 kinase that then phosphorylates MKK1 and MKK2 which themselves phosphorylate the MPK1 kinase.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei250 – 2501ATPPROSITE-ProRule annotation
Active sitei349 – 3491Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi227 – 2359ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase kinase activity Source: SGD
  3. protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. activation of MAPK activity Source: GOC
  2. MAPK cascade Source: GOC
  3. peroxisome degradation Source: SGD
  4. protein phosphorylation Source: SGD
  5. regulation of fungal-type cell wall organization Source: SGD
  6. signal transduction Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33729-MONOMER.
BRENDAi2.7.12.2. 984.
ReactomeiREACT_191508. Signaling by FGFR.
REACT_207653. Signal transduction by L1.
REACT_235121. ERK1 activation.
REACT_248688. Signalling to ERK5.
REACT_253962. ERK2 activation.

Names & Taxonomyi

Protein namesi
Recommended name:
MAP kinase kinase MKK1/SSP32 (EC:2.7.12.2)
Gene namesi
Name:MKK1
Synonyms:SSP32
Ordered Locus Names:YOR231W
ORF Names:O5095
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR231w.
SGDiS000005757. MKK1.

Subcellular locationi

GO - Cellular componenti

  1. cellular bud neck Source: SGD
  2. cellular bud tip Source: SGD
  3. cytoplasm Source: SGD
  4. mating projection tip Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 508508MAP kinase kinase MKK1/SSP32PRO_0000086332Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei192 – 1921Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32490.
PaxDbiP32490.

Expressioni

Gene expression databases

GenevestigatoriP32490.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
GIN4Q122632EBI-10968,EBI-7595

Protein-protein interaction databases

BioGridi34624. 102 interactions.
DIPiDIP-2224N.
IntActiP32490. 13 interactions.
MINTiMINT-548860.
STRINGi4932.YOR231W.

Structurei

3D structure databases

ProteinModelPortaliP32490.
SMRiP32490. Positions 155-491.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini221 – 488268Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00690000102311.
HOGENOMiHOG000234206.
InParanoidiP32490.
KOiK08294.
OMAiPSPRQMI.
OrthoDBiEOG7380F1.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32490-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASLFRPPES AKCNPNSPRL KLPLLRNNQV DENNIYLTSN GSSTTAYSSH
60 70 80 90 100
TPEPLTSSTS TLFSQTRLHP SDSSMTLNTM KKRPAPPSLP SLSINSQSKC
110 120 130 140 150
KTLPELVPIA DVSDGKHDLG LKQRVIAENE LSGNSDLTPS SMASPFSHTN
160 170 180 190 200
TSSPYLRNDL SNSVGSDFSN LISAYEQSSS PIKSSSQPKS SSESYIDLNS
210 220 230 240 250
VRDVDQLDEN GWKYANLKDR IETLGILGEG AGGSVSKCKL KNGSKIFALK
260 270 280 290 300
VINTLNTDPE YQKQIFRELQ FNRSFQSEYI VRYYGMFTDD ENSSIYIAME
310 320 330 340 350
YMGGRSLDAI YKNLLERGGR ISEKVLGKIA EAVLRGLSYL HEKKVIHRDI
360 370 380 390 400
KPQNILLNEN GQVKLCDFGV SGEAVNSLAT TFTGTSFYMA PERIQGQPYS
410 420 430 440 450
VTSDVWSLGL TILEVANGKF PCSSEKMAAN IAPFELLMWI LTFTPELKDE
460 470 480 490 500
PESNIIWSPS FKSFIDYCLK KDSRERPSPR QMINHPWIKG QMKKNVNMEK

FVRKCWKD
Length:508
Mass (Da):56,720
Last modified:October 1, 1993 - v1
Checksum:i4FE62CDD185CDAA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13001 Genomic DNA. Translation: BAA02364.1.
Z75139 Genomic DNA. Translation: CAA99451.1.
AY899252 mRNA. Translation: AAX83937.1.
BK006948 Genomic DNA. Translation: DAA11001.1.
PIRiA48069.
RefSeqiNP_014874.1. NM_001183650.1.

Genome annotation databases

EnsemblFungiiYOR231W; YOR231W; YOR231W.
GeneIDi854406.
KEGGisce:YOR231W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13001 Genomic DNA. Translation: BAA02364.1.
Z75139 Genomic DNA. Translation: CAA99451.1.
AY899252 mRNA. Translation: AAX83937.1.
BK006948 Genomic DNA. Translation: DAA11001.1.
PIRiA48069.
RefSeqiNP_014874.1. NM_001183650.1.

3D structure databases

ProteinModelPortaliP32490.
SMRiP32490. Positions 155-491.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34624. 102 interactions.
DIPiDIP-2224N.
IntActiP32490. 13 interactions.
MINTiMINT-548860.
STRINGi4932.YOR231W.

Proteomic databases

MaxQBiP32490.
PaxDbiP32490.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR231W; YOR231W; YOR231W.
GeneIDi854406.
KEGGisce:YOR231W.

Organism-specific databases

CYGDiYOR231w.
SGDiS000005757. MKK1.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00690000102311.
HOGENOMiHOG000234206.
InParanoidiP32490.
KOiK08294.
OMAiPSPRQMI.
OrthoDBiEOG7380F1.

Enzyme and pathway databases

BioCyciYEAST:G3O-33729-MONOMER.
BRENDAi2.7.12.2. 984.
ReactomeiREACT_191508. Signaling by FGFR.
REACT_207653. Signal transduction by L1.
REACT_235121. ERK1 activation.
REACT_248688. Signalling to ERK5.
REACT_253962. ERK2 activation.

Miscellaneous databases

NextBioi976589.

Gene expression databases

GenevestigatoriP32490.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "MKK1 and MKK2, which encode Saccharomyces cerevisiae mitogen-activated protein kinase-kinase homologs, function in the pathway mediated by protein kinase C."
    Irie K., Takase M., Lee K.S., Levin D.E., Araki H., Matsumoto K., Oshima Y.
    Mol. Cell. Biol. 13:3076-3083(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence and analysis of a 26.9 kb fragment from chromosome XV of the yeast Saccharomyces cerevisiae."
    Boyer J., Michaux G., Fairhead C., Gaillon L., Dujon B.
    Yeast 12:1575-1586(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE."
    Zhang Z., Dietrich F.S.
    Nucleic Acids Res. 33:2838-2851(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-74.
    Strain: ATCC 208353 / W303-1A.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-192, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMKK1_YEAST
AccessioniPrimary (citable) accession number: P32490
Secondary accession number(s): D6W2T5, Q2VQW6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: January 7, 2015
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1040 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.