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P32459 (ALLA_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ureidoglycolate lyase

EC=4.3.2.3
Alternative name(s):
Degradation of allantoin protein 3
Ureidoglycolatase
Ureidoglycolate hydrolase
Gene names
Name:DAL3
Ordered Locus Names:YIR032C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length195 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the catabolism of the allantoin degradation intermediate (S)-ureidoglycolate, generating urea and glyoxylate. Involved in the utilization of allantoin as secondary nitrogen source when primary sources are limiting. Ref.5

Catalytic activity

(S)-ureidoglycolate = glyoxylate + urea. Ref.5

Pathway

Nitrogen metabolism; (S)-allantoin degradation.

Subunit structure

Homodimer By similarity.

Induction

Subject to nitrogen catabolite repression. Ref.5

Sequence similarities

Belongs to the ureidoglycolate lyase family.

Caution

This enzyme was also named ureidoglycolate hydrolase in the literature. However, this is the recommended name of another enzyme (EC 3.5.3.19) acting on ureidoglycolate. To make the distinction between ammonia- or urea-releasing activities from ureidoglycolate, the use of this ambiguous name is deprecated.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.3. Ref.4

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 195195Ureidoglycolate lyase
PRO_0000120562

Sequences

Sequence LengthMass (Da)Tools
P32459 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 6230AEE69585206B

FASTA19521,727
        10         20         30         40         50         60 
MVTVVAETLT KESFEEYGTI ISPDEEISRM QNLEKGANQG TAIKLLQVSQ VENKSTSKVP 

        70         80         90        100        110        120 
NWNLFRCFPQ PHLNRVFTQG SNQAISHSIK VLEKHPCSTQ TFVPMGRTSA EVAYLVVVAK 

       130        140        150        160        170        180 
EIGNKPDLST LRAFTCLGNQ AVTYGLGTWH APMIVLGKEE HLDFSVLIYE SLDPDRPEKD 

       190 
CVEEHYSDGD VCIII 

« Hide

References

« Hide 'large scale' references
[1]"The ureidoglycollate hydrolase (DAL3) gene in Saccharomyces cerevisiae."
Yoo H.S., Cooper T.G.
Yeast 7:693-698(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The assay of yeast ureidoglycolatase."
Choi K.S., Lee K.W., Roush A.H.
Anal. Biochem. 17:413-422(1966) [PubMed] [Europe PMC] [Abstract]
Cited for: BIOPHYSICOCHEMICAL PROPERTIES.
[5]"Identification of the ureidoglycolate hydrolase gene in the DAL gene cluster of Saccharomyces cerevisiae."
Yoo H.S., Genbauffe F.S., Cooper T.G.
Mol. Cell. Biol. 5:2279-2288(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M64778 Genomic DNA. Translation: AAA73025.1.
Z38061 Genomic DNA. Translation: CAA86192.1.
BK006942 Genomic DNA. Translation: DAA08579.1.
PIRS42022.
RefSeqNP_012298.1. NM_001179554.1.

3D structure databases

ProteinModelPortalP32459.
SMRP32459. Positions 1-194.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35023. 17 interactions.
DIPDIP-1284N.
IntActP32459. 2 interactions.
MINTMINT-393452.
STRING4932.YIR032C.

Proteomic databases

PaxDbP32459.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIR032C; YIR032C; YIR032C.
GeneID854850.
KEGGsce:YIR032C.

Organism-specific databases

CYGDYIR032c.
SGDS000001471. DAL3.

Phylogenomic databases

eggNOGCOG3194.
HOGENOMHOG000256169.
KOK01483.
OMANWNIFRC.
OrthoDBEOG7CG79G.

Enzyme and pathway databases

BioCycMetaCyc:YIR032C-MONOMER.
YEAST:YIR032C-MONOMER.
UniPathwayUPA00395.

Gene expression databases

GenevestigatorP32459.

Family and domain databases

Gene3D2.60.120.480. 1 hit.
InterProIPR011051. RmlC_Cupin.
IPR007247. Ureidogly_hydro.
IPR024060. Ureidoglycolate_hydrolase_dom.
[Graphical view]
PANTHERPTHR21221. PTHR21221. 1 hit.
PfamPF04115. Ureidogly_hydro. 1 hit.
[Graphical view]
PIRSFPIRSF017306. Ureidogly_hydro. 1 hit.
SUPFAMSSF51182. SSF51182. 1 hit.
ProtoNetSearch...

Other

NextBio977749.

Entry information

Entry nameALLA_YEAST
AccessionPrimary (citable) accession number: P32459
Secondary accession number(s): D6VVW3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: February 19, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways