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Protein

Lysine-specific permease

Gene

LYP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity permease for lysine.1 Publication

GO - Molecular functioni

  • basic amino acid transmembrane transporter activity Source: SGD

GO - Biological processi

  • basic amino acid transmembrane transport Source: GOC
  • basic amino acid transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33262-MONOMER.

Protein family/group databases

TCDBi2.A.3.10.10. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysine-specific permease
Gene namesi
Name:LYP1
Ordered Locus Names:YNL268W
ORF Names:N0790
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL268W.
SGDiS000005212. LYP1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 115115CytoplasmicSequence analysisAdd
BLAST
Transmembranei116 – 13621HelicalSequence analysisAdd
BLAST
Topological domaini137 – 1415ExtracellularSequence analysis
Transmembranei142 – 16221HelicalSequence analysisAdd
BLAST
Topological domaini163 – 18523CytoplasmicSequence analysisAdd
BLAST
Transmembranei186 – 20520HelicalSequence analysisAdd
BLAST
Topological domaini206 – 22318ExtracellularSequence analysisAdd
BLAST
Transmembranei224 – 24320HelicalSequence analysisAdd
BLAST
Topological domaini244 – 25613CytoplasmicSequence analysisAdd
BLAST
Transmembranei257 – 27418HelicalSequence analysisAdd
BLAST
Topological domaini275 – 30329ExtracellularSequence analysisAdd
BLAST
Transmembranei304 – 32118HelicalSequence analysisAdd
BLAST
Topological domaini322 – 34423CytoplasmicSequence analysisAdd
BLAST
Transmembranei345 – 36521HelicalSequence analysisAdd
BLAST
Topological domaini366 – 39833ExtracellularSequence analysisAdd
BLAST
Transmembranei399 – 41820HelicalSequence analysisAdd
BLAST
Topological domaini419 – 44527CytoplasmicSequence analysisAdd
BLAST
Transmembranei446 – 46419HelicalSequence analysisAdd
BLAST
Topological domaini465 – 4739ExtracellularSequence analysis
Transmembranei474 – 49421HelicalSequence analysisAdd
BLAST
Topological domaini495 – 51319CytoplasmicSequence analysisAdd
BLAST
Transmembranei514 – 53219HelicalSequence analysisAdd
BLAST
Topological domaini533 – 54715ExtracellularSequence analysisAdd
BLAST
Transmembranei548 – 56619HelicalSequence analysisAdd
BLAST
Topological domaini567 – 61145CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 611611Lysine-specific permeasePRO_0000054156Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei64 – 641PhosphoserineCombined sources
Modified residuei75 – 751PhosphoserineCombined sources
Modified residuei77 – 771PhosphothreonineCombined sources
Modified residuei79 – 791PhosphoserineCombined sources
Modified residuei87 – 871PhosphoserineCombined sources
Modified residuei90 – 901PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32487.

PTM databases

iPTMnetiP32487.

Interactioni

Protein-protein interaction databases

BioGridi35572. 28 interactions.
IntActiP32487. 4 interactions.
MINTiMINT-2784512.

Structurei

3D structure databases

ProteinModelPortaliP32487.
SMRiP32487. Positions 115-562.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000049744.
HOGENOMiHOG000261850.
InParanoidiP32487.
KOiK16261.
OMAiQGLKPRH.
OrthoDBiEOG74J9HZ.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 2 hits.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00913. 2A0310. 1 hit.
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32487-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRFSNIITS NKWDEKQNNI GEQSMQELPE DQIEHEMEAI DPSNKTTPYS
60 70 80 90 100
IDEKQYNTKK KHGSLQGGAI ADVNSITNSL TRLQVVSHET DINEDEEEAH
110 120 130 140 150
YEDKHVKRAL KQRHIGMIAL GGTIGTGLFV GISTPLSNAG PVGSLIAYIF
160 170 180 190 200
MGTIVYFVTQ SLGEMATFIP VTSSITVFSK RFLSPAFGVS NGYMYWFNWA
210 220 230 240 250
ITYAVEVSVI GQVIEYWTDK VPLAAWIAIF WVIITLMNFF PVKVYGEFEF
260 270 280 290 300
WVASVKVLAI MGYLIYALII VCGGSHQGPI GFRYWRNPGA WGPGIISSDK
310 320 330 340 350
SEGRFLGWVS SLINAAFTYQ GTELVGITAG EAANPRKTVP RAINKVVFRI
360 370 380 390 400
VLFYIMSLFF IGLLVPYNDS RLSASSAVIA SSPFVISIQN AGTYALPDIF
410 420 430 440 450
NAVVLITVVS AANSNVYVGS RVLYSLARTG NAPKQFGYVT RQGVPYLGVV
460 470 480 490 500
CTAALGLLAF LVVNNNANTA FNWLINISTL AGLCAWLFIS LAHIRFMQAL
510 520 530 540 550
KHRGISRDDL PFKAKLMPYG AYYAAFFVTV IIFIQGFQAF CPFKVSEFFT
560 570 580 590 600
SYISLILLAV VFIGCQIYYK CRFIWKLEDI DIDSDRREIE AIIWEDDEPK
610
NLWEKFWAAV A
Length:611
Mass (Da):68,090
Last modified:October 1, 1996 - v2
Checksum:i4E7AF6F7F5F1461B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti90 – 901T → P in CAA47729 (PubMed:8368011).Curated
Sequence conflicti93 – 931N → D in CAA47729 (PubMed:8368011).Curated
Sequence conflicti561 – 5611V → M in CAA47729 (PubMed:8368011).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67315 Genomic DNA. Translation: CAA47729.1.
X92494 Genomic DNA. Translation: CAA63230.1.
Z71544 Genomic DNA. Translation: CAA96175.1.
BK006947 Genomic DNA. Translation: DAA10292.1.
PIRiS60914.
RefSeqiNP_014131.1. NM_001183106.1.

Genome annotation databases

EnsemblFungiiYNL268W; YNL268W; YNL268W.
GeneIDi855453.
KEGGisce:YNL268W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67315 Genomic DNA. Translation: CAA47729.1.
X92494 Genomic DNA. Translation: CAA63230.1.
Z71544 Genomic DNA. Translation: CAA96175.1.
BK006947 Genomic DNA. Translation: DAA10292.1.
PIRiS60914.
RefSeqiNP_014131.1. NM_001183106.1.

3D structure databases

ProteinModelPortaliP32487.
SMRiP32487. Positions 115-562.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35572. 28 interactions.
IntActiP32487. 4 interactions.
MINTiMINT-2784512.

Protein family/group databases

TCDBi2.A.3.10.10. the amino acid-polyamine-organocation (apc) family.

PTM databases

iPTMnetiP32487.

Proteomic databases

MaxQBiP32487.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL268W; YNL268W; YNL268W.
GeneIDi855453.
KEGGisce:YNL268W.

Organism-specific databases

EuPathDBiFungiDB:YNL268W.
SGDiS000005212. LYP1.

Phylogenomic databases

GeneTreeiENSGT00510000049744.
HOGENOMiHOG000261850.
InParanoidiP32487.
KOiK16261.
OMAiQGLKPRH.
OrthoDBiEOG74J9HZ.

Enzyme and pathway databases

BioCyciYEAST:G3O-33262-MONOMER.

Miscellaneous databases

NextBioi979367.
PROiP32487.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 2 hits.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00913. 2A0310. 1 hit.
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of the Saccharomyces cerevisiae gene LYP1 coding for a lysine-specific permease."
    Sychrova H., Chevallier M.R.
    Yeast 9:771-782(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 28383 / FL100 / VTT C-80102.
  2. "The sequence of a 24,152 bp segment from the left arm of chromosome XIV from Saccharomyces cerevisiae between the BNI1 and the POL2 genes."
    Sen-Gupta M., Lyck R., Fleig U., Niedenthal R.K., Hegemann J.H.
    Yeast 12:505-514(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Substrate specificity and gene expression of the amino-acid permeases in Saccharomyces cerevisiae."
    Regenberg B., During-Olsen L., Kielland-Brandt M.C., Holmberg S.
    Curr. Genet. 36:317-328(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  8. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87 AND THR-90, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-64; SER-75; THR-77; SER-79; SER-87 AND THR-90, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLYP1_YEAST
AccessioniPrimary (citable) accession number: P32487
Secondary accession number(s): D6W0S6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2580 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.