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P32486

- KRE6_YEAST

UniProt

P32486 - KRE6_YEAST

Protein

Beta-glucan synthesis-associated protein KRE6

Gene

KRE6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 2 (21 Feb 2006)
      Previous versions | rss
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    Functioni

    Involved in the synthesis of (1->6)- and (1->3)-beta-D-glucan polymers of the yeast cell wall in vivo. It is required for full activity of beta-glucan synthase in vitro. May be involved in the maturation and transport of cell wall proteins (CWP) to the cell wall. May act as a transglucosidase and contribute to the construction of a protein-bound glucan-structure that acts as an acceptor site for the addition of (1->6)-beta-D-glucan at the cell surface.3 Publications

    GO - Molecular functioni

    1. glucosidase activity Source: SGD

    GO - Biological processi

    1. (1->6)-beta-D-glucan biosynthetic process Source: SGD
    2. fungal-type cell wall organization Source: SGD

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciYEAST:YPR159W-MONOMER.

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucan synthesis-associated protein KRE6
    Alternative name(s):
    Killer toxin-resistance protein 6
    Gene namesi
    Name:KRE6
    Synonyms:CWH48
    Ordered Locus Names:YPR159W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPR159w.
    SGDiS000006363. KRE6.

    Subcellular locationi

    Golgi apparatus membrane 1 Publication; Single-pass type II membrane protein 1 Publication

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of endoplasmic reticulum membrane Source: SGD
    3. integral component of membrane Source: SGD
    4. plasma membrane Source: SGD
    5. site of polarized growth Source: SGD
    6. transport vesicle Source: SGD

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 720720Beta-glucan synthesis-associated protein KRE6PRO_0000084331Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei81 – 811Phosphoserine1 Publication
    Modified residuei116 – 1161Phosphoserine1 Publication
    Modified residuei133 – 1331Phosphoserine1 Publication
    Modified residuei134 – 1341Phosphoserine2 Publications
    Modified residuei136 – 1361Phosphoserine1 Publication
    Modified residuei139 – 1391Phosphoserine1 Publication
    Glycosylationi374 – 3741N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi461 – 4611N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi538 – 5381N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi563 – 5631N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi691 – 6911N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP32486.
    PaxDbiP32486.
    PeptideAtlasiP32486.

    Expressioni

    Gene expression databases

    GenevestigatoriP32486.

    Interactioni

    Subunit structurei

    The cytoplasmic domain interacts with the actin patch assembly proteins LAS17 and SLA1. Interacts with KEG1.2 Publications

    Protein-protein interaction databases

    BioGridi36331. 71 interactions.
    DIPiDIP-1850N.
    IntActiP32486. 10 interactions.
    MINTiMINT-720927.
    STRINGi4932.YPR159W.

    Structurei

    3D structure databases

    ProteinModelPortaliP32486.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 252252CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini274 – 720447LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei253 – 27321Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the SKN1/KRE6 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2273.
    GeneTreeiENSGT00390000008087.
    HOGENOMiHOG000164283.
    OMAiKVCFTQG.
    OrthoDBiEOG7S4XFR.

    Family and domain databases

    Gene3Di2.60.120.200. 3 hits.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR005629. SKN1.
    [Graphical view]
    PfamiPF03935. SKN1. 1 hit.
    [Graphical view]
    SUPFAMiSSF49899. SSF49899. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P32486-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPLRNLTETH NFSSTNLDTD GTGDDHDGAP LSSSPSFGQQ NDNSTNDNAG    50
    LTNPFMGSDE ESNARDGESL SSSVHYQPQG SDSSLLHDNS RLDLSQNKGV 100
    SDYKGYYSRN NSRAVSTAND NSFLQPPHRA IASSPSLNSN LSKNDILSPP 150
    EFDRYPLVGS RVTSMTQLNH HGRSPTSSPG NESSASFSSN PFLGEQDFSP 200
    FGGYPASSFP LMIDEKEEDD YLHNPDPEEE ARLDRRRFID DFKYMDKRSA 250
    SGLAGVLLLF LAAIFIFIVL PALTFTGAID HESNTEEVTY LTQYQYPQLS 300
    AIRTSLVDPD TPDTAKTREA MDGSKWELVF SDEFNAEGRT FYDGDDPYWT 350
    APDVHYDATK DLEWYSPDAS TTVNGTLQLR MDAFKNHGLY YRSGMLQSWN 400
    KVCFTQGALE ISANLPNYGR VSGLWPGLWT MGNLGRPGYL ASTQGVWPYS 450
    YESCDAGITP NQSSPDGISY LPGQKLSICT CDGEDHPNQG VGRGAPEIDV 500
    LEGETDTKIG VGIASQSLQI APFDIWYMPD YDFIEVYNFT TTTMNTYAGG 550
    PFQQAVSAVS TLNVTWYEFG EYGGYFQKYA IEYLNDDDNG YIRWFVGDTP 600
    TYTIHAKALH PDGNIGWRRI SKEPMSIILN LGISNNWAYI DWQYIFFPVV 650
    MSIDYVRIYQ PSNAISVTCD PSDYPTYDYI QSHLNAFQNA NLTTWEDAGY 700
    TFPKNILTGK CTSSKFKLSS 720
    Length:720
    Mass (Da):80,123
    Last modified:February 21, 2006 - v2
    Checksum:iA7A91D0C40805106
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti310 – 3101D → E in AAA34726. (PubMed:1837148)Curated
    Sequence conflicti310 – 3101D → E in AAB59312. (PubMed:7871892)Curated
    Sequence conflicti483 – 4831G → V in AAA34726. (PubMed:1837148)Curated
    Sequence conflicti483 – 4831G → V in AAB59312. (PubMed:7871892)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M80657 Genomic DNA. Translation: AAA34726.1.
    L33835 Genomic DNA. Translation: AAB59312.1.
    U28371 Genomic DNA. Translation: AAB68056.1.
    BK006949 Genomic DNA. Translation: DAA11575.1.
    PIRiS61143.
    RefSeqiNP_015485.1. NM_001184256.1.

    Genome annotation databases

    EnsemblFungiiYPR159W; YPR159W; YPR159W.
    GeneIDi856287.
    KEGGisce:YPR159W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M80657 Genomic DNA. Translation: AAA34726.1 .
    L33835 Genomic DNA. Translation: AAB59312.1 .
    U28371 Genomic DNA. Translation: AAB68056.1 .
    BK006949 Genomic DNA. Translation: DAA11575.1 .
    PIRi S61143.
    RefSeqi NP_015485.1. NM_001184256.1.

    3D structure databases

    ProteinModelPortali P32486.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36331. 71 interactions.
    DIPi DIP-1850N.
    IntActi P32486. 10 interactions.
    MINTi MINT-720927.
    STRINGi 4932.YPR159W.

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Proteomic databases

    MaxQBi P32486.
    PaxDbi P32486.
    PeptideAtlasi P32486.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPR159W ; YPR159W ; YPR159W .
    GeneIDi 856287.
    KEGGi sce:YPR159W.

    Organism-specific databases

    CYGDi YPR159w.
    SGDi S000006363. KRE6.

    Phylogenomic databases

    eggNOGi COG2273.
    GeneTreei ENSGT00390000008087.
    HOGENOMi HOG000164283.
    OMAi KVCFTQG.
    OrthoDBi EOG7S4XFR.

    Enzyme and pathway databases

    BioCyci YEAST:YPR159W-MONOMER.

    Miscellaneous databases

    NextBioi 981621.

    Gene expression databases

    Genevestigatori P32486.

    Family and domain databases

    Gene3Di 2.60.120.200. 3 hits.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR005629. SKN1.
    [Graphical view ]
    Pfami PF03935. SKN1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49899. SSF49899. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Yeast beta-glucan synthesis: KRE6 encodes a predicted type II membrane protein required for glucan synthesis in vivo and for glucan synthase activity in vitro."
      Roemer T., Bussey H.
      Proc. Natl. Acad. Sci. U.S.A. 88:11295-11299(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "DNA sequence analysis of a 10.4 kbp region on the right arm of yeast chromosome XVI positions GPH1 and SGV1 adjacent to KRE6, and identifies two novel tRNA genes."
      Roemer T.D., Fortin N., Bussey H.
      Yeast 10:1527-1530(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Characterization of the yeast (1-->6)-beta-glucan biosynthetic components, Kre6p and Skn1p, and genetic interactions between the PKC1 pathway and extracellular matrix assembly."
      Roemer T., Paravicini G., Payton M.A., Bussey H.
      J. Cell Biol. 127:567-579(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. "Localization of synthesis of beta1,6-glucan in Saccharomyces cerevisiae."
      Montijn R.C., Vink E., Mueller W.H., Verkleij A.J., Van Den Ende H., Henrissat B., Klis F.M.
      J. Bacteriol. 181:7414-7420(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Actin patch assembly proteins Las17p and Sla1p restrict cell wall growth to daughter cells and interact with cis-Golgi protein Kre6p."
      Li H., Page N., Bussey H.
      Yeast 19:1097-1112(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH LAS17 AND SLA1.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Incorporation of Sed1p into the cell wall of Saccharomyces cerevisiae involves KRE6."
      Bowen S., Wheals A.E.
      FEMS Yeast Res. 4:731-735(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "KEG1/YFR042w encodes a novel Kre6-binding endoplasmic reticulum membrane protein responsible for beta-1,6-glucan synthesis in Saccharomyces cerevisiae."
      Nakamata K., Kurita T., Bhuiyan M.S.A., Sato K., Noda Y., Yoda K.
      J. Biol. Chem. 282:34315-34324(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH KEG1.
    11. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-134, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-81; SER-116; SER-133; SER-134; SER-136 AND SER-139, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiKRE6_YEAST
    AccessioniPrimary (citable) accession number: P32486
    Secondary accession number(s): D6W4F9, Q06472
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: February 21, 2006
    Last modified: October 1, 2014
    This is version 125 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4760 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3