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P32451

- BIOB_YEAST

UniProt

P32451 - BIOB_YEAST

Protein

Biotin synthase, mitochondrial

Gene

BIO2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.By similarity
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi99 – 991Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi103 – 1031Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi106 – 1061Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi143 – 1431Iron-sulfur 2 (2Fe-2S)By similarity
    Metal bindingi176 – 1761Iron-sulfur 2 (2Fe-2S)By similarity
    Metal bindingi236 – 2361Iron-sulfur 2 (2Fe-2S)By similarity
    Metal bindingi314 – 3141Iron-sulfur 2 (2Fe-2S)By similarity

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: SGD
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. biotin biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciYEAST:YGR286C-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthase, mitochondrial (EC:2.8.1.6)
    Gene namesi
    Name:BIO2
    Ordered Locus Names:YGR286C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR286c.
    SGDiS000003518. BIO2.

    Subcellular locationi

    Mitochondrion 1 Publication

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 1616MitochondrionSequence AnalysisAdd
    BLAST
    Chaini17 – 375359Biotin synthase, mitochondrialPRO_0000185567Add
    BLAST

    Proteomic databases

    MaxQBiP32451.
    PaxDbiP32451.

    Expressioni

    Gene expression databases

    GenevestigatoriP32451.

    Interactioni

    Protein-protein interaction databases

    BioGridi33536. 35 interactions.
    IntActiP32451. 1 interaction.
    MINTiMINT-2785666.
    STRINGi4932.YGR286C.

    Structurei

    3D structure databases

    ProteinModelPortaliP32451.
    SMRiP32451. Positions 53-368.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiNGWDEDK.
    OrthoDBiEOG7V76GZ.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32451-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMSTIYRHLS TARPALTKYA TNAAVKSTTA SSEASTLGAL QYALSLDEPS    50
    HSWTKSQLKE IYHTPLLELT HAAQLQHRKW HDPTKVQLCT LMNIKSGGCS 100
    EDCKYCAQSS RNDTGLKAEK MVKVDEVIKE AEEAKRNGST RFCLGAAWRD 150
    MKGRKSAMKR IQEMVTKVND MGLETCVTLG MVDQDQAKQL KDAGLTAYNH 200
    NIDTSREHYS KVITTRTYDD RLQTIKNVQE SGIKACTGGI LGLGESEDDH 250
    IGFIYTLSNM SPHPESLPIN RLVAIKGTPM AEELADPKSK KLQFDEILRT 300
    IATARIVMPK AIIRLAAGRY TMKETEQFVC FMAGCNSIFT GKKMLTTMCN 350
    GWDEDKAMLA KWGLQPMEAF KYDRS 375
    Length:375
    Mass (Da):41,884
    Last modified:October 1, 1996 - v2
    Checksum:iBE44E0B34AA3942A
    GO

    Sequence cautioni

    The sequence CAA51253.1 differs from that shown. Reason: Frameshift at positions 37, 73, 77, 129 and 134.
    The sequence CAA51253.1 differs from that shown. Reason: Erroneous termination at position 48. Translated as Glu.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti348 – 3492MC → IY in CAA51253. (PubMed:8117110)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72701 Genomic DNA. Translation: CAA51253.1. Sequence problems.
    Z73071 Genomic DNA. Translation: CAA97318.1.
    BK006941 Genomic DNA. Translation: DAA08374.1.
    PIRiS64621.
    RefSeqiNP_011802.1. NM_001181415.1.

    Genome annotation databases

    EnsemblFungiiYGR286C; YGR286C; YGR286C.
    GeneIDi853203.
    KEGGisce:YGR286C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72701 Genomic DNA. Translation: CAA51253.1 . Sequence problems.
    Z73071 Genomic DNA. Translation: CAA97318.1 .
    BK006941 Genomic DNA. Translation: DAA08374.1 .
    PIRi S64621.
    RefSeqi NP_011802.1. NM_001181415.1.

    3D structure databases

    ProteinModelPortali P32451.
    SMRi P32451. Positions 53-368.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33536. 35 interactions.
    IntActi P32451. 1 interaction.
    MINTi MINT-2785666.
    STRINGi 4932.YGR286C.

    Proteomic databases

    MaxQBi P32451.
    PaxDbi P32451.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR286C ; YGR286C ; YGR286C .
    GeneIDi 853203.
    KEGGi sce:YGR286C.

    Organism-specific databases

    CYGDi YGR286c.
    SGDi S000003518. BIO2.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi NGWDEDK.
    OrthoDBi EOG7V76GZ.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci YEAST:YGR286C-MONOMER.

    Miscellaneous databases

    NextBioi 973380.
    PROi P32451.

    Gene expression databases

    Genevestigatori P32451.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The gene for biotin synthase from Saccharomyces cerevisiae: cloning, sequencing, and complementation of Escherichia coli strains lacking biotin synthase."
      Zhang S., Sanyal I., Bulboaca G.H., Rich A., Flint D.H.
      Arch. Biochem. Biophys. 309:29-35(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 20B-12.
    2. "Sequence analysis of a near-subtelomeric 35.4 kb DNA segment on the right arm of chromosome VII from Saccharomyces cerevisiae carrying the MAL1 locus reveals 15 complete open reading frames, including ZUO1, BGL2 and BIO2 genes and an ABC transporter gene."
      Volckaert G., Voet M., Robben J.
      Yeast 13:251-259(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBIOB_YEAST
    AccessioniPrimary (citable) accession number: P32451
    Secondary accession number(s): D6VV63
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 504 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3