Low-affinity glucose transporter HXT1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P32465 (HXT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 124. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Low-affinity glucose transporter HXT1

Gene names

Name:	HXT1
Synonyms:	HOR4
Ordered Locus Names:	YHR094C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Low-affinity glucose transporter. HXT1 is as well involved in the transport of mannose.
Subcellular location	Membrane; Multi-pass membrane protein.
Developmental stage	Expression is maximal during lag and early exponential phases of growth, decreasing upon further entry into exponential growth.
Induction	Repressed at high glucose concentrations.
Miscellaneous	Glucose transport is thought to be mediated by two kinetically distinct systems, a glucose-repressible high-affinity system and a constitutive low-affinity system. Present with 23300 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. [View classification]

Ontologies

Keywords
Biological process	Sugar transport Transport
Cellular component	Membrane
Domain	Repeat Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD plasma membrane Inferred from direct assay PubMed 10377429. Source: SGD
Molecular_function	fructose transmembrane transporter activity Inferred from mutant phenotype PubMed 7651133. Source: SGD galactose transmembrane transporter activity Traceable author statement PubMed 10618490. Source: SGD glucose transmembrane transporter activity Traceable author statement PubMed 10618490. Source: SGD mannose transmembrane transporter activity Traceable author statement PubMed 10618490. Source: SGD pentose transmembrane transporter activity Inferred from mutant phenotype PubMed 17180689. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 570

570

Low-affinity glucose transporter HXT1

PRO_0000050391

Regions

Topological domain

1 – 60

Cytoplasmic Potential

Transmembrane

61 – 81

Helical; Name=1; Potential

Topological domain

82 – 116

Extracellular Potential

Transmembrane

117 – 137

Helical; Name=2; Potential

Topological domain

138 – 143

Cytoplasmic Potential

Transmembrane

144 – 164

Helical; Name=3; Potential

Topological domain

165 – 174

Extracellular Potential

Transmembrane

175 – 195

Helical; Name=4; Potential

Topological domain

196 – 201

Cytoplasmic Potential

Transmembrane

202 – 222

Helical; Name=5; Potential

Topological domain

223 – 236

Extracellular Potential

Transmembrane

237 – 257

Helical; Name=6; Potential

Topological domain

258 – 340

Cytoplasmic Potential

Transmembrane

341 – 357

Helical; Name=7; Potential

Topological domain

358 – 363

Extracellular Potential

Transmembrane

364 – 381

Helical; Name=8; Potential

Topological domain

382 – 388

Cytoplasmic Potential

Transmembrane

389 – 409

Helical; Name=9; Potential

Topological domain

410 – 431

Extracellular Potential

Transmembrane

432 – 452

Helical; Name=10; Potential

Topological domain

453 – 469

Cytoplasmic Potential

Transmembrane

470 – 490

Helical; Name=11; Potential

Topological domain

491

Extracellular Potential

Transmembrane

492 – 512

Helical; Name=12; Potential

Topological domain

513 – 570

Cytoplasmic Potential

Amino acid modifications

Modified residue

Phosphoserine Ref.9 Ref.10

Modified residue

Phosphoserine Ref.10

Modified residue

Phosphoserine Ref.9 Ref.10

Modified residue

Phosphoserine Ref.8 Ref.10

Modified residue

Phosphoserine Ref.8 Ref.10

Modified residue

Phosphoserine Ref.10

Modified residue

528

Phosphotyrosine Ref.6

Glycosylation

228

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

Missing in AAA34700. Ref.1

Sequence conflict

469

S → T in AAA34700. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P32465 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 311CBB35AE80D19E
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FASTA

570

63,261

        10         20         30         40         50         60 
MNSTPDLISP QKSNSSNSYE LESGRSKAMN TPEGKNESFH DNLSESQVQP AVAPPNTGKG 

        70         80         90        100        110        120 
VYVTVSICCV MVAFGGFIFG WDTGTISGFV AQTDFLRRFG MKHHDGSHYL SKVRTGLIVS 

       130        140        150        160        170        180 
IFNIGCAIGG IVLAKLGDMY GRRIGLIVVV VIYTIGIIIQ IASINKWYQY FIGRIISGLG 

       190        200        210        220        230        240 
VGGITVLSPM LISEVAPSEM RGTLVSCYQV MITLGIFLGY CTNFGTKNYS NSVQWRVPLG 

       250        260        270        280        290        300 
LCFAWALFMI GGMMFVPESP RYLVEAGRID EARASLAKVN KCPPDHPYIQ YELETIEASV 

       310        320        330        340        350        360 
EEMRAAGTAS WGELFTGKPA MFQRTMMGIM IQSLQQLTGD NYFFYYGTIV FQAVGLSDSF 

       370        380        390        400        410        420 
ETSIVFGVVN FFSTCCSLYT VDRFGRRNCL MWGAVGMVCC YVVYASVGVT RLWPNGQDQP 

       430        440        450        460        470        480 
SSKGAGNCMI VFACFYIFCF ATTWAPIAYV VISECFPLRV KSKCMSIASA ANWIWGFLIS 

       490        500        510        520        530        540 
FFTPFITGAI NFYYGYVFMG CMVFAYFYVF FFVPETKGLS LEEVNDMYAE GVLPWKSASW 

       550        560        570 
VPVSKRGADY NADDLMHDDQ PFYKSLFSRK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The HXT1 gene product of Saccharomyces cerevisiae is a new member of the family of hexose transporters." Lewis D.A., Bisson L.F. Mol. Cell. Biol. 11:3804-3813(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Roles of multiple glucose transporters in Saccharomyces cerevisiae." Ko C.H., Liang H., Gaber R.F. Mol. Cell. Biol. 13:638-648(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-528, MASS SPECTROMETRY. Strain: SUB592.
[7]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-38 AND SER-44, MASS SPECTROMETRY. Strain: ADR376.
[9]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16 AND SER-23, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16; SER-18; SER-23; SER-38; SER-44 AND SER-46, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L07079 Genomic DNA. Translation: AAB59311.1. M82963 Genomic DNA. Translation: AAA34700.1. U00060 Genomic DNA. Translation: AAB68933.1. BK006934 Genomic DNA. Translation: DAA06789.1.
PIR	S38798.
RefSeq	NP_011962.1. NM_001179224.1.
3D structure databases
ProteinModelPortal	P32465.
SMR	P32465. Positions 70-528.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5593N.
IntAct	P32465. 38 interactions.
MINT	MINT-547595.
STRING	4932.YHR094C.
Proteomic databases
PaxDb	P32465.
PeptideAtlas	P32465.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR094C; YHR094C; YHR094C.
GeneID	856494.
KEGG	sce:YHR094C.
Organism-specific databases
CYGD	YHR094c.
SGD	S000001136. HXT1.
Phylogenomic databases
eggNOG	COG0477.
GeneTree	ENSGT00650000093295.
HOGENOM	HOG000202870.
KO	K08139.
OrthoDB	EOG4SR153.
Enzyme and pathway databases
BioCyc	YEAST:G3O-31140-MONOMER.
Gene expression databases
ArrayExpress	P32465.
Genevestigator	P32465.
GermOnline	YHR094C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. IPR005828. Sub_transporter. IPR003663. Sugar/inositol_transpt. IPR005829. Sugar_transporter_CS. [Graphical view]
Pfam	PF00083. Sugar_tr. 1 hit. [Graphical view]
PRINTS	PR00171. SUGRTRNSPORT.
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMs	TIGR00879. SP. 1 hit.
PROSITE	PS50850. MFS. 1 hit. PS00216. SUGAR_TRANSPORT_1. 1 hit. PS00217. SUGAR_TRANSPORT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982203.

Entry information

Entry name

HXT1_YEAST

Accession

Primary (citable) accession number: P32465
Secondary accession number(s): D3DL45

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 29, 2013
This is version 124 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents