Reviewed, UniProtKB/Swiss-Prot P32441 (HMD_METWO)

Names and origin

Protein names

Recommended name:
    5,10-methenyltetrahydromethanopterin hydrogenase
    EC=1.12.98.2
Alternative name(s):
    H(2)-forming N(5),N(10)-methylenetetrahydromethanopterin dehydrogenase
    N(5),N(10)-methenyltetrahydromethanopterin hydrogenase
    H(2)-dependent methylene-H(4)MPT dehydrogenase

Gene names

Name:

hmd

Organism

Methanobacterium wolfei

Taxonomic identifier

145261 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	19 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the reversible reduction of methenyl-H4MPT+ to methylene-H4MPT. HAMAP MF_01090
Catalytic activity	H2 + 5,10-methenyltetrahydromethanopterin = H+ + 5,10-methylenetetrahydromethanopterin. HAMAP MF_01090
Pathway	One-carbon metabolism; methanogenesis from carbone dioxide; 5,10-methylene-H(4)MPT from 5,10-methenyl-H(4)MPT (hydrogen route): step 1/1. HAMAP MF_01090
Sequence similarities	Belongs to the HMD family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	N5,N10-methenyltetrahydromethanopterin hydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 19	›19	5,10-methenyltetrahydromethanopterin hydrogenase HAMAP MF_01090		PRO_0000218516
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P32441-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 0C17E9D7BF1F97C9	FASTA	19	1,911
	10 MKLAILGAGC YRTDAASGI

References

[1]

"H2-forming methylenetetrahydromethanopterin dehydrogenase, a novel type of hydrogenase without iron-sulfur clusters in methanogenic archaea." Zirngibl C., van Dongen W., Schwoerer B., von Buenau R., Richter M., Klein A., Thauer R.K. Eur. J. Biochem. 208:511-520(1992) [PubMed: 1521540] [Abstract] Cited for: PROTEIN SEQUENCE.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.12.98.2. 7575.
Family and domain databases
HAMAP	MF_01090. [Tree]
ProtoNet	Search...

Entry information

Entry name

HMD_METWO

Accession

Primary (citable) accession number: P32441

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 5, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)