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P32485

- HOG1_YEAST

UniProt

P32485 - HOG1_YEAST

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Protein
Mitogen-activated protein kinase HOG1
Gene
HOG1, SSK3, YLR113W, L2931, L9354.2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription via the stress response element (STRE) in promoters of target genes. Upon osmotic shock, associates with the SKO1-SSN6-TUP1 complex, phosphorylates SKO1, and converts it into an activator that subsequently recruits Swi/Snf and SAGA complexes. Activates the SMP1 transcription factor and the RCK2 kinase, both also involved in the regulation of the expression of a subset of osmotic stress-related genes. Phosphorylation of HSL1 by HOG1 leads to a G2 arrest essential for cell survival at high osmolarity. Mediates also cell-cycle arrest in G1 phase by the dual targeting of SIC1. Regulates MFA2 ARE-mediated translation in response to carbon source. Targets RDP3 histone deacetylase to osmoresponsive promoters to induce gene expression on stress. Plays an essential role in maintaining water homeostasis, arsenite detoxification, copper-resistance, hydrogen peroxide response, adaptation to citric acid stress, and repression of the mating pathway activity. Required for the Golgi apparatus localization of MNN1.36 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium By similarity.

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation. Is phosphorylated on Tyr-176 by PBS2. Inactivated by dephosphorylation by NBP2 after adaptation to osmotic stress. PTP2 and PTP3 inactivate HOG1 by dephosphorylating Tyr-176, while the PP2Cs PTC1 and PTC2 or PTC3 dephosphorylate Thr-174 in the activation loop.5 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei52 – 521ATP By similarity
Active sitei144 – 1441Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 379ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: SGD
  3. chromatin binding Source: SGD
  4. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. MAPK cascade Source: GOC
  2. cellular response to heat Source: SGD
  3. cellular response to osmotic stress Source: SGD
  4. hyperosmotic response Source: SGD
  5. osmosensory signaling pathway Source: SGD
  6. positive regulation of transcription from RNA polymerase II promoter Source: SGD
  7. positive regulation of transcription from RNA polymerase II promoter in response to osmotic stress Source: SGD
  8. protein phosphorylation Source: SGD
  9. response to arsenic-containing substance Source: SGD
  10. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32258-MONOMER.
BRENDAi2.7.11.24. 984.
ReactomeiREACT_188484. Oxidative Stress Induced Senescence.
REACT_189013. NOD1/2 Signaling Pathway.
REACT_212004. p38MAPK events.
REACT_96987. activated TAK1 mediates p38 MAPK activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase HOG1 (EC:2.7.11.24)
Short name:
MAP kinase HOG1
Alternative name(s):
High osmolarity glycerol response protein 1
Gene namesi
Name:HOG1
Synonyms:SSK3
Ordered Locus Names:YLR113W
ORF Names:L2931, L9354.2
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR113w.
SGDiS000004103. HOG1.

Subcellular locationi

Cytoplasm. Nucleus
Note: Predominantly cytoplasmic in unstressed cells but rapidly concentrates within the nucleus in response to hyperosmotic conditions and phosphorylation.7 Publications

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi52 – 521K → R: Impairs catalytic activity, nuclear translocation, expression of CTT1 and increases sensitivity to osmotic shock. 4 Publications
Mutagenesisi68 – 681Y → H: Activates HOG1 in a constitutive manner, without the need of a stimulating stress. 1 Publication
Mutagenesisi144 – 1441D → A: Impairs catalytic activity and nuclear translocation. 1 Publication
Mutagenesisi170 – 1701D → A: Activates HOG1 in a constitutive manner, without the need of a stimulating stress. 1 Publication
Mutagenesisi174 – 1741T → A: Impairs catalytic activity, expression of CTT1 and increases sensitivity to osmotic shock. 2 Publications
Mutagenesisi176 – 1761Y → F: Impairs expression of CTT1 and increases sensitivity to osmotic shock. 2 Publications
Mutagenesisi314 – 3141A → T: Activates HOG1 in a constitutive manner, without the need of a stimulating stress. 1 Publication
Mutagenesisi318 – 3181F → L or S: Activates HOG1 in a constitutive manner, without the need of a stimulating stress. 1 Publication
Mutagenesisi320 – 3201W → R: Activates HOG1 in a constitutive manner, without the need of a stimulating stress. 1 Publication
Mutagenesisi322 – 3221F → L: Activates HOG1 in a constitutive manner, without the need of a stimulating stress. 1 Publication
Mutagenesisi332 – 3321W → R: Activates HOG1 in a constitutive manner, without the need of a stimulating stress. 1 Publication
Mutagenesisi391 – 3911N → D: Activates HOG1 in a constitutive manner, without the need of a stimulating stress. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 435434Mitogen-activated protein kinase HOG1
PRO_0000186331Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonine1 Publication
Modified residuei174 – 1741Phosphothreonine1 Publication
Modified residuei176 – 1761Phosphotyrosine2 Publications

Post-translational modificationi

Dually phosphorylated on Thr-174 and Tyr-176, which activates the enzyme.14 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP32485.
PaxDbiP32485.
PeptideAtlasiP32485.

Expressioni

Inductioni

By osmotic stress, cold stress, citric acid, and in presence of bacterial lipopolysaccharides (LPS).6 Publications

Gene expression databases

GenevestigatoriP32485.

Interactioni

Subunit structurei

Interacts with CDC37, HOT1, KIN28, PTP2, PTP3, RBP1, RCK2, RPD3, SIC1, SMP1 and SIN4.10 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HOT1Q032134EBI-8437,EBI-27376
HSL1P342442EBI-8437,EBI-9771
SIC1P386344EBI-8437,EBI-17127
STE7P067842EBI-8437,EBI-18389
UBP3Q014773EBI-8437,EBI-19834

Protein-protein interaction databases

BioGridi31384. 331 interactions.
DIPiDIP-1558N.
IntActiP32485. 29 interactions.
MINTiMINT-404719.

Structurei

3D structure databases

ProteinModelPortaliP32485.
SMRiP32485. Positions 5-345.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 302280Protein kinase
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi174 – 1763TXY

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi364 – 37916Ala-rich
Add
BLAST

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000074271.
HOGENOMiHOG000233024.
KOiK04441.
OMAiPDDVIHT.
OrthoDBiEOG7K3TWD.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01773. P38MAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32485-1 [UniParc]FASTAAdd to Basket

« Hide

MTTNEEFIRT QIFGTVFEIT NRYNDLNPVG MGAFGLVCSA TDTLTSQPVA    50
IKKIMKPFST AVLAKRTYRE LKLLKHLRHE NLICLQDIFL SPLEDIYFVT 100
ELQGTDLHRL LQTRPLEKQF VQYFLYQILR GLKYVHSAGV IHRDLKPSNI 150
LINENCDLKI CDFGLARIQD PQMTGYVSTR YYRAPEIMLT WQKYDVEVDI 200
WSAGCIFAEM IEGKPLFPGK DHVHQFSIIT DLLGSPPKDV INTICSENTL 250
KFVTSLPHRD PIPFSERFKT VEPDAVDLLE KMLVFDPKKR ITAADALAHP 300
YSAPYHDPTD EPVADAKFDW HFNDADLPVD TWRVMMYSEI LDFHKIGGSD 350
GQIDISATFD DQVAAATAAA AQAQAQAQAQ VQLNMAAHSH NGAGTTGNDH 400
SDIAGGNKVS DHVAANDTIT DYGNQAIQYA NEFQQ 435
Length:435
Mass (Da):48,858
Last modified:November 1, 1997 - v2
Checksum:iD95A20242587CE06
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91R → G in AAB67558. 1 Publication
Sequence conflicti409 – 43527VSDHV…NEFQQ → GQRSCSCK in AAA34680. 1 Publication
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L06279 Genomic DNA. Translation: AAA34680.1.
U53878 Genomic DNA. Translation: AAB67558.1.
Z73285 Genomic DNA. Translation: CAA97680.1.
X89514 Genomic DNA. Translation: CAA61691.1.
BK006945 Genomic DNA. Translation: DAA09427.1.
PIRiS64950.
RefSeqiNP_013214.1. NM_001182000.1.

Genome annotation databases

EnsemblFungiiYLR113W; YLR113W; YLR113W.
GeneIDi850803.
KEGGisce:YLR113W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L06279 Genomic DNA. Translation: AAA34680.1 .
U53878 Genomic DNA. Translation: AAB67558.1 .
Z73285 Genomic DNA. Translation: CAA97680.1 .
X89514 Genomic DNA. Translation: CAA61691.1 .
BK006945 Genomic DNA. Translation: DAA09427.1 .
PIRi S64950.
RefSeqi NP_013214.1. NM_001182000.1.

3D structure databases

ProteinModelPortali P32485.
SMRi P32485. Positions 5-345.
ModBasei Search...

Protein-protein interaction databases

BioGridi 31384. 331 interactions.
DIPi DIP-1558N.
IntActi P32485. 29 interactions.
MINTi MINT-404719.

Proteomic databases

MaxQBi P32485.
PaxDbi P32485.
PeptideAtlasi P32485.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR113W ; YLR113W ; YLR113W .
GeneIDi 850803.
KEGGi sce:YLR113W.

Organism-specific databases

CYGDi YLR113w.
SGDi S000004103. HOG1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00550000074271.
HOGENOMi HOG000233024.
KOi K04441.
OMAi PDDVIHT.
OrthoDBi EOG7K3TWD.

Enzyme and pathway databases

BioCyci YEAST:G3O-32258-MONOMER.
BRENDAi 2.7.11.24. 984.
Reactomei REACT_188484. Oxidative Stress Induced Senescence.
REACT_189013. NOD1/2 Signaling Pathway.
REACT_212004. p38MAPK events.
REACT_96987. activated TAK1 mediates p38 MAPK activation.

Miscellaneous databases

NextBioi 967026.
PROi P32485.

Gene expression databases

Genevestigatori P32485.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR01773. P38MAPKINASE.
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "An osmosensing signal transduction pathway in yeast."
    Brewster J.L., de Valoir T., Dwyer N.D., Winter E., Gustin M.C.
    Science 259:1760-1763(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence analysis of a 37.6 kbp cosmid clone from the right arm of Saccharomyces cerevisiae chromosome XII, carrying YAP3, HOG1, SNR6, tRNA-Arg3 and 23 new open reading frames, among which several homologies to proteins involved in cell division control and to mammalian growth factors and other animal proteins are found."
    Verhasselt P., Volckaert G.
    Yeast 13:241-250(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 90840 / EAY235 / FY23.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The HOG pathway controls osmotic regulation of transcription via the stress response element (STRE) of the Saccharomyces cerevisiae CTT1 gene."
    Schueller C., Brewster J.L., Alexander M.R., Gustin M.C., Ruis H.
    EMBO J. 13:4382-4389(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION, MUTAGENESIS OF LYS-52; THR-174 AND TYR-176.
  6. "Purification and characterization of two isoenzymes of DL-glycerol-3-phosphatase from Saccharomyces cerevisiae. Identification of the corresponding GPP1 and GPP2 genes and evidence for osmotic regulation of Gpp2p expression by the osmosensing mitogen-activated protein kinase signal transduction pathway."
    Norbeck J., Paehlman A.-K., Akhtar N., Blomberg A., Adler L.
    J. Biol. Chem. 271:13875-13881(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "The osmoregulatory pathway represses mating pathway activity in Saccharomyces cerevisiae: isolation of a FUS3 mutant that is insensitive to the repression mechanism."
    Hall J.P., Cherkasova V., Elion E., Gustin M.C., Winter E.
    Mol. Cell. Biol. 16:6715-6723(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF LYS-52.
  8. "Regulation of the Saccharomyces cerevisiae HOG1 mitogen-activated protein kinase by the PTP2 and PTP3 protein tyrosine phosphatases."
    Wurgler-Murphy S.M., Maeda T., Witten E.A., Saito H.
    Mol. Cell. Biol. 17:1289-1297(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PTP2, ENZYME REGULATION.
  9. "Two protein-tyrosine phosphatases inactivate the osmotic stress response pathway in yeast by targeting the mitogen-activated protein kinase, Hog1."
    Jacoby T., Flanagan H., Faykin A., Seto A.G., Mattison C.P., Ota I.M.
    J. Biol. Chem. 272:17749-17755(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PTP2 AND PTP3, ENZYME REGULATION.
  10. "The Hog1 MAPK prevents cross talk between the HOG and pheromone response MAPK pathways in Saccharomyces cerevisiae."
    O'Rourke S.M., Herskowitz I.
    Genes Dev. 12:2874-2886(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "The high osmolarity glycerol response (HOG) MAP kinase pathway controls localization of a yeast Golgi glycosyltransferase."
    Reynolds T.B., Hopkins B.D., Lyons M.R., Graham T.R.
    J. Cell Biol. 143:935-946(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Kinase activity-dependent nuclear export opposes stress-induced nuclear accumulation and retention of Hog1 mitogen-activated protein kinase in the budding yeast Saccharomyces cerevisiae."
    Reiser V., Ruis H., Ammerer G.
    Mol. Biol. Cell 10:1147-1161(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION.
  13. "Yeast Cdc42 GTPase and Ste20 PAK-like kinase regulate Sho1-dependent activation of the Hog1 MAPK pathway."
    Raitt D.C., Posas F., Saito H.
    EMBO J. 19:4623-4631(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. "Two protein tyrosine phosphatases, Ptp2 and Ptp3, modulate the subcellular localization of the Hog1 MAP kinase in yeast."
    Mattison C.P., Ota I.M.
    Genes Dev. 14:1229-1235(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  15. "Rck2 kinase is a substrate for the osmotic stress-activated mitogen-activated protein kinase Hog1."
    Bilsland-Marchesan E., Arino J., Saito H., Sunnerhagen P., Posas F.
    Mol. Cell. Biol. 20:3887-3895(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RCK2.
  16. "Regulation of the Sko1 transcriptional repressor by the Hog1 MAP kinase in response to osmotic stress."
    Proft M., Pascual-Ahuir A., de Nadal E., Arino J., Serrano R., Posas F.
    EMBO J. 20:1123-1133(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION, INTERACTION WITH SKO1.
  17. "Defects in glycosylphosphatidylinositol (GPI) anchor synthesis activate Hog1 kinase and confer copper-resistance in Saccharomyces cerevisisae."
    Toh-e A., Oguchi T.
    Genes Genet. Syst. 76:393-410(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  18. "Isolation of hyperactive mutants of the MAPK p38/Hog1 that are independent of MAPK kinase activation."
    Bell M., Capone R., Pashtan I., Levitzki A., Engelberg D.
    J. Biol. Chem. 276:25351-25358(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF TYR-68; ASP-170; ALA-314; PHE-318; TRP-320; PHE-322; TRP-332 AND ASN-391.
  19. "Stress-induced map kinase Hog1 is part of transcription activation complexes."
    Alepuz P.M., Jovanovic A., Reiser V., Ammerer G.
    Mol. Cell 7:767-777(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  20. "Ptc1, a type 2C Ser/Thr phosphatase, inactivates the HOG pathway by dephosphorylating the mitogen-activated protein kinase Hog1."
    Warmka J., Hanneman J., Lee J., Amin D., Ota I.M.
    Mol. Cell. Biol. 21:51-60(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
  21. "Low external pH induces HOG1-dependent changes in the organization of the Saccharomyces cerevisiae cell wall."
    Kapteyn J.C., ter Riet B., Vink E., Blad S., De Nobel H., Van Den Ende H., Klis F.M.
    Mol. Microbiol. 39:469-479(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  22. "Heat stress activates the yeast high-osmolarity glycerol mitogen-activated protein kinase pathway, and protein tyrosine phosphatases are essential under heat stress."
    Winkler A., Arkind C., Mattison C.P., Burkholder A., Knoche K., Ota I.M.
    Eukaryot. Cell 1:163-173(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
  23. "Role of Ptc2 type 2C Ser/Thr phosphatase in yeast high-osmolarity glycerol pathway inactivation."
    Young C., Mapes J., Hanneman J., Al-Zarban S., Ota I.M.
    Eukaryot. Cell 1:1032-1040(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
  24. "Transient inhibition of translation initiation by osmotic stress."
    Uesono Y., Toh-e A.
    J. Biol. Chem. 277:13848-13855(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  25. "Hog1 kinase converts the Sko1-Cyc8-Tup1 repressor complex into an activator that recruits SAGA and SWI/SNF in response to osmotic stress."
    Proft M., Struhl K.
    Mol. Cell 9:1307-1317(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  26. "Osmostress-induced transcription by Hot1 depends on a Hog1-mediated recruitment of the RNA Pol II."
    Alepuz P.M., de Nadal E., Zapater M., Ammerer G., Posas F.
    EMBO J. 22:2433-2442(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HOT1; KIN28; RBP1 AND SIN4.
  27. "Phosphorylation of Tyr-176 of the yeast MAPK Hog1/p38 is not vital for Hog1 biological activity."
    Bell M., Engelberg D.
    J. Biol. Chem. 278:14603-14606(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT THR-174 AND TYR-176, MUTAGENESIS OF THR-174 AND TYR-176.
  28. "Targeting the MEF2-like transcription factor Smp1 by the stress-activated Hog1 mitogen-activated protein kinase."
    de Nadal E., Casadome L., Posas F.
    Mol. Cell. Biol. 23:229-237(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SMP1.
  29. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  30. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  31. "Expression of YAP4 in Saccharomyces cerevisiae under osmotic stress."
    Nevitt T., Pereira J., Azevedo D., Guerreiro P., Rodrigues-Pousada C.
    Biochem. J. 379:367-374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  32. "The Hog1 MAP kinase pathway and the Mec1 DNA damage checkpoint pathway independently control the cellular responses to hydrogen peroxide."
    Haghnazari E., Heyer W.-D.
    DNA Repair 3:769-776(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  33. "Evidence of a new role for the high-osmolarity glycerol mitogen-activated protein kinase pathway in yeast: regulating adaptation to citric acid stress."
    Lawrence C.L., Botting C.H., Antrobus R., Coote P.J.
    Mol. Cell. Biol. 24:3307-3323(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION.
  34. "The MAPK Hog1 recruits Rpd3 histone deacetylase to activate osmoresponsive genes."
    De Nadal E., Zapater M., Alepuz P.M., Sumoy L., Mas G., Posas F.
    Nature 427:370-374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RPD3.
  35. "Hog1 mediates cell-cycle arrest in G1 phase by the dual targeting of Sic1."
    Escote X., Zapater M., Clotet J., Posas F.
    Nat. Cell Biol. 6:997-1002(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SIC1.
  36. "Evidence that C-terminal non-kinase domain of Pbs2p has a role in high osmolarity-induced nuclear localization of Hog1p."
    Sharma P., Mondal A.K.
    Biochem. Biophys. Res. Commun. 328:906-913(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION.
  37. "p38 mitogen-activated protein kinase/Hog1p regulates translation of the AU-rich-element-bearing MFA2 transcript."
    Vasudevan S., Garneau N., Tu Khounh D., Peltz S.W.
    Mol. Cell. Biol. 25:9753-9763(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  38. "The HOG MAP kinase pathway is required for the induction of methylglyoxal-responsive genes and determines methylglyoxal resistance in Saccharomyces cerevisiae."
    Aguilera J., Rodriguez-Vargas S., Prieto J.A.
    Mol. Microbiol. 56:228-239(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  39. "In yeast, loss of Hog1 leads to osmosensitivity of autophagy."
    Prick T., Thumm M., Koehrer K., Haeussinger D., Vom Dahl S.
    Biochem. J. 394:153-161(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  40. "Phosphorylation of Hsl1 by Hog1 leads to a G2 arrest essential for cell survival at high osmolarity."
    Clotet J., Escote X., Adrover M.A., Yaakov G., Gari E., Aldea M., de Nadal E., Posas F.
    EMBO J. 25:2338-2346(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  41. "Genome-scale analysis reveals Sst2 as the principal regulator of mating pheromone signaling in the yeast Saccharomyces cerevisiae."
    Chasse S.A., Flanary P., Parnell S.C., Hao N., Cha J.Y., Siderovski D.P., Dohlman H.G.
    Eukaryot. Cell 5:330-346(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION.
  42. "Analysis of mitogen-activated protein kinase signaling specificity in response to hyperosmotic stress: use of an analog-sensitive HOG1 allele."
    Westfall P.J., Thorner J.
    Eukaryot. Cell 5:1215-1228(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-52 AND ASP-144.
  43. "Mitogen-activated protein kinase Hog1 is essential for the response to arsenite in Saccharomyces cerevisiae."
    Sotelo J., Rodriguez-Gabriel M.A.
    Eukaryot. Cell 5:1826-1830(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION.
  44. "A downshift in temperature activates the high osmolarity glycerol (HOG) pathway, which determines freeze tolerance in Saccharomyces cerevisiae."
    Panadero J., Pallotti C., Rodriguez-Vargas S., Randez-Gil F., Prieto J.A.
    J. Biol. Chem. 281:4638-4645(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION.
  45. "Saccharomyces cerevisiae Hog1 protein phosphorylation upon exposure to bacterial endotoxin."
    Marques J.M., Rodrigues R.J., de Magalhaes-Sant'ana A.C., Goncalves T.
    J. Biol. Chem. 281:24687-24694(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, PHOSPHORYLATION, SUBCELLULAR LOCATION.
  46. Cited for: FUNCTION, PHOSPHORYLATION, SUBCELLULAR LOCATION.
  47. "The stress-activated Hog1 kinase is a selective transcriptional elongation factor for genes responding to osmotic stress."
    Proft M., Mas G., de Nadal E., Vendrell A., Noriega N., Struhl K., Posas F.
    Mol. Cell 23:241-250(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RPB1.
  48. "A systems-biology analysis of feedback inhibition in the Sho1 osmotic-stress-response pathway."
    Hao N., Behar M., Parnell S.C., Torres M.P., Borchers C.H., Elston T.C., Dohlman H.G.
    Curr. Biol. 17:659-667(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF LYS-52.
  49. "Cdc37p is required for stress-induced high-osmolarity glycerol and protein kinase C mitogen-activated protein kinase pathway functionality by interaction with Hog1p and Slt2p (Mpk1p)."
    Hawle P., Horst D., Bebelman J.-P., Yang X.X., Siderius M., van der Vies S.M.
    Eukaryot. Cell 6:521-532(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CDC37, PHOSPHORYLATION.
  50. "Dissecting yeast Hog1 MAP kinase pathway using a chemical genetic approach."
    Kim S., Shah K.
    FEBS Lett. 581:1209-1216(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION.
  51. "Plc1p is required for SAGA recruitment and derepression of Sko1p-regulated genes."
    Guha N., Desai P., Vancura A.
    Mol. Biol. Cell 18:2419-2428(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  52. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  53. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  54. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHOG1_YEAST
AccessioniPrimary (citable) accession number: P32485
Secondary accession number(s): D6VYB1, Q06232, Q12294
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: November 1, 1997
Last modified: September 3, 2014
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6780 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3

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