Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Guanylate-binding protein 1

Gene

GBP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes GTP to GMP in 2 consecutive cleavage reactions. Exhibits antiviral activity against influenza virus. Promote oxidative killing and deliver antimicrobial peptides to autophagolysosomes, providing broad host protection against different pathogen classes.1 Publication

Catalytic activityi

GTP + H2O = GDP + phosphate.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi45 – 528GTP
Nucleotide bindingi67 – 693GTP
Nucleotide bindingi97 – 1015GTP

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • cytokine binding Source: UniProtKB
  • enzyme binding Source: UniProtKB
  • GTPase activity Source: InterPro
  • GTP binding Source: ProtInc
  • Hsp90 protein binding Source: UniProtKB
  • identical protein binding Source: IntAct
  • spectrin binding Source: UniProtKB

GO - Biological processi

  • defense response to virus Source: UniProtKB-KW
  • interferon-gamma-mediated signaling pathway Source: Reactome
  • negative regulation of ERK1 and ERK2 cascade Source: UniProtKB
  • negative regulation of interleukin-2 secretion Source: UniProtKB
  • negative regulation of protein localization to plasma membrane Source: UniProtKB
  • negative regulation of substrate adhesion-dependent cell spreading Source: UniProtKB
  • negative regulation of T cell receptor signaling pathway Source: UniProtKB
  • regulation of calcium-mediated signaling Source: UniProtKB
  • regulation of protein localization to plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antiviral defense, Immunity

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-877300. Interferon gamma signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylate-binding protein 1 (EC:3.6.5.-2 Publications)
Alternative name(s):
GTP-binding protein 1
Short name:
GBP-1
Short name:
HuGBP-1
Guanine nucleotide-binding protein 1
Interferon-induced guanylate-binding protein 1
Gene namesi
Name:GBP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:4182. GBP1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • extracellular region Source: UniProtKB-SubCell
  • Golgi membrane Source: UniProtKB-SubCell
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi51 – 511K → A: Loss of GTPase activity. Constitutively monomeric. Expressed throughout the cytoplasm, loss of vesicular accumulation. 1 Publication
Mutagenesisi227 – 2282RK → EE: Constitutively dimeric. Localizes at vesicle-like structures at the plasma membrane. 1 Publication
Mutagenesisi589 – 5924Missing : Loss of association with membranes. 1 Publication

Organism-specific databases

PharmGKBiPA28596.

Polymorphism and mutation databases

BioMutaiGBP1.
DMDMi311033383.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 589589Guanylate-binding protein 1PRO_0000190963Add
BLAST
Propeptidei590 – 5923Removed in mature formPRO_0000396777

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei589 – 5891Cysteine methyl esterBy similarity
Lipidationi589 – 5891S-farnesyl cysteine1 Publication

Post-translational modificationi

Isoprenylation is required for proper subcellular location.1 Publication

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

EPDiP32455.
MaxQBiP32455.
PaxDbiP32455.
PeptideAtlasiP32455.
PRIDEiP32455.

PTM databases

iPTMnetiP32455.
PhosphoSiteiP32455.
SwissPalmiP32455.

Expressioni

Inductioni

By IFNG during macrophage activation, and by TNF and IL1B.1 Publication

Gene expression databases

BgeeiENSG00000117228.
CleanExiHS_GBP1.
GenevisibleiP32455. HS.

Organism-specific databases

HPAiCAB015450.

Interactioni

Subunit structurei

Homodimer; homodimerization occurs upon GTP-binding and is required for the second hydrolysis step from GDP to GMP. Heterodimer with other family members, including GBP2, GBP3, GBP4 and GBP5. Dimerization regulates subcellular location to membranous structures.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself7EBI-2869161,EBI-2869161

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • cytokine binding Source: UniProtKB
  • enzyme binding Source: UniProtKB
  • Hsp90 protein binding Source: UniProtKB
  • identical protein binding Source: IntAct
  • spectrin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi108903. 18 interactions.
DIPiDIP-60423N.
IntActiP32455. 1 interaction.
MINTiMINT-4718320.
STRINGi9606.ENSP00000359504.

Structurei

Secondary structure

1
592
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 177Combined sources
Beta strandi20 – 234Combined sources
Helixi25 – 328Combined sources
Beta strandi36 – 4611Combined sources
Helixi47 – 493Combined sources
Helixi51 – 588Combined sources
Beta strandi62 – 654Combined sources
Beta strandi70 – 723Combined sources
Beta strandi77 – 848Combined sources
Beta strandi86 – 883Combined sources
Beta strandi92 – 987Combined sources
Turni101 – 1033Combined sources
Helixi104 – 1063Combined sources
Helixi112 – 12211Combined sources
Beta strandi124 – 1329Combined sources
Helixi136 – 1405Combined sources
Helixi143 – 1464Combined sources
Helixi148 – 1514Combined sources
Beta strandi156 – 1583Combined sources
Helixi167 – 1704Combined sources
Helixi171 – 1744Combined sources
Beta strandi177 – 1837Combined sources
Beta strandi192 – 1943Combined sources
Helixi198 – 2058Combined sources
Helixi214 – 22916Combined sources
Beta strandi233 – 2375Combined sources
Helixi244 – 2529Combined sources
Helixi255 – 2573Combined sources
Helixi260 – 27617Combined sources
Turni283 – 2853Combined sources
Helixi290 – 30617Combined sources
Helixi312 – 34231Combined sources
Helixi350 – 37122Combined sources
Helixi376 – 3783Combined sources
Helixi379 – 42345Combined sources
Turni424 – 4274Combined sources
Helixi432 – 44918Combined sources
Helixi457 – 46711Combined sources
Helixi469 – 47810Combined sources
Beta strandi480 – 4823Combined sources
Helixi484 – 56380Combined sources
Helixi566 – 58217Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DG3X-ray1.80A1-592[»]
1F5NX-ray1.70A1-592[»]
2B8WX-ray2.22A/B1-317[»]
2B92X-ray3.20A/B1-317[»]
2BC9X-ray2.80A1-317[»]
2D4HX-ray2.90A/B1-317[»]
DisProtiDP00313.
ProteinModelPortaliP32455.
SMRiP32455. Positions 7-583.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32455.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 278244GB1/RHD3-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 311311GTPase domain (Globular)Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2037. Eukaryota.
ENOG410XR6Z. LUCA.
GeneTreeiENSGT00550000074475.
HOGENOMiHOG000266974.
HOVERGENiHBG001979.
InParanoidiP32455.
OMAiGFQKESR.
OrthoDBiEOG091G03P3.
PhylomeDBiP32455.
TreeFamiTF331602.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR030386. G_GB1_RHD3_dom.
IPR003191. Guanylate-bd_C.
IPR015894. Guanylate-bd_N.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF02263. GBP. 1 hit.
PF02841. GBP_C. 1 hit.
[Graphical view]
SUPFAMiSSF48340. SSF48340. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51715. G_GB1_RHD3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32455-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASEIHMTGP MCLIENTNGR LMANPEALKI LSAITQPMVV VAIVGLYRTG
60 70 80 90 100
KSYLMNKLAG KKKGFSLGST VQSHTKGIWM WCVPHPKKPG HILVLLDTEG
110 120 130 140 150
LGDVEKGDNQ NDSWIFALAV LLSSTFVYNS IGTINQQAMD QLYYVTELTH
160 170 180 190 200
RIRSKSSPDE NENEVEDSAD FVSFFPDFVW TLRDFSLDLE ADGQPLTPDE
210 220 230 240 250
YLTYSLKLKK GTSQKDETFN LPRLCIRKFF PKKKCFVFDR PVHRRKLAQL
260 270 280 290 300
EKLQDEELDP EFVQQVADFC SYIFSNSKTK TLSGGIQVNG PRLESLVLTY
310 320 330 340 350
VNAISSGDLP CMENAVLALA QIENSAAVQK AIAHYEQQMG QKVQLPTETL
360 370 380 390 400
QELLDLHRDS EREAIEVFIR SSFKDVDHLF QKELAAQLEK KRDDFCKQNQ
410 420 430 440 450
EASSDRCSAL LQVIFSPLEE EVKAGIYSKP GGYRLFVQKL QDLKKKYYEE
460 470 480 490 500
PRKGIQAEEI LQTYLKSKES MTDAILQTDQ TLTEKEKEIE VERVKAESAQ
510 520 530 540 550
ASAKMLQEMQ RKNEQMMEQK ERSYQEHLKQ LTEKMENDRV QLLKEQERTL
560 570 580 590
ALKLQEQEQL LKEGFQKESR IMKNEIQDLQ TKMRRRKACT IS
Length:592
Mass (Da):67,931
Last modified:November 2, 2010 - v2
Checksum:i3A06741218360732
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti78 – 781I → V.
Corresponds to variant rs1048401 [ dbSNP | Ensembl ].
VAR_033950
Natural varianti166 – 1661E → D.
Corresponds to variant rs17130717 [ dbSNP | Ensembl ].
VAR_033951
Natural varianti349 – 3491T → S.3 Publications
Corresponds to variant rs1048425 [ dbSNP | Ensembl ].
VAR_014849
Natural varianti409 – 4091A → G.3 Publications
Corresponds to variant rs1048443 [ dbSNP | Ensembl ].
VAR_046550

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55542 mRNA. Translation: AAA35871.1.
BT006847 mRNA. Translation: AAP35493.1.
AK291783 mRNA. Translation: BAF84472.1.
AL160008 Genomic DNA. Translation: CAI22972.1.
CH471097 Genomic DNA. Translation: EAW73148.1.
CH471097 Genomic DNA. Translation: EAW73150.1.
BC002666 mRNA. Translation: AAH02666.1.
CCDSiCCDS718.1.
PIRiA41268.
RefSeqiNP_002044.2. NM_002053.2.
UniGeneiHs.62661.

Genome annotation databases

EnsembliENST00000370473; ENSP00000359504; ENSG00000117228.
GeneIDi2633.
KEGGihsa:2633.
UCSCiuc001dmx.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55542 mRNA. Translation: AAA35871.1.
BT006847 mRNA. Translation: AAP35493.1.
AK291783 mRNA. Translation: BAF84472.1.
AL160008 Genomic DNA. Translation: CAI22972.1.
CH471097 Genomic DNA. Translation: EAW73148.1.
CH471097 Genomic DNA. Translation: EAW73150.1.
BC002666 mRNA. Translation: AAH02666.1.
CCDSiCCDS718.1.
PIRiA41268.
RefSeqiNP_002044.2. NM_002053.2.
UniGeneiHs.62661.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DG3X-ray1.80A1-592[»]
1F5NX-ray1.70A1-592[»]
2B8WX-ray2.22A/B1-317[»]
2B92X-ray3.20A/B1-317[»]
2BC9X-ray2.80A1-317[»]
2D4HX-ray2.90A/B1-317[»]
DisProtiDP00313.
ProteinModelPortaliP32455.
SMRiP32455. Positions 7-583.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108903. 18 interactions.
DIPiDIP-60423N.
IntActiP32455. 1 interaction.
MINTiMINT-4718320.
STRINGi9606.ENSP00000359504.

PTM databases

iPTMnetiP32455.
PhosphoSiteiP32455.
SwissPalmiP32455.

Polymorphism and mutation databases

BioMutaiGBP1.
DMDMi311033383.

Proteomic databases

EPDiP32455.
MaxQBiP32455.
PaxDbiP32455.
PeptideAtlasiP32455.
PRIDEiP32455.

Protocols and materials databases

DNASUi2633.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000370473; ENSP00000359504; ENSG00000117228.
GeneIDi2633.
KEGGihsa:2633.
UCSCiuc001dmx.3. human.

Organism-specific databases

CTDi2633.
GeneCardsiGBP1.
H-InvDBHIX0018119.
HIX0200036.
HGNCiHGNC:4182. GBP1.
HPAiCAB015450.
MIMi600411. gene.
neXtProtiNX_P32455.
PharmGKBiPA28596.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2037. Eukaryota.
ENOG410XR6Z. LUCA.
GeneTreeiENSGT00550000074475.
HOGENOMiHOG000266974.
HOVERGENiHBG001979.
InParanoidiP32455.
OMAiGFQKESR.
OrthoDBiEOG091G03P3.
PhylomeDBiP32455.
TreeFamiTF331602.

Enzyme and pathway databases

ReactomeiR-HSA-877300. Interferon gamma signaling.

Miscellaneous databases

ChiTaRSiGBP1. human.
EvolutionaryTraceiP32455.
GeneWikiiGBP1.
GenomeRNAii2633.
PROiP32455.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000117228.
CleanExiHS_GBP1.
GenevisibleiP32455. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR030386. G_GB1_RHD3_dom.
IPR003191. Guanylate-bd_C.
IPR015894. Guanylate-bd_N.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF02263. GBP. 1 hit.
PF02841. GBP_C. 1 hit.
[Graphical view]
SUPFAMiSSF48340. SSF48340. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51715. G_GB1_RHD3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGBP1_HUMAN
AccessioniPrimary (citable) accession number: P32455
Secondary accession number(s): D3DT26, Q5T8M1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: November 2, 2010
Last modified: September 7, 2016
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.