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P32454

- APE2_YEAST

UniProt

P32454 - APE2_YEAST

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Protein

Aminopeptidase 2, mitochondrial

Gene

APE2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the cellular supply of leucine from externally offered leucine-containing dipeptide substrates.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei228 – 2281SubstrateBy similarity
Metal bindingi396 – 3961Zinc; catalyticPROSITE-ProRule annotation
Active sitei397 – 3971Proton acceptorPROSITE-ProRule annotation
Metal bindingi400 – 4001Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi419 – 4191Zinc; catalyticPROSITE-ProRule annotation
Sitei482 – 4821Transition state stabilizerBy similarity

GO - Molecular functioni

  1. metalloaminopeptidase activity Source: SGD
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. peptide catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YKL157W-MONOMER.
ReactomeiREACT_189060. Antigen processing: Ubiquitination & Proteasome degradation.

Protein family/group databases

MEROPSiM01.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase 2, mitochondrial (EC:3.4.11.-)
Short name:
AP-II
Short name:
Aminopeptidase II
Alternative name(s):
YscII
Gene namesi
Name:APE2
Synonyms:LAP1
Ordered Locus Names:YKL157W
ORF Names:YKL158W, YKL611, YKL612
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL157w.
SGDiS000001640. APE2.

Subcellular locationi

GO - Cellular componenti

  1. cell wall-bounded periplasmic space Source: SGD
  2. extracellular region Source: SGD
  3. mitochondrion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5252MitochondrionSequence AnalysisAdd
BLAST
Chaini53 – 952900Aminopeptidase 2, mitochondrialPRO_0000095104Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi381 – 3811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi713 – 7131N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP32454.
PaxDbiP32454.
PeptideAtlasiP32454.
PRIDEiP32454.

Expressioni

Gene expression databases

GenevestigatoriP32454.

Interactioni

Protein-protein interaction databases

BioGridi33980. 72 interactions.
DIPiDIP-4391N.
IntActiP32454. 52 interactions.
MINTiMINT-503293.
STRINGi4932.YKL157W.

Structurei

3D structure databases

ProteinModelPortaliP32454.
SMRiP32454. Positions 102-946.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni360 – 3645Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0308.
GeneTreeiENSGT00760000119082.
HOGENOMiHOG000106482.
InParanoidiP32454.
KOiK13721.
OMAiRTVQQCC.
OrthoDBiEOG75TMMT.

Family and domain databases

InterProiIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32454-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPIVRWLLLK SAVRGSSLIG KAHPCLRSIA AHPRYLSNVY SPPAGVSRSL
60 70 80 90 100
RINVMWKQSK LTPPRFVKIM NRRPLFTETS HACAKCQKTS QLLNKTPNRE
110 120 130 140 150
ILPDNVVPLH YDLTVEPDFK TFKFEGSVKI ELKINNPAID TVTLNTVDTD
160 170 180 190 200
IHSAKIGDVT SSEIISEEEQ QVTTFAFPKG TMSSFKGNAF LDIKFTGILN
210 220 230 240 250
DNMAGFYRAK YEDKLTGETK YMATTQMEPT DARRAFPCFD EPNLKASFAI
260 270 280 290 300
TLVSDPSLTH LSNMDVKNEY VKDGKKVTLF NTTPKMSTYL VAFIVAELKY
310 320 330 340 350
VESKNFRIPV RVYATPGNEK HGQFAADLTA KTLAFFEKTF GIQYPLPKMD
360 370 380 390 400
NVAVHEFSAG AMENWGLVTY RVVDLLLDKD NSTLDRIQRV AEVVQHELAH
410 420 430 440 450
QWFGNLVTMD WWEGLWLNEG FATWMSWYSC NEFQPEWKVW EQYVTDTLQH
460 470 480 490 500
ALSLDSLRSS HPIEVPVKKA DEINQIFDAI SYSKGASLLR MISKWLGEET
510 520 530 540 550
FIKGVSQYLN KFKYGNAKTE DLWDALADAS GKDVRSVMNI WTKKVGFPVI
560 570 580 590 600
SVSEDGNGKI TFRQNRYLST ADVKPDEDKT IYPVFLALKT KNGVDSSVVL
610 620 630 640 650
SERSKTIELE DPTFFKVNSE QSGIYITSYT DERWAKLGQQ ADLLSVEDRV
660 670 680 690 700
GLVADVKTLS ASGYTSTTNF LNLVSKWNNE KSFVVWDQII NSISSMKSTW
710 720 730 740 750
LFEPKETQDA LDNFTKQLIS GMTHHLGWEF KSSDSFSTQR LKVTMFGAAC
760 770 780 790 800
AARDADVEKA ALKMFTDYCS GNKEAIPALI KPIVFNTVAR VGGAENYEKV
810 820 830 840 850
YKIYLDPISN DEKLAALRSL GRFKEPKLLE RTLGYLFDGT VLNQDIYIPM
860 870 880 890 900
QGMRAHQEGV EALWNWVKKN WDELVKRLPP GLSMLGSVVT LGTSGFTSMQ
910 920 930 940 950
KIDEIKKFFA TKSTKGFDQS LAQSLDTITS KAQWVNRDRD VVNKYLKENG

YY
Length:952
Mass (Da):107,755
Last modified:September 21, 2011 - v4
Checksum:i391C068DF4A18C2D
GO

Sequence cautioni

The sequence AAS56682.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAA45403.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAA81496.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAA81497.1 differs from that shown. Reason: Frameshift at position 935. Curated
The sequence CAA81497.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAA81999.1 differs from that shown. Reason: Frameshift at position 935. Curated
The sequence CAA81999.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAA82000.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti73 – 731R → A in CAA45403. (PubMed:1765107)Curated
Sequence conflicti92 – 932LL → FI in CAA45403. (PubMed:1765107)Curated
Sequence conflicti255 – 2551D → V in CAA45403. (PubMed:1765107)Curated
Sequence conflicti533 – 5331D → E in CAA45403. (PubMed:1765107)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26877 Genomic DNA. Translation: CAA81496.1. Sequence problems.
Z26877 Genomic DNA. Translation: CAA81497.1. Sequence problems.
Z28157 Genomic DNA. Translation: CAA81999.1. Sequence problems.
Z28158 Genomic DNA. Translation: CAA82000.1. Sequence problems.
AY558356 Genomic DNA. Translation: AAS56682.1. Sequence problems.
X63998 Genomic DNA. Translation: CAA45403.1. Sequence problems.
BK006944 Genomic DNA. Translation: DAA09007.2.
PIRiS37793.
S37794.
RefSeqiNP_012765.3. NM_001179723.2.

Genome annotation databases

EnsemblFungiiYKL157W; YKL157W; YKL157W.
GeneIDi853699.
KEGGisce:YKL157W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26877 Genomic DNA. Translation: CAA81496.1 . Sequence problems.
Z26877 Genomic DNA. Translation: CAA81497.1 . Sequence problems.
Z28157 Genomic DNA. Translation: CAA81999.1 . Sequence problems.
Z28158 Genomic DNA. Translation: CAA82000.1 . Sequence problems.
AY558356 Genomic DNA. Translation: AAS56682.1 . Sequence problems.
X63998 Genomic DNA. Translation: CAA45403.1 . Sequence problems.
BK006944 Genomic DNA. Translation: DAA09007.2 .
PIRi S37793.
S37794.
RefSeqi NP_012765.3. NM_001179723.2.

3D structure databases

ProteinModelPortali P32454.
SMRi P32454. Positions 102-946.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33980. 72 interactions.
DIPi DIP-4391N.
IntActi P32454. 52 interactions.
MINTi MINT-503293.
STRINGi 4932.YKL157W.

Protein family/group databases

MEROPSi M01.006.

Proteomic databases

MaxQBi P32454.
PaxDbi P32454.
PeptideAtlasi P32454.
PRIDEi P32454.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL157W ; YKL157W ; YKL157W .
GeneIDi 853699.
KEGGi sce:YKL157W.

Organism-specific databases

CYGDi YKL157w.
SGDi S000001640. APE2.

Phylogenomic databases

eggNOGi COG0308.
GeneTreei ENSGT00760000119082.
HOGENOMi HOG000106482.
InParanoidi P32454.
KOi K13721.
OMAi RTVQQCC.
OrthoDBi EOG75TMMT.

Enzyme and pathway databases

BioCyci YEAST:YKL157W-MONOMER.
Reactomei REACT_189060. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

NextBioi 974687.

Gene expression databases

Genevestigatori P32454.

Family and domain databases

InterProi IPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view ]
PANTHERi PTHR11533. PTHR11533. 1 hit.
Pfami PF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view ]
PRINTSi PR00756. ALADIPTASE.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae."
    Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
    Yeast 10:S35-S40(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 935.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-93.
    Strain: ATCC 204508 / S288c.
  5. "Molecular cloning of soluble aminopeptidases from Saccharomyces cerevisiae. Sequence analysis of aminopeptidase yscII, a putative zinc-metallopeptidase."
    Garcia-Alvarez N., Cueva R., Suarez-Rendueles P.
    Eur. J. Biochem. 202:993-1002(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 35-952.
    Strain: ATCC 204510 / AB320.
  6. "External and internal forms of yeast aminopeptidase II."
    Frey J., Roehm K.-H.
    Eur. J. Biochem. 97:169-173(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. "Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast."
    Davis C.A., Grate L., Spingola M., Ares M. Jr.
    Nucleic Acids Res. 28:1700-1706(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF INTRON.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "The yeast mitochondrial proteome, a study of fermentative and respiratory growth."
    Ohlmeier S., Kastaniotis A.J., Hiltunen J.K., Bergmann U.
    J. Biol. Chem. 279:3956-3979(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAPE2_YEAST
AccessioniPrimary (citable) accession number: P32454
Secondary accession number(s): D6VX41, P36055
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: September 21, 2011
Last modified: November 26, 2014
This is version 141 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2910 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3