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P32473

- ODPB_YEAST

UniProt

P32473 - ODPB_YEAST

Protein

Pyruvate dehydrogenase E1 component subunit beta, mitochondrial

Gene

PDB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 2 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.

    Catalytic activityi

    Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

    Cofactori

    Thiamine pyrophosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei95 – 951Thiamine pyrophosphateBy similarity

    GO - Molecular functioni

    1. pyruvate dehydrogenase (acetyl-transferring) activity Source: SGD

    GO - Biological processi

    1. acetyl-CoA biosynthetic process from pyruvate Source: SGD
    2. glycolytic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Pyruvate, Thiamine pyrophosphate

    Enzyme and pathway databases

    BioCyciYEAST:YBR221C-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (EC:1.2.4.1)
    Alternative name(s):
    Pyruvate dehydrogenase complex component E1 beta
    Short name:
    PDHE1-B
    Gene namesi
    Name:PDB1
    Ordered Locus Names:YBR221C
    ORF Names:YBR1511
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR221c.
    SGDiS000000425. PDB1.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial nucleoid Source: SGD
    2. mitochondrial pyruvate dehydrogenase complex Source: SGD
    3. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3333Mitochondrion1 PublicationAdd
    BLAST
    Chaini34 – 366333Pyruvate dehydrogenase E1 component subunit beta, mitochondrialPRO_0000020461Add
    BLAST

    Proteomic databases

    MaxQBiP32473.
    PaxDbiP32473.
    PeptideAtlasiP32473.

    2D gel databases

    SWISS-2DPAGEP32473.

    Expressioni

    Gene expression databases

    GenevestigatoriP32473.

    Interactioni

    Subunit structurei

    Pyruvate dehydrogenase (E1) is a tetramer of 2 alpha and 2 beta subunits. Eukaryotic pyruvate dehydrogenase (PDH) complexes are organized as a core consisting of the oligomeric dihydrolipoamide acetyl-transferase (E2), around which are arranged multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3) and protein X (E3BP) bound by non-covalent bonds.

    Protein-protein interaction databases

    BioGridi32918. 159 interactions.
    DIPiDIP-1499N.
    IntActiP32473. 34 interactions.
    MINTiMINT-409839.
    STRINGi4932.YBR221C.

    Structurei

    3D structure databases

    ProteinModelPortaliP32473.
    SMRiP32473. Positions 39-365.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0022.
    GeneTreeiENSGT00530000063423.
    HOGENOMiHOG000281450.
    KOiK00162.
    OMAiDPKVFVM.
    OrthoDBiEOG7GBG70.

    Family and domain databases

    Gene3Di3.40.50.920. 1 hit.
    3.40.50.970. 1 hit.
    InterProiIPR027110. PDHB.
    IPR029061. THDP-binding.
    IPR009014. Transketo_C/Pyr-ferredox_oxred.
    IPR005475. Transketolase-like_Pyr-bd.
    IPR005476. Transketolase_C.
    [Graphical view]
    PANTHERiPTHR11624:SF56. PTHR11624:SF56. 1 hit.
    PfamiPF02779. Transket_pyr. 1 hit.
    PF02780. Transketolase_C. 1 hit.
    [Graphical view]
    SMARTiSM00861. Transket_pyr. 1 hit.
    [Graphical view]
    SUPFAMiSSF52518. SSF52518. 1 hit.
    SSF52922. SSF52922. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32473-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFSRLPTSLA RNVARRAPTS FVRPSAAAAA LRFSSTKTMT VREALNSAMA    50
    EELDRDDDVF LIGEEVAQYN GAYKVSKGLL DRFGERRVVD TPITEYGFTG 100
    LAVGAALKGL KPIVEFMSFN FSMQAIDHVV NSAAKTHYMS GGTQKCQMVF 150
    RGPNGAAVGV GAQHSQDFSP WYGSIPGLKV LVPYSAEDAR GLLKAAIRDP 200
    NPVVFLENEL LYGESFEISE EALSPEFTLP YKAKIEREGT DISIVTYTRN 250
    VQFSLEAAEI LQKKYGVSAE VINLRSIRPL DTEAIIKTVK KTNHLITVES 300
    TFPSFGVGAE IVAQVMESEA FDYLDAPIQR VTGADVPTPY AKELEDFAFP 350
    DTPTIVKAVK EVLSIE 366
    Length:366
    Mass (Da):40,054
    Last modified:October 1, 1994 - v2
    Checksum:i547FD49ED2CB8A97
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti160 – 1601V → L in AAA34583. (PubMed:8433986)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98476 Genomic DNA. Translation: AAA34583.1.
    Z36090 Genomic DNA. Translation: CAA85184.1.
    AY692982 Genomic DNA. Translation: AAT93001.1.
    BK006936 Genomic DNA. Translation: DAA07337.1.
    PIRiS46097.
    RefSeqiNP_009780.1. NM_001178569.1.

    Genome annotation databases

    EnsemblFungiiYBR221C; YBR221C; YBR221C.
    GeneIDi852522.
    KEGGisce:YBR221C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98476 Genomic DNA. Translation: AAA34583.1 .
    Z36090 Genomic DNA. Translation: CAA85184.1 .
    AY692982 Genomic DNA. Translation: AAT93001.1 .
    BK006936 Genomic DNA. Translation: DAA07337.1 .
    PIRi S46097.
    RefSeqi NP_009780.1. NM_001178569.1.

    3D structure databases

    ProteinModelPortali P32473.
    SMRi P32473. Positions 39-365.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32918. 159 interactions.
    DIPi DIP-1499N.
    IntActi P32473. 34 interactions.
    MINTi MINT-409839.
    STRINGi 4932.YBR221C.

    2D gel databases

    SWISS-2DPAGE P32473.

    Proteomic databases

    MaxQBi P32473.
    PaxDbi P32473.
    PeptideAtlasi P32473.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR221C ; YBR221C ; YBR221C .
    GeneIDi 852522.
    KEGGi sce:YBR221C.

    Organism-specific databases

    CYGDi YBR221c.
    SGDi S000000425. PDB1.

    Phylogenomic databases

    eggNOGi COG0022.
    GeneTreei ENSGT00530000063423.
    HOGENOMi HOG000281450.
    KOi K00162.
    OMAi DPKVFVM.
    OrthoDBi EOG7GBG70.

    Enzyme and pathway databases

    BioCyci YEAST:YBR221C-MONOMER.

    Miscellaneous databases

    NextBioi 971562.
    PROi P32473.

    Gene expression databases

    Genevestigatori P32473.

    Family and domain databases

    Gene3Di 3.40.50.920. 1 hit.
    3.40.50.970. 1 hit.
    InterProi IPR027110. PDHB.
    IPR029061. THDP-binding.
    IPR009014. Transketo_C/Pyr-ferredox_oxred.
    IPR005475. Transketolase-like_Pyr-bd.
    IPR005476. Transketolase_C.
    [Graphical view ]
    PANTHERi PTHR11624:SF56. PTHR11624:SF56. 1 hit.
    Pfami PF02779. Transket_pyr. 1 hit.
    PF02780. Transketolase_C. 1 hit.
    [Graphical view ]
    SMARTi SM00861. Transket_pyr. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52518. SSF52518. 1 hit.
    SSF52922. SSF52922. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of PDH beta 1, the structural gene for the pyruvate dehydrogenase beta subunit from Saccharomyces cerevisiae."
      Miran S.G., Lawson J.E., Reed L.J.
      Proc. Natl. Acad. Sci. U.S.A. 90:1252-1256(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 34-54 AND 206-219.
    2. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.

    Entry informationi

    Entry nameiODPB_YEAST
    AccessioniPrimary (citable) accession number: P32473
    Secondary accession number(s): D6VQL7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 131 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 9970 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3