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P32449 (AROG_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phospho-2-dehydro-3-deoxyheptonate aldolase, tyrosine-inhibited

EC=2.5.1.54
Alternative name(s):
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
DAHP synthase
Phospho-2-keto-3-deoxyheptonate aldolase
Gene names
Name:ARO4
Ordered Locus Names:YBR249C
ORF Names:YBR1701
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).

Catalytic activity

Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.

Enzyme regulation

Inhibited by tyrosine.

Pathway

Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.

Induction

By amino acid starvation.

Miscellaneous

Present with 26300 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the class-I DAHP synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.9
Chain2 – 370369Phospho-2-dehydro-3-deoxyheptonate aldolase, tyrosine-inhibited
PRO_0000140851

Amino acid modifications

Modified residue21N-acetylserine Ref.9

Secondary structure

............................................................... 370
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P32449 [UniParc].

Last modified February 1, 1994. Version 2.
Checksum: 594ED48F24175979

FASTA37039,749
        10         20         30         40         50         60 
MSESPMFAAN GMPKVNQGAE EDVRILGYDP LASPALLQVQ IPATPTSLET AKRGRREAID 

        70         80         90        100        110        120 
IITGKDDRVL VIVGPCSIHD LEAAQEYALR LKKLSDELKG DLSIIMRAYL EKPRTTVGWK 

       130        140        150        160        170        180 
GLINDPDVNN TFNINKGLQS ARQLFVNLTN IGLPIGSEML DTISPQYLAD LVSFGAIGAR 

       190        200        210        220        230        240 
TTESQLHREL ASGLSFPVGF KNGTDGTLNV AVDACQAAAH SHHFMGVTKH GVAAITTTKG 

       250        260        270        280        290        300 
NEHCFVILRG GKKGTNYDAK SVAEAKAQLP AGSNGLMIDY SHGNSNKDFR NQPKVNDVVC 

       310        320        330        340        350        360 
EQIANGENAI TGVMIESNIN EGNQGIPAEG KAGLKYGVSI TDACIGWETT EDVLRKLAAA 

       370 
VRQRREVNKK 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, primary structure and regulation of the ARO4 gene, encoding the tyrosine-inhibited 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Saccharomyces cerevisiae."
Kuenzler M., Paravicini G., Egli C., Irniger S., Braus G.H.
Gene 113:67-74(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Kuenzler M.
Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 205-207.
[3]"The complete sequence of a 6794 bp segment located on the right arm of chromosome II of Saccharomyces cerevisiae. Finding of a putative dUTPase in a yeast."
Doignon F., Biteau N., Aigle M., Crouzet M.
Yeast 9:1131-1137(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Cloning, primary structure, and regulation of the HIS7 gene encoding a bifunctional glutamine amidotransferase: cyclase from Saccharomyces cerevisiae."
Kuenzler M., Balmelli T., Egli C.M., Paravicini G., Braus G.H.
J. Bacteriol. 175:5548-5558(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 352-370.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[10]"Evolution of feedback-inhibited beta/alpha barrel isoenzymes by gene duplication and a single mutation."
Hartmann M., Schneider T.R., Pfeil A., Heinrich G., Lipscomb W.N., Braus G.H.
Proc. Natl. Acad. Sci. U.S.A. 100:862-867(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 23-369.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X61107 Genomic DNA. Translation: CAA43419.1.
L20296 Genomic DNA. Translation: AAA65607.1.
Z36118 Genomic DNA. Translation: CAA85212.1.
BK006936 Genomic DNA. Translation: DAA07365.1.
PIRS38185.
RefSeqNP_009808.1. NM_001178597.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1HFBX-ray1.90A/B/C/D/E/F/G/H1-370[»]
1OABX-ray1.90A/B1-370[»]
1OF6X-ray2.10A/B/C/D/E/F/G/H1-370[»]
1OF8X-ray1.50A/B1-370[»]
1OFAX-ray2.01A/B1-370[»]
1OFBX-ray2.00A/B1-370[»]
1OFOX-ray1.85A/B1-370[»]
1OFPX-ray2.10A/B/C/D1-370[»]
1OFQX-ray2.70A/B/C/D1-370[»]
1OFRX-ray2.70A/B/C/D/E/F/G/H1-370[»]
1OG0X-ray2.70A/B/C/D/E/F/G/H1-370[»]
ProteinModelPortalP32449.
SMRP32449. Positions 23-370.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32944. 27 interactions.
DIPDIP-4175N.
IntActP32449. 6 interactions.
MINTMINT-534785.
STRING4932.YBR249C.

Proteomic databases

PaxDbP32449.
PeptideAtlasP32449.
PRIDEP32449.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR249C; YBR249C; YBR249C.
GeneID852551.
KEGGsce:YBR249C.

Organism-specific databases

CYGDYBR249c.
SGDS000000453. ARO4.

Phylogenomic databases

eggNOGCOG0722.
GeneTreeENSGT00390000001891.
HOGENOMHOG000220501.
KOK01626.
OMAVHIADEQ.
OrthoDBEOG76DV30.

Enzyme and pathway databases

BioCycYEAST:YBR249C-MONOMER.
UniPathwayUPA00053; UER00084.

Gene expression databases

GenevestigatorP32449.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR006218. DAHP1/KDSA.
IPR006219. DHAP_synth_1.
[Graphical view]
PANTHERPTHR21225. PTHR21225. 1 hit.
PfamPF00793. DAHP_synth_1. 1 hit.
[Graphical view]
PIRSFPIRSF001361. DAHP_synthase. 1 hit.
TIGRFAMsTIGR00034. aroFGH. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP32449.
NextBio971640.

Entry information

Entry nameAROG_YEAST
AccessionPrimary (citable) accession number: P32449
Secondary accession number(s): D6VQP5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1994
Last modified: March 19, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways