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Reviewed, UniProtKB/Swiss-Prot P32476 (ERG1_YEAST)

Last modified June 16, 2009. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Squalene monooxygenase
    EC=1.14.99.7
Alternative name(s):
    Squalene epoxidase
      Short name=SE
Gene names
Name: ERG1
Ordered Locus Names: YGR175C
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length496 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway.

Catalytic activity

Squalene + AH2 + O2 = (S)-squalene-2,3-epoxide + A + H2O.

Cofactor

FAD.

Enzyme regulation

Inhibited by the allylamine antimycotic drugs.

Pathway

Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl-PP: step 2/3.

Subunit structure

Interacts with ERG28. Ref.6

Subcellular location

Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.

Miscellaneous

Present with 65400 molecules/cell in log phase SD medium. Ref.3

Sequence similarities

Belongs to the squalene monooxygenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 496496Squalene monooxygenase
PRO_0000209851

Regions

Topological domain1 – 1616Cytoplasmic Potential
Transmembrane17 – 3721 Potential
Topological domain38 – 474437Lumenal Potential
Transmembrane475 – 49521 Potential
Topological domain4961Cytoplasmic Potential
Nucleotide binding21 – 4828FAD Potential

Amino acid modifications

Cross-link284Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.5
Cross-link311Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.4

Natural variations

Natural variant2511L → F in strain: A2-M8; confers resistance to the allylamine antifungal terbinafine.

Sequences

Sequence LengthMass (Da)Tools
P32476-1 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: BBEFD766634855E8

FASTA49655,126
        10         20         30         40         50         60 
MSAVNVAPEL INADNTITYD AIVIGAGVIG PCVATGLARK GKKVLIVERD WAMPDRIVGE 

        70         80         90        100        110        120 
LMQPGGVRAL RSLGMIQSIN NIEAYPVTGY TVFFNGEQVD IPYPYKADIP KVEKLKDLVK 

       130        140        150        160        170        180 
DGNDKVLEDS TIHIKDYEDD ERERGVAFVH GRFLNNLRNI TAQEPNVTRV QGNCIEILKD 

       190        200        210        220        230        240 
EKNEVVGAKV DIDGRGKVEF KAHLTFICDG IFSRFRKELH PDHVPTVGSS FVGMSLFNAK 

       250        260        270        280        290        300 
NPAPMHGHVI LGSDHMPILV YQISPEETRI LCAYNSPKVP ADIKSWMIKD VQPFIPKSLR 

       310        320        330        340        350        360 
PSFDEAVSQG KFRAMPNSYL PARQNDVTGM CVIGDALNMR HPLTGGGMTV GLHDVVLLIK 

       370        380        390        400        410        420 
KIGDLDFSDR EKVLDELLDY HFERKSYDSV INVLSVALYS LFAADSDNLK ALQKGCFKYF 

       430        440        450        460        470        480 
QRGGDCVNKP VEFLSGVLPK PLQLTRVFFA VAFYTIYLNM EERGFLGLPM ALLEGIMILI 

       490 
TAIRVFTPFL FGELIG 

« Hide

References

« Hide 'large scale' references
[1]"The gene encoding squalene epoxidase from Saccharomyces cerevisiae: cloning and characterization."
Jandrositz A., Turnowsky F., Hoegenauer G.
Gene 107:155-160(1991) [PubMed: 1743514] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: A2-M8.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed: 9169869] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]"A proteomics approach to understanding protein ubiquitination."
Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P.
Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-311, MASS SPECTROMETRY.
[5]"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
Hitchcock A.L., Auld K., Gygi S.P., Silver P.A.
Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed: 14557538] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-284, MASS SPECTROMETRY.
[6]"Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex."
Mo C., Bard M.
J. Lipid Res. 46:1991-1998(2005) [PubMed: 15995173] [Abstract]
Cited for: INTERACTION WITH ERG28.
[7]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

M64994 Genomic DNA. Translation: AAA34592.1.
Z72960 Genomic DNA. Translation: CAA97201.1.
PIRS64489.
RefSeqNP_011691.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:6325N.
IntActP32476. 34 interactions.

Proteomic databases

PeptideAtlasP32476.

Genome annotation databases

EnsemblYGR175C. Saccharomyces cerevisiae. [Contig view]
GeneID853086.
GenomeReviewsGene locus YGR175C in contig Y13135_GR.
KEGGsce:YGR175C.
NMPDRfig|4932.3.peg.2818.

Organism-specific databases

CYGDYGR175c.
SGDS000003407. ERG1.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP32476.
OMAP32476. IPYPLSE.

Enzyme and pathway databases

BioCycMetaCyc:YGR175C-MON.
BRENDA1.14.99.7. 250.

Gene expression databases

GermOnlineYGR175C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR006076. FAD-dep_OxRdtase.
IPR003042. Rng_hydrolase-like.
IPR013698. Squalene_epoxidase.
[Graphical view]
PfamPF01266. DAO. 1 hit.
PF08491. SE. 1 hit.
[Graphical view]
PRINTSPR00420. RNGMNOXGNASE.
ProtoNetSearch...

Other Resources

NextBio973064.

Entry information

Entry nameERG1_YEAST
AccessionPrimary (citable) accession number: P32476
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: June 16, 2009
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents