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P32454

- APE2_YEAST

UniProt

P32454 - APE2_YEAST

Protein

Aminopeptidase 2, mitochondrial

Gene

APE2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 4 (21 Sep 2011)
      Previous versions | rss
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    Functioni

    Involved in the cellular supply of leucine from externally offered leucine-containing dipeptide substrates.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei228 – 2281SubstrateBy similarity
    Metal bindingi396 – 3961Zinc; catalyticPROSITE-ProRule annotation
    Active sitei397 – 3971Proton acceptorPROSITE-ProRule annotation
    Metal bindingi400 – 4001Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi419 – 4191Zinc; catalyticPROSITE-ProRule annotation
    Sitei482 – 4821Transition state stabilizerBy similarity

    GO - Molecular functioni

    1. metalloaminopeptidase activity Source: SGD
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. peptide catabolic process Source: SGD

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:YKL157W-MONOMER.
    ReactomeiREACT_189060. Antigen processing: Ubiquitination & Proteasome degradation.

    Protein family/group databases

    MEROPSiM01.006.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aminopeptidase 2, mitochondrial (EC:3.4.11.-)
    Short name:
    AP-II
    Short name:
    Aminopeptidase II
    Alternative name(s):
    YscII
    Gene namesi
    Name:APE2
    Synonyms:LAP1
    Ordered Locus Names:YKL157W
    ORF Names:YKL158W, YKL611, YKL612
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKL157w.
    SGDiS000001640. APE2.

    Subcellular locationi

    GO - Cellular componenti

    1. cell wall-bounded periplasmic space Source: SGD
    2. extracellular region Source: SGD
    3. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion, Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5252MitochondrionSequence AnalysisAdd
    BLAST
    Chaini53 – 952900Aminopeptidase 2, mitochondrialPRO_0000095104Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi381 – 3811N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi713 – 7131N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP32454.
    PaxDbiP32454.
    PeptideAtlasiP32454.
    PRIDEiP32454.

    Expressioni

    Gene expression databases

    GenevestigatoriP32454.

    Interactioni

    Protein-protein interaction databases

    BioGridi33980. 71 interactions.
    DIPiDIP-4391N.
    IntActiP32454. 52 interactions.
    MINTiMINT-503293.
    STRINGi4932.YKL157W.

    Structurei

    3D structure databases

    ProteinModelPortaliP32454.
    SMRiP32454. Positions 102-946.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni360 – 3645Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the peptidase M1 family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0308.
    GeneTreeiENSGT00740000114965.
    HOGENOMiHOG000106482.
    KOiK13721.
    OMAiRTVQQCC.
    OrthoDBiEOG75TMMT.

    Family and domain databases

    InterProiIPR024571. ERAP1-like_C_dom.
    IPR001930. Peptidase_M1.
    IPR014782. Peptidase_M1_N.
    [Graphical view]
    PANTHERiPTHR11533. PTHR11533. 1 hit.
    PfamiPF11838. ERAP1_C. 1 hit.
    PF01433. Peptidase_M1. 1 hit.
    [Graphical view]
    PRINTSiPR00756. ALADIPTASE.
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32454-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPIVRWLLLK SAVRGSSLIG KAHPCLRSIA AHPRYLSNVY SPPAGVSRSL    50
    RINVMWKQSK LTPPRFVKIM NRRPLFTETS HACAKCQKTS QLLNKTPNRE 100
    ILPDNVVPLH YDLTVEPDFK TFKFEGSVKI ELKINNPAID TVTLNTVDTD 150
    IHSAKIGDVT SSEIISEEEQ QVTTFAFPKG TMSSFKGNAF LDIKFTGILN 200
    DNMAGFYRAK YEDKLTGETK YMATTQMEPT DARRAFPCFD EPNLKASFAI 250
    TLVSDPSLTH LSNMDVKNEY VKDGKKVTLF NTTPKMSTYL VAFIVAELKY 300
    VESKNFRIPV RVYATPGNEK HGQFAADLTA KTLAFFEKTF GIQYPLPKMD 350
    NVAVHEFSAG AMENWGLVTY RVVDLLLDKD NSTLDRIQRV AEVVQHELAH 400
    QWFGNLVTMD WWEGLWLNEG FATWMSWYSC NEFQPEWKVW EQYVTDTLQH 450
    ALSLDSLRSS HPIEVPVKKA DEINQIFDAI SYSKGASLLR MISKWLGEET 500
    FIKGVSQYLN KFKYGNAKTE DLWDALADAS GKDVRSVMNI WTKKVGFPVI 550
    SVSEDGNGKI TFRQNRYLST ADVKPDEDKT IYPVFLALKT KNGVDSSVVL 600
    SERSKTIELE DPTFFKVNSE QSGIYITSYT DERWAKLGQQ ADLLSVEDRV 650
    GLVADVKTLS ASGYTSTTNF LNLVSKWNNE KSFVVWDQII NSISSMKSTW 700
    LFEPKETQDA LDNFTKQLIS GMTHHLGWEF KSSDSFSTQR LKVTMFGAAC 750
    AARDADVEKA ALKMFTDYCS GNKEAIPALI KPIVFNTVAR VGGAENYEKV 800
    YKIYLDPISN DEKLAALRSL GRFKEPKLLE RTLGYLFDGT VLNQDIYIPM 850
    QGMRAHQEGV EALWNWVKKN WDELVKRLPP GLSMLGSVVT LGTSGFTSMQ 900
    KIDEIKKFFA TKSTKGFDQS LAQSLDTITS KAQWVNRDRD VVNKYLKENG 950
    YY 952
    Length:952
    Mass (Da):107,755
    Last modified:September 21, 2011 - v4
    Checksum:i391C068DF4A18C2D
    GO

    Sequence cautioni

    The sequence CAA81497.1 differs from that shown. Reason: Frameshift at position 935.
    The sequence CAA81999.1 differs from that shown. Reason: Frameshift at position 935.
    The sequence AAS56682.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAA45403.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAA81496.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAA81497.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAA81999.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAA82000.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti73 – 731R → A in CAA45403. (PubMed:1765107)Curated
    Sequence conflicti92 – 932LL → FI in CAA45403. (PubMed:1765107)Curated
    Sequence conflicti255 – 2551D → V in CAA45403. (PubMed:1765107)Curated
    Sequence conflicti533 – 5331D → E in CAA45403. (PubMed:1765107)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z26877 Genomic DNA. Translation: CAA81496.1. Sequence problems.
    Z26877 Genomic DNA. Translation: CAA81497.1. Sequence problems.
    Z28157 Genomic DNA. Translation: CAA81999.1. Sequence problems.
    Z28158 Genomic DNA. Translation: CAA82000.1. Sequence problems.
    AY558356 Genomic DNA. Translation: AAS56682.1. Sequence problems.
    X63998 Genomic DNA. Translation: CAA45403.1. Sequence problems.
    BK006944 Genomic DNA. Translation: DAA09007.2.
    PIRiS37793.
    S37794.
    RefSeqiNP_012765.3. NM_001179723.2.

    Genome annotation databases

    EnsemblFungiiYKL157W; YKL157W; YKL157W.
    GeneIDi853699.
    KEGGisce:YKL157W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z26877 Genomic DNA. Translation: CAA81496.1 . Sequence problems.
    Z26877 Genomic DNA. Translation: CAA81497.1 . Sequence problems.
    Z28157 Genomic DNA. Translation: CAA81999.1 . Sequence problems.
    Z28158 Genomic DNA. Translation: CAA82000.1 . Sequence problems.
    AY558356 Genomic DNA. Translation: AAS56682.1 . Sequence problems.
    X63998 Genomic DNA. Translation: CAA45403.1 . Sequence problems.
    BK006944 Genomic DNA. Translation: DAA09007.2 .
    PIRi S37793.
    S37794.
    RefSeqi NP_012765.3. NM_001179723.2.

    3D structure databases

    ProteinModelPortali P32454.
    SMRi P32454. Positions 102-946.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33980. 71 interactions.
    DIPi DIP-4391N.
    IntActi P32454. 52 interactions.
    MINTi MINT-503293.
    STRINGi 4932.YKL157W.

    Protein family/group databases

    MEROPSi M01.006.

    Proteomic databases

    MaxQBi P32454.
    PaxDbi P32454.
    PeptideAtlasi P32454.
    PRIDEi P32454.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL157W ; YKL157W ; YKL157W .
    GeneIDi 853699.
    KEGGi sce:YKL157W.

    Organism-specific databases

    CYGDi YKL157w.
    SGDi S000001640. APE2.

    Phylogenomic databases

    eggNOGi COG0308.
    GeneTreei ENSGT00740000114965.
    HOGENOMi HOG000106482.
    KOi K13721.
    OMAi RTVQQCC.
    OrthoDBi EOG75TMMT.

    Enzyme and pathway databases

    BioCyci YEAST:YKL157W-MONOMER.
    Reactomei REACT_189060. Antigen processing: Ubiquitination & Proteasome degradation.

    Miscellaneous databases

    NextBioi 974687.

    Gene expression databases

    Genevestigatori P32454.

    Family and domain databases

    InterProi IPR024571. ERAP1-like_C_dom.
    IPR001930. Peptidase_M1.
    IPR014782. Peptidase_M1_N.
    [Graphical view ]
    PANTHERi PTHR11533. PTHR11533. 1 hit.
    Pfami PF11838. ERAP1_C. 1 hit.
    PF01433. Peptidase_M1. 1 hit.
    [Graphical view ]
    PRINTSi PR00756. ALADIPTASE.
    PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae."
      Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
      Yeast 10:S35-S40(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 935.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-93.
      Strain: ATCC 204508 / S288c.
    5. "Molecular cloning of soluble aminopeptidases from Saccharomyces cerevisiae. Sequence analysis of aminopeptidase yscII, a putative zinc-metallopeptidase."
      Garcia-Alvarez N., Cueva R., Suarez-Rendueles P.
      Eur. J. Biochem. 202:993-1002(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 35-952.
      Strain: ATCC 204510 / AB320.
    6. "External and internal forms of yeast aminopeptidase II."
      Frey J., Roehm K.-H.
      Eur. J. Biochem. 97:169-173(1979) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    7. "Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast."
      Davis C.A., Grate L., Spingola M., Ares M. Jr.
      Nucleic Acids Res. 28:1700-1706(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF INTRON.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    11. "The yeast mitochondrial proteome, a study of fermentative and respiratory growth."
      Ohlmeier S., Kastaniotis A.J., Hiltunen J.K., Bergmann U.
      J. Biol. Chem. 279:3956-3979(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
    12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiAPE2_YEAST
    AccessioniPrimary (citable) accession number: P32454
    Secondary accession number(s): D6VX41, P36055
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: September 21, 2011
    Last modified: October 1, 2014
    This is version 139 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2910 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3