P32451 (BIOB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Biotin synthase, mitochondrial
EC=2.8.1.6

Gene names

Name:	BIO2
Ordered Locus Names:	YGR286C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Dethiobiotin + sulfur + 2 S-adenosyl-L-methionine = biotin + 2 L-methionine + 2 5'-deoxyadenosine.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.
Subcellular location	Mitochondrion Ref.5.
Miscellaneous	Present with 504 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the radical SAM superfamily. Biotin synthase family.
Sequence caution	The sequence CAA51253.1 differs from that shown. Reason: Erroneous termination at position 48. Translated as Glu. The sequence CAA51253.1 differs from that shown. Reason: Frameshift at positions 37, 73, 77, 129 and 134.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	biotin biosynthetic process Inferred from mutant phenotype PubMed 10525840. Source: SGD
Cellular_component	mitochondrion Inferred from direct assay Ref.5. Source: SGD
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW biotin synthase activity Inferred from mutant phenotype PubMed 10525840. Source: SGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 16	16	Mitochondrion Potential
	Chain	17 – 375	359	Biotin synthase, mitochondrial		PRO_0000185567
Sites
	Metal binding	99	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	103	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	106	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	143	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	176	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	236	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	314	1	Iron-sulfur 2 (2Fe-2S) By similarity
Experimental info
	Sequence conflict	348 – 349	2	MC → IY in CAA51253. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32451 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: BE44E0B34AA3942A	FASTA	375	41,884
	10 20 30 40 50 60 MMSTIYRHLS TARPALTKYA TNAAVKSTTA SSEASTLGAL QYALSLDEPS HSWTKSQLKE 70 80 90 100 110 120 IYHTPLLELT HAAQLQHRKW HDPTKVQLCT LMNIKSGGCS EDCKYCAQSS RNDTGLKAEK 130 140 150 160 170 180 MVKVDEVIKE AEEAKRNGST RFCLGAAWRD MKGRKSAMKR IQEMVTKVND MGLETCVTLG 190 200 210 220 230 240 MVDQDQAKQL KDAGLTAYNH NIDTSREHYS KVITTRTYDD RLQTIKNVQE SGIKACTGGI 250 260 270 280 290 300 LGLGESEDDH IGFIYTLSNM SPHPESLPIN RLVAIKGTPM AEELADPKSK KLQFDEILRT 310 320 330 340 350 360 IATARIVMPK AIIRLAAGRY TMKETEQFVC FMAGCNSIFT GKKMLTTMCN GWDEDKAMLA 370 KWGLQPMEAF KYDRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The gene for biotin synthase from Saccharomyces cerevisiae: cloning, sequencing, and complementation of Escherichia coli strains lacking biotin synthase." Zhang S., Sanyal I., Bulboaca G.H., Rich A., Flint D.H. Arch. Biochem. Biophys. 309:29-35(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 20B-12.
[2]	"Sequence analysis of a near-subtelomeric 35.4 kb DNA segment on the right arm of chromosome VII from Saccharomyces cerevisiae carrying the MAL1 locus reveals 15 complete open reading frames, including ZUO1, BGL2 and BIO2 genes and an ABC transporter gene." Volckaert G., Voet M., Robben J. Yeast 13:251-259(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X72701 Genomic DNA. Translation: CAA51253.1. Sequence problems. Z73071 Genomic DNA. Translation: CAA97318.1. BK006941 Genomic DNA. Translation: DAA08374.1.
PIR	S64621.
RefSeq	NP_011802.1. NM_001181415.1.
3D structure databases
ProteinModelPortal	P32451.
SMR	P32451. Positions 53-368.
ModBase	Search...
Protein-protein interaction databases
IntAct	P32451. 1 interaction.
MINT	MINT-2785666.
STRING	4932.YGR286C.
Proteomic databases
PaxDb	P32451.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR286C; YGR286C; YGR286C.
GeneID	853203.
KEGG	sce:YGR286C.
Organism-specific databases
CYGD	YGR286c.
SGD	S000003518. BIO2.
Phylogenomic databases
eggNOG	COG0502.
HOGENOM	HOG000239957.
KO	K01012.
OMA	RIMMPAS.
OrthoDB	EOG4BS0VB.
Enzyme and pathway databases
UniPathway	UPA00078; UER00162.
Gene expression databases
Genevestigator	P32451.
GermOnline	YGR286C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR010722. Biotin/thiamin_synth-assoc. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM. [Graphical view]
Pfam	PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF001619. Biotin_synth. 1 hit.
SMART	SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00433. bioB. 1 hit.
ProtoNet	Search...
Other
NextBio	973380.

Entry information

Entry name

BIOB_YEAST

Accession

Primary (citable) accession number: P32451
Secondary accession number(s): D6VV63

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 119 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program