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P32476

- ERG1_YEAST

UniProt

P32476 - ERG1_YEAST

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Protein

Squalene monooxygenase

Gene

ERG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway.

Catalytic activityi

Squalene + NADPH + O2 = (3S)-2,3-epoxy-2,3-dihydrosqualene + NADP+ + H2O.

Cofactori

FAD.

Enzyme regulationi

Inhibited by the allylamine antimycotic drugs.

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi21 – 4828FADSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. drug binding Source: SGD
  2. flavin adenine dinucleotide binding Source: InterPro
  3. squalene monooxygenase activity Source: SGD

GO - Biological processi

  1. ergosterol biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

BioCyciMetaCyc:YGR175C-MONOMER.
YEAST:YGR175C-MONOMER.
ReactomeiREACT_188982. Activation of gene expression by SREBF (SREBP).
UniPathwayiUPA00767; UER00752.

Names & Taxonomyi

Protein namesi
Recommended name:
Squalene monooxygenase (EC:1.14.13.132)
Alternative name(s):
Squalene epoxidase
Short name:
SE
Gene namesi
Name:ERG1
Ordered Locus Names:YGR175C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR175c.
SGDiS000003407. ERG1.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: SGD
  2. integral component of membrane Source: UniProtKB-KW
  3. lipid particle Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 496496Squalene monooxygenasePRO_0000209851Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki284 – 284Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki311 – 311Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP32476.
PaxDbiP32476.
PeptideAtlasiP32476.

Expressioni

Gene expression databases

GenevestigatoriP32476.

Interactioni

Subunit structurei

Interacts with ERG28.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CSG2P352061EBI-6545,EBI-2051140
ERG25P530451EBI-6545,EBI-6506
ERG4P253401EBI-6545,EBI-6558
ERP1Q053591EBI-6545,EBI-6581
ERP4Q124501EBI-6545,EBI-6598
ERV25P548371EBI-6545,EBI-6642
ERV29P533371EBI-6545,EBI-23662
GOT1Q035541EBI-6545,EBI-2057821
PIS1P061971EBI-6545,EBI-13458
SNA3P143591EBI-6545,EBI-26122
SWP1Q027951EBI-6545,EBI-12666
YHR146WP388451EBI-6545,EBI-24770
YVH1Q022561EBI-6545,EBI-14322

Protein-protein interaction databases

BioGridi33428. 41 interactions.
DIPiDIP-6325N.
IntActiP32476. 16 interactions.
MINTiMINT-1324933.
STRINGi4932.YGR175C.

Structurei

3D structure databases

ProteinModelPortaliP32476.
SMRiP32476. Positions 20-50.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini38 – 474437LumenalSequence AnalysisAdd
BLAST
Topological domaini496 – 4961CytoplasmicSequence Analysis

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei17 – 3721HelicalSequence AnalysisAdd
BLAST
Transmembranei475 – 49521HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the squalene monooxygenase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0654.
GeneTreeiENSGT00390000011759.
HOGENOMiHOG000174713.
InParanoidiP32476.
KOiK00511.
OMAiFITLANR.
OrthoDBiEOG7KM62S.

Family and domain databases

InterProiIPR003042. Rng_hydrolase-like.
IPR013698. Squalene_epoxidase.
[Graphical view]
PfamiPF08491. SE. 1 hit.
[Graphical view]
PRINTSiPR00420. RNGMNOXGNASE.

Sequencei

Sequence statusi: Complete.

P32476-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSAVNVAPEL INADNTITYD AIVIGAGVIG PCVATGLARK GKKVLIVERD
60 70 80 90 100
WAMPDRIVGE LMQPGGVRAL RSLGMIQSIN NIEAYPVTGY TVFFNGEQVD
110 120 130 140 150
IPYPYKADIP KVEKLKDLVK DGNDKVLEDS TIHIKDYEDD ERERGVAFVH
160 170 180 190 200
GRFLNNLRNI TAQEPNVTRV QGNCIEILKD EKNEVVGAKV DIDGRGKVEF
210 220 230 240 250
KAHLTFICDG IFSRFRKELH PDHVPTVGSS FVGMSLFNAK NPAPMHGHVI
260 270 280 290 300
LGSDHMPILV YQISPEETRI LCAYNSPKVP ADIKSWMIKD VQPFIPKSLR
310 320 330 340 350
PSFDEAVSQG KFRAMPNSYL PARQNDVTGM CVIGDALNMR HPLTGGGMTV
360 370 380 390 400
GLHDVVLLIK KIGDLDFSDR EKVLDELLDY HFERKSYDSV INVLSVALYS
410 420 430 440 450
LFAADSDNLK ALQKGCFKYF QRGGDCVNKP VEFLSGVLPK PLQLTRVFFA
460 470 480 490
VAFYTIYLNM EERGFLGLPM ALLEGIMILI TAIRVFTPFL FGELIG
Length:496
Mass (Da):55,126
Last modified:October 1, 1996 - v2
Checksum:iBBEFD766634855E8
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti251 – 2511L → F in strain: A2-M8; confers resistance to the allylamine antifungal terbinafine.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64994 Genomic DNA. Translation: AAA34592.1.
Z72960 Genomic DNA. Translation: CAA97201.1.
BK006941 Genomic DNA. Translation: DAA08271.1.
PIRiS64489.
RefSeqiNP_011691.1. NM_001181304.1.

Genome annotation databases

EnsemblFungiiYGR175C; YGR175C; YGR175C.
GeneIDi853086.
KEGGisce:YGR175C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64994 Genomic DNA. Translation: AAA34592.1 .
Z72960 Genomic DNA. Translation: CAA97201.1 .
BK006941 Genomic DNA. Translation: DAA08271.1 .
PIRi S64489.
RefSeqi NP_011691.1. NM_001181304.1.

3D structure databases

ProteinModelPortali P32476.
SMRi P32476. Positions 20-50.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33428. 41 interactions.
DIPi DIP-6325N.
IntActi P32476. 16 interactions.
MINTi MINT-1324933.
STRINGi 4932.YGR175C.

Chemistry

ChEMBLi CHEMBL1888.

Proteomic databases

MaxQBi P32476.
PaxDbi P32476.
PeptideAtlasi P32476.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR175C ; YGR175C ; YGR175C .
GeneIDi 853086.
KEGGi sce:YGR175C.

Organism-specific databases

CYGDi YGR175c.
SGDi S000003407. ERG1.

Phylogenomic databases

eggNOGi COG0654.
GeneTreei ENSGT00390000011759.
HOGENOMi HOG000174713.
InParanoidi P32476.
KOi K00511.
OMAi FITLANR.
OrthoDBi EOG7KM62S.

Enzyme and pathway databases

UniPathwayi UPA00767 ; UER00752 .
BioCyci MetaCyc:YGR175C-MONOMER.
YEAST:YGR175C-MONOMER.
Reactomei REACT_188982. Activation of gene expression by SREBF (SREBP).

Miscellaneous databases

NextBioi 973064.
PROi P32476.

Gene expression databases

Genevestigatori P32476.

Family and domain databases

InterProi IPR003042. Rng_hydrolase-like.
IPR013698. Squalene_epoxidase.
[Graphical view ]
Pfami PF08491. SE. 1 hit.
[Graphical view ]
PRINTSi PR00420. RNGMNOXGNASE.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The gene encoding squalene epoxidase from Saccharomyces cerevisiae: cloning and characterization."
    Jandrositz A., Turnowsky F., Hoegenauer G.
    Gene 107:155-160(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: A2-M8.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-311.
    Strain: SUB592.
  6. "A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
    Hitchcock A.L., Auld K., Gygi S.P., Silver P.A.
    Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-284.
  7. "Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex."
    Mo C., Bard M.
    J. Lipid Res. 46:1991-1998(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ERG28.
  8. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiERG1_YEAST
AccessioniPrimary (citable) accession number: P32476
Secondary accession number(s): D6VUW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 65400 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3