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Protein

Squalene monooxygenase

Gene

ERG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway.1 Publication

Miscellaneous

Present with 65400 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Squalene + [reduced NADPH--hemoprotein reductase] + O2 = (3S)-2,3-epoxy-2,3-dihydrosqualene + [oxidized NADPH--hemoprotein reductase] + H2O.1 Publication

Cofactori

Enzyme regulationi

Inhibited by the allylamine antimycotic drugs.

Kineticsi

  1. KM=13.5 µM for squalene1 Publication

    pH dependencei

    Optimum pH is 7.5.1 Publication

    Pathwayi: lanosterol biosynthesis

    This protein is involved in step 2 of the subpathway that synthesizes lanosterol from farnesyl diphosphate.
    Proteins known to be involved in the 3 steps of the subpathway in this organism are:
    1. Squalene synthase (ERG9)
    2. Squalene monooxygenase (ERG1)
    3. Lanosterol synthase (ERG7)
    This subpathway is part of the pathway lanosterol biosynthesis, which is itself part of Terpene metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes lanosterol from farnesyl diphosphate, the pathway lanosterol biosynthesis and in Terpene metabolism.

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi21 – 48FADSequence analysisAdd BLAST28

    GO - Molecular functioni

    • drug binding Source: SGD
    • flavin adenine dinucleotide binding Source: InterPro
    • squalene monooxygenase activity Source: SGD

    GO - Biological processi

    • ergosterol biosynthetic process Source: SGD

    Keywordsi

    Molecular functionOxidoreductase
    LigandFAD, Flavoprotein, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:YGR175C-MONOMER.
    YEAST:YGR175C-MONOMER.
    ReactomeiR-SCE-191273. Cholesterol biosynthesis.
    UniPathwayiUPA00767; UER00752.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Squalene monooxygenase (EC:1.14.14.171 Publication)
    Alternative name(s):
    Squalene epoxidase1 Publication
    Short name:
    SE
    Gene namesi
    Name:ERG1
    Ordered Locus Names:YGR175C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome VII

    Organism-specific databases

    EuPathDBiFungiDB:YGR175C.
    SGDiS000003407. ERG1.

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 16CytoplasmicSequence analysisAdd BLAST16
    Transmembranei17 – 37HelicalSequence analysisAdd BLAST21
    Topological domaini38 – 474LumenalSequence analysisAdd BLAST437
    Transmembranei475 – 495HelicalSequence analysisAdd BLAST21
    Topological domaini496CytoplasmicSequence analysis1

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    Pathology & Biotechi

    Chemistry databases

    ChEMBLiCHEMBL1888.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002098511 – 496Squalene monooxygenaseAdd BLAST496

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Cross-linki284Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
    Cross-linki289Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
    Cross-linki311Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

    Keywords - PTMi

    Isopeptide bond, Ubl conjugation

    Proteomic databases

    MaxQBiP32476.
    PRIDEiP32476.
    TopDownProteomicsiP32476.

    PTM databases

    iPTMnetiP32476.

    Interactioni

    Subunit structurei

    Interacts with ERG28.1 Publication

    Protein-protein interaction databases

    BioGridi33428. 45 interactors.
    DIPiDIP-6325N.
    IntActiP32476. 17 interactors.
    MINTiMINT-1324933.
    STRINGi4932.YGR175C.

    Structurei

    3D structure databases

    ProteinModelPortaliP32476.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the squalene monooxygenase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    GeneTreeiENSGT00390000011759.
    HOGENOMiHOG000174713.
    InParanoidiP32476.
    KOiK00511.
    OMAiSSHFVGC.
    OrthoDBiEOG092C1N2D.

    Family and domain databases

    Gene3Di3.50.50.60. 1 hit.
    InterProiView protein in InterPro
    IPR036188. FAD/NAD-bd_sf.
    IPR013698. Squalene_epoxidase.
    PfamiView protein in Pfam
    PF08491. SE. 1 hit.
    SUPFAMiSSF51905. SSF51905. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    P32476-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSAVNVAPEL INADNTITYD AIVIGAGVIG PCVATGLARK GKKVLIVERD
    60 70 80 90 100
    WAMPDRIVGE LMQPGGVRAL RSLGMIQSIN NIEAYPVTGY TVFFNGEQVD
    110 120 130 140 150
    IPYPYKADIP KVEKLKDLVK DGNDKVLEDS TIHIKDYEDD ERERGVAFVH
    160 170 180 190 200
    GRFLNNLRNI TAQEPNVTRV QGNCIEILKD EKNEVVGAKV DIDGRGKVEF
    210 220 230 240 250
    KAHLTFICDG IFSRFRKELH PDHVPTVGSS FVGMSLFNAK NPAPMHGHVI
    260 270 280 290 300
    LGSDHMPILV YQISPEETRI LCAYNSPKVP ADIKSWMIKD VQPFIPKSLR
    310 320 330 340 350
    PSFDEAVSQG KFRAMPNSYL PARQNDVTGM CVIGDALNMR HPLTGGGMTV
    360 370 380 390 400
    GLHDVVLLIK KIGDLDFSDR EKVLDELLDY HFERKSYDSV INVLSVALYS
    410 420 430 440 450
    LFAADSDNLK ALQKGCFKYF QRGGDCVNKP VEFLSGVLPK PLQLTRVFFA
    460 470 480 490
    VAFYTIYLNM EERGFLGLPM ALLEGIMILI TAIRVFTPFL FGELIG
    Length:496
    Mass (Da):55,126
    Last modified:October 1, 1996 - v2
    Checksum:iBBEFD766634855E8
    GO

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural varianti251L → F in strain: A2-M8; confers resistance to the allylamine antifungal terbinafine. 1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M64994 Genomic DNA. Translation: AAA34592.1.
    Z72960 Genomic DNA. Translation: CAA97201.1.
    BK006941 Genomic DNA. Translation: DAA08271.1.
    PIRiS64489.
    RefSeqiNP_011691.1. NM_001181304.1.

    Genome annotation databases

    EnsemblFungiiYGR175C; YGR175C; YGR175C.
    GeneIDi853086.
    KEGGisce:YGR175C.

    Similar proteinsi

    Entry informationi

    Entry nameiERG1_YEAST
    AccessioniPrimary (citable) accession number: P32476
    Secondary accession number(s): D6VUW0
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1996
    Last modified: October 25, 2017
    This is version 169 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names