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Protein

Beta-glucan synthesis-associated protein KRE6

Gene

KRE6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the synthesis of (1->6)- and (1->3)-beta-D-glucan polymers of the yeast cell wall in vivo. It is required for full activity of beta-glucan synthase in vitro. May be involved in the maturation and transport of cell wall proteins (CWP) to the cell wall. May act as a transglucosidase and contribute to the construction of a protein-bound glucan-structure that acts as an acceptor site for the addition of (1->6)-beta-D-glucan at the cell surface.3 Publications

GO - Molecular functioni

  • glucosidase activity Source: SGD

GO - Biological processi

  • (1->6)-beta-D-glucan biosynthetic process Source: SGD
  • fungal-type cell wall organization Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:YPR159W-MONOMER.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucan synthesis-associated protein KRE6
Alternative name(s):
Killer toxin-resistance protein 6
Gene namesi
Name:KRE6
Synonyms:CWH48
Ordered Locus Names:YPR159W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR159W.
SGDiS000006363. KRE6.

Subcellular locationi

  • Golgi apparatus membrane 1 Publication; Single-pass type II membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 252CytoplasmicSequence analysisAdd BLAST252
Transmembranei253 – 273Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini274 – 720LumenalSequence analysisAdd BLAST447

GO - Cellular componenti

  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of endoplasmic reticulum membrane Source: SGD
  • integral component of membrane Source: SGD
  • plasma membrane Source: SGD
  • site of polarized growth Source: SGD
  • transport vesicle Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000843311 – 720Beta-glucan synthesis-associated protein KRE6Add BLAST720

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei81PhosphoserineCombined sources1
Modified residuei116PhosphoserineCombined sources1
Modified residuei133PhosphoserineCombined sources1
Modified residuei134PhosphoserineCombined sources1
Modified residuei136PhosphoserineCombined sources1
Modified residuei139PhosphoserineCombined sources1
Glycosylationi374N-linked (GlcNAc...)Sequence analysis1
Glycosylationi461N-linked (GlcNAc...)Sequence analysis1
Glycosylationi538N-linked (GlcNAc...)Sequence analysis1
Glycosylationi563N-linked (GlcNAc...)Sequence analysis1
Glycosylationi691N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP32486.
PRIDEiP32486.

PTM databases

iPTMnetiP32486.

Interactioni

Subunit structurei

The cytoplasmic domain interacts with the actin patch assembly proteins LAS17 and SLA1. Interacts with KEG1.2 Publications

Protein-protein interaction databases

BioGridi36331. 73 interactors.
DIPiDIP-1850N.
IntActiP32486. 10 interactors.
MINTiMINT-720927.

Structurei

3D structure databases

ProteinModelPortaliP32486.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini289 – 664GH16PROSITE-ProRule annotationAdd BLAST376

Sequence similaritiesi

Belongs to the SKN1/KRE6 family.Curated
Contains 1 GH16 (glycosyl hydrolase family 16) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000008087.
HOGENOMiHOG000164283.
InParanoidiP32486.
OMAiVTSMNTY.
OrthoDBiEOG092C1IIF.

Family and domain databases

Gene3Di2.60.120.200. 3 hits.
InterProiIPR013320. ConA-like_dom.
IPR000757. GH16.
IPR005629. SKN1.
[Graphical view]
PfamiPF03935. SKN1. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
PROSITEiPS51762. GH16_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32486-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLRNLTETH NFSSTNLDTD GTGDDHDGAP LSSSPSFGQQ NDNSTNDNAG
60 70 80 90 100
LTNPFMGSDE ESNARDGESL SSSVHYQPQG SDSSLLHDNS RLDLSQNKGV
110 120 130 140 150
SDYKGYYSRN NSRAVSTAND NSFLQPPHRA IASSPSLNSN LSKNDILSPP
160 170 180 190 200
EFDRYPLVGS RVTSMTQLNH HGRSPTSSPG NESSASFSSN PFLGEQDFSP
210 220 230 240 250
FGGYPASSFP LMIDEKEEDD YLHNPDPEEE ARLDRRRFID DFKYMDKRSA
260 270 280 290 300
SGLAGVLLLF LAAIFIFIVL PALTFTGAID HESNTEEVTY LTQYQYPQLS
310 320 330 340 350
AIRTSLVDPD TPDTAKTREA MDGSKWELVF SDEFNAEGRT FYDGDDPYWT
360 370 380 390 400
APDVHYDATK DLEWYSPDAS TTVNGTLQLR MDAFKNHGLY YRSGMLQSWN
410 420 430 440 450
KVCFTQGALE ISANLPNYGR VSGLWPGLWT MGNLGRPGYL ASTQGVWPYS
460 470 480 490 500
YESCDAGITP NQSSPDGISY LPGQKLSICT CDGEDHPNQG VGRGAPEIDV
510 520 530 540 550
LEGETDTKIG VGIASQSLQI APFDIWYMPD YDFIEVYNFT TTTMNTYAGG
560 570 580 590 600
PFQQAVSAVS TLNVTWYEFG EYGGYFQKYA IEYLNDDDNG YIRWFVGDTP
610 620 630 640 650
TYTIHAKALH PDGNIGWRRI SKEPMSIILN LGISNNWAYI DWQYIFFPVV
660 670 680 690 700
MSIDYVRIYQ PSNAISVTCD PSDYPTYDYI QSHLNAFQNA NLTTWEDAGY
710 720
TFPKNILTGK CTSSKFKLSS
Length:720
Mass (Da):80,123
Last modified:February 21, 2006 - v2
Checksum:iA7A91D0C40805106
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti310D → E in AAA34726 (PubMed:1837148).Curated1
Sequence conflicti310D → E in AAB59312 (PubMed:7871892).Curated1
Sequence conflicti483G → V in AAA34726 (PubMed:1837148).Curated1
Sequence conflicti483G → V in AAB59312 (PubMed:7871892).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80657 Genomic DNA. Translation: AAA34726.1.
L33835 Genomic DNA. Translation: AAB59312.1.
U28371 Genomic DNA. Translation: AAB68056.1.
BK006949 Genomic DNA. Translation: DAA11575.1.
PIRiS61143.
RefSeqiNP_015485.1. NM_001184256.1.

Genome annotation databases

EnsemblFungiiYPR159W; YPR159W; YPR159W.
GeneIDi856287.
KEGGisce:YPR159W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80657 Genomic DNA. Translation: AAA34726.1.
L33835 Genomic DNA. Translation: AAB59312.1.
U28371 Genomic DNA. Translation: AAB68056.1.
BK006949 Genomic DNA. Translation: DAA11575.1.
PIRiS61143.
RefSeqiNP_015485.1. NM_001184256.1.

3D structure databases

ProteinModelPortaliP32486.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36331. 73 interactors.
DIPiDIP-1850N.
IntActiP32486. 10 interactors.
MINTiMINT-720927.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

PTM databases

iPTMnetiP32486.

Proteomic databases

MaxQBiP32486.
PRIDEiP32486.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR159W; YPR159W; YPR159W.
GeneIDi856287.
KEGGisce:YPR159W.

Organism-specific databases

EuPathDBiFungiDB:YPR159W.
SGDiS000006363. KRE6.

Phylogenomic databases

GeneTreeiENSGT00390000008087.
HOGENOMiHOG000164283.
InParanoidiP32486.
OMAiVTSMNTY.
OrthoDBiEOG092C1IIF.

Enzyme and pathway databases

BioCyciYEAST:YPR159W-MONOMER.

Miscellaneous databases

PROiP32486.

Family and domain databases

Gene3Di2.60.120.200. 3 hits.
InterProiIPR013320. ConA-like_dom.
IPR000757. GH16.
IPR005629. SKN1.
[Graphical view]
PfamiPF03935. SKN1. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
PROSITEiPS51762. GH16_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKRE6_YEAST
AccessioniPrimary (citable) accession number: P32486
Secondary accession number(s): D6W4F9, Q06472
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 21, 2006
Last modified: November 2, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4760 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.