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P32454

- APE2_YEAST

UniProt

P32454 - APE2_YEAST

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Protein
Aminopeptidase 2, mitochondrial
Gene
APE2, LAP1, YKL157W, YKL158W, YKL611, YKL612
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the cellular supply of leucine from externally offered leucine-containing dipeptide substrates.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei228 – 2281Substrate By similarity
Metal bindingi396 – 3961Zinc; catalytic By similarity
Active sitei397 – 3971Proton acceptor By similarity
Metal bindingi400 – 4001Zinc; catalytic By similarity
Metal bindingi419 – 4191Zinc; catalytic By similarity
Sitei482 – 4821Transition state stabilizer By similarity

GO - Molecular functioni

  1. metalloaminopeptidase activity Source: SGD
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. peptide catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YKL157W-MONOMER.
ReactomeiREACT_189060. Antigen processing: Ubiquitination & Proteasome degradation.

Protein family/group databases

MEROPSiM01.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase 2, mitochondrial (EC:3.4.11.-)
Short name:
AP-II
Short name:
Aminopeptidase II
Alternative name(s):
YscII
Gene namesi
Name:APE2
Synonyms:LAP1
Ordered Locus Names:YKL157W
ORF Names:YKL158W, YKL611, YKL612
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL157w.
SGDiS000001640. APE2.

Subcellular locationi

Periplasm. Cytoplasm. Mitochondrion 4 Publications

GO - Cellular componenti

  1. cell wall-bounded periplasmic space Source: SGD
  2. extracellular region Source: SGD
  3. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5252Mitochondrion Reviewed prediction
Add
BLAST
Chaini53 – 952900Aminopeptidase 2, mitochondrial
PRO_0000095104Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi381 – 3811N-linked (GlcNAc...) Reviewed prediction
Glycosylationi713 – 7131N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP32454.
PaxDbiP32454.
PeptideAtlasiP32454.
PRIDEiP32454.

Expressioni

Gene expression databases

GenevestigatoriP32454.

Interactioni

Protein-protein interaction databases

BioGridi33980. 71 interactions.
DIPiDIP-4391N.
IntActiP32454. 52 interactions.
MINTiMINT-503293.
STRINGi4932.YKL157W.

Structurei

3D structure databases

ProteinModelPortaliP32454.
SMRiP32454. Positions 102-946.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni360 – 3645Substrate binding By similarity

Sequence similaritiesi

Belongs to the peptidase M1 family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0308.
GeneTreeiENSGT00740000114965.
HOGENOMiHOG000106482.
KOiK13721.
OMAiRTVQQCC.
OrthoDBiEOG75TMMT.

Family and domain databases

InterProiIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32454-1 [UniParc]FASTAAdd to Basket

« Hide

MPIVRWLLLK SAVRGSSLIG KAHPCLRSIA AHPRYLSNVY SPPAGVSRSL    50
RINVMWKQSK LTPPRFVKIM NRRPLFTETS HACAKCQKTS QLLNKTPNRE 100
ILPDNVVPLH YDLTVEPDFK TFKFEGSVKI ELKINNPAID TVTLNTVDTD 150
IHSAKIGDVT SSEIISEEEQ QVTTFAFPKG TMSSFKGNAF LDIKFTGILN 200
DNMAGFYRAK YEDKLTGETK YMATTQMEPT DARRAFPCFD EPNLKASFAI 250
TLVSDPSLTH LSNMDVKNEY VKDGKKVTLF NTTPKMSTYL VAFIVAELKY 300
VESKNFRIPV RVYATPGNEK HGQFAADLTA KTLAFFEKTF GIQYPLPKMD 350
NVAVHEFSAG AMENWGLVTY RVVDLLLDKD NSTLDRIQRV AEVVQHELAH 400
QWFGNLVTMD WWEGLWLNEG FATWMSWYSC NEFQPEWKVW EQYVTDTLQH 450
ALSLDSLRSS HPIEVPVKKA DEINQIFDAI SYSKGASLLR MISKWLGEET 500
FIKGVSQYLN KFKYGNAKTE DLWDALADAS GKDVRSVMNI WTKKVGFPVI 550
SVSEDGNGKI TFRQNRYLST ADVKPDEDKT IYPVFLALKT KNGVDSSVVL 600
SERSKTIELE DPTFFKVNSE QSGIYITSYT DERWAKLGQQ ADLLSVEDRV 650
GLVADVKTLS ASGYTSTTNF LNLVSKWNNE KSFVVWDQII NSISSMKSTW 700
LFEPKETQDA LDNFTKQLIS GMTHHLGWEF KSSDSFSTQR LKVTMFGAAC 750
AARDADVEKA ALKMFTDYCS GNKEAIPALI KPIVFNTVAR VGGAENYEKV 800
YKIYLDPISN DEKLAALRSL GRFKEPKLLE RTLGYLFDGT VLNQDIYIPM 850
QGMRAHQEGV EALWNWVKKN WDELVKRLPP GLSMLGSVVT LGTSGFTSMQ 900
KIDEIKKFFA TKSTKGFDQS LAQSLDTITS KAQWVNRDRD VVNKYLKENG 950
YY 952
Length:952
Mass (Da):107,755
Last modified:September 21, 2011 - v4
Checksum:i391C068DF4A18C2D
GO

Sequence cautioni

The sequence CAA81497.1 differs from that shown. Reason: Frameshift at position 935.
The sequence CAA81999.1 differs from that shown. Reason: Frameshift at position 935.
The sequence AAS56682.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAA45403.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAA81496.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAA81497.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAA81999.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAA82000.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti73 – 731R → A in CAA45403. 1 Publication
Sequence conflicti92 – 932LL → FI in CAA45403. 1 Publication
Sequence conflicti255 – 2551D → V in CAA45403. 1 Publication
Sequence conflicti533 – 5331D → E in CAA45403. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z26877 Genomic DNA. Translation: CAA81496.1. Sequence problems.
Z26877 Genomic DNA. Translation: CAA81497.1. Sequence problems.
Z28157 Genomic DNA. Translation: CAA81999.1. Sequence problems.
Z28158 Genomic DNA. Translation: CAA82000.1. Sequence problems.
AY558356 Genomic DNA. Translation: AAS56682.1. Sequence problems.
X63998 Genomic DNA. Translation: CAA45403.1. Sequence problems.
BK006944 Genomic DNA. Translation: DAA09007.2.
PIRiS37793.
S37794.
RefSeqiNP_012765.3. NM_001179723.2.

Genome annotation databases

EnsemblFungiiYKL157W; YKL157W; YKL157W.
GeneIDi853699.
KEGGisce:YKL157W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z26877 Genomic DNA. Translation: CAA81496.1 . Sequence problems.
Z26877 Genomic DNA. Translation: CAA81497.1 . Sequence problems.
Z28157 Genomic DNA. Translation: CAA81999.1 . Sequence problems.
Z28158 Genomic DNA. Translation: CAA82000.1 . Sequence problems.
AY558356 Genomic DNA. Translation: AAS56682.1 . Sequence problems.
X63998 Genomic DNA. Translation: CAA45403.1 . Sequence problems.
BK006944 Genomic DNA. Translation: DAA09007.2 .
PIRi S37793.
S37794.
RefSeqi NP_012765.3. NM_001179723.2.

3D structure databases

ProteinModelPortali P32454.
SMRi P32454. Positions 102-946.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33980. 71 interactions.
DIPi DIP-4391N.
IntActi P32454. 52 interactions.
MINTi MINT-503293.
STRINGi 4932.YKL157W.

Protein family/group databases

MEROPSi M01.006.

Proteomic databases

MaxQBi P32454.
PaxDbi P32454.
PeptideAtlasi P32454.
PRIDEi P32454.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL157W ; YKL157W ; YKL157W .
GeneIDi 853699.
KEGGi sce:YKL157W.

Organism-specific databases

CYGDi YKL157w.
SGDi S000001640. APE2.

Phylogenomic databases

eggNOGi COG0308.
GeneTreei ENSGT00740000114965.
HOGENOMi HOG000106482.
KOi K13721.
OMAi RTVQQCC.
OrthoDBi EOG75TMMT.

Enzyme and pathway databases

BioCyci YEAST:YKL157W-MONOMER.
Reactomei REACT_189060. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

NextBioi 974687.

Gene expression databases

Genevestigatori P32454.

Family and domain databases

InterProi IPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view ]
PANTHERi PTHR11533. PTHR11533. 1 hit.
Pfami PF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view ]
PRINTSi PR00756. ALADIPTASE.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae."
    Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
    Yeast 10:S35-S40(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 935.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-93.
    Strain: ATCC 204508 / S288c.
  5. "Molecular cloning of soluble aminopeptidases from Saccharomyces cerevisiae. Sequence analysis of aminopeptidase yscII, a putative zinc-metallopeptidase."
    Garcia-Alvarez N., Cueva R., Suarez-Rendueles P.
    Eur. J. Biochem. 202:993-1002(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 35-952.
    Strain: ATCC 204510 / AB320.
  6. "External and internal forms of yeast aminopeptidase II."
    Frey J., Roehm K.-H.
    Eur. J. Biochem. 97:169-173(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. "Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast."
    Davis C.A., Grate L., Spingola M., Ares M. Jr.
    Nucleic Acids Res. 28:1700-1706(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF INTRON.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "The yeast mitochondrial proteome, a study of fermentative and respiratory growth."
    Ohlmeier S., Kastaniotis A.J., Hiltunen J.K., Bergmann U.
    J. Biol. Chem. 279:3956-3979(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAPE2_YEAST
AccessioniPrimary (citable) accession number: P32454
Secondary accession number(s): D6VX41, P36055
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: September 21, 2011
Last modified: September 3, 2014
This is version 138 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2910 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

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