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P32445

- RIM1_YEAST

UniProt

P32445 - RIM1_YEAST

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Protein

Single-stranded DNA-binding protein RIM1, mitochondrial

Gene

RIM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

This protein binds preferentially and cooperatively to single-stranded DNA (ssDNS). Involved in mitochondrial DNA replication. Stimulates PIF1 helicase activity.1 Publication

GO - Molecular functioni

  1. single-stranded DNA binding Source: SGD

GO - Biological processi

  1. mitochondrial DNA replication Source: SGD
  2. mitochondrial genome maintenance Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29415-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Single-stranded DNA-binding protein RIM1, mitochondrial
Alternative name(s):
Mitochondrial ssDNA-binding protein
Gene namesi
Name:RIM1
Ordered Locus Names:YCR028C-A
ORF Names:YCR28C-A
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCR028c-a.
SGDiS000007222. RIM1.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial nucleoid Source: SGD
  2. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1717MitochondrionAdd
BLAST
Chaini18 – 135118Single-stranded DNA-binding protein RIM1, mitochondrialPRO_0000033262Add
BLAST

Proteomic databases

MaxQBiP32445.
PaxDbiP32445.
PeptideAtlasiP32445.

Expressioni

Gene expression databases

GenevestigatoriP32445.

Interactioni

Subunit structurei

Homotetramer. Interacts with PIF1.1 Publication

Protein-protein interaction databases

BioGridi31012. 187 interactions.
DIPiDIP-7376N.
IntActiP32445. 42 interactions.
MINTiMINT-649905.

Structurei

3D structure databases

ProteinModelPortaliP32445.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 11799SSBPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 SSB domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0629.
HOGENOMiHOG000000901.
InParanoidiP32445.
OMAiFNEPQIN.
OrthoDBiEOG7WT4D5.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR000424. Primosome_PriB/ssb.
IPR011344. ssDNA-bd.
[Graphical view]
PANTHERiPTHR10302. PTHR10302. 1 hit.
PfamiPF00436. SSB. 1 hit.
[Graphical view]
PIRSFiPIRSF002070. SSB. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.
PROSITEiPS50935. SSB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32445-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFLRTQARFF HATTKKMDFS KMSIVGRIGS EFTEHTSANN NRYLKYSIAS
60 70 80 90 100
QPRRDGQTNW YNITVFNEPQ INFLTEYVRK GALVYVEADA ANYVFERDDG
110 120 130
SKGTTLSLVQ KDINLLKNGK KLEDAEGQEN AASSE
Length:135
Mass (Da):15,386
Last modified:October 1, 1993 - v1
Checksum:i9F913C2FC9EF6E82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S43128 Genomic DNA. Translation: AAB22978.1.
S43129 mRNA. Translation: AAD13828.1.
X59720 Genomic DNA. Translation: CAA42321.2.
BK006937 Genomic DNA. Translation: DAA07508.1.
PIRiS23548.
RefSeqiNP_009958.2. NM_001184315.1.

Genome annotation databases

EnsemblFungiiYCR028C-A; YCR028C-A; YCR028C-A.
GeneIDi850395.
KEGGisce:YCR028C-A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S43128 Genomic DNA. Translation: AAB22978.1 .
S43129 mRNA. Translation: AAD13828.1 .
X59720 Genomic DNA. Translation: CAA42321.2 .
BK006937 Genomic DNA. Translation: DAA07508.1 .
PIRi S23548.
RefSeqi NP_009958.2. NM_001184315.1.

3D structure databases

ProteinModelPortali P32445.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31012. 187 interactions.
DIPi DIP-7376N.
IntActi P32445. 42 interactions.
MINTi MINT-649905.

Proteomic databases

MaxQBi P32445.
PaxDbi P32445.
PeptideAtlasi P32445.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCR028C-A ; YCR028C-A ; YCR028C-A .
GeneIDi 850395.
KEGGi sce:YCR028C-A.

Organism-specific databases

CYGDi YCR028c-a.
SGDi S000007222. RIM1.

Phylogenomic databases

eggNOGi COG0629.
HOGENOMi HOG000000901.
InParanoidi P32445.
OMAi FNEPQIN.
OrthoDBi EOG7WT4D5.

Enzyme and pathway databases

BioCyci YEAST:G3O-29415-MONOMER.

Miscellaneous databases

NextBioi 965920.

Gene expression databases

Genevestigatori P32445.

Family and domain databases

Gene3Di 2.40.50.140. 1 hit.
InterProi IPR012340. NA-bd_OB-fold.
IPR000424. Primosome_PriB/ssb.
IPR011344. ssDNA-bd.
[Graphical view ]
PANTHERi PTHR10302. PTHR10302. 1 hit.
Pfami PF00436. SSB. 1 hit.
[Graphical view ]
PIRSFi PIRSF002070. SSB. 1 hit.
SUPFAMi SSF50249. SSF50249. 1 hit.
PROSITEi PS50935. SSB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A single-stranded DNA binding protein required for mitochondrial DNA replication in S. cerevisiae is homologous to E. coli SSB."
    van Dyck E., Foury F., Stillman B., Brill S.J.
    EMBO J. 11:3421-3430(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PARTIAL PROTEIN SEQUENCE.
  2. "Nucleotide sequence of 9.2 kb left of CRY1 on yeast chromosome III from strain AB972: evidence for a Ty insertion and functional analysis of open reading frame YCR28."
    Carbone M.L.A., Panzeri L., Falconi M.M., Carcano C., Plevani P., Lucchini G.
    Yeast 8:805-812(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  3. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Physical and functional interaction between yeast Pif1 helicase and Rim1 single-stranded DNA binding protein."
    Ramanagoudr-Bhojappa R., Blair L.P., Tackett A.J., Raney K.D.
    Nucleic Acids Res. 41:1029-1046(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PIF1, SUBUNIT.

Entry informationi

Entry nameiRIM1_YEAST
AccessioniPrimary (citable) accession number: P32445
Secondary accession number(s): D6VR39, O11851
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 26, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3060 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3