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Protein

Glutamate--cysteine ligase

Gene

GSH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.

Enzyme regulationi

Feedback inhibition by glutathione.

Pathwayi: glutathione biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase (GSH1)
  2. Glutathione synthetase (GSH2)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glutamate-cysteine ligase activity Source: SGD

GO - Biological processi

  • glutathione biosynthetic process Source: SGD
  • response to cadmium ion Source: SGD
  • response to hydrogen peroxide Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YJL101C-MONOMER.
BRENDAi6.3.2.2. 984.
UniPathwayiUPA00142; UER00209.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--cysteine ligase (EC:6.3.2.2)
Alternative name(s):
Gamma-ECS
Short name:
GCS
Gamma-glutamylcysteine synthetase
Gene namesi
Name:GSH1
Ordered Locus Names:YJL101C
ORF Names:J0832
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL101C.
SGDiS000003637. GSH1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • intracellular Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001925721 – 678Glutamate--cysteine ligaseAdd BLAST678

Proteomic databases

MaxQBiP32477.
PRIDEiP32477.

Interactioni

Protein-protein interaction databases

BioGridi33656. 169 interactors.
IntActiP32477. 10 interactors.
MINTiMINT-4490897.

Structurei

Secondary structure

1678
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 15Combined sources4
Helixi16 – 18Combined sources3
Helixi19 – 37Combined sources19
Beta strandi46 – 59Combined sources14
Turni60 – 63Combined sources4
Beta strandi64 – 67Combined sources4
Helixi74 – 77Combined sources4
Turni78 – 82Combined sources5
Helixi83 – 88Combined sources6
Beta strandi91 – 94Combined sources4
Beta strandi101 – 108Combined sources8
Beta strandi110 – 112Combined sources3
Helixi117 – 142Combined sources26
Beta strandi149 – 154Combined sources6
Turni160 – 163Combined sources4
Beta strandi168 – 171Combined sources4
Turni179 – 181Combined sources3
Helixi188 – 190Combined sources3
Helixi196 – 208Combined sources13
Beta strandi214 – 218Combined sources5
Helixi240 – 242Combined sources3
Helixi244 – 247Combined sources4
Beta strandi252 – 255Combined sources4
Helixi259 – 262Combined sources4
Beta strandi266 – 275Combined sources10
Helixi276 – 286Combined sources11
Helixi289 – 296Combined sources8
Beta strandi306 – 310Combined sources5
Helixi314 – 320Combined sources7
Turni326 – 330Combined sources5
Turni336 – 338Combined sources3
Helixi340 – 342Combined sources3
Helixi348 – 353Combined sources6
Beta strandi361 – 364Combined sources4
Helixi377 – 379Combined sources3
Helixi388 – 395Combined sources8
Helixi404 – 413Combined sources10
Helixi423 – 425Combined sources3
Turni430 – 432Combined sources3
Helixi435 – 441Combined sources7
Beta strandi447 – 451Combined sources5
Beta strandi467 – 471Combined sources5
Helixi480 – 499Combined sources20
Turni500 – 503Combined sources4
Helixi510 – 519Combined sources10
Helixi525 – 528Combined sources4
Beta strandi530 – 535Combined sources6
Beta strandi537 – 539Combined sources3
Beta strandi545 – 549Combined sources5
Helixi550 – 555Combined sources6
Turni557 – 559Combined sources3
Helixi561 – 564Combined sources4
Helixi566 – 572Combined sources7
Helixi580 – 585Combined sources6
Turni587 – 589Combined sources3
Helixi590 – 603Combined sources14
Helixi610 – 619Combined sources10
Beta strandi626 – 629Combined sources4
Helixi632 – 646Combined sources15
Helixi650 – 652Combined sources3
Helixi654 – 669Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3IG5X-ray2.10A1-678[»]
3IG8X-ray2.69A1-678[»]
3LVVX-ray2.20A1-678[»]
3LVWX-ray2.50A1-678[»]
ProteinModelPortaliP32477.
SMRiP32477.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32477.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000011908.
HOGENOMiHOG000199354.
InParanoidiP32477.
KOiK11204.
OMAiRFKPPTQ.
OrthoDBiEOG092C1O5L.

Family and domain databases

InterProiIPR004308. GCS.
[Graphical view]
PANTHERiPTHR11164. PTHR11164. 2 hits.
PfamiPF03074. GCS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32477-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLLALGTPL QWFESRTYNE HIRDEGIEQL LYIFQAAGKR DNDPLFWGDE
60 70 80 90 100
LEYMVVDFDD KERNSMLDVC HDKILTELNM EDSSLCEAND VSFHPEYGRY
110 120 130 140 150
MLEATPASPY LNYVGSYVEV NMQKRRAIAE YKLSEYARQD SKNNLHVGSR
160 170 180 190 200
SVPLTLTVFP RMGCPDFINI KDPWNHKNAA SRSLFLPDEV INRHVRFPNL
210 220 230 240 250
TASIRTRRGE KVCMNVPMYK DIATPETDDS IYDRDWFLPE DKEAKLASKP
260 270 280 290 300
GFIYMDSMGF GMGCSCLQVT FQAPNINKAR YLYDALVNFA PIMLAFSAAA
310 320 330 340 350
PAFKGWLADQ DVRWNVISGA VDDRTPKERG VAPLLPKYNK NGFGGIAKDV
360 370 380 390 400
QDKVLEIPKS RYSSVDLFLG GSKFFNRTYN DTNVPINEKV LGRLLENDKA
410 420 430 440 450
PLDYDLAKHF AHLYIRDPVS TFEELLNQDN KTSSNHFENI QSTNWQTLRF
460 470 480 490 500
KPPTQQATPD KKDSPGWRVE FRPFEVQLLD FENAAYSVLI YLIVDSILTF
510 520 530 540 550
SDNINAYIHM SKVWENMKIA HHRDAILFEK FHWKKSFRND TDVETEDYSI
560 570 580 590 600
SEIFHNPENG IFPQFVTPIL CQKGFVTKDW KELKHSSKHE RLYYYLKLIS
610 620 630 640 650
DRASGELPTT AKFFRNFVLQ HPDYKHDSKI SKSINYDLLS TCDRLTHLDD
660 670
SKGELTSFLG AEIAEYVKKN KPSIESKC
Length:678
Mass (Da):78,254
Last modified:October 1, 1993 - v1
Checksum:i65A345988A0C5AAC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti299A → R in CAA78960 (PubMed:8100487).Curated1
Sequence conflicti492L → V in CAA78960 (PubMed:8100487).Curated1
Sequence conflicti526 – 527IL → MV in CAA78960 (PubMed:8100487).Curated2
Sequence conflicti542D → V in CAA78960 (PubMed:8100487).Curated1
Sequence conflicti550I → M in CAA78960 (PubMed:8100487).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90220 Genomic DNA. Translation: BAA14251.1.
Z17312 Genomic DNA. Translation: CAA78960.1.
X85021 Genomic DNA. Translation: CAA59393.1.
Z49376 Genomic DNA. Translation: CAA89396.1.
BK006943 Genomic DNA. Translation: DAA08699.1.
PIRiS28648.
RefSeqiNP_012434.1. NM_001181534.1.

Genome annotation databases

EnsemblFungiiYJL101C; YJL101C; YJL101C.
GeneIDi853344.
KEGGisce:YJL101C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90220 Genomic DNA. Translation: BAA14251.1.
Z17312 Genomic DNA. Translation: CAA78960.1.
X85021 Genomic DNA. Translation: CAA59393.1.
Z49376 Genomic DNA. Translation: CAA89396.1.
BK006943 Genomic DNA. Translation: DAA08699.1.
PIRiS28648.
RefSeqiNP_012434.1. NM_001181534.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3IG5X-ray2.10A1-678[»]
3IG8X-ray2.69A1-678[»]
3LVVX-ray2.20A1-678[»]
3LVWX-ray2.50A1-678[»]
ProteinModelPortaliP32477.
SMRiP32477.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33656. 169 interactors.
IntActiP32477. 10 interactors.
MINTiMINT-4490897.

Proteomic databases

MaxQBiP32477.
PRIDEiP32477.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL101C; YJL101C; YJL101C.
GeneIDi853344.
KEGGisce:YJL101C.

Organism-specific databases

EuPathDBiFungiDB:YJL101C.
SGDiS000003637. GSH1.

Phylogenomic databases

GeneTreeiENSGT00390000011908.
HOGENOMiHOG000199354.
InParanoidiP32477.
KOiK11204.
OMAiRFKPPTQ.
OrthoDBiEOG092C1O5L.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00209.
BioCyciYEAST:YJL101C-MONOMER.
BRENDAi6.3.2.2. 984.

Miscellaneous databases

EvolutionaryTraceiP32477.
PROiP32477.

Family and domain databases

InterProiIPR004308. GCS.
[Graphical view]
PANTHERiPTHR11164. PTHR11164. 2 hits.
PfamiPF03074. GCS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSH1_YEAST
AccessioniPrimary (citable) accession number: P32477
Secondary accession number(s): D6VW83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5130 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.