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P32480

- HIR2_YEAST

UniProt

P32480 - HIR2_YEAST

Protein

Protein HIR2

Gene

HIR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Component of the HIR complex, which cooperates with ASF1 to promote replication-independent chromatin assembly. The HIR complex is also required for the periodic repression of three of the four histone gene loci during the cell cycle as well as for autogenous regulation of the HTA1-HTB1 locus by H2A and H2B. DNA-binding by the HIR complex may repress transcription by inhibiting nucleosome remodeling by the SWI/SNF complex. The HIR complex may also be required for transcriptional silencing of centromeric, telomeric and mating-type loci in the absence of CAF-1.10 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. transcription corepressor activity Source: SGD

    GO - Biological processi

    1. chromatin modification Source: UniProtKB-KW
    2. DNA replication-independent nucleosome assembly Source: SGD
    3. regulation of transcription from RNA polymerase II promoter Source: SGD
    4. transcription elongation from RNA polymerase II promoter Source: SGD

    Keywords - Molecular functioni

    Chromatin regulator, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33584-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein HIR2
    Alternative name(s):
    Histone transcription regulator 2
    Gene namesi
    Name:HIR2
    Ordered Locus Names:YOR038C
    ORF Names:OR26.31
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR038c.
    SGDiS000005564. HIR2.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. HIR complex Source: SGD
    3. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 875875Protein HIR2PRO_0000051017Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei713 – 7131Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP32480.
    PaxDbiP32480.

    Expressioni

    Gene expression databases

    GenevestigatoriP32480.

    Interactioni

    Subunit structurei

    Component of the HIR complex, composed of HIR1, HIR2, HIR3 and HPC2. This complex may consist of one copy of HIR1 and HIR3 and two copies of HIR2 and HPC2. The HIR complex interacts with ASF1. Interacts with SNF2, SNF5 and SWI3.5 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HIR1P324795EBI-8323,EBI-8316

    Protein-protein interaction databases

    BioGridi34440. 244 interactions.
    DIPiDIP-2366N.
    IntActiP32480. 19 interactions.
    MINTiMINT-688992.
    STRINGi4932.YOR038C.

    Structurei

    3D structure databases

    ProteinModelPortaliP32480.
    SMRiP32480. Positions 295-350, 554-579.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati10 – 4738WD 1Add
    BLAST
    Repeati118 – 15841WD 2Add
    BLAST
    Repeati163 – 20139WD 3Add
    BLAST
    Repeati237 – 27741WD 4Add
    BLAST
    Repeati278 – 31639WD 5Add
    BLAST
    Repeati320 – 35940WD 6Add
    BLAST
    Repeati546 – 58742WD 7Add
    BLAST
    Repeati589 – 62638WD 8Add
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat HIR1 family.Curated
    Contains 8 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    HOGENOMiHOG000112901.
    KOiK11293.
    OMAiIRQVQRV.
    OrthoDBiEOG7NCVC9.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR011494. Hira.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF07569. Hira. 1 hit.
    [Graphical view]
    SMARTiSM00320. WD40. 3 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 2 hits.
    PROSITEiPS00678. WD_REPEATS_1. 1 hit.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32480-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRLLKYPLDI HNEQVNALAA LGPYIILAGS GGHVMAWRQQ QLVDTAFDRV    50
    MIKDLKPEVS FQVDQDTTGD IFFITGDLET LYIGSEHRLW GYSGWLCRDT 100
    NNINSVEKMN SKLLFECKSP STITDVKYDI NLGILFVLLS NENKILLFRH 150
    KTFDKLSEIT IDKASKPITG IIDPTGQTFT VMTSDRSILV YQINKTGTHK 200
    LINKLTQHVQ MYPLHYRISM SPQADILPVI NSVKGVPNNA TSCTALLDRN 250
    NNYKVTKTLV TPSSNGCRVL VYSPAFYEKP NLKKGTSTRY NLIATSGSTD 300
    GTILVWNTKR MKPLFNALQV SSTAINDMSW SQDGFTLFAI SNDATLYTFA 350
    FQEKDLGVAL PQTEIKSLQE VNKKLPKLEE PLAEQIPKSF PENIKLEESA 400
    SAAPIPNDIG RSAVGKKPTK KKTANNQTNG IKTIQSTSME FNTPSYTVPR 450
    DLKRKPKEAT PSNIAPGSKK QKKELQPIDF LDTGLLLPNT SFSRIRLATP 500
    KIRSTFKYSP INNPNLILDV KNGSGNEQRP TIVKLTSKVL DQDQVLFQDF 550
    IPKLITICTA GDTFWSFCSE DGSIYIYSDS GRKLMAPLVL GVSISFLEAC 600
    GTYLLCLTSI GELYCWNIEQ KKLAFPTNTI YPLLNPSLRY SDDILTRAEN 650
    ITLCSITKKG VPLVTLSNGD GYLFDKNMET WLLVSDGWWA YGSQYWDTTN 700
    TTGLSSSKAN TDSFNGSESN INEIVSDIKN DNQSIINFLE CKTNDELNRK 750
    GRIKNLQRFA RTILMKEGFE NMEEIVTLSH LENKILISIR LEEPEEFSKL 800
    MMVYCIRLSE LGYMDRLNDV FQWLYDDLPI SGTGSAFADK DFKRNLLKKI 850
    LIACGDIRQV QRVTTRYAKE MNIIS 875
    Length:875
    Mass (Da):98,445
    Last modified:October 1, 1996 - v2
    Checksum:iC77D677BF424680D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti284 – 2852KG → NF(PubMed:8417331)Curated
    Sequence conflicti373 – 3742KK → FL(PubMed:8417331)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L03839 Genomic DNA. No translation available.
    X87331 Genomic DNA. Translation: CAA60757.1.
    Z74946 Genomic DNA. Translation: CAA99228.1.
    U08849 Genomic DNA. Translation: AAA21657.1.
    BK006948 Genomic DNA. Translation: DAA10821.1.
    PIRiS62177.
    RefSeqiNP_014681.1. NM_001183457.1.

    Genome annotation databases

    EnsemblFungiiYOR038C; YOR038C; YOR038C.
    GeneIDi854203.
    KEGGisce:YOR038C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L03839 Genomic DNA. No translation available.
    X87331 Genomic DNA. Translation: CAA60757.1 .
    Z74946 Genomic DNA. Translation: CAA99228.1 .
    U08849 Genomic DNA. Translation: AAA21657.1 .
    BK006948 Genomic DNA. Translation: DAA10821.1 .
    PIRi S62177.
    RefSeqi NP_014681.1. NM_001183457.1.

    3D structure databases

    ProteinModelPortali P32480.
    SMRi P32480. Positions 295-350, 554-579.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34440. 244 interactions.
    DIPi DIP-2366N.
    IntActi P32480. 19 interactions.
    MINTi MINT-688992.
    STRINGi 4932.YOR038C.

    Proteomic databases

    MaxQBi P32480.
    PaxDbi P32480.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR038C ; YOR038C ; YOR038C .
    GeneIDi 854203.
    KEGGi sce:YOR038C.

    Organism-specific databases

    CYGDi YOR038c.
    SGDi S000005564. HIR2.

    Phylogenomic databases

    eggNOGi COG2319.
    HOGENOMi HOG000112901.
    KOi K11293.
    OMAi IRQVQRV.
    OrthoDBi EOG7NCVC9.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33584-MONOMER.

    Miscellaneous databases

    NextBioi 976044.

    Gene expression databases

    Genevestigatori P32480.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR011494. Hira.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF07569. Hira. 1 hit.
    [Graphical view ]
    SMARTi SM00320. WD40. 3 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 2 hits.
    PROSITEi PS00678. WD_REPEATS_1. 1 hit.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of HIR1 and HIR2, two genes required for regulation of histone gene transcription in Saccharomyces cerevisiae."
      Sherwood P.W., Tsang S.V., Osley M.A.
      Mol. Cell. Biol. 13:28-38(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Cloning and disruption of CKB2, the gene encoding the 32-kDa regulatory beta'-subunit of Saccharomyces cerevisiae casein kinase II."
      Reed J.C., Bidwai A.P., Glover C.V.C.
      J. Biol. Chem. 269:18192-18200(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-113.
    5. "Hir1p and Hir2p function as transcriptional corepressors to regulate histone gene transcription in the Saccharomyces cerevisiae cell cycle."
      Spector M.S., Raff A., DeSilva H., Lee K., Osley M.A.
      Mol. Cell. Biol. 17:545-552(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HIR1.
    6. "Yeast Ty1 retrotransposition is stimulated by a synergistic interaction between mutations in chromatin assembly factor I and histone regulatory proteins."
      Qian Z., Huang H., Hong J.Y., Burck C.L., Johnston S.D., Berman J., Carol A., Liebman S.W.
      Mol. Cell. Biol. 18:4783-4792(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Hir proteins are required for position-dependent gene silencing in Saccharomyces cerevisiae in the absence of chromatin assembly factor I."
      Kaufman P.D., Cohen J.L., Osley M.A.
      Mol. Cell. Biol. 18:4793-4806(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "A role for transcriptional repressors in targeting the yeast Swi/Snf complex."
      Dimova D., Nackerdien Z., Furgeson S., Eguchi S., Osley M.A.
      Mol. Cell 4:75-83(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SNF2; SNF5 AND SWI3.
    9. "Yeast histone deposition protein Asf1p requires Hir proteins and PCNA for heterochromatic silencing."
      Sharp J.A., Fouts E.T., Krawitz D.C., Kaufman P.D.
      Curr. Biol. 11:463-473(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ASF1.
    10. "Genome-wide location and regulated recruitment of the RSC nucleosome-remodeling complex."
      Ng H.H., Robert F., Young R.A., Struhl K.
      Genes Dev. 16:806-819(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae cause dependence on the Hir/Hpc pathway: polymerase passage may degrade chromatin structure."
      Formosa T., Ruone S., Adams M.D., Olsen A.E., Eriksson P., Yu Y., Rhoades A.R., Kaufman P.D., Stillman D.J.
      Genetics 162:1557-1571(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    13. "Replication-independent histone deposition by the HIR complex and Asf1."
      Green E.M., Antczak A.J., Bailey A.O., Franco A.A., Wu K.J., Yates J.R. III, Kaufman P.D.
      Curr. Biol. 15:2044-2049(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH HIR1; HIR3 AND HPC2, INTERACTION WITH ASF1.
    14. "The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF."
      Prochasson P., Florens L., Swanson S.K., Washburn M.P., Workman J.L.
      Genes Dev. 19:2534-2539(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH HIR1; HIR3 AND HPC2.
    15. "Evidence that Spt2/Sin1, an HMG-like factor, plays roles in transcription elongation, chromatin structure, and genome stability in Saccharomyces cerevisiae."
      Nourani A., Robert F., Winston F.
      Mol. Cell. Biol. 26:1496-1509(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    16. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-713, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiHIR2_YEAST
    AccessioniPrimary (citable) accession number: P32480
    Secondary accession number(s): D6W2A5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 135 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 922 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3