Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P32465

- HXT1_YEAST

UniProt

P32465 - HXT1_YEAST

Protein

Low-affinity glucose transporter HXT1

Gene

HXT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Low-affinity glucose transporter. HXT1 is as well involved in the transport of mannose.

    GO - Molecular functioni

    1. fructose transmembrane transporter activity Source: SGD
    2. galactose transmembrane transporter activity Source: SGD
    3. glucose transmembrane transporter activity Source: SGD
    4. mannose transmembrane transporter activity Source: SGD
    5. pentose transmembrane transporter activity Source: SGD

    GO - Biological processi

    1. fructose transport Source: GOC
    2. galactose transport Source: GOC
    3. glucose transport Source: SGD
    4. mannose transport Source: SGD
    5. pentose transport Source: GOC

    Keywords - Biological processi

    Sugar transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31140-MONOMER.

    Protein family/group databases

    TCDBi2.A.1.1.108. the major facilitator superfamily (mfs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Low-affinity glucose transporter HXT1
    Gene namesi
    Name:HXT1
    Synonyms:HOR4
    Ordered Locus Names:YHR094C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR094c.
    SGDiS000001136. HXT1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 570570Low-affinity glucose transporter HXT1PRO_0000050391Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei23 – 231Phosphoserine1 Publication
    Modified residuei38 – 381Phosphoserine2 Publications
    Modified residuei44 – 441Phosphoserine2 Publications
    Glycosylationi228 – 2281N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP32465.
    PaxDbiP32465.
    PeptideAtlasiP32465.

    Expressioni

    Developmental stagei

    Expression is maximal during lag and early exponential phases of growth, decreasing upon further entry into exponential growth.

    Inductioni

    Repressed at high glucose concentrations.

    Gene expression databases

    GenevestigatoriP32465.

    Interactioni

    Protein-protein interaction databases

    BioGridi36528. 100 interactions.
    DIPiDIP-5593N.
    IntActiP32465. 38 interactions.
    MINTiMINT-547595.
    STRINGi4932.YHR094C.

    Structurei

    3D structure databases

    ProteinModelPortaliP32465.
    SMRiP32465. Positions 70-528.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 6060CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini82 – 11635ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini138 – 1436CytoplasmicSequence Analysis
    Topological domaini165 – 17410ExtracellularSequence Analysis
    Topological domaini196 – 2016CytoplasmicSequence Analysis
    Topological domaini223 – 23614ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini258 – 34083CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini358 – 3636ExtracellularSequence Analysis
    Topological domaini382 – 3887CytoplasmicSequence Analysis
    Topological domaini410 – 43122ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini453 – 46917CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini491 – 4911ExtracellularSequence Analysis
    Topological domaini513 – 57058CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei61 – 8121Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei117 – 13721Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei144 – 16421Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei175 – 19521Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei202 – 22221Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei237 – 25721Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei341 – 35717Helical; Name=7Sequence AnalysisAdd
    BLAST
    Transmembranei364 – 38118Helical; Name=8Sequence AnalysisAdd
    BLAST
    Transmembranei389 – 40921Helical; Name=9Sequence AnalysisAdd
    BLAST
    Transmembranei432 – 45221Helical; Name=10Sequence AnalysisAdd
    BLAST
    Transmembranei470 – 49021Helical; Name=11Sequence AnalysisAdd
    BLAST
    Transmembranei492 – 51221Helical; Name=12Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0477.
    GeneTreeiENSGT00720000108754.
    HOGENOMiHOG000202870.
    KOiK08139.
    OMAiCMSIASA.
    OrthoDBiEOG73JM4H.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR005828. Sub_transporter.
    IPR003663. Sugar/inositol_transpt.
    IPR005829. Sugar_transporter_CS.
    [Graphical view]
    PfamiPF00083. Sugar_tr. 1 hit.
    [Graphical view]
    PRINTSiPR00171. SUGRTRNSPORT.
    SUPFAMiSSF103473. SSF103473. 2 hits.
    TIGRFAMsiTIGR00879. SP. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    PS00216. SUGAR_TRANSPORT_1. 1 hit.
    PS00217. SUGAR_TRANSPORT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32465-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNSTPDLISP QKSNSSNSYE LESGRSKAMN TPEGKNESFH DNLSESQVQP    50
    AVAPPNTGKG VYVTVSICCV MVAFGGFIFG WDTGTISGFV AQTDFLRRFG 100
    MKHHDGSHYL SKVRTGLIVS IFNIGCAIGG IVLAKLGDMY GRRIGLIVVV 150
    VIYTIGIIIQ IASINKWYQY FIGRIISGLG VGGITVLSPM LISEVAPSEM 200
    RGTLVSCYQV MITLGIFLGY CTNFGTKNYS NSVQWRVPLG LCFAWALFMI 250
    GGMMFVPESP RYLVEAGRID EARASLAKVN KCPPDHPYIQ YELETIEASV 300
    EEMRAAGTAS WGELFTGKPA MFQRTMMGIM IQSLQQLTGD NYFFYYGTIV 350
    FQAVGLSDSF ETSIVFGVVN FFSTCCSLYT VDRFGRRNCL MWGAVGMVCC 400
    YVVYASVGVT RLWPNGQDQP SSKGAGNCMI VFACFYIFCF ATTWAPIAYV 450
    VISECFPLRV KSKCMSIASA ANWIWGFLIS FFTPFITGAI NFYYGYVFMG 500
    CMVFAYFYVF FFVPETKGLS LEEVNDMYAE GVLPWKSASW VPVSKRGADY 550
    NADDLMHDDQ PFYKSLFSRK 570
    Length:570
    Mass (Da):63,261
    Last modified:October 1, 1993 - v1
    Checksum:i311CBB35AE80D19E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti70 – 701Missing in AAA34700. (PubMed:2046678)Curated
    Sequence conflicti469 – 4691S → T in AAA34700. (PubMed:2046678)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07079 Genomic DNA. Translation: AAB59311.1.
    M82963 Genomic DNA. Translation: AAA34700.1.
    U00060 Genomic DNA. Translation: AAB68933.1.
    BK006934 Genomic DNA. Translation: DAA06789.1.
    PIRiS38798.
    RefSeqiNP_011962.1. NM_001179224.1.

    Genome annotation databases

    EnsemblFungiiYHR094C; YHR094C; YHR094C.
    GeneIDi856494.
    KEGGisce:YHR094C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07079 Genomic DNA. Translation: AAB59311.1 .
    M82963 Genomic DNA. Translation: AAA34700.1 .
    U00060 Genomic DNA. Translation: AAB68933.1 .
    BK006934 Genomic DNA. Translation: DAA06789.1 .
    PIRi S38798.
    RefSeqi NP_011962.1. NM_001179224.1.

    3D structure databases

    ProteinModelPortali P32465.
    SMRi P32465. Positions 70-528.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36528. 100 interactions.
    DIPi DIP-5593N.
    IntActi P32465. 38 interactions.
    MINTi MINT-547595.
    STRINGi 4932.YHR094C.

    Protein family/group databases

    TCDBi 2.A.1.1.108. the major facilitator superfamily (mfs).

    Proteomic databases

    MaxQBi P32465.
    PaxDbi P32465.
    PeptideAtlasi P32465.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR094C ; YHR094C ; YHR094C .
    GeneIDi 856494.
    KEGGi sce:YHR094C.

    Organism-specific databases

    CYGDi YHR094c.
    SGDi S000001136. HXT1.

    Phylogenomic databases

    eggNOGi COG0477.
    GeneTreei ENSGT00720000108754.
    HOGENOMi HOG000202870.
    KOi K08139.
    OMAi CMSIASA.
    OrthoDBi EOG73JM4H.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31140-MONOMER.

    Miscellaneous databases

    NextBioi 982203.

    Gene expression databases

    Genevestigatori P32465.

    Family and domain databases

    InterProi IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR005828. Sub_transporter.
    IPR003663. Sugar/inositol_transpt.
    IPR005829. Sugar_transporter_CS.
    [Graphical view ]
    Pfami PF00083. Sugar_tr. 1 hit.
    [Graphical view ]
    PRINTSi PR00171. SUGRTRNSPORT.
    SUPFAMi SSF103473. SSF103473. 2 hits.
    TIGRFAMsi TIGR00879. SP. 1 hit.
    PROSITEi PS50850. MFS. 1 hit.
    PS00216. SUGAR_TRANSPORT_1. 1 hit.
    PS00217. SUGAR_TRANSPORT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The HXT1 gene product of Saccharomyces cerevisiae is a new member of the family of hexose transporters."
      Lewis D.A., Bisson L.F.
      Mol. Cell. Biol. 11:3804-3813(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Roles of multiple glucose transporters in Saccharomyces cerevisiae."
      Ko C.H., Liang H., Gaber R.F.
      Mol. Cell. Biol. 13:638-648(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-38 AND SER-44, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-38 AND SER-44, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiHXT1_YEAST
    AccessioniPrimary (citable) accession number: P32465
    Secondary accession number(s): D3DL45
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 135 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Glucose transport is thought to be mediated by two kinetically distinct systems, a glucose-repressible high-affinity system and a constitutive low-affinity system.
    Present with 23300 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3