P32454 (APE2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminopeptidase 2, mitochondrial
Short name=AP-II
Short name=Aminopeptidase II
EC=3.4.11.-

Alternative name(s):
YscII

Gene names

Name:	APE2
Synonyms:	LAP1
Ordered Locus Names:	YKL157W
ORF Names:	YKL158W, YKL611, YKL612

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	952 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the cellular supply of leucine from externally offered leucine-containing dipeptide substrates.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Periplasm. Cytoplasm. Mitochondrion Ref.6 Ref.8 Ref.9 Ref.11.
Miscellaneous	Present with 2910 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the peptidase M1 family.
Sequence caution	The sequence AAS56682.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA45403.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA81496.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA81497.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA81497.1 differs from that shown. Reason: Frameshift at position 935. The sequence CAA81999.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA81999.1 differs from that shown. Reason: Frameshift at position 935. The sequence CAA82000.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm Mitochondrion Periplasm
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Aminopeptidase Hydrolase Metalloprotease Protease
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptide catabolic process Inferred from direct assay PubMed 6352682. Source: SGD proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell wall-bounded periplasmic space Inferred from direct assay Ref.6. Source: SGD extracellular region Inferred from direct assay Ref.6. Source: SGD mitochondrion Inferred from direct assay Ref.8PubMed 16823961. Source: SGD
Molecular_function	metalloaminopeptidase activity Inferred from direct assay PubMed 6352682. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 52	52	Mitochondrion Potential
	Chain	53 – 952	900	Aminopeptidase 2, mitochondrial		PRO_0000095104
Regions
	Region	360 – 364	5	Substrate binding By similarity
Sites
	Active site	397	1	Proton acceptor By similarity
	Metal binding	396	1	Zinc; catalytic By similarity
	Metal binding	400	1	Zinc; catalytic By similarity
	Metal binding	419	1	Zinc; catalytic By similarity
	Binding site	228	1	Substrate By similarity
	Site	482	1	Transition state stabilizer By similarity
Amino acid modifications
	Modified residue	606	1	Phosphothreonine Ref.12
	Glycosylation	381	1	N-linked (GlcNAc...) Potential
	Glycosylation	713	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	73	1	R → A in CAA45403. Ref.5
	Sequence conflict	92 – 93	2	LL → FI in CAA45403. Ref.5
	Sequence conflict	255	1	D → V in CAA45403. Ref.5
	Sequence conflict	533	1	D → E in CAA45403. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P32454 [UniParc]. Last modified September 21, 2011. Version 4. Checksum: 391C068DF4A18C2D	FASTA	952	107,755
	10 20 30 40 50 60 MPIVRWLLLK SAVRGSSLIG KAHPCLRSIA AHPRYLSNVY SPPAGVSRSL RINVMWKQSK 70 80 90 100 110 120 LTPPRFVKIM NRRPLFTETS HACAKCQKTS QLLNKTPNRE ILPDNVVPLH YDLTVEPDFK 130 140 150 160 170 180 TFKFEGSVKI ELKINNPAID TVTLNTVDTD IHSAKIGDVT SSEIISEEEQ QVTTFAFPKG 190 200 210 220 230 240 TMSSFKGNAF LDIKFTGILN DNMAGFYRAK YEDKLTGETK YMATTQMEPT DARRAFPCFD 250 260 270 280 290 300 EPNLKASFAI TLVSDPSLTH LSNMDVKNEY VKDGKKVTLF NTTPKMSTYL VAFIVAELKY 310 320 330 340 350 360 VESKNFRIPV RVYATPGNEK HGQFAADLTA KTLAFFEKTF GIQYPLPKMD NVAVHEFSAG 370 380 390 400 410 420 AMENWGLVTY RVVDLLLDKD NSTLDRIQRV AEVVQHELAH QWFGNLVTMD WWEGLWLNEG 430 440 450 460 470 480 FATWMSWYSC NEFQPEWKVW EQYVTDTLQH ALSLDSLRSS HPIEVPVKKA DEINQIFDAI 490 500 510 520 530 540 SYSKGASLLR MISKWLGEET FIKGVSQYLN KFKYGNAKTE DLWDALADAS GKDVRSVMNI 550 560 570 580 590 600 WTKKVGFPVI SVSEDGNGKI TFRQNRYLST ADVKPDEDKT IYPVFLALKT KNGVDSSVVL 610 620 630 640 650 660 SERSKTIELE DPTFFKVNSE QSGIYITSYT DERWAKLGQQ ADLLSVEDRV GLVADVKTLS 670 680 690 700 710 720 ASGYTSTTNF LNLVSKWNNE KSFVVWDQII NSISSMKSTW LFEPKETQDA LDNFTKQLIS 730 740 750 760 770 780 GMTHHLGWEF KSSDSFSTQR LKVTMFGAAC AARDADVEKA ALKMFTDYCS GNKEAIPALI 790 800 810 820 830 840 KPIVFNTVAR VGGAENYEKV YKIYLDPISN DEKLAALRSL GRFKEPKLLE RTLGYLFDGT 850 860 870 880 890 900 VLNQDIYIPM QGMRAHQEGV EALWNWVKKN WDELVKRLPP GLSMLGSVVT LGTSGFTSMQ 910 920 930 940 950 KIDEIKKFFA TKSTKGFDQS LAQSLDTITS KAQWVNRDRD VVNKYLKENG YY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae." Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:S35-S40(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 935. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-93. Strain: ATCC 204508 / S288c.
[5]	"Molecular cloning of soluble aminopeptidases from Saccharomyces cerevisiae. Sequence analysis of aminopeptidase yscII, a putative zinc-metallopeptidase." Garcia-Alvarez N., Cueva R., Suarez-Rendueles P. Eur. J. Biochem. 202:993-1002(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 35-952. Strain: ATCC 204510 / AB320.
[6]	"External and internal forms of yeast aminopeptidase II." Frey J., Roehm K.-H. Eur. J. Biochem. 97:169-173(1979) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[7]	"Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast." Davis C.A., Grate L., Spingola M., Ares M. Jr. Nucleic Acids Res. 28:1700-1706(2000) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF INTRON.
[8]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
[9]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"The yeast mitochondrial proteome, a study of fermentative and respiratory growth." Ohlmeier S., Kastaniotis A.J., Hiltunen J.K., Bergmann U. J. Biol. Chem. 279:3956-3979(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-606, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z26877 Genomic DNA. Translation: CAA81496.1. Sequence problems. Z26877 Genomic DNA. Translation: CAA81497.1. Sequence problems. Z28157 Genomic DNA. Translation: CAA81999.1. Sequence problems. Z28158 Genomic DNA. Translation: CAA82000.1. Sequence problems. AY558356 Genomic DNA. Translation: AAS56682.1. Sequence problems. X63998 Genomic DNA. Translation: CAA45403.1. Sequence problems. BK006944 Genomic DNA. Translation: DAA09007.2.
PIR	S37793. S37794.
RefSeq	NP_012765.3. NM_001179723.2.
3D structure databases
ProteinModelPortal	P32454.
SMR	P32454. Positions 102-949.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4391N.
IntAct	P32454. 53 interactions.
MINT	MINT-503293.
STRING	4932.YKL157W.
Protein family/group databases
MEROPS	M01.006.
Proteomic databases
PaxDb	P32454.
PeptideAtlas	P32454.
PRIDE	P32454.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL157W; YKL157W; YKL157W.
GeneID	853699.
KEGG	sce:YKL157W.
Organism-specific databases
CYGD	YKL157w.
SGD	S000001640. APE2.
Phylogenomic databases
eggNOG	COG0308.
GeneTree	ENSGT00680000099758.
HOGENOM	HOG000106482.
KO	K13721.
OMA	FFATKST.
OrthoDB	EOG42VCQG.
Gene expression databases
Genevestigator	P32454.
GermOnline	YKL157W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR024571. DUF3358. IPR001930. Peptidase_M1. IPR014782. Peptidase_M1_N. [Graphical view]
PANTHER	PTHR11533. PTHR11533. 1 hit.
Pfam	PF11838. DUF3358. 1 hit. PF01433. Peptidase_M1. 1 hit. [Graphical view]
PRINTS	PR00756. ALADIPTASE.
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974687.

Entry information

Entry name

APE2_YEAST

Accession

Primary (citable) accession number: P32454
Secondary accession number(s): D6VX41, P36055

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	September 21, 2011
Last modified:	May 1, 2013
This is version 129 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program