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P32477 (GSH1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--cysteine ligase
EC=6.3.2.2
Alternative name(s):
Gamma-ECS
Short name=GCS
Gamma-glutamylcysteine synthetase
Gene names
Name:GSH1
Ordered Locus Names:YJL101C
ORF Names:J0832
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length678 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.

Enzyme regulation

Feedback inhibition by glutathione.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.

Miscellaneous

Present with 5130 molecules/cell in log phase SD medium. Ref.6

Sequence similarities

Belongs to the glutamate--cysteine ligase type 3 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 678678Glutamate--cysteine ligase
PRO_0000192572

Amino acid modifications

Modified residue1161Phosphoserine Ref.7

Experimental info

Sequence conflict2991A → R in CAA78960. Ref.2
Sequence conflict4921L → V in CAA78960. Ref.2
Sequence conflict526 – 5272IL → MV in CAA78960. Ref.2
Sequence conflict5421D → V in CAA78960. Ref.2
Sequence conflict5501I → M in CAA78960. Ref.2

Secondary structure

1 ........................................................................................................ 678
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P32477 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 65A345988A0C5AAC

FASTA67878,254
        10         20         30         40         50         60 
MGLLALGTPL QWFESRTYNE HIRDEGIEQL LYIFQAAGKR DNDPLFWGDE LEYMVVDFDD 

        70         80         90        100        110        120 
KERNSMLDVC HDKILTELNM EDSSLCEAND VSFHPEYGRY MLEATPASPY LNYVGSYVEV 

       130        140        150        160        170        180 
NMQKRRAIAE YKLSEYARQD SKNNLHVGSR SVPLTLTVFP RMGCPDFINI KDPWNHKNAA 

       190        200        210        220        230        240 
SRSLFLPDEV INRHVRFPNL TASIRTRRGE KVCMNVPMYK DIATPETDDS IYDRDWFLPE 

       250        260        270        280        290        300 
DKEAKLASKP GFIYMDSMGF GMGCSCLQVT FQAPNINKAR YLYDALVNFA PIMLAFSAAA 

       310        320        330        340        350        360 
PAFKGWLADQ DVRWNVISGA VDDRTPKERG VAPLLPKYNK NGFGGIAKDV QDKVLEIPKS 

       370        380        390        400        410        420 
RYSSVDLFLG GSKFFNRTYN DTNVPINEKV LGRLLENDKA PLDYDLAKHF AHLYIRDPVS 

       430        440        450        460        470        480 
TFEELLNQDN KTSSNHFENI QSTNWQTLRF KPPTQQATPD KKDSPGWRVE FRPFEVQLLD 

       490        500        510        520        530        540 
FENAAYSVLI YLIVDSILTF SDNINAYIHM SKVWENMKIA HHRDAILFEK FHWKKSFRND 

       550        560        570        580        590        600 
TDVETEDYSI SEIFHNPENG IFPQFVTPIL CQKGFVTKDW KELKHSSKHE RLYYYLKLIS 

       610        620        630        640        650        660 
DRASGELPTT AKFFRNFVLQ HPDYKHDSKI SKSINYDLLS TCDRLTHLDD SKGELTSFLG 

       670 
AEIAEYVKKN KPSIESKC

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of the gamma-glutamylcysteine synthetase gene of Saccharomyces cerevisiae."
Ohtake Y., Yabuuchi S.
Yeast 7:953-961(1991) [PubMed: 1687097] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A high copy number of yeast gamma-glutamylcysteine synthetase suppresses a nuclear mutation affecting mitochondrial translation."
Lisowsky T.
Curr. Genet. 23:408-413(1993) [PubMed: 8100487] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"A 37.5 kb region of yeast chromosome X includes the SME1, MEF2, GSH1 and CSD3 genes, a TCP-1-related gene, an open reading frame similar to the DAL80 gene, and a tRNA(Arg)."
Rasmussen S.W.
Yeast 11:873-883(1995) [PubMed: 7483851] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[5]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-116, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D90220 Genomic DNA. Translation: BAA14251.1.
Z17312 Genomic DNA. Translation: CAA78960.1.
X85021 Genomic DNA. Translation: CAA59393.1.
Z49376 Genomic DNA. Translation: CAA89396.1.
BK006943 Genomic DNA. Translation: DAA08699.1.
PIRS28648.
RefSeqNP_012434.1. NM_001181534.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3IG5X-ray2.10A1-678[»]
3IG8X-ray2.69A1-678[»]
3LVVX-ray2.20A1-678[»]
3LVWX-ray2.50A1-678[»]
ProteinModelPortalP32477.
SMRP32477. Positions 2-673.
ModBaseSearch...

Protein-protein interaction databases

IntActP32477. 10 interactions.
STRINGP32477.

Proteomic databases

PeptideAtlasP32477.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL101C; YJL101C; YJL101C.
GeneID853344.
KEGGsce:YJL101C.
NMPDRfig|4932.3.peg.3403.

Organism-specific databases

CYGDYJL101c.
SGDS000003637. GSH1.

Phylogenomic databases

eggNOGfuNOG05294.
GeneTreeEFGT00050000004278.
HOGENOMHBG331200.
OMAWNVISGA.
OrthoDBEOG4S1XGD.

Enzyme and pathway databases

BRENDA6.3.2.2. 984.

Gene expression databases

ArrayExpressP32477.
GenevestigatorP32477.
GermOnlineYJL101C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR004308. GCS.
[Graphical view]
KOK11204.
PANTHERPTHR11164. GCS. 1 hit.
PfamPF03074. GCS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973735.

Entry information

Entry nameGSH1_YEAST
AccessionPrimary (citable) accession number: P32477
Secondary accession number(s): D6VW83
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: January 25, 2012
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents