Reviewed, UniProtKB/Swiss-Prot P32452 (PHA2_YEAST)

Names and origin

Protein names

Recommended name:
    Putative prephenate dehydratase
      Short name=PDT
    EC=4.2.1.51
Alternative name(s):
    Phenylalanine-requiring protein 2

Gene names

Name:	PHA2
Ordered Locus Names:	YNL316C
ORF Names:	N0351

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Prephenate = phenylpyruvate + H2O + CO2.
Pathway	Amino-acid biosynthesis; L-phenylalanine biosynthesis; phenylpyruvate from prephenate: step 1/1.
Subcellular location	Cytoplasm. Ref.5
Miscellaneous	Present with 2020 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Contains 1 prephenate dehydratase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Phenylalanine biosynthesis
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine biosynthetic process Ref.1 Traceable author statement. Source: SGD
Cellular component	cytoplasm Ref.5 Inferred from direct assay. Source: SGD
Molecular function	prephenate dehydratase activity Ref.1 Traceable author statement. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Putative prephenate dehydratase		PRO_0000119206
Regions
	Domain	7 – 224	218	Prephenate dehydratase

Sequences

	Sequence		Length	Mass (Da)
	P32452-1 [UniParc]. Last modified October 17, 2006. Version 3. Checksum: 1917F12AE5ECC249	FASTA	334	38,225
	10 20 30 40 50 60 MASKTLRVLF LGPKGTYSHQ AALQQFQSTS DVEYLPAASI PQCFNQLEND TSIDYSVVPL 70 80 90 100 110 120 ENSTNGQVVF SYDLLRDRMI KKALSLPAPA DTNRITPDIE VIAEQYVPIT HCLISPIQLP 130 140 150 160 170 180 NGIASLGNFE EVIIHSHPQV WGQVECYLRS MAEKFPQVTF IRLDCSSTSE SVNQCIRSST 190 200 210 220 230 240 ADCDNILHLA IASETAAQLH KAYIIEHSIN DKLGNTTRFL VLKRRENAGD NEVEDTGLLR 250 260 270 280 290 300 VNLLTFTTRQ DDPGSLVDVL NILKIHSLNM CSINSRPFHL DEHDRNWRYL FFIEYYTEKN 310 320 330 TPKNKEKFYE DISDKSKQWC LWGTFPRNER YYHK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:1077-1085(1995) [PubMed: 7502583] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Characterization of ATP11 and detection of the encoded protein in mitochondria of Saccharomyces cerevisiae." Ackerman S.H., Martin J., Tzagoloff A. J. Biol. Chem. 267:7386-7394(1992) [PubMed: 1532796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-59.
[4]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed: 12748633] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z46259 Genomic DNA. Translation: CAA86380.1. Different initiation. Z71592 Genomic DNA. Translation: CAA96246.1. Different initiation. M87006 Genomic DNA. Translation: AAA34448.1. Different initiation.
PIR	S59565.
RefSeq	NP_014083.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4269N.
IntAct	P32452. 3 interactions.
STRING	P32452.
Proteomic databases
PeptideAtlas	P32452.
Genome annotation databases
Ensembl	YNL316C; YNL316C; YNL316C; Saccharomyces cerevisiae. [Genome view]
GeneID	855400.
GenomeReviews	Gene locus YNL316C in contig Y13139_GR.
KEGG	sce:YNL316C.
NMPDR	fig|4932.3.peg.5145.
Organism-specific databases
CYGD	YNL316c.
SGD	S000005260. PHA2.
Phylogenomic databases
HOGENOM	P32452.
OMA	LGNTTRF.
Enzyme and pathway databases
BRENDA	4.2.1.51. 250.
Gene expression databases
ArrayExpress	P32452.
Genevestigator	P32452.
GermOnline	YNL316C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001086. Preph_deHydtase. IPR018528. Preph_deHydtase_CS. [Graphical view]
Pfam	PF00800. PDT. 1 hit. [Graphical view]
PROSITE	PS00857. PREPHENATE_DEHYDR_1. False negative. PS00858. PREPHENATE_DEHYDR_2. False negative. PS51171. PREPHENATE_DEHYDR_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	979223.

Entry information

Entry name

PHA2_YEAST

Accession

Primary (citable) accession number: P32452

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 17, 2006
Last modified:	November 3, 2009
This is version 73 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)