Reviewed, UniProtKB/Swiss-Prot P32461 (DPH2_YEAST)

Names and origin

Protein names

Recommended name:
    Diphthamide biosynthesis protein 2
Alternative name(s):
    Diphtheria toxin resistance protein 2

Gene names

Name:	DPH2
Ordered Locus Names:	YKL191W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	534 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for the first step of diphthamide biosynthesis, the transfer of 3-amino-3-carboxypropyl from S-adenosyl-L-methionine to a histidine residue. Diphthamide is a post-translational modification of histidine which occurs in elongation factor 2. Ref.1
Subunit structure	Interacts with the diphthamide biosynthesis proteins DPH1 and KTI11/DPH3. Ref.6 Ref.9
Subcellular location	Cytoplasm Ref.7.
Miscellaneous	Present with 3486 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the DPH1/DPH2 family. DPH2 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-diphthamide biosynthetic process from peptidyl-histidine Ref.1 Ref.9 Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Ref.7 Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DPH1	P40487	1	EBI-6090,EBI-25162
EFT1	P32324	1	EBI-6090,EBI-6333

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 534	534	Diphthamide biosynthesis protein 2		PRO_0000083394
Amino acid modifications
	Modified residue	496	1	Phosphoserine Ref.10

Sequences

	Sequence		Length	Mass (Da)
	P32461-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 9F2728DAE0A27431	FASTA	534	59,775
	10 20 30 40 50 60 MEVAPALSTT QSDVAFQKVE THEIDRSSYL GPCYNSDELM QLISAYYNVE PLVGYLEQHP 70 80 90 100 110 120 EYQNVTLQFP DDLIKDSSLI VRLLQSKFPH GKIKFWVLAD TAYSACCVDE VAAEHVHAEV 130 140 150 160 170 180 VVHFGDACLN AIQNLPVVYS FGTPFLDLAL VVENFQRAFP DLSSKICLMA NAPFSKHLSQ 190 200 210 220 230 240 LYNILKGDLH YTNIIYSQVN TSAVEEKFVT ILDTFHVPED VDQVGVFEKN SVLFGQHDKA 250 260 270 280 290 300 DNISPEDYHL FHLTTPQDPR LLYLSTVFQS VHIFDPALPG MVTGPFPSLM RRYKYMHVAR 310 320 330 340 350 360 TAGCIGILVN TLSLRNTRET INELVKLIKT REKKHYLFVV GKPNVAKLAN FEDIDIWCIL 370 380 390 400 410 420 GCSQSGIIVD QFNEFYKPII TPYELNLALS EEVTWTGKWV VDFRDAIDEI EQNLGGQDTI 430 440 450 460 470 480 SASTTSDEPE FDVVRGRYTS TSRPLRALTH LELEAADDDD SKQLTTRHTA SGAVIKGTVS 490 500 510 520 530 TSASALQNRS WKGLGSDFDS TEVDNTGADI EEGISGVARG YGFDREDAMK KENK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Diphthamide synthesis in Saccharomyces cerevisiae: structure of the DPH2 gene." Mattheakis L.C., Sor F., Collier R.J. Gene 132:149-154(1993) [PubMed: 8406038] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: S288c / GRF88.
[2]	"DNA sequence analysis of the YCN2 region of chromosome XI in Saccharomyces cerevisiae." Cheret G., Mattheakis L.C., Sor F. Yeast 9:661-667(1993) [PubMed: 8394042] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / GRF88.
[3]	"Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene." Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J., Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W. Yeast 9:1343-1348(1993) [PubMed: 8154185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	"The protein phosphatase calcineurin is essential for NaCl tolerance of Saccharomyces cerevisiae." Mendoza I., Rubio F., Rodriguez-Navarro A., Pardo J.M. J. Biol. Chem. 269:8792-8796(1994) [PubMed: 8132612] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 258-534.
[6]	"Elongator's toxin-target (TOT) function is nuclear localization sequence dependent and suppressed by post-translational modification." Fichtner L., Jablonowski D., Schierhorn A., Kitamoto H.K., Stark M.J.R., Schaffrath R. Mol. Microbiol. 49:1297-1307(2003) [PubMed: 12940988] [Abstract] Cited for: INTERACTION WITH KTI11.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2." Liu S., Milne G.T., Kuremsky J.G., Fink G.R., Leppla S.H. Mol. Cell. Biol. 24:9487-9497(2004) [PubMed: 15485916] [Abstract] Cited for: INTERACTION WITH DPH1.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-496, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L01424 Genomic DNA. Translation: AAA64990.1. X69765 Genomic DNA. Translation: CAA49420.1. X74151 Genomic DNA. Translation: CAA52247.1. Z28191 Genomic DNA. Translation: CAA82035.1. Z26521 Genomic DNA. Translation: CAA81289.1.
PIR	S34679. B59228.
RefSeq	NP_012730.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5654N.
IntAct	P32461. 4 interactions.
STRING	P32461.
Genome annotation databases
Ensembl	YKL191W; YKL191W; YKL191W; Saccharomyces cerevisiae. [Genome view]
GeneID	853643.
KEGG	sce:YKL191W.
NMPDR	fig|4932.3.peg.3707.
Organism-specific databases
CYGD	YKL191w.
SGD	S000001674. DPH2.
Phylogenomic databases
HOGENOM	HBG446814.
OMA	CLMANAP.
OrthoDB	EOG9RBS1Z.
Gene expression databases
ArrayExpress	P32461.
Genevestigator	P32461.
GermOnline	YKL191W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002728. Diphthamide_synth_DHP1/DHP2. IPR010014. Diphthamide_synth_DHP2. [Graphical view]
PANTHER	PTHR10762. Diphthamide_syn. 1 hit. PTHR10762:SF2. DPH2. 1 hit.
Pfam	PF01866. Diphthamide_syn. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	974540.

Entry information

Entry name

DPH2_YEAST

Accession

Primary (citable) accession number: P32461

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	December 15, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)