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Protein

Homeobox protein MOX-2

Gene

Meox2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Mesodermal transcription factor that plays a key role in somitogenesis and is required for sclerotome development. Activates expression of CDKN1A and CDKN2A in endothelial cells, acting as a regulator of vascular cell proliferation. While it activates CDKN1A in a DNA-dependent manner, it activates CDKN2A in a DNA-independent manner. May have a regulatory role when quiescent vascular smooth muscle cells reenter the cell cycle.By similarity2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi186 – 24560HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: MGI
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: MGI
  3. RNA polymerase II distal enhancer sequence-specific DNA binding Source: MGI
  4. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: MGI
  5. sequence-specific DNA binding Source: UniProtKB
  6. sequence-specific DNA binding transcription factor activity Source: UniProtKB

GO - Biological processi

  1. angiogenesis Source: MGI
  2. limb development Source: MGI
  3. neuron death Source: MGI
  4. palate development Source: MGI
  5. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  6. skeletal muscle tissue development Source: MGI
  7. somite specification Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein MOX-2
Alternative name(s):
Mesenchyme homeobox 2
Gene namesi
Name:Meox2
Synonyms:Gax, Mox-2, Mox2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:103219. Meox2.

Subcellular locationi

Nucleus By similarity. Nucleus speckle PROSITE-ProRule annotation

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. nuclear speck Source: UniProtKB-SubCell
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice display defective differentiation and morphogenesis of the limb muscles (PubMed:12925591). Mice lacking Meox1 and Meox2 show extremely disrupted somite morphogenesis, patterning and differentiation. They lack an axial skeleton and skeletal muscles are severely deficient (PubMed:12925591).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 303303Homeobox protein MOX-2PRO_0000049198Add
BLAST

Proteomic databases

PRIDEiP32443.

PTM databases

PhosphoSiteiP32443.

Expressioni

Developmental stagei

It is not expressed before 8-8.5 dpc. At 8-8.5 dpc it is found on the entire epithelium of the somite. At 9.5 dpc its expression is restricted to the sclerotome. At 10.5 dpc it is found in sclerotomally derived cells including the vertebral and costal precursors.1 Publication

Gene expression databases

BgeeiP32443.
CleanExiMM_MEOX2.
GenevestigatoriP32443.

Interactioni

Subunit structurei

Interacts with RNF10.By similarity

Protein-protein interaction databases

BioGridi201395. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliP32443.
SMRiP32443. Positions 193-243.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi42 – 476Poly-Ser
Compositional biasi63 – 8523Gln/His-rich (OPA repeat)Add
BLAST
Compositional biasi68 – 7912Poly-HisAdd
BLAST
Compositional biasi80 – 856Poly-Gln

Domaini

The polyhistidine repeat may act as a targeting signal to nuclear speckles.By similarity

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG250192.
GeneTreeiENSGT00760000118940.
HOGENOMiHOG000230987.
HOVERGENiHBG052456.
InParanoidiP32443.
KOiK09322.
OMAiSWHIPQM.
OrthoDBiEOG7JDQZZ.
PhylomeDBiP32443.
TreeFamiTF351603.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32443-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEHPLFGCLR SPHATAQGLH PFSQSSLALH GRSDHMSYPE LSTSSSSCII
60 70 80 90 100
AGYPNEEGMF ASQHHRGHHH HHHHHHHHHQ QQQHQALQSN WHLPQMSSPP
110 120 130 140 150
SAARHSLCLQ PDSGGPPELG SSPPVLCSNS SSLGSSTPTG AACAPGDYGR
160 170 180 190 200
QALSPADVEK RSGSKRKSDS SDSQEGNYKS EVNSKPRKER TAFTKEQIRE
210 220 230 240 250
LEAEFAHHNY LTRLRRYEIA VNLDLTERQV KVWFQNRRMK WKRVKGGQQG
260 270 280 290 300
AAAREKELVN VKKGTLLPSE LSGIGAATLQ QTGDSLANED SRDSDHSSEH

AHL
Length:303
Mass (Da):33,506
Last modified:September 30, 1993 - v1
Checksum:i41BD05FC39AA4427
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z16406 mRNA. Translation: CAA78899.1.
AK028352 mRNA. Translation: BAC25898.1.
AK031152 mRNA. Translation: BAC27282.1.
AK142690 mRNA. Translation: BAE25163.1.
S79168 Genomic DNA. No translation available.
CCDSiCCDS25888.1.
PIRiB49122.
RefSeqiNP_032610.1. NM_008584.3.
UniGeneiMm.341398.

Genome annotation databases

EnsembliENSMUST00000041183; ENSMUSP00000043587; ENSMUSG00000036144.
GeneIDi17286.
KEGGimmu:17286.
UCSCiuc007nkf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z16406 mRNA. Translation: CAA78899.1.
AK028352 mRNA. Translation: BAC25898.1.
AK031152 mRNA. Translation: BAC27282.1.
AK142690 mRNA. Translation: BAE25163.1.
S79168 Genomic DNA. No translation available.
CCDSiCCDS25888.1.
PIRiB49122.
RefSeqiNP_032610.1. NM_008584.3.
UniGeneiMm.341398.

3D structure databases

ProteinModelPortaliP32443.
SMRiP32443. Positions 193-243.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201395. 4 interactions.

PTM databases

PhosphoSiteiP32443.

Proteomic databases

PRIDEiP32443.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041183; ENSMUSP00000043587; ENSMUSG00000036144.
GeneIDi17286.
KEGGimmu:17286.
UCSCiuc007nkf.1. mouse.

Organism-specific databases

CTDi4223.
MGIiMGI:103219. Meox2.

Phylogenomic databases

eggNOGiNOG250192.
GeneTreeiENSGT00760000118940.
HOGENOMiHOG000230987.
HOVERGENiHBG052456.
InParanoidiP32443.
KOiK09322.
OMAiSWHIPQM.
OrthoDBiEOG7JDQZZ.
PhylomeDBiP32443.
TreeFamiTF351603.

Miscellaneous databases

NextBioi291796.
PROiP32443.
SOURCEiSearch...

Gene expression databases

BgeeiP32443.
CleanExiMM_MEOX2.
GenevestigatoriP32443.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mox-1 and Mox-2 define a novel homeobox gene subfamily and are differentially expressed during early mesodermal patterning in mouse embryos."
    Candia A.F., Hu J., Crosby J., Lalley P.A., Noden D., Nadeau J.H., Wright C.V.E.
    Development 116:1123-1136(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
  2. "Amino acid sequence of Mox-2 and comparison to its Xenopus and rat homologs."
    Candia A.F., Kovalik J.-P., Wright C.V.E.
    Nucleic Acids Res. 21:4982-4982(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Forelimb, Heart and Placenta.
  4. "Regulation of Gax homeobox gene transcription by a combination of positive factors including myocyte-specific enhancer factor 2."
    Andres V., Fisher S., Wearsch P., Walsh K.
    Mol. Cell. Biol. 15:4272-4281(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
  5. "The concerted action of Meox homeobox genes is required upstream of genetic pathways essential for the formation, patterning and differentiation of somites."
    Mankoo B.S., Skuntz S., Harrigan I., Grigorieva E., Candia A., Wright C.V., Arnheiter H., Pachnis V.
    Development 130:4655-4664(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiMEOX2_MOUSE
AccessioniPrimary (citable) accession number: P32443
Secondary accession number(s): Q544T6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 30, 1993
Last sequence update: September 30, 1993
Last modified: February 3, 2015
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.