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P32486 (KRE6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-glucan synthesis-associated protein KRE6
Alternative name(s):
Killer toxin-resistance protein 6
Gene names
Name:KRE6
Synonyms:CWH48
Ordered Locus Names:YPR159W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length720 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the synthesis of (1->6)- and (1->3)-beta-D-glucan polymers of the yeast cell wall in vivo. It is required for full activity of beta-glucan synthase in vitro. May be involved in the maturation and transport of cell wall proteins (CWP) to the cell wall. May act as a transglucosidase and contribute to the construction of a protein-bound glucan-structure that acts as an acceptor site for the addition of (1->6)-beta-D-glucan at the cell surface. Ref.6 Ref.7 Ref.9

Subunit structure

The cytoplasmic domain interacts with the actin patch assembly proteins LAS17 and SLA1. Interacts with KEG1. Ref.7 Ref.10

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein Ref.7.

Miscellaneous

Present with 4760 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the SKN1/KRE6 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 720720Beta-glucan synthesis-associated protein KRE6
PRO_0000084331

Regions

Topological domain1 – 252252Cytoplasmic Potential
Transmembrane253 – 27321Helical; Signal-anchor for type II membrane protein; Potential
Topological domain274 – 720447Lumenal Potential

Amino acid modifications

Modified residue811Phosphoserine Ref.13
Modified residue1161Phosphoserine Ref.13
Modified residue1331Phosphoserine Ref.13
Modified residue1341Phosphoserine Ref.11 Ref.13
Modified residue1361Phosphoserine Ref.13
Modified residue1391Phosphoserine Ref.13
Glycosylation3741N-linked (GlcNAc...) Potential
Glycosylation4611N-linked (GlcNAc...) Potential
Glycosylation5381N-linked (GlcNAc...) Potential
Glycosylation5631N-linked (GlcNAc...) Potential
Glycosylation6911N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict3101D → E in AAA34726. Ref.1
Sequence conflict3101D → E in AAB59312. Ref.2
Sequence conflict4831G → V in AAA34726. Ref.1
Sequence conflict4831G → V in AAB59312. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P32486 [UniParc].

Last modified February 21, 2006. Version 2.
Checksum: A7A91D0C40805106

FASTA72080,123
        10         20         30         40         50         60 
MPLRNLTETH NFSSTNLDTD GTGDDHDGAP LSSSPSFGQQ NDNSTNDNAG LTNPFMGSDE 

        70         80         90        100        110        120 
ESNARDGESL SSSVHYQPQG SDSSLLHDNS RLDLSQNKGV SDYKGYYSRN NSRAVSTAND 

       130        140        150        160        170        180 
NSFLQPPHRA IASSPSLNSN LSKNDILSPP EFDRYPLVGS RVTSMTQLNH HGRSPTSSPG 

       190        200        210        220        230        240 
NESSASFSSN PFLGEQDFSP FGGYPASSFP LMIDEKEEDD YLHNPDPEEE ARLDRRRFID 

       250        260        270        280        290        300 
DFKYMDKRSA SGLAGVLLLF LAAIFIFIVL PALTFTGAID HESNTEEVTY LTQYQYPQLS 

       310        320        330        340        350        360 
AIRTSLVDPD TPDTAKTREA MDGSKWELVF SDEFNAEGRT FYDGDDPYWT APDVHYDATK 

       370        380        390        400        410        420 
DLEWYSPDAS TTVNGTLQLR MDAFKNHGLY YRSGMLQSWN KVCFTQGALE ISANLPNYGR 

       430        440        450        460        470        480 
VSGLWPGLWT MGNLGRPGYL ASTQGVWPYS YESCDAGITP NQSSPDGISY LPGQKLSICT 

       490        500        510        520        530        540 
CDGEDHPNQG VGRGAPEIDV LEGETDTKIG VGIASQSLQI APFDIWYMPD YDFIEVYNFT 

       550        560        570        580        590        600 
TTTMNTYAGG PFQQAVSAVS TLNVTWYEFG EYGGYFQKYA IEYLNDDDNG YIRWFVGDTP 

       610        620        630        640        650        660 
TYTIHAKALH PDGNIGWRRI SKEPMSIILN LGISNNWAYI DWQYIFFPVV MSIDYVRIYQ 

       670        680        690        700        710        720 
PSNAISVTCD PSDYPTYDYI QSHLNAFQNA NLTTWEDAGY TFPKNILTGK CTSSKFKLSS 

« Hide

References

« Hide 'large scale' references
[1]"Yeast beta-glucan synthesis: KRE6 encodes a predicted type II membrane protein required for glucan synthesis in vivo and for glucan synthase activity in vitro."
Roemer T., Bussey H.
Proc. Natl. Acad. Sci. U.S.A. 88:11295-11299(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"DNA sequence analysis of a 10.4 kbp region on the right arm of yeast chromosome XVI positions GPH1 and SGV1 adjacent to KRE6, and identifies two novel tRNA genes."
Roemer T.D., Fortin N., Bussey H.
Yeast 10:1527-1530(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Characterization of the yeast (1-->6)-beta-glucan biosynthetic components, Kre6p and Skn1p, and genetic interactions between the PKC1 pathway and extracellular matrix assembly."
Roemer T., Paravicini G., Payton M.A., Bussey H.
J. Cell Biol. 127:567-579(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Localization of synthesis of beta1,6-glucan in Saccharomyces cerevisiae."
Montijn R.C., Vink E., Mueller W.H., Verkleij A.J., Van Den Ende H., Henrissat B., Klis F.M.
J. Bacteriol. 181:7414-7420(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Actin patch assembly proteins Las17p and Sla1p restrict cell wall growth to daughter cells and interact with cis-Golgi protein Kre6p."
Li H., Page N., Bussey H.
Yeast 19:1097-1112(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH LAS17 AND SLA1.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Incorporation of Sed1p into the cell wall of Saccharomyces cerevisiae involves KRE6."
Bowen S., Wheals A.E.
FEMS Yeast Res. 4:731-735(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"KEG1/YFR042w encodes a novel Kre6-binding endoplasmic reticulum membrane protein responsible for beta-1,6-glucan synthesis in Saccharomyces cerevisiae."
Nakamata K., Kurita T., Bhuiyan M.S.A., Sato K., Noda Y., Yoda K.
J. Biol. Chem. 282:34315-34324(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH KEG1.
[11]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-134, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[12]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-81; SER-116; SER-133; SER-134; SER-136 AND SER-139, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M80657 Genomic DNA. Translation: AAA34726.1.
L33835 Genomic DNA. Translation: AAB59312.1.
U28371 Genomic DNA. Translation: AAB68056.1.
BK006949 Genomic DNA. Translation: DAA11575.1.
PIRS61143.
RefSeqNP_015485.1. NM_001184256.1.

3D structure databases

ProteinModelPortalP32486.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36331. 71 interactions.
DIPDIP-1850N.
IntActP32486. 10 interactions.
MINTMINT-720927.
STRING4932.YPR159W.

Protein family/group databases

CAZyGH16. Glycoside Hydrolase Family 16.

Proteomic databases

PaxDbP32486.
PeptideAtlasP32486.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPR159W; YPR159W; YPR159W.
GeneID856287.
KEGGsce:YPR159W.

Organism-specific databases

CYGDYPR159w.
SGDS000006363. KRE6.

Phylogenomic databases

eggNOGCOG2273.
GeneTreeENSGT00390000008087.
HOGENOMHOG000164283.
OMAKVCFTQG.
OrthoDBEOG7S4XFR.

Enzyme and pathway databases

BioCycYEAST:YPR159W-MONOMER.

Gene expression databases

GenevestigatorP32486.

Family and domain databases

Gene3D2.60.120.200. 3 hits.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR005629. SKN1.
[Graphical view]
PfamPF03935. SKN1. 1 hit.
[Graphical view]
SUPFAMSSF49899. SSF49899. 2 hits.
ProtoNetSearch...

Other

NextBio981621.

Entry information

Entry nameKRE6_YEAST
AccessionPrimary (citable) accession number: P32486
Secondary accession number(s): D6W4F9, Q06472
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 21, 2006
Last modified: April 16, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families