Reviewed, UniProtKB/Swiss-Prot P32470 (CHI1_APHAL)

Names and origin

Protein names

Recommended name:
    Chitinase 1
    EC=3.2.1.14

Gene names

Name:

CHI1

Organism

Aphanocladium album

Taxonomic identifier

12942 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › mitosporic Ascomycota › Aphanocladium

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subcellular location	Secreted.
Sequence similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Cleavage on pair of basic residues Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Propeptide	23 – 34	12	Potential		PRO_0000011922
	Chain	35 – 423	389	Chitinase 1		PRO_0000011923
Sites
	Active site	171	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32470-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 55DFB25B73443F31	FASTA	423	46,059
	10 20 30 40 50 60 MLSFVKKSIA LVAALQAVTA LATPISSEAG VEKRGSGFAN AVYFTNWGIY GRNFQPADLP 70 80 90 100 110 120 ASEITHVLYS FMNVRADGTI FSGDTYADYE KHYAGDSWND VGTNAYGCVK QLYLLKKQNR 130 140 150 160 170 180 NMKVMLSIGG WTWSTNFPAA ASSAATRKTF AQSAVGFMKD WGFDGIDIDW EYPADATQAQ 190 200 210 220 230 240 NMVLLLQAVR SELDSYAAQY AKGHHFLLSI AAPAGPDNYN KLKFAELGKV LDYINLMAYD 250 260 270 280 290 300 YAGSWSNYTG HDANIYANPQ NPNATPYNTD DAVQAYINGG VPANKIVLGM PIYGRSFQQT 310 320 330 340 350 360 EGIGKPYNGI GSGSWENGIW DYKALPKAGA TVKCDDTAKG CYSYDPSTKE LISFDTPAMI 370 380 390 400 410 420 STKVSWLKGK GLGGTMFWEA SASKKGSDSL ISTSHQGLGS QDSTQNYLDY PNSKYDNIKK GMN

References

[1]	"Primary structure of a chitinase-encoding gene (chi1) from the filamentous fungus Aphanocladium album: similarity to bacterial chitinases." Blaiseau P.-L., Lafay J.-F. Gene 120:243-248(1992) [PubMed: 1398137] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ETHM 483.
[2]	"Cloning and expression of a chitinase gene from the hyperparasitic fungus Aphanocladium album." Blaiseau P.-L., Kunz C., Grison R., Bertheau Y., Brygoo Y. Curr. Genet. 21:61-66(1992) [PubMed: 1735126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 35-67, PROTEIN SEQUENCE OF 35-67.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X64104 Genomic DNA. Translation: CAA45468.1. S81303 Genomic DNA. Translation: AAB21333.1.
PIR	JQ1975. S26859.
3D structure databases
HSSP	HSSP built from PDB template 1D2K based on UniProtKB P54196.
ModBase	Search...
Protein family/group databases
CAZy	GH18. Glycoside Hydrolase Family 18.
Enzyme and pathway databases
BRENDA	3.2.1.14. 290904.
Family and domain databases
InterPro	IPR011583. Chitinase_II. IPR001223. Glyco_hydro18cat. IPR001579. Glyco_hydro_18_chit_AS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00704. Glyco_hydro_18. 1 hit. [Graphical view]
ProDom	PD000471. Chitinase_II. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00636. Glyco_18. 1 hit. [Graphical view]
PROSITE	PS01095. CHITINASE_18. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHI1_APHAL

Accession

Primary (citable) accession number: P32470

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)