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Protein

Pyruvate dehydrogenase E1 component subunit beta, mitochondrial

Gene

PDB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.

Miscellaneous

Present with 9970 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei95Thiamine pyrophosphateBy similarity1

GO - Molecular functioni

  • pyruvate dehydrogenase (acetyl-transferring) activity Source: SGD

GO - Biological processi

  • acetyl-CoA biosynthetic process from pyruvate Source: SGD
  • glycolytic process Source: UniProtKB-KW

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandPyruvate, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciYEAST:YBR221C-MONOMER.
ReactomeiR-SCE-204174. Regulation of pyruvate dehydrogenase (PDH) complex.
R-SCE-389661. Glyoxylate metabolism and glycine degradation.
R-SCE-5362517. Signaling by Retinoic Acid.
R-SCE-70268. Pyruvate metabolism.
R-SCE-70895. Branched-chain amino acid catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (EC:1.2.4.1)
Alternative name(s):
Pyruvate dehydrogenase complex component E1 beta
Short name:
PDHE1-B
Gene namesi
Name:PDB1
Ordered Locus Names:YBR221C
ORF Names:YBR1511
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR221C.
SGDiS000000425. PDB1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 33Mitochondrion1 PublicationAdd BLAST33
ChainiPRO_000002046134 – 366Pyruvate dehydrogenase E1 component subunit beta, mitochondrialAdd BLAST333

Proteomic databases

MaxQBiP32473.
PRIDEiP32473.

2D gel databases

SWISS-2DPAGEiP32473.

PTM databases

iPTMnetiP32473.

Interactioni

Subunit structurei

Pyruvate dehydrogenase (E1) is a tetramer of 2 alpha and 2 beta subunits. Eukaryotic pyruvate dehydrogenase (PDH) complexes are organized as a core consisting of the oligomeric dihydrolipoamide acetyl-transferase (E2), around which are arranged multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3) and protein X (E3BP) bound by non-covalent bonds.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32918. 352 interactors.
DIPiDIP-1499N.
IntActiP32473. 39 interactors.
MINTiMINT-409839.
STRINGi4932.YBR221C.

Structurei

3D structure databases

ProteinModelPortaliP32473.
SMRiP32473.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00530000063423.
HOGENOMiHOG000281450.
InParanoidiP32473.
KOiK00162.
OMAiSRMRHHC.
OrthoDBiEOG092C45LK.

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
InterProiView protein in InterPro
IPR027110. PDHB.
IPR029061. THDP-binding.
IPR009014. Transketo_C/PFOR_II.
IPR005475. Transketolase-like_Pyr-bd.
IPR033248. Transketolase_C.
PANTHERiPTHR11624:SF89. PTHR11624:SF89. 1 hit.
PfamiView protein in Pfam
PF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
SMARTiView protein in SMART
SM00861. Transket_pyr. 1 hit.
SUPFAMiSSF52518. SSF52518. 1 hit.
SSF52922. SSF52922. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32473-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSRLPTSLA RNVARRAPTS FVRPSAAAAA LRFSSTKTMT VREALNSAMA
60 70 80 90 100
EELDRDDDVF LIGEEVAQYN GAYKVSKGLL DRFGERRVVD TPITEYGFTG
110 120 130 140 150
LAVGAALKGL KPIVEFMSFN FSMQAIDHVV NSAAKTHYMS GGTQKCQMVF
160 170 180 190 200
RGPNGAAVGV GAQHSQDFSP WYGSIPGLKV LVPYSAEDAR GLLKAAIRDP
210 220 230 240 250
NPVVFLENEL LYGESFEISE EALSPEFTLP YKAKIEREGT DISIVTYTRN
260 270 280 290 300
VQFSLEAAEI LQKKYGVSAE VINLRSIRPL DTEAIIKTVK KTNHLITVES
310 320 330 340 350
TFPSFGVGAE IVAQVMESEA FDYLDAPIQR VTGADVPTPY AKELEDFAFP
360
DTPTIVKAVK EVLSIE
Length:366
Mass (Da):40,054
Last modified:October 1, 1994 - v2
Checksum:i547FD49ED2CB8A97
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti160V → L in AAA34583 (PubMed:8433986).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98476 Genomic DNA. Translation: AAA34583.1.
Z36090 Genomic DNA. Translation: CAA85184.1.
AY692982 Genomic DNA. Translation: AAT93001.1.
BK006936 Genomic DNA. Translation: DAA07337.1.
PIRiS46097.
RefSeqiNP_009780.1. NM_001178569.1.

Genome annotation databases

EnsemblFungiiYBR221C; YBR221C; YBR221C.
GeneIDi852522.
KEGGisce:YBR221C.

Similar proteinsi

Entry informationi

Entry nameiODPB_YEAST
AccessioniPrimary (citable) accession number: P32473
Secondary accession number(s): D6VQL7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1994
Last modified: September 27, 2017
This is version 160 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names