Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P32486

- KRE6_YEAST

UniProt

P32486 - KRE6_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Beta-glucan synthesis-associated protein KRE6

Gene

KRE6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the synthesis of (1->6)- and (1->3)-beta-D-glucan polymers of the yeast cell wall in vivo. It is required for full activity of beta-glucan synthase in vitro. May be involved in the maturation and transport of cell wall proteins (CWP) to the cell wall. May act as a transglucosidase and contribute to the construction of a protein-bound glucan-structure that acts as an acceptor site for the addition of (1->6)-beta-D-glucan at the cell surface.3 Publications

GO - Molecular functioni

  1. glucosidase activity Source: SGD

GO - Biological processi

  1. (1->6)-beta-D-glucan biosynthetic process Source: SGD
  2. fungal-type cell wall organization Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:YPR159W-MONOMER.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucan synthesis-associated protein KRE6
Alternative name(s):
Killer toxin-resistance protein 6
Gene namesi
Name:KRE6
Synonyms:CWH48
Ordered Locus Names:YPR159W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPR159w.
SGDiS000006363. KRE6.

Subcellular locationi

Golgi apparatus membrane 1 Publication; Single-pass type II membrane protein 1 Publication

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB-KW
  2. integral component of endoplasmic reticulum membrane Source: SGD
  3. integral component of membrane Source: SGD
  4. plasma membrane Source: SGD
  5. site of polarized growth Source: SGD
  6. transport vesicle Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 720720Beta-glucan synthesis-associated protein KRE6PRO_0000084331Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei81 – 811Phosphoserine1 Publication
Modified residuei116 – 1161Phosphoserine1 Publication
Modified residuei133 – 1331Phosphoserine1 Publication
Modified residuei134 – 1341Phosphoserine2 Publications
Modified residuei136 – 1361Phosphoserine1 Publication
Modified residuei139 – 1391Phosphoserine1 Publication
Glycosylationi374 – 3741N-linked (GlcNAc...)Sequence Analysis
Glycosylationi461 – 4611N-linked (GlcNAc...)Sequence Analysis
Glycosylationi538 – 5381N-linked (GlcNAc...)Sequence Analysis
Glycosylationi563 – 5631N-linked (GlcNAc...)Sequence Analysis
Glycosylationi691 – 6911N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP32486.
PaxDbiP32486.
PeptideAtlasiP32486.

Expressioni

Gene expression databases

GenevestigatoriP32486.

Interactioni

Subunit structurei

The cytoplasmic domain interacts with the actin patch assembly proteins LAS17 and SLA1. Interacts with KEG1.2 Publications

Protein-protein interaction databases

BioGridi36331. 71 interactions.
DIPiDIP-1850N.
IntActiP32486. 10 interactions.
MINTiMINT-720927.
STRINGi4932.YPR159W.

Structurei

3D structure databases

ProteinModelPortaliP32486.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 252252CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini274 – 720447LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei253 – 27321Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the SKN1/KRE6 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2273.
GeneTreeiENSGT00390000008087.
HOGENOMiHOG000164283.
InParanoidiP32486.
OMAiKVCFTQG.
OrthoDBiEOG7S4XFR.

Family and domain databases

Gene3Di2.60.120.200. 3 hits.
InterProiIPR013320. ConA-like_dom.
IPR005629. SKN1.
[Graphical view]
PfamiPF03935. SKN1. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.

Sequencei

Sequence statusi: Complete.

P32486-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPLRNLTETH NFSSTNLDTD GTGDDHDGAP LSSSPSFGQQ NDNSTNDNAG
60 70 80 90 100
LTNPFMGSDE ESNARDGESL SSSVHYQPQG SDSSLLHDNS RLDLSQNKGV
110 120 130 140 150
SDYKGYYSRN NSRAVSTAND NSFLQPPHRA IASSPSLNSN LSKNDILSPP
160 170 180 190 200
EFDRYPLVGS RVTSMTQLNH HGRSPTSSPG NESSASFSSN PFLGEQDFSP
210 220 230 240 250
FGGYPASSFP LMIDEKEEDD YLHNPDPEEE ARLDRRRFID DFKYMDKRSA
260 270 280 290 300
SGLAGVLLLF LAAIFIFIVL PALTFTGAID HESNTEEVTY LTQYQYPQLS
310 320 330 340 350
AIRTSLVDPD TPDTAKTREA MDGSKWELVF SDEFNAEGRT FYDGDDPYWT
360 370 380 390 400
APDVHYDATK DLEWYSPDAS TTVNGTLQLR MDAFKNHGLY YRSGMLQSWN
410 420 430 440 450
KVCFTQGALE ISANLPNYGR VSGLWPGLWT MGNLGRPGYL ASTQGVWPYS
460 470 480 490 500
YESCDAGITP NQSSPDGISY LPGQKLSICT CDGEDHPNQG VGRGAPEIDV
510 520 530 540 550
LEGETDTKIG VGIASQSLQI APFDIWYMPD YDFIEVYNFT TTTMNTYAGG
560 570 580 590 600
PFQQAVSAVS TLNVTWYEFG EYGGYFQKYA IEYLNDDDNG YIRWFVGDTP
610 620 630 640 650
TYTIHAKALH PDGNIGWRRI SKEPMSIILN LGISNNWAYI DWQYIFFPVV
660 670 680 690 700
MSIDYVRIYQ PSNAISVTCD PSDYPTYDYI QSHLNAFQNA NLTTWEDAGY
710 720
TFPKNILTGK CTSSKFKLSS
Length:720
Mass (Da):80,123
Last modified:February 21, 2006 - v2
Checksum:iA7A91D0C40805106
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti310 – 3101D → E in AAA34726. (PubMed:1837148)Curated
Sequence conflicti310 – 3101D → E in AAB59312. (PubMed:7871892)Curated
Sequence conflicti483 – 4831G → V in AAA34726. (PubMed:1837148)Curated
Sequence conflicti483 – 4831G → V in AAB59312. (PubMed:7871892)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M80657 Genomic DNA. Translation: AAA34726.1.
L33835 Genomic DNA. Translation: AAB59312.1.
U28371 Genomic DNA. Translation: AAB68056.1.
BK006949 Genomic DNA. Translation: DAA11575.1.
PIRiS61143.
RefSeqiNP_015485.1. NM_001184256.1.

Genome annotation databases

EnsemblFungiiYPR159W; YPR159W; YPR159W.
GeneIDi856287.
KEGGisce:YPR159W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M80657 Genomic DNA. Translation: AAA34726.1 .
L33835 Genomic DNA. Translation: AAB59312.1 .
U28371 Genomic DNA. Translation: AAB68056.1 .
BK006949 Genomic DNA. Translation: DAA11575.1 .
PIRi S61143.
RefSeqi NP_015485.1. NM_001184256.1.

3D structure databases

ProteinModelPortali P32486.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36331. 71 interactions.
DIPi DIP-1850N.
IntActi P32486. 10 interactions.
MINTi MINT-720927.
STRINGi 4932.YPR159W.

Protein family/group databases

CAZyi GH16. Glycoside Hydrolase Family 16.

Proteomic databases

MaxQBi P32486.
PaxDbi P32486.
PeptideAtlasi P32486.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPR159W ; YPR159W ; YPR159W .
GeneIDi 856287.
KEGGi sce:YPR159W.

Organism-specific databases

CYGDi YPR159w.
SGDi S000006363. KRE6.

Phylogenomic databases

eggNOGi COG2273.
GeneTreei ENSGT00390000008087.
HOGENOMi HOG000164283.
InParanoidi P32486.
OMAi KVCFTQG.
OrthoDBi EOG7S4XFR.

Enzyme and pathway databases

BioCyci YEAST:YPR159W-MONOMER.

Miscellaneous databases

NextBioi 981621.

Gene expression databases

Genevestigatori P32486.

Family and domain databases

Gene3Di 2.60.120.200. 3 hits.
InterProi IPR013320. ConA-like_dom.
IPR005629. SKN1.
[Graphical view ]
Pfami PF03935. SKN1. 1 hit.
[Graphical view ]
SUPFAMi SSF49899. SSF49899. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast beta-glucan synthesis: KRE6 encodes a predicted type II membrane protein required for glucan synthesis in vivo and for glucan synthase activity in vitro."
    Roemer T., Bussey H.
    Proc. Natl. Acad. Sci. U.S.A. 88:11295-11299(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "DNA sequence analysis of a 10.4 kbp region on the right arm of yeast chromosome XVI positions GPH1 and SGV1 adjacent to KRE6, and identifies two novel tRNA genes."
    Roemer T.D., Fortin N., Bussey H.
    Yeast 10:1527-1530(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Characterization of the yeast (1-->6)-beta-glucan biosynthetic components, Kre6p and Skn1p, and genetic interactions between the PKC1 pathway and extracellular matrix assembly."
    Roemer T., Paravicini G., Payton M.A., Bussey H.
    J. Cell Biol. 127:567-579(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "Localization of synthesis of beta1,6-glucan in Saccharomyces cerevisiae."
    Montijn R.C., Vink E., Mueller W.H., Verkleij A.J., Van Den Ende H., Henrissat B., Klis F.M.
    J. Bacteriol. 181:7414-7420(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Actin patch assembly proteins Las17p and Sla1p restrict cell wall growth to daughter cells and interact with cis-Golgi protein Kre6p."
    Li H., Page N., Bussey H.
    Yeast 19:1097-1112(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH LAS17 AND SLA1.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Incorporation of Sed1p into the cell wall of Saccharomyces cerevisiae involves KRE6."
    Bowen S., Wheals A.E.
    FEMS Yeast Res. 4:731-735(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "KEG1/YFR042w encodes a novel Kre6-binding endoplasmic reticulum membrane protein responsible for beta-1,6-glucan synthesis in Saccharomyces cerevisiae."
    Nakamata K., Kurita T., Bhuiyan M.S.A., Sato K., Noda Y., Yoda K.
    J. Biol. Chem. 282:34315-34324(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KEG1.
  11. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-134, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-81; SER-116; SER-133; SER-134; SER-136 AND SER-139, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKRE6_YEAST
AccessioniPrimary (citable) accession number: P32486
Secondary accession number(s): D6W4F9, Q06472
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 21, 2006
Last modified: October 29, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4760 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3