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P32487 (LYP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysine-specific permease
Gene names
Name:LYP1
Ordered Locus Names:YNL268W
ORF Names:N0790
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length611 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

High-affinity permease for lysine. Ref.5

Subcellular location

Membrane; Multi-pass membrane protein.

Miscellaneous

Present with 2580 molecules/cell in log phase SD medium. Ref.6

Sequence similarities

Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family. [View classification]

Ontologies

Keywords
   Biological processAmino-acid transport
Transport
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

mitochondrion

Inferred from direct assay. Source: SGD

   Molecular functionbasic amino acid transmembrane transporter activity

Inferred from direct assay Ref.5. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 611611Lysine-specific permease
PRO_0000054156

Regions

Topological domain1 – 115115Cytoplasmic Potential
Transmembrane116 – 13621Helical; Potential
Topological domain137 – 1415Extracellular Potential
Transmembrane142 – 16221Helical; Potential
Topological domain163 – 18523Cytoplasmic Potential
Transmembrane186 – 20520Helical; Potential
Topological domain206 – 22318Extracellular Potential
Transmembrane224 – 24320Helical; Potential
Topological domain244 – 25613Cytoplasmic Potential
Transmembrane257 – 27418Helical; Potential
Topological domain275 – 30329Extracellular Potential
Transmembrane304 – 32118Helical; Potential
Topological domain322 – 34423Cytoplasmic Potential
Transmembrane345 – 36521Helical; Potential
Topological domain366 – 39833Extracellular Potential
Transmembrane399 – 41820Helical; Potential
Topological domain419 – 44527Cytoplasmic Potential
Transmembrane446 – 46419Helical; Potential
Topological domain465 – 4739Extracellular Potential
Transmembrane474 – 49421Helical; Potential
Topological domain495 – 51319Cytoplasmic Potential
Transmembrane514 – 53219Helical; Potential
Topological domain533 – 54715Extracellular Potential
Transmembrane548 – 56619Helical; Potential
Topological domain567 – 61145Cytoplasmic Potential

Amino acid modifications

Modified residue751Phosphoserine Ref.8
Modified residue791Phosphoserine Ref.8
Modified residue871Phosphoserine Ref.10
Modified residue901Phosphothreonine Ref.7 Ref.10

Experimental info

Sequence conflict901T → P in CAA47729. Ref.1
Sequence conflict931N → D in CAA47729. Ref.1
Sequence conflict5611V → M in CAA47729. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P32487 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 4E7AF6F7F5F1461B

FASTA61168,090
        10         20         30         40         50         60 
MGRFSNIITS NKWDEKQNNI GEQSMQELPE DQIEHEMEAI DPSNKTTPYS IDEKQYNTKK 

        70         80         90        100        110        120 
KHGSLQGGAI ADVNSITNSL TRLQVVSHET DINEDEEEAH YEDKHVKRAL KQRHIGMIAL 

       130        140        150        160        170        180 
GGTIGTGLFV GISTPLSNAG PVGSLIAYIF MGTIVYFVTQ SLGEMATFIP VTSSITVFSK 

       190        200        210        220        230        240 
RFLSPAFGVS NGYMYWFNWA ITYAVEVSVI GQVIEYWTDK VPLAAWIAIF WVIITLMNFF 

       250        260        270        280        290        300 
PVKVYGEFEF WVASVKVLAI MGYLIYALII VCGGSHQGPI GFRYWRNPGA WGPGIISSDK 

       310        320        330        340        350        360 
SEGRFLGWVS SLINAAFTYQ GTELVGITAG EAANPRKTVP RAINKVVFRI VLFYIMSLFF 

       370        380        390        400        410        420 
IGLLVPYNDS RLSASSAVIA SSPFVISIQN AGTYALPDIF NAVVLITVVS AANSNVYVGS 

       430        440        450        460        470        480 
RVLYSLARTG NAPKQFGYVT RQGVPYLGVV CTAALGLLAF LVVNNNANTA FNWLINISTL 

       490        500        510        520        530        540 
AGLCAWLFIS LAHIRFMQAL KHRGISRDDL PFKAKLMPYG AYYAAFFVTV IIFIQGFQAF 

       550        560        570        580        590        600 
CPFKVSEFFT SYISLILLAV VFIGCQIYYK CRFIWKLEDI DIDSDRREIE AIIWEDDEPK 

       610 
NLWEKFWAAV A

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequencing of the Saccharomyces cerevisiae gene LYP1 coding for a lysine-specific permease."
Sychrova H., Chevallier M.R.
Yeast 9:771-782(1993) [PubMed: 8368011] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]"The sequence of a 24,152 bp segment from the left arm of chromosome XIV from Saccharomyces cerevisiae between the BNI1 and the POL2 genes."
Sen-Gupta M., Lyck R., Fleig U., Niedenthal R.K., Hegemann J.H.
Yeast 12:505-514(1996) [PubMed: 8740425] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed: 9169873] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Substrate specificity and gene expression of the amino-acid permeases in Saccharomyces cerevisiae."
Regenberg B., During-Olsen L., Kielland-Brandt M.C., Holmberg S.
Curr. Genet. 36:317-328(1999) [PubMed: 10654085] [Abstract]
Cited for: FUNCTION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"A proteomics approach to understanding protein ubiquitination."
Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P.
Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-90, MASS SPECTROMETRY.
Strain: SUB592.
[8]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-75 AND SER-79, MASS SPECTROMETRY.
Strain: YAL6B.
[9]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87 AND THR-90, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X67315 Genomic DNA. Translation: CAA47729.1.
X92494 Genomic DNA. Translation: CAA63230.1.
Z71544 Genomic DNA. Translation: CAA96175.1.
BK006947 Genomic DNA. Translation: DAA10292.1.
PIRS60914.
RefSeqNP_014131.1. NM_001183106.1.

3D structure databases

ProteinModelPortalP32487.
ModBaseSearch...

Protein-protein interaction databases

IntActP32487. 5 interactions.
MINTMINT-2784512.
STRINGP32487.

Protein family/group databases

TCDB2.A.3.10.10. amino acid-polyamine-organocation (APC) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL268W; YNL268W; YNL268W.
GeneID855453.
KEGGsce:YNL268W.
NMPDRfig|4932.3.peg.5196.

Organism-specific databases

CYGDYNL268w.
SGDS000005212. LYP1.

Phylogenomic databases

eggNOGfuNOG04851.
GeneTreeEFGT00050000001131.
HOGENOMHBG492579.
OMALAHIRFM.
OrthoDBEOG4JDMG2.

Gene expression databases

ArrayExpressP32487.
GenevestigatorP32487.
GermOnlineYNL268W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR004841. AA-permease_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERPTHR11785. AA/rel_permease1. 1 hit.
PfamPF00324. AA_permease. 1 hit.
[Graphical view]
PIRSFPIRSF006060. AA_transporter. 1 hit.
TIGRFAMsTIGR00913. 2A0310. 1 hit.
PROSITEPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979367.

Entry information

Entry nameLYP1_YEAST
AccessionPrimary (citable) accession number: P32487
Secondary accession number(s): D6W0S6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: December 14, 2011
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents