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Protein

Low-affinity glucose transporter HXT1

Gene

HXT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Low-affinity glucose transporter. HXT1 is as well involved in the transport of mannose.

GO - Molecular functioni

  • fructose transmembrane transporter activity Source: SGD
  • galactose transmembrane transporter activity Source: SGD
  • glucose transmembrane transporter activity Source: SGD
  • mannose transmembrane transporter activity Source: SGD
  • pentose transmembrane transporter activity Source: SGD
  • sugar:proton symporter activity Source: GO_Central

GO - Biological processi

  • glucose import Source: GO_Central
  • glucose transport Source: SGD
  • mannose transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Sugar transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31140-MONOMER.

Protein family/group databases

TCDBi2.A.1.1.108. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Low-affinity glucose transporter HXT1
Gene namesi
Name:HXT1
Synonyms:HOR4
Ordered Locus Names:YHR094C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR094C.
SGDiS000001136. HXT1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 60CytoplasmicSequence analysisAdd BLAST60
Transmembranei61 – 81Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini82 – 116ExtracellularSequence analysisAdd BLAST35
Transmembranei117 – 137Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini138 – 143CytoplasmicSequence analysis6
Transmembranei144 – 164Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini165 – 174ExtracellularSequence analysis10
Transmembranei175 – 195Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini196 – 201CytoplasmicSequence analysis6
Transmembranei202 – 222Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini223 – 236ExtracellularSequence analysisAdd BLAST14
Transmembranei237 – 257Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini258 – 340CytoplasmicSequence analysisAdd BLAST83
Transmembranei341 – 357Helical; Name=7Sequence analysisAdd BLAST17
Topological domaini358 – 363ExtracellularSequence analysis6
Transmembranei364 – 381Helical; Name=8Sequence analysisAdd BLAST18
Topological domaini382 – 388CytoplasmicSequence analysis7
Transmembranei389 – 409Helical; Name=9Sequence analysisAdd BLAST21
Topological domaini410 – 431ExtracellularSequence analysisAdd BLAST22
Transmembranei432 – 452Helical; Name=10Sequence analysisAdd BLAST21
Topological domaini453 – 469CytoplasmicSequence analysisAdd BLAST17
Transmembranei470 – 490Helical; Name=11Sequence analysisAdd BLAST21
Topological domaini491ExtracellularSequence analysis1
Transmembranei492 – 512Helical; Name=12Sequence analysisAdd BLAST21
Topological domaini513 – 570CytoplasmicSequence analysisAdd BLAST58

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000503911 – 570Low-affinity glucose transporter HXT1Add BLAST570

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9PhosphoserineBy similarity1
Modified residuei13PhosphoserineBy similarity1
Modified residuei23PhosphoserineCombined sources1
Modified residuei26PhosphoserineBy similarity1
Modified residuei38PhosphoserineCombined sources1
Modified residuei44PhosphoserineCombined sources1
Modified residuei46PhosphoserineBy similarity1
Glycosylationi228N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP32465.
PRIDEiP32465.

PTM databases

iPTMnetiP32465.

Expressioni

Developmental stagei

Expression is maximal during lag and early exponential phases of growth, decreasing upon further entry into exponential growth.

Inductioni

Repressed at high glucose concentrations.

Interactioni

Protein-protein interaction databases

BioGridi36528. 103 interactors.
DIPiDIP-5593N.
IntActiP32465. 38 interactors.
MINTiMINT-547595.

Structurei

3D structure databases

ProteinModelPortaliP32465.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00850000132516.
HOGENOMiHOG000202870.
InParanoidiP32465.
KOiK08139.
OrthoDBiEOG092C1Q5W.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport-like.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32465-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSTPDLISP QKSNSSNSYE LESGRSKAMN TPEGKNESFH DNLSESQVQP
60 70 80 90 100
AVAPPNTGKG VYVTVSICCV MVAFGGFIFG WDTGTISGFV AQTDFLRRFG
110 120 130 140 150
MKHHDGSHYL SKVRTGLIVS IFNIGCAIGG IVLAKLGDMY GRRIGLIVVV
160 170 180 190 200
VIYTIGIIIQ IASINKWYQY FIGRIISGLG VGGITVLSPM LISEVAPSEM
210 220 230 240 250
RGTLVSCYQV MITLGIFLGY CTNFGTKNYS NSVQWRVPLG LCFAWALFMI
260 270 280 290 300
GGMMFVPESP RYLVEAGRID EARASLAKVN KCPPDHPYIQ YELETIEASV
310 320 330 340 350
EEMRAAGTAS WGELFTGKPA MFQRTMMGIM IQSLQQLTGD NYFFYYGTIV
360 370 380 390 400
FQAVGLSDSF ETSIVFGVVN FFSTCCSLYT VDRFGRRNCL MWGAVGMVCC
410 420 430 440 450
YVVYASVGVT RLWPNGQDQP SSKGAGNCMI VFACFYIFCF ATTWAPIAYV
460 470 480 490 500
VISECFPLRV KSKCMSIASA ANWIWGFLIS FFTPFITGAI NFYYGYVFMG
510 520 530 540 550
CMVFAYFYVF FFVPETKGLS LEEVNDMYAE GVLPWKSASW VPVSKRGADY
560 570
NADDLMHDDQ PFYKSLFSRK
Length:570
Mass (Da):63,261
Last modified:October 1, 1993 - v1
Checksum:i311CBB35AE80D19E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti70Missing in AAA34700 (PubMed:2046678).Curated1
Sequence conflicti469S → T in AAA34700 (PubMed:2046678).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07079 Genomic DNA. Translation: AAB59311.1.
M82963 Genomic DNA. Translation: AAA34700.1.
U00060 Genomic DNA. Translation: AAB68933.1.
BK006934 Genomic DNA. Translation: DAA06789.1.
PIRiS38798.
RefSeqiNP_011962.1. NM_001179224.1.

Genome annotation databases

EnsemblFungiiYHR094C; YHR094C; YHR094C.
GeneIDi856494.
KEGGisce:YHR094C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07079 Genomic DNA. Translation: AAB59311.1.
M82963 Genomic DNA. Translation: AAA34700.1.
U00060 Genomic DNA. Translation: AAB68933.1.
BK006934 Genomic DNA. Translation: DAA06789.1.
PIRiS38798.
RefSeqiNP_011962.1. NM_001179224.1.

3D structure databases

ProteinModelPortaliP32465.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36528. 103 interactors.
DIPiDIP-5593N.
IntActiP32465. 38 interactors.
MINTiMINT-547595.

Protein family/group databases

TCDBi2.A.1.1.108. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiP32465.

Proteomic databases

MaxQBiP32465.
PRIDEiP32465.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR094C; YHR094C; YHR094C.
GeneIDi856494.
KEGGisce:YHR094C.

Organism-specific databases

EuPathDBiFungiDB:YHR094C.
SGDiS000001136. HXT1.

Phylogenomic databases

GeneTreeiENSGT00850000132516.
HOGENOMiHOG000202870.
InParanoidiP32465.
KOiK08139.
OrthoDBiEOG092C1Q5W.

Enzyme and pathway databases

BioCyciYEAST:G3O-31140-MONOMER.

Miscellaneous databases

PROiP32465.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport-like.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHXT1_YEAST
AccessioniPrimary (citable) accession number: P32465
Secondary accession number(s): D3DL45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 30, 2016
This is version 156 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Glucose transport is thought to be mediated by two kinetically distinct systems, a glucose-repressible high-affinity system and a constitutive low-affinity system.
Present with 23300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.