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P32479 (HIR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 146. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein HIR1
Alternative name(s):
Histone transcription regulator 1
Gene names
Name:HIR1
Ordered Locus Names:YBL008W
ORF Names:YBL0318
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length840 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the HIR complex, which cooperates with ASF1 to promote replication-independent chromatin assembly. The HIR complex is also required for the periodic repression of three of the four histone gene loci during cell cycle as well as for autogenous regulation of the HTA1-HTB1 locus by H2A and H2B. DNA-binding by the HIR complex may repress transcription by inhibiting nucleosome remodeling by the SWI/SNF complex. The HIR complex may also be required for transcriptional silencing of centromeric, telomeric and mating-type loci in the absence of CAF-1. Ref.1 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.23

Subunit structure

Component of the HIR complex, composed of HIR1, HIR2, HIR3 and HPC2. This complex may consist of one copy of HIR1 and HIR3 and two copies of HIR2 and HPC2. The HIR complex interacts with ASF1. Interacts with SNF2, SNF5 and SWI3. May interact with SPT4. May self-associate. Ref.5 Ref.6 Ref.9 Ref.10 Ref.11 Ref.19 Ref.20

Subcellular location

Nucleus. Chromosomecentromere. Note: Enriched at centromeres. Ref.12

Miscellaneous

Present with 846 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the WD repeat HIR1 family.

Contains 7 WD repeats.

Sequence caution

The sequence L03838 differs from that shown. Reason: Frameshift at positions 33, 243 and 254.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 840840Protein HIR1
PRO_0000051016

Regions

Repeat15 – 5440WD 1
Repeat77 – 11640WD 2
Repeat135 – 17440WD 3
Repeat177 – 21640WD 4
Repeat230 – 27344WD 5
Repeat312 – 35039WD 6
Repeat354 – 39340WD 7
Region1 – 389389Mediates transcriptional repression
Region304 – 840537Interaction with ASF1
Region392 – 840449Mediates transcriptional repression, self-association and interaction with HIR2

Amino acid modifications

Modified residue6101Phosphoserine Ref.24 Ref.25

Experimental info

Sequence conflict321L → F Ref.1
Sequence conflict2601V → M in AAB23989. Ref.2
Sequence conflict2601V → M in CAA84827. Ref.3
Sequence conflict3401W → M Ref.1

Sequences

Sequence LengthMass (Da)Tools
P32479 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 40A2932F3306358A

FASTA84093,858
        10         20         30         40         50         60 
MKVVKFPWLA HREESRKYEI YTVDVSHDGK RLATGGLDGK IRIWSIDSIL RCMELESLTP 

        70         80         90        100        110        120 
EIPLPQDLQM PLCSMSRHTG SITCVKFSPD GKYLASGSDD RILLIWALDE EQSSQPAFGS 

       130        140        150        160        170        180 
EHEREHWTVR KRLVAHDNDI QDICWAPDSS ILVTVGLDRS VIVWNGSTFE KLKRFDVHQS 

       190        200        210        220        230        240 
LVKGVVFDPA NKYFATTSDD RTMKIFRYHK TGDISFTIEH IITEPFKESP LTTYFRRPSW 

       250        260        270        280        290        300 
SPDGQHIAVP NATNGPVSSV AIVNRGTWDT NVSLIGHDAP TEVARFNPRL FERNAGVKQK 

       310        320        330        340        350        360 
KDDDPENALV GQNDDKVHHF DKNIDSVVAT AGQDKSLAVW STSRPRPILV AFDIANKSIT 

       370        380        390        400        410        420 
DMSWNPDGSL LFVASLDSSI TLFKFENNEL GKPIPLEKNM EQLYRYGVDK DSLDFPESIN 

       430        440        450        460        470        480 
QLLLEDQTKS FKHTKISTSK LGENHPTLAT NSASNQKDNN DASVSRSEHI NILIPKRKKD 

       490        500        510        520        530        540 
AILNKAVTLK SGKKRVAPTL ISTSSSSPFS NGIKKPTLDS KRIENNVKSS TKTINSKNTL 

       550        560        570        580        590        600 
LNVPEGVEKK ISISSFPLPR LGIHSLIMGT KERSAWKISN SELENDDADN AGGKGSDGTS 

       610        620        630        640        650        660 
NSIDDIAVLS EEENDFHRMT LNAKLTQEKI WSEEPTTRCL LQSDVIPDTD VVVLEGGSLD 

       670        680        690        700        710        720 
DIAVLEIRNG VERSIQFDSE ALLDNPTRIL GYQGGKRTIE TFIPEVIICA IGSKDCKCWC 

       730        740        750        760        770        780 
LASANGSIYI LSYNGQQRIP KICLGHKVIK MVTSSKYLLV LTERGLFFAW DLLDLKLVLR 

       790        800        810        820        830        840 
NVPILPILNG QPIHGNKVRI NKVIKCFRLD GSSCDLLLEV GDPKNVYKWT KDLGCWSLYK 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of HIR1 and HIR2, two genes required for regulation of histone gene transcription in Saccharomyces cerevisiae."
Sherwood P.W., Tsang S.V., Osley M.A.
Mol. Cell. Biol. 13:28-38(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]"Sequence of a 12.7 kb segment of yeast chromosome II identifies a PDR-like gene and several new open reading frames."
Delaveau T., Jacq C., Perea J.
Yeast 8:761-768(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 260.
Strain: ATCC 204508 / S288c.
[5]"Hir1p and Hir2p function as transcriptional corepressors to regulate histone gene transcription in the Saccharomyces cerevisiae cell cycle."
Spector M.S., Raff A., DeSilva H., Lee K., Osley M.A.
Mol. Cell. Biol. 17:545-552(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH HIR2.
[6]"Functional dissection of yeast Hir1p, a WD repeat-containing transcriptional corepressor."
DeSilva H., Lee K., Osley M.A.
Genetics 148:657-667(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SELF-ASSOCIATION, INTERACTION WITH HIR2 AND SPT4.
[7]"Yeast Ty1 retrotransposition is stimulated by a synergistic interaction between mutations in chromatin assembly factor I and histone regulatory proteins."
Qian Z., Huang H., Hong J.Y., Burck C.L., Johnston S.D., Berman J., Carol A., Liebman S.W.
Mol. Cell. Biol. 18:4783-4792(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Hir proteins are required for position-dependent gene silencing in Saccharomyces cerevisiae in the absence of chromatin assembly factor I."
Kaufman P.D., Cohen J.L., Osley M.A.
Mol. Cell. Biol. 18:4793-4806(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"A role for transcriptional repressors in targeting the yeast Swi/Snf complex."
Dimova D., Nackerdien Z., Furgeson S., Eguchi S., Osley M.A.
Mol. Cell 4:75-83(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SNF2; SNF5 AND SWI3.
[10]"Yeast histone deposition protein Asf1p requires Hir proteins and PCNA for heterochromatic silencing."
Sharp J.A., Fouts E.T., Krawitz D.C., Kaufman P.D.
Curr. Biol. 11:463-473(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ASF1.
[11]"Yeast ASF1 protein is required for cell cycle regulation of histone gene transcription."
Sutton A., Bucaria J., Osley M.A., Sternglanz R.
Genetics 158:587-596(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ASF1.
[12]"Chromatin assembly factor I and Hir proteins contribute to building functional kinetochores in S. cerevisiae."
Sharp J.A., Franco A.A., Osley M.A., Kaufman P.D.
Genes Dev. 16:85-100(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[13]"Genome-wide location and regulated recruitment of the RSC nucleosome-remodeling complex."
Ng H.H., Robert F., Young R.A., Struhl K.
Genes Dev. 16:806-819(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[14]"Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae cause dependence on the Hir/Hpc pathway: polymerase passage may degrade chromatin structure."
Formosa T., Ruone S., Adams M.D., Olsen A.E., Eriksson P., Yu Y., Rhoades A.R., Kaufman P.D., Stillman D.J.
Genetics 162:1557-1571(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[15]"Chromatin assembly factor I mutants defective for PCNA binding require Asf1/Hir proteins for silencing."
Krawitz D.C., Kama T., Kaufman P.D.
Mol. Cell. Biol. 22:614-625(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[16]"The budding yeast silencing protein Sir1 is a functional component of centromeric chromatin."
Sharp J.A., Krawitz D.C., Gardner K.A., Fox C.A., Kaufman P.D.
Genes Dev. 17:2356-2361(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[17]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[18]"Functional roles for evolutionarily conserved Spt4p at centromeres and heterochromatin in Saccharomyces cerevisiae."
Crotti L.B., Basrai M.A.
EMBO J. 23:1804-1814(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[19]"Replication-independent histone deposition by the HIR complex and Asf1."
Green E.M., Antczak A.J., Bailey A.O., Franco A.A., Wu K.J., Yates J.R. III, Kaufman P.D.
Curr. Biol. 15:2044-2049(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH HIR2; HIR3 AND HPC2, INTERACTION WITH ASF1.
[20]"The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF."
Prochasson P., Florens L., Swanson S.K., Washburn M.P., Workman J.L.
Genes Dev. 19:2534-2539(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH HIR2; HIR3 AND HPC2.
[21]"Regulation of histone deposition proteins Asf1/Hir1 by multiple DNA damage checkpoint kinases in Saccharomyces cerevisiae."
Sharp J.A., Rizki G., Kaufman P.D.
Genetics 171:885-899(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[22]"Histones are incorporated in trans during reassembly of the yeast PHO5 promoter."
Schermer U.J., Korber P., Hoerz W.
Mol. Cell 19:279-285(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[23]"Evidence that Spt2/Sin1, an HMG-like factor, plays roles in transcription elongation, chromatin structure, and genome stability in Saccharomyces cerevisiae."
Nourani A., Robert F., Winston F.
Mol. Cell. Biol. 26:1496-1509(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[24]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-610, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[25]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-610, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[26]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L03838 Genomic DNA. No translation available.
S47695 Genomic DNA. Translation: AAB23989.1.
Z35769 Genomic DNA. Translation: CAA84827.1.
BK006936 Genomic DNA. Translation: DAA07112.2.
PIRS41218.
RefSeqNP_009545.2. NM_001178248.2.

3D structure databases

ProteinModelPortalP32479.
SMRP32479. Positions 20-383.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32691. 202 interactions.
DIPDIP-2365N.
IntActP32479. 31 interactions.
MINTMINT-707704.
STRING4932.YBL008W.

Proteomic databases

MaxQBP32479.
PaxDbP32479.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBL008W; YBL008W; YBL008W.
GeneID852275.
KEGGsce:YBL008W.

Organism-specific databases

CYGDYBL008w.
SGDS000000104. HIR1.

Phylogenomic databases

eggNOGCOG2319.
GeneTreeENSGT00550000074919.
HOGENOMHOG000208205.
KOK11293.
OMADICWAPD.
OrthoDBEOG7NCVC9.

Enzyme and pathway databases

BioCycYEAST:G3O-28914-MONOMER.

Gene expression databases

GenevestigatorP32479.

Family and domain databases

Gene3D2.130.10.10. 2 hits.
InterProIPR011494. Hira.
IPR019015. HIRA_B_motif.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF07569. Hira. 1 hit.
PF09453. HIRA_B. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
SMARTSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 4 hits.
PROSITEPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio970890.
PROP32479.

Entry information

Entry nameHIR1_YEAST
AccessionPrimary (citable) accession number: P32479
Secondary accession number(s): D6VPZ2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: July 27, 2011
Last modified: June 11, 2014
This is version 146 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families