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P32473

- ODPB_YEAST

UniProt

P32473 - ODPB_YEAST

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Protein

Pyruvate dehydrogenase E1 component subunit beta, mitochondrial

Gene

PDB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.

Catalytic activityi

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei95 – 951Thiamine pyrophosphateBy similarity

GO - Molecular functioni

  1. pyruvate dehydrogenase (acetyl-transferring) activity Source: SGD

GO - Biological processi

  1. acetyl-CoA biosynthetic process from pyruvate Source: SGD
  2. glycolytic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Pyruvate, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciYEAST:YBR221C-MONOMER.
ReactomeiREACT_259911. Pyruvate metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (EC:1.2.4.1)
Alternative name(s):
Pyruvate dehydrogenase complex component E1 beta
Short name:
PDHE1-B
Gene namesi
Name:PDB1
Ordered Locus Names:YBR221C
ORF Names:YBR1511
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR221c.
SGDiS000000425. PDB1.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial nucleoid Source: SGD
  2. mitochondrial pyruvate dehydrogenase complex Source: SGD
  3. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3333Mitochondrion1 PublicationAdd
BLAST
Chaini34 – 366333Pyruvate dehydrogenase E1 component subunit beta, mitochondrialPRO_0000020461Add
BLAST

Proteomic databases

MaxQBiP32473.
PaxDbiP32473.
PeptideAtlasiP32473.

2D gel databases

SWISS-2DPAGEP32473.

Expressioni

Gene expression databases

GenevestigatoriP32473.

Interactioni

Subunit structurei

Pyruvate dehydrogenase (E1) is a tetramer of 2 alpha and 2 beta subunits. Eukaryotic pyruvate dehydrogenase (PDH) complexes are organized as a core consisting of the oligomeric dihydrolipoamide acetyl-transferase (E2), around which are arranged multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3) and protein X (E3BP) bound by non-covalent bonds.

Protein-protein interaction databases

BioGridi32918. 161 interactions.
DIPiDIP-1499N.
IntActiP32473. 34 interactions.
MINTiMINT-409839.
STRINGi4932.YBR221C.

Structurei

3D structure databases

ProteinModelPortaliP32473.
SMRiP32473. Positions 39-365.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0022.
GeneTreeiENSGT00530000063423.
HOGENOMiHOG000281450.
InParanoidiP32473.
KOiK00162.
OMAiDPKVFVM.
OrthoDBiEOG7GBG70.

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
3.40.50.970. 1 hit.
InterProiIPR027110. PDHB.
IPR029061. THDP-binding.
IPR009014. Transketo_C/Pyr-ferredox_oxred.
IPR005475. Transketolase-like_Pyr-bd.
IPR005476. Transketolase_C.
[Graphical view]
PANTHERiPTHR11624:SF56. PTHR11624:SF56. 1 hit.
PfamiPF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
[Graphical view]
SMARTiSM00861. Transket_pyr. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 1 hit.
SSF52922. SSF52922. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32473-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFSRLPTSLA RNVARRAPTS FVRPSAAAAA LRFSSTKTMT VREALNSAMA
60 70 80 90 100
EELDRDDDVF LIGEEVAQYN GAYKVSKGLL DRFGERRVVD TPITEYGFTG
110 120 130 140 150
LAVGAALKGL KPIVEFMSFN FSMQAIDHVV NSAAKTHYMS GGTQKCQMVF
160 170 180 190 200
RGPNGAAVGV GAQHSQDFSP WYGSIPGLKV LVPYSAEDAR GLLKAAIRDP
210 220 230 240 250
NPVVFLENEL LYGESFEISE EALSPEFTLP YKAKIEREGT DISIVTYTRN
260 270 280 290 300
VQFSLEAAEI LQKKYGVSAE VINLRSIRPL DTEAIIKTVK KTNHLITVES
310 320 330 340 350
TFPSFGVGAE IVAQVMESEA FDYLDAPIQR VTGADVPTPY AKELEDFAFP
360
DTPTIVKAVK EVLSIE
Length:366
Mass (Da):40,054
Last modified:October 1, 1994 - v2
Checksum:i547FD49ED2CB8A97
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti160 – 1601V → L in AAA34583. (PubMed:8433986)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98476 Genomic DNA. Translation: AAA34583.1.
Z36090 Genomic DNA. Translation: CAA85184.1.
AY692982 Genomic DNA. Translation: AAT93001.1.
BK006936 Genomic DNA. Translation: DAA07337.1.
PIRiS46097.
RefSeqiNP_009780.1. NM_001178569.1.

Genome annotation databases

EnsemblFungiiYBR221C; YBR221C; YBR221C.
GeneIDi852522.
KEGGisce:YBR221C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98476 Genomic DNA. Translation: AAA34583.1 .
Z36090 Genomic DNA. Translation: CAA85184.1 .
AY692982 Genomic DNA. Translation: AAT93001.1 .
BK006936 Genomic DNA. Translation: DAA07337.1 .
PIRi S46097.
RefSeqi NP_009780.1. NM_001178569.1.

3D structure databases

ProteinModelPortali P32473.
SMRi P32473. Positions 39-365.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32918. 161 interactions.
DIPi DIP-1499N.
IntActi P32473. 34 interactions.
MINTi MINT-409839.
STRINGi 4932.YBR221C.

2D gel databases

SWISS-2DPAGE P32473.

Proteomic databases

MaxQBi P32473.
PaxDbi P32473.
PeptideAtlasi P32473.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR221C ; YBR221C ; YBR221C .
GeneIDi 852522.
KEGGi sce:YBR221C.

Organism-specific databases

CYGDi YBR221c.
SGDi S000000425. PDB1.

Phylogenomic databases

eggNOGi COG0022.
GeneTreei ENSGT00530000063423.
HOGENOMi HOG000281450.
InParanoidi P32473.
KOi K00162.
OMAi DPKVFVM.
OrthoDBi EOG7GBG70.

Enzyme and pathway databases

BioCyci YEAST:YBR221C-MONOMER.
Reactomei REACT_259911. Pyruvate metabolism.

Miscellaneous databases

NextBioi 971562.
PROi P32473.

Gene expression databases

Genevestigatori P32473.

Family and domain databases

Gene3Di 3.40.50.920. 1 hit.
3.40.50.970. 1 hit.
InterProi IPR027110. PDHB.
IPR029061. THDP-binding.
IPR009014. Transketo_C/Pyr-ferredox_oxred.
IPR005475. Transketolase-like_Pyr-bd.
IPR005476. Transketolase_C.
[Graphical view ]
PANTHERi PTHR11624:SF56. PTHR11624:SF56. 1 hit.
Pfami PF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
[Graphical view ]
SMARTi SM00861. Transket_pyr. 1 hit.
[Graphical view ]
SUPFAMi SSF52518. SSF52518. 1 hit.
SSF52922. SSF52922. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of PDH beta 1, the structural gene for the pyruvate dehydrogenase beta subunit from Saccharomyces cerevisiae."
    Miran S.G., Lawson J.E., Reed L.J.
    Proc. Natl. Acad. Sci. U.S.A. 90:1252-1256(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 34-54 AND 206-219.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.

Entry informationi

Entry nameiODPB_YEAST
AccessioniPrimary (citable) accession number: P32473
Secondary accession number(s): D6VQL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1994
Last modified: November 26, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 9970 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3