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P32480 (HIR2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein HIR2
Alternative name(s):
Histone transcription regulator 2
Gene names
Name:HIR2
Ordered Locus Names:YOR038C
ORF Names:OR26.31
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length875 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the HIR complex, which cooperates with ASF1 to promote replication-independent chromatin assembly. The HIR complex is also required for the periodic repression of three of the four histone gene loci during the cell cycle as well as for autogenous regulation of the HTA1-HTB1 locus by H2A and H2B. DNA-binding by the HIR complex may repress transcription by inhibiting nucleosome remodeling by the SWI/SNF complex. The HIR complex may also be required for transcriptional silencing of centromeric, telomeric and mating-type loci in the absence of CAF-1. Ref.1 Ref.5 Ref.6 Ref.7 Ref.8 Ref.10 Ref.11 Ref.13 Ref.14 Ref.15

Subunit structure

Component of the HIR complex, composed of HIR1, HIR2, HIR3 and HPC2. This complex may consist of one copy of HIR1 and HIR3 and two copies of HIR2 and HPC2. The HIR complex interacts with ASF1. Interacts with SNF2, SNF5 and SWI3. Ref.5 Ref.8 Ref.9 Ref.13 Ref.14

Subcellular location

Nucleus Ref.1.

Miscellaneous

Present with 922 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the WD repeat HIR1 family.

Contains 8 WD repeats.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

HIR1P324795EBI-8323,EBI-8316

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 875875Protein HIR2
PRO_0000051017

Regions

Repeat10 – 4738WD 1
Repeat118 – 15841WD 2
Repeat163 – 20139WD 3
Repeat237 – 27741WD 4
Repeat278 – 31639WD 5
Repeat320 – 35940WD 6
Repeat546 – 58742WD 7
Repeat589 – 62638WD 8

Amino acid modifications

Modified residue7131Phosphoserine Ref.16

Experimental info

Sequence conflict284 – 2852KG → NF Ref.1
Sequence conflict373 – 3742KK → FL Ref.1

Sequences

Sequence LengthMass (Da)Tools
P32480 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: C77D677BF424680D

FASTA87598,445
        10         20         30         40         50         60 
MRLLKYPLDI HNEQVNALAA LGPYIILAGS GGHVMAWRQQ QLVDTAFDRV MIKDLKPEVS 

        70         80         90        100        110        120 
FQVDQDTTGD IFFITGDLET LYIGSEHRLW GYSGWLCRDT NNINSVEKMN SKLLFECKSP 

       130        140        150        160        170        180 
STITDVKYDI NLGILFVLLS NENKILLFRH KTFDKLSEIT IDKASKPITG IIDPTGQTFT 

       190        200        210        220        230        240 
VMTSDRSILV YQINKTGTHK LINKLTQHVQ MYPLHYRISM SPQADILPVI NSVKGVPNNA 

       250        260        270        280        290        300 
TSCTALLDRN NNYKVTKTLV TPSSNGCRVL VYSPAFYEKP NLKKGTSTRY NLIATSGSTD 

       310        320        330        340        350        360 
GTILVWNTKR MKPLFNALQV SSTAINDMSW SQDGFTLFAI SNDATLYTFA FQEKDLGVAL 

       370        380        390        400        410        420 
PQTEIKSLQE VNKKLPKLEE PLAEQIPKSF PENIKLEESA SAAPIPNDIG RSAVGKKPTK 

       430        440        450        460        470        480 
KKTANNQTNG IKTIQSTSME FNTPSYTVPR DLKRKPKEAT PSNIAPGSKK QKKELQPIDF 

       490        500        510        520        530        540 
LDTGLLLPNT SFSRIRLATP KIRSTFKYSP INNPNLILDV KNGSGNEQRP TIVKLTSKVL 

       550        560        570        580        590        600 
DQDQVLFQDF IPKLITICTA GDTFWSFCSE DGSIYIYSDS GRKLMAPLVL GVSISFLEAC 

       610        620        630        640        650        660 
GTYLLCLTSI GELYCWNIEQ KKLAFPTNTI YPLLNPSLRY SDDILTRAEN ITLCSITKKG 

       670        680        690        700        710        720 
VPLVTLSNGD GYLFDKNMET WLLVSDGWWA YGSQYWDTTN TTGLSSSKAN TDSFNGSESN 

       730        740        750        760        770        780 
INEIVSDIKN DNQSIINFLE CKTNDELNRK GRIKNLQRFA RTILMKEGFE NMEEIVTLSH 

       790        800        810        820        830        840 
LENKILISIR LEEPEEFSKL MMVYCIRLSE LGYMDRLNDV FQWLYDDLPI SGTGSAFADK 

       850        860        870 
DFKRNLLKKI LIACGDIRQV QRVTTRYAKE MNIIS 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of HIR1 and HIR2, two genes required for regulation of histone gene transcription in Saccharomyces cerevisiae."
Sherwood P.W., Tsang S.V., Osley M.A.
Mol. Cell. Biol. 13:28-38(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Cloning and disruption of CKB2, the gene encoding the 32-kDa regulatory beta'-subunit of Saccharomyces cerevisiae casein kinase II."
Reed J.C., Bidwai A.P., Glover C.V.C.
J. Biol. Chem. 269:18192-18200(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-113.
[5]"Hir1p and Hir2p function as transcriptional corepressors to regulate histone gene transcription in the Saccharomyces cerevisiae cell cycle."
Spector M.S., Raff A., DeSilva H., Lee K., Osley M.A.
Mol. Cell. Biol. 17:545-552(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH HIR1.
[6]"Yeast Ty1 retrotransposition is stimulated by a synergistic interaction between mutations in chromatin assembly factor I and histone regulatory proteins."
Qian Z., Huang H., Hong J.Y., Burck C.L., Johnston S.D., Berman J., Carol A., Liebman S.W.
Mol. Cell. Biol. 18:4783-4792(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Hir proteins are required for position-dependent gene silencing in Saccharomyces cerevisiae in the absence of chromatin assembly factor I."
Kaufman P.D., Cohen J.L., Osley M.A.
Mol. Cell. Biol. 18:4793-4806(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"A role for transcriptional repressors in targeting the yeast Swi/Snf complex."
Dimova D., Nackerdien Z., Furgeson S., Eguchi S., Osley M.A.
Mol. Cell 4:75-83(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SNF2; SNF5 AND SWI3.
[9]"Yeast histone deposition protein Asf1p requires Hir proteins and PCNA for heterochromatic silencing."
Sharp J.A., Fouts E.T., Krawitz D.C., Kaufman P.D.
Curr. Biol. 11:463-473(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ASF1.
[10]"Genome-wide location and regulated recruitment of the RSC nucleosome-remodeling complex."
Ng H.H., Robert F., Young R.A., Struhl K.
Genes Dev. 16:806-819(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae cause dependence on the Hir/Hpc pathway: polymerase passage may degrade chromatin structure."
Formosa T., Ruone S., Adams M.D., Olsen A.E., Eriksson P., Yu Y., Rhoades A.R., Kaufman P.D., Stillman D.J.
Genetics 162:1557-1571(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[13]"Replication-independent histone deposition by the HIR complex and Asf1."
Green E.M., Antczak A.J., Bailey A.O., Franco A.A., Wu K.J., Yates J.R. III, Kaufman P.D.
Curr. Biol. 15:2044-2049(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH HIR1; HIR3 AND HPC2, INTERACTION WITH ASF1.
[14]"The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF."
Prochasson P., Florens L., Swanson S.K., Washburn M.P., Workman J.L.
Genes Dev. 19:2534-2539(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH HIR1; HIR3 AND HPC2.
[15]"Evidence that Spt2/Sin1, an HMG-like factor, plays roles in transcription elongation, chromatin structure, and genome stability in Saccharomyces cerevisiae."
Nourani A., Robert F., Winston F.
Mol. Cell. Biol. 26:1496-1509(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[16]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-713, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L03839 Genomic DNA. No translation available.
X87331 Genomic DNA. Translation: CAA60757.1.
Z74946 Genomic DNA. Translation: CAA99228.1.
U08849 Genomic DNA. Translation: AAA21657.1.
BK006948 Genomic DNA. Translation: DAA10821.1.
PIRS62177.
RefSeqNP_014681.1. NM_001183457.1.

3D structure databases

ProteinModelPortalP32480.
SMRP32480. Positions 160-356.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34440. 243 interactions.
DIPDIP-2366N.
IntActP32480. 19 interactions.
MINTMINT-688992.
STRING4932.YOR038C.

Proteomic databases

PaxDbP32480.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR038C; YOR038C; YOR038C.
GeneID854203.
KEGGsce:YOR038C.

Organism-specific databases

CYGDYOR038c.
SGDS000005564. HIR2.

Phylogenomic databases

eggNOGCOG2319.
HOGENOMHOG000112901.
KOK11293.
OMAHLENKIL.
OrthoDBEOG7NCVC9.

Enzyme and pathway databases

BioCycYEAST:G3O-33584-MONOMER.

Gene expression databases

GenevestigatorP32480.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR011494. Hira.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF07569. Hira. 1 hit.
[Graphical view]
SMARTSM00320. WD40. 3 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 2 hits.
PROSITEPS00678. WD_REPEATS_1. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio976044.

Entry information

Entry nameHIR2_YEAST
AccessionPrimary (citable) accession number: P32480
Secondary accession number(s): D6W2A5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: March 19, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families