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Protein

Beta-glucan synthesis-associated protein KRE6

Gene

KRE6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the synthesis of (1->6)- and (1->3)-beta-D-glucan polymers of the yeast cell wall in vivo. It is required for full activity of beta-glucan synthase in vitro. May be involved in the maturation and transport of cell wall proteins (CWP) to the cell wall. May act as a transglucosidase and contribute to the construction of a protein-bound glucan-structure that acts as an acceptor site for the addition of (1->6)-beta-D-glucan at the cell surface.3 Publications

Miscellaneous

Present with 4760 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • glucosidase activity Source: SGD

GO - Biological processi

  • (1->6)-beta-D-glucan biosynthetic process Source: SGD
  • fungal-type cell wall organization Source: SGD

Keywordsi

Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:YPR159W-MONOMER

Protein family/group databases

CAZyiGH16 Glycoside Hydrolase Family 16

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucan synthesis-associated protein KRE6
Alternative name(s):
Killer toxin-resistance protein 6
Gene namesi
Name:KRE6
Synonyms:CWH48
Ordered Locus Names:YPR159W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR159W
SGDiS000006363 KRE6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 252CytoplasmicSequence analysisAdd BLAST252
Transmembranei253 – 273Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini274 – 720LumenalSequence analysisAdd BLAST447

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000843311 – 720Beta-glucan synthesis-associated protein KRE6Add BLAST720

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei81PhosphoserineCombined sources1
Modified residuei116PhosphoserineCombined sources1
Modified residuei133PhosphoserineCombined sources1
Modified residuei134PhosphoserineCombined sources1
Modified residuei136PhosphoserineCombined sources1
Modified residuei139PhosphoserineCombined sources1
Glycosylationi374N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi461N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi538N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi563N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi691N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP32486
PaxDbiP32486
PRIDEiP32486

PTM databases

iPTMnetiP32486

Interactioni

Subunit structurei

The cytoplasmic domain interacts with the actin patch assembly proteins LAS17 and SLA1. Interacts with KEG1.2 Publications

Protein-protein interaction databases

BioGridi36331, 79 interactors
DIPiDIP-1850N
IntActiP32486, 14 interactors
MINTiP32486
STRINGi4932.YPR159W

Structurei

3D structure databases

ProteinModelPortaliP32486
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini289 – 664GH16PROSITE-ProRule annotationAdd BLAST376

Sequence similaritiesi

Belongs to the SKN1/KRE6 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000008087
HOGENOMiHOG000164283
InParanoidiP32486
KOiK21850
OMAiDDMFQGS
OrthoDBiEOG092C1IIF

Family and domain databases

CDDicd02180 GH16_fungal_KRE6_glucanase, 1 hit
InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR000757 GH16
IPR005629 SKN1
PANTHERiPTHR31361 PTHR31361, 1 hit
PfamiView protein in Pfam
PF03935 SKN1, 1 hit
SUPFAMiSSF49899 SSF49899, 2 hits
PROSITEiView protein in PROSITE
PS51762 GH16_2, 1 hit

Sequencei

Sequence statusi: Complete.

P32486-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLRNLTETH NFSSTNLDTD GTGDDHDGAP LSSSPSFGQQ NDNSTNDNAG
60 70 80 90 100
LTNPFMGSDE ESNARDGESL SSSVHYQPQG SDSSLLHDNS RLDLSQNKGV
110 120 130 140 150
SDYKGYYSRN NSRAVSTAND NSFLQPPHRA IASSPSLNSN LSKNDILSPP
160 170 180 190 200
EFDRYPLVGS RVTSMTQLNH HGRSPTSSPG NESSASFSSN PFLGEQDFSP
210 220 230 240 250
FGGYPASSFP LMIDEKEEDD YLHNPDPEEE ARLDRRRFID DFKYMDKRSA
260 270 280 290 300
SGLAGVLLLF LAAIFIFIVL PALTFTGAID HESNTEEVTY LTQYQYPQLS
310 320 330 340 350
AIRTSLVDPD TPDTAKTREA MDGSKWELVF SDEFNAEGRT FYDGDDPYWT
360 370 380 390 400
APDVHYDATK DLEWYSPDAS TTVNGTLQLR MDAFKNHGLY YRSGMLQSWN
410 420 430 440 450
KVCFTQGALE ISANLPNYGR VSGLWPGLWT MGNLGRPGYL ASTQGVWPYS
460 470 480 490 500
YESCDAGITP NQSSPDGISY LPGQKLSICT CDGEDHPNQG VGRGAPEIDV
510 520 530 540 550
LEGETDTKIG VGIASQSLQI APFDIWYMPD YDFIEVYNFT TTTMNTYAGG
560 570 580 590 600
PFQQAVSAVS TLNVTWYEFG EYGGYFQKYA IEYLNDDDNG YIRWFVGDTP
610 620 630 640 650
TYTIHAKALH PDGNIGWRRI SKEPMSIILN LGISNNWAYI DWQYIFFPVV
660 670 680 690 700
MSIDYVRIYQ PSNAISVTCD PSDYPTYDYI QSHLNAFQNA NLTTWEDAGY
710 720
TFPKNILTGK CTSSKFKLSS
Length:720
Mass (Da):80,123
Last modified:February 21, 2006 - v2
Checksum:iA7A91D0C40805106
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti310D → E in AAA34726 (PubMed:1837148).Curated1
Sequence conflicti310D → E in AAB59312 (PubMed:7871892).Curated1
Sequence conflicti483G → V in AAA34726 (PubMed:1837148).Curated1
Sequence conflicti483G → V in AAB59312 (PubMed:7871892).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80657 Genomic DNA Translation: AAA34726.1
L33835 Genomic DNA Translation: AAB59312.1
U28371 Genomic DNA Translation: AAB68056.1
BK006949 Genomic DNA Translation: DAA11575.1
PIRiS61143
RefSeqiNP_015485.1, NM_001184256.1

Genome annotation databases

EnsemblFungiiYPR159W; YPR159W; YPR159W
GeneIDi856287
KEGGisce:YPR159W

Similar proteinsi

Entry informationi

Entry nameiKRE6_YEAST
AccessioniPrimary (citable) accession number: P32486
Secondary accession number(s): D6W4F9, Q06472
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 21, 2006
Last modified: May 23, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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