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P32451

- BIOB_YEAST

UniProt

P32451 - BIOB_YEAST

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Protein

Biotin synthase, mitochondrial

Gene

BIO2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine (By similarity).By similarity
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi99 – 991Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
Metal bindingi103 – 1031Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
Metal bindingi106 – 1061Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
Metal bindingi143 – 1431Iron-sulfur 2 (2Fe-2S)By similarity
Metal bindingi176 – 1761Iron-sulfur 2 (2Fe-2S)By similarity
Metal bindingi236 – 2361Iron-sulfur 2 (2Fe-2S)By similarity
Metal bindingi314 – 3141Iron-sulfur 2 (2Fe-2S)By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: SGD
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. biotin biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:YGR286C-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase, mitochondrial (EC:2.8.1.6)
Gene namesi
Name:BIO2
Ordered Locus Names:YGR286C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR286c.
SGDiS000003518. BIO2.

Subcellular locationi

Mitochondrion 1 Publication

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1616MitochondrionSequence AnalysisAdd
BLAST
Chaini17 – 375359Biotin synthase, mitochondrialPRO_0000185567Add
BLAST

Proteomic databases

MaxQBiP32451.
PaxDbiP32451.

Expressioni

Gene expression databases

GenevestigatoriP32451.

Interactioni

Protein-protein interaction databases

BioGridi33536. 35 interactions.
IntActiP32451. 1 interaction.
MINTiMINT-2785666.
STRINGi4932.YGR286C.

Structurei

3D structure databases

ProteinModelPortaliP32451.
SMRiP32451. Positions 53-368.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
InParanoidiP32451.
KOiK01012.
OMAiNGWDEDK.
OrthoDBiEOG7V76GZ.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32451-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMSTIYRHLS TARPALTKYA TNAAVKSTTA SSEASTLGAL QYALSLDEPS
60 70 80 90 100
HSWTKSQLKE IYHTPLLELT HAAQLQHRKW HDPTKVQLCT LMNIKSGGCS
110 120 130 140 150
EDCKYCAQSS RNDTGLKAEK MVKVDEVIKE AEEAKRNGST RFCLGAAWRD
160 170 180 190 200
MKGRKSAMKR IQEMVTKVND MGLETCVTLG MVDQDQAKQL KDAGLTAYNH
210 220 230 240 250
NIDTSREHYS KVITTRTYDD RLQTIKNVQE SGIKACTGGI LGLGESEDDH
260 270 280 290 300
IGFIYTLSNM SPHPESLPIN RLVAIKGTPM AEELADPKSK KLQFDEILRT
310 320 330 340 350
IATARIVMPK AIIRLAAGRY TMKETEQFVC FMAGCNSIFT GKKMLTTMCN
360 370
GWDEDKAMLA KWGLQPMEAF KYDRS
Length:375
Mass (Da):41,884
Last modified:October 1, 1996 - v2
Checksum:iBE44E0B34AA3942A
GO

Sequence cautioni

The sequence CAA51253.1 differs from that shown. Reason: Frameshift at positions 37, 73, 77, 129 and 134.
The sequence CAA51253.1 differs from that shown. Reason: Erroneous termination at position 48. Translated as Glu.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti348 – 3492MC → IY in CAA51253. (PubMed:8117110)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X72701 Genomic DNA. Translation: CAA51253.1. Sequence problems.
Z73071 Genomic DNA. Translation: CAA97318.1.
BK006941 Genomic DNA. Translation: DAA08374.1.
PIRiS64621.
RefSeqiNP_011802.1. NM_001181415.1.

Genome annotation databases

EnsemblFungiiYGR286C; YGR286C; YGR286C.
GeneIDi853203.
KEGGisce:YGR286C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X72701 Genomic DNA. Translation: CAA51253.1 . Sequence problems.
Z73071 Genomic DNA. Translation: CAA97318.1 .
BK006941 Genomic DNA. Translation: DAA08374.1 .
PIRi S64621.
RefSeqi NP_011802.1. NM_001181415.1.

3D structure databases

ProteinModelPortali P32451.
SMRi P32451. Positions 53-368.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33536. 35 interactions.
IntActi P32451. 1 interaction.
MINTi MINT-2785666.
STRINGi 4932.YGR286C.

Proteomic databases

MaxQBi P32451.
PaxDbi P32451.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR286C ; YGR286C ; YGR286C .
GeneIDi 853203.
KEGGi sce:YGR286C.

Organism-specific databases

CYGDi YGR286c.
SGDi S000003518. BIO2.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
InParanoidi P32451.
KOi K01012.
OMAi NGWDEDK.
OrthoDBi EOG7V76GZ.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci YEAST:YGR286C-MONOMER.

Miscellaneous databases

NextBioi 973380.
PROi P32451.

Gene expression databases

Genevestigatori P32451.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The gene for biotin synthase from Saccharomyces cerevisiae: cloning, sequencing, and complementation of Escherichia coli strains lacking biotin synthase."
    Zhang S., Sanyal I., Bulboaca G.H., Rich A., Flint D.H.
    Arch. Biochem. Biophys. 309:29-35(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 20B-12.
  2. "Sequence analysis of a near-subtelomeric 35.4 kb DNA segment on the right arm of chromosome VII from Saccharomyces cerevisiae carrying the MAL1 locus reveals 15 complete open reading frames, including ZUO1, BGL2 and BIO2 genes and an ABC transporter gene."
    Volckaert G., Voet M., Robben J.
    Yeast 13:251-259(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBIOB_YEAST
AccessioniPrimary (citable) accession number: P32451
Secondary accession number(s): D6VV63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 504 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3