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P32445 (RIM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Single-stranded DNA-binding protein RIM1, mitochondrial
Alternative name(s):
Mitochondrial ssDNA-binding protein
Gene names
Name:RIM1
Ordered Locus Names:YCR028C-A
ORF Names:YCR28C-A
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length135 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein binds preferentially and cooperatively to ss-DNA. Involved in mitochondrial DNA replication.

Subunit structure

Homotetramer.

Subcellular location

Mitochondrion.

Miscellaneous

Present with 3060 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 SSB domain.

Ontologies

Keywords
   Biological processDNA replication
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandDNA-binding
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processmitochondrial DNA replication

Inferred from sequence or structural similarity Ref.1. Source: SGD

   Cellular_componentmitochondrial nucleoid

Inferred from direct assay PubMed 10869431. Source: SGD

   Molecular_functionsingle-stranded DNA binding

Inferred from direct assay Ref.1. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 1717Mitochondrion
Chain18 – 135118Single-stranded DNA-binding protein RIM1, mitochondrial
PRO_0000033262

Regions

Domain19 – 11799SSB

Sequences

Sequence LengthMass (Da)Tools
P32445 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 9F913C2FC9EF6E82

FASTA13515,386
        10         20         30         40         50         60 
MFLRTQARFF HATTKKMDFS KMSIVGRIGS EFTEHTSANN NRYLKYSIAS QPRRDGQTNW 

        70         80         90        100        110        120 
YNITVFNEPQ INFLTEYVRK GALVYVEADA ANYVFERDDG SKGTTLSLVQ KDINLLKNGK 

       130 
KLEDAEGQEN AASSE

« Hide

References

« Hide 'large scale' references
[1]"A single-stranded DNA binding protein required for mitochondrial DNA replication in S. cerevisiae is homologous to E. coli SSB."
van Dyck E., Foury F., Stillman B., Brill S.J.
EMBO J. 11:3421-3430(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PARTIAL PROTEIN SEQUENCE.
[2]"Nucleotide sequence of 9.2 kb left of CRY1 on yeast chromosome III from strain AB972: evidence for a Ty insertion and functional analysis of open reading frame YCR28."
Carbone M.L.A., Panzeri L., Falconi M.M., Carcano C., Plevani P., Lucchini G.
Yeast 8:805-812(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]"The complete DNA sequence of yeast chromosome III."
Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. expand/collapse author list , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		S43128 Genomic DNA. Translation: AAB22978.1.
S43129 mRNA. Translation: AAD13828.1.
X59720 Genomic DNA. Translation: CAA42321.2.
BK006937 Genomic DNA. Translation: DAA07508.1.
PIRS23548.
RefSeqNP_009958.2. NM_001184315.1.

3D structure databases

ProteinModelPortalP32445.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-7376N.
IntActP32445. 40 interactions.
MINTMINT-649905.

Proteomic databases

PaxDbP32445.
PeptideAtlasP32445.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYCR028C-A; YCR028C-A; YCR028C-A.
GeneID850395.
KEGGsce:YCR028C-A.

Organism-specific databases

CYGDYCR028c-a.
SGDS000007222. RIM1.

Phylogenomic databases

eggNOGCOG0629.
HOGENOMHOG000000901.
OMAKYSIASQ.
OrthoDBEOG4RZ28T.

Gene expression databases

GenevestigatorP32445.
GermOnlineYCR028C-A. Saccharomyces cerevisiae.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
InterProIPR012340. NA-bd_OB-fold.
IPR000424. Primosome_PriB/ssb.
IPR011344. ssDNA-bd.
[Graphical view]
PANTHERPTHR10302. PTHR10302. 1 hit.
PfamPF00436. SSB. 1 hit.
[Graphical view]
PIRSFPIRSF002070. SSB. 1 hit.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
PROSITEPS50935. SSB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio965920.

Entry information

Entry nameRIM1_YEAST
AccessionPrimary (citable) accession number: P32445
Secondary accession number(s): D6VR39, O11851
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: April 3, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome III

Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents