Protein HIR1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P32479 (HIR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 136. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein HIR1

Alternative name(s):
Histone transcription regulator 1

Gene names

Name:	HIR1
Ordered Locus Names:	YBL008W
ORF Names:	YBL0318

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	840 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the HIR complex, which cooperates with ASF1 to promote replication-independent chromatin assembly. The HIR complex is also required for the periodic repression of three of the four histone gene loci during cell cycle as well as for autogenous regulation of the HTA1-HTB1 locus by H2A and H2B. DNA-binding by the HIR complex may repress transcription by inhibiting nucleosome remodeling by the SWI/SNF complex. The HIR complex may also be required for transcriptional silencing of centromeric, telomeric and mating-type loci in the absence of CAF-1. Ref.1 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.23
Subunit structure	Component of the HIR complex, composed of HIR1, HIR2, HIR3 and HPC2. This complex may consist of one copy of HIR1 and HIR3 and two copies of HIR2 and HPC2. The HIR complex interacts with ASF1. Interacts with SNF2, SNF5 and SWI3. May interact with SPT4. May self-associate. Ref.5 Ref.6 Ref.9 Ref.10 Ref.11 Ref.19 Ref.20
Subcellular location	Nucleus. Chromosome › centromere. Note: Enriched at centromeres. Ref.12
Miscellaneous	Present with 846 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the WD repeat HIR1 family. Contains 10 WD repeats.
Sequence caution	The sequence L03838 differs from that shown. Reason: Frameshift at positions 33, 243 and 254.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Centromere Chromosome Nucleus
Domain	Repeat WD repeat
Molecular function	Chromatin regulator Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication-independent nucleosome assembly Inferred from direct assay Ref.20 Ref.19. Source: SGD chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.5. Source: SGD transcription elongation from RNA polymerase II promoter Inferred from genetic interaction Ref.23. Source: SGD
Cellular_component	HIR complex Inferred from direct assay Ref.20 Ref.19. Source: SGD chromosome, centromeric region Inferred from direct assay Ref.12. Source: SGD nucleus Inferred from physical interaction Ref.6. Source: SGD
Molecular_function	chromatin binding Inferred from electronic annotation. Source: InterPro transcription corepressor activity Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
itself		4	EBI-8316,EBI-8316
ASF1	P32447	6	EBI-8316,EBI-3003
HIR2	P32480	5	EBI-8316,EBI-8323
SPT4	P32914	2	EBI-8316,EBI-17928

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 840

840

Protein HIR1

PRO_0000051016

Regions

Repeat

15 – 54

WD 1

Repeat

77 – 116

WD 2

Repeat

135 – 174

WD 3

Repeat

177 – 216

WD 4

Repeat

230 – 273

WD 5

Repeat

312 – 350

WD 6

Repeat

354 – 393

WD 7

Repeat

691 – 732

WD 8

Repeat

741 – 780

WD 9

Repeat

799 – 839

WD 10

Region

1 – 389

389

Mediates transcriptional repression

Region

304 – 840

537

Interaction with ASF1

Region

392 – 840

449

Mediates transcriptional repression, self-association and interaction with HIR2

Amino acid modifications

Modified residue

610

Phosphoserine Ref.24 Ref.25 Ref.26

Experimental info

Sequence conflict

L → F Ref.1

Sequence conflict

260

V → M in AAB23989. Ref.2

Sequence conflict

260

V → M in CAA84827. Ref.3

Sequence conflict

340

W → M Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P32479 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 40A2932F3306358A
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FASTA

840

93,858

        10         20         30         40         50         60 
MKVVKFPWLA HREESRKYEI YTVDVSHDGK RLATGGLDGK IRIWSIDSIL RCMELESLTP 

        70         80         90        100        110        120 
EIPLPQDLQM PLCSMSRHTG SITCVKFSPD GKYLASGSDD RILLIWALDE EQSSQPAFGS 

       130        140        150        160        170        180 
EHEREHWTVR KRLVAHDNDI QDICWAPDSS ILVTVGLDRS VIVWNGSTFE KLKRFDVHQS 

       190        200        210        220        230        240 
LVKGVVFDPA NKYFATTSDD RTMKIFRYHK TGDISFTIEH IITEPFKESP LTTYFRRPSW 

       250        260        270        280        290        300 
SPDGQHIAVP NATNGPVSSV AIVNRGTWDT NVSLIGHDAP TEVARFNPRL FERNAGVKQK 

       310        320        330        340        350        360 
KDDDPENALV GQNDDKVHHF DKNIDSVVAT AGQDKSLAVW STSRPRPILV AFDIANKSIT 

       370        380        390        400        410        420 
DMSWNPDGSL LFVASLDSSI TLFKFENNEL GKPIPLEKNM EQLYRYGVDK DSLDFPESIN 

       430        440        450        460        470        480 
QLLLEDQTKS FKHTKISTSK LGENHPTLAT NSASNQKDNN DASVSRSEHI NILIPKRKKD 

       490        500        510        520        530        540 
AILNKAVTLK SGKKRVAPTL ISTSSSSPFS NGIKKPTLDS KRIENNVKSS TKTINSKNTL 

       550        560        570        580        590        600 
LNVPEGVEKK ISISSFPLPR LGIHSLIMGT KERSAWKISN SELENDDADN AGGKGSDGTS 

       610        620        630        640        650        660 
NSIDDIAVLS EEENDFHRMT LNAKLTQEKI WSEEPTTRCL LQSDVIPDTD VVVLEGGSLD 

       670        680        690        700        710        720 
DIAVLEIRNG VERSIQFDSE ALLDNPTRIL GYQGGKRTIE TFIPEVIICA IGSKDCKCWC 

       730        740        750        760        770        780 
LASANGSIYI LSYNGQQRIP KICLGHKVIK MVTSSKYLLV LTERGLFFAW DLLDLKLVLR 

       790        800        810        820        830        840 
NVPILPILNG QPIHGNKVRI NKVIKCFRLD GSSCDLLLEV GDPKNVYKWT KDLGCWSLYK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of HIR1 and HIR2, two genes required for regulation of histone gene transcription in Saccharomyces cerevisiae." Sherwood P.W., Tsang S.V., Osley M.A. Mol. Cell. Biol. 13:28-38(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"Sequence of a 12.7 kb segment of yeast chromosome II identifies a PDR-like gene and several new open reading frames." Delaveau T., Jacq C., Perea J. Yeast 8:761-768(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 260. Strain: ATCC 204508 / S288c.
[5]	"Hir1p and Hir2p function as transcriptional corepressors to regulate histone gene transcription in the Saccharomyces cerevisiae cell cycle." Spector M.S., Raff A., DeSilva H., Lee K., Osley M.A. Mol. Cell. Biol. 17:545-552(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH HIR2.
[6]	"Functional dissection of yeast Hir1p, a WD repeat-containing transcriptional corepressor." DeSilva H., Lee K., Osley M.A. Genetics 148:657-667(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SELF-ASSOCIATION, INTERACTION WITH HIR2 AND SPT4.
[7]	"Yeast Ty1 retrotransposition is stimulated by a synergistic interaction between mutations in chromatin assembly factor I and histone regulatory proteins." Qian Z., Huang H., Hong J.Y., Burck C.L., Johnston S.D., Berman J., Carol A., Liebman S.W. Mol. Cell. Biol. 18:4783-4792(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Hir proteins are required for position-dependent gene silencing in Saccharomyces cerevisiae in the absence of chromatin assembly factor I." Kaufman P.D., Cohen J.L., Osley M.A. Mol. Cell. Biol. 18:4793-4806(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"A role for transcriptional repressors in targeting the yeast Swi/Snf complex." Dimova D., Nackerdien Z., Furgeson S., Eguchi S., Osley M.A. Mol. Cell 4:75-83(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SNF2; SNF5 AND SWI3.
[10]	"Yeast histone deposition protein Asf1p requires Hir proteins and PCNA for heterochromatic silencing." Sharp J.A., Fouts E.T., Krawitz D.C., Kaufman P.D. Curr. Biol. 11:463-473(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH ASF1.
[11]	"Yeast ASF1 protein is required for cell cycle regulation of histone gene transcription." Sutton A., Bucaria J., Osley M.A., Sternglanz R. Genetics 158:587-596(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH ASF1.
[12]	"Chromatin assembly factor I and Hir proteins contribute to building functional kinetochores in S. cerevisiae." Sharp J.A., Franco A.A., Osley M.A., Kaufman P.D. Genes Dev. 16:85-100(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[13]	"Genome-wide location and regulated recruitment of the RSC nucleosome-remodeling complex." Ng H.H., Robert F., Young R.A., Struhl K. Genes Dev. 16:806-819(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[14]	"Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae cause dependence on the Hir/Hpc pathway: polymerase passage may degrade chromatin structure." Formosa T., Ruone S., Adams M.D., Olsen A.E., Eriksson P., Yu Y., Rhoades A.R., Kaufman P.D., Stillman D.J. Genetics 162:1557-1571(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[15]	"Chromatin assembly factor I mutants defective for PCNA binding require Asf1/Hir proteins for silencing." Krawitz D.C., Kama T., Kaufman P.D. Mol. Cell. Biol. 22:614-625(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[16]	"The budding yeast silencing protein Sir1 is a functional component of centromeric chromatin." Sharp J.A., Krawitz D.C., Gardner K.A., Fox C.A., Kaufman P.D. Genes Dev. 17:2356-2361(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[17]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[18]	"Functional roles for evolutionarily conserved Spt4p at centromeres and heterochromatin in Saccharomyces cerevisiae." Crotti L.B., Basrai M.A. EMBO J. 23:1804-1814(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[19]	"Replication-independent histone deposition by the HIR complex and Asf1." Green E.M., Antczak A.J., Bailey A.O., Franco A.A., Wu K.J., Yates J.R. III, Kaufman P.D. Curr. Biol. 15:2044-2049(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH HIR2; HIR3 AND HPC2, INTERACTION WITH ASF1.
[20]	"The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF." Prochasson P., Florens L., Swanson S.K., Washburn M.P., Workman J.L. Genes Dev. 19:2534-2539(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH HIR2; HIR3 AND HPC2.
[21]	"Regulation of histone deposition proteins Asf1/Hir1 by multiple DNA damage checkpoint kinases in Saccharomyces cerevisiae." Sharp J.A., Rizki G., Kaufman P.D. Genetics 171:885-899(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[22]	"Histones are incorporated in trans during reassembly of the yeast PHO5 promoter." Schermer U.J., Korber P., Hoerz W. Mol. Cell 19:279-285(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[23]	"Evidence that Spt2/Sin1, an HMG-like factor, plays roles in transcription elongation, chromatin structure, and genome stability in Saccharomyces cerevisiae." Nourani A., Robert F., Winston F. Mol. Cell. Biol. 26:1496-1509(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[24]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-610, MASS SPECTROMETRY. Strain: ADR376.
[25]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-610, MASS SPECTROMETRY.
[26]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-610, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L03838 Genomic DNA. No translation available. S47695 Genomic DNA. Translation: AAB23989.1. Z35769 Genomic DNA. Translation: CAA84827.1. BK006936 Genomic DNA. Translation: DAA07112.2.
PIR	S41218.
RefSeq	NP_009545.2. NM_001178248.2.
3D structure databases
ProteinModelPortal	P32479.
SMR	P32479. Positions 20-383, 705-732.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2365N.
IntAct	P32479. 32 interactions.
MINT	MINT-707704.
STRING	4932.YBL008W.
Proteomic databases
PaxDb	P32479.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBL008W; YBL008W; YBL008W.
GeneID	852275.
KEGG	sce:YBL008W.
Organism-specific databases
CYGD	YBL008w.
SGD	S000000104. HIR1.
Phylogenomic databases
eggNOG	COG2319.
GeneTree	ENSGT00550000074919.
HOGENOM	HOG000208205.
KO	K11293.
OMA	WAPDSSI.
OrthoDB	EOG4QRMC9.
Gene expression databases
Genevestigator	P32479.
GermOnline	YBL008W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.130.10.10. 2 hits.
InterPro	IPR011494. Hira. IPR019015. HIRA_B_motif. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF07569. Hira. 1 hit. PF09453. HIRA_B. 1 hit. PF00400. WD40. 5 hits. [Graphical view]
SMART	SM00320. WD40. 6 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 2 hits.
PROSITE	PS00678. WD_REPEATS_1. 1 hit. PS50082. WD_REPEATS_2. 4 hits. PS50294. WD_REPEATS_REGION. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	970890.

Entry information

Entry name

HIR1_YEAST

Accession

Primary (citable) accession number: P32479
Secondary accession number(s): D6VPZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 136 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents