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P32465

- HXT1_YEAST

UniProt

P32465 - HXT1_YEAST

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Protein

Low-affinity glucose transporter HXT1

Gene
HXT1, HOR4, YHR094C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Low-affinity glucose transporter. HXT1 is as well involved in the transport of mannose.

GO - Molecular functioni

  1. fructose transmembrane transporter activity Source: SGD
  2. galactose transmembrane transporter activity Source: SGD
  3. glucose transmembrane transporter activity Source: SGD
  4. mannose transmembrane transporter activity Source: SGD
  5. pentose transmembrane transporter activity Source: SGD

GO - Biological processi

  1. fructose transport Source: GOC
  2. galactose transport Source: GOC
  3. glucose transport Source: SGD
  4. mannose transport Source: SGD
  5. pentose transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Sugar transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31140-MONOMER.

Protein family/group databases

TCDBi2.A.1.1.108. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Low-affinity glucose transporter HXT1
Gene namesi
Name:HXT1
Synonyms:HOR4
Ordered Locus Names:YHR094C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR094c.
SGDiS000001136. HXT1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6060Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei61 – 8121Helical; Name=1; Reviewed predictionAdd
BLAST
Topological domaini82 – 11635Extracellular Reviewed predictionAdd
BLAST
Transmembranei117 – 13721Helical; Name=2; Reviewed predictionAdd
BLAST
Topological domaini138 – 1436Cytoplasmic Reviewed prediction
Transmembranei144 – 16421Helical; Name=3; Reviewed predictionAdd
BLAST
Topological domaini165 – 17410Extracellular Reviewed prediction
Transmembranei175 – 19521Helical; Name=4; Reviewed predictionAdd
BLAST
Topological domaini196 – 2016Cytoplasmic Reviewed prediction
Transmembranei202 – 22221Helical; Name=5; Reviewed predictionAdd
BLAST
Topological domaini223 – 23614Extracellular Reviewed predictionAdd
BLAST
Transmembranei237 – 25721Helical; Name=6; Reviewed predictionAdd
BLAST
Topological domaini258 – 34083Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei341 – 35717Helical; Name=7; Reviewed predictionAdd
BLAST
Topological domaini358 – 3636Extracellular Reviewed prediction
Transmembranei364 – 38118Helical; Name=8; Reviewed predictionAdd
BLAST
Topological domaini382 – 3887Cytoplasmic Reviewed prediction
Transmembranei389 – 40921Helical; Name=9; Reviewed predictionAdd
BLAST
Topological domaini410 – 43122Extracellular Reviewed predictionAdd
BLAST
Transmembranei432 – 45221Helical; Name=10; Reviewed predictionAdd
BLAST
Topological domaini453 – 46917Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei470 – 49021Helical; Name=11; Reviewed predictionAdd
BLAST
Topological domaini491 – 4911Extracellular Reviewed prediction
Transmembranei492 – 51221Helical; Name=12; Reviewed predictionAdd
BLAST
Topological domaini513 – 57058Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 570570Low-affinity glucose transporter HXT1PRO_0000050391Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei23 – 231Phosphoserine1 Publication
Modified residuei38 – 381Phosphoserine2 Publications
Modified residuei44 – 441Phosphoserine2 Publications
Glycosylationi228 – 2281N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP32465.
PaxDbiP32465.
PeptideAtlasiP32465.

Expressioni

Developmental stagei

Expression is maximal during lag and early exponential phases of growth, decreasing upon further entry into exponential growth.

Inductioni

Repressed at high glucose concentrations.

Gene expression databases

GenevestigatoriP32465.

Interactioni

Protein-protein interaction databases

BioGridi36528. 100 interactions.
DIPiDIP-5593N.
IntActiP32465. 38 interactions.
MINTiMINT-547595.
STRINGi4932.YHR094C.

Structurei

3D structure databases

ProteinModelPortaliP32465.
SMRiP32465. Positions 70-528.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0477.
GeneTreeiENSGT00720000108754.
HOGENOMiHOG000202870.
KOiK08139.
OMAiCMSIASA.
OrthoDBiEOG73JM4H.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR005828. Sub_transporter.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32465-1 [UniParc]FASTAAdd to Basket

« Hide

MNSTPDLISP QKSNSSNSYE LESGRSKAMN TPEGKNESFH DNLSESQVQP    50
AVAPPNTGKG VYVTVSICCV MVAFGGFIFG WDTGTISGFV AQTDFLRRFG 100
MKHHDGSHYL SKVRTGLIVS IFNIGCAIGG IVLAKLGDMY GRRIGLIVVV 150
VIYTIGIIIQ IASINKWYQY FIGRIISGLG VGGITVLSPM LISEVAPSEM 200
RGTLVSCYQV MITLGIFLGY CTNFGTKNYS NSVQWRVPLG LCFAWALFMI 250
GGMMFVPESP RYLVEAGRID EARASLAKVN KCPPDHPYIQ YELETIEASV 300
EEMRAAGTAS WGELFTGKPA MFQRTMMGIM IQSLQQLTGD NYFFYYGTIV 350
FQAVGLSDSF ETSIVFGVVN FFSTCCSLYT VDRFGRRNCL MWGAVGMVCC 400
YVVYASVGVT RLWPNGQDQP SSKGAGNCMI VFACFYIFCF ATTWAPIAYV 450
VISECFPLRV KSKCMSIASA ANWIWGFLIS FFTPFITGAI NFYYGYVFMG 500
CMVFAYFYVF FFVPETKGLS LEEVNDMYAE GVLPWKSASW VPVSKRGADY 550
NADDLMHDDQ PFYKSLFSRK 570
Length:570
Mass (Da):63,261
Last modified:October 1, 1993 - v1
Checksum:i311CBB35AE80D19E
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti70 – 701Missing in AAA34700. 1 Publication
Sequence conflicti469 – 4691S → T in AAA34700. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L07079 Genomic DNA. Translation: AAB59311.1.
M82963 Genomic DNA. Translation: AAA34700.1.
U00060 Genomic DNA. Translation: AAB68933.1.
BK006934 Genomic DNA. Translation: DAA06789.1.
PIRiS38798.
RefSeqiNP_011962.1. NM_001179224.1.

Genome annotation databases

EnsemblFungiiYHR094C; YHR094C; YHR094C.
GeneIDi856494.
KEGGisce:YHR094C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L07079 Genomic DNA. Translation: AAB59311.1 .
M82963 Genomic DNA. Translation: AAA34700.1 .
U00060 Genomic DNA. Translation: AAB68933.1 .
BK006934 Genomic DNA. Translation: DAA06789.1 .
PIRi S38798.
RefSeqi NP_011962.1. NM_001179224.1.

3D structure databases

ProteinModelPortali P32465.
SMRi P32465. Positions 70-528.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36528. 100 interactions.
DIPi DIP-5593N.
IntActi P32465. 38 interactions.
MINTi MINT-547595.
STRINGi 4932.YHR094C.

Protein family/group databases

TCDBi 2.A.1.1.108. the major facilitator superfamily (mfs).

Proteomic databases

MaxQBi P32465.
PaxDbi P32465.
PeptideAtlasi P32465.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR094C ; YHR094C ; YHR094C .
GeneIDi 856494.
KEGGi sce:YHR094C.

Organism-specific databases

CYGDi YHR094c.
SGDi S000001136. HXT1.

Phylogenomic databases

eggNOGi COG0477.
GeneTreei ENSGT00720000108754.
HOGENOMi HOG000202870.
KOi K08139.
OMAi CMSIASA.
OrthoDBi EOG73JM4H.

Enzyme and pathway databases

BioCyci YEAST:G3O-31140-MONOMER.

Miscellaneous databases

NextBioi 982203.

Gene expression databases

Genevestigatori P32465.

Family and domain databases

InterProi IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR005828. Sub_transporter.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view ]
Pfami PF00083. Sugar_tr. 1 hit.
[Graphical view ]
PRINTSi PR00171. SUGRTRNSPORT.
SUPFAMi SSF103473. SSF103473. 2 hits.
TIGRFAMsi TIGR00879. SP. 1 hit.
PROSITEi PS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The HXT1 gene product of Saccharomyces cerevisiae is a new member of the family of hexose transporters."
    Lewis D.A., Bisson L.F.
    Mol. Cell. Biol. 11:3804-3813(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Roles of multiple glucose transporters in Saccharomyces cerevisiae."
    Ko C.H., Liang H., Gaber R.F.
    Mol. Cell. Biol. 13:638-648(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-38 AND SER-44, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-38 AND SER-44, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHXT1_YEAST
AccessioniPrimary (citable) accession number: P32465
Secondary accession number(s): D3DL45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 14, 2014
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Glucose transport is thought to be mediated by two kinetically distinct systems, a glucose-repressible high-affinity system and a constitutive low-affinity system.
Present with 23300 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

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