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Protein

Protein disulfide-isomerase EUG1

Gene

EUG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably interacts with nascent polypeptides in the endoplasmic reticulum. It is an essential gene only in the absence of PDI. Its native disulfide isomerase activity is very low.1 Publication

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

GO - Molecular functioni

  • protein disulfide isomerase activity Source: SGD
  • protein disulfide oxidoreductase activity Source: SGD
  • protein-disulfide reductase (glutathione) activity Source: SGD
  • unfolded protein binding Source: SGD

GO - Biological processi

  • cell redox homeostasis Source: InterPro
  • protein folding Source: SGD
  • response to endoplasmic reticulum stress Source: GO_Central

Keywordsi

Molecular functionIsomerase

Enzyme and pathway databases

BioCyciYEAST:YDR518W-MONOMER.
ReactomeiR-SCE-114608. Platelet degranulation.
R-SCE-3299685. Detoxification of Reactive Oxygen Species.
R-SCE-6798695. Neutrophil degranulation.
R-SCE-901042. Calnexin/calreticulin cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase EUG1 (EC:5.3.4.1)
Short name:
PDI
Alternative name(s):
Endoplasmic reticulum protein EUG1
Gene namesi
Name:EUG1
Ordered Locus Names:YDR518W
ORF Names:D9719.23
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR518W.
SGDiS000002926. EUG1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi65S → C: Increases PDI activity. 1 Publication1
Mutagenesisi408S → C: Increases PDI activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000003421930 – 517Protein disulfide-isomerase EUG1Add BLAST488

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi159N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi174N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi207N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi293N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi462N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

May have O-linked mannose residues.

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP32474.
PRIDEiP32474.

PTM databases

iPTMnetiP32474.

Interactioni

Subunit structurei

Interacts with EPS1.1 Publication

GO - Molecular functioni

  • unfolded protein binding Source: SGD

Protein-protein interaction databases

BioGridi32569. 152 interactors.
IntActiP32474. 1 interactor.
MINTiMINT-4811793.
STRINGi4932.YDR518W.

Structurei

3D structure databases

ProteinModelPortaliP32474.
SMRiP32474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 141Thioredoxin 1PROSITE-ProRule annotationAdd BLAST112
Domaini355 – 487Thioredoxin 2PROSITE-ProRule annotationAdd BLAST133

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi514 – 517Prevents secretion from ER4

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

GeneTreeiENSGT00860000133691.
HOGENOMiHOG000162459.
InParanoidiP32474.
KOiK09580.
OMAiYRAATQF.
OrthoDBiEOG092C3SRA.

Family and domain databases

InterProiView protein in InterPro
IPR005792. Prot_disulphide_isomerase.
IPR036249. Thioredoxin-like_sf.
IPR013766. Thioredoxin_domain.
PfamiView protein in Pfam
PF00085. Thioredoxin. 2 hits.
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
PROSITEiView protein in PROSITE
PS00014. ER_TARGET. 1 hit.
PS51352. THIOREDOXIN_2. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32474-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVTTRFISA IVSFCLFASF TLAENSARAT PGSDLLVLTE KKFKSFIESH
60 70 80 90 100
PLVLVEFFAP WCLHSQILRP HLEEAASILK EHNVPVVQID CEANSMVCLQ
110 120 130 140 150
QTINTYPTLK IFKNGRIFDG QVYRGVKITD EITQYMIQLY EASVIYLNSE
160 170 180 190 200
DEIQPYLENA TLPVVINRGL TGLNETYQEV ALDLAEDYVF LSLLDSEDKS
210 220 230 240 250
LSIHLPNTTE PILFDGNVDS LVGNSVALTQ WLKVVILPYF TDIEPDLFPK
260 270 280 290 300
YISSNLPLAY FFYTSEEELE DYTDLFTQLG KENRGQINFI ALNSTMFPHH
310 320 330 340 350
VRFLNMREQF PLFAIHNMIN NLKYGLPQLP EEEYAKLEKP QPLDRDMIVQ
360 370 380 390 400
LVKDYREGTA KPIVKSEEIP KEQKSNVYKI VGKTHDDIVH DDDKDVLVKY
410 420 430 440 450
YATWCIHSKR FAPIYEEIAN VLASDESVRD KILIAEVDSG ANDILSFPVT
460 470 480 490 500
GYPTIALYPA GNNSKPIIFN KIRNLEDVFE FIKESGTHHI DGQAIYDKLH
510
QAKDSEVSTE DTVHDEL
Length:517
Mass (Da):58,982
Last modified:October 1, 1993 - v1
Checksum:i5F78AD770A6A4083
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti364V → G in AAT92989 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84796 Unassigned DNA. Translation: AAA18226.1.
U33057 Genomic DNA. Translation: AAB64959.1.
AY692970 Genomic DNA. Translation: AAT92989.1.
BK006938 Genomic DNA. Translation: DAA12349.1.
PIRiA44483.
RefSeqiNP_010806.1. NM_001180826.1.

Genome annotation databases

EnsemblFungiiYDR518W; YDR518W; YDR518W.
GeneIDi852130.
KEGGisce:YDR518W.

Similar proteinsi

Entry informationi

Entry nameiEUG1_YEAST
AccessioniPrimary (citable) accession number: P32474
Secondary accession number(s): D6VTD9, E9P901
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 22, 2017
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names