P32480 (HIR2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein HIR2

Alternative name(s):
Histone transcription regulator 2

Gene names

Name:	HIR2
Ordered Locus Names:	YOR038C
ORF Names:	OR26.31

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	875 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the HIR complex, which cooperates with ASF1 to promote replication-independent chromatin assembly. The HIR complex is also required for the periodic repression of three of the four histone gene loci during the cell cycle as well as for autogenous regulation of the HTA1-HTB1 locus by H2A and H2B. DNA-binding by the HIR complex may repress transcription by inhibiting nucleosome remodeling by the SWI/SNF complex. The HIR complex may also be required for transcriptional silencing of centromeric, telomeric and mating-type loci in the absence of CAF-1. Ref.1 Ref.5 Ref.6 Ref.7 Ref.8 Ref.10 Ref.11 Ref.13 Ref.14 Ref.15
Subunit structure	Component of the HIR complex, composed of HIR1, HIR2, HIR3 and HPC2. This complex may consist of one copy of HIR1 and HIR3 and two copies of HIR2 and HPC2. The HIR complex interacts with ASF1. Interacts with SNF2, SNF5 and SWI3. Ref.5 Ref.8 Ref.9 Ref.13 Ref.14
Subcellular location	Nucleus Ref.1.
Miscellaneous	Present with 922 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the WD repeat HIR1 family. Contains 8 WD repeats.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat WD repeat
Molecular function	Chromatin regulator Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication-independent nucleosome assembly Inferred from direct assay Ref.14Ref.13. Source: SGD chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.5. Source: SGD transcription elongation from RNA polymerase II promoter Inferred from genetic interaction Ref.15. Source: SGD
Cellular_component	HIR complex Inferred from direct assay Ref.14Ref.13. Source: SGD nucleus Inferred from direct assay Ref.1. Source: SGD
Molecular_function	transcription corepressor activity Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HIR1	P32479	5	EBI-8323,EBI-8316

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 875	875	Protein HIR2		PRO_0000051017
Regions
	Repeat	10 – 47	38	WD 1
	Repeat	118 – 158	41	WD 2
	Repeat	163 – 201	39	WD 3
	Repeat	237 – 277	41	WD 4
	Repeat	278 – 316	39	WD 5
	Repeat	320 – 359	40	WD 6
	Repeat	546 – 587	42	WD 7
	Repeat	589 – 626	38	WD 8
Amino acid modifications
	Modified residue	438	1	Phosphoserine Ref.16
	Modified residue	713	1	Phosphoserine Ref.16
Experimental info
	Sequence conflict	284 – 285	2	KG → NF Ref.1
	Sequence conflict	373 – 374	2	KK → FL Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32480 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: C77D677BF424680D	FASTA	875	98,445
	10 20 30 40 50 60 MRLLKYPLDI HNEQVNALAA LGPYIILAGS GGHVMAWRQQ QLVDTAFDRV MIKDLKPEVS 70 80 90 100 110 120 FQVDQDTTGD IFFITGDLET LYIGSEHRLW GYSGWLCRDT NNINSVEKMN SKLLFECKSP 130 140 150 160 170 180 STITDVKYDI NLGILFVLLS NENKILLFRH KTFDKLSEIT IDKASKPITG IIDPTGQTFT 190 200 210 220 230 240 VMTSDRSILV YQINKTGTHK LINKLTQHVQ MYPLHYRISM SPQADILPVI NSVKGVPNNA 250 260 270 280 290 300 TSCTALLDRN NNYKVTKTLV TPSSNGCRVL VYSPAFYEKP NLKKGTSTRY NLIATSGSTD 310 320 330 340 350 360 GTILVWNTKR MKPLFNALQV SSTAINDMSW SQDGFTLFAI SNDATLYTFA FQEKDLGVAL 370 380 390 400 410 420 PQTEIKSLQE VNKKLPKLEE PLAEQIPKSF PENIKLEESA SAAPIPNDIG RSAVGKKPTK 430 440 450 460 470 480 KKTANNQTNG IKTIQSTSME FNTPSYTVPR DLKRKPKEAT PSNIAPGSKK QKKELQPIDF 490 500 510 520 530 540 LDTGLLLPNT SFSRIRLATP KIRSTFKYSP INNPNLILDV KNGSGNEQRP TIVKLTSKVL 550 560 570 580 590 600 DQDQVLFQDF IPKLITICTA GDTFWSFCSE DGSIYIYSDS GRKLMAPLVL GVSISFLEAC 610 620 630 640 650 660 GTYLLCLTSI GELYCWNIEQ KKLAFPTNTI YPLLNPSLRY SDDILTRAEN ITLCSITKKG 670 680 690 700 710 720 VPLVTLSNGD GYLFDKNMET WLLVSDGWWA YGSQYWDTTN TTGLSSSKAN TDSFNGSESN 730 740 750 760 770 780 INEIVSDIKN DNQSIINFLE CKTNDELNRK GRIKNLQRFA RTILMKEGFE NMEEIVTLSH 790 800 810 820 830 840 LENKILISIR LEEPEEFSKL MMVYCIRLSE LGYMDRLNDV FQWLYDDLPI SGTGSAFADK 850 860 870 DFKRNLLKKI LIACGDIRQV QRVTTRYAKE MNIIS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of HIR1 and HIR2, two genes required for regulation of histone gene transcription in Saccharomyces cerevisiae." Sherwood P.W., Tsang S.V., Osley M.A. Mol. Cell. Biol. 13:28-38(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Cloning and disruption of CKB2, the gene encoding the 32-kDa regulatory beta'-subunit of Saccharomyces cerevisiae casein kinase II." Reed J.C., Bidwai A.P., Glover C.V.C. J. Biol. Chem. 269:18192-18200(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-113.
[5]	"Hir1p and Hir2p function as transcriptional corepressors to regulate histone gene transcription in the Saccharomyces cerevisiae cell cycle." Spector M.S., Raff A., DeSilva H., Lee K., Osley M.A. Mol. Cell. Biol. 17:545-552(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH HIR1.
[6]	"Yeast Ty1 retrotransposition is stimulated by a synergistic interaction between mutations in chromatin assembly factor I and histone regulatory proteins." Qian Z., Huang H., Hong J.Y., Burck C.L., Johnston S.D., Berman J., Carol A., Liebman S.W. Mol. Cell. Biol. 18:4783-4792(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Hir proteins are required for position-dependent gene silencing in Saccharomyces cerevisiae in the absence of chromatin assembly factor I." Kaufman P.D., Cohen J.L., Osley M.A. Mol. Cell. Biol. 18:4793-4806(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"A role for transcriptional repressors in targeting the yeast Swi/Snf complex." Dimova D., Nackerdien Z., Furgeson S., Eguchi S., Osley M.A. Mol. Cell 4:75-83(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SNF2; SNF5 AND SWI3.
[9]	"Yeast histone deposition protein Asf1p requires Hir proteins and PCNA for heterochromatic silencing." Sharp J.A., Fouts E.T., Krawitz D.C., Kaufman P.D. Curr. Biol. 11:463-473(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ASF1.
[10]	"Genome-wide location and regulated recruitment of the RSC nucleosome-remodeling complex." Ng H.H., Robert F., Young R.A., Struhl K. Genes Dev. 16:806-819(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[11]	"Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae cause dependence on the Hir/Hpc pathway: polymerase passage may degrade chromatin structure." Formosa T., Ruone S., Adams M.D., Olsen A.E., Eriksson P., Yu Y., Rhoades A.R., Kaufman P.D., Stillman D.J. Genetics 162:1557-1571(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[12]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[13]	"Replication-independent histone deposition by the HIR complex and Asf1." Green E.M., Antczak A.J., Bailey A.O., Franco A.A., Wu K.J., Yates J.R. III, Kaufman P.D. Curr. Biol. 15:2044-2049(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH HIR1; HIR3 AND HPC2, INTERACTION WITH ASF1.
[14]	"The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF." Prochasson P., Florens L., Swanson S.K., Washburn M.P., Workman J.L. Genes Dev. 19:2534-2539(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH HIR1; HIR3 AND HPC2.
[15]	"Evidence that Spt2/Sin1, an HMG-like factor, plays roles in transcription elongation, chromatin structure, and genome stability in Saccharomyces cerevisiae." Nourani A., Robert F., Winston F. Mol. Cell. Biol. 26:1496-1509(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[16]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-438 AND SER-713, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L03839 Genomic DNA. No translation available. X87331 Genomic DNA. Translation: CAA60757.1. Z74946 Genomic DNA. Translation: CAA99228.1. U08849 Genomic DNA. Translation: AAA21657.1. BK006948 Genomic DNA. Translation: DAA10821.1.
PIR	S62177.
RefSeq	NP_014681.1. NM_001183457.1.
3D structure databases
ProteinModelPortal	P32480.
SMR	P32480. Positions 120-356.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2366N.
IntAct	P32480. 20 interactions.
MINT	MINT-688992.
STRING	4932.YOR038C.
Proteomic databases
PaxDb	P32480.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR038C; YOR038C; YOR038C.
GeneID	854203.
KEGG	sce:YOR038C.
Organism-specific databases
CYGD	YOR038c.
SGD	S000005564. HIR2.
Phylogenomic databases
eggNOG	COG2319.
HOGENOM	HOG000112901.
OMA	WAYGSQY.
OrthoDB	EOG4J9R76.
Enzyme and pathway databases
BioCyc	YEAST:G3O-33584-MONOMER.
Gene expression databases
Genevestigator	P32480.
GermOnline	YOR038C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.130.10.10. 1 hit.
InterPro	IPR011494. Hira. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF07569. Hira. 1 hit. [Graphical view]
SMART	SM00320. WD40. 3 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 1 hit. PS50082. WD_REPEATS_2. False negative. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	976044.

Entry information

Entry name

HIR2_YEAST

Accession

Primary (citable) accession number: P32480
Secondary accession number(s): D6W2A5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 124 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program