Reviewed, UniProtKB/Swiss-Prot P32477 (GSH1_YEAST)

Names and origin

Protein names

Recommended name:
    Glutamate--cysteine ligase
    EC=6.3.2.2
Alternative name(s):
    Gamma-glutamylcysteine synthetase
    Gamma-ECS
      Short name=GCS

Gene names

Name:	GSH1
Ordered Locus Names:	YJL101C
ORF Names:	J0832

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	678 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.
Enzyme regulation	Feedback inhibition by glutathione.
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
Miscellaneous	Present with 5130 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the glutamate--cysteine ligase type 3 family.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Ref.1 Inferred from direct assay. Source: SGD response to cadmium ion Inferred from direct assay. Source: SGD response to cold Inferred from expression pattern. Source: SGD response to drug Inferred from mutant phenotype. Source: SGD response to heat Inferred from expression pattern. Source: SGD response to hydrogen peroxide Inferred from mutant phenotype. Source: SGD response to mercury ion Inferred from expression pattern. Source: SGD
Cellular component	intracellular Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-cysteine ligase activity Ref.1 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 678	678	Glutamate--cysteine ligase		PRO_0000192572
Amino acid modifications
	Modified residue	116	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	299	1	A → R in CAA78960. Ref.2
	Sequence conflict	492	1	L → V in CAA78960. Ref.2
	Sequence conflict	526 – 527	2	IL → MV in CAA78960. Ref.2
	Sequence conflict	542	1	D → V in CAA78960. Ref.2
	Sequence conflict	550	1	I → M in CAA78960. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P32477-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 65A345988A0C5AAC	FASTA	678	78,254
	10 20 30 40 50 60 MGLLALGTPL QWFESRTYNE HIRDEGIEQL LYIFQAAGKR DNDPLFWGDE LEYMVVDFDD 70 80 90 100 110 120 KERNSMLDVC HDKILTELNM EDSSLCEAND VSFHPEYGRY MLEATPASPY LNYVGSYVEV 130 140 150 160 170 180 NMQKRRAIAE YKLSEYARQD SKNNLHVGSR SVPLTLTVFP RMGCPDFINI KDPWNHKNAA 190 200 210 220 230 240 SRSLFLPDEV INRHVRFPNL TASIRTRRGE KVCMNVPMYK DIATPETDDS IYDRDWFLPE 250 260 270 280 290 300 DKEAKLASKP GFIYMDSMGF GMGCSCLQVT FQAPNINKAR YLYDALVNFA PIMLAFSAAA 310 320 330 340 350 360 PAFKGWLADQ DVRWNVISGA VDDRTPKERG VAPLLPKYNK NGFGGIAKDV QDKVLEIPKS 370 380 390 400 410 420 RYSSVDLFLG GSKFFNRTYN DTNVPINEKV LGRLLENDKA PLDYDLAKHF AHLYIRDPVS 430 440 450 460 470 480 TFEELLNQDN KTSSNHFENI QSTNWQTLRF KPPTQQATPD KKDSPGWRVE FRPFEVQLLD 490 500 510 520 530 540 FENAAYSVLI YLIVDSILTF SDNINAYIHM SKVWENMKIA HHRDAILFEK FHWKKSFRND 550 560 570 580 590 600 TDVETEDYSI SEIFHNPENG IFPQFVTPIL CQKGFVTKDW KELKHSSKHE RLYYYLKLIS 610 620 630 640 650 660 DRASGELPTT AKFFRNFVLQ HPDYKHDSKI SKSINYDLLS TCDRLTHLDD SKGELTSFLG 670 AEIAEYVKKN KPSIESKC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the gamma-glutamylcysteine synthetase gene of Saccharomyces cerevisiae." Ohtake Y., Yabuuchi S. Yeast 7:953-961(1991) [PubMed: 1687097] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A high copy number of yeast gamma-glutamylcysteine synthetase suppresses a nuclear mutation affecting mitochondrial translation." Lisowsky T. Curr. Genet. 23:408-413(1993) [PubMed: 8100487] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A 37.5 kb region of yeast chromosome X includes the SME1, MEF2, GSH1 and CSD3 genes, a TCP-1-related gene, an open reading frame similar to the DAL80 gene, and a tRNA(Arg)." Rasmussen S.W. Yeast 11:873-883(1995) [PubMed: 7483851] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-116, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D90220 Genomic DNA. Translation: BAA14251.1. Z17312 Genomic DNA. Translation: CAA78960.1. X85021 Genomic DNA. Translation: CAA59393.1. Z49376 Genomic DNA. Translation: CAA89396.1.
PIR	S28648.
RefSeq	NP_012434.1.
3D structure databases
ModBase	Search...
Proteomic databases
PeptideAtlas	P32477.
Genome annotation databases
Ensembl	YJL101C. Saccharomyces cerevisiae. [Contig view]
GeneID	853344.
GenomeReviews	Gene locus YJL101C in contig Y13136_GR.
KEGG	sce:YJL101C.
NMPDR	fig|4932.3.peg.3403.
Organism-specific databases
CYGD	YJL101c.
SGD	S000003637. GSH1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P32477.
OMA	P32477. FNTVEDN.
Enzyme and pathway databases
BRENDA	6.3.2.2. 250.
Gene expression databases
ArrayExpress	P32477.
GermOnline	YJL101C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004308. GCS. [Graphical view]
PANTHER	PTHR11164. GCS. 1 hit.
Pfam	PF03074. GCS. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	973735.

Entry information

Entry name

GSH1_YEAST

Accession

Primary (citable) accession number: P32477

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)