Protein disulfide-isomerase EUG1 precursor - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P32474 (EUG1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 116. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein disulfide-isomerase EUG1
Short name=PDI
EC=5.3.4.1

Alternative name(s):
Endoplasmic reticulum protein EUG1

Gene names

Name:	EUG1
Ordered Locus Names:	YDR518W
ORF Names:	D9719.23

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	517 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably interacts with nascent polypeptides in the endoplasmic reticulum. It is an essential gene only in the absence of PDI. Its native disulfide isomerase activity is very low. Ref.6
Catalytic activity	Catalyzes the rearrangement of -S-S- bonds in proteins.
Subunit structure	Interacts with EPS1. Ref.6
Subcellular location	Endoplasmic reticulum lumen.
Post-translational modification	May have O-linked mannose residues.
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 2 thioredoxin domains.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Redox-active center Repeat Signal
Molecular function	Isomerase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum Inferred from direct assay PubMed 11914276. Source: SGD endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein disulfide isomerase activity Inferred from direct assay Ref.6. Source: SGD protein disulfide oxidoreductase activity Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

29

Potential

Chain

488

Protein disulfide-isomerase EUG1

PRO_0000034219

Regions

Domain

112

Thioredoxin 1

Domain

133

Thioredoxin 2

Motif

4

Prevents secretion from ER

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis

1

S → C: Increases PDI activity. Ref.5

Mutagenesis

1

S → C: Increases PDI activity. Ref.5

Sequence conflict

1

V → G in AAT92989. Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

P32474 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 5F78AD770A6A4083
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517

58,982

        10         20         30         40         50         60 
MQVTTRFISA IVSFCLFASF TLAENSARAT PGSDLLVLTE KKFKSFIESH PLVLVEFFAP 

        70         80         90        100        110        120 
WCLHSQILRP HLEEAASILK EHNVPVVQID CEANSMVCLQ QTINTYPTLK IFKNGRIFDG 

       130        140        150        160        170        180 
QVYRGVKITD EITQYMIQLY EASVIYLNSE DEIQPYLENA TLPVVINRGL TGLNETYQEV 

       190        200        210        220        230        240 
ALDLAEDYVF LSLLDSEDKS LSIHLPNTTE PILFDGNVDS LVGNSVALTQ WLKVVILPYF 

       250        260        270        280        290        300 
TDIEPDLFPK YISSNLPLAY FFYTSEEELE DYTDLFTQLG KENRGQINFI ALNSTMFPHH 

       310        320        330        340        350        360 
VRFLNMREQF PLFAIHNMIN NLKYGLPQLP EEEYAKLEKP QPLDRDMIVQ LVKDYREGTA 

       370        380        390        400        410        420 
KPIVKSEEIP KEQKSNVYKI VGKTHDDIVH DDDKDVLVKY YATWCIHSKR FAPIYEEIAN 

       430        440        450        460        470        480 
VLASDESVRD KILIAEVDSG ANDILSFPVT GYPTIALYPA GNNSKPIIFN KIRNLEDVFE 

       490        500        510 
FIKESGTHHI DGQAIYDKLH QAKDSEVSTE DTVHDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The yeast EUG1 gene encodes an endoplasmic reticulum protein that is functionally related to protein disulfide isomerase." Tachibana C., Stevens T.H. Mol. Cell. Biol. 12:4601-4611(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Mutation of yeast Eug1p CXXS active sites to CXXC results in a dramatic increase in protein disulphide isomerase activity." Noergaard P., Winther J.R. Biochem. J. 358:269-274(2001) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION OF PDI ACTIVITY, MUTAGENESIS OF SER-65 AND SER-408.
[6]	"Interactions among yeast protein-disulfide isomerase proteins and endoplasmic reticulum chaperone proteins influence their activities." Kimura T., Hosoda Y., Sato Y., Kitamura Y., Ikeda T., Horibe T., Kikuchi M. J. Biol. Chem. 280:31438-31441(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH EPS1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M84796 Unassigned DNA. Translation: AAA18226.1. U33057 Genomic DNA. Translation: AAB64959.1. AY692970 Genomic DNA. Translation: AAT92989.1. BK006938 Genomic DNA. Translation: DAA12349.1.
PIR	A44483.
RefSeq	NP_010806.1. NM_001180826.1.
3D structure databases
ProteinModelPortal	P32474.
SMR	P32474. Positions 24-496.
ModBase	Search...
Protein-protein interaction databases
IntAct	P32474. 1 interaction.
STRING	4932.YDR518W.
Proteomic databases
PaxDb	P32474.
PeptideAtlas	P32474.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR518W; YDR518W; YDR518W.
GeneID	852130.
KEGG	sce:YDR518W.
Organism-specific databases
CYGD	YDR518w.
SGD	S000002926. EUG1.
Phylogenomic databases
eggNOG	COG0526.
GeneTree	ENSGT00700000104218.
HOGENOM	HOG000162459.
KO	K09580.
OMA	DSGANDI.
OrthoDB	EOG4JHGQ4.
Gene expression databases
Genevestigator	P32474.
GermOnline	YDR518W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.30.10. 2 hits.
InterPro	IPR005792. Prot_disulphide_isomerase. IPR012336. Thioredoxin-like_fold. IPR013766. Thioredoxin_domain. [Graphical view]
Pfam	PF00085. Thioredoxin. 2 hits. [Graphical view]
SUPFAM	SSF52833. Thiordxn-like_fd. 4 hits.
TIGRFAMs	TIGR01130. ER_PDI_fam. 1 hit.
PROSITE	PS00014. ER_TARGET. 1 hit. PS51352. THIOREDOXIN_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	970528.

Entry information

Entry name

EUG1_YEAST

Accession

Primary (citable) accession number: P32474
Secondary accession number(s): D6VTD9, E9P901

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 1, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

SIMILARITY comments

Index of protein domains and families

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents