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P32479

- HIR1_YEAST

UniProt

P32479 - HIR1_YEAST

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Protein

Protein HIR1

Gene

HIR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the HIR complex, which cooperates with ASF1 to promote replication-independent chromatin assembly. The HIR complex is also required for the periodic repression of three of the four histone gene loci during cell cycle as well as for autogenous regulation of the HTA1-HTB1 locus by H2A and H2B. DNA-binding by the HIR complex may repress transcription by inhibiting nucleosome remodeling by the SWI/SNF complex. The HIR complex may also be required for transcriptional silencing of centromeric, telomeric and mating-type loci in the absence of CAF-1.19 Publications

GO - Molecular functioni

  1. chromatin binding Source: InterPro
  2. identical protein binding Source: IntAct
  3. transcription corepressor activity Source: SGD

GO - Biological processi

  1. chromatin modification Source: UniProtKB-KW
  2. DNA replication-independent nucleosome assembly Source: SGD
  3. regulation of transcription from RNA polymerase II promoter Source: SGD
  4. transcription elongation from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-28914-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein HIR1
Alternative name(s):
Histone transcription regulator 1
Gene namesi
Name:HIR1
Ordered Locus Names:YBL008W
ORF Names:YBL0318
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBL008w.
SGDiS000000104. HIR1.

Subcellular locationi

Nucleus 1 Publication. Chromosomecentromere 1 Publication
Note: Enriched at centromeres.

GO - Cellular componenti

  1. chromosome, centromeric region Source: SGD
  2. HIR complex Source: SGD
  3. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 840840Protein HIR1PRO_0000051016Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei610 – 6101Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32479.
PaxDbiP32479.

Expressioni

Gene expression databases

GenevestigatoriP32479.

Interactioni

Subunit structurei

Component of the HIR complex, composed of HIR1, HIR2, HIR3 and HPC2. This complex may consist of one copy of HIR1 and HIR3 and two copies of HIR2 and HPC2. The HIR complex interacts with ASF1. Interacts with SNF2, SNF5 and SWI3. May interact with SPT4. May self-associate.7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-8316,EBI-8316
ASF1P324476EBI-8316,EBI-3003
HIR2P324805EBI-8316,EBI-8323
SPT4P329142EBI-8316,EBI-17928

Protein-protein interaction databases

BioGridi32691. 203 interactions.
DIPiDIP-2365N.
IntActiP32479. 31 interactions.
MINTiMINT-707704.
STRINGi4932.YBL008W.

Structurei

3D structure databases

ProteinModelPortaliP32479.
SMRiP32479. Positions 20-383.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati15 – 5440WD 1Add
BLAST
Repeati77 – 11640WD 2Add
BLAST
Repeati135 – 17440WD 3Add
BLAST
Repeati177 – 21640WD 4Add
BLAST
Repeati230 – 27344WD 5Add
BLAST
Repeati312 – 35039WD 6Add
BLAST
Repeati354 – 39340WD 7Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 389389Mediates transcriptional repressionAdd
BLAST
Regioni304 – 840537Interaction with ASF1Add
BLAST
Regioni392 – 840449Mediates transcriptional repression, self-association and interaction with HIR2Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat HIR1 family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00550000074919.
HOGENOMiHOG000208205.
InParanoidiP32479.
KOiK11293.
OMAiDICWAPD.
OrthoDBiEOG7NCVC9.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR011494. Hira.
IPR019015. HIRA_B_motif.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF07569. Hira. 1 hit.
PF09453. HIRA_B. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 4 hits.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32479-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVVKFPWLA HREESRKYEI YTVDVSHDGK RLATGGLDGK IRIWSIDSIL
60 70 80 90 100
RCMELESLTP EIPLPQDLQM PLCSMSRHTG SITCVKFSPD GKYLASGSDD
110 120 130 140 150
RILLIWALDE EQSSQPAFGS EHEREHWTVR KRLVAHDNDI QDICWAPDSS
160 170 180 190 200
ILVTVGLDRS VIVWNGSTFE KLKRFDVHQS LVKGVVFDPA NKYFATTSDD
210 220 230 240 250
RTMKIFRYHK TGDISFTIEH IITEPFKESP LTTYFRRPSW SPDGQHIAVP
260 270 280 290 300
NATNGPVSSV AIVNRGTWDT NVSLIGHDAP TEVARFNPRL FERNAGVKQK
310 320 330 340 350
KDDDPENALV GQNDDKVHHF DKNIDSVVAT AGQDKSLAVW STSRPRPILV
360 370 380 390 400
AFDIANKSIT DMSWNPDGSL LFVASLDSSI TLFKFENNEL GKPIPLEKNM
410 420 430 440 450
EQLYRYGVDK DSLDFPESIN QLLLEDQTKS FKHTKISTSK LGENHPTLAT
460 470 480 490 500
NSASNQKDNN DASVSRSEHI NILIPKRKKD AILNKAVTLK SGKKRVAPTL
510 520 530 540 550
ISTSSSSPFS NGIKKPTLDS KRIENNVKSS TKTINSKNTL LNVPEGVEKK
560 570 580 590 600
ISISSFPLPR LGIHSLIMGT KERSAWKISN SELENDDADN AGGKGSDGTS
610 620 630 640 650
NSIDDIAVLS EEENDFHRMT LNAKLTQEKI WSEEPTTRCL LQSDVIPDTD
660 670 680 690 700
VVVLEGGSLD DIAVLEIRNG VERSIQFDSE ALLDNPTRIL GYQGGKRTIE
710 720 730 740 750
TFIPEVIICA IGSKDCKCWC LASANGSIYI LSYNGQQRIP KICLGHKVIK
760 770 780 790 800
MVTSSKYLLV LTERGLFFAW DLLDLKLVLR NVPILPILNG QPIHGNKVRI
810 820 830 840
NKVIKCFRLD GSSCDLLLEV GDPKNVYKWT KDLGCWSLYK
Length:840
Mass (Da):93,858
Last modified:July 27, 2011 - v3
Checksum:i40A2932F3306358A
GO

Sequence cautioni

The sequence L03838 differs from that shown. Reason: Frameshift at positions 33, 243 and 254. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti32 – 321L → F(PubMed:8417331)Curated
Sequence conflicti260 – 2601V → M in AAB23989. (PubMed:1441753)Curated
Sequence conflicti260 – 2601V → M in CAA84827. (PubMed:7813418)Curated
Sequence conflicti340 – 3401W → M(PubMed:8417331)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03838 Genomic DNA. No translation available.
S47695 Genomic DNA. Translation: AAB23989.1.
Z35769 Genomic DNA. Translation: CAA84827.1.
BK006936 Genomic DNA. Translation: DAA07112.2.
PIRiS41218.
RefSeqiNP_009545.2. NM_001178248.2.

Genome annotation databases

EnsemblFungiiYBL008W; YBL008W; YBL008W.
GeneIDi852275.
KEGGisce:YBL008W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03838 Genomic DNA. No translation available.
S47695 Genomic DNA. Translation: AAB23989.1 .
Z35769 Genomic DNA. Translation: CAA84827.1 .
BK006936 Genomic DNA. Translation: DAA07112.2 .
PIRi S41218.
RefSeqi NP_009545.2. NM_001178248.2.

3D structure databases

ProteinModelPortali P32479.
SMRi P32479. Positions 20-383.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32691. 203 interactions.
DIPi DIP-2365N.
IntActi P32479. 31 interactions.
MINTi MINT-707704.
STRINGi 4932.YBL008W.

Proteomic databases

MaxQBi P32479.
PaxDbi P32479.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBL008W ; YBL008W ; YBL008W .
GeneIDi 852275.
KEGGi sce:YBL008W.

Organism-specific databases

CYGDi YBL008w.
SGDi S000000104. HIR1.

Phylogenomic databases

eggNOGi COG2319.
GeneTreei ENSGT00550000074919.
HOGENOMi HOG000208205.
InParanoidi P32479.
KOi K11293.
OMAi DICWAPD.
OrthoDBi EOG7NCVC9.

Enzyme and pathway databases

BioCyci YEAST:G3O-28914-MONOMER.

Miscellaneous databases

NextBioi 970890.
PROi P32479.

Gene expression databases

Genevestigatori P32479.

Family and domain databases

Gene3Di 2.130.10.10. 2 hits.
InterProi IPR011494. Hira.
IPR019015. HIRA_B_motif.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF07569. Hira. 1 hit.
PF09453. HIRA_B. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view ]
SMARTi SM00320. WD40. 6 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 4 hits.
PROSITEi PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of HIR1 and HIR2, two genes required for regulation of histone gene transcription in Saccharomyces cerevisiae."
    Sherwood P.W., Tsang S.V., Osley M.A.
    Mol. Cell. Biol. 13:28-38(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. "Sequence of a 12.7 kb segment of yeast chromosome II identifies a PDR-like gene and several new open reading frames."
    Delaveau T., Jacq C., Perea J.
    Yeast 8:761-768(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 260.
    Strain: ATCC 204508 / S288c.
  5. "Hir1p and Hir2p function as transcriptional corepressors to regulate histone gene transcription in the Saccharomyces cerevisiae cell cycle."
    Spector M.S., Raff A., DeSilva H., Lee K., Osley M.A.
    Mol. Cell. Biol. 17:545-552(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HIR2.
  6. "Functional dissection of yeast Hir1p, a WD repeat-containing transcriptional corepressor."
    DeSilva H., Lee K., Osley M.A.
    Genetics 148:657-667(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SELF-ASSOCIATION, INTERACTION WITH HIR2 AND SPT4.
  7. "Yeast Ty1 retrotransposition is stimulated by a synergistic interaction between mutations in chromatin assembly factor I and histone regulatory proteins."
    Qian Z., Huang H., Hong J.Y., Burck C.L., Johnston S.D., Berman J., Carol A., Liebman S.W.
    Mol. Cell. Biol. 18:4783-4792(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Hir proteins are required for position-dependent gene silencing in Saccharomyces cerevisiae in the absence of chromatin assembly factor I."
    Kaufman P.D., Cohen J.L., Osley M.A.
    Mol. Cell. Biol. 18:4793-4806(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "A role for transcriptional repressors in targeting the yeast Swi/Snf complex."
    Dimova D., Nackerdien Z., Furgeson S., Eguchi S., Osley M.A.
    Mol. Cell 4:75-83(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SNF2; SNF5 AND SWI3.
  10. "Yeast histone deposition protein Asf1p requires Hir proteins and PCNA for heterochromatic silencing."
    Sharp J.A., Fouts E.T., Krawitz D.C., Kaufman P.D.
    Curr. Biol. 11:463-473(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ASF1.
  11. "Yeast ASF1 protein is required for cell cycle regulation of histone gene transcription."
    Sutton A., Bucaria J., Osley M.A., Sternglanz R.
    Genetics 158:587-596(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ASF1.
  12. "Chromatin assembly factor I and Hir proteins contribute to building functional kinetochores in S. cerevisiae."
    Sharp J.A., Franco A.A., Osley M.A., Kaufman P.D.
    Genes Dev. 16:85-100(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  13. "Genome-wide location and regulated recruitment of the RSC nucleosome-remodeling complex."
    Ng H.H., Robert F., Young R.A., Struhl K.
    Genes Dev. 16:806-819(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. "Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae cause dependence on the Hir/Hpc pathway: polymerase passage may degrade chromatin structure."
    Formosa T., Ruone S., Adams M.D., Olsen A.E., Eriksson P., Yu Y., Rhoades A.R., Kaufman P.D., Stillman D.J.
    Genetics 162:1557-1571(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  15. "Chromatin assembly factor I mutants defective for PCNA binding require Asf1/Hir proteins for silencing."
    Krawitz D.C., Kama T., Kaufman P.D.
    Mol. Cell. Biol. 22:614-625(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  16. "The budding yeast silencing protein Sir1 is a functional component of centromeric chromatin."
    Sharp J.A., Krawitz D.C., Gardner K.A., Fox C.A., Kaufman P.D.
    Genes Dev. 17:2356-2361(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  17. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  18. "Functional roles for evolutionarily conserved Spt4p at centromeres and heterochromatin in Saccharomyces cerevisiae."
    Crotti L.B., Basrai M.A.
    EMBO J. 23:1804-1814(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  19. "Replication-independent histone deposition by the HIR complex and Asf1."
    Green E.M., Antczak A.J., Bailey A.O., Franco A.A., Wu K.J., Yates J.R. III, Kaufman P.D.
    Curr. Biol. 15:2044-2049(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH HIR2; HIR3 AND HPC2, INTERACTION WITH ASF1.
  20. "The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF."
    Prochasson P., Florens L., Swanson S.K., Washburn M.P., Workman J.L.
    Genes Dev. 19:2534-2539(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH HIR2; HIR3 AND HPC2.
  21. "Regulation of histone deposition proteins Asf1/Hir1 by multiple DNA damage checkpoint kinases in Saccharomyces cerevisiae."
    Sharp J.A., Rizki G., Kaufman P.D.
    Genetics 171:885-899(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  22. "Histones are incorporated in trans during reassembly of the yeast PHO5 promoter."
    Schermer U.J., Korber P., Hoerz W.
    Mol. Cell 19:279-285(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  23. "Evidence that Spt2/Sin1, an HMG-like factor, plays roles in transcription elongation, chromatin structure, and genome stability in Saccharomyces cerevisiae."
    Nourani A., Robert F., Winston F.
    Mol. Cell. Biol. 26:1496-1509(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  24. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-610, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  25. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-610, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  26. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHIR1_YEAST
AccessioniPrimary (citable) accession number: P32479
Secondary accession number(s): D6VPZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 149 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 846 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3