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Protein

Aminopeptidase 2, mitochondrial

Gene

APE2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the cellular supply of leucine from externally offered leucine-containing dipeptide substrates.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei228SubstrateBy similarity1
Metal bindingi396Zinc; catalyticPROSITE-ProRule annotation1
Active sitei397Proton acceptorPROSITE-ProRule annotation1
Metal bindingi400Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi419Zinc; catalyticPROSITE-ProRule annotation1
Sitei482Transition state stabilizerBy similarity1

GO - Molecular functioni

  • metalloaminopeptidase activity Source: SGD
  • peptide binding Source: GO_Central
  • zinc ion binding Source: GO_Central

GO - Biological processi

  • peptide catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YKL157W-MONOMER.
ReactomeiR-SCE-1445148. Translocation of GLUT4 to the plasma membrane.
R-SCE-6798695. Neutrophil degranulation.
R-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.
R-SCE-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Protein family/group databases

MEROPSiM01.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase 2, mitochondrial (EC:3.4.11.-)
Short name:
AP-II
Short name:
Aminopeptidase II
Alternative name(s):
YscII
Gene namesi
Name:APE2
Synonyms:LAP1
Ordered Locus Names:YKL157W
ORF Names:YKL158W, YKL611, YKL612
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL157W.
SGDiS000001640. APE2.

Subcellular locationi

GO - Cellular componenti

  • cell wall-bounded periplasmic space Source: SGD
  • extracellular region Source: SGD
  • mitochondrion Source: UniProtKB-SubCell
  • plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 52MitochondrionSequence analysisAdd BLAST52
ChainiPRO_000009510453 – 952Aminopeptidase 2, mitochondrialAdd BLAST900

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi381N-linked (GlcNAc...)Sequence analysis1
Glycosylationi713N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP32454.
PRIDEiP32454.

PTM databases

iPTMnetiP32454.

Interactioni

Protein-protein interaction databases

BioGridi33980. 74 interactors.
DIPiDIP-4391N.
IntActiP32454. 52 interactors.
MINTiMINT-503293.

Structurei

3D structure databases

ProteinModelPortaliP32454.
SMRiP32454.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni360 – 364Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00760000119082.
HOGENOMiHOG000106482.
InParanoidiP32454.
KOiK13721.
OMAiHAMENDS.
OrthoDBiEOG092C19PD.

Family and domain databases

InterProiIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32454-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIVRWLLLK SAVRGSSLIG KAHPCLRSIA AHPRYLSNVY SPPAGVSRSL
60 70 80 90 100
RINVMWKQSK LTPPRFVKIM NRRPLFTETS HACAKCQKTS QLLNKTPNRE
110 120 130 140 150
ILPDNVVPLH YDLTVEPDFK TFKFEGSVKI ELKINNPAID TVTLNTVDTD
160 170 180 190 200
IHSAKIGDVT SSEIISEEEQ QVTTFAFPKG TMSSFKGNAF LDIKFTGILN
210 220 230 240 250
DNMAGFYRAK YEDKLTGETK YMATTQMEPT DARRAFPCFD EPNLKASFAI
260 270 280 290 300
TLVSDPSLTH LSNMDVKNEY VKDGKKVTLF NTTPKMSTYL VAFIVAELKY
310 320 330 340 350
VESKNFRIPV RVYATPGNEK HGQFAADLTA KTLAFFEKTF GIQYPLPKMD
360 370 380 390 400
NVAVHEFSAG AMENWGLVTY RVVDLLLDKD NSTLDRIQRV AEVVQHELAH
410 420 430 440 450
QWFGNLVTMD WWEGLWLNEG FATWMSWYSC NEFQPEWKVW EQYVTDTLQH
460 470 480 490 500
ALSLDSLRSS HPIEVPVKKA DEINQIFDAI SYSKGASLLR MISKWLGEET
510 520 530 540 550
FIKGVSQYLN KFKYGNAKTE DLWDALADAS GKDVRSVMNI WTKKVGFPVI
560 570 580 590 600
SVSEDGNGKI TFRQNRYLST ADVKPDEDKT IYPVFLALKT KNGVDSSVVL
610 620 630 640 650
SERSKTIELE DPTFFKVNSE QSGIYITSYT DERWAKLGQQ ADLLSVEDRV
660 670 680 690 700
GLVADVKTLS ASGYTSTTNF LNLVSKWNNE KSFVVWDQII NSISSMKSTW
710 720 730 740 750
LFEPKETQDA LDNFTKQLIS GMTHHLGWEF KSSDSFSTQR LKVTMFGAAC
760 770 780 790 800
AARDADVEKA ALKMFTDYCS GNKEAIPALI KPIVFNTVAR VGGAENYEKV
810 820 830 840 850
YKIYLDPISN DEKLAALRSL GRFKEPKLLE RTLGYLFDGT VLNQDIYIPM
860 870 880 890 900
QGMRAHQEGV EALWNWVKKN WDELVKRLPP GLSMLGSVVT LGTSGFTSMQ
910 920 930 940 950
KIDEIKKFFA TKSTKGFDQS LAQSLDTITS KAQWVNRDRD VVNKYLKENG

YY
Length:952
Mass (Da):107,755
Last modified:September 21, 2011 - v4
Checksum:i391C068DF4A18C2D
GO

Sequence cautioni

The sequence AAS56682 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA45403 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA81496 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA81497 differs from that shown. Reason: Frameshift at position 935.Curated
The sequence CAA81497 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA81999 differs from that shown. Reason: Frameshift at position 935.Curated
The sequence CAA81999 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA82000 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti73R → A in CAA45403 (PubMed:1765107).Curated1
Sequence conflicti92 – 93LL → FI in CAA45403 (PubMed:1765107).Curated2
Sequence conflicti255D → V in CAA45403 (PubMed:1765107).Curated1
Sequence conflicti533D → E in CAA45403 (PubMed:1765107).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26877 Genomic DNA. Translation: CAA81496.1. Sequence problems.
Z26877 Genomic DNA. Translation: CAA81497.1. Sequence problems.
Z28157 Genomic DNA. Translation: CAA81999.1. Sequence problems.
Z28158 Genomic DNA. Translation: CAA82000.1. Sequence problems.
AY558356 Genomic DNA. Translation: AAS56682.1. Sequence problems.
X63998 Genomic DNA. Translation: CAA45403.1. Sequence problems.
BK006944 Genomic DNA. Translation: DAA09007.2.
PIRiS37793.
S37794.
RefSeqiNP_012765.3. NM_001179723.2.

Genome annotation databases

EnsemblFungiiYKL157W; YKL157W; YKL157W.
GeneIDi853699.
KEGGisce:YKL157W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26877 Genomic DNA. Translation: CAA81496.1. Sequence problems.
Z26877 Genomic DNA. Translation: CAA81497.1. Sequence problems.
Z28157 Genomic DNA. Translation: CAA81999.1. Sequence problems.
Z28158 Genomic DNA. Translation: CAA82000.1. Sequence problems.
AY558356 Genomic DNA. Translation: AAS56682.1. Sequence problems.
X63998 Genomic DNA. Translation: CAA45403.1. Sequence problems.
BK006944 Genomic DNA. Translation: DAA09007.2.
PIRiS37793.
S37794.
RefSeqiNP_012765.3. NM_001179723.2.

3D structure databases

ProteinModelPortaliP32454.
SMRiP32454.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33980. 74 interactors.
DIPiDIP-4391N.
IntActiP32454. 52 interactors.
MINTiMINT-503293.

Protein family/group databases

MEROPSiM01.006.

PTM databases

iPTMnetiP32454.

Proteomic databases

MaxQBiP32454.
PRIDEiP32454.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL157W; YKL157W; YKL157W.
GeneIDi853699.
KEGGisce:YKL157W.

Organism-specific databases

EuPathDBiFungiDB:YKL157W.
SGDiS000001640. APE2.

Phylogenomic databases

GeneTreeiENSGT00760000119082.
HOGENOMiHOG000106482.
InParanoidiP32454.
KOiK13721.
OMAiHAMENDS.
OrthoDBiEOG092C19PD.

Enzyme and pathway databases

BioCyciYEAST:YKL157W-MONOMER.
ReactomeiR-SCE-1445148. Translocation of GLUT4 to the plasma membrane.
R-SCE-6798695. Neutrophil degranulation.
R-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.
R-SCE-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Miscellaneous databases

PROiP32454.

Family and domain databases

InterProiIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPE2_YEAST
AccessioniPrimary (citable) accession number: P32454
Secondary accession number(s): D6VX41, P36055
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: September 21, 2011
Last modified: November 30, 2016
This is version 161 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2910 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.