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P32451 (BIOB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase, mitochondrial

EC=2.8.1.6
Gene names
Name:BIO2
Ordered Locus Names:YGR286C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length375 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subcellular location

Mitochondrion Ref.5.

Miscellaneous

Present with 504 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence caution

The sequence CAA51253.1 differs from that shown. Reason: Erroneous termination at position 48. Translated as Glu.

The sequence CAA51253.1 differs from that shown. Reason: Frameshift at positions 37, 73, 77, 129 and 134.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 1616Mitochondrion Potential
Chain17 – 375359Biotin synthase, mitochondrial HAMAP-Rule MF_01694
PRO_0000185567

Sites

Metal binding991Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1031Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1061Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1431Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1761Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2361Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding3141Iron-sulfur 2 (2Fe-2S) By similarity

Experimental info

Sequence conflict348 – 3492MC → IY in CAA51253. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P32451 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: BE44E0B34AA3942A

FASTA37541,884
        10         20         30         40         50         60 
MMSTIYRHLS TARPALTKYA TNAAVKSTTA SSEASTLGAL QYALSLDEPS HSWTKSQLKE 

        70         80         90        100        110        120 
IYHTPLLELT HAAQLQHRKW HDPTKVQLCT LMNIKSGGCS EDCKYCAQSS RNDTGLKAEK 

       130        140        150        160        170        180 
MVKVDEVIKE AEEAKRNGST RFCLGAAWRD MKGRKSAMKR IQEMVTKVND MGLETCVTLG 

       190        200        210        220        230        240 
MVDQDQAKQL KDAGLTAYNH NIDTSREHYS KVITTRTYDD RLQTIKNVQE SGIKACTGGI 

       250        260        270        280        290        300 
LGLGESEDDH IGFIYTLSNM SPHPESLPIN RLVAIKGTPM AEELADPKSK KLQFDEILRT 

       310        320        330        340        350        360 
IATARIVMPK AIIRLAAGRY TMKETEQFVC FMAGCNSIFT GKKMLTTMCN GWDEDKAMLA 

       370 
KWGLQPMEAF KYDRS 

« Hide

References

« Hide 'large scale' references
[1]"The gene for biotin synthase from Saccharomyces cerevisiae: cloning, sequencing, and complementation of Escherichia coli strains lacking biotin synthase."
Zhang S., Sanyal I., Bulboaca G.H., Rich A., Flint D.H.
Arch. Biochem. Biophys. 309:29-35(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 20B-12.
[2]"Sequence analysis of a near-subtelomeric 35.4 kb DNA segment on the right arm of chromosome VII from Saccharomyces cerevisiae carrying the MAL1 locus reveals 15 complete open reading frames, including ZUO1, BGL2 and BIO2 genes and an ABC transporter gene."
Volckaert G., Voet M., Robben J.
Yeast 13:251-259(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X72701 Genomic DNA. Translation: CAA51253.1. Sequence problems.
Z73071 Genomic DNA. Translation: CAA97318.1.
BK006941 Genomic DNA. Translation: DAA08374.1.
PIRS64621.
RefSeqNP_011802.1. NM_001181415.1.

3D structure databases

ProteinModelPortalP32451.
SMRP32451. Positions 53-368.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33536. 35 interactions.
IntActP32451. 1 interaction.
MINTMINT-2785666.
STRING4932.YGR286C.

Proteomic databases

MaxQBP32451.
PaxDbP32451.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR286C; YGR286C; YGR286C.
GeneID853203.
KEGGsce:YGR286C.

Organism-specific databases

CYGDYGR286c.
SGDS000003518. BIO2.

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMANGWDEDK.
OrthoDBEOG7V76GZ.

Enzyme and pathway databases

BioCycYEAST:YGR286C-MONOMER.
UniPathwayUPA00078; UER00162.

Gene expression databases

GenevestigatorP32451.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Other

NextBio973380.
PROP32451.

Entry information

Entry nameBIOB_YEAST
AccessionPrimary (citable) accession number: P32451
Secondary accession number(s): D6VV63
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways