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Protein

Low-affinity glucose transporter HXT4

Gene

HXT4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Low-affinity glucose transporter. Can also transport xylose.1 Publication

Miscellaneous

Glucose transport is thought to be mediated by two kinetically distinct systems, a glucose-repressible high-affinity system and a constitutive low-affinity system.

Enzyme regulationi

Xylose uptake is strongly inhibited by glucose.

Kineticsi

  1. KM=170 mM for xylose uptake1 Publication
  1. Vmax=190 nmol/min/mg enzyme for xylose uptake1 Publication

GO - Molecular functioni

  • carbohydrate:proton symporter activity Source: GO_Central
  • fructose transmembrane transporter activity Source: SGD
  • glucose transmembrane transporter activity Source: SGD
  • mannose transmembrane transporter activity Source: SGD
  • pentose transmembrane transporter activity Source: SGD

GO - Biological processi

  • glucose import Source: GO_Central
  • hexose transmembrane transport Source: SGD

Keywordsi

Biological processSugar transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31139-MONOMER
ReactomeiR-SCE-189200 Cellular hexose transport
R-SCE-8856825 Cargo recognition for clathrin-mediated endocytosis

Protein family/group databases

TCDBi2.A.1.1.30 the major facilitator superfamily (mfs)

Names & Taxonomyi

Protein namesi
Recommended name:
Low-affinity glucose transporter HXT4
Alternative name(s):
Low-affinity glucose transporter LGT1
Gene namesi
Name:HXT4
Synonyms:LGT1, RAG1
Ordered Locus Names:YHR092C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR092C
SGDiS000001134 HXT4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 66CytoplasmicSequence analysisAdd BLAST66
Transmembranei67 – 87Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini88 – 122ExtracellularSequence analysisAdd BLAST35
Transmembranei123 – 143Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini144 – 149CytoplasmicSequence analysis6
Transmembranei150 – 170Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini171 – 180ExtracellularSequence analysis10
Transmembranei181 – 201Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini202 – 207CytoplasmicSequence analysis6
Transmembranei208 – 228Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini229 – 242ExtracellularSequence analysisAdd BLAST14
Transmembranei243 – 263Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini264 – 346CytoplasmicSequence analysisAdd BLAST83
Transmembranei347 – 363Helical; Name=7Sequence analysisAdd BLAST17
Topological domaini364 – 369ExtracellularSequence analysis6
Transmembranei370 – 387Helical; Name=8Sequence analysisAdd BLAST18
Topological domaini388 – 394CytoplasmicSequence analysis7
Transmembranei395 – 415Helical; Name=9Sequence analysisAdd BLAST21
Topological domaini416 – 437ExtracellularSequence analysisAdd BLAST22
Transmembranei438 – 458Helical; Name=10Sequence analysisAdd BLAST21
Topological domaini459 – 475CytoplasmicSequence analysisAdd BLAST17
Transmembranei476 – 496Helical; Name=11Sequence analysisAdd BLAST21
Topological domaini497ExtracellularSequence analysis1
Transmembranei498 – 518Helical; Name=12Sequence analysisAdd BLAST21
Topological domaini519 – 576CytoplasmicSequence analysisAdd BLAST58

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000503941 – 576Low-affinity glucose transporter HXT4Add BLAST576

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki45Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Glycosylationi425N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP32467
PaxDbiP32467
PRIDEiP32467

PTM databases

iPTMnetiP32467

Interactioni

Protein-protein interaction databases

BioGridi36527, 66 interactors
DIPiDIP-7908N
IntActiP32467, 11 interactors
STRINGi4932.YHR092C

Structurei

3D structure databases

ProteinModelPortaliP32467
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00910000144141
HOGENOMiHOG000202870
InParanoidiP32467
KOiK08139
OMAiVIGWSGP
OrthoDBiEOG092C1Q5W

Family and domain databases

CDDicd06174 MFS, 1 hit
InterProiView protein in InterPro
IPR020846 MFS_dom
IPR005828 MFS_sugar_transport-like
IPR036259 MFS_trans_sf
IPR003663 Sugar/inositol_transpt
IPR005829 Sugar_transporter_CS
PfamiView protein in Pfam
PF00083 Sugar_tr, 1 hit
PRINTSiPR00171 SUGRTRNSPORT
SUPFAMiSSF103473 SSF103473, 2 hits
TIGRFAMsiTIGR00879 SP, 1 hit
PROSITEiView protein in PROSITE
PS50850 MFS, 1 hit
PS00216 SUGAR_TRANSPORT_1, 1 hit
PS00217 SUGAR_TRANSPORT_2, 1 hit

Sequencei

Sequence statusi: Complete.

P32467-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEEAAYQED TAVQNTPADA LSPVESDSNS ALSTPSNKAE RDDMKDFDEN
60 70 80 90 100
HEESNNYVEI PKKPASAYVT VSICCLMVAF GGFVFGWDTG TISGFVAQTD
110 120 130 140 150
FIRRFGMKHH DGTYYLSKVR TGLIVSIFNI GCAIGGIILA KLGDMYGRKM
160 170 180 190 200
GLIVVVVIYI IGIIIQIASI NKWYQYFIGR IISGLGVGGI AVLSPMLISE
210 220 230 240 250
VSPKHIRGTL VSCYQLMITL GIFLGYCTNY GTKTYTNSVQ WRVPLGLGFA
260 270 280 290 300
WALFMIGGMT FVPESPRYLV EVGKIEEAKR SIALSNKVSA DDPAVMAEVE
310 320 330 340 350
VVQATVEAEK LAGNASWGEI FSTKTKVFQR LIMGAMIQSL QQLTGDNYFF
360 370 380 390 400
YYGTTVFTAV GLEDSFETSI VLGIVNFAST FVGIFLVERY GRRRCLLWGA
410 420 430 440 450
ASMTACMVVF ASVGVTRLWP NGKKNGSSKG AGNCMIVFTC FYLFCFATTW
460 470 480 490 500
APIPFVVNSE TFPLRVKSKC MAIAQACNWI WGFLIGFFTP FISGAIDFYY
510 520 530 540 550
GYVFMGCLVF SYFYVFFFVP ETKGLTLEEV NTLWEEGVLP WKSPSWVPPN
560 570
KRGTDYNADD LMHDDQPFYK KMFGKK
Length:576
Mass (Da):63,910
Last modified:October 1, 1993 - v1
Checksum:iF58E0060721E9EC7
GO

Sequence cautioni

The sequence AAB68932 differs from that shown. Reason: Frameshift at positions 547, 565 and 567.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81960 Genomic DNA Translation: AAA20997.1
X67321 Genomic DNA Translation: CAA47735.1
U00060 Genomic DNA Translation: AAB68932.1 Frameshift.
BK006934 Genomic DNA Translation: DAA06788.2
PIRiS46724
RefSeqiNP_011960.2, NM_001179222.2

Genome annotation databases

EnsemblFungiiYHR092C; YHR092C; YHR092C
GeneIDi856492
KEGGisce:YHR092C

Similar proteinsi

Entry informationi

Entry nameiHXT4_YEAST
AccessioniPrimary (citable) accession number: P32467
Secondary accession number(s): D3DL44
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

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