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P32476

- ERG1_YEAST

UniProt

P32476 - ERG1_YEAST

Protein

Squalene monooxygenase

Gene

ERG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway.

    Catalytic activityi

    Squalene + NADPH + O2 = (3S)-2,3-epoxy-2,3-dihydrosqualene + NADP+ + H2O.

    Cofactori

    FAD.

    Enzyme regulationi

    Inhibited by the allylamine antimycotic drugs.

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi21 – 4828FADSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. drug binding Source: SGD
    2. flavin adenine dinucleotide binding Source: InterPro
    3. squalene monooxygenase activity Source: SGD

    GO - Biological processi

    1. ergosterol biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:YGR175C-MONOMER.
    YEAST:YGR175C-MONOMER.
    ReactomeiREACT_188982. Activation of gene expression by SREBF (SREBP).
    UniPathwayiUPA00767; UER00752.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Squalene monooxygenase (EC:1.14.13.132)
    Alternative name(s):
    Squalene epoxidase
    Short name:
    SE
    Gene namesi
    Name:ERG1
    Ordered Locus Names:YGR175C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR175c.
    SGDiS000003407. ERG1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: SGD
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW
    4. lipid particle Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 496496Squalene monooxygenasePRO_0000209851Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki284 – 284Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
    Cross-linki311 – 311Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

    Keywords - PTMi

    Isopeptide bond, Ubl conjugation

    Proteomic databases

    MaxQBiP32476.
    PaxDbiP32476.
    PeptideAtlasiP32476.

    Expressioni

    Gene expression databases

    GenevestigatoriP32476.

    Interactioni

    Subunit structurei

    Interacts with ERG28.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CSG2P352061EBI-6545,EBI-2051140
    ERG25P530451EBI-6545,EBI-6506
    ERG4P253401EBI-6545,EBI-6558
    ERP1Q053591EBI-6545,EBI-6581
    ERP4Q124501EBI-6545,EBI-6598
    ERV25P548371EBI-6545,EBI-6642
    ERV29P533371EBI-6545,EBI-23662
    GOT1Q035541EBI-6545,EBI-2057821
    PIS1P061971EBI-6545,EBI-13458
    SNA3P143591EBI-6545,EBI-26122
    SWP1Q027951EBI-6545,EBI-12666
    YHR146WP388451EBI-6545,EBI-24770
    YVH1Q022561EBI-6545,EBI-14322

    Protein-protein interaction databases

    BioGridi33428. 40 interactions.
    DIPiDIP-6325N.
    IntActiP32476. 16 interactions.
    MINTiMINT-1324933.
    STRINGi4932.YGR175C.

    Structurei

    3D structure databases

    ProteinModelPortaliP32476.
    SMRiP32476. Positions 20-50, 166-213.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1616CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini38 – 474437LumenalSequence AnalysisAdd
    BLAST
    Topological domaini496 – 4961CytoplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei17 – 3721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei475 – 49521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the squalene monooxygenase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0654.
    GeneTreeiENSGT00390000011759.
    HOGENOMiHOG000174713.
    KOiK00511.
    OMAiFITLANR.
    OrthoDBiEOG7KM62S.

    Family and domain databases

    InterProiIPR003042. Rng_hydrolase-like.
    IPR013698. Squalene_epoxidase.
    [Graphical view]
    PfamiPF08491. SE. 1 hit.
    [Graphical view]
    PRINTSiPR00420. RNGMNOXGNASE.

    Sequencei

    Sequence statusi: Complete.

    P32476-1 [UniParc]FASTAAdd to Basket

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    MSAVNVAPEL INADNTITYD AIVIGAGVIG PCVATGLARK GKKVLIVERD    50
    WAMPDRIVGE LMQPGGVRAL RSLGMIQSIN NIEAYPVTGY TVFFNGEQVD 100
    IPYPYKADIP KVEKLKDLVK DGNDKVLEDS TIHIKDYEDD ERERGVAFVH 150
    GRFLNNLRNI TAQEPNVTRV QGNCIEILKD EKNEVVGAKV DIDGRGKVEF 200
    KAHLTFICDG IFSRFRKELH PDHVPTVGSS FVGMSLFNAK NPAPMHGHVI 250
    LGSDHMPILV YQISPEETRI LCAYNSPKVP ADIKSWMIKD VQPFIPKSLR 300
    PSFDEAVSQG KFRAMPNSYL PARQNDVTGM CVIGDALNMR HPLTGGGMTV 350
    GLHDVVLLIK KIGDLDFSDR EKVLDELLDY HFERKSYDSV INVLSVALYS 400
    LFAADSDNLK ALQKGCFKYF QRGGDCVNKP VEFLSGVLPK PLQLTRVFFA 450
    VAFYTIYLNM EERGFLGLPM ALLEGIMILI TAIRVFTPFL FGELIG 496
    Length:496
    Mass (Da):55,126
    Last modified:October 1, 1996 - v2
    Checksum:iBBEFD766634855E8
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti251 – 2511L → F in strain: A2-M8; confers resistance to the allylamine antifungal terbinafine.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64994 Genomic DNA. Translation: AAA34592.1.
    Z72960 Genomic DNA. Translation: CAA97201.1.
    BK006941 Genomic DNA. Translation: DAA08271.1.
    PIRiS64489.
    RefSeqiNP_011691.1. NM_001181304.1.

    Genome annotation databases

    EnsemblFungiiYGR175C; YGR175C; YGR175C.
    GeneIDi853086.
    KEGGisce:YGR175C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64994 Genomic DNA. Translation: AAA34592.1 .
    Z72960 Genomic DNA. Translation: CAA97201.1 .
    BK006941 Genomic DNA. Translation: DAA08271.1 .
    PIRi S64489.
    RefSeqi NP_011691.1. NM_001181304.1.

    3D structure databases

    ProteinModelPortali P32476.
    SMRi P32476. Positions 20-50, 166-213.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33428. 40 interactions.
    DIPi DIP-6325N.
    IntActi P32476. 16 interactions.
    MINTi MINT-1324933.
    STRINGi 4932.YGR175C.

    Proteomic databases

    MaxQBi P32476.
    PaxDbi P32476.
    PeptideAtlasi P32476.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR175C ; YGR175C ; YGR175C .
    GeneIDi 853086.
    KEGGi sce:YGR175C.

    Organism-specific databases

    CYGDi YGR175c.
    SGDi S000003407. ERG1.

    Phylogenomic databases

    eggNOGi COG0654.
    GeneTreei ENSGT00390000011759.
    HOGENOMi HOG000174713.
    KOi K00511.
    OMAi FITLANR.
    OrthoDBi EOG7KM62S.

    Enzyme and pathway databases

    UniPathwayi UPA00767 ; UER00752 .
    BioCyci MetaCyc:YGR175C-MONOMER.
    YEAST:YGR175C-MONOMER.
    Reactomei REACT_188982. Activation of gene expression by SREBF (SREBP).

    Miscellaneous databases

    NextBioi 973064.
    PROi P32476.

    Gene expression databases

    Genevestigatori P32476.

    Family and domain databases

    InterProi IPR003042. Rng_hydrolase-like.
    IPR013698. Squalene_epoxidase.
    [Graphical view ]
    Pfami PF08491. SE. 1 hit.
    [Graphical view ]
    PRINTSi PR00420. RNGMNOXGNASE.
    ProtoNeti Search...

    Publicationsi

    1. "The gene encoding squalene epoxidase from Saccharomyces cerevisiae: cloning and characterization."
      Jandrositz A., Turnowsky F., Hoegenauer G.
      Gene 107:155-160(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: A2-M8.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-311.
      Strain: SUB592.
    6. "A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
      Hitchcock A.L., Auld K., Gygi S.P., Silver P.A.
      Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-284.
    7. "Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex."
      Mo C., Bard M.
      J. Lipid Res. 46:1991-1998(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ERG28.
    8. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiERG1_YEAST
    AccessioniPrimary (citable) accession number: P32476
    Secondary accession number(s): D6VUW0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 138 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 65400 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3