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Protein

Protein HIR2

Gene

HIR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the HIR complex, which cooperates with ASF1 to promote replication-independent chromatin assembly. The HIR complex is also required for the periodic repression of three of the four histone gene loci during the cell cycle as well as for autogenous regulation of the HTA1-HTB1 locus by H2A and H2B. DNA-binding by the HIR complex may repress transcription by inhibiting nucleosome remodeling by the SWI/SNF complex. The HIR complex may also be required for transcriptional silencing of centromeric, telomeric and mating-type loci in the absence of CAF-1.10 Publications

Miscellaneous

Present with 922 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • transcription corepressor activity Source: SGD

GO - Biological processi

  • DNA replication-independent nucleosome assembly Source: SGD
  • negative regulation of transcription by RNA polymerase II Source: SGD
  • regulation of chromatin silencing Source: GO_Central
  • transcription elongation from RNA polymerase II promoter Source: SGD

Keywordsi

Molecular functionChromatin regulator, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-33584-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Protein HIR2
Alternative name(s):
Histone transcription regulator 2
Gene namesi
Name:HIR2
Ordered Locus Names:YOR038C
ORF Names:OR26.31
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR038C
SGDiS000005564 HIR2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000510171 – 875Protein HIR2Add BLAST875

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei713PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32480
PaxDbiP32480
PRIDEiP32480

PTM databases

iPTMnetiP32480

Interactioni

Subunit structurei

Component of the HIR complex, composed of HIR1, HIR2, HIR3 and HPC2. This complex may consist of one copy of HIR1 and HIR3 and two copies of HIR2 and HPC2. The HIR complex interacts with ASF1. Interacts with SNF2, SNF5 and SWI3.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HIR1P324798EBI-8323,EBI-8316

Protein-protein interaction databases

BioGridi34440, 420 interactors
DIPiDIP-2366N
IntActiP32480, 23 interactors
MINTiP32480
STRINGi4932.YOR038C

Structurei

3D structure databases

ProteinModelPortaliP32480
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati10 – 47WD 1Add BLAST38
Repeati118 – 158WD 2Add BLAST41
Repeati163 – 201WD 3Add BLAST39
Repeati237 – 277WD 4Add BLAST41
Repeati278 – 316WD 5Add BLAST39
Repeati320 – 359WD 6Add BLAST40
Repeati546 – 587WD 7Add BLAST42
Repeati589 – 626WD 8Add BLAST38

Sequence similaritiesi

Belongs to the WD repeat HIR1 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

HOGENOMiHOG000112901
InParanoidiP32480
KOiK11293
OrthoDBiEOG092C0UNU

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR031120 HIR1
IPR011494 Hira
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR13831 PTHR13831, 1 hit
PfamiView protein in Pfam
PF07569 Hira, 1 hit
SUPFAMiSSF50978 SSF50978, 2 hits
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

P32480-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLLKYPLDI HNEQVNALAA LGPYIILAGS GGHVMAWRQQ QLVDTAFDRV
60 70 80 90 100
MIKDLKPEVS FQVDQDTTGD IFFITGDLET LYIGSEHRLW GYSGWLCRDT
110 120 130 140 150
NNINSVEKMN SKLLFECKSP STITDVKYDI NLGILFVLLS NENKILLFRH
160 170 180 190 200
KTFDKLSEIT IDKASKPITG IIDPTGQTFT VMTSDRSILV YQINKTGTHK
210 220 230 240 250
LINKLTQHVQ MYPLHYRISM SPQADILPVI NSVKGVPNNA TSCTALLDRN
260 270 280 290 300
NNYKVTKTLV TPSSNGCRVL VYSPAFYEKP NLKKGTSTRY NLIATSGSTD
310 320 330 340 350
GTILVWNTKR MKPLFNALQV SSTAINDMSW SQDGFTLFAI SNDATLYTFA
360 370 380 390 400
FQEKDLGVAL PQTEIKSLQE VNKKLPKLEE PLAEQIPKSF PENIKLEESA
410 420 430 440 450
SAAPIPNDIG RSAVGKKPTK KKTANNQTNG IKTIQSTSME FNTPSYTVPR
460 470 480 490 500
DLKRKPKEAT PSNIAPGSKK QKKELQPIDF LDTGLLLPNT SFSRIRLATP
510 520 530 540 550
KIRSTFKYSP INNPNLILDV KNGSGNEQRP TIVKLTSKVL DQDQVLFQDF
560 570 580 590 600
IPKLITICTA GDTFWSFCSE DGSIYIYSDS GRKLMAPLVL GVSISFLEAC
610 620 630 640 650
GTYLLCLTSI GELYCWNIEQ KKLAFPTNTI YPLLNPSLRY SDDILTRAEN
660 670 680 690 700
ITLCSITKKG VPLVTLSNGD GYLFDKNMET WLLVSDGWWA YGSQYWDTTN
710 720 730 740 750
TTGLSSSKAN TDSFNGSESN INEIVSDIKN DNQSIINFLE CKTNDELNRK
760 770 780 790 800
GRIKNLQRFA RTILMKEGFE NMEEIVTLSH LENKILISIR LEEPEEFSKL
810 820 830 840 850
MMVYCIRLSE LGYMDRLNDV FQWLYDDLPI SGTGSAFADK DFKRNLLKKI
860 870
LIACGDIRQV QRVTTRYAKE MNIIS
Length:875
Mass (Da):98,445
Last modified:October 1, 1996 - v2
Checksum:iC77D677BF424680D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti284 – 285KG → NF (PubMed:8417331).Curated2
Sequence conflicti373 – 374KK → FL (PubMed:8417331).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03839 Genomic DNA No translation available.
X87331 Genomic DNA Translation: CAA60757.1
Z74946 Genomic DNA Translation: CAA99228.1
U08849 Genomic DNA Translation: AAA21657.1
BK006948 Genomic DNA Translation: DAA10821.1
PIRiS62177
RefSeqiNP_014681.1, NM_001183457.1

Genome annotation databases

EnsemblFungiiYOR038C; YOR038C; YOR038C
GeneIDi854203
KEGGisce:YOR038C

Similar proteinsi

Entry informationi

Entry nameiHIR2_YEAST
AccessioniPrimary (citable) accession number: P32480
Secondary accession number(s): D6W2A5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 166 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

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