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P32449

- AROG_YEAST

UniProt

P32449 - AROG_YEAST

Protein

Phospho-2-dehydro-3-deoxyheptonate aldolase, tyrosine-inhibited

Gene

ARO4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 2 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).

    Catalytic activityi

    Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.

    Enzyme regulationi

    Inhibited by tyrosine.

    Pathwayi

    GO - Molecular functioni

    1. 3-deoxy-7-phosphoheptulonate synthase activity Source: SGD

    GO - Biological processi

    1. aromatic amino acid family biosynthetic process Source: UniProtKB-KW
    2. chorismate biosynthetic process Source: SGD
    3. response to stress Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Stress response

    Enzyme and pathway databases

    BioCyciYEAST:YBR249C-MONOMER.
    UniPathwayiUPA00053; UER00084.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phospho-2-dehydro-3-deoxyheptonate aldolase, tyrosine-inhibited (EC:2.5.1.54)
    Alternative name(s):
    3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
    DAHP synthase
    Phospho-2-keto-3-deoxyheptonate aldolase
    Gene namesi
    Name:ARO4
    Ordered Locus Names:YBR249C
    ORF Names:YBR1701
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR249c.
    SGDiS000000453. ARO4.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 370369Phospho-2-dehydro-3-deoxyheptonate aldolase, tyrosine-inhibitedPRO_0000140851Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP32449.
    PaxDbiP32449.
    PeptideAtlasiP32449.
    PRIDEiP32449.

    Expressioni

    Inductioni

    By amino acid starvation.

    Gene expression databases

    GenevestigatoriP32449.

    Interactioni

    Protein-protein interaction databases

    BioGridi32944. 27 interactions.
    DIPiDIP-4175N.
    IntActiP32449. 6 interactions.
    MINTiMINT-534785.
    STRINGi4932.YBR249C.

    Structurei

    Secondary structure

    1
    370
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi25 – 295
    Helixi34 – 407
    Helixi45 – 6218
    Beta strandi68 – 747
    Helixi81 – 9818
    Turni99 – 1013
    Beta strandi102 – 1076
    Beta strandi115 – 1184
    Turni122 – 1243
    Beta strandi128 – 1303
    Helixi134 – 14916
    Turni150 – 1523
    Beta strandi155 – 1584
    Beta strandi161 – 1633
    Helixi165 – 1684
    Helixi169 – 1713
    Beta strandi173 – 1775
    Turni179 – 1835
    Helixi185 – 1928
    Beta strandi198 – 2014
    Helixi209 – 21810
    Beta strandi223 – 2275
    Beta strandi231 – 2388
    Beta strandi244 – 2485
    Helixi259 – 26810
    Beta strandi276 – 2816
    Helixi282 – 2854
    Helixi289 – 2913
    Helixi292 – 30413
    Beta strandi309 – 3179
    Beta strandi319 – 3235
    Helixi330 – 3334
    Beta strandi339 – 3424
    Helixi347 – 36721

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1HFBX-ray1.90A/B/C/D/E/F/G/H1-370[»]
    1OABX-ray1.90A/B1-370[»]
    1OF6X-ray2.10A/B/C/D/E/F/G/H1-370[»]
    1OF8X-ray1.50A/B1-370[»]
    1OFAX-ray2.01A/B1-370[»]
    1OFBX-ray2.00A/B1-370[»]
    1OFOX-ray1.85A/B1-370[»]
    1OFPX-ray2.10A/B/C/D1-370[»]
    1OFQX-ray2.70A/B/C/D1-370[»]
    1OFRX-ray2.70A/B/C/D/E/F/G/H1-370[»]
    1OG0X-ray2.70A/B/C/D/E/F/G/H1-370[»]
    ProteinModelPortaliP32449.
    SMRiP32449. Positions 23-370.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP32449.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the class-I DAHP synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG0722.
    GeneTreeiENSGT00390000001891.
    HOGENOMiHOG000220501.
    KOiK01626.
    OMAiQYMSELV.
    OrthoDBiEOG76DV30.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR006218. DAHP1/KDSA.
    IPR006219. DHAP_synth_1.
    [Graphical view]
    PANTHERiPTHR21225. PTHR21225. 1 hit.
    PfamiPF00793. DAHP_synth_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001361. DAHP_synthase. 1 hit.
    TIGRFAMsiTIGR00034. aroFGH. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32449-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSESPMFAAN GMPKVNQGAE EDVRILGYDP LASPALLQVQ IPATPTSLET    50
    AKRGRREAID IITGKDDRVL VIVGPCSIHD LEAAQEYALR LKKLSDELKG 100
    DLSIIMRAYL EKPRTTVGWK GLINDPDVNN TFNINKGLQS ARQLFVNLTN 150
    IGLPIGSEML DTISPQYLAD LVSFGAIGAR TTESQLHREL ASGLSFPVGF 200
    KNGTDGTLNV AVDACQAAAH SHHFMGVTKH GVAAITTTKG NEHCFVILRG 250
    GKKGTNYDAK SVAEAKAQLP AGSNGLMIDY SHGNSNKDFR NQPKVNDVVC 300
    EQIANGENAI TGVMIESNIN EGNQGIPAEG KAGLKYGVSI TDACIGWETT 350
    EDVLRKLAAA VRQRREVNKK 370
    Length:370
    Mass (Da):39,749
    Last modified:February 1, 1994 - v2
    Checksum:i594ED48F24175979
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61107 Genomic DNA. Translation: CAA43419.1.
    L20296 Genomic DNA. Translation: AAA65607.1.
    Z36118 Genomic DNA. Translation: CAA85212.1.
    BK006936 Genomic DNA. Translation: DAA07365.1.
    PIRiS38185.
    RefSeqiNP_009808.1. NM_001178597.1.

    Genome annotation databases

    EnsemblFungiiYBR249C; YBR249C; YBR249C.
    GeneIDi852551.
    KEGGisce:YBR249C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61107 Genomic DNA. Translation: CAA43419.1 .
    L20296 Genomic DNA. Translation: AAA65607.1 .
    Z36118 Genomic DNA. Translation: CAA85212.1 .
    BK006936 Genomic DNA. Translation: DAA07365.1 .
    PIRi S38185.
    RefSeqi NP_009808.1. NM_001178597.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1HFB X-ray 1.90 A/B/C/D/E/F/G/H 1-370 [» ]
    1OAB X-ray 1.90 A/B 1-370 [» ]
    1OF6 X-ray 2.10 A/B/C/D/E/F/G/H 1-370 [» ]
    1OF8 X-ray 1.50 A/B 1-370 [» ]
    1OFA X-ray 2.01 A/B 1-370 [» ]
    1OFB X-ray 2.00 A/B 1-370 [» ]
    1OFO X-ray 1.85 A/B 1-370 [» ]
    1OFP X-ray 2.10 A/B/C/D 1-370 [» ]
    1OFQ X-ray 2.70 A/B/C/D 1-370 [» ]
    1OFR X-ray 2.70 A/B/C/D/E/F/G/H 1-370 [» ]
    1OG0 X-ray 2.70 A/B/C/D/E/F/G/H 1-370 [» ]
    ProteinModelPortali P32449.
    SMRi P32449. Positions 23-370.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32944. 27 interactions.
    DIPi DIP-4175N.
    IntActi P32449. 6 interactions.
    MINTi MINT-534785.
    STRINGi 4932.YBR249C.

    Proteomic databases

    MaxQBi P32449.
    PaxDbi P32449.
    PeptideAtlasi P32449.
    PRIDEi P32449.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR249C ; YBR249C ; YBR249C .
    GeneIDi 852551.
    KEGGi sce:YBR249C.

    Organism-specific databases

    CYGDi YBR249c.
    SGDi S000000453. ARO4.

    Phylogenomic databases

    eggNOGi COG0722.
    GeneTreei ENSGT00390000001891.
    HOGENOMi HOG000220501.
    KOi K01626.
    OMAi QYMSELV.
    OrthoDBi EOG76DV30.

    Enzyme and pathway databases

    UniPathwayi UPA00053 ; UER00084 .
    BioCyci YEAST:YBR249C-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P32449.
    NextBioi 971640.

    Gene expression databases

    Genevestigatori P32449.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR006218. DAHP1/KDSA.
    IPR006219. DHAP_synth_1.
    [Graphical view ]
    PANTHERi PTHR21225. PTHR21225. 1 hit.
    Pfami PF00793. DAHP_synth_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001361. DAHP_synthase. 1 hit.
    TIGRFAMsi TIGR00034. aroFGH. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, primary structure and regulation of the ARO4 gene, encoding the tyrosine-inhibited 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Saccharomyces cerevisiae."
      Kuenzler M., Paravicini G., Egli C., Irniger S., Braus G.H.
      Gene 113:67-74(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Kuenzler M.
      Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 205-207.
    3. "The complete sequence of a 6794 bp segment located on the right arm of chromosome II of Saccharomyces cerevisiae. Finding of a putative dUTPase in a yeast."
      Doignon F., Biteau N., Aigle M., Crouzet M.
      Yeast 9:1131-1137(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "Cloning, primary structure, and regulation of the HIS7 gene encoding a bifunctional glutamine amidotransferase: cyclase from Saccharomyces cerevisiae."
      Kuenzler M., Balmelli T., Egli C.M., Paravicini G., Braus G.H.
      J. Bacteriol. 175:5548-5558(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 352-370.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    10. "Evolution of feedback-inhibited beta/alpha barrel isoenzymes by gene duplication and a single mutation."
      Hartmann M., Schneider T.R., Pfeil A., Heinrich G., Lipscomb W.N., Braus G.H.
      Proc. Natl. Acad. Sci. U.S.A. 100:862-867(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 23-369.

    Entry informationi

    Entry nameiAROG_YEAST
    AccessioniPrimary (citable) accession number: P32449
    Secondary accession number(s): D6VQP5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 136 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 26300 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3