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Protein

Glutamate--cysteine ligase

Gene

GSH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.

Enzyme regulationi

Feedback inhibition by glutathione.

Pathwayi: glutathione biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase (GSH1)
  2. Glutathione synthetase (GSH2)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glutamate-cysteine ligase activity Source: SGD

GO - Biological processi

  • glutathione biosynthetic process Source: SGD
  • response to cadmium ion Source: SGD
  • response to hydrogen peroxide Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YJL101C-MONOMER.
BRENDAi6.3.2.2. 984.
UniPathwayiUPA00142; UER00209.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--cysteine ligase (EC:6.3.2.2)
Alternative name(s):
Gamma-ECS
Short name:
GCS
Gamma-glutamylcysteine synthetase
Gene namesi
Name:GSH1
Ordered Locus Names:YJL101C
ORF Names:J0832
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL101C.
SGDiS000003637. GSH1.

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 678678Glutamate--cysteine ligasePRO_0000192572Add
BLAST

Proteomic databases

MaxQBiP32477.
PeptideAtlasiP32477.

Interactioni

Protein-protein interaction databases

BioGridi33656. 169 interactions.
IntActiP32477. 10 interactions.
MINTiMINT-4490897.

Structurei

Secondary structure

1
678
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 154Combined sources
Helixi16 – 183Combined sources
Helixi19 – 3719Combined sources
Beta strandi46 – 5914Combined sources
Turni60 – 634Combined sources
Beta strandi64 – 674Combined sources
Helixi74 – 774Combined sources
Turni78 – 825Combined sources
Helixi83 – 886Combined sources
Beta strandi91 – 944Combined sources
Beta strandi101 – 1088Combined sources
Beta strandi110 – 1123Combined sources
Helixi117 – 14226Combined sources
Beta strandi149 – 1546Combined sources
Turni160 – 1634Combined sources
Beta strandi168 – 1714Combined sources
Turni179 – 1813Combined sources
Helixi188 – 1903Combined sources
Helixi196 – 20813Combined sources
Beta strandi214 – 2185Combined sources
Helixi240 – 2423Combined sources
Helixi244 – 2474Combined sources
Beta strandi252 – 2554Combined sources
Helixi259 – 2624Combined sources
Beta strandi266 – 27510Combined sources
Helixi276 – 28611Combined sources
Helixi289 – 2968Combined sources
Beta strandi306 – 3105Combined sources
Helixi314 – 3207Combined sources
Turni326 – 3305Combined sources
Turni336 – 3383Combined sources
Helixi340 – 3423Combined sources
Helixi348 – 3536Combined sources
Beta strandi361 – 3644Combined sources
Helixi377 – 3793Combined sources
Helixi388 – 3958Combined sources
Helixi404 – 41310Combined sources
Helixi423 – 4253Combined sources
Turni430 – 4323Combined sources
Helixi435 – 4417Combined sources
Beta strandi447 – 4515Combined sources
Beta strandi467 – 4715Combined sources
Helixi480 – 49920Combined sources
Turni500 – 5034Combined sources
Helixi510 – 51910Combined sources
Helixi525 – 5284Combined sources
Beta strandi530 – 5356Combined sources
Beta strandi537 – 5393Combined sources
Beta strandi545 – 5495Combined sources
Helixi550 – 5556Combined sources
Turni557 – 5593Combined sources
Helixi561 – 5644Combined sources
Helixi566 – 5727Combined sources
Helixi580 – 5856Combined sources
Turni587 – 5893Combined sources
Helixi590 – 60314Combined sources
Helixi610 – 61910Combined sources
Beta strandi626 – 6294Combined sources
Helixi632 – 64615Combined sources
Helixi650 – 6523Combined sources
Helixi654 – 66916Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3IG5X-ray2.10A1-678[»]
3IG8X-ray2.69A1-678[»]
3LVVX-ray2.20A1-678[»]
3LVWX-ray2.50A1-678[»]
ProteinModelPortaliP32477.
SMRiP32477. Positions 2-673.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32477.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000011908.
HOGENOMiHOG000199354.
InParanoidiP32477.
KOiK11204.
OMAiPIYRGYL.
OrthoDBiEOG773XQR.

Family and domain databases

InterProiIPR004308. GCS.
[Graphical view]
PANTHERiPTHR11164. PTHR11164. 2 hits.
PfamiPF03074. GCS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32477-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLLALGTPL QWFESRTYNE HIRDEGIEQL LYIFQAAGKR DNDPLFWGDE
60 70 80 90 100
LEYMVVDFDD KERNSMLDVC HDKILTELNM EDSSLCEAND VSFHPEYGRY
110 120 130 140 150
MLEATPASPY LNYVGSYVEV NMQKRRAIAE YKLSEYARQD SKNNLHVGSR
160 170 180 190 200
SVPLTLTVFP RMGCPDFINI KDPWNHKNAA SRSLFLPDEV INRHVRFPNL
210 220 230 240 250
TASIRTRRGE KVCMNVPMYK DIATPETDDS IYDRDWFLPE DKEAKLASKP
260 270 280 290 300
GFIYMDSMGF GMGCSCLQVT FQAPNINKAR YLYDALVNFA PIMLAFSAAA
310 320 330 340 350
PAFKGWLADQ DVRWNVISGA VDDRTPKERG VAPLLPKYNK NGFGGIAKDV
360 370 380 390 400
QDKVLEIPKS RYSSVDLFLG GSKFFNRTYN DTNVPINEKV LGRLLENDKA
410 420 430 440 450
PLDYDLAKHF AHLYIRDPVS TFEELLNQDN KTSSNHFENI QSTNWQTLRF
460 470 480 490 500
KPPTQQATPD KKDSPGWRVE FRPFEVQLLD FENAAYSVLI YLIVDSILTF
510 520 530 540 550
SDNINAYIHM SKVWENMKIA HHRDAILFEK FHWKKSFRND TDVETEDYSI
560 570 580 590 600
SEIFHNPENG IFPQFVTPIL CQKGFVTKDW KELKHSSKHE RLYYYLKLIS
610 620 630 640 650
DRASGELPTT AKFFRNFVLQ HPDYKHDSKI SKSINYDLLS TCDRLTHLDD
660 670
SKGELTSFLG AEIAEYVKKN KPSIESKC
Length:678
Mass (Da):78,254
Last modified:October 1, 1993 - v1
Checksum:i65A345988A0C5AAC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti299 – 2991A → R in CAA78960 (PubMed:8100487).Curated
Sequence conflicti492 – 4921L → V in CAA78960 (PubMed:8100487).Curated
Sequence conflicti526 – 5272IL → MV in CAA78960 (PubMed:8100487).Curated
Sequence conflicti542 – 5421D → V in CAA78960 (PubMed:8100487).Curated
Sequence conflicti550 – 5501I → M in CAA78960 (PubMed:8100487).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90220 Genomic DNA. Translation: BAA14251.1.
Z17312 Genomic DNA. Translation: CAA78960.1.
X85021 Genomic DNA. Translation: CAA59393.1.
Z49376 Genomic DNA. Translation: CAA89396.1.
BK006943 Genomic DNA. Translation: DAA08699.1.
PIRiS28648.
RefSeqiNP_012434.1. NM_001181534.1.

Genome annotation databases

EnsemblFungiiYJL101C; YJL101C; YJL101C.
GeneIDi853344.
KEGGisce:YJL101C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90220 Genomic DNA. Translation: BAA14251.1.
Z17312 Genomic DNA. Translation: CAA78960.1.
X85021 Genomic DNA. Translation: CAA59393.1.
Z49376 Genomic DNA. Translation: CAA89396.1.
BK006943 Genomic DNA. Translation: DAA08699.1.
PIRiS28648.
RefSeqiNP_012434.1. NM_001181534.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3IG5X-ray2.10A1-678[»]
3IG8X-ray2.69A1-678[»]
3LVVX-ray2.20A1-678[»]
3LVWX-ray2.50A1-678[»]
ProteinModelPortaliP32477.
SMRiP32477. Positions 2-673.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33656. 169 interactions.
IntActiP32477. 10 interactions.
MINTiMINT-4490897.

Proteomic databases

MaxQBiP32477.
PeptideAtlasiP32477.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL101C; YJL101C; YJL101C.
GeneIDi853344.
KEGGisce:YJL101C.

Organism-specific databases

EuPathDBiFungiDB:YJL101C.
SGDiS000003637. GSH1.

Phylogenomic databases

GeneTreeiENSGT00390000011908.
HOGENOMiHOG000199354.
InParanoidiP32477.
KOiK11204.
OMAiPIYRGYL.
OrthoDBiEOG773XQR.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00209.
BioCyciYEAST:YJL101C-MONOMER.
BRENDAi6.3.2.2. 984.

Miscellaneous databases

EvolutionaryTraceiP32477.
PROiP32477.

Family and domain databases

InterProiIPR004308. GCS.
[Graphical view]
PANTHERiPTHR11164. PTHR11164. 2 hits.
PfamiPF03074. GCS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the gamma-glutamylcysteine synthetase gene of Saccharomyces cerevisiae."
    Ohtake Y., Yabuuchi S.
    Yeast 7:953-961(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "A high copy number of yeast gamma-glutamylcysteine synthetase suppresses a nuclear mutation affecting mitochondrial translation."
    Lisowsky T.
    Curr. Genet. 23:408-413(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "A 37.5 kb region of yeast chromosome X includes the SME1, MEF2, GSH1 and CSD3 genes, a TCP-1-related gene, an open reading frame similar to the DAL80 gene, and a tRNA(Arg)."
    Rasmussen S.W.
    Yeast 11:873-883(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  4. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGSH1_YEAST
AccessioniPrimary (citable) accession number: P32477
Secondary accession number(s): D6VW83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 8, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5130 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.