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P32445

- RIM1_YEAST

UniProt

P32445 - RIM1_YEAST

Protein

Single-stranded DNA-binding protein RIM1, mitochondrial

Gene

RIM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    This protein binds preferentially and cooperatively to single-stranded DNA (ssDNS). Involved in mitochondrial DNA replication. Stimulates PIF1 helicase activity.1 Publication

    GO - Molecular functioni

    1. single-stranded DNA binding Source: SGD

    GO - Biological processi

    1. mitochondrial DNA replication Source: SGD
    2. mitochondrial genome maintenance Source: SGD

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29415-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Single-stranded DNA-binding protein RIM1, mitochondrial
    Alternative name(s):
    Mitochondrial ssDNA-binding protein
    Gene namesi
    Name:RIM1
    Ordered Locus Names:YCR028C-A
    ORF Names:YCR28C-A
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCR028c-a.
    SGDiS000007222. RIM1.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial nucleoid Source: SGD
    2. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 1717MitochondrionAdd
    BLAST
    Chaini18 – 135118Single-stranded DNA-binding protein RIM1, mitochondrialPRO_0000033262Add
    BLAST

    Proteomic databases

    MaxQBiP32445.
    PaxDbiP32445.
    PeptideAtlasiP32445.

    Expressioni

    Gene expression databases

    GenevestigatoriP32445.

    Interactioni

    Subunit structurei

    Homotetramer. Interacts with PIF1.1 Publication

    Protein-protein interaction databases

    BioGridi31012. 187 interactions.
    DIPiDIP-7376N.
    IntActiP32445. 42 interactions.
    MINTiMINT-649905.

    Structurei

    3D structure databases

    ProteinModelPortaliP32445.
    SMRiP32445. Positions 17-116.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini19 – 11799SSBPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 SSB domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0629.
    HOGENOMiHOG000000901.
    OMAiFNEPQIN.
    OrthoDBiEOG7WT4D5.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    InterProiIPR012340. NA-bd_OB-fold.
    IPR000424. Primosome_PriB/ssb.
    IPR011344. ssDNA-bd.
    [Graphical view]
    PANTHERiPTHR10302. PTHR10302. 1 hit.
    PfamiPF00436. SSB. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002070. SSB. 1 hit.
    SUPFAMiSSF50249. SSF50249. 1 hit.
    PROSITEiPS50935. SSB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32445-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFLRTQARFF HATTKKMDFS KMSIVGRIGS EFTEHTSANN NRYLKYSIAS    50
    QPRRDGQTNW YNITVFNEPQ INFLTEYVRK GALVYVEADA ANYVFERDDG 100
    SKGTTLSLVQ KDINLLKNGK KLEDAEGQEN AASSE 135
    Length:135
    Mass (Da):15,386
    Last modified:October 1, 1993 - v1
    Checksum:i9F913C2FC9EF6E82
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S43128 Genomic DNA. Translation: AAB22978.1.
    S43129 mRNA. Translation: AAD13828.1.
    X59720 Genomic DNA. Translation: CAA42321.2.
    BK006937 Genomic DNA. Translation: DAA07508.1.
    PIRiS23548.
    RefSeqiNP_009958.2. NM_001184315.1.

    Genome annotation databases

    EnsemblFungiiYCR028C-A; YCR028C-A; YCR028C-A.
    GeneIDi850395.
    KEGGisce:YCR028C-A.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S43128 Genomic DNA. Translation: AAB22978.1 .
    S43129 mRNA. Translation: AAD13828.1 .
    X59720 Genomic DNA. Translation: CAA42321.2 .
    BK006937 Genomic DNA. Translation: DAA07508.1 .
    PIRi S23548.
    RefSeqi NP_009958.2. NM_001184315.1.

    3D structure databases

    ProteinModelPortali P32445.
    SMRi P32445. Positions 17-116.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31012. 187 interactions.
    DIPi DIP-7376N.
    IntActi P32445. 42 interactions.
    MINTi MINT-649905.

    Proteomic databases

    MaxQBi P32445.
    PaxDbi P32445.
    PeptideAtlasi P32445.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCR028C-A ; YCR028C-A ; YCR028C-A .
    GeneIDi 850395.
    KEGGi sce:YCR028C-A.

    Organism-specific databases

    CYGDi YCR028c-a.
    SGDi S000007222. RIM1.

    Phylogenomic databases

    eggNOGi COG0629.
    HOGENOMi HOG000000901.
    OMAi FNEPQIN.
    OrthoDBi EOG7WT4D5.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29415-MONOMER.

    Miscellaneous databases

    NextBioi 965920.

    Gene expression databases

    Genevestigatori P32445.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    InterProi IPR012340. NA-bd_OB-fold.
    IPR000424. Primosome_PriB/ssb.
    IPR011344. ssDNA-bd.
    [Graphical view ]
    PANTHERi PTHR10302. PTHR10302. 1 hit.
    Pfami PF00436. SSB. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002070. SSB. 1 hit.
    SUPFAMi SSF50249. SSF50249. 1 hit.
    PROSITEi PS50935. SSB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A single-stranded DNA binding protein required for mitochondrial DNA replication in S. cerevisiae is homologous to E. coli SSB."
      van Dyck E., Foury F., Stillman B., Brill S.J.
      EMBO J. 11:3421-3430(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PARTIAL PROTEIN SEQUENCE.
    2. "Nucleotide sequence of 9.2 kb left of CRY1 on yeast chromosome III from strain AB972: evidence for a Ty insertion and functional analysis of open reading frame YCR28."
      Carbone M.L.A., Panzeri L., Falconi M.M., Carcano C., Plevani P., Lucchini G.
      Yeast 8:805-812(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    3. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Physical and functional interaction between yeast Pif1 helicase and Rim1 single-stranded DNA binding protein."
      Ramanagoudr-Bhojappa R., Blair L.P., Tackett A.J., Raney K.D.
      Nucleic Acids Res. 41:1029-1046(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PIF1, SUBUNIT.

    Entry informationi

    Entry nameiRIM1_YEAST
    AccessioniPrimary (citable) accession number: P32445
    Secondary accession number(s): D6VR39, O11851
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3060 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3