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Protein

Protein HIR2

Gene

HIR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the HIR complex, which cooperates with ASF1 to promote replication-independent chromatin assembly. The HIR complex is also required for the periodic repression of three of the four histone gene loci during the cell cycle as well as for autogenous regulation of the HTA1-HTB1 locus by H2A and H2B. DNA-binding by the HIR complex may repress transcription by inhibiting nucleosome remodeling by the SWI/SNF complex. The HIR complex may also be required for transcriptional silencing of centromeric, telomeric and mating-type loci in the absence of CAF-1.10 Publications

GO - Molecular functioni

  • transcription corepressor activity Source: SGD

GO - Biological processi

  • chromatin modification Source: UniProtKB-KW
  • DNA replication-independent nucleosome assembly Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • transcription elongation from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-33584-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein HIR2
Alternative name(s):
Histone transcription regulator 2
Gene namesi
Name:HIR2
Ordered Locus Names:YOR038C
ORF Names:OR26.31
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XV

Organism-specific databases

CYGDiYOR038c.
EuPathDBiFungiDB:YOR038C.
SGDiS000005564. HIR2.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • cytosol Source: SGD
  • HIR complex Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 875875Protein HIR2PRO_0000051017Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei713 – 7131Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32480.
PaxDbiP32480.

Interactioni

Subunit structurei

Component of the HIR complex, composed of HIR1, HIR2, HIR3 and HPC2. This complex may consist of one copy of HIR1 and HIR3 and two copies of HIR2 and HPC2. The HIR complex interacts with ASF1. Interacts with SNF2, SNF5 and SWI3.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HIR1P324795EBI-8323,EBI-8316

Protein-protein interaction databases

BioGridi34440. 243 interactions.
DIPiDIP-2366N.
IntActiP32480. 19 interactions.
MINTiMINT-688992.

Structurei

3D structure databases

ProteinModelPortaliP32480.
SMRiP32480. Positions 293-353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati10 – 4738WD 1Add
BLAST
Repeati118 – 15841WD 2Add
BLAST
Repeati163 – 20139WD 3Add
BLAST
Repeati237 – 27741WD 4Add
BLAST
Repeati278 – 31639WD 5Add
BLAST
Repeati320 – 35940WD 6Add
BLAST
Repeati546 – 58742WD 7Add
BLAST
Repeati589 – 62638WD 8Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat HIR1 family.Curated
Contains 8 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
HOGENOMiHOG000112901.
InParanoidiP32480.
KOiK11293.
OMAiKLITICT.
OrthoDBiEOG7NCVC9.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR031120. HIR1.
IPR011494. Hira.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR13831. PTHR13831. 1 hit.
PfamiPF07569. Hira. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32480-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLLKYPLDI HNEQVNALAA LGPYIILAGS GGHVMAWRQQ QLVDTAFDRV
60 70 80 90 100
MIKDLKPEVS FQVDQDTTGD IFFITGDLET LYIGSEHRLW GYSGWLCRDT
110 120 130 140 150
NNINSVEKMN SKLLFECKSP STITDVKYDI NLGILFVLLS NENKILLFRH
160 170 180 190 200
KTFDKLSEIT IDKASKPITG IIDPTGQTFT VMTSDRSILV YQINKTGTHK
210 220 230 240 250
LINKLTQHVQ MYPLHYRISM SPQADILPVI NSVKGVPNNA TSCTALLDRN
260 270 280 290 300
NNYKVTKTLV TPSSNGCRVL VYSPAFYEKP NLKKGTSTRY NLIATSGSTD
310 320 330 340 350
GTILVWNTKR MKPLFNALQV SSTAINDMSW SQDGFTLFAI SNDATLYTFA
360 370 380 390 400
FQEKDLGVAL PQTEIKSLQE VNKKLPKLEE PLAEQIPKSF PENIKLEESA
410 420 430 440 450
SAAPIPNDIG RSAVGKKPTK KKTANNQTNG IKTIQSTSME FNTPSYTVPR
460 470 480 490 500
DLKRKPKEAT PSNIAPGSKK QKKELQPIDF LDTGLLLPNT SFSRIRLATP
510 520 530 540 550
KIRSTFKYSP INNPNLILDV KNGSGNEQRP TIVKLTSKVL DQDQVLFQDF
560 570 580 590 600
IPKLITICTA GDTFWSFCSE DGSIYIYSDS GRKLMAPLVL GVSISFLEAC
610 620 630 640 650
GTYLLCLTSI GELYCWNIEQ KKLAFPTNTI YPLLNPSLRY SDDILTRAEN
660 670 680 690 700
ITLCSITKKG VPLVTLSNGD GYLFDKNMET WLLVSDGWWA YGSQYWDTTN
710 720 730 740 750
TTGLSSSKAN TDSFNGSESN INEIVSDIKN DNQSIINFLE CKTNDELNRK
760 770 780 790 800
GRIKNLQRFA RTILMKEGFE NMEEIVTLSH LENKILISIR LEEPEEFSKL
810 820 830 840 850
MMVYCIRLSE LGYMDRLNDV FQWLYDDLPI SGTGSAFADK DFKRNLLKKI
860 870
LIACGDIRQV QRVTTRYAKE MNIIS
Length:875
Mass (Da):98,445
Last modified:October 1, 1996 - v2
Checksum:iC77D677BF424680D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti284 – 2852KG → NF (PubMed:8417331).Curated
Sequence conflicti373 – 3742KK → FL (PubMed:8417331).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03839 Genomic DNA. No translation available.
X87331 Genomic DNA. Translation: CAA60757.1.
Z74946 Genomic DNA. Translation: CAA99228.1.
U08849 Genomic DNA. Translation: AAA21657.1.
BK006948 Genomic DNA. Translation: DAA10821.1.
PIRiS62177.
RefSeqiNP_014681.1. NM_001183457.1.

Genome annotation databases

EnsemblFungiiYOR038C; YOR038C; YOR038C.
GeneIDi854203.
KEGGisce:YOR038C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03839 Genomic DNA. No translation available.
X87331 Genomic DNA. Translation: CAA60757.1.
Z74946 Genomic DNA. Translation: CAA99228.1.
U08849 Genomic DNA. Translation: AAA21657.1.
BK006948 Genomic DNA. Translation: DAA10821.1.
PIRiS62177.
RefSeqiNP_014681.1. NM_001183457.1.

3D structure databases

ProteinModelPortaliP32480.
SMRiP32480. Positions 293-353.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34440. 243 interactions.
DIPiDIP-2366N.
IntActiP32480. 19 interactions.
MINTiMINT-688992.

Proteomic databases

MaxQBiP32480.
PaxDbiP32480.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR038C; YOR038C; YOR038C.
GeneIDi854203.
KEGGisce:YOR038C.

Organism-specific databases

CYGDiYOR038c.
EuPathDBiFungiDB:YOR038C.
SGDiS000005564. HIR2.

Phylogenomic databases

eggNOGiCOG2319.
HOGENOMiHOG000112901.
InParanoidiP32480.
KOiK11293.
OMAiKLITICT.
OrthoDBiEOG7NCVC9.

Enzyme and pathway databases

BioCyciYEAST:G3O-33584-MONOMER.

Miscellaneous databases

NextBioi976044.
PROiP32480.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR031120. HIR1.
IPR011494. Hira.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR13831. PTHR13831. 1 hit.
PfamiPF07569. Hira. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of HIR1 and HIR2, two genes required for regulation of histone gene transcription in Saccharomyces cerevisiae."
    Sherwood P.W., Tsang S.V., Osley M.A.
    Mol. Cell. Biol. 13:28-38(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Cloning and disruption of CKB2, the gene encoding the 32-kDa regulatory beta'-subunit of Saccharomyces cerevisiae casein kinase II."
    Reed J.C., Bidwai A.P., Glover C.V.C.
    J. Biol. Chem. 269:18192-18200(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-113.
  5. "Hir1p and Hir2p function as transcriptional corepressors to regulate histone gene transcription in the Saccharomyces cerevisiae cell cycle."
    Spector M.S., Raff A., DeSilva H., Lee K., Osley M.A.
    Mol. Cell. Biol. 17:545-552(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HIR1.
  6. "Yeast Ty1 retrotransposition is stimulated by a synergistic interaction between mutations in chromatin assembly factor I and histone regulatory proteins."
    Qian Z., Huang H., Hong J.Y., Burck C.L., Johnston S.D., Berman J., Carol A., Liebman S.W.
    Mol. Cell. Biol. 18:4783-4792(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Hir proteins are required for position-dependent gene silencing in Saccharomyces cerevisiae in the absence of chromatin assembly factor I."
    Kaufman P.D., Cohen J.L., Osley M.A.
    Mol. Cell. Biol. 18:4793-4806(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "A role for transcriptional repressors in targeting the yeast Swi/Snf complex."
    Dimova D., Nackerdien Z., Furgeson S., Eguchi S., Osley M.A.
    Mol. Cell 4:75-83(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SNF2; SNF5 AND SWI3.
  9. "Yeast histone deposition protein Asf1p requires Hir proteins and PCNA for heterochromatic silencing."
    Sharp J.A., Fouts E.T., Krawitz D.C., Kaufman P.D.
    Curr. Biol. 11:463-473(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ASF1.
  10. "Genome-wide location and regulated recruitment of the RSC nucleosome-remodeling complex."
    Ng H.H., Robert F., Young R.A., Struhl K.
    Genes Dev. 16:806-819(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae cause dependence on the Hir/Hpc pathway: polymerase passage may degrade chromatin structure."
    Formosa T., Ruone S., Adams M.D., Olsen A.E., Eriksson P., Yu Y., Rhoades A.R., Kaufman P.D., Stillman D.J.
    Genetics 162:1557-1571(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  13. "Replication-independent histone deposition by the HIR complex and Asf1."
    Green E.M., Antczak A.J., Bailey A.O., Franco A.A., Wu K.J., Yates J.R. III, Kaufman P.D.
    Curr. Biol. 15:2044-2049(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH HIR1; HIR3 AND HPC2, INTERACTION WITH ASF1.
  14. "The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF."
    Prochasson P., Florens L., Swanson S.K., Washburn M.P., Workman J.L.
    Genes Dev. 19:2534-2539(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH HIR1; HIR3 AND HPC2.
  15. "Evidence that Spt2/Sin1, an HMG-like factor, plays roles in transcription elongation, chromatin structure, and genome stability in Saccharomyces cerevisiae."
    Nourani A., Robert F., Winston F.
    Mol. Cell. Biol. 26:1496-1509(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  16. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-713, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHIR2_YEAST
AccessioniPrimary (citable) accession number: P32480
Secondary accession number(s): D6W2A5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: July 22, 2015
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 922 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.