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Protein

Biotin synthase, mitochondrial

Gene

BIO2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 504 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.By similarity

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Dethiobiotin synthetase (BIO4)
  2. Biotin synthase, mitochondrial (BIO2)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi99Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi103Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi106Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi143Iron-sulfur 2 (2Fe-2S)By similarity1
Metal bindingi176Iron-sulfur 2 (2Fe-2S)By similarity1
Metal bindingi236Iron-sulfur 2 (2Fe-2S)By similarity1
Metal bindingi314Iron-sulfur 2 (2Fe-2S)By similarity1

GO - Molecular functioni

  • 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  • 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  • biotin synthase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • biotin biosynthetic process Source: SGD

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:YGR286C-MONOMER
UniPathwayiUPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase, mitochondrial (EC:2.8.1.6)
Gene namesi
Name:BIO2
Ordered Locus Names:YGR286C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR286C
SGDiS000003518 BIO2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 16MitochondrionSequence analysisAdd BLAST16
ChainiPRO_000018556717 – 375Biotin synthase, mitochondrialAdd BLAST359

Proteomic databases

MaxQBiP32451
PaxDbiP32451
PRIDEiP32451

Interactioni

Protein-protein interaction databases

BioGridi33536, 91 interactors
IntActiP32451, 1 interactor
MINTiP32451
STRINGi4932.YGR286C

Structurei

3D structure databases

ProteinModelPortaliP32451
SMRiP32451
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000239957
InParanoidiP32451
KOiK01012
OMAiADRFCMG
OrthoDBiEOG092C24WQ

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDF00272 biotin_synthase, 1 hit
SFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32451-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSTIYRHLS TARPALTKYA TNAAVKSTTA SSEASTLGAL QYALSLDEPS
60 70 80 90 100
HSWTKSQLKE IYHTPLLELT HAAQLQHRKW HDPTKVQLCT LMNIKSGGCS
110 120 130 140 150
EDCKYCAQSS RNDTGLKAEK MVKVDEVIKE AEEAKRNGST RFCLGAAWRD
160 170 180 190 200
MKGRKSAMKR IQEMVTKVND MGLETCVTLG MVDQDQAKQL KDAGLTAYNH
210 220 230 240 250
NIDTSREHYS KVITTRTYDD RLQTIKNVQE SGIKACTGGI LGLGESEDDH
260 270 280 290 300
IGFIYTLSNM SPHPESLPIN RLVAIKGTPM AEELADPKSK KLQFDEILRT
310 320 330 340 350
IATARIVMPK AIIRLAAGRY TMKETEQFVC FMAGCNSIFT GKKMLTTMCN
360 370
GWDEDKAMLA KWGLQPMEAF KYDRS
Length:375
Mass (Da):41,884
Last modified:October 1, 1996 - v2
Checksum:iBE44E0B34AA3942A
GO

Sequence cautioni

The sequence CAA51253 differs from that shown. Reason: Frameshift at positions 37, 73, 77, 129 and 134.Curated
The sequence CAA51253 differs from that shown. Reason: Erroneous termination at position 48. Translated as Glu.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti348 – 349MC → IY in CAA51253 (PubMed:8117110).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72701 Genomic DNA Translation: CAA51253.1 Sequence problems.
Z73071 Genomic DNA Translation: CAA97318.1
BK006941 Genomic DNA Translation: DAA08374.1
PIRiS64621
RefSeqiNP_011802.1, NM_001181415.1

Genome annotation databases

EnsemblFungiiYGR286C; YGR286C; YGR286C
GeneIDi853203
KEGGisce:YGR286C

Similar proteinsi

Entry informationi

Entry nameiBIOB_YEAST
AccessioniPrimary (citable) accession number: P32451
Secondary accession number(s): D6VV63
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

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