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Protein

Single-stranded DNA-binding protein RIM1, mitochondrial

Gene

RIM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein binds preferentially and cooperatively to single-stranded DNA (ssDNS). Involved in mitochondrial DNA replication. Stimulates PIF1 helicase activity.1 Publication

Miscellaneous

Present with 3060 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • single-stranded DNA binding Source: SGD

GO - Biological processi

  • mitochondrial DNA replication Source: SGD
  • mitochondrial genome maintenance Source: SGD
  • positive regulation of mitochondrial DNA replication Source: SGD

Keywordsi

Molecular functionDNA-binding
Biological processDNA replication

Enzyme and pathway databases

BioCyciYEAST:G3O-29415-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Single-stranded DNA-binding protein RIM1, mitochondrial
Alternative name(s):
Mitochondrial ssDNA-binding protein
Gene namesi
Name:RIM1
Ordered Locus Names:YCR028C-A
ORF Names:YCR28C-A
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCR028C-A.
SGDiS000007222. RIM1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 17MitochondrionAdd BLAST17
ChainiPRO_000003326218 – 135Single-stranded DNA-binding protein RIM1, mitochondrialAdd BLAST118

Proteomic databases

MaxQBiP32445.
PRIDEiP32445.
TopDownProteomicsiP32445.

Interactioni

Subunit structurei

Homotetramer. Interacts with PIF1.1 Publication

Protein-protein interaction databases

BioGridi31012. 411 interactors.
DIPiDIP-7376N.
IntActiP32445. 46 interactors.
MINTiMINT-649905.
STRINGi4932.YCR028C-A.

Structurei

3D structure databases

ProteinModelPortaliP32445.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 117SSBPROSITE-ProRule annotationAdd BLAST99

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000000901.
InParanoidiP32445.
OMAiQYVRKGA.
OrthoDBiEOG092C5PM6.

Family and domain databases

CDDicd04496. SSB_OBF. 1 hit.
InterProiView protein in InterPro
IPR012340. NA-bd_OB-fold.
IPR000424. Primosome_PriB/ssb.
IPR011344. ssDNA-bd.
PANTHERiPTHR10302:SF0. PTHR10302:SF0. 1 hit.
PfamiView protein in Pfam
PF00436. SSB. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.
PROSITEiView protein in PROSITE
PS50935. SSB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32445-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLRTQARFF HATTKKMDFS KMSIVGRIGS EFTEHTSANN NRYLKYSIAS
60 70 80 90 100
QPRRDGQTNW YNITVFNEPQ INFLTEYVRK GALVYVEADA ANYVFERDDG
110 120 130
SKGTTLSLVQ KDINLLKNGK KLEDAEGQEN AASSE
Length:135
Mass (Da):15,386
Last modified:October 1, 1993 - v1
Checksum:i9F913C2FC9EF6E82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S43128 Genomic DNA. Translation: AAB22978.1.
S43129 mRNA. Translation: AAD13828.1.
X59720 Genomic DNA. Translation: CAA42321.2.
BK006937 Genomic DNA. Translation: DAA07508.1.
PIRiS23548.
RefSeqiNP_009958.2. NM_001184315.1.

Genome annotation databases

EnsemblFungiiCAA42321; CAA42321; CAA42321.
YCR028C-A; YCR028C-A; YCR028C-A.
GeneIDi850395.
KEGGisce:YCR028C-A.

Similar proteinsi

Entry informationi

Entry nameiRIM1_YEAST
AccessioniPrimary (citable) accession number: P32445
Secondary accession number(s): D6VR39, O11851
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 22, 2017
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names