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P32490

- MKK1_YEAST

UniProt

P32490 - MKK1_YEAST

Protein

MAP kinase kinase MKK1/SSP32

Gene

MKK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Involved in a signal transduction pathway that play a role in yeast cell morphogenesis and cell growth. This pathway seems to start by SMP3; then involve the kinase PKC1 that may act on the BCK1 kinase that then phosphorylates MKK1 and MKK2 which themselves phosphorylate the MPK1 kinase.

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei250 – 2501ATPPROSITE-ProRule annotation
    Active sitei349 – 3491Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi227 – 2359ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase kinase activity Source: SGD
    3. protein binding Source: IntAct
    4. protein serine/threonine kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. activation of MAPK activity Source: GOC
    2. MAPK cascade Source: GOC
    3. peroxisome degradation Source: SGD
    4. protein phosphorylation Source: SGD
    5. regulation of fungal-type cell wall organization Source: SGD
    6. signal transduction Source: SGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33729-MONOMER.
    BRENDAi2.7.12.2. 984.
    ReactomeiREACT_191508. Signaling by FGFR.
    REACT_207653. Signal transduction by L1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    MAP kinase kinase MKK1/SSP32 (EC:2.7.12.2)
    Gene namesi
    Name:MKK1
    Synonyms:SSP32
    Ordered Locus Names:YOR231W
    ORF Names:O5095
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR231w.
    SGDiS000005757. MKK1.

    Subcellular locationi

    GO - Cellular componenti

    1. cellular bud neck Source: SGD
    2. cellular bud tip Source: SGD
    3. cytoplasm Source: SGD
    4. mating projection tip Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 508508MAP kinase kinase MKK1/SSP32PRO_0000086332Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei192 – 1921Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP32490.
    PaxDbiP32490.

    Expressioni

    Gene expression databases

    GenevestigatoriP32490.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GIN4Q122632EBI-10968,EBI-7595

    Protein-protein interaction databases

    BioGridi34624. 101 interactions.
    DIPiDIP-2224N.
    IntActiP32490. 13 interactions.
    MINTiMINT-548860.
    STRINGi4932.YOR231W.

    Structurei

    3D structure databases

    ProteinModelPortaliP32490.
    SMRiP32490. Positions 155-501.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini221 – 488268Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00690000102311.
    HOGENOMiHOG000234206.
    KOiK08294.
    OMAiPSPRQMI.
    OrthoDBiEOG7380F1.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32490-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASLFRPPES AKCNPNSPRL KLPLLRNNQV DENNIYLTSN GSSTTAYSSH    50
    TPEPLTSSTS TLFSQTRLHP SDSSMTLNTM KKRPAPPSLP SLSINSQSKC 100
    KTLPELVPIA DVSDGKHDLG LKQRVIAENE LSGNSDLTPS SMASPFSHTN 150
    TSSPYLRNDL SNSVGSDFSN LISAYEQSSS PIKSSSQPKS SSESYIDLNS 200
    VRDVDQLDEN GWKYANLKDR IETLGILGEG AGGSVSKCKL KNGSKIFALK 250
    VINTLNTDPE YQKQIFRELQ FNRSFQSEYI VRYYGMFTDD ENSSIYIAME 300
    YMGGRSLDAI YKNLLERGGR ISEKVLGKIA EAVLRGLSYL HEKKVIHRDI 350
    KPQNILLNEN GQVKLCDFGV SGEAVNSLAT TFTGTSFYMA PERIQGQPYS 400
    VTSDVWSLGL TILEVANGKF PCSSEKMAAN IAPFELLMWI LTFTPELKDE 450
    PESNIIWSPS FKSFIDYCLK KDSRERPSPR QMINHPWIKG QMKKNVNMEK 500
    FVRKCWKD 508
    Length:508
    Mass (Da):56,720
    Last modified:October 1, 1993 - v1
    Checksum:i4FE62CDD185CDAA2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13001 Genomic DNA. Translation: BAA02364.1.
    Z75139 Genomic DNA. Translation: CAA99451.1.
    AY899252 mRNA. Translation: AAX83937.1.
    BK006948 Genomic DNA. Translation: DAA11001.1.
    PIRiA48069.
    RefSeqiNP_014874.1. NM_001183650.1.

    Genome annotation databases

    EnsemblFungiiYOR231W; YOR231W; YOR231W.
    GeneIDi854406.
    KEGGisce:YOR231W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13001 Genomic DNA. Translation: BAA02364.1 .
    Z75139 Genomic DNA. Translation: CAA99451.1 .
    AY899252 mRNA. Translation: AAX83937.1 .
    BK006948 Genomic DNA. Translation: DAA11001.1 .
    PIRi A48069.
    RefSeqi NP_014874.1. NM_001183650.1.

    3D structure databases

    ProteinModelPortali P32490.
    SMRi P32490. Positions 155-501.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34624. 101 interactions.
    DIPi DIP-2224N.
    IntActi P32490. 13 interactions.
    MINTi MINT-548860.
    STRINGi 4932.YOR231W.

    Proteomic databases

    MaxQBi P32490.
    PaxDbi P32490.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR231W ; YOR231W ; YOR231W .
    GeneIDi 854406.
    KEGGi sce:YOR231W.

    Organism-specific databases

    CYGDi YOR231w.
    SGDi S000005757. MKK1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00690000102311.
    HOGENOMi HOG000234206.
    KOi K08294.
    OMAi PSPRQMI.
    OrthoDBi EOG7380F1.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33729-MONOMER.
    BRENDAi 2.7.12.2. 984.
    Reactomei REACT_191508. Signaling by FGFR.
    REACT_207653. Signal transduction by L1.

    Miscellaneous databases

    NextBioi 976589.

    Gene expression databases

    Genevestigatori P32490.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "MKK1 and MKK2, which encode Saccharomyces cerevisiae mitogen-activated protein kinase-kinase homologs, function in the pathway mediated by protein kinase C."
      Irie K., Takase M., Lee K.S., Levin D.E., Araki H., Matsumoto K., Oshima Y.
      Mol. Cell. Biol. 13:3076-3083(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequence and analysis of a 26.9 kb fragment from chromosome XV of the yeast Saccharomyces cerevisiae."
      Boyer J., Michaux G., Fairhead C., Gaillon L., Dujon B.
      Yeast 12:1575-1586(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE."
      Zhang Z., Dietrich F.S.
      Nucleic Acids Res. 33:2838-2851(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-74.
      Strain: ATCC 208353 / W303-1A.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-192, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMKK1_YEAST
    AccessioniPrimary (citable) accession number: P32490
    Secondary accession number(s): D6W2T5, Q2VQW6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 136 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1040 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3