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Protein

Low-affinity glucose transporter HXT4

Gene

HXT4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Low-affinity glucose transporter. Can also transport xylose.1 Publication

Enzyme regulationi

Xylose uptake is strongly inhibited by glucose.

Kineticsi

  1. KM=170 mM for xylose uptake1 Publication
  1. Vmax=190 nmol/min/mg enzyme for xylose uptake1 Publication

GO - Molecular functioni

  • fructose transmembrane transporter activity Source: SGD
  • glucose transmembrane transporter activity Source: SGD
  • mannose transmembrane transporter activity Source: SGD
  • pentose transmembrane transporter activity Source: SGD

GO - Biological processi

  • fructose transport Source: GOC
  • glucose transmembrane transport Source: GOC
  • hexose transport Source: SGD
  • mannose transport Source: GOC
  • pentose transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Sugar transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31139-MONOMER.
ReactomeiR-SCE-428790. Facilitative Na+-independent glucose transporters.

Protein family/group databases

TCDBi2.A.1.1.30. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Low-affinity glucose transporter HXT4
Alternative name(s):
Low-affinity glucose transporter LGT1
Gene namesi
Name:HXT4
Synonyms:LGT1, RAG1
Ordered Locus Names:YHR092C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR092C.
SGDiS000001134. HXT4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6666CytoplasmicSequence analysisAdd
BLAST
Transmembranei67 – 8721Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini88 – 12235ExtracellularSequence analysisAdd
BLAST
Transmembranei123 – 14321Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini144 – 1496CytoplasmicSequence analysis
Transmembranei150 – 17021Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini171 – 18010ExtracellularSequence analysis
Transmembranei181 – 20121Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini202 – 2076CytoplasmicSequence analysis
Transmembranei208 – 22821Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini229 – 24214ExtracellularSequence analysisAdd
BLAST
Transmembranei243 – 26321Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini264 – 34683CytoplasmicSequence analysisAdd
BLAST
Transmembranei347 – 36317Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini364 – 3696ExtracellularSequence analysis
Transmembranei370 – 38718Helical; Name=8Sequence analysisAdd
BLAST
Topological domaini388 – 3947CytoplasmicSequence analysis
Transmembranei395 – 41521Helical; Name=9Sequence analysisAdd
BLAST
Topological domaini416 – 43722ExtracellularSequence analysisAdd
BLAST
Transmembranei438 – 45821Helical; Name=10Sequence analysisAdd
BLAST
Topological domaini459 – 47517CytoplasmicSequence analysisAdd
BLAST
Transmembranei476 – 49621Helical; Name=11Sequence analysisAdd
BLAST
Topological domaini497 – 4971ExtracellularSequence analysis
Transmembranei498 – 51821Helical; Name=12Sequence analysisAdd
BLAST
Topological domaini519 – 57658CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 576576Low-affinity glucose transporter HXT4PRO_0000050394Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki45 – 45Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Glycosylationi425 – 4251N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP32467.

Interactioni

Protein-protein interaction databases

BioGridi36527. 25 interactions.
DIPiDIP-7908N.
IntActiP32467. 11 interactions.

Structurei

3D structure databases

ProteinModelPortaliP32467.
SMRiP32467. Positions 76-535.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00720000108754.
HOGENOMiHOG000202870.
InParanoidiP32467.
KOiK08139.
OMAiEVNTLWE.
OrthoDBiEOG73JM4H.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport_like.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32467-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEEAAYQED TAVQNTPADA LSPVESDSNS ALSTPSNKAE RDDMKDFDEN
60 70 80 90 100
HEESNNYVEI PKKPASAYVT VSICCLMVAF GGFVFGWDTG TISGFVAQTD
110 120 130 140 150
FIRRFGMKHH DGTYYLSKVR TGLIVSIFNI GCAIGGIILA KLGDMYGRKM
160 170 180 190 200
GLIVVVVIYI IGIIIQIASI NKWYQYFIGR IISGLGVGGI AVLSPMLISE
210 220 230 240 250
VSPKHIRGTL VSCYQLMITL GIFLGYCTNY GTKTYTNSVQ WRVPLGLGFA
260 270 280 290 300
WALFMIGGMT FVPESPRYLV EVGKIEEAKR SIALSNKVSA DDPAVMAEVE
310 320 330 340 350
VVQATVEAEK LAGNASWGEI FSTKTKVFQR LIMGAMIQSL QQLTGDNYFF
360 370 380 390 400
YYGTTVFTAV GLEDSFETSI VLGIVNFAST FVGIFLVERY GRRRCLLWGA
410 420 430 440 450
ASMTACMVVF ASVGVTRLWP NGKKNGSSKG AGNCMIVFTC FYLFCFATTW
460 470 480 490 500
APIPFVVNSE TFPLRVKSKC MAIAQACNWI WGFLIGFFTP FISGAIDFYY
510 520 530 540 550
GYVFMGCLVF SYFYVFFFVP ETKGLTLEEV NTLWEEGVLP WKSPSWVPPN
560 570
KRGTDYNADD LMHDDQPFYK KMFGKK
Length:576
Mass (Da):63,910
Last modified:October 1, 1993 - v1
Checksum:iF58E0060721E9EC7
GO

Sequence cautioni

The sequence AAB68932.1 differs from that shown. Reason: Frameshift at positions 547, 565 and 567. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81960 Genomic DNA. Translation: AAA20997.1.
X67321 Genomic DNA. Translation: CAA47735.1.
U00060 Genomic DNA. Translation: AAB68932.1. Frameshift.
BK006934 Genomic DNA. Translation: DAA06788.2.
PIRiS46724.
RefSeqiNP_011960.2. NM_001179222.2.

Genome annotation databases

EnsemblFungiiYHR092C; YHR092C; YHR092C.
GeneIDi856492.
KEGGisce:YHR092C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81960 Genomic DNA. Translation: AAA20997.1.
X67321 Genomic DNA. Translation: CAA47735.1.
U00060 Genomic DNA. Translation: AAB68932.1. Frameshift.
BK006934 Genomic DNA. Translation: DAA06788.2.
PIRiS46724.
RefSeqiNP_011960.2. NM_001179222.2.

3D structure databases

ProteinModelPortaliP32467.
SMRiP32467. Positions 76-535.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36527. 25 interactions.
DIPiDIP-7908N.
IntActiP32467. 11 interactions.

Protein family/group databases

TCDBi2.A.1.1.30. the major facilitator superfamily (mfs).

Proteomic databases

MaxQBiP32467.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR092C; YHR092C; YHR092C.
GeneIDi856492.
KEGGisce:YHR092C.

Organism-specific databases

EuPathDBiFungiDB:YHR092C.
SGDiS000001134. HXT4.

Phylogenomic databases

GeneTreeiENSGT00720000108754.
HOGENOMiHOG000202870.
InParanoidiP32467.
KOiK08139.
OMAiEVNTLWE.
OrthoDBiEOG73JM4H.

Enzyme and pathway databases

BioCyciYEAST:G3O-31139-MONOMER.
ReactomeiR-SCE-428790. Facilitative Na+-independent glucose transporters.

Miscellaneous databases

NextBioi982200.
PROiP32467.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport_like.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "High-copy suppression of glucose transport defects by HXT4 and regulatory elements in the promoters of the HXT genes in Saccharomyces cerevisiae."
    Theodoris G., Fong N.M., Coons D.M., Bisson L.F.
    Genetics 137:957-966(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Low-affinity glucose carrier gene LGT1 of Saccharomyces cerevisiae, a homologue of the Kluyveromyces lactis RAG1 gene."
    Prior C., Fukuhara H., Blaisonneau J., Wesolowski-Louvel M.
    Yeast 9:1373-1377(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 547; 565 AND 567.
    Strain: ATCC 204508 / S288c.
  5. "A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
    Hitchcock A.L., Auld K., Gygi S.P., Silver P.A.
    Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-45.
  6. "Xylose transport studies with xylose-utilizing Saccharomyces cerevisiae strains expressing heterologous and homologous permeases."
    Saloheimo A., Rauta J., Stasyk O.V., Sibirny A.A., Penttila M., Ruohonen L.
    Appl. Microbiol. Biotechnol. 74:1041-1052(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, IDENTIFICATION OF FRAMESHIFT.

Entry informationi

Entry nameiHXT4_YEAST
AccessioniPrimary (citable) accession number: P32467
Secondary accession number(s): D3DL44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 11, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Glucose transport is thought to be mediated by two kinetically distinct systems, a glucose-repressible high-affinity system and a constitutive low-affinity system.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.