Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Diphthine methyl ester synthase

Gene

DPH5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.2 Publications

Miscellaneous

2-[3-carboxy-3-(methylammonio)propyl]-L-histidine and the corresponding dimethyl compound can also act as acceptors.
Present with 13480 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

4 S-adenosyl-L-methionine + 2-((3S)-3-carboxy-3-aminopropyl)-L-histidine-[translation elongation factor 2] = 4 S-adenosyl-L-homocysteine + diphthine methyl ester-[translation elongation factor 2].1 Publication

Pathwayi: peptidyl-diphthamide biosynthesis

This protein is involved in the pathway peptidyl-diphthamide biosynthesis, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway peptidyl-diphthamide biosynthesis and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei85S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei88S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei164S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei222S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei247S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1

GO - Molecular functioni

  • diphthine synthase activity Source: SGD

GO - Biological processi

  • peptidyl-diphthamide biosynthetic process from peptidyl-histidine Source: SGD

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15582
YEAST:MONOMER-15582
ReactomeiR-SCE-5358493 Synthesis of diphthamide-EEF2
UniPathwayiUPA00559

Names & Taxonomyi

Protein namesi
Recommended name:
Diphthine methyl ester synthase (EC:2.1.1.3141 Publication)
Alternative name(s):
Diphthamide biosynthesis methyltransferase
Gene namesi
Name:DPH5
Ordered Locus Names:YLR172C
ORF Names:L9470.17
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR172C
SGDiS000004162 DPH5

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001561471 – 300Diphthine methyl ester synthaseAdd BLAST300

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei172PhosphoserineCombined sources1
Modified residuei298PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32469
PaxDbiP32469
PRIDEiP32469

PTM databases

iPTMnetiP32469

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SSA2P105923EBI-6095,EBI-8603

Protein-protein interaction databases

BioGridi31443, 171 interactors
DIPiDIP-4237N
IntActiP32469, 11 interactors
MINTiP32469
STRINGi4932.YLR172C

Structurei

3D structure databases

ProteinModelPortaliP32469
SMRiP32469
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni113 – 114S-adenosyl-L-methionine bindingBy similarity2

Sequence similaritiesi

Belongs to the diphthine synthase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000010568
HOGENOMiHOG000205302
InParanoidiP32469
KOiK00586
OMAiGLQNYRF
OrthoDBiEOG092C4D28

Family and domain databases

CDDicd11647 Diphthine_synthase, 1 hit
Gene3Di3.30.950.10, 1 hit
3.40.1010.10, 1 hit
HAMAPiMF_01084 Diphthine_synth, 1 hit
InterProiView protein in InterPro
IPR000878 4pyrrol_Mease
IPR035996 4pyrrol_Methylase_sf
IPR014777 4pyrrole_Mease_sub1
IPR014776 4pyrrole_Mease_sub2
IPR004551 Dphthn_synthase
PANTHERiPTHR10882:SF0 PTHR10882:SF0, 1 hit
PfamiView protein in Pfam
PF00590 TP_methylase, 1 hit
PIRSFiPIRSF036432 Diphthine_synth, 1 hit
SUPFAMiSSF53790 SSF53790, 1 hit
TIGRFAMsiTIGR00522 dph5, 1 hit

Sequencei

Sequence statusi: Complete.

P32469-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLYLIGLGLS YKSDITVRGL EAIKKCSRVY LEHYTSILMA ASQEELESYY
60 70 80 90 100
GKEIILADRE LVETGSKQIL NNADKEDVAF LVVGDPFGAT THTDLVLRAK
110 120 130 140 150
REAIPVEIIH NASVMNAVGA CGLQLYNFGQ TVSMVFFTDN WRPDSWYDKI
160 170 180 190 200
WENRKIGLHT LVLLDIKVKE QSIENMARGR LIYEPPRYMS IAQCCEQLLE
210 220 230 240 250
IEEKRGTKAY TPDTPAVAIS RLGSSSQSFK SGTISELANY DSGEPLHSLV
260 270 280 290 300
ILGRQCHELE LEYLLEFADD KEKFGKDVAN DQEYFKPAAW VPPTEDDSDE
Length:300
Mass (Da):33,847
Last modified:October 1, 1993 - v1
Checksum:i405411F6BF2E6DF9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti287P → L in AAS56273 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83375 Genomic DNA Translation: AAA34577.1
U17246 Genomic DNA Translation: AAB67469.1
AY557947 Genomic DNA Translation: AAS56273.1
X70279 Genomic DNA Translation: CAA49764.1
BK006945 Genomic DNA Translation: DAA09492.1
PIRiS30890
RefSeqiNP_013273.1, NM_001182059.1

Genome annotation databases

EnsemblFungiiYLR172C; YLR172C; YLR172C
GeneIDi850869
KEGGisce:YLR172C

Similar proteinsi

Entry informationi

Entry nameiDPH5_YEAST
AccessioniPrimary (citable) accession number: P32469
Secondary accession number(s): D6VYH6, Q6Q5L4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: March 28, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health