Reviewed, UniProtKB/Swiss-Prot P32478 (HS150_YEAST)

Names and origin

Protein names

Recommended name:
    Cell wall mannoprotein HSP150
Alternative name(s):
    150 kDa heat shock glycoprotein
    Protein with internal repeats 2
    Covalently-linked cell wall protein 7

Gene names

Name:	HSP150
Synonyms:	CCW7, ORE1, PIR2
Ordered Locus Names:	YJL159W
ORF Names:	J0558

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the outer cell wall layer. Required for stability of the cell wall and for optimal growth. Required for resistance against several antifungal and cell wall-perturbing agents and for tolerance to heat shock. Ref.12 Ref.13 Ref.15 Ref.18
Subcellular location	Secreted › cell wall. Note: Covalently attached to the cell wall. Ref.12 Ref.13 Ref.5 Ref.20
Induction	Positively regulated by signaling through MPK1 in response to cell wall perturbation. Induced by heat shock and nitrogen starvation. Expression is also regulated by the ACE2 and SWI5 transcription factors. Ref.12 Ref.14 Ref.16 Ref.19 Ref.22
Domain	The PIR1/2/3 repeats are required for the covalent linkage to the cell wall By similarity. Their number varies among different strains of S.cerevisiae.
Post-translational modification	Covalently linked to beta-1,3-glucan of the inner cell wall layer via an alkali-sensitive ester linkage between the gamma-carboxyl group of glutamic acids, arising from specific glutamines within the PIR1/2/3 repeats, and hydroxyl groups of glucoses of beta-1,3-glucan chains By similarity. The propeptide is cleaved off in the late Golgi. While both peptides are secreted, only a fraction of the mature glycoprotein is incorporated into the cell wall. Ref.5 O-glycosylated. Extensively O-mannosylated. Ref.5
Miscellaneous	Present with 14600 molecules/cell in log phase SD medium. Ref.17
Sequence similarities	Belongs to the PIR protein family. Contains 11 PIR1/2/3 repeats.
Sequence caution	The sequence CAA89454.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Stress response
Cellular component	Cell wall Secreted
Domain	Repeat Signal
PTM	Cleavage on pair of basic residues Glycoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall organization Ref.15 Inferred from mutant phenotype. Source: SGD response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from direct assay. Source: SGD fungal-type cell wall Ref.12 Ref.13 Ref.20 Inferred from direct assay. Source: SGD
Molecular function	structural constituent of cell wall Ref.13 Inferred from physical interaction. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Ref.5 Ref.1
	Propeptide	19 – 72	54			PRO_0000033260
	Chain	73 – 413	341	Cell wall mannoprotein HSP150		PRO_0000033261
Regions
	Repeat	73 – 89	17	PIR1/2/3 1
	Repeat	97 – 115	19	PIR1/2/3 2
	Repeat	116 – 134	19	PIR1/2/3 3
	Repeat	140 – 158	19	PIR1/2/3 4
	Repeat	164 – 182	19	PIR1/2/3 5
	Repeat	188 – 206	19	PIR1/2/3 6
	Repeat	207 – 225	19	PIR1/2/3 7
	Repeat	226 – 244	19	PIR1/2/3 8
	Repeat	245 – 263	19	PIR1/2/3 9
	Repeat	264 – 282	19	PIR1/2/3 10
	Repeat	283 – 300	18	PIR1/2/3 11
Sites
	Site	72 – 73	2	Cleavage; by KEX2
	Site	81	1	Covalent attachment to cell wall glycan By similarity
	Site	107	1	Covalent attachment to cell wall glycan By similarity
	Site	126	1	Covalent attachment to cell wall glycan By similarity
	Site	150	1	Covalent attachment to cell wall glycan By similarity
	Site	174	1	Covalent attachment to cell wall glycan By similarity
	Site	198	1	Covalent attachment to cell wall glycan By similarity
	Site	217	1	Covalent attachment to cell wall glycan By similarity
	Site	236	1	Covalent attachment to cell wall glycan By similarity
	Site	255	1	Covalent attachment to cell wall glycan By similarity
	Site	274	1	Covalent attachment to cell wall glycan By similarity
	Site	292	1	Covalent attachment to cell wall glycan By similarity
Natural variations
	Natural variant	96	1	T → S in strain: B1, TD04/1a and TD04/1b. Ref.6
	Natural variant	116 – 163	48	Missing in strain: B1, TD04/1a and TD04/1b.
	Natural variant	175	1	V → I in strain: TD04/1a and TD04/1b. Ref.6
	Natural variant	176	1	Q → QAATTTASVSTKSSAAAVSQ IGDGQIQATTKTTAAAVSQI GDGQIQ in strain: TD04/1b.
	Natural variant	199	1	V → I in strain: B1, TD04/1a and TD04/1b. Ref.6
	Natural variant	226 – 244	19	Missing in strain: B1, TD04/1a and TD04/1b.
	Natural variant	297	1	T → S in strain: B1, TD04/1a and TD04/1b. Ref.6
Experimental info
	Sequence conflict	47	1	G → A in BAA02886. Ref.3
	Sequence conflict	121 – 123	3	QIG → R in CAA89454. Ref.4
	Sequence conflict	152 – 175	24	Missing in CAA89454. Ref.4
	Sequence conflict	211	1	S → F in AAA17683. Ref.1
	Sequence conflict	211	1	S → F in AAB23364. Ref.2
	Sequence conflict	219	1	Q → L in AAA17683. Ref.1
	Sequence conflict	219	1	Q → L in AAB23364. Ref.2
	Sequence conflict	226	1	A → R in AAA17683. Ref.1
	Sequence conflict	226	1	A → R in AAB23364. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P32478-1 [UniParc]. Last modified August 30, 2005. Version 2. Checksum: 5EAF10136E25132A	FASTA	413	41,059
	10 20 30 40 50 60 MQYKKTLVAS ALAATTLAAY APSEPWSTLT PTATYSGGVT DYASTFGIAV QPISTTSSAS 70 80 90 100 110 120 SAATTASSKA KRAASQIGDG QVQAATTTAS VSTKSTAAAV SQIGDGQIQA TTKTTAAAVS 130 140 150 160 170 180 QIGDGQIQAT TKTTSAKTTA AAVSQISDGQ IQATTTTLAP KSTAAAVSQI GDGQVQATTT 190 200 210 220 230 240 TLAPKSTAAA VSQIGDGQVQ ATTKTTAAAV SQIGDGQVQA TTKTTAAAVS QIGDGQVQAT 250 260 270 280 290 300 TKTTAAAVSQ IGDGQVQATT KTTAAAVSQI TDGQVQATTK TTQAASQVSD GQVQATTATS 310 320 330 340 350 360 ASAAATSTDP VDAVSCKTSG TLEMNLKGGI LTDGKGRIGS IVANRQFQFD GPPPQAGAIY 370 380 390 400 410 AAGWSITPDG NLAIGDNDVF YQCLSGTFYN LYDEHIGSQC TPVHLEAIDL IDC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A heat shock gene from Saccharomyces cerevisiae encoding a secretory glycoprotein." Russo P., Kalkkinen N., Sareneva H., Paakkola J., Makarow M. Proc. Natl. Acad. Sci. U.S.A. 89:3671-3675(1992) [PubMed: 1570286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, PROTEIN SEQUENCE OF 19-39; 73-94; 114-132; 243-261; 346-370 AND 390-412.
[2]	Erratum Russo P., Kalkkinen N., Sareneva H., Paakkola J., Makarow M. Proc. Natl. Acad. Sci. U.S.A. 89:8857-8857(1992) [PubMed: 1528903] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"Three yeast genes, PIR1, PIR2 and PIR3, containing internal tandem repeats, are related to each other, and PIR1 and PIR2 are required for tolerance to heat shock." Toh-e A., Yasunaga S., Nisogi H., Tanaka K., Oguchi T., Matsui Y. Yeast 9:481-494(1993) [PubMed: 8322511] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RAY-3AD.
[4]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	"Specific labelling of cell wall proteins by biotinylation. Identification of four covalently linked O-mannosylated proteins of Saccharomyces cerevisiae." Mrsa V., Seidl T., Gentzsch M., Tanner W. Yeast 13:1145-1154(1997) [PubMed: 9301021] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-29 AND 73-87, CLEAVAGE BY KEX2, GLYCOSYLATION, SUBCELLULAR LOCATION.
[6]	"Characterization of Ccw7p cell wall proteins and the encoding genes of Saccharomyces cerevisiae wine yeast strains: relevance for flor formation." Kovacs M., Stuparevic I., Mrsa V., Maraz A. FEMS Yeast Res. 8:1115-1126(2008) [PubMed: 18657192] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 33-393, PROTEIN SEQUENCE OF 73-84, VARIANTS SER-96; 116-ALA--THR-163 DEL ILE-175; ILE-199; 226-ALA--THR-244 DEL AND SER-297. Strain: B1, TD04/1a and TD04/1b.
[7]	"Two-dimensional analysis of proteins secreted by Saccharomyces cerevisiae regenerating protoplasts: a novel approach to study the cell wall." Pardo M., Monteoliva L., Pla J., Sanchez M., Gil C., Nombela C. Yeast 15:459-472(1999) [PubMed: 10234784] [Abstract] Cited for: PROTEIN SEQUENCE OF 73-82.
[8]	"Minisatellites in Saccharomyces cerevisiae genes encoding cell wall proteins: a new way towards wine strain characterisation." Marinangeli P., Angelozzi D., Ciani M., Clementi F., Mannazzu I. FEMS Yeast Res. 4:427-435(2004) [PubMed: 14734023] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 100-366. Strain: ATCC 204508 / S288c.
[9]	"YJL159w does encode Pir2/Hsp150." Moukadiri I., Zueco J. Yeast 18:323-324(2001) [PubMed: 11223941] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 267-413. Strain: ATCC 204508 / S288c.
[10]	"Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii." Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P. Genome Biol. 4:R45.1-R45.13(2003) [PubMed: 12844361] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 289-406. Strain: ATCC 204511 / S288c / AB972.
[11]	"A proteomic approach for the study of Saccharomyces cerevisiae cell wall biogenesis." Pardo M., Ward M., Bains S., Molina M., Blackstock W., Gil C., Nombela C. Electrophoresis 21:3396-3410(2000) [PubMed: 11079560] [Abstract] Cited for: PROTEIN SEQUENCE OF 318-327 AND 338-345.
[12]	"Stress proteins on the yeast cell surface determine resistance to osmotin, a plant antifungal protein." Yun D.-J., Zhao Y., Pardo J.M., Narasimhan M.L., Damsz B., Lee H., Abad L.R., D'Urzo M.P., Hasegawa P.M., Bressan R.A. Proc. Natl. Acad. Sci. U.S.A. 94:7082-7087(1997) [PubMed: 9192695] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
[13]	"The contribution of the O-glycosylated protein Pir2p/Hsp150 to the construction of the yeast cell wall in wild-type cells and beta 1,6-glucan-deficient mutants." Kapteyn J.C., Van Egmond P., Sievi E., Van Den Ende H., Makarow M., Klis F.M. Mol. Microbiol. 31:1835-1844(1999) [PubMed: 10209754] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[14]	"Genome-wide analysis of gene expression regulated by the yeast cell wall integrity signalling pathway." Jung U.S., Levin D.E. Mol. Microbiol. 34:1049-1057(1999) [PubMed: 10594829] [Abstract] Cited for: INDUCTION.
[15]	"Role of NaOH-extractable cell wall proteins Ccw5p, Ccw6p, Ccw7p and Ccw8p (members of the Pir protein family) in stability of the Saccharomyces cerevisiae cell wall." Mrsa V., Tanner W. Yeast 15:813-820(1999) [PubMed: 10407261] [Abstract] Cited for: FUNCTION.
[16]	"Overlapping and distinct roles of the duplicated yeast transcription factors Ace2p and Swi5p." Doolin M.-T., Johnson A.L., Johnston L.H., Butler G. Mol. Microbiol. 40:422-432(2001) [PubMed: 11309124] [Abstract] Cited for: INDUCTION.
[17]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[18]	"Increased mortality of Saccharomyces cerevisiae cell wall protein mutants." Teparic R., Stuparevic I., Mrsa V. Microbiology 150:3145-3150(2004) [PubMed: 15470095] [Abstract] Cited for: FUNCTION.
[19]	"Characterization of the transcriptional response to cell wall stress in Saccharomyces cerevisiae." Boorsma A., de Nobel H., ter Riet B., Bargmann B., Brul S., Hellingwerf K.J., Klis F.M. Yeast 21:413-427(2004) [PubMed: 15116342] [Abstract] Cited for: INDUCTION.
[20]	"Comprehensive proteomic analysis of Saccharomyces cerevisiae cell walls: identification of proteins covalently attached via glycosylphosphatidylinositol remnants or mild alkali-sensitive linkages." Yin Q.Y., de Groot P.W.J., Dekker H.L., de Jong L., Klis F.M., de Koster C.G. J. Biol. Chem. 280:20894-20901(2005) [PubMed: 15781460] [Abstract] Cited for: SUBCELLULAR LOCATION, MASS SPECTROMETRY.
[21]	"Intragenic tandem repeats generate functional variability." Verstrepen K.J., Jansen A., Lewitter F., Fink G.R. Nat. Genet. 37:986-990(2005) [PubMed: 16086015] [Abstract] Cited for: REPEATS.
[22]	"Mass spectrometric quantitation of covalently bound cell wall proteins in Saccharomyces cerevisiae." Yin Q.Y., de Groot P.W.J., de Jong L., Klis F.M., de Koster C.G. FEMS Yeast Res. 7:887-896(2007) [PubMed: 17617218] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M88698 Unassigned DNA. Translation: AAA17683.1. S45000 Genomic DNA. Translation: AAB23364.1. D13741 Genomic DNA. Translation: BAA02886.1. Z49434 Genomic DNA. Translation: CAA89454.1. Sequence problems. EU220722 Genomic DNA. Translation: ABY67720.1. EU220723 Genomic DNA. Translation: ABY67721.1. EU220724 Genomic DNA. Translation: ABY67722.1. AY321583 Genomic DNA. Translation: AAQ83898.1. AY260881 Genomic DNA. Translation: AAP21749.1.
PIR	A46183. S56942.
RefSeq	NP_012376.3.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2035N.
IntAct	P32478. 6 interactions.
STRING	P32478.
2-D gel databases
COMPLUYEAST-2DPAGE	P32478.
Proteomic databases
PeptideAtlas	P32478.
Genome annotation databases
Ensembl	YJL159W; YJL159W; YJL159W; Saccharomyces cerevisiae. [Genome view]
GeneID	853281.
KEGG	sce:YJL159W.
Organism-specific databases
CYGD	YJL159w.
SGD	S000003695. HSP150.
Phylogenomic databases
OrthoDB	EOG9JDJQC.
Gene expression databases
ArrayExpress	P32478.
Genevestigator	P32478.
GermOnline	YJL159W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000420. Yeast_PIR. [Graphical view]
Pfam	PF00399. PIR. 11 hits. [Graphical view]
PROSITE	PS00929. PIR_REPEAT_1. 11 hits. PS50256. PIR_REPEAT_2. 11 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	973573.

Entry information

Entry name

HS150_YEAST

Accession

Primary (citable) accession number: P32478
Secondary accession number(s): B6RI02 , B6RI03, B6RI04, P47000, Q03179, Q6VXX4, Q86ZT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	August 30, 2005
Last modified:	December 15, 2009
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)