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Protein

Eukaryotic translation initiation factor 2 subunit gamma

Gene

GCD11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Miscellaneous

Present with 20800 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi107 – 114GTPBy similarity8
Nucleotide bindingi193 – 197GTPBy similarity5
Nucleotide bindingi249 – 252GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • translation initiation factor activity Source: SGD
  • translation initiation factor binding Source: SGD

GO - Biological processi

  • formation of translation preinitiation complex Source: SGD
  • positive regulation of translational fidelity Source: SGD
  • translational initiation Source: SGD

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30207-MONOMER
ReactomeiR-SCE-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-SCE-382556 ABC-family proteins mediated transport
R-SCE-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-SCE-72702 Ribosomal scanning and start codon recognition
R-SCE-72731 Recycling of eIF2:GDP

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2 subunit gamma
Short name:
eIF-2-gamma
Gene namesi
Name:GCD11
Synonyms:TIF213
Ordered Locus Names:YER025W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER025W
SGDiS000000827 GCD11

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001374481 – 527Eukaryotic translation initiation factor 2 subunit gammaAdd BLAST527

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei60PhosphothreonineCombined sources1
Modified residuei258PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32481
PaxDbiP32481
PRIDEiP32481

PTM databases

iPTMnetiP32481

Interactioni

Subunit structurei

Eukaryotic translation initiation factor 2 (eIF-2) is a heterotrimer composed of an alpha, a beta and a gamma subunit. The factors eIF-1, eIF-2, eIF-3, TIF5/eIF-5 and methionyl-tRNAi form a multifactor complex (MFC) that may bind to the 40S ribosome.

Binary interactionsi

Show more details

GO - Molecular functioni

  • translation initiation factor binding Source: SGD

Protein-protein interaction databases

BioGridi36759, 429 interactors
DIPiDIP-2561N
IntActiP32481, 78 interactors
MINTiP32481
STRINGi4932.YER025W

Structurei

Secondary structure

1527
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi425 – 436Combined sources12
Beta strandi460 – 466Combined sources7
Beta strandi469 – 478Combined sources10
Beta strandi480 – 493Combined sources14
Beta strandi498 – 504Combined sources7
Beta strandi506 – 523Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J81electron microscopy4.00k1-527[»]
3JAPelectron microscopy4.90k1-527[»]
3JAQelectron microscopy6.00k1-527[»]
4ZGNX-ray2.90B410-527[»]
4ZGQX-ray3.00B410-527[»]
ProteinModelPortaliP32481
SMRiP32481
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini98 – 307tr-type GPROSITE-ProRule annotationAdd BLAST210

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni107 – 114G1PROSITE-ProRule annotation8
Regioni135 – 139G2PROSITE-ProRule annotation5
Regioni193 – 196G3PROSITE-ProRule annotation4
Regioni249 – 252G4PROSITE-ProRule annotation4
Regioni284 – 286G5PROSITE-ProRule annotation3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi155 – 179Cys-richAdd BLAST25

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EIF2G subfamily.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000074801
HOGENOMiHOG000229292
InParanoidiP32481
KOiK03242
OMAiCPQPQTK
OrthoDBiEOG092C1URX

Family and domain databases

CDDicd15490 eIF2_gamma_III, 1 hit
InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR027417 P-loop_NTPase
IPR000795 TF_GTP-bd_dom
IPR015256 TIF2_gsu_C
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
PfamiView protein in Pfam
PF09173 eIF2_C, 1 hit
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P32481-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDLQDQEPS IIINGNLEPV GEPDIVEETE VVAQETQETQ DADKPKKKVA
60 70 80 90 100
FTGLEEDGET EEEKRKREFE EGGGLPEQPL NPDFSKLNPL SAEIINRQAT
110 120 130 140 150
INIGTIGHVA HGKSTVVRAI SGVQTVRFKD ELERNITIKL GYANAKIYKC
160 170 180 190 200
QEPTCPEPDC YRSFKSDKEI SPKCQRPGCP GRYKLVRHVS FVDCPGHDIL
210 220 230 240 250
MSTMLSGAAV MDAALLLIAG NESCPQPQTS EHLAAIEIMK LKHVIILQNK
260 270 280 290 300
VDLMREESAL EHQKSILKFI RGTIADGAPI VPISAQLKYN IDAVNEFIVK
310 320 330 340 350
TIPVPPRDFM ISPRLIVIRS FDVNKPGAEI EDLKGGVAGG SILNGVFKLG
360 370 380 390 400
DEIEIRPGIV TKDDKGKIQC KPIFSNIVSL FAEQNDLKFA VPGGLIGVGT
410 420 430 440 450
KVDPTLCRAD RLVGQVVGAK GHLPNIYTDI EINYFLLRRL LGVKTDGQKQ
460 470 480 490 500
AKVRKLEPNE VLMVNIGSTA TGARVVAVKA DMARLQLTSP ACTEINEKIA
510 520
LSRRIEKHWR LIGWATIKKG TTLEPIA
Length:527
Mass (Da):57,866
Last modified:October 1, 1993 - v1
Checksum:iD498AE62BC3E81CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04268 Genomic DNA Translation: AAA34633.1
U18778 Genomic DNA Translation: AAB64558.1
BK006939 Genomic DNA Translation: DAA07678.1
PIRiA48117
RefSeqiNP_010942.1, NM_001178916.1

Genome annotation databases

EnsemblFungiiYER025W; YER025W; YER025W
GeneIDi856746
KEGGisce:YER025W

Similar proteinsi

Entry informationi

Entry nameiIF2G_YEAST
AccessioniPrimary (citable) accession number: P32481
Secondary accession number(s): D3DLS4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 170 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

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