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P32454 (APE2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aminopeptidase 2, mitochondrial

Short name=AP-II
Short name=Aminopeptidase II
EC=3.4.11.-
Alternative name(s):
YscII
Gene names
Name:APE2
Synonyms:LAP1
Ordered Locus Names:YKL157W
ORF Names:YKL158W, YKL611, YKL612
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length952 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the cellular supply of leucine from externally offered leucine-containing dipeptide substrates.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subcellular location

Periplasm. Cytoplasm. Mitochondrion Ref.6 Ref.8 Ref.9 Ref.11.

Miscellaneous

Present with 2910 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the peptidase M1 family.

Sequence caution

The sequence AAS56682.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAA45403.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAA81496.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAA81497.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAA81497.1 differs from that shown. Reason: Frameshift at position 935.

The sequence CAA81999.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAA81999.1 differs from that shown. Reason: Frameshift at position 935.

The sequence CAA82000.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5252Mitochondrion Potential
Chain53 – 952900Aminopeptidase 2, mitochondrial
PRO_0000095104

Regions

Region360 – 3645Substrate binding By similarity

Sites

Active site3971Proton acceptor By similarity
Metal binding3961Zinc; catalytic By similarity
Metal binding4001Zinc; catalytic By similarity
Metal binding4191Zinc; catalytic By similarity
Binding site2281Substrate By similarity
Site4821Transition state stabilizer By similarity

Amino acid modifications

Glycosylation3811N-linked (GlcNAc...) Potential
Glycosylation7131N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict731R → A in CAA45403. Ref.5
Sequence conflict92 – 932LL → FI in CAA45403. Ref.5
Sequence conflict2551D → V in CAA45403. Ref.5
Sequence conflict5331D → E in CAA45403. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P32454 [UniParc].

Last modified September 21, 2011. Version 4.
Checksum: 391C068DF4A18C2D

FASTA952107,755
        10         20         30         40         50         60 
MPIVRWLLLK SAVRGSSLIG KAHPCLRSIA AHPRYLSNVY SPPAGVSRSL RINVMWKQSK 

        70         80         90        100        110        120 
LTPPRFVKIM NRRPLFTETS HACAKCQKTS QLLNKTPNRE ILPDNVVPLH YDLTVEPDFK 

       130        140        150        160        170        180 
TFKFEGSVKI ELKINNPAID TVTLNTVDTD IHSAKIGDVT SSEIISEEEQ QVTTFAFPKG 

       190        200        210        220        230        240 
TMSSFKGNAF LDIKFTGILN DNMAGFYRAK YEDKLTGETK YMATTQMEPT DARRAFPCFD 

       250        260        270        280        290        300 
EPNLKASFAI TLVSDPSLTH LSNMDVKNEY VKDGKKVTLF NTTPKMSTYL VAFIVAELKY 

       310        320        330        340        350        360 
VESKNFRIPV RVYATPGNEK HGQFAADLTA KTLAFFEKTF GIQYPLPKMD NVAVHEFSAG 

       370        380        390        400        410        420 
AMENWGLVTY RVVDLLLDKD NSTLDRIQRV AEVVQHELAH QWFGNLVTMD WWEGLWLNEG 

       430        440        450        460        470        480 
FATWMSWYSC NEFQPEWKVW EQYVTDTLQH ALSLDSLRSS HPIEVPVKKA DEINQIFDAI 

       490        500        510        520        530        540 
SYSKGASLLR MISKWLGEET FIKGVSQYLN KFKYGNAKTE DLWDALADAS GKDVRSVMNI 

       550        560        570        580        590        600 
WTKKVGFPVI SVSEDGNGKI TFRQNRYLST ADVKPDEDKT IYPVFLALKT KNGVDSSVVL 

       610        620        630        640        650        660 
SERSKTIELE DPTFFKVNSE QSGIYITSYT DERWAKLGQQ ADLLSVEDRV GLVADVKTLS 

       670        680        690        700        710        720 
ASGYTSTTNF LNLVSKWNNE KSFVVWDQII NSISSMKSTW LFEPKETQDA LDNFTKQLIS 

       730        740        750        760        770        780 
GMTHHLGWEF KSSDSFSTQR LKVTMFGAAC AARDADVEKA ALKMFTDYCS GNKEAIPALI 

       790        800        810        820        830        840 
KPIVFNTVAR VGGAENYEKV YKIYLDPISN DEKLAALRSL GRFKEPKLLE RTLGYLFDGT 

       850        860        870        880        890        900 
VLNQDIYIPM QGMRAHQEGV EALWNWVKKN WDELVKRLPP GLSMLGSVVT LGTSGFTSMQ 

       910        920        930        940        950 
KIDEIKKFFA TKSTKGFDQS LAQSLDTITS KAQWVNRDRD VVNKYLKENG YY 

« Hide

References

« Hide 'large scale' references
[1]"DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae."
Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
Yeast 10:S35-S40(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 935.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-93.
Strain: ATCC 204508 / S288c.
[5]"Molecular cloning of soluble aminopeptidases from Saccharomyces cerevisiae. Sequence analysis of aminopeptidase yscII, a putative zinc-metallopeptidase."
Garcia-Alvarez N., Cueva R., Suarez-Rendueles P.
Eur. J. Biochem. 202:993-1002(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 35-952.
Strain: ATCC 204510 / AB320.
[6]"External and internal forms of yeast aminopeptidase II."
Frey J., Roehm K.-H.
Eur. J. Biochem. 97:169-173(1979) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[7]"Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast."
Davis C.A., Grate L., Spingola M., Ares M. Jr.
Nucleic Acids Res. 28:1700-1706(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF INTRON.
[8]"The proteome of Saccharomyces cerevisiae mitochondria."
Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C.
Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: ATCC 76625 / YPH499.
[9]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]"The yeast mitochondrial proteome, a study of fermentative and respiratory growth."
Ohlmeier S., Kastaniotis A.J., Hiltunen J.K., Bergmann U.
J. Biol. Chem. 279:3956-3979(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[12]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z26877 Genomic DNA. Translation: CAA81496.1. Sequence problems.
Z26877 Genomic DNA. Translation: CAA81497.1. Sequence problems.
Z28157 Genomic DNA. Translation: CAA81999.1. Sequence problems.
Z28158 Genomic DNA. Translation: CAA82000.1. Sequence problems.
AY558356 Genomic DNA. Translation: AAS56682.1. Sequence problems.
X63998 Genomic DNA. Translation: CAA45403.1. Sequence problems.
BK006944 Genomic DNA. Translation: DAA09007.2.
PIRS37793.
S37794.
RefSeqNP_012765.3. NM_001179723.2.

3D structure databases

ProteinModelPortalP32454.
SMRP32454. Positions 102-946.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33980. 71 interactions.
DIPDIP-4391N.
IntActP32454. 52 interactions.
MINTMINT-503293.
STRING4932.YKL157W.

Protein family/group databases

MEROPSM01.006.

Proteomic databases

PaxDbP32454.
PeptideAtlasP32454.
PRIDEP32454.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL157W; YKL157W; YKL157W.
GeneID853699.
KEGGsce:YKL157W.

Organism-specific databases

CYGDYKL157w.
SGDS000001640. APE2.

Phylogenomic databases

eggNOGCOG0308.
GeneTreeENSGT00740000114965.
HOGENOMHOG000106482.
KOK13721.
OMAKFQHKNA.
OrthoDBEOG75TMMT.

Enzyme and pathway databases

BioCycYEAST:YKL157W-MONOMER.

Gene expression databases

GenevestigatorP32454.

Family and domain databases

InterProIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERPTHR11533. PTHR11533. 1 hit.
PfamPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSPR00756. ALADIPTASE.
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974687.

Entry information

Entry nameAPE2_YEAST
AccessionPrimary (citable) accession number: P32454
Secondary accession number(s): D6VX41, P36055
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: September 21, 2011
Last modified: March 19, 2014
This is version 136 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries