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P32449

- AROG_YEAST

UniProt

P32449 - AROG_YEAST

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Protein

Phospho-2-dehydro-3-deoxyheptonate aldolase, tyrosine-inhibited

Gene

ARO4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).

Catalytic activityi

Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.

Enzyme regulationi

Inhibited by tyrosine.

Pathwayi

GO - Molecular functioni

  1. 3-deoxy-7-phosphoheptulonate synthase activity Source: SGD

GO - Biological processi

  1. aromatic amino acid family biosynthetic process Source: UniProtKB-KW
  2. chorismate biosynthetic process Source: SGD
  3. response to stress Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Stress response

Enzyme and pathway databases

BioCyciYEAST:YBR249C-MONOMER.
UniPathwayiUPA00053; UER00084.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospho-2-dehydro-3-deoxyheptonate aldolase, tyrosine-inhibited (EC:2.5.1.54)
Alternative name(s):
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
DAHP synthase
Phospho-2-keto-3-deoxyheptonate aldolase
Gene namesi
Name:ARO4
Ordered Locus Names:YBR249C
ORF Names:YBR1701
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR249c.
SGDiS000000453. ARO4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 370369Phospho-2-dehydro-3-deoxyheptonate aldolase, tyrosine-inhibitedPRO_0000140851Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP32449.
PaxDbiP32449.
PeptideAtlasiP32449.
PRIDEiP32449.

Expressioni

Inductioni

By amino acid starvation.

Gene expression databases

GenevestigatoriP32449.

Interactioni

Protein-protein interaction databases

BioGridi32944. 28 interactions.
DIPiDIP-4175N.
IntActiP32449. 6 interactions.
MINTiMINT-534785.
STRINGi4932.YBR249C.

Structurei

Secondary structure

1
370
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi25 – 295
Helixi34 – 407
Helixi45 – 6218
Beta strandi68 – 747
Helixi81 – 9818
Turni99 – 1013
Beta strandi102 – 1076
Beta strandi115 – 1184
Turni122 – 1243
Beta strandi128 – 1303
Helixi134 – 14916
Turni150 – 1523
Beta strandi155 – 1584
Beta strandi161 – 1633
Helixi165 – 1684
Helixi169 – 1713
Beta strandi173 – 1775
Turni179 – 1835
Helixi185 – 1928
Beta strandi198 – 2014
Helixi209 – 21810
Beta strandi223 – 2275
Beta strandi231 – 2388
Beta strandi244 – 2485
Helixi259 – 26810
Beta strandi276 – 2816
Helixi282 – 2854
Helixi289 – 2913
Helixi292 – 30413
Beta strandi309 – 3179
Beta strandi319 – 3235
Helixi330 – 3334
Beta strandi339 – 3424
Helixi347 – 36721

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HFBX-ray1.90A/B/C/D/E/F/G/H1-370[»]
1OABX-ray1.90A/B1-370[»]
1OF6X-ray2.10A/B/C/D/E/F/G/H1-370[»]
1OF8X-ray1.50A/B1-370[»]
1OFAX-ray2.01A/B1-370[»]
1OFBX-ray2.00A/B1-370[»]
1OFOX-ray1.85A/B1-370[»]
1OFPX-ray2.10A/B/C/D1-370[»]
1OFQX-ray2.70A/B/C/D1-370[»]
1OFRX-ray2.70A/B/C/D/E/F/G/H1-370[»]
1OG0X-ray2.70A/B/C/D/E/F/G/H1-370[»]
ProteinModelPortaliP32449.
SMRiP32449. Positions 23-370.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32449.

Family & Domainsi

Sequence similaritiesi

Belongs to the class-I DAHP synthase family.Curated

Phylogenomic databases

eggNOGiCOG0722.
GeneTreeiENSGT00390000001891.
HOGENOMiHOG000220501.
InParanoidiP32449.
KOiK01626.
OMAiQYMSELV.
OrthoDBiEOG76DV30.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006218. DAHP1/KDSA.
IPR006219. DHAP_synth_1.
[Graphical view]
PANTHERiPTHR21225. PTHR21225. 1 hit.
PfamiPF00793. DAHP_synth_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001361. DAHP_synthase. 1 hit.
TIGRFAMsiTIGR00034. aroFGH. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32449 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSESPMFAAN GMPKVNQGAE EDVRILGYDP LASPALLQVQ IPATPTSLET
60 70 80 90 100
AKRGRREAID IITGKDDRVL VIVGPCSIHD LEAAQEYALR LKKLSDELKG
110 120 130 140 150
DLSIIMRAYL EKPRTTVGWK GLINDPDVNN TFNINKGLQS ARQLFVNLTN
160 170 180 190 200
IGLPIGSEML DTISPQYLAD LVSFGAIGAR TTESQLHREL ASGLSFPVGF
210 220 230 240 250
KNGTDGTLNV AVDACQAAAH SHHFMGVTKH GVAAITTTKG NEHCFVILRG
260 270 280 290 300
GKKGTNYDAK SVAEAKAQLP AGSNGLMIDY SHGNSNKDFR NQPKVNDVVC
310 320 330 340 350
EQIANGENAI TGVMIESNIN EGNQGIPAEG KAGLKYGVSI TDACIGWETT
360 370
EDVLRKLAAA VRQRREVNKK
Length:370
Mass (Da):39,749
Last modified:February 1, 1994 - v2
Checksum:i594ED48F24175979
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61107 Genomic DNA. Translation: CAA43419.1.
L20296 Genomic DNA. Translation: AAA65607.1.
Z36118 Genomic DNA. Translation: CAA85212.1.
BK006936 Genomic DNA. Translation: DAA07365.1.
PIRiS38185.
RefSeqiNP_009808.1. NM_001178597.1.

Genome annotation databases

EnsemblFungiiYBR249C; YBR249C; YBR249C.
GeneIDi852551.
KEGGisce:YBR249C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61107 Genomic DNA. Translation: CAA43419.1 .
L20296 Genomic DNA. Translation: AAA65607.1 .
Z36118 Genomic DNA. Translation: CAA85212.1 .
BK006936 Genomic DNA. Translation: DAA07365.1 .
PIRi S38185.
RefSeqi NP_009808.1. NM_001178597.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1HFB X-ray 1.90 A/B/C/D/E/F/G/H 1-370 [» ]
1OAB X-ray 1.90 A/B 1-370 [» ]
1OF6 X-ray 2.10 A/B/C/D/E/F/G/H 1-370 [» ]
1OF8 X-ray 1.50 A/B 1-370 [» ]
1OFA X-ray 2.01 A/B 1-370 [» ]
1OFB X-ray 2.00 A/B 1-370 [» ]
1OFO X-ray 1.85 A/B 1-370 [» ]
1OFP X-ray 2.10 A/B/C/D 1-370 [» ]
1OFQ X-ray 2.70 A/B/C/D 1-370 [» ]
1OFR X-ray 2.70 A/B/C/D/E/F/G/H 1-370 [» ]
1OG0 X-ray 2.70 A/B/C/D/E/F/G/H 1-370 [» ]
ProteinModelPortali P32449.
SMRi P32449. Positions 23-370.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32944. 28 interactions.
DIPi DIP-4175N.
IntActi P32449. 6 interactions.
MINTi MINT-534785.
STRINGi 4932.YBR249C.

Proteomic databases

MaxQBi P32449.
PaxDbi P32449.
PeptideAtlasi P32449.
PRIDEi P32449.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR249C ; YBR249C ; YBR249C .
GeneIDi 852551.
KEGGi sce:YBR249C.

Organism-specific databases

CYGDi YBR249c.
SGDi S000000453. ARO4.

Phylogenomic databases

eggNOGi COG0722.
GeneTreei ENSGT00390000001891.
HOGENOMi HOG000220501.
InParanoidi P32449.
KOi K01626.
OMAi QYMSELV.
OrthoDBi EOG76DV30.

Enzyme and pathway databases

UniPathwayi UPA00053 ; UER00084 .
BioCyci YEAST:YBR249C-MONOMER.

Miscellaneous databases

EvolutionaryTracei P32449.
NextBioi 971640.

Gene expression databases

Genevestigatori P32449.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR006218. DAHP1/KDSA.
IPR006219. DHAP_synth_1.
[Graphical view ]
PANTHERi PTHR21225. PTHR21225. 1 hit.
Pfami PF00793. DAHP_synth_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF001361. DAHP_synthase. 1 hit.
TIGRFAMsi TIGR00034. aroFGH. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, primary structure and regulation of the ARO4 gene, encoding the tyrosine-inhibited 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Saccharomyces cerevisiae."
    Kuenzler M., Paravicini G., Egli C., Irniger S., Braus G.H.
    Gene 113:67-74(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Kuenzler M.
    Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 205-207.
  3. "The complete sequence of a 6794 bp segment located on the right arm of chromosome II of Saccharomyces cerevisiae. Finding of a putative dUTPase in a yeast."
    Doignon F., Biteau N., Aigle M., Crouzet M.
    Yeast 9:1131-1137(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Cloning, primary structure, and regulation of the HIS7 gene encoding a bifunctional glutamine amidotransferase: cyclase from Saccharomyces cerevisiae."
    Kuenzler M., Balmelli T., Egli C.M., Paravicini G., Braus G.H.
    J. Bacteriol. 175:5548-5558(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 352-370.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  10. "Evolution of feedback-inhibited beta/alpha barrel isoenzymes by gene duplication and a single mutation."
    Hartmann M., Schneider T.R., Pfeil A., Heinrich G., Lipscomb W.N., Braus G.H.
    Proc. Natl. Acad. Sci. U.S.A. 100:862-867(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 23-369.

Entry informationi

Entry nameiAROG_YEAST
AccessioniPrimary (citable) accession number: P32449
Secondary accession number(s): D6VQP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1994
Last modified: October 29, 2014
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 26300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3