Lysine-specific permease - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P32487 (LYP1_YEAST)

Last modified November 24, 2009. Version 89. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Lysine-specific permease

Gene names

Name:	LYP1
Ordered Locus Names:	YNL268W
ORF Names:	N0790

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	611 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	High-affinity permease for lysine. Ref.4
Subcellular location	Membrane; Multi-pass membrane protein.
Miscellaneous	Present with 2580 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family. [View classification]

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Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Membrane
Domain	Transmembrane
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	basic amino acid transport Ref.4 Inferred from direct assay. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay. Source: SGD
Molecular function	basic amino acid transmembrane transporter activity Ref.4 Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
DIP5	P53388	1	EBI-10258,EBI-5904
GAP1	P19145	1	EBI-10258,EBI-7314
MUP1	P50276	1	EBI-10258,EBI-11624

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 611

611

Lysine-specific permease

PRO_0000054156

Regions

Topological domain

1 – 115

115

Cytoplasmic Potential

Transmembrane

116 – 136

Potential

Topological domain

137 – 141

Extracellular Potential

Transmembrane

142 – 162

Potential

Topological domain

163 – 185

Cytoplasmic Potential

Transmembrane

186 – 205

Potential

Topological domain

206 – 223

Extracellular Potential

Transmembrane

224 – 243

Potential

Topological domain

244 – 256

Cytoplasmic Potential

Transmembrane

257 – 274

Potential

Topological domain

275 – 303

Extracellular Potential

Transmembrane

304 – 321

Potential

Topological domain

322 – 344

Cytoplasmic Potential

Transmembrane

345 – 365

Potential

Topological domain

366 – 398

Extracellular Potential

Transmembrane

399 – 418

Potential

Topological domain

419 – 445

Cytoplasmic Potential

Transmembrane

446 – 464

Potential

Topological domain

465 – 473

Extracellular Potential

Transmembrane

474 – 494

Potential

Topological domain

495 – 513

Cytoplasmic Potential

Transmembrane

514 – 532

Potential

Topological domain

533 – 547

Extracellular Potential

Transmembrane

548 – 566

Potential

Topological domain

567 – 611

Cytoplasmic Potential

Amino acid modifications

Modified residue

Phosphoserine Ref.7

Modified residue

Phosphoserine Ref.7

Modified residue

Phosphoserine Ref.9

Modified residue

Phosphothreonine Ref.9 Ref.6

Experimental info

Sequence conflict

T → P in CAA47729. Ref.1

Sequence conflict

N → D in CAA47729. Ref.1

Sequence conflict

561

V → M in CAA47729. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P32487-1 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 4E7AF6F7F5F1461B
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FASTA

611

68,090

        10         20         30         40         50         60 
MGRFSNIITS NKWDEKQNNI GEQSMQELPE DQIEHEMEAI DPSNKTTPYS IDEKQYNTKK 

        70         80         90        100        110        120 
KHGSLQGGAI ADVNSITNSL TRLQVVSHET DINEDEEEAH YEDKHVKRAL KQRHIGMIAL 

       130        140        150        160        170        180 
GGTIGTGLFV GISTPLSNAG PVGSLIAYIF MGTIVYFVTQ SLGEMATFIP VTSSITVFSK 

       190        200        210        220        230        240 
RFLSPAFGVS NGYMYWFNWA ITYAVEVSVI GQVIEYWTDK VPLAAWIAIF WVIITLMNFF 

       250        260        270        280        290        300 
PVKVYGEFEF WVASVKVLAI MGYLIYALII VCGGSHQGPI GFRYWRNPGA WGPGIISSDK 

       310        320        330        340        350        360 
SEGRFLGWVS SLINAAFTYQ GTELVGITAG EAANPRKTVP RAINKVVFRI VLFYIMSLFF 

       370        380        390        400        410        420 
IGLLVPYNDS RLSASSAVIA SSPFVISIQN AGTYALPDIF NAVVLITVVS AANSNVYVGS 

       430        440        450        460        470        480 
RVLYSLARTG NAPKQFGYVT RQGVPYLGVV CTAALGLLAF LVVNNNANTA FNWLINISTL 

       490        500        510        520        530        540 
AGLCAWLFIS LAHIRFMQAL KHRGISRDDL PFKAKLMPYG AYYAAFFVTV IIFIQGFQAF 

       550        560        570        580        590        600 
CPFKVSEFFT SYISLILLAV VFIGCQIYYK CRFIWKLEDI DIDSDRREIE AIIWEDDEPK 

       610 
NLWEKFWAAV A

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of the Saccharomyces cerevisiae gene LYP1 coding for a lysine-specific permease." Sychrova H., Chevallier M.R. Yeast 9:771-782(1993) [PubMed: 8368011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]	"The sequence of a 24,152 bp segment from the left arm of chromosome XIV from Saccharomyces cerevisiae between the BNI1 and the POL2 genes." Sen-Gupta M., Lyck R., Fleig U., Niedenthal R.K., Hegemann J.H. Yeast 12:505-514(1996) [PubMed: 8740425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Substrate specificity and gene expression of the amino-acid permeases in Saccharomyces cerevisiae." Regenberg B., During-Olsen L., Kielland-Brandt M.C., Holmberg S. Curr. Genet. 36:317-328(1999) [PubMed: 10654085] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-90, MASS SPECTROMETRY.
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-75 AND SER-79, MASS SPECTROMETRY.
[8]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87 AND THR-90, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	X67315 Genomic DNA. Translation: CAA47729.1. X92494 Genomic DNA. Translation: CAA63230.1. Z71544 Genomic DNA. Translation: CAA96175.1.
PIR	S60914.
RefSeq	NP_014131.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P32487. 8 interactions.
STRING	P32487.
Protein family/group databases
TCDB	2.A.3.10.10. amino acid-polyamine-organocation (APC) family.
Genome annotation databases
Ensembl	YNL268W; YNL268W; YNL268W; Saccharomyces cerevisiae. [Genome view]
GeneID	855453.
KEGG	sce:YNL268W.
NMPDR	fig\|4932.3.peg.5196.
Organism-specific databases
CYGD	YNL268w.
SGD	S000005212. LYP1.
Phylogenomic databases
HOGENOM	P32487.
OMA	AHIRFMQ
OrthoDB	EOG99W3XF
Gene expression databases
ArrayExpress	P32487.
Genevestigator	P32487.
GermOnline	YNL268W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002293. AA/rel_permease1. IPR004762. Amino_acid_permease_fungi. IPR004840. Amoino_acid_permease_CS. IPR004841. Permease_region. [Graphical view]
PANTHER	PTHR11785. AA/rel_permease1. 1 hit.
Pfam	PF00324. AA_permease. 1 hit. [Graphical view]
PIRSF	PIRSF006060. AA_transporter. 1 hit.
TIGRFAMs	TIGR00913. 2A0310. 1 hit.
PROSITE	PS00218. AMINO_ACID_PERMEASE_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	979367.

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Entry information

Entry name

LYP1_YEAST

Accession

Primary (citable) accession number: P32487

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1996
Last modified:	November 24, 2009
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents