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Protein

Ureidoglycolate lyase

Gene

DAL3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the catabolism of the allantoin degradation intermediate (S)-ureidoglycolate, generating urea and glyoxylate. Involved in the utilization of allantoin as secondary nitrogen source when primary sources are limiting.1 Publication

Catalytic activityi

(S)-ureidoglycolate = glyoxylate + urea.1 Publication

pH dependencei

Optimum pH is 7.3.1 Publication

Pathwayi

GO - Molecular functioni

  1. ureidoglycolate hydrolase activity Source: InterPro
  2. ureidoglycolate lyase activity Source: SGD

GO - Biological processi

  1. allantoin catabolic process Source: SGD
  2. purine nucleobase metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Purine metabolism

Enzyme and pathway databases

BioCyciMetaCyc:YIR032C-MONOMER.
YEAST:YIR032C-MONOMER.
UniPathwayiUPA00395.

Names & Taxonomyi

Protein namesi
Recommended name:
Ureidoglycolate lyase (EC:4.3.2.3)
Alternative name(s):
Degradation of allantoin protein 3
Ureidoglycolatase
Ureidoglycolate hydrolase
Gene namesi
Name:DAL3
Ordered Locus Names:YIR032C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IX

Organism-specific databases

CYGDiYIR032c.
EuPathDBiFungiDB:YIR032C.
SGDiS000001471. DAL3.

Subcellular locationi

GO - Cellular componenti

  1. membrane Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 195195Ureidoglycolate lyasePRO_0000120562Add
BLAST

Proteomic databases

MaxQBiP32459.
PaxDbiP32459.

Expressioni

Inductioni

Subject to nitrogen catabolite repression.1 Publication

Gene expression databases

GenevestigatoriP32459.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi35023. 17 interactions.
DIPiDIP-1284N.
IntActiP32459. 2 interactions.
MINTiMINT-393452.
STRINGi4932.YIR032C.

Structurei

3D structure databases

ProteinModelPortaliP32459.
SMRiP32459. Positions 1-194.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ureidoglycolate lyase family.Curated

Phylogenomic databases

eggNOGiCOG3194.
HOGENOMiHOG000256169.
InParanoidiP32459.
KOiK01483.
OMAiNWNIFRC.
OrthoDBiEOG7CG79G.

Family and domain databases

Gene3Di2.60.120.480. 1 hit.
InterProiIPR011051. RmlC_Cupin.
IPR007247. Ureidogly_lyase.
IPR024060. Ureidoglycolate_lyase_dom.
[Graphical view]
PANTHERiPTHR21221. PTHR21221. 1 hit.
PfamiPF04115. Ureidogly_hydro. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

P32459-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTVVAETLT KESFEEYGTI ISPDEEISRM QNLEKGANQG TAIKLLQVSQ
60 70 80 90 100
VENKSTSKVP NWNLFRCFPQ PHLNRVFTQG SNQAISHSIK VLEKHPCSTQ
110 120 130 140 150
TFVPMGRTSA EVAYLVVVAK EIGNKPDLST LRAFTCLGNQ AVTYGLGTWH
160 170 180 190
APMIVLGKEE HLDFSVLIYE SLDPDRPEKD CVEEHYSDGD VCIII
Length:195
Mass (Da):21,727
Last modified:October 1, 1993 - v1
Checksum:i6230AEE69585206B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64778 Genomic DNA. Translation: AAA73025.1.
Z38061 Genomic DNA. Translation: CAA86192.1.
BK006942 Genomic DNA. Translation: DAA08579.1.
PIRiS42022.
RefSeqiNP_012298.1. NM_001179554.1.

Genome annotation databases

EnsemblFungiiYIR032C; YIR032C; YIR032C.
GeneIDi854850.
KEGGisce:YIR032C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64778 Genomic DNA. Translation: AAA73025.1.
Z38061 Genomic DNA. Translation: CAA86192.1.
BK006942 Genomic DNA. Translation: DAA08579.1.
PIRiS42022.
RefSeqiNP_012298.1. NM_001179554.1.

3D structure databases

ProteinModelPortaliP32459.
SMRiP32459. Positions 1-194.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35023. 17 interactions.
DIPiDIP-1284N.
IntActiP32459. 2 interactions.
MINTiMINT-393452.
STRINGi4932.YIR032C.

Proteomic databases

MaxQBiP32459.
PaxDbiP32459.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIR032C; YIR032C; YIR032C.
GeneIDi854850.
KEGGisce:YIR032C.

Organism-specific databases

CYGDiYIR032c.
EuPathDBiFungiDB:YIR032C.
SGDiS000001471. DAL3.

Phylogenomic databases

eggNOGiCOG3194.
HOGENOMiHOG000256169.
InParanoidiP32459.
KOiK01483.
OMAiNWNIFRC.
OrthoDBiEOG7CG79G.

Enzyme and pathway databases

UniPathwayiUPA00395.
BioCyciMetaCyc:YIR032C-MONOMER.
YEAST:YIR032C-MONOMER.

Miscellaneous databases

NextBioi977749.
PROiP32459.

Gene expression databases

GenevestigatoriP32459.

Family and domain databases

Gene3Di2.60.120.480. 1 hit.
InterProiIPR011051. RmlC_Cupin.
IPR007247. Ureidogly_lyase.
IPR024060. Ureidoglycolate_lyase_dom.
[Graphical view]
PANTHERiPTHR21221. PTHR21221. 1 hit.
PfamiPF04115. Ureidogly_hydro. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The ureidoglycollate hydrolase (DAL3) gene in Saccharomyces cerevisiae."
    Yoo H.S., Cooper T.G.
    Yeast 7:693-698(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: BIOPHYSICOCHEMICAL PROPERTIES.
  5. "Identification of the ureidoglycolate hydrolase gene in the DAL gene cluster of Saccharomyces cerevisiae."
    Yoo H.S., Genbauffe F.S., Cooper T.G.
    Mol. Cell. Biol. 5:2279-2288(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, INDUCTION.

Entry informationi

Entry nameiALLA_YEAST
AccessioniPrimary (citable) accession number: P32459
Secondary accession number(s): D6VVW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: April 29, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

This enzyme was also named ureidoglycolate hydrolase in the literature. However, this is the recommended name of another enzyme (EC 3.5.3.19) acting on ureidoglycolate. To make the distinction between ammonia- or urea-releasing activities from ureidoglycolate, the use of this ambiguous name is deprecated.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.